1. Evaluation of Anticancer Activity of Nucleoside–Nitric Oxide Photo-Donor Hybrids.
- Author
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Marchesi, Elena, Melloni, Elisabetta, Casciano, Fabio, Pozza, Elena, Argazzi, Roberto, De Risi, Carmela, Preti, Lorenzo, Perrone, Daniela, and Navacchia, Maria Luisa
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CLICK chemistry , *CELL lines , *ANTINEOPLASTIC agents , *CELL survival , *NITRIC oxide - Abstract
Herein, we report the synthesis of a new hybrid compound based on a 2′-deoxyuridine nucleoside conjugated with a NO photo-donor moiety (dU-t-NO) via CuAAC click chemistry. Hybrid dU-t-NO, as well as two previously reported 2′-deoxyadenosine based hybrids (dAdo-S-NO and dAdo-t-NO), were evaluated for their cytotoxic and cytostatic activities in selected cancer cell lines. dAdo-S-NO and dAdo-t-NO hybrids displayed higher activity with respect to dU-t-NO. All hybrids showed effective release of NO in the micromolar range. The photochemical behavior of the newly reported hybrid, dU-t-NO, was studied in the RKO colon carcinoma cell line, whereas the dAdo-t-NO hybrid was tested in both colon carcinoma RKO and hepatocarcinoma Hep 3B2.1-7 cell lines to evaluate the potential effect of NO released upon irradiation on cell viability. A customized irradiation apparatus for in vitro experiments was also designed. [ABSTRACT FROM AUTHOR] more...
- Published
- 2024
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