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The experience of a cultural event presents, like any human activity, an intimate dimension. It is most often accompanied by emotions and the construction of knowledge. We have identified in particular a recurring relationship between the sense-making and emotions that suggests that knowing the emotions of visitors could enrich the reflection of museum designers. In order to gain an in-depth knowledge of the experience of audiences, we have implemented XEmotion, a new survey method for capturing emotions in a cultural context. This article explores the potentialities of such an approach which could become a new tool to apprehend a significant part of the public experience. We detail the background that led us to propose the XEmotion survey method as well as the evaluation of the uses of the charter itself by museum professionals. In the end, XEmotion seems to open new perspectives for analyzing the experience of the public. [ABSTRACT FROM AUTHOR]

Background: Immunosuppressive cell-based therapy is a recent strategy for controlling Graft-versus-Host Disease (GvHD). Such cells ought to maintain their suppressive function in inflammatory conditions and in the presence of immunosuppressive agents currently used in allogeneic hematopoietic cell transplantation (allo-HCT). Moreover, these therapies should not diminish the benefits of allo-HCT, the Graft-versus-Leukemia (GvL) effect. We have previously reported on a novel subset of human monocyte-derived suppressor cells (HuMoSC) as a prospective approach for controlling GvHD. Objective The objective of this study was to explore the therapeutic relevance of the HuMoSC in clinical conditions. Methods: Immune regulatory functions of HuMoSC were assessed in inflammatory conditions and in the presence of immunosuppressants. The therapeutic efficiency of the association of HuMoSC with immunosuppressants was evaluated in an experimental model of GvHD induced by human PBMC in NOD/SCID/IL2-Rγc-/- (NSG) mice.Interestingly, the inhibitory functions of HuMoSC against T lymphocytes and their ability to polarize Treg are preserved, in vitro, in inflammatory environments and are not affected by immunosuppressive agents. In vivo, the association of HuMoSC-based treatment with an immunosuppressive drug showed a synergistic effect for controlling GvHD. Furthermore, HuMoSC control GvHD while preserving GvL effect in a xeno-GvHD conditioned mouse model with cell neoplasm (CAL-1). HuMoSC are generated according to good manufacturing practices (GMP) and we demonstrated that these cells tolerate long-term preservation with unaltered phenotype and function. Conclusion HuMoSC-based therapy represents a promising approach for controlling GvHD and could be quickly implemented in clinical practice. [ABSTRACT FROM AUTHOR]

Since the 18th century, architects and museum managers have used natural and artificial lighting to light artworks and exhibits. Today, practitioners such as museographers, scenographers and lighting designers use it as a tool to build atmospheres and to guide the visitor's gaze. Beyond merely highlighting artwork, lighting defines what is shown, and indicates the object of discussion. The potential of lighting -- as a mediating device that directly affects the visitor's sensitive and cognitive experience -- remains to be shown and explained. It was from this standpoint that we studied visitor experience in the Grande Galerie de l'Évolution at the Muséum national d'Histoire naturelle in Paris, which presents a dynamic lighting installation conceived as an immersive scenography. We found that this installation plays an important role in the process of constructing sense and visitor experience. It helps to create an atmosphere of well-being, evoking the colors of natural environments, lighting or darkening the exhibits to attract or reduce attention, and acts as a catalyst for interpersonal and contemplative experiences. These results encourage us to explore the potential of lighting as a mediating device. [ABSTRACT FROM AUTHOR]

This research focuses on a new form of mediatization of testimonials. The Illinois Holocaust Museum and Education Center (Skokie, United States) offers a "holographic theater" in which a surviving Holocaust witness can be interviewed. In this theater, everything happens as if the Great Witness were there in person, interviewed by a facilitator to answer questions from visitors. But the Great Witness is not there, only a recorded holographic projection appears with which visitors can converse. How can a program and an algorithm replace a human presence? Interviews with visitors are carried out in stimulated recall and allow us to understand the dynamics of an exceptional experience. [ABSTRACT FROM AUTHOR]

This study aimed to assess the implication of mucosal-associated invariant T (MAIT) cells in GCA. Blood samples were obtained from 34 GCA patients (before and after 3 months of treatment with glucocorticoids (GC) alone) and compared with 20 controls aged >50 years. MAIT cells, defined by a CD3+CD4−TCRγδ-TCRVα7.2+CD161+ phenotype, were analyzed by flow cytometry. After sorting, we assessed the ability of MAIT cells to proliferate and produce cytokines after stimulation with anti CD3/CD28 microbeads or IL-12 and IL-18. MAIT were stained in temporal artery biopsies (TAB) by confocal microscopy. MAIT cells were found in the arterial wall of positive TABs but was absent in negative TAB. MAIT frequency among total αβ-T cells was similar in the blood of patients and controls (0.52 vs. 0.57%; P = 0.43) and not modified after GC treatment (P = 0.82). Expression of IFN-γ was increased in MAIT cells from GCA patients compared to controls (44.49 vs. 32.9%; P = 0.029), and not modified after 3 months of GC therapy (P = 0.82). When they were stimulated with IL-12 and IL-18, MAIT from GCA patients produced very high levels of IFN-γ and displayed a stronger proliferation compared with MAIT from controls (proliferation index 3.39 vs. 1.4; P = 0.032). In GCA, the functional characteristics of MAIT cells are modified toward a pro-inflammatory phenotype and a stronger proliferation capability in response to IL-12 and IL-18, suggesting that MAIT might play a role in GCA pathogenesis. Our results support the use of treatments targeting IL-12/IL-18 to inhibit the IFN-γ pathway in GCA. • MAIT cells are present in lesions of Giant Cell Arteritis (GCA). • Their functional characteristics are modified toward a pro-inflammatory phenotype. • They display a stronger proliferation capability in response to IL-12 and IL-18 and produce higher level of IFN-γ. [ABSTRACT FROM AUTHOR]