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Reports on the structure of adenosine triphosphate (ATP):corrinoid adenosyltransferase from Salmonella typhimurium in its substrate-free state, complexed with MgATP or with hydroxocobalamin and MgATP. Formation of the cobalt-carbon bond; MgATP and hydroxocobalamin binding sites; Interactions between the protein and polar side chains of corrin ring.

Reports on the isolation of a cob(II)alamin reductase activity in Salmonella enterica serovar Typhimurium LT2 to homogeneity. Identification of NAD(P)H:flavin oxidoreductase (Fre) as the enzyme responsible for the activity; Inability of Fre to cause the generation of cob(I)alamin in vivo.