1. Protein Analysis by Shotgun/Bottom-up Proteomics.
- Author
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Yaoyang Zhang, Fonslow, Bryan R., Bing Shan, Baek, Moon-Chang, and Yates III, John R.
- Subjects
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PROTEOMICS , *PROTEIN analysis , *TWO-dimensional electrophoresis , *POLYACRYLAMIDE gel electrophoresis , *CHROMATOGRAPHIC analysis , *POST-translational modification , *MASS spectrometry - Abstract
The article discusses the technique of shotgun/ bottom-up proteomics for the analysis of protein. It states that bottom-up process characterizes protein by analyzing the peptides released from the protein through proteolysis. It discusses several techniques related to shotgun proteomics which include two dimensional polyacrylamide gel electrophoresis (20-PAGE), chromatographic methods and post-translational modification (PTM) analysis by Mass Spectrometry.
- Published
- 2013
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