16 results on '"Chen, Ying-Xu"'
Search Results
2. Recent advances in nanomaterial-based electrochemical and optical sensing platforms for microRNA assays.
- Author
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Wang, Yi-Han, He, Liu-Liu, Huang, Ke-Jing, Chen, Ying-Xu, Wang, Shu-Yu, Liu, Zhen-Hua, and Li, Dan
- Subjects
MICRORNA ,QUANTUM dots ,OPTICAL elements ,DEVELOPMENTAL biology - Abstract
MicroRNA (MiRNA) plays a crucial role in biological cells to enable assessment of a cancer's development stage. Increasing evidence has shown that the accurate and sensitive detection of miRNA holds the key toward correct disease diagnosis. However, some characteristics of miRNAs, such as their short chains, low concentration, and similar sequences, make it difficult to detect miRNA in biological samples. Nanomaterials usually have good optical, electronic, and mechanical properties and therefore provide new possibilities for improving the performance of miRNA assays. Many different sorts of nanomaterials, including metal nanomaterials, carbon nanomaterials, quantum dots, and transition-metal dichalcogenides, have been used to construct optical and electrochemical assays for miRNA and have shown attractive results. This review describes recent efforts in the application of nanomaterials as sensing elements in electrochemical and optical miRNA assays. The analytical figures of merit of various methods for the detection of miRNA are compared in the present article. The current capabilities, limitations, and future challenges in miRNA detection and analysis based on nanomaterials are also addressed. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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3. An electrochemical microRNA sensing platform based on tungsten diselenide nanosheets and competitive RNA–RNA hybridization.
- Author
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Chen, Ying-Xu, Zhang, Wen-Jing, Huang, Ke-Jing, Zheng, Mingbo, and Mao, Ya-Cen
- Subjects
ELECTROCHEMICAL sensors ,MICRORNA ,TUNGSTEN selenide ,NUCLEIC acid hybridization ,MEDICAL research - Abstract
In this work, we report an ultrasensitive electrochemical biosensor for microRNA-21 (miRNA-21) detection by using a competitive RNA–RNA hybridization configuration. A biotinylated miRNA of the self-same sequence with the target miRNA is mixed with the samples, and allowed competition with the target miRNA for a thiolated RNA probe immobilized onto a tungsten diselenide (WSe
2 ) nanosheet modified electrode. Thereafter the current response is obtained by forming the hybridized biotinylated miRNA with streptavidin–horseradish peroxidase (HRP) conjugates to catalyze the H2 O2 + hydroquinone (HQ) system. Benefiting from the high specific surface area of WSe2 nanosheets, the competitive hybridization configuration and the signal amplification of the H2 O2 + HQ detection system, the proposed assay exhibits a wide linear range of 0.0001–100 pM towards target miRNA with a detection limit of 0.06 fM (S/N = 3), and shows excellent discrimination ability for base-mismatched miRNA sequences. Therefore, the designed platform has promising prospects for the detection of miRNA in biomedical research and early clinical diagnosis. [ABSTRACT FROM AUTHOR]- Published
- 2017
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4. A layered tungsten disulfide/acetylene black composite based DNA biosensing platform coupled with hybridization chain reaction for signal amplification.
- Author
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Shuai, Hong-Lei, Huang, Ke-Jing, and Chen, Ying-Xu
- Abstract
A 2-dimensional tungsten disulfide–acetylene black (WS
2 –AB) composite is synthesized by a simple hydrothermal method to achieve excellent electrochemical properties for applications as a DNA biosensor. The biosensor is fabricated based on the Au nanoparticles (AuNPs) and WS2 –AB composite modified electrode, which subsequently is used to couple with a capture probe by an Au–S bond, then modified with target DNA, auxiliary DNA and bio-H1–bio-H2 (H1–H2) to perform hybridization chain reaction for signal amplification. Herein, two DNA hairpins H1 and H2 are opened by the recognition probe. The nicked double helices from hybridization chain reaction are used to immobilize horseradish peroxidase enzymes via biotin–avidin reaction, which produces signal-amplification detection of target DNA through the catalytic reaction of the hydrogenperoxide + hydroquinone system. Under optimum conditions, the as-prepared biosensor shows a good linear relationship between the current value and logarithm of the target DNA concentration ranging from 0.001 pM to 100 pM and a detection limit as low as 0.12 fM. Moreover, the fabricated biosensor exhibits good selectivity to differentiate the one-base mismatched DNA sequence. This work will open a pathway for ultrasensitive detection of other biorecognition events and gene-related diseases based on layered WS2 –AB and hybridization chain reaction. [ABSTRACT FROM AUTHOR]- Published
- 2016
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5. Fatty liver index correlates with non-alcoholic fatty liver disease, but not with newly diagnosed coronary artery atherosclerotic disease in Chinese patients.
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Zhao-Yan Jiang, Chen-Ying Xu, Xian-Xing Chang, Wei-Wei Li, Lu-Ying Sun, Xiao-Bo Yang, Li-Fen Yu, Jiang, Zhao-Yan, Xu, Chen-Ying, Chang, Xian-Xing, Li, Wei-Wei, Sun, Lu-Ying, Yang, Xiao-Bo, and Yu, Li-Fen
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FATTY liver ,FATTY degeneration ,LIVER diseases ,CORONARY arteries ,CARDIOVASCULAR diseases - Abstract
Background: Fatty liver index (FLI) was recently established to predict non-alcoholic fatty liver disease (NAFLD) in general population, which is known to be associated with coronary artery atherosclerotic disease (CAD).This study aims to investigate whether FLI correlates with NAFLD and with newly diagnosed CAD in a special Chinese population who underwent coronary angiography.Methods: Patients with CAD (n = 231) and without CAD (n = 482) as confirmed by coronary angiography were included. Among them, 574 patients underwent B-ultrosonography were divided into NAFLD group (n = 209) and non-NAFLD group (n = 365). Correlation between FLI and NAFLD was analyzed using pearson's correlation. The associations between FLI and NAFLD as well as CAD were assessed using logistic regression. The predictive accuracy of FLI for NAFLD was evaluated using receiver operating characteristics (ROC) curve analysis.Results: FLI was significantly higher in NAFLD group (37.10 ± 1.95) than in non-NAFLD group (17.70 ± 1.04), P < 0.01. FLI correlated with NAFLD (r = 0.372, P < 0.001). The algorithm for FLI had a ROC-AUC of 0.721 (95% CI: 0.678-0.764) in the prediction of NAFLD. Logistic regression analysis showed that FLI was associated with NAFLD (adjusted OR = 1.038, 95% CI: 1.029-1.047, P < 0.01). The proportion of patients with CAD did not differ among the groups of FLI ≤ 30 (32.3%), 30-60 (31.0%), and ≥60 (35.3%). No significant association was found between FLI and CAD (adjusted OR = 0.992, 95% CI: 0.981-1.003 in men and OR = 0.987, 95% CI: 0.963-1.012 in women, P > 0.05).Conclusions: FLI showed good correlation with NAFLD in patients who underwent coronary angiography, but not with newly diagnosed CAD. This might be underestimated because some patients in non-CAD group may have other underlying cardiovascular diseases. [ABSTRACT FROM AUTHOR]- Published
- 2013
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6. Fertilization increases paddy soil organic carbon density.
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Wang, Shao-xian, Liang, Xin-qiang, Luo, Qi-xiang, Fan, Fang, Chen, Ying-xu, Li, Zu-zhang, Sun, Huo-xi, Dai, Tian-fang, Wan, Jun-nan, and Li, Xiao-jun
- Abstract
Field experiments provide an opportunity to study the effects of fertilization on soil organic carbon (SOC) sequestration. We sampled soils from a long-term (25 years) paddy experiment in subtropical China. The experiment included eight treatments: (1) check, (2) PK, (3) NP, (4) NK, (5) NPK, (6) 7F:3M (N, P, K inorganic fertilizers+30% organic N), (7) 5F:5M (N, P, K inorganic fertilizers+50% organic N), (8) 3F:7M (N, P, K inorganic fertilizers+70% organic N). Fertilization increased SOC content in the plow layers compared to the non-fertilized check treatment. The SOC density in the top 100 cm of soil ranged from 73.12 to 91.36 Mg/ha. The SOC densities of all fertilizer treatments were greater than that of the check. Those treatments that combined inorganic fertilizers and organic amendments had greater SOC densities than those receiving only inorganic fertilizers. The SOC density was closely correlated to the sum of the soil carbon converted from organic amendments and rice residues. Carbon sequestration in paddy soils could be achieved by balanced and combined fertilization. Fertilization combining both inorganic fertilizers and organic amendments is an effective sustainable practice to sequestrate SOC. [ABSTRACT FROM AUTHOR]
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- 2012
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7. The pathway of in-situ ammonium removal from aerated municipal solid waste bioreactor: nitrification/denitrification or air stripping?
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Hao, Yong-Jun, Min Ji, Chen, Ying-Xu, Wu, Wei-Xiang, Zhang, Shu-Guang, and Liu, Han-Qiao
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BIOREACTOR landfills ,SOLID waste ,AMMONIUM ,NITRIFICATION ,AIR stripping process (Water purification) ,DENITRIFICATION ,ACTIVATED sludge process ,AMMONIA & the environment - Abstract
In-situ ammonium removal from municipal solid waste (MSW) landfill is an attractive method due to its economic advantages. In this study, two simulated MSW bioreactors with different degrees of initial bio-stabilization were utilized to investigate the effects of intermittent aeration mode and the addition of activated sludge on the removal of ammonium. The results showed that up to 90% of ammonium could be removed and the amount of NOx—N produced was less than 1% of NH4+-N removed in both reactors. The pH values increased rapidly and finally arrived at a high level of 8.5—8.8. The efficiency of ammonium removal was improved by increasing the continuous aeration time, but it was not affected by the addition of activated sludge. A portion of liquid escaped from the reactors in the form of vapour, and as high as 195—258 mg L—1 of NH4 +-N was detected in the vapour collector. According to calculation, nitrification was inhibited by the high level of free ammonia in the bioreactors. As a result, air stripping was enhanced and became the primary pathway of ammonium removal. Therefore, controlling free ammonia concentration was essential in ammonium removal from the aerated MSW bioreactor. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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8. Effects of 60-day NO2 fumigation on growth, oxidative stress and antioxidative response in Cinnamomum camphora seedlings.
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Chen, Zhuo-mei, Chen, Ying-xu, Du, Guo-jian, Wu, Xi-lin, and Li, Feng
- Abstract
To study the oxidative stress and antioxidative response of Cinnamomum camphora seedlings exposed to nitrogen dioxide (NO
2 ) fumigation. Measurements were made up of the growth, chlorophyll content, chlorophyll fluorescence, antioxidant system and lipid peroxidation of one-year-old C. camphora seedlings exposed to NO2 (0.1, 0.5, and 4 μl/L) fumigation in open top chambers over a period of 60 d. After the first 30 d, 0.5 and 4.0 μl/L NO2 showed insignificant effects on the growth of C. camphora seedlings. However, exposure to 0.5 and 4.0 μl/L NO2 for 15 d significantly reduced their chlorophyll content ( P<0.05), enhanced their malondialdehyde (MDA) content and superoxide dismutase (SOD) activity ( P<0.05), and also significantly reduced the maximal quantum yield of PSII in the dark [the ratio of variable fluorescence to maximal fluorescence ( Fv / Fm )] ( P<0.05). In the latter 30 d, 0.5 μl/L NO2 showed a positive effect on the vitality of the seedlings, which was reflected by a recovery in the ratio of Fv / Fm and chlorophyll content, and obviously enhanced growth, SOD activity, ascorbate (AsA) content and glutathione reductase (GR) activity ( P<0.05); 4.0 μl/L NO2 then showed a negative effect, indicated by significant reductions in chlorophyll content and the ratio of Fv / Fm , and inhibited growth ( P<0.05). Conclusion: The results suggest adaptation of C. camphora seedlings to 60-d exposure to 0.1 and 0.5 μl/L NO2 , but not to 60-d exposure to 4.0 μl/L NO2 . C. camphora seedlings may protect themselves from injury by strengthening their antioxidant system in response to NO2 -induced oxidative stress. [ABSTRACT FROM AUTHOR]- Published
- 2010
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9. Environmental damage costs from fossil electricity generation in China, 2000∼2003.
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Zhang, Qing-yu, Wei, Yu-mei, Chen, Ying-xu, and Guo, Hui
- Abstract
Electricity consumption increases rapidly with the rapid development of China. The environmental damage costs of electricity generation are very important for both policy analysis and the proper management of the environment. A method was developed in this work to estimate gross environmental damage costs according to emission inventory and environmental cost factors, and to extend the costs from provincial to national level with population density. In this paper, sulfur dioxide (SO
2 ), nitrogen oxides (NOx ), particulate matter less than 10 μm in diameter (PM10 ), and carbon dioxide (CO2 ) from fossil fired power plants over 6000 kW were selected as index pollutants to quantify the environmental costs of damages on human health and global warming. With the new developed method, environmental damage costs, caused by 3 types of fired power plants in 30 provinces and 6 economic sectors during the years 2000 to 2003, were evaluated and analyzed. It can be seen that the calculated total national environmental damage costs of electricity have rapidly increased from 94930.87 × 106 USD in 2000 to about 141041.39 × 106 USD in 2003, with an average annual growth rate of 14.11%. Environmental damage costs of SO2 , NOx , PM10 , and CO2 are 69475.69 × 106 , 30079.29 × 106 , 28931.84 × 106 , and 12554.57 × 106 USD and account for 49.26%, 21.33%, 20.51%, and 8.90% of total environmental costs in fossil electricity generation, respectively. With regard to regional distribution, external costs caused by fossil electricity generation are mainly concentrated in the more populated and industrialized areas of China, i.e., the Eastern Central and Southeastern areas. [ABSTRACT FROM AUTHOR]- Published
- 2007
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10. Evaluation of organ distribution of microcystins in the freshwater phytoplanktivorous fish Hypophthalmichthys molitrix.
- Author
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Zhang, Hang-jun, Zhang, Jian-ying, Hong, Ye, and Chen, Ying-xu
- Abstract
To evaluate the public health risk of exposure to microcystins in fish food in China, the distribution pattern of microcystin-LR and microcystin-RR in various organs (liver, intestine, kidney, muscle and lipid) of the dominant freshwater phytoplanktivorous fish Hypophthalmichthys molitrix in Hangzhou, China’s Tiesha River was investigated with the method of HPLC-ESI-MS analysis. The distribution of microcystins was different in the fish organs and the major total microcystins (microcystin-LR and microcystin-RR) were present in the intestines (6.49 μg/g fresh weight), followed by the livers (4.52 μg/g fresh weight) and the muscles (2.86 μg/g fresh weight). Microcystins were detected in kidneys (1.35 μg/g fresh weight), but not detected in lipid. The results suggested that the mean daily intake from fish was 0.03 μg/kg body weight which was very close to the recommended WHO tolerable daily intake (TDI) level of 0.04 μg/kg body weight per day, and local people were warned they may have health risk if they consumed fish from the river. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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11. An investigation of cellular distribution of manganese in hyperaccumlator plant Phytolacca acinosa Roxb. using SRXRF analysis.
- Author
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Xu, Xiang-hua, Shi, Ji-yan, Chen, Ying-xu, Xue, Sheng-guo, Wu, Bei, and Huang, Yu-ying
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HYPERACCUMULATOR plants ,EFFECT of manganese on plants ,PHYTOREMEDIATION ,PHYTOLACCA ,MANGANESE in soils ,METABOLIC detoxification ,X-ray spectroscopy ,EPIDERMIS ,BIOACCUMULATION ,LOCALIZATION theory ,SYNCHROTRON radiation - Abstract
Phytolacca acinosa Roxb. (P. acinosa) is a recently discovered manganese hyperaccumulator plant from southern China. It is a good candidate for phytoremediation of manganese(Mn) polluted soil for its high biomass and fast growth. Knowledge of the tissue localization and identification of heavy metals can provide essential information on metal toxicity and bioaccumulation mechanisms. Synchrotron radiation X-ray fluorescence spectroscopy (SRXRF) microprobe was used in this study to investigate the cellular distributions of Mn and other elements in root, stem, leaf, petiole and midrib ofP. acinosa. The highest Mn content was found in the vascular tissues of root, stem, petiole and midrib. Cortex in root played a key role in Mn absorption and Mn was limited in the vascular bundle during the process of transportation in stem. Moreover, Mn content in leaf epidermis was higher than that in mesophyll, which suggested that the sequestration of Mn in leaf epidermis might be one of the detoxification mechanisms of P. acinosa. The significance of other elemental (such as P, S, K, Ca, Fe, Zn and Cu) distribution patterns and the correlation with Mn were also discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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12. Reaction site and mechanism in the UV or visible light induced TiO_2 photodegradation of Orange G.
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Yang Shi-ying, Lou Li-ping, Wu Xiao-na, and Chen Ying-xu
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TITANIUM dioxide ,OXIDES ,FLUORINATION ,CHEMICAL reactions ,HALOGENATION ,PHOTOCATALYSIS ,AZO dyes ,ENVIRONMENTAL protection ,ENVIRONMENTAL sciences - Abstract
For TiO_2 heterogeneous reaction, the reaction site and the detailed mechanism are interesting and controversy topics. In this paper, effects of surface fluorination of TiO_2 on the photocatalytic degradation of an azo dye, Orange G(OG) under UV or visible light irradiation were investigated, and the possible reaction site and mechanism were elucidated. The adsorption of OG on TiO_2 was nearly inhibited by fluoride but its UV light induced photodegradation rate was greatly increased by a factor of about 2.7, which was due to the more generated free hydroxyl radicals. It supported the views that fluoride could desorb the oxidant species from surface and that the reaction sites could move to the bulk solution. In TiO_2/Vis system, the observed inhibition effects of fluorination could be interpreted by the competitive adsorption, which provided additional evidences that the visible light sensitized photodegradation of dye pollutants on the catalyst surface. [ABSTRACT FROM AUTHOR]
- Published
- 2006
13. Microbial activity related to N cycling in the rhizosphere of maize stressed by heavy metals.
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Yang Ye, Chen Ying-xu, Tian Guang-ming, and Zhang Zi-jian
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PLANT-soil relationships ,PLANT roots ,NITRIFYING bacteria ,HEAVY metals ,PROKARYOTES ,FUNGUS-bacterium relationships - Abstract
A greenhouse experiment was carried out to compare differences in potential activities of ammonification, nitrification and denitrification in rhizosphere and bulk soil in a heavy-metal-stressed system. Exchangeable fractions of Cd, Cu and Cr were all higher in the rhizosphere of maize than in bulk soil. Results showed that the mineralization of N in soil was stimulated by low concentration of Cd. Addition of Cd at low levels stimulated the ammonifying and nitrifying activity in soil, while inhibitory influences were shown at high levels. Nitrifying bacteria was proved to be the most sensitive one, whilst the effect on denitrifying bacteria was very limited. Comparing Cd, Cu and Cr(VI) at 20 mg/kg soil, Cd was the most effective inhibitor of ammonification and denitrification, while Cr(VI) had the strongest inhibitory influence on nitrifying activity. Root exudates played important roles on the different exchangeable metal fractions and bacterial activities between rhizosphere and non-rhizosphere. Nitrate was the main form of mineral N in soil, as well as the main form of N absorbed by plants, but the formation and relative absorption of ammonium were promoted in response to high Cd exposure. [ABSTRACT FROM AUTHOR]
- Published
- 2005
14. Effect of organic fractions on sorption properties of organic pollutants in sediments.
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Chen Hua-lin, Zhou Jiang-min, Chen Ying-xu, and Xu Yun-tai
- Subjects
POLLUTANTS ,ORGANIC compounds ,HUMIC acid ,PHENANTHRENE ,LEACHING ,OXIDES - Abstract
Humic substrates are a major fraction of sediment organic matters, and the sorption of hydrophobic organic chemicals by humic substrates influences their behavior and fate in sediment. In this paper, organic matters were divided into non-humic substrates and humic substrates. Non-humic substrates include acid leaching fraction, acid extracted fraction, and lipid; humic substrates were fractionated into Ca-binding-FA (fulvic acid), Ca-binding-HA (humic acid), oxide-FA, oxide-HA, and humin. To study the effect of organic fractions on sorption properties, sorption kinetic and equilibrium sorption experiments of phenanthrene and pentachlorophenol (PCP) in five sediments were carried out. The results showed that the contents of acid leaching fraction and oxide-binding-HA were the main fractions to affect the sorption rate constant, and for the sorption capacity of phenanthrene, humin was the major fraction, followed by oxide-binding-HA, oxide-binding-FA, and so on. While for PCP, the factors of influence on sorption capacity were mainly CEC, Ca-binding-FA, and Ca-binding-HA. [ABSTRACT FROM AUTHOR]
- Published
- 2005
15. Effect of substrate concentration on the bioleaching of heavy metals from sewage sludge.
- Author
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Chen Ying-Xu, Hua Yu-Mei, and Zhang Shao-Hui
- Subjects
HEAVY metals ,BACTERIAL leaching ,POTASSIUM ,ALKALI metals ,NITROGEN ,WASTE management - Abstract
The effect of elemental sulfur concentration on bioleaching of Cu, Zn and Pb and loss of fertilizer value from sewage sludge was investigated in flasks by batch experiments. The results showed that the ultimate pH of sludges with 3-5 g/L of sulfur added was about 1.3 and the production of SO
4 2+ had good correlation with the elemental sulfur concentration. The sensitivity of removal efficiency of metals to sulfur concentration was: Pb > Cu > Zn. The sulfur concentration except for 3 - 5 g/L had significant effect on the solubilization of Cu, Pb and Zn. The highest solubilization efficiency for sludge with 3 g/L of sulfur was 87.86% for Cu, 32.72% for Pb and 92.14% for Zn which could make the treated sludge easily meet the metal limitations for land application. The sulfur concentration of 3 g/L was enough for the solubilization of all three heavy metals. The influence of sulfur concentration on solubilization of total nitrogen and potassium from sludge was negligible. but that on solublization of total phosphorus was of great importance. The loss of nitrogen phosphorus and potassium of sludge with 3 g/L of sulfur by bioleaching was 38.2%, 52.1% and 42.8% respectively, and the sludge still remained satisfactory fenilizer value after bioteaching. [ABSTRACT FROM AUTHOR]- Published
- 2004
16. Reduction of nitrate from groundwater: powder catalysts and catalytic membrane.
- Author
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Chen Ying-xu, S. F., Zhang Yan, and Liu Hong-yuan, S. F.
- Subjects
CATALYSTS ,HYDROGENATION ,BIOLOGICAL membranes ,NITRATES ,AMMONIUM ,GROUNDWATER - Abstract
The reduction of nitrate contaminant in groundwater has gained renewed and intensive attention due to the environmental problems and health risks. Catalytic denetrification presents one one of the most promising approaches for the removal of nitrate from water. Catalytic nitrate reduction from water by powder catalysts and catalytic membrane in a batch reactor was studied. And the effects of the initial concentration, the amounts of catalyst, and the flux H[sub2] on the nitrate reductiion were also discussed. The results demonstrated that nitrate reduction activity and the selectivity to nitrogen gas were mainly controlled by diffusion limitations and the mass transfer of the reactants. The selectivity can improved while retaining a high catalytic activity under conrolled diffusion condition or the intensification of the mass transfer, and a good reaction condition. The total nitrogen removal efficiency reached above 80%. Moreover, catalytic membrane can create a high effective gas/liquid/solid interface, and show a good selectivity to nitrogen in comparative with the power catalyst, the selectivity to nitrogen was improved from 73.4% to 89.4%. [ABSTRACT FROM AUTHOR]
- Published
- 2003
- Full Text
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