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BACKGROUND: Heart rate variability biofeedback is a respiratory training method that uses slow and deep breathing at the resonant frequency to induce rhythmic, high-amplitude oscillations in the cardiovascular system, thereby stimulating and exercising the autonomic and baroreflex. However, current studies have not systematically reviewed how heart rate variability biofeedback modulates the autonomic function and produces effects. There is a lack of public understanding of the mechanism of heart rate variability biofeedback, and its application progress and scheme are not fully understood. OBJECTIVE: To review the existing experimental studies on the effects of heart rate variability biofeedback on symptoms in different populations at home and abroad and to introduce the mechanisms and advances in the application of heart rate variability biofeedback to modulate the autonomic nervous system. METHODS: “Heart rate variability biofeedback, resonance breathing, heart rate variability, autonomic nerve, breathing training, chronic diseases, mental illness, biofeedback” were used as Chinese or English keywords to search in CNKI, WanFang Database, PubMed, and Web of Science. A total of 72 core related papers were included according to the inclusion and exclusion criteria. RESULTS AND CONCLUSION: The body’s oscillation system and resonance system are essential for the effectiveness of heart rate variability biofeedback. Oscillations reflect the response to external stimuli and self-regulating reflex systems, while resonances involve synchronous oscillations that result in higher amplitude operations. The balance between sympathetic and parasympathetic nerves is crucial for maintaining a stable internal environment. Autonomic nervous system disorders are associated with reduced heart rate variability and are closely linked to the progression of related diseases. Heart rate variability biofeedback utilizes the resonance characteristics of the cardiovascular system, inducing rhythmic high-amplitude oscillations by employing deep slow breathing at the resonance frequency. This method improves the regulatory function of the sympathetic and parasympathetic system reflexes and enhances the balance regulation between the two systems. Two major mechanisms of cardiovascular system resonance are the baroreflex closed-loop pathway and respiratory sinus arrhythmia. These mechanisms, along with the unique delay of baroreflex, result in a 0° phase angle oscillation between heart rate and respiration and a 180° phase angle oscillation between blood pressure and respiration during breathing at the resonant frequency rhythm. Periodically stimulating the human cardiovascular oscillation system through this method is an easy-to-operate and effective training approach. Currently, heart rate variability biofeedback is mainly applied in the fields of mental illness, chronic disease, and sports. However, the intervention mechanism and efficacy are unclear, the intervention content, frequency and duration are varied, and there are limited review studies on the intervention methods tailored to different types of individuals. As a non-drug and non-invasive intervention, heart rate variability biofeedback can significantly increase heart rate variability, regulate the balance between sympathetic and parasympathetic nerves, and improve the stability and adaptability of the autonomic nervous system. In the future, it is suggested to investigate the mechanisms and potential applications of the pathways of the heart rate variability biofeedback that induce cardiovascular resonance. It is also recommended to incorporate long-term follow-ups to assess the sustained value of heart rate variability biofeedback in various fields. This would provide new directions and strategies for the comprehensive treatment of complex diseases. [ABSTRACT FROM AUTHOR]

Objective This investigation sought to delineate the associations among colorectal adenomatous polyps, diabetes, and biomolecules involved in glucose metabolism. Method Data were collected from 40 patients who underwent endoscopic polypectomy at the Endoscopy Department of Shandong Cancer Hospital between June 2019 and September 2021. This cohort included 27 patients with inflammatory polyps and 13 with adenomatous polyps. We assessed fasting insulin (Fins), fasting blood glucose (FBG), and the mRNA expressions of fibroblast growth factor 19 (FGF-19) and insulin-like growth factor 1 (IGF-1) in the polyp tissues. Both univariate and multivariate logistic regression analyses were employed to ascertain the determinants influencing the emergence of adenomatous polyps. From these analyses, a predictive nomogram was constructed to forecast the occurrence of adenomatous polyps, and evaluations on the discriminative capacity, calibration, and clinical utility of the model were conducted. Results The adenomatous polyp group exhibited markedly elevated levels of glucose, insulin, FGF-19, and IGF-1, with respective concentrations of (8.67±2.70) mmol/L, (12.72±7.69) μU/L, 2.20±1.88, and 1.36±0.69. These figures were significantly higher compared to the inflammatory polyp group, which showed levels of (5.51±0.72) mmol/L, (5.49±2.68) μU/L, 0.53±0.97, and 0.41±0.46, respectively, P= 0.001. Multivariate logistic regression revealed that the relative expression of IGF-1 served as an independent risk factor for the development of colorectal adenomatous polyps (OR=5.622, 95% CI: 1.085-29.126). The nomogram displayed a C-index of 0.849, indicating substantial discriminative capability. The calibration curve affirmed the model's accuracy in aligning predicted probabilities with actual outcomes, and the clinical decision curve demonstrated thepractical clinical applicability of the model. Conclusions There was a significant correlation between the occurrence of colorectal adenomatous polyps and glucose metabolic pathways. Individuals with diabetes showed a higher propensity to develop such polyps. [ABSTRACT FROM AUTHOR]

Mytilus is one of bivalves with great economic and ecological values. The innate immune defense of Mytilus shows great significance in the study of marine biological immunology. Hemolymph is the main immune tissue for Mytilus. The Nanopore full-length transcriptome of Mytilus coruscus hemocytes, and the serum differential proteomics based on SDS-PAGE analysis were performed to identify key proteins involving in the immune response of Mytilus hemolymph in response of different bacteria and fungi stresses. A total of 44 proteins were identified in the serum induced by different microorganisms. Among them, 26 proteins showed significant differential expression level in response to different microbial stresses, and their functions were involved in protein folding protection, cell autophagy and apoptosis regulation, reactive oxygen species production, energy metabolism regulation, cell detoxification, and immune regulation. The changes in expression levels of these proteins varied in response to different bacterial and fungal stresses, suggesting that Mytilus has different immune response strategies to different bacterial and fungal stresses. The results provide a new scientific basis for understanding the differential immune mechanism of Mytilus innate immune system in response to different types of microbial invasion, as well as the screening of specific biomarker proteins for microbial infection, and provide ideas for the healthy development and disease prevention of shellfish aquaculture. [ABSTRACT FROM AUTHOR]

The YOLOv5s-FCS network for traditional steel materials, which addresses issues such as false positives, false negatives, and low accuracy in detecting certain types of defects, is presented in this article. Firstly, the CBF convolution module is constructed using the FReLU activation function to enhance the network's spatial resolution capability and optimize detection accuracy. Secondly, a coordinate attention mechanism is embedded into the neck part of the network to enhance its feature fusion capability, enabling the extraction of more rich feature information. Finally, the SIoU loss replaces the YOLOv5s loss function to improve the regression accuracy of the predicted box. Through ablation experiments and visualization comparisons on the NEU-DET dataset, it is demonstrated that the mAP value of the YOLOv5s-FCS network reaches 0.747, representing an improvement of 8.3% compared to the original YOLOv5s network, 11.8% compared to the YOLOv3 network, 4.2% compared to the YOLOXs network, and 1.4% compared to the YOLOv6s network, thus demonstrating the feasibility and effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]

In order to explore the response law of lining under blasting vibration and the stress distribution state under blasting load when the blasting source gradually approaches the existing tunnel, combined with the Xishan Tunnel Project in Ouhai District, Wenzhou City, the safety of intersecting tunnels during the construction of new tunnel blasting was studied based on the blasting monitoring data and numerical simulation results at the construction site. The research findings indicate that vibration velocity is primarily influenced by the distance to the blasting center and the amount of explosive charge used. Specifically, as the blasting center distance decreases and the amount of explosive charge increases, the peak vibration velocity becomes higher. The blasting center distance determines the location of the peak lining speed within the tunnel. Simultaneously, this peak speed location corresponds to the point of maximum tensile stress. As the blasting center distance decreases, the critical point shifts from the arch waist position to the foot of the arch. [ABSTRACT FROM AUTHOR]

【Objective】To improve the softwood cutting technology system for Carya illinoensis (Wangench.) K. Koch,and identify the endogenous substances affecting cutting root development.【Methods】Three Carya illinoensis (Wangench.) K. Koch varieties,Caddo,Jinhua and Mahan,were used as experimental materials to study the rooting rate and root effect index of cuttings under different hormone types,shading rates,humidity levels,and container conditions through four-factor four-level orthogonal test. The changes in soluble sugar content,soluble protein content,IAA content,ABA content,IAA/ABA,POD activity,and SOD activity with the root effect index were analyzed.【Results】The order of influence of various factors on the cuttings′ rooting rate of Carya illinoensis (Wangench.) K. Koch were humidity level=shading rate>types of hormone>container conditions. Cuttings under IBA treatment,0 or 60% shading rate and 60%

Objective To study the effect of drinking carbohydrate drinks before cesarean section on mothers and neonates, and to explore the application value of drinking carbohydrate drinks before cesarean section. Methods The clinical data of 206 singleton women who underwent selective cesarean section in Obstetrics and Gynecology Hospital, Fudan University from Jun 2020 to Jun 2021 were retrospectively studied. Patients were divided into enhanced recovery after delivery (ERAD) group and control group according to whether drinking carbohydrate drinks before cesarean section. A retrospective cohort study was conducted to analyze the effect of preoperative carb drinks on preoperative fluid supplementation, postoperative rehabilitation and neonatal prognosis. Results Among patients who fasted for less than 12 hours, the ERAD group had a lower fluid supplementation rate and a smaller average fluid supplementation volume compared to the control group (P<0.05). The ERAD group had a lower rate of prokinetic agent using after surgery (P<0.05). Among women without a history of abdominal surgery, the ERAD group had less blood loss 24 hours after surgery( P<0.05). There were no significant differences in postoperative fever rate, incidence of nausea and vomiting, time of first flatus, neonatal apgar score, exit observation room neonatal blood, and neonatal neonatal intensive care unit (NICU) admission rate between the two groups. Among newborns with high-risk factors for hypoglycemia, the ERAD group had lower enter observation room neonatal blood compared to the control group, and a higher incidence of hypoglycemia (P<0.05). Conclusion Oral intake of carbohydrate drinks before cesarean section may be beneficial in reducing fluid supplementation before elective cesarean section, promoting postoperative gastrointestinal function recovery, and reducing postoperative bleeding. However, it may be related to the occurrence of neonatal hypoglycemia. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of manganese ion (Mn2+ ) on the proliferation and apoptosis of liver cancer cells. Methods Hepatoma Huh7 and HepG2.2.15 cells were treated with different concentrations (1, 4, 16, 64, 256 and 1 024 μmol/L, respectively) of Mn2+ solutions, and the corresponding cells without Mn2+ solution treatment were used as the control group. The cell proliferation was detected by CCK⁃8 assay and colony formation assay, and the apoptosis rate was detected by flow cytometry. Results The results of CCK⁃8 assay showed that compared with the control group, the cell proliferation activity of Huh7 cells was decreased under the treatment of all the conentrations of Mn2+ solution except the one of 1 μmol / L (all P<0.05); the proliferation activity of HepG2.2.15 cells was significantly decreased under the treatment of all the conentrations of Mn2+ solutions except those of 1 μmol/L and 4 μmol/L (all P<0.0001), and with the increase of Mn2+ concentration, the inhibition of cell proliferation in both groups was more and more significant. The results of colony formation assay showed that the colonies number of Huh7 cells was decreased under the treatment of all the Mn2+ solution except those of 1 μmol/L and 4 μmol/L (all P<0.01); the colonies number of HepG2.2.15 cells was decreased under the treatment of all the Mn2+ solution except the one of 1 μ mol/L (all P<0.0001), and with the increase of Mn2+ concentration, the colonies number of cell decreased significantly. The results of apoptosis experiment showed that the apoptosis rates of Huh7 cells and HepG2.2.15 cells treated with 64 μmol/L and 1 024 μmol/L Mn2+ solution (respectively) were significantly different (all P<0.01), and the apoptosis rates increased with the increase of Mn2+ concentration. Conclusion Mn2+ can inhibit the proliferation and colony formation of liver cancer cells and induce apoptosis. [ABSTRACT FROM AUTHOR]

Electrochemical mass spectrometry is an important technique for studying the mechanism of electrochemical reactions. Mass spectrometry has the advantages of high detection sensitivity, high detection throughput and high specificity, which enables simultaneous acquisition of qualitative and quantitative informations for multiple components, making it highly valuable in the monitoring of electrochemical reaction products and the identification of intermediates. In general, electrolyte is necessary for electrochemical reaction. However, these electrolyte salts are detrimental to mass spectrometry analysis as they not only cause condensation in the mass spectrometry inlet, leading to blockage, but also impede the signal of the target analyte or form adduct ions with target analyte, resulting in a complex mass spectrum and increased analytical challenges. It is of great significance to develop a mass spectrometry method to real-time obtain trace organic components in high concentration buffer salt solutions. Therefore, a novel atmospheric pressure chemical ionization mass spectrometry (APCI-MS) method was developed for online analysis of trace organic components in high concentration buffer salt solutions, and a small volume flow electrolytic cell, stable performance, and simple structure were fabricated for on-line electrochemical reaction, which employed Pt, Pt and Ag/AgCl as working electrode, counter electrode and reference electrode, respectively. After combination of the APCI-MS and flow electrolytic cell, an electrochemical mass spectrometry device and method for on-line analysis of the electrochemical process in high concentration buffer salt solutions were constructed. As a proof of concept application, the device and method were used to on-line and real-time study the electrochemical oxidation process of dopamine in phosphate buffer salt solutions. The experimental results showed that the APCI-MS method can efficiently remove the inorganic salts in solutions on-line by on-line thermal deposition, while the organic components can be ionized by APCI for mass spectrometry detection. The results of dopamine electrochemical oxidation analysis showed that dopamine was not oxidized under the oxidation potential at 0 V, and only the protonated dopamine m/z 154 [M+H]+ was obtained. When the oxidation potential was +0.3 V, in addition to the protonated dopamine, the protonated peak m/z 150 [M+H]+ of dopachrome was generated by losing 4e- from dopamine, and the protonated peak m/z 303 [M+H]+ of the adduct dopachrome and dopamine formed by the intermolecular hydrogen bonding were also obtained. When the oxidation potential increased to +0.6 V, the contents of oxidation products m/z 150 and m/z 303 were further increased. The results demonstrated that this method has great application potential in studying the electrochemical reaction. [ABSTRACT FROM AUTHOR]

目的 探讨血浆 C/EBP 同源蛋白 (CHOP) 、N-乙酰神经氨酸 (Neu5Ac) 水平与急性心肌梗死 (AMI) 患者经 皮冠状动脉介入治疗 (PCI) 术后预后的关系。方法 选取 146 例 AMI 患者为 AMI 组, 根据预后情况分为预后不良组 和预后良好组, 另选取同期 50 例体检健康者为对照组。酶联免疫吸附法检测血浆 CHOP、Neu5Ac。建立 AMI 患者 PCI 后预后不良的多因素 Logistic 回归模型, 绘制血浆 CHOP、Neu5Ac 水平预测 AMI 患者 PCI 后预后不良的受试者工 作特征 (ROC) 曲线。结果 与对照组比较, AMI 组血浆 CHOP、Neu5Ac 水平升高 (P 均<0. 05) 。随访 1 年, 146 例 AMI 患者预后不良发生率为 25. 34% (37/146) 。多因素 Logistic 回归分析显示, AMI 患者 PCI 后预后不良的独立危险因素 为 KILLIP 分级≥Ⅲ级和 CHOP、Neu5Ac (P 均<0. 05), 独立保护因素为左心室射血分数高 (P<0. 05) 。ROC 曲线分析 结果显示, 血浆 CHOP、Neu5Ac 和 CHOP 联合 Neu5Ac 预测 AMI 患者 PCI 后预后不良的曲线下面积分别为 0. 777、 0. 781、0. 854, CHOP 联合 Neu5Ac 预测 AMI 患者 PCI 后预后不良的曲线下面积高于单独指标检测 (P 均<0. 05) 。结 论 AMI 患者血浆 CHOP、Neu5Ac 水平升高, 与 PCI 后预后不良独立相关, 血浆 CHOP 联合 Neu5Ac 预测 AMI 患者 PCI 后预后不良的价值较高, 有望成为 AMI 患者 PCI 后预后辅助预测指标。 [ABSTRACT FROM AUTHOR]

Taking the inappropriate practices in existing research pointed out by a leading expert in clinical prediction models as examples, this paper introduced common misunderstandings in clinical prediction model research, explained why specific practices will jeopardize the model quality, and how to avoid repeating the same mistakes in future studies. The feasibility of using ChatGPT to provide methodological guidance is discussed by evaluating the answers given by ChatGPT. [ABSTRACT FROM AUTHOR]

Osteoporosis and osteoarthritis are common clinical aging-related bone metabolism disease, and their incidence gradually increases with age. Polydatin is a natural polyphenol and non-flavonoid compound present in the traditional Chinese medicine Polygonum cuspidatum, and it has attracted much attention because of its strong role in regulating bone metabolism and stimulating blood vessel formation. In this research, we summarize relevant literature that published at home or abroad, which contents about the characteristics of pharmacokinetic, pharmacological mechanisms and targets of polydatin in the prevention and treatment of osteoporosis and osteoarthritis. It is expected to provide more clues for the follow-up research on the prevention and treatment of aging-related bone metabolism diseases, and to supply more scientific theoretical basis for the development and utilization of polydatin. [ABSTRACT FROM AUTHOR]

分析宁夏回族自治区人民医院收治的1例患者口服氟康唑胶囊治疗白假丝酵母菌感染后发生 高血钾的诊疗过程,采用诺氏评估量表法分析高血钾与氟康唑之间的相关性。患者因阴囊分泌物培养检出白 假丝酵母菌,加用氟康唑抗真菌治疗,用药后第4天出现高血钾(6.52mmol/L)。经利尿和高糖胰岛素降钾对 症治疗并停用氟康唑后,多次检测血钾恢复正常(4.48mmol/L)。诺氏评估量表法显示氟康唑使用与高血钾 的发生很可能有关。高血钾是氟康唑新发现的不良反应,应用过程中应予以重视。 [ABSTRACT FROM AUTHOR]

【Objective】This study aimed to investigate the effects of rhizospheric NaCl concentration on the morphology and physiology of Mesembryanthemum crystallinum L. and determine the suitable NaCl concentration for it’s hydroponic cultivation in plant factories, providing references for production management.【Method】Crystal M. crystallinum L. seeds were used as material, and the Japanese garden general formula was selected for cultivation in nutrient solution under artificial light. With no NaCl added as the control (CK), NaCl was added to the final concentration of 3 g/L (T1), 6 g/L (T2), 9 g/L (T3), 12 g/L (T4), respectively. After 21 days of NaCl treatment, the morphological and physiological indicators were analyzed, and the proportion of epidermal salt vacuole area and the number of epidermal salt vacuoles were counted, so as to analyze the relationship between NaCl concentration and the morphology and physiology of M. crystallinum L..【Result】From the perspective of morphological indicators and biomass, there were no significant differences in plant height, stem thickness, leaf area, root fresh weight and shoot fresh weight compared to the CK when M. crystallinum L. was grown in a nutrient solution with a NaCl concentration of 3 g/L. In the nutrient solution with a NaCl concentration of 6 g/L, M. crystallinum L. showed significantly increased in plant height, root fresh weight and shoot fresh weight compared to the CK, indicating a certain promotion effect on the growth of M. crystallinum L.. However, in the nutrient solutions with NaCl concentrations of 9 g/L and 12 g/L, M. crystallinum L. exhibited a decreasing trend in plant height, stem thickness, leaf area, and shoot fresh weight with significant differences compared to the CK. This suggests that the growth of M. crystallinum L. is inhibited in nutrient solutions with NaCl concentrations of 9 g/L and 12 g/L. In terms of physiological indicators, the protein content, soluble sugar content, and chlorophyll SPAD value of M. crystallinum L. increased with increasing NaCl concentration, reaching the highest levels in the nutrient solution with a NaCl concentration of 12 g/L, with increases of 50.2%, 119.5%, and 77.9% compared to the CK, respectively. Regarding the epidermal salt vacuole indicators, both the proportion of epidermal salt vacuole area and the number of epidermal salt vacuoles increased with increasing NaCl concentration, reaching the maximum values in the nutrient solution with a NaCl concentration of 12 g/L, with increases of 54.9% and 182.1% compared to the CK, respectively.【Conclusion】The optimal NaCl concentration for hydroponic cultivation of M. crystallinum L. in plant factories falls within the range of 3-6 g/L. [ABSTRACT FROM AUTHOR]

Aiming at the problem that TextRank ignores syntactic information and topic information when extracting chapter keywords, propose a chapter keyword extraction model based on syntactic analysis and topic distribution. Model includes two stages of chapter keyword extraction: paragraph and chapter. Firstly, use paragraphs as a unit to extract paragraph keywords by combining word co-occurrence, grammatical and semantic information. Then cluster the paragraphs according to the paragraph topics to form the paragraph topic set. Finally, extract chapter keywords based on the distribution characteristics of paragraph topics. On the open news dataset, the model’s extraction effect improves by about 10% compared with the original TextRank. Results show that the method has significantly improved the extraction effect, and prove the importance of grammatical information and topic information. [ABSTRACT FROM AUTHOR]

The gut microbiota, as one of the microfloras existing in human body, is accounted for approximately 80% in human microbiome. Gut microbiota regulates digestive and immune functions through participating in physiological processes such as metabolism and immune homeostasis, meanwhile impacts the occurrence of cancer and controls the body response to cancer treatment drugs. Hepatocellular carcinoma (HCC) is a typical type of inflammatory cancer, the development of which follows the process of hepatitis, liver fibrosis, liver cirrhosis and HCC. The bidirectional communication system composed of gut microbiota, portal vein system and bile duct system, is usually called the enteric-liver axis. More and more research evidences show that gut microbiota can participate in the occurrence and development of HCC via the interaction cycle with the enteric-liver axis. In view of the specific transformation of gut microbiota in the early stage of HCC, it is usually regarded as a new target for the early diagnosis of HCC. Immunotherapy is an emerging treatment for advanced HCC. It is reported that regulating gut microbiota through probiotics, rational application of antibiotics, fecal transplantation and other methods can significantly improve the anti-tumor immune response of host. In the non-immunotherapy process of HCC chemotherapy, traditional Chinese medicine-assisted therapy and dietary pattern, remodeling gut microbiota homeostasis has potential value in postponing tumor progression, improving the prognosis of HCC treatment and maintaining body health. Since gut microbiota plays a crucial role in the occurrence, development and treatment of HCC, this review summarizes the studies of gut microbiota in the process of hepatitis, liver fibrosis, cirrhosis and HCC, and discusses its great potential as a target for the prevention, diagnosis and treatment of HCC. [ABSTRACT FROM AUTHOR]

Exploring the mechanical properties of vegetation root is of great significance for how to evaluate the performance of vegetation slope protection in the process of preventing geological disasters such as landslides and debris flows in small watersheds in mountainous areas. Taking Elsholtzia, a common plant in the small watershed of Reshui River in Xide County, Liangshan Yi Autonomous Prefecture, Sichuan Province, as the research object, the in-situ pull-out test and indoor single root tensile test were carried out to obtain the mechanical properties of the root. The gray correlation analysis method was used to study the correlation between the root breadth, root length, root diameter, crown breadth, plant height of Elsholtzia and the in-situ pull-out resistance of Elsholtzia. Furthermore, the influences of the single root diameter, root length, and root water conditions of Elsholtzia on its indoor single root tensile strength were also analyzed. The results show that the correlation degree values between the different growth indexes of Elsholtzia and its in-situ pull-out resistance in descending order are root breadth, root length, root diameter, crown width and plant height, which means the in-situ pull-out resistance of Elsholtzia can be better characterized by the underground growth indexes（i.e., root breadth and root length）. The single root tensile force of Elsholtzia has a positive correlation with root diameter. The average tensile strength of the natural root and the immersed root generally decreases with an increasing of root length, as contrasted to the dry root. The average tensile strength of the root of Elsholtzia generally increases with the decrease of root moisture content. The above research results can provide a certain reference for ecological slope protection as well as soil and water conservation research in the small watershed in mountainous areas such as Reshui River. [ABSTRACT FROM AUTHOR]

To explore the nearly perfect structure of biomaterials and analyze the relationship between structural orientation and mechanical behavior, this paper started from the geometric orientation of the microstructure of biomaterials, based on the linear elastic structural constitutive relationship, the microstructure of two-phase biomimetic composites was established by numerical simulation method. The results show that the model can describe the properties of biomaterials effectively with a small computational error. When the Mises stress scale is the same, the orientation angle is directly proportional to the internal stress of the bionic material and inversely proportional to the stiffness of the bionic material, and the increase of the orientation angle leads to the uneven structure of the bionic material. The results provide the theoretical basis for the study of the mechanical behavior of biomimetic composites and engineering applications. [ABSTRACT FROM AUTHOR]

High viscosity modified asphalt was prepared with RST (Road Science Technology) modifier. The content of RST was 7%,9%,11%,13%,15% and 17%. The effect of RST content on the temperature sensitivity and storage stability of high viscosity modified asphalt was studied. Firstly, the penetration index (PI), penetration viscosity index (PV7V) and viscosity temperature index (VTS) of RST high viscosity modified asphalt were measured and calculated to evaluate the temperature sensitivity at different ambient temperatures; secondly, the softening point difference after storage at (163 ± 5) °C for 48 h was measured by the separation tube test method to evaluate the storage stability; finally, the modification mechanism was analyzed by fluorescence microscope. The results show that the temperature sensitivity of RST high viscosity modified asphalt is improved with the increase of modifier content under medium temperature and medium high temperature environment; in high temperature environment, the temperature sensitivity of high viscosity modified asphalt increases first and then decreases with the increase of RST content. When the content is less than 15%, RST can absorb the light components in asphalt and form a stable network structure to improve the storage stability of asphalt; when the content exceeds 15%, RST forms strip structure in asphalt and segregation occurs, resulting in poor storage stability. [ABSTRACT FROM AUTHOR]

As a piling platform of the new berth, the reserved structure of the high -pile wharf can be considered to be incorporated into the existing structure during the expansion of the berth. It is necessary to test the bearing capacity of the reserved structure, and judge the treatment of reserved structure with reference to the detection and evaluation results of component applicability and durability. Taking a reserved structure in the North Port area of Tianjin Port as an example, the process of in-situ surcharge test for testing the bearing capacity of active foundation piles in high-pile wharf was described, and the inspection method of component applicability and durability were introduced. The test and detection results show that the bearing capacity of the reserved structure meets the design requirements, and the concrete strength of the pile cap does not meet the requirements. Finally, it is decided to retain the foundation pile and dismantle all the superstructure, which provides a reference example for the optimal utilization of the reserved structure of high-pile wharf. [ABSTRACT FROM AUTHOR]

The E-Reserve service in the "digital campus" ecology is different from the traditional online E-Reserves service. This paper investigates the development of E-Reserves services in academic libraries at home and abroad and gives in-depth analysis of the characteristics of E-Reserves services, elaborates the de sign of Peking University Library's E-Reserves system from the perspective of information collaboration. In the context of "digital campus" construction, the system effectively integrates data from multiple sources through multi-departmental coordination and provides accurate and high-quality E-Reserves services for teachers and students. Based on actual data, the good results and successful experience of system construc tion and service development are summarized, and the future expansion and optimization of this service are prospected. [ABSTRACT FROM AUTHOR]

With the improvement of quality of life and changes of dietary habits, people's requirements for food should not only include food and clothing, but also color, aroma, taste, shape and meaning, in which food additives play a very important role. Titanium dioxide (TiO2) is often widely used as a pigment additive in food and daily necessities, so people will inevitably contact and ingest TiO2 nanomaterials in daily life. Based on this, the article reviews the effect of TiO2 nanomaterials on digestive tract tissues and gastrointestinal microbiome, as well as its direct and indirect pro-inflammatory effects in the occurrence and development of gastrointestinal inflammation, further providing reference improving the safety of TiO2 nanomaterials in the food field. [ABSTRACT FROM AUTHOR]

Atmospheric freeze drying (AFD) has removed the vacuum-creating equipment to reduce energy consumption while maintaining the product quality, compared with traditional vacuum freeze drying. However, the increasing pressure can lead to a significant increase in the drying time. Water vapor diffusion can then be obstructed to limit the practical implementation. Therefore, it is essential to understand the controlling variables in the heat and mass transfer of the AFD process. The purpose of this study was to quantitatively investigate the effects of drying conditions on the drying time, drying rate, and heat/mass transfer resistances. Among them, the thermal/mass transfer resistances were analyzed to explore the drying mechanism during atmospheric freeze drying. The mathematical model of the lamellar product during AFD was proposed to consider the external flow. The Navier–Stokes equations were introduced for the external pressure field into the adsorption-sublimation model. The model was solved and validated using COMSOL Multiphysics 6.1. The accuracy was verified by the ±10% relative deviation of drying time between the model and experimental data. Drying kinetics were calculated under different conditions (product thickness 3-11 mm, equivalent pore diameter 2.0-6.0 μm, dry air temperature 263.15-273.15 K, dry air velocity 1.0-3.0 m/s). The results showed that the drying rate and time decreased with the decrease in product thickness and the increase in air temperature. But there was no outstanding influence of equivalent pore diameter and air velocity on the drying rate and time. Unlike the three stages of standard drying, only the initial accelerating and falling rate stages were observed in the AFD procedure. The absence of the constant rate stage was attributed to the assumption of material homogeneity and low moisture of the top surface. At the same time, the post-processing was carried out to calculate the external heat and mass transfer resistances between the external flow and the product, together with the internal heat and mass transfer resistances between the top surface of the product and the sublimation interface. The external heat/mass transfer resistances decreased, while the internal heat/mass transfer resistances increased linearly from zero, with the decreasing moisture content. The internal heat/mass transfer resistances exceeded the external heat/mass transfer resistances at a critical moisture content, indicating the transition from external heat/mass transfer control to internal heat/mass transfer control during AFD. There was some effect of condition parameters on the transfer resistances. The equivalent pore diameter shared no significant effect on either internal or external resistances. While the temperature increased to bring about a slight decrease in the internal heat-transfer resistance. By contrast, there was a decrease in the external and internal heat transfer resistance, due to the increasing air velocity. Especially, the reduction of product thickness was a significant contribution to the decrease of both internal and external transfer resistance. Therefore, small thickness product was more suitable for the AFD process, according to the drying kinetics and heat/mass transfer. It was expected to simultaneously realize both the reduction in the drying time and energy consumption. The findings can provide guidance to optimize the process parameters during atmospheric freeze-drying. [ABSTRACT FROM AUTHOR]

As the original advantages of TCM, "the harmony between the body and mind" and "cultivating both body and mind" embody the holistic view of the principles of Chinese traditional culture. Psychosomatic medicine embodies its practical aesthetics with the idea of cultivating both body and mind and the medical practice, which advocates the beliefs of respecting benevolence and morality, upholding harmony and emphasizing education in traditional Chinese culture. This paper expounds the practical aesthetics of psychosomatic medicine advocating the cultivation of both the body and mind. Firstly, the accumulation of qi forms the body; following the body? shen (mind) generates. The relationship between the body and mind is maintained by benevolence", which is the general rule to do a deed. The medical practice of psychosomatic medicine, advocating u the harmony between the body and mind" and "cultivating both body and mind", embodies the relevance between the body and mind, Secondly, Nourishing shen (mind) requires "keeping a tranquilized mind", which should be sought in "respect" and cultivating both body and mind. Thirdly, gezhi (studying the nature of phenomena), as one of the carriers of the principles of traditional Chinese culture, guides the understanding of all procedures in TCM diagnosis and treatment by emphasizing "the harmony between the body and mind" and "cultivating both body and mind"- The TCM medical practice, under the guidance of gezhi, reflects the natural beauty of human beings? and the beauty of human nature with physical and mental health, which advocates human relations, respects benevolence and morality, advocates harmony and emphasizes education. [ABSTRACT FROM AUTHOR]

【Objective】 To screen the optimal hormone treatment method for polychromatic crape myrtle cuttings for improving the production efficiency of polychromatic crape myrtle.【Methods】 The young branches of polychromatic crape myrtle were used as cuttings to analyze rooting rate and root effect index under different hormone types,hormone concentrations,treatment time,treatment part,and to detect its endogenous hormones and nutrients changes during the rooting process of cuttings under the optimal treatment and water soaking.【Results】 The hormone treatment could improve polychromatic crape myrtle rooting significantly. Hormone types and hormone concentration both had a highly significant effect on the rooting rate and root effect index（P<0.05）,and treatment time could affect significantly the rooting rate only （P<0.05), but reatment part had no significant difference （P>0.05）. The comprehensive influence of four factors was hormone types>hormone concentrations>treatment time>treatment part. The optimal treatment was 400 mg/L IBA to treat the lower 1/3 part of the branches for 5 min. The detection of endogenous hormones and nutrients during the rooting process of cuttings that were optimally treated and soaked in water showed that hormone could increase the content of IAA, soluble sugar content and soluble protein content in the critical period of rooting,meanwhile reduce the ABA and ZR content,and promote differentiation of root primordia and elongation of adventitious roots.【Conclusion】 The cutting effect of 400 mg/L IBA on the lower 1/3 part of branches for 5 min was better, and the higher rooting rate and root effect index of polychromatic crape myrtle could be obtained. [ABSTRACT FROM AUTHOR]

Objective: To explore the influencing factors of mental resilience in patients with lung cancer, and to analyze the relationship between mental resilience, social support and quality of life in patients with lung cancer. Methods: A total of 173 patients with lung cancer (lung cancer group) admitted to our hospital from June 2018 to December 2019 and 116 healthy patients (control group) who underwent physical examination in our hospital during the same period were selected. Connor -Davidson resilience scale (CD-RISC), Social Support Rating Scale (SSRS) and Health Status Survey Brief Form (SF-36) were used to assess mental resilience, social support, and quality of life of all subjects. The clinical data of patients were collected, the factors affecting mental resilience of patients with lung cancer were analyzed. Pearson test was used for correlation analysis. Results: THE CD-RISC, SSRS and SF-36 scores in the lung cancer group were lower than those in the control group (P<0.05). There were significant differences in CD-RISC scores among patients with different age, gender, education level, character characteristics, SSRS score, treatment stage, recurrence or metastasis (P<0.05). Multiple linear regression analysis showed that gender, education level, character characteristics, SSRS score, treatment stage, recurrence and metastasis were all influencing factors of mental resilience of patients with lung cancer (P<0.05). Pearson correlation analysis showed that CD-RISC scores were positively correlated with SSRS and SF-36 scores in patients with lung cancer (r=0.567, 0.617; P=0.047, 0.346). Conclusion: Mental resilience of patients with lung cancer is lower than that of normal people. Gender, education level, character characteristics, SSRS scores, treatment stage, recurrence and metastasis are the main factors affecting mental resilience of patients with lung cancer. Mental resilience of patients with lung cancer is related to social support and quality of life. [ABSTRACT FROM AUTHOR]

近年来面对新发突发传染病的相继流行,人们愈发认识到生物安全事关人民健康、国家安全与 长治久安,同时也对我国快速提高生物安全治理能力提出了要求。因此,培养微生物相关技术人才,提高其解 决实际问题的应用实践能力,对完善疾病预防控制体制和公共卫生体系十分重要。在生物安全防控方面,以新 型冠状病毒肺炎疫情为例,环境卫生学教学可在现有讲授式教学法为主的基础上,增加案例教学法、问题导向 学习法,开展科研形式的第二课堂,多种教学方法互相配合、层层推进,充分调动起学生学习主动性,能够显著 提高学生对微生物知识的掌握程度和实际应用效果。 [ABSTRACT FROM AUTHOR]

Objective To investigate the correlation of signal intensity on Gd-EOB-DTPA-enhanced magnetic resonance imaging(MRI) with the expression of organic anion transport polypeptide 1(OATP1) and multidrug resistance-associated protein 2(MRP2) on the surface of hepatocyte membrane.Methods A total of 16 healthy adult male beagle dogs were randomly divided into control group and acute liver failure group,with 8 dogs in each group.Gd-EOB-DTPA-enhanced MRI was performed for the dogs in the acute liver failure group at 24 hours after modeling to measure the signal intensity of different signal areas of liver parenchyma and the percentage of enhancement slope in the first-pass rapid rise period,and according to the MRI images of the hepatocyte phase(20 minutes after the injection of Gd-EOB-DTPA),the high-,medium-,and low-signal areas were selected for liver biopsy.Gd-EOB-DTPA-enhanced MRI was performed for the dogs in the control group after the injection of an equal volume of normal saline using the method,and liver biopsy was performed in the corresponding area.Western blot was used to measure the expression of the Gd-EOB-DTPA uptake protein OATP1 and its excretion protein MRP2 in liver tissue.An analysis of variance was used for comparison of continuous data between multiple groups,and the least significant difference t-test was used for further comparison between two groups;the independent samples t-test was used for comparison of the protein expression of OATP1 and MRP2 in the same signal area between groups.A Pearson correlation analysis was used to investigate the correlation between protein expression levels and signal intensity.Results The Gd-EOB-DTPA-enhanced MRI images of the beagle dogs with acute liver failure showed a significant difference in signal between the high-,medium-,and low-signal areas during hepatocyte phase,and there were also significant differences in the corresponding SIbase value,SIst value,and percentage of enhancement slope during the first-pass rapid rise period(F=70.70,55.12,and 19.82,all P<0.001).In the acute liver failure group,there were significant differences in the expression of OATP1 and MRP2 in liver tissue between different signal areas(F=7.508 and 6.212,both P<0.01),and compared with the control group,the acute liver failure group had a significant reduction in the expression of OATP1 in the medium-and low-signal areas(t=3.301 and 3.641,both P<0.05),which was consistent with the trend of signal reduction in hepatocyte phase;the reduction in the low-signal area was significantly greater than that in the high-signal area(t=3.436,P<0.05) and the medium-signal area(t=2.378,P<0.05).Compared with the control group,the acute liver failure group had a significant increase in the expression of MRP2 in the high-,medium-,and low-signal areas,with a tendency to increase from the high-signal area to the medium-signal area and low-signal area(t=-7.236,-8.130,and-9.614,all P<0.05).Conclusion The expression levels of OATP1 and MRP2 on the surface of hepatocyte membrane are correlated with the degree of enhancement of liver parenchyma in the hepatocyte phase of Gd-EOB-DTPA-enhanced MRI,which provides a certain basis for the segmental assessment of hepatocyte function. [ABSTRACT FROM AUTHOR]

With the transformation and upgrading of the traditional integrated library systems, how to connect the old system and the new service to meet the demand of library development and reader service becomes a technical challenge. To explore the micro-service and FOLIO architecture, this article takes the queue management system of closed stacks of Peking University Library as object to introduce the design ideas, system architecture and technology usage. Besides proposing an improved method of library services based on analysis of the database operations of the system, this article also discusses the technical advantages, application scenarios of micro-service architecture and the changing of library roles under this architecture. Finally, it suggests that the micro-service applied on library service and management is innovative. [ABSTRACT FROM AUTHOR]

Objective To analyze the risk factors and predictors related to postoperative delirium(POD) in liver transplantation. Methods The clinical data of 232 liver transplant recipients were retrospectively analyzed. Recipients were assigned to POD group (n=60) and non-POD (n=172) group according to the presence of POD. The intra- and post-operative conditions were compared between the two groups of liver transplant recipients. The risk factors for occurrence of POD in liver transplant recipients were analyzed using multifactorial analysis. And the value of predicting the occurrence of POD in liver transplant recipients according to the risk factors were assessed. Results The incidence of POD in liver transplant recipients was 25.9%. The operation time and anhepatic phase in the POD group were longer than those in the non-POD group. Intraoperative infusion of erythrocyte, infusion of cryoprecipitate, and lactic acid level were higher than those in the non-POD group (all P<0.05). The levels of postoperative alanine aminotransferase (ALT), aspartate aminotransferase (AST), prothrombin time international normalized ratio (PT-INR), and plasma fibrinogen in the POD group were significantly higher than those in the non-POD group (all P<0.05). Preoperative hepatic encephalopathy, elevated blood ammonia, high score of model for end-stage liver disease (MELD), elevated postoperative AST level and long intraoperative anhepatic phase were the independent risk factors for POD in liver transplant recipients (all P<0.05). Preoperative elevated blood ammonia and high MELD score showed profound value in predicting the occurrence of POD in liver transplant recipients, with best cut-off values of 42.6 μmol/L and 18 points, sensitivity of 0.650 and 0.767 as well as specificity of 0.826 and 0.727, respectively. Conclusions The incidence of POD is high in liver transplant recipients. Preoperative hepatic encephalopathy, elevated blood ammonia, high MELD score, elevated postoperative AST level, and long intraoperative anhepatic phase are independent risk factors for liver transplant POD. Preoperative elevated blood ammonia and high MELD score are predictors of POD in transplant recipients. [ABSTRACT FROM AUTHOR]

The flood disaster risk management of the heritage sites is an important part of the regional and urban flood risk management system, and should be explored based on a sound flood risk management system. Through literature review and case study, it combed the flood risk management systems, flood risk analysis and assessment methods of typical countries such as the United Kingdom and the Netherlands in recent years, summarized the development trend such as top-down and multi-level management system, combination of engineering and non-engineering measures, post-disaster response and pre-disaster prevention. Through the cases of heritage sites, Dordrecht in the Netherlands and Venice in Italy and other cases, the importance, management and technical methods of flood risk management in heritage sites were demonstrated, especially through comprehensive planning, emergency management, risk analysis, and public participation. It could provide reference for the management of urban flood control and disaster mitigation, the construction of flood risk assessment system and the formulation of multi-level flood control measures for heritage sites in China. [ABSTRACT FROM AUTHOR]

Objective To evaluate the efficacy and safety of mechanical thrombectomy in over-70-year-old patients with acute ischemic stroke. Methods The data of 62 cases who underwent mechanical thrombectomy in the Second Affiliate of Bengbu Medical College from June 2015 to May 2019 due to acute ischemic cerebral infarction were analyzed retrospectively, including 30 cases ≥70 years old, and 32 cases ＜70 years old. The recanalization rate of the two groups patients, the improvement rate of the 24 h National Institutes Health Stroke Scale(NIHSS) score and the 90 d Modified Rankin Scale(mRS) score were analyzed and compared. Results 28 patients with successful revascularization in the over-70-year-old group, including 18 with complete recanalization, 12 with improved 24 h NIHSS score, and 15 with improved 90-day mRS score. In the below-70-year-old patient group, 30 patients underwent successful revascularization, including 17 with complete recanalization, 13 with 24 h NIHSS score improvement, and 21 with improved 90-day mRS score. Statistical analysis showed that there was no significant difference between the two groups in the successful revascularization rate, complete recanalization rate and 90-day mRS score improvement rate(all P＞0.05). Regression analysis showed that baseline NIHSS score and complete revascularization were important indicators of 90-day prognosis. Conclusion The main outcomes of patients who underwent mechanical thrombectomy are baseline NIHSS scores and complete revascularization rather than age classification. [ABSTRACT FROM AUTHOR]

The current OpenIE methods cannot take into account the compactness of the extraction results and the performance of the model at the same time, which makes the extraction results unable to be better applied to downstream tasks. Therefore, this paper proposed a model that used biaffine attention for table filling and iterative extraction. Firstly, the model learned the directional information between words through biaffine attention, captured the interaction between word pairs, and then filled the two-dimensional table to make the components in the sentence share each other and identify compact components. Secondly, it used the multi-head attention mechanism to apply the representation of predicates and parameters to the context embedding, making the extraction of predicates and parameters dependent on each other and better linking the relationship components and parameter components. Finally, for sentences containing multiple relational components, it used iterative extraction to capture the inherent dependencies between each extraction without recoding. Experiments on the public datasets CaRB and Wire57 show that this method achieves higher precision and recall than baseline methods, improving F₁ values by at least 1.4% and 3.2%, while producing shorter and semantically richer extractions. [ABSTRACT FROM AUTHOR]

In order to explore the influencing factors of aerobic capacity of male students in Beijing colleges and universities, 134 male students aged 18 ~ 25 years old in Beijing colleges and universities were selected by random sampling method, and venous blood was drawn in fasting to measure the blood indexes, and gas metabolism was monitored in real time with the MetaMax 3B system from Germany, and the relative maximum oxygen uptake (VO2max) was measured by a linear incremental scheme. The results were analyzed and processed based on Spearman􀆳s correlation and ordered Logistic regression. The results show that the factors affecting the aerobic capacity of male students in Beijing colleges and universities in the regression equation are body weight (M), heart rate (HR), stroke volume (SV), ventricular ejection time (VET) and hemoglobin (HGB). The P of the combined regression equation model coefficients test step, block, and model tests are less than 0. 01. The - 2 log likelihood value ( - 2LL) of the goodness-of-fit test is 159. 374, the Cox & Snell R 2 is 0. 331, and the Nagelkerke R 2 is 0. 373. The predicted rank 1 accuracy is 45. 5%, rank 2 accuracy is 100%, rank 3 accuracy is 100%, and the combined is 81. 8%, indicating that the Logistic regression model performs well. Hosmer and Lemeshow test predicts that there is no significant difference between the predicted values and the observed values (P > 0. 05). This indicates that the multivariate Logistic regression model of quantitative loading cardiac function, blood indexes and aerobic capacity of male students in Beijing colleges and universities has a good fit, and HR, SV, VET and HGB are important factors to predict aerobic capacity of male students in Beijing colleges and universities. Meanwhile, the subjects do not need to exercise to the limit state, and the intensity of exercise is greatly reduced, which can effectively avoid the occurrence of exercise risk, and the test results of the regression model are better and good, which is suitable for the promotion in a large sample of the population. [ABSTRACT FROM AUTHOR]

Cd contamination is serious in some Se-rich paddy soils, which has posed potential human health risks through soil-to-crop transfer of Cd. Being the staple food feeding more than half population of China, rice has exhibited a prominent capability of Cd uptake and accumulation. With arable land per capita being less than half of the world average, it is quite essential to develop strategies to deal with soil Cd contamination for food safety and agricultural sustainability in China. In order to achieve safe utilization of Se-rich paddy soil, it is essential to reduce Cd bioavailability and thus mitigate its accumulation in rice grain. Previous studies have showed that it is difficult to achieve effective mitigation on Cd transfer into paddy rice by using a single ameliorator. The present work studied the effect of different combination of three kinds of silicates, i.e. sepiolite(SP), silica-calcium composite mineral(CS) and water-soluble foliar silicon fertilizer(YS), on bioavailability of Cd and Se in paddy soils and their transport into rice. Firstly, the dynamic changes Cd/Se/Si/Ca in soil pore water were determined with sepiolite(SP), sepiolite-silicon calcium composite mineral(SPC) and sepiolite-silicon calcium composite mineral-water soluble silicon mineral fertilizer(SCY) treatments. Secondly, the Cd and Se concentration of brown rice was investigated to understand exactly the mitigating effect of each treatment on grain Cd accumulation. Finally, the impacts of Si treatments on the availability and uptake of some mineral nutrients were also identified and discussed. The results showed that with SP, SPC and SCY treatment, the pH of rice rhizosphere soil increased by 0.15-0.31 units, which largely favored Cd transformation from easily exchangeable pool to carbonate- and organic-bound fractions while Se from residual fraction to bioavailable pool. In parallel, the concentration of Si, Ca and Se in soil pore water was remarkably enhanced by SPC and SCY treatment. Compared to control(CK), CaCl2-Cd in rhizosphere treated by SP, SPC and SCY was decreased by 19.5%-34.0%, while available Se was enhanced by 17.8%-36.8%. Transport factor(TF stem/white root) of Cd in rice plants from SPC and SCY treatments decreased by 92.1%-96.5% relative to that of CK, suggesting a significantly enhanced Cd sequestration in rice root. Most importantly, Cd concentration in brown rice was decreased from 0.32 mg/kg in CK to 0.15 and 0.02 mg/kg with SPC and SCY amendment, respectively, which were 25% and 90% lower than Chinese food safety standards for total Cd in rice (0.2 mg/kg). Each treatment had no significant effect on the accumulation of Se and other essential mineral elements (Cu, Fe, Zn, Mg and Mn) in brown rice. Taken together, SCY exhibited the most prominent mitigation effect on Cd accumulation in rice grain, which provides a cost-effective pathway for safe utilization of Cd-rich paddy soils without affecting Se enrichment in food chain. [ABSTRACT FROM AUTHOR]

Ｔｏ ｉｎｖｅｓｔｉｇａｔｅ ｔｈｅ ｖａｌｕｅ ｏｆ Ｇｄ － ＥＯＢ － ＤＴＰＡ － ｅｎｈａｎｃｅｄ ＭＲＩ ｉｎ ｔｈｅ ａｓｓｅｓｓｍｅｎｔ ｏｆ ｌｉｖｅｒ ｆｕｎｃｔｉｏｎ ｉｎ ｄｏｇｓ ｗｉｔｈ ａｃｕｔｅ ｌｉｖｅｒ ｆａｉｌｕｒｅ （ＡＬＦ）． Ｍｅｔｈｏｄｓ Ａ ｔｏｔａｌ ｏｆ １６ ｈｅａｌｔｈｙ ａｄｕｌｔ ｍａｌｅ Ｂｅａｇｌｅ ｄｏｇｓ ｗｅｒｅ ｒａｎｄｏｍｌｙ ｄｉｖｉｄｅｄ ｉｎｔｏ ＡＬＦ ｇｒｏｕｐ ａｎｄ ｂｌａｎｋ ｃｏｎｔｒｏｌ ｇｒｏｕｐ，ｗｉｔｈ ８ ｄｏｇｓ ｉｎ ｅａｃｈ ｇｒｏｕｐ． Ｔｈｅ ｄｏｇｓ ｉｎ ｔｈｅ ＡＬＦ ｇｒｏｕｐ ｗｅｒｅ ｇｉｖｅｎ ｉｎｊｅｃｔｉｏｎ ｏｆ ３０％ ａｃｅｔａｍｉｎｏｐｈｅｎ ｖｉａ ｔｈｅ ｓａｐｈｅｎｏｕｓ ｖｅｉｎ ｏｆ ｔｈｅ ｆｏｒｅ ｌｉｍｂ ａｎｄ ａｂｄｏｍｉｎａｌ ｓｕｂｃｕｔａｎｅｏｕｓ ｉｎｊｅｃｔｉｏｎ ｏｆ ４０％ ｃａｒｂｏｎ ｔｅｔｒａｃｈｌｏｒｉｄｅ ａｔ ａ ｄｏｓｅ ｏｆ ０． ５ ｍｌ／ ｋｇ，ａｎｄ １２ ｈｏｕｒｓ ｌａｔｅｒ，３０％ ａｃｅｔａｍｉｎｏｐｈｅｎ ａｎｄ ４０％ ｃａｒｂｏｎ ｔｅｔｒａｃｈｌｏｒｉｄｅ ｗｅｒｅ ｉｎｊｅｃｔｅｄ ａｔ ｈａｌｆ ｏｆ ｔｈｅ ｆｉｒｓｔ ｄｏｓｅ． Ｇｄ － ＥＯＢ － ＤＴＰＡ － ｅｎｈａｎｃｅｄ ＭＲＩ ｓｃａｎ ｗａｓ ｐｅｒｆｏｒｍｅｄ ａｆｔｅｒ ２４ ｈｏｕｒｓ， ａｎｄ ａｆｔｅｒ ｉｎｊｅｃｔｉｏｎ ｏｆ ｔｈｅ ｃｏｎｔｒａｓｔ ａｇｅｎｔ Ｇｄ － ＥＯＢ － ＤＴＰＡ （０． １５ ｍｌ／ ｋｇ ｂｏｄｙ ｗｅｉｇｈｔ），ｔｈｅ ｓｉｇｎａｌ ｖａｌｕｅｓ ｏｆ ｈｉｇｈ － ，ｍｅｄｉｕｍ － ，ａｎｄ ｌｏｗ － ｓｉｇｎａｌ ｒｅｇｉｏｎｓ ｗｅｒｅ ｍｅａｓｕｒｅｄ ｄｕｒｉｎｇ ｐｌａｉｎ ｓｃａｎ ａｎｄ ａｔ ２０ ａｎｄ ２６ ｍｉｎｕｔｅｓ ｏｆ ＭＲＩ ｓｃａｎ． Ｌｉｖｅｒ ｐｕｎｃｔｕｒｅ ｗａｓ ｐｅｒｆｏｒｍｅｄ ｉｎ ｄｉｆｆｅｒｅｎｔ ｓｉｇｎａｌ ｒｅ ｇｉｏｎｓ． Ｇｄ － ＥＯＢ － ＤＴＰＡ － ｅｎｈａｎｃｅｄ ＭＲＩ ｗａｓ ｐｅｒｆｏｒｍｅｄ ｆｏｒ ｔｈｅ ｃｏｎｔｒｏｌ ｇｒｏｕｐ，ａｎｄ ｌｉｖｅｒ ｐｕｎｃｔｕｒｅ ｗａｓ ｐｅｒｆｏｒｍｅｄ ｉｎ ｔｈｅ ｃｏｒｒｅｓｐｏｎｄｉｎｇ ｒｅ ｇｉｏｎｓ． ＨＥ ｓｔａｉｎｉｎｇ ａｎｄ ｅｌｅｃｔｒｏｎ ｍｉｃｒｏｓｃｏｐｙ ｗｅｒｅ ｕｓｅｄ ｆｏｒ ｔｉｓｓｕｅ ｅｘａｍｉｎａｔｉｏｎ，ａｎｄ ｔｈｅ ｔｗｏ ｇｒｏｕｐｓ ｗｅｒｅ ｃｏｍｐａｒｅｄ ｉｎ ｔｅｒｍｓ ｏｆ ｓｉｇｎａｌ ｖａｌｕｅｓ ａｎｄ ｔｈｅ ｒｅｓｕｌｔｓ ｏｆ ＨＥ ｓｔａｉｎｉｎｇ ａｎｄ ｅｌｅｃｔｒｏｎ ｍｉｃｒｏｓｃｏｐｙ． Ｔｈｅ ｉｎｄｅｐｅｎｄｅｎｔ ｓａｍｐｌｅｓ ｔ － ｔｅｓｔ ｗａｓ ｕｓｅｄ ｆｏｒ ｃｏｍｐａｒｉｓｏｎ ｏｆ ｃｏｎｔｉｎｕｏｕｓ ｄａｔａ ａｔ ｅａｃｈ ｔｉｍｅ ｐｏｉｎｔ ｂｅｔｗｅｅｎ ｔｈｅ ｔｗｏ ｇｒｏｕｐｓ，ａｎｄ ａｎ ａｎａｌｙｓｉｓ ｏｆ ｖａｒｉａｎｃｅ ｗｉｔｈ ｒｅｐｅａｔｅｄ ｍｅａｓｕｒｅｓ ｗａｓ ｕｓｅｄ ｆｏｒ ｃｏｍｐａｒｉｓｏｎ ａｔ ｄｉｆｆｅｒｅｎｔ ｔｉｍｅ ｐｏｉｎｔｓ ｗｉｔｈｉｎ ｇｒｏｕｐｓ；ａ ｏｎｅ － ｗａｙ ａｎａｌｙｓｉｓ ｏｆ ｖａｒｉａｎｃｅ ｗａｓ ｕｓｅｄ ｆｏｒ ｃｏｍｐａｒｉｓｏｎ ｏｆ ｓｉｇｎａｌ ｖａｌｕｅｓ ａｔ ｅａｃｈ ｔｉｍｅ ｐｏｉｎｔ ｗｉｔｈｉｎ ｇｒｏｕｐｓ． Ｒｅｓｕｌｔｓ Ｔｈｅ ＡＬＦ ｇｒｏｕｐ ｈａｄ ｈｉｇｈ，ｍｅｄｉｕｍ，ａｎｄ ｌｏｗ ｓｉｇｎａｌｓ ｉｎ ｄｉｆｆｅｒｅｎｔ ｔｉｍｅ ｐｅｒｉｏｄｓ ａｆｔｅｒ Ｇｄ － ＥＯＢ － ＤＴＰＡ － ｅｎｈａｎｃｅｄ ＭＲＩ ｓｃａｎ，ａｎｄ ＨＥ ｓｔａｉｎｉｎｇ ａｎｄ ｅｌｅｃｔｒｏｎ ｍｉ ｃｒｏｓｃｏｐｙ ｓｈｏｗｅｄ ｖａｒｙｉｎｇ ｄｅｇｒｅｅｓ ｏｆ ｈｅｐａｔｏｃｙｔｅ ｉｎｊｕｒｙ ｉｎ ｔｈｅ ｈｉｇｈ － ，ｍｅｄｉｕｍ － ，ａｎｄ ｌｏｗ － ｓｉｇｎａｌ ｒｅｇｉｏｎｓ． Ｔｈｅ ＡＬＦ ｇｒｏｕｐ ｈａｄ ｓｉｇｎｉｆｉｃａｎｔ ｄｉｆｆｅｒｅｎｃｅｓ ｉｎ ｔｈｅ ｌｅｖｅｌｓ ｏｆ ａｌａｎｉｎｅ ａｍｉｎｏｔｒａｎｓｆｅｒａｓｅ，ａｓｐａｒｔａｔｅ ａｍｉｎｏｔｒａｎｓｆｅｒａｓｅ，ａｎｄ ｔｏｔａｌ ｂｉｌｉｒｕｂｉｎ ａｔ ０，６，１２，ａｎｄ ２４ ｈｏｕｒｓ （Ｆ ＝ ５４． ９０９， １４８ ２３８，ａｎｄ １１． ７７８，ａｌｌ Ｐ ＜０． ０１）． Ｉｎ ｔｈｅ ＡＬＦ ｇｒｏｕｐ ａｎｄ ｔｈｅ ｃｏｎｔｒｏｌ ｇｒｏｕｐ，ｔｈｅ ｈｉｇｈ － ，ｍｅｄｉｕｍ － ，ａｎｄ ｌｏｗ － ｓｉｇｎａｌ ｖａｌｕｅｓ ｓｈｏｗｅｄ ｓｉｇｎｉｆｉ ｃａｎｔ ｃｈａｎｇｅｓ ｄｕｒｉｎｇ ｐｌａｉｎ ｓｃａｎ ａｎｄ ａｔ ２０ ａｎｄ ２６ ｍｉｎｕｔｅｓ ｏｆ ＭＲＩ ｓｃａｎ （ＡＬＦ ｇｒｏｕｐ：Ｆ ＝ ２３２． ６９，６５． ３９，ａｎｄ ２４． ９１，ａｌｌ Ｐ ＜ ０ ０１；ｃｏｎｔｒｏｌ ｇｒｏｕｐ：Ｆ ＝８００． ４３，８１７． ３５，ａｎｄ ７９５． ７１，ａｌｌ Ｐ ＜０． ０１）． Ｉｎ ｔｈｅ ＡＬＦ ｇｒｏｕｐ，ｔｈｅｒｅ ｗｅｒｅ ｓｉｇｎｉｆｉｃａｎｔ ｄｉｆｆｅｒｅｎｃｅｓ ｉｎ ｔｈｅ ｈｉｇｈ － ，ｍｅｄｉｕｍ － ，ａｎｄ ｌｏｗ － ｓｉｇｎａｌ ｖａｌｕｅｓ ｄｕｒｉｎｇ ｐｌａｉｎ ｓｃａｎ ａｎｄ ａｔ ２０ ａｎｄ ２６ ｍｉｎｕｔｅｓ ｏｆ ＭＲＩ ｓｃａｎ （Ｆ ＝３． ９１４，２４． ３４３，ａｎｄ ２２． ４２４，ａｌｌ Ｐ ＜０． ０５）． Ｔｈｅｒｅ ｗｅｒｅ ｓｉｇ ｎｉｆｉｃａｎｔ ｄｉｆｆｅｒｅｎｃｅｓ ｂｅｔｗｅｅｎ ｔｈｅ ｔｗｏ ｇｒｏｕｐｓ ｉｎ ｔｈｅ ｍｅｄｉｕｍ － ａｎｄ ｌｏｗ － ｓｉｇｎａｌ ｖａｌｕｅｓ ａｔ ２０ ｍｉｎｕｔｅｓ ｏｆ ＭＲＩ ｓｃａｎ （ｔ ＝ － ７． ８１３ ａｎｄ － ７． ４９２， ｂｏｔｈ Ｐ ＜０ ００１）ａｎｄ ｔｈｅ ｈｉｇｈ － ，ｍｅｄｉｕｍ － ，ａｎｄ ｌｏｗ － ｓｉｇｎａｌ ｖａｌｕｅｓ ａｔ ２６ ｍｉｎｕｔｅｓ ｏｆ ＭＲＩ ｓｃａｎ （ｔ ＝ －３． ０７５，－４． ７８５，ａｎｄ －６． ３２６，Ｐ ＝ ０ ００８，Ｐ ＜０． ００１，ａｎｄ Ｐ ＜０． ００１）． Ｔｈｅ ｒｅｄｕｃｔｉｏｎ ｉｎ ｃｏｒｒｅｓｐｏｎｄｉｎｇ ｓｉｇｎａｌｓ ｗａｓ ａｓｓｏｃｉａｔｅｄ ｗｉｔｈ ｔｈｅ ｄｅｇｒｅｅ ｏｆ ｈｅｐａｔｏｃｙｔｅ ｉｎｊｕｒｙ． Ｃｏｎｃｌｕｓｉｏｎ Ｇｄ － ＥＯＢ － ＤＴＰＡ － ｅｎｈａｎｃｅｄ ＭＲＩ ｓｃａｎ ｉｓ ｆｅａｓｉｂｌｅ ｆｏｒ ｔｈｅ ａｓｓｅｓｓｍｅｎｔ ｏｆ ｌｉｖｅｒ ｆｕｎｃｔｉｏｎ ｉｎ ＡＬＦ ｄｏｇｓ，ａｎｄ ｉｔ ｃａｎ ａｌｓｏ ｐｒｅｄｉｃｔ ｔｈｅ ｄｅｇｒｅｅ ｏｆ ｌｉｖｅｒ ｐａｔｈｏｌｏｇｉｃａｌ ｄａｍａｇｅ． [ABSTRACT FROM AUTHOR]

With the improvement of quality of life, the life expectancy of residents is generally prolonged, and people suffering from Alzheimer's disease (AD) is increasing. Epidemiological and animal experiments have found that atmospheric particulate matter is associated with AD. This article briefly reviews the mechanisms of AD-related oxidative stress damage and neuroinflammation caused by atmospheric fine particulate matter entering the brain via olfactory bulb pathway. [ABSTRACT FROM AUTHOR]

In shellfish culturing and breeding practice, the width of shell growth lines is an important index to measure the growth rate of shellfish, and anal angle radial rib is the part with the widest growth lines. In order to explore the feasibility of taking anal angle radial rib length as a typical growth trait, this research analyzed the correlation between anal angle radial rib length （X1） and other growth traits, such as shell length （X2）, shell height （X3）, total height （X4）, shell width （X5）, live body weight （Y1）, soft tissue weight （Y2） and shell weight （Y3） of Hyriopsis cumingii at different ages and conducted the path analysis of body size traits to body weight traits （live body weight, soft tissue weight and shell weight）. The results of correlation analysis showed that there was a significant positive correlation between anal angle radial rib length and body weight in the first to third instars of H. cumingii. The correlation coefficient between shell length and live body weight was the highest in the first and second instars of mussel, while the correlation coefficient between anal angle radiating rib length and live body weight was the highest in the third instar of mussel. In addition, the correlation coefficient between anal angle radiating rib length and live body weight, soft tissue weight and shell weight showed an increasing trend with the increase of age. The data of path analysis and multiple regression analysis displayed that the main factors affecting the live body weight of 1 to 3 year old were anal angle radial rib length, shell length and shell width, the main factors affecting the soft tissue weight of 3 year old were shell length and shell width, the main factors affecting the shell quality of 1 and 2 year old mussel were shell length and shell width, and the main factors affecting the shell quality of the third instar mussel were anal angle radiating rib length, shell length and shell width. In conclusion, anal angle radial rib length, together with shell length and shell width, can be used as a new typical growth trait to represent the growth rate of H. cumingii. [ABSTRACT FROM AUTHOR]

Hepatitis B virus(HBV)-specific T cells in patients with chronic HBV infection are currently acknowledged as exhausted T cells. This article summarizes previous studies which support this hypothesis and then points out several inconsistencies between this hypothesis and clinical observation. Based on recent research findings, it is pointed out that in patients with chronic HBV infection, HBV-specific T cells might be dominated by silenced, early-differentiated progenitor T cells. Also, this article analyzes the possible causes of the silenced status of those T cells. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects and mechanism of histone deacetylase inhibitor on the expression of multidrug resistance gene-1 (MDR1) and multidrug resistance-associated protein 1 (MRP1) in the lung cancer cells. Methods The mRNA expression of MDR1 and MRP1 in A549 and SK-mes-1 cells treated by SAHA and TSA were detected by RT-PCR. The protein expression of MDR1 and MRP1 in the A549 and SK-mes-1 cells treated by SAHA and TSA, and the expression of HDAC1, -2, -3, -4, and 2MeH3K9 in the A549 cells treated by SAHA and TSA was detected by Western blotting. Results SAHA and TSA increased the mRNA and protein expression of MDR1 but decreased the mRNA and protein expression of MRP1 in the A549 and SK-mes-1 cells (both P <0. 05). SAHA and TSA increased 2MeH3K9 expression and decreased HDAC3 and HD AC-4 expression in the A549 cells (both P <0. 05). Conclusion SAHA and TSA promote the MDR1 expression and inhibits the MRP1 expression in the lung cancer cells by simultaneously promoting the acetylation and methylation of histones. [ABSTRACT FROM AUTHOR]

Objective By observing the emission of particles and various gases under different combustion conditions during moxibustion, to further discuss the safety of moxibustion therapy. Methods Eighteen moxa sticks (1 g) from the same batch were put into three types of moxibustion devices and burnt out, which was detected by laser measuring dust and gas detectors. These moxa sticks were randomly divided into natural exposure group, moxa cylindrical device group and moxa cylindrical device with filter group (n = 6 in each group). Ignition was made in the sealed glass room,and PM2.5 (Particulate Matter 2.5) mass concentration, CO,SO2 and NO2 emissions were constantly monitored, and peak values of each one were noted. Results There were statistic differences among the three groups in PM2.5,CO and SO2levels. The concentration of PM2.5and the emission of CO and SO2 in group of moxa cylindrical device with filter were the lowest in the three groups (P < 0. 05). Moxibustion did not affect the content of NO2. The peak concentration of PM2.5 in descending order was ： moxibustion cylindrical device group > natural exposure group > moxibustion cylindrical device with filter group. The peak emission values of CO and SO2： natural exposure group > moxibustion cylindrical device group > moxibustion cylindrical device with filter group. Conclusion There were differences in the emission of PM2.5,CO and SO2 in different devices. Controlling the moxa burning conditions can reduce the emission of PM2.5, CO and SO2. Therefore physical barrier measures should be taken to effectively reduce the concentration of PM2.5 in moxibustion sites and avoid moxa smoke. [ABSTRACT FROM AUTHOR]

In order to understand the influence law of pressure pumping ratio β and the distance between air curtain generator and workface Lw on the air curtain dust resistance effect, the transport conditions of air and dust flow field under different β and i w coupling conditions were simulated by Fluent. The results show that the radial vortex air curtain turns to axial dust resistance air curtain gradually as β become smaller and Lw become larger. The bigger the β is and the larger the Lw is, the worse the dust resistance effect of radial vortex air curtain is and the larger the dust diffusion distance Ld become. The smaller the β is and the larger the Lw is, the steadier the axial dust resistance air curtain is, the smaller the Ld become, the dust resistance ability of axial dust resistance air curtain is better than that of radial vortex air curtain. According to the simulation results and actual production, the best ventilation parameter settings for air curtain dust resistance are as (3 = 0. 75 and Lw =20 m. These parameters are applied in the belt roadway of east 5th mining area of Huipodi Coal Mine. The relative errors of speed direction and magnitude between measured data and simulation results are small. It means that the simulation results are accurate. The axial dust resistance air curtain whose thickness is 3 m was formed in the workface, a high concentration dust could be controlled within the range in front of heading machine driver's section about 6 m from the workface, the dust resistance effect is obvious. [ABSTRACT FROM AUTHOR]

The experiment was conducted using 0.81 g Litopenaeus vannamei post-larvae as the subjects to study carbohydrate to lipid ratio in the feed on the growth, physiological and biochemhe effects of differentical indices, and body composition of Litopenaeus vannamei under a salinity of 30 mg/kg. Six isonitrogenous and isoenergetic feeds were prepared, with a protein level of 41% and carbohydrate-lipid ratios of 4.01∶1, 3.29∶1, 2.78∶1, 2.21∶1, 1.75∶1, and 1.42∶1, respectively. Each group had 3 replicates, with 30 shrimps in each replicate. The results showed that: the survival rate of Litopenaeus vannamei fed with a carbohydrate-lipid ratio of 3.29∶1 was significantly higher than those fed with ratios of 2.21∶ 1 and 1.42∶ 1 (P<0.05). The specific growth rate of Litopenaeus vannamei fed with a carbohydrate-lipid ratio of 2.78∶ 1 was significantly higher than those fed with ratios of 4.01∶1 and 3.29∶ 1 (P<0.05). The amylase activity of Litopenaeus vannamei fed with a carbohydratelipid ratio of 4.01∶ 1 was significantly higher than those fed with ratios of 1.75∶1 and 1.42∶1 (P<0.05). The hexokinase activity of Litopenaeus vannamei fed with a carbohydrate-lipid ratio of 4.01∶1 was significantly higher than the other groups. The liver glycogen content of Litopenaeus vannamei fed with a carbohydrate-lipid ratio of 3.29∶1 was significantly higher than those fed with a ratio of 1.42∶1 (P<0.05). The blood protein concentration of Litopenaeus vannamei fed with a carbohydrate-lipid ratio of 1.75∶1 was significantly higher than those fed with ratios of 1.42∶ 1 (P<0.05). The blood glucose content of Litopenaeus vannamei fed with a carbohydrate-lipid ratio of 4.01∶1 was significantly higher than those fed with a ratio of 1.42∶1 (P< 0.05). The cholesterol content of Litopenaeus vannamei fed with a carbohydrate-lipid ratio of 1.42∶1 was significantly higher than those fed with ratios of 4.01∶1, 3.29∶1, 2.78∶1, and 2.21∶1 (P<0.05). The triacylglycerol content of Litopenaeus vannamei fed with a carbohydrate-lipid ratio of 1.42∶1 was significantly higher than those fed with a ratio of 4.01∶1 and 2.21∶1 (P<0.05). Moreover, the crude fat content of Litopenaeus vannamei fed with carbohydrate to lipid ratio of 1.42∶1 was significantly higher than those fed with ratios of 4.01∶1, 3.29∶1, and 2.78∶1 (P<0.05). The results of the experiment showed that the optimal starch and fat requirements for Litopenaeus vannamei were 18.97% and 6.83%, respectively, and the optimal carbohydrate to lipid ratio was 2.78∶1. [ABSTRACT FROM AUTHOR]

Osteoporosis is a common clinical disease of bone metabolism associated with aging, and its prevalence increases with age. The primary cause of this disease is the imbalance of bone metabolism due to insufficient bone formation to offset the excessive bone resorption. Osteogenic differentiation of bone marrow mesenchymal stem cells is a key process in maintaining bone formation, and the decline in osteogenic differentiation ability of bone marrow mesenchymal stem cells caused by senescence plays a vital role in the pathological mechanism of osteoporosis. In this light, the present article summarizes the senescence mechanism of bone marrow mesenchymal stem cells and its relationship with osteoporosis, and intends to provide a new perspective for the prevention and treatment of osteoporosis. [ABSTRACT FROM AUTHOR]

Aiming at the situation of the same unit of supervision and management and unclear the rights and responsibilities in the traditional construction management mode of the Yellow River system at city and county levels, during the “13th Five-Year Plan” period, the Yellow River Jiaozuo Bureau took the lead in separating the Construction Management Department from Engineering Management Department for independent operation at city-level river affairs bureaus, entrusting the work of land requisition and removal of resettlement to local governments and carrying out the management mode of “government leadership, hierarchical responsibility, county based and project legal person participation”. Through improving mechanism, standardizing management and strengthening measures, it had effectively ensured the smooth progress of flood control project construction in Jiaozuo reach of the Yellow River and achieved good results in practical work. [ABSTRACT FROM AUTHOR]

As a new configuration tilt rotor aircraft, the variable diameter tiltrotor aircraft can provide design basis for the selection of variable diameter plan in the design stage according to the simulation results of its flight characteristics. In this paper, the flight performance calculation model and nonlinear flight dynamics model of variable diameter tiltrotor aircraft are established, and the prototype is simulated. According to the parameter influence on flight performance and trim results, the rotor diameter variation range, diameter variation timing and diameter variation control strategy of the numerical example prototype are planned. Finally, the best rotor diameter variation range of the prototype is 0.7R—1.15R. The best time to change the diameter is to complete the process of rotor diameter increase when flying forward in 360 km/h fixed wing mode, and complete the process of rotor diameter decrease when flying forward in 250 km/h fixed wing mode. At the same time, a variable cross-section twist tube type variable diameter rotor control strategy is designed based on Sikovsky variable diameter rotor. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of long non⁃coding RNA (lncRNA) LOC730101 on the biological behavior of hepatocellular carcinoma (HCC). Methods R software was used to analyze the difference in the expression of LOC730101 in 374 HCC tissues and 50 normal tissues from the LIHC dataset of TCGA. 40 pairs of HCC tissues and corresponding adjacent tissues were collected from the pa⁃ tients with HCC who underwent surgical resection and were pathologically diagnosed in Guangxi Medical University Cancer Hospital in 2018, and the HCC cell lines Huh ⁃7, HCCLM3, HepG2 and human normal liver cell line L02 were cultured. The relative expression level of LOC730101 in tissues and cell lines was detected by qRT⁃PCR. The stable overexpressing cell lines LOC730101 (OE⁃LOC730101) and knocking down LOC730101 (sh⁃LOC730101) were constructed by lentivirus infection in HCC cell lines Huh⁃7 and HCCLM3, the corresponding control group (control) and negative control groups (sh⁃NC, OE ⁃NC) was set up at the same time, and qRT ⁃PCR was used to verify the infection effect. The proliferation ability of cells in each group was detected by CCK ⁃8. The migration and invasion ability of cells in each group were detected by wound healing assay and Transwell invasion assay. Results TCGA database analysis showed that the expression level of LOC730101 in HCC tissues was higher than that in normal tissues (P<0.001). The results of qRT⁃PCR showed that the expression level of LOC730101 in HCC tissues was significantly higher than that in adjacent tissues (P<0.001). The expression level of LOC730101 in liver cancer cell lines Huh⁃7, HepG2 and HCCLM3 were also higher than that in human normal liver cell line L02 (all P<0.05 ). Compared with the corresponding negative control group, the proliferation, migration and invasion ability of cells were decreased in the sh ⁃LOC730101 group (all P<0.05), while increased in the OE ⁃LOC730101 group (all P<0.01). Conclu⁃ sions lncRNA LOC730101 is up⁃regulated in HCC tissues and cells, and may promote its proliferation, migration and invasion. [ABSTRACT FROM AUTHOR]

Objective: Discuss the effect of catalpol on brain-derived neurotrophic factor (BDNF) expression in 茁-Amyloid peptide (A茁) intoxicated SH-SY5Y cells and the mechanism of regulating BDNF expression. Methods: The SH-SY5Y neuroblastoma cells induced by all-trans retinoic acid were used as the research object, and the effect of catalpol on A茁-injured cells was studied. The cell survival rate was determined by MTT assay, and the content of BDNF was determined by ELISA. The Western blot was used to detect the expression levels of transcription factors cAMP response element binding protein (CREB) and phosphorylated CREB (pCREB, activated form of CREB). The mRNA expression levels of BDNF and its series of transcripts were determined by RT-PCR. CREB expression was blocked by RNA interference and the expression of BDNF mRNA was determined by RT-PCR. Results Catalpol pre-protection increased the survival rate of SH-SY5Y cells after A茁 injury, significantly increased the content of BDNF in cell culture supernatant and intracellular BDNF mRNA level, promoted the expression of BDNF transcription factor IV and its key regulatory transcription factor pCREB. After interfered with CREB expression, the upregulation effect of catalpol on BDNF mRNA expression partially disappeared. Conclusion Catalpol may play a neuroprotective role by upregulating the phosphorylation of CREB and promoting the expression of BDNF. [ABSTRACT FROM AUTHOR]

目的 对国内外发表的关于“互联网+”医疗服务项目的定价及支付准入的文献进行系统综述, 为我国 “互联网+” 医疗服务项目的定价及支付提供依据。方法 计算机检索 PubMed、Embase、Cochrane Library、中国知网、万方数据、中国生物医学文献数据库, 搜集 “互联网+” 医疗服务项目定价及支付准入相关文献资料, 检索时限为建库至 2020 年 11 月 30 日。由 2 名研究者独立筛选文献、提取资料, 进行描述分析。结果 共纳入16 项研究。“互联网+” 定价项目类型包括实时、电话及影像咨询等 3 种形式。项目涉及诊断、治疗、监测等多种服务及多个学科。支付定价的关键要素包括时间、服务形式、服务强度、应用场景、服务区域及专科类型等, 线上医师技术劳务支付标准与线下基本相同。结论 我国 “互联网+” 项目仍需进一步拓展, 需要进一步细化定价分层颗粒度, 探索创新支付方式, 以促进 “互联网+” 医疗服务的发展。 [ABSTRACT FROM AUTHOR]