11 results on '"Fengwen Xu"'
Search Results
2. Immunogenicity of a vaccinia virus-based severe acute respiratory syndrome coronavirus 2 vaccine candidate.
- Author
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Shan Mei, Zhangling Fan, Xiaoman Liu, Fei Zhao, Yu Huang, Liang Wei, Yamei Hu, Yu Xie, Liming Wang, Bin Ai, Chen Liang, Fengwen Xu, and Fei Guo
- Subjects
SARS-CoV-2 ,COVID-19 vaccines ,GENETIC vectors ,VACCINIA ,IMMUNE response - Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines provide essential tools for the control of the COVID-19 pandemic. A number of technologies have been employed to develop SARS-CoV-2 vaccines, including the inactivated SARS-CoV-2 particles, mRNA to express viral spike protein, recombinant spike proteins, and viral vectors. Here, we report the use of the vaccinia virus Tiantan strain as a vector to express the SARS-CoV-2 spike protein. When it was used to inoculate mice, robust SARS-CoV-2 spike protein-specific antibody response and T-cell response were detected. Sera from the vaccinated mice showed strong neutralizing activity against the ancestral Wuhan SARS-CoV-2, the variants of concern (VOCs) B.1.351, B.1.617.2, and the emerging B.1.1.529 (omicron). This finding supports the possibility of developing a new type of SARS-CoV-2 vaccine using the vaccinia virus vector. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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3. Chemokine (C-X-C Motif) Ligand 4 Is a Restrictor of Respiratory Syncytial Virus Infection and an Indicator of Clinical Severity.
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Zibo Han, Jian Rao, Zhengde Xie, Conghui Wang, Baoping Xu, Suyun Qian, Yingying Wang, Junlin Zhu, Bin Yang, Fengwen Xu, Xiaobo Lei, Fei Guo, Zhendong Zhao, Lili Ren, Jianwei Wang, Han, Zibo, Rao, Jian, Xie, Zhengde, Wang, Conghui, and Xu, Baoping
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CHEMOKINES ,RESPIRATORY syncytial virus ,BIOMARKERS ,LIGANDS (Biochemistry) ,VIRAL load ,DNA analysis ,CYTOKINES ,RESEARCH ,RESEARCH methodology ,ANTIVIRAL agents ,EVALUATION research ,MEDICAL cooperation ,SEVERITY of illness index ,COMPARATIVE studies ,RESPIRATORY syncytial virus infections - Abstract
Rationale: Respiratory syncytial virus (RSV) is the leading cause of childhood respiratory infections worldwide; however, no vaccine is available, and treatment options are limited. Identification of host factors pivotal to viral replication may inform the development of novel therapies, prophylaxes, or diagnoses.Objectives: To identify host factors involved in RSV replication and to evaluate their potential for disease management.Methods: A gain-of-function screening was performed on the basis of a genome-wide human complementary DNA library screen for host factors involved in RSV replication. The antiviral mechanism of CXCL4 (chemokine [C-X-C motif] ligand 4) was analyzed. Its clinical role was evaluated via nasopharyngeal aspirates and plasma samples from patients with RSV infection and different disease severities.Measurements and Main Results: Forty-nine host factors restricting RSV replication were identified by gain-of-function screening, with CXCL4 showing the strongest antiviral effect, which was secretion dependent. CXCL4 blocked viral attachment through binding to the RSV main receptor heparan sulfate, instead of through interacting with RSV surface proteins. Intranasal pretreatment with CXCL4 alleviated inflammation in RSV-infected mice, as shown by decreased concentrations of tumor necrosis factor and viral load in BAL fluid samples as well as by viral nucleocapsid protein histological staining in lungs. Compared with non-RSV infections, RSV infections induced elevated CXCL4 concentrations both in plasma and airway samples from mice and pediatric patients. The airway CXCL4 concentration was correlated with viral load and disease severity in patients (P < 0.001).Conclusions: Our results suggest that CXCL4 is an RSV restriction factor that can block viral entry and serve as an indicator of clinical severity in RSV infections. [ABSTRACT FROM AUTHOR]
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- 2020
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4. MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein.
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Jian Li, Siqi Hu, Fengwen Xu, Shan Mei, Xiaoman Liu, Lijuan Yin, Fei Zhao, Xiaoxiao Zhao, Hong Sun, Zichen Xiong, Di Zhang, Shan Cen, Jianwei Wang, Chen Liang, and Fei Guo
- Subjects
INFLUENZA A virus ,HIV ,CYTOPLASM ,HEPATITIS C virus ,NUCLEOPROTEINS - Abstract
MOV10 has emerged as an important host antiviral factor. MOV10 not only inhibits various viruses, including human immunodeficiency virus type 1, hepatitis C virus and vesicular stomatitis virus, but also restricts the activity of retroelements long interspersed nucleotide element-1, Alu, SVA and intracisternal A particles. Here, we report that MOV10 suppresses influenza A virus infection through interacting with viral nucleoprotein (NP), sequestering viral RNP in the cytoplasm and causing the degradation of viral vRNA. The antiviral activity of MOV10 depends on the integrity of P-bodies. We also found that the antiviral activity of MOV10 is partially countered by viral NS1 protein that interferes with the interaction of MOV10 with viral NP and causes MOV10 degradation through the lysosomal pathway. Moreover, NS1-defective influenza A virus is more susceptible to MOV10 restriction. Our data not only expand the antiviral spectrum of MOV10 but also reveal the NS1 protein as the first viral antagonist of MOV10. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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5. BST-2 restricts IAV release and is countered by the viral M2 protein.
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Siqi Hu, Lijuan Yin, Shan Mei, Jian Li, Fengwen Xu, Hong Sun, Xiaoman Liu, Shan Cen, Chen Liang, Ailing Li, and Fei Guo
- Subjects
VIRAL proteins ,INFLUENZA A virus ,VIRUS-like particles ,BONE marrow cells ,DOWNREGULATION ,HIV - Abstract
BST-2 (tetherin, CD317, and HM1.24) is induced by interferon and restricts virus release by tethering the enveloped viruses to the cell surface. The effect of BST-2 on influenza A virus (IAV) infection has been inconclusive. In the present study, we report that BST-2 diminishes the production of IAV virus-like particles (VLPs) that are generated by viral neuraminidase and hemagglutinin proteins to a much greater degree than it inhibits the production of wild-type IAV particles. This relatively weaker inhibition of IAV is associated with reduction in BST-2 levels, which is caused by the M2 protein that interacts with BST-2 and leads to down-regulation of cell surface BST-2 via the proteasomal pathway. Similarly to the viral antagonist Vpu, M2 also rescues the production of human immunodeficiency virus-1 VLPs and IAV VLPs in the presence of BST-2. Replication of wild-type and the M2-deleted viruses were both inhibited by BST-2, with the M2-deleted IAV being more restricted. These data reveal one mechanism that IAV employs to counter restriction by BST-2. [ABSTRACT FROM AUTHOR]
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- 2017
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6. BoHV-4 immediate early 1 gene is a dispensable gene and its product is not a bone marrow stromal cell antigen 2 counteracting factor.
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Franceschi, Valentina, Capocefalo, Antonio, Jacca, Sarah, Rosamilia, Alfonso, Cavirani, Sandro, Fengwen Xu, Wentao Qiao, and Donofrio, Gaetano
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HERPESVIRUS diseases in animals ,MESENCHYMAL stem cells ,ANTIGENS ,ARTIFICIAL chromosomes ,GENE knockout - Abstract
Background: Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus whose genome was cloned as Bacterial Artificial Chromosome (BAC) and exploited as a gene delivery vector for vaccine purposes. Although BoHV-4 genome has been completely sequenced and its open reading frames (ORFs) structurally defined in silico, most of them are not functionally characterized. In BoHV-4 genome two major immediate early genes (IE) are present, IE1 and IE2. IE2 is an essential gene because its removal from the viral genome renders the virus unable to replicate, whereas for IE1 no many functional information are available. Results: In this work, IE1 contribution in initiating and maintaining BoHV-4 lytic replication was assessed generating a recombinant BoHV-4 genome lacking of IE1 gene, BoHV-4?IE1. In contrast to BoHV-4IE2 deleted mutant, BoHV-4?IE1 infectious replicating viral particles (IRVPs) could be reconstituted following viral DNA electroporation in permissive cells. However the titer of BoHV-4?IE1 IRVPs produced into the cell supernatant and BoHV-4?IE1 plaques size were reduced respect to BoHV-4 undeleted control. Further the impaired BoHV-4?IE1 IRVPs produced into the cell supernatant could be rescued by expressing IE1 gene product in trans, confirming the implication of IE1 in BoHV-4 lytic replication. Next, the possible role of BoHV-4IE1 as bone marrow stromal cell antigen 2 (BST-2) counteracting factor, as hypothesized by IE1 amino-terminal gene product homology with Kaposi Sarcoma Associated Herpesvirus (KSHV) K5, was excluded too. Conclusions: Although the real function of BoHV-4IE1 is still elusive, a new BoHV-4 genome gene locus as a target site for the insertion of foreign DNA and resulting in the attenuation of the virus has been revealed. These data can be considered of relevance to improve BoHV-4 gene delivery properties. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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7. BoHV-4 immediate early 1 gene is a dispensable gene and its product is not a bone marrow stromal cell antigen 2 counteracting factor.
- Author
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Franceschi, Valentina, Capocefalo, Antonio, Jacca, Sarah, Rosamilia, Alfonso, Cavirani, Sandro, Fengwen Xu, Wentao Qiao, and Donofrio, Gaetano
- Abstract
Background: Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus whose genome was cloned as Bacterial Artificial Chromosome (BAC) and exploited as a gene delivery vector for vaccine purposes. Although BoHV-4 genome has been completely sequenced and its open reading frames (ORFs) structurally defined in silico, most of them are not functionally characterized. In BoHV-4 genome two major immediate early genes (IE) are present, IE1 and IE2. IE2 is an essential gene because its removal from the viral genome renders the virus unable to replicate, whereas for IE1 no many functional information are available. Results: In this work, IE1 contribution in initiating and maintaining BoHV-4 lytic replication was assessed generating a recombinant BoHV-4 genome lacking of IE1 gene, BoHV-4ΔIE1. In contrast to BoHV-4IE2 deleted mutant, BoHV-4ΔIE1 infectious replicating viral particles (IRVPs) could be reconstituted following viral DNA electroporation in permissive cells. However the titer of BoHV-4ΔIE1 IRVPs produced into the cell supernatant and BoHV-4ΔIE1 plaques size were reduced respect to BoHV-4 undeleted control. Further the impaired BoHV-4ΔIE1 IRVPs produced into the cell supernatant could be rescued by expressing IE1 gene product in trans, confirming the implication of IE1 in BoHV-4 lytic replication. Next, the possible role of BoHV-4IE1 as bone marrow stromal cell antigen 2 (BST-2) counteracting factor, as hypothesized by IE1 amino-terminal gene product homology with Kaposi Sarcoma Associated Herpesvirus (KSHV) K5, was excluded too. Conclusions: Although the real function of BoHV-4IE1 is still elusive, a new BoHV-4 genome gene locus as a target site for the insertion of foreign DNA and resulting in the attenuation of the virus has been revealed. These data can be considered of relevance to improve BoHV-4 gene delivery properties. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
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8. Characterization of the interactions between SIVrcm Vpx and red-capped mangabey SAMHD1.
- Author
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Jian Li, Fengwen Xu, Siqi Hu, Jinming Zhou, Shan Mei, Xiaoxiao Zhao, Shan Cen, Qi Jin, Chen Liang, and Fei Guo
- Subjects
HIV prevention ,PHOSPHATASES ,VIRAL proteins ,UBIQUITIN ligases ,CD4 antigen ,LENTIVIRUSES ,DNA-binding proteins ,PROTEASOMES - Abstract
SAMHD1 (SAM domain- and HD domain-containing protein 1) inhibits HIV-1 infection of myeloid cells and resting CD4+ Tcells. Two lineages of primate lentiviruses, the sooty mangabey SIV (simian immunodeficiency virus) (SIVsm)/macaque SIV (SIVmac)/HIV-2 lineage and the red-capped mangabey SIV (SIVrcm) lineage, carry a SAMHD1 antagonist called Vpx. Vpx recognizes SAMHD1 and recruits a ubiquitin E3 ligase complex that is composed of CUL4 (Cullin4), DDB1 (damaged DNA-binding protein 1) and a member of the DCAF (DDB1/CUL4-associated factor) family called DCAF1. This E3 ligase complex polyubiquitinates SAMHD1, which leads to proteasomal degradation of SAMHD1. As opposed to the wellcharacterized interaction of SIVmac Vpx with human SAMHD1 and DCAF1, SIVrcm Vpx adopts a different mode of interaction with SAMHD1 of red-capped mangabeys. In the present study, we have characterized the interactions that are essential for SIVrcm Vpx-mediated degradation of rcmSAMHD1 (redcapped mangabey SAMHD1). Using mutagenesis and molecular modelling, we have determined the key role of the W
23 LHR26 peptide of SIVrcm Vpx in recognizing rcmSAMHD1. The amino acids Phe15 , Leu36 , Phe52 , Arg55 and Arg56 at the N-terminal domain (NtD) of rcmSAMHD1 are involved in interaction with Vpxrcm (red-capped mangabey Vpx). The molecular model of rcmSAMHD1-NtD, Vpxrcm and C-terminal domain (CtD) of DCAF1 (DCAF1-CtD) complex reveals further that rcmSAMHD1-NtD and Vpxrcm utilize an interaction interface that is different from that used by human SAMHD1-CtD and Vpxsm. These findings provide further insights into the different modes of interaction between Vpx and SAMHD1 as the result of the 'arms race' of virus and host cell. [ABSTRACT FROM AUTHOR]- Published
- 2015
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9. Identification of novel key amino acids at the interface of the transmembrane domains of human BST-2 and HIV-1 Vpu.
- Author
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Xiaojing Pang, Siqi Hu, Jian Li, Fengwen Xu, Shan Mei, Jinming Zhou, Shan Cen, Qi Jin, and Fei Guo
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MESENCHYMAL stem cells ,AMINO acids ,CELLULAR signal transduction ,HIV ,FLUORESCENCE resonance energy transfer ,MOLECULAR dynamics - Abstract
Background: BST-2 (bone marrow stromal cell antigen 2) is an interferon-inducible protein that inhibits virus release by tethering viral particles to the cell surface. This antiviral activity of BST-2 is antagonized by HIV-1 accessory protein Vpu. Vpu physically interacts with BST-2 through their mutual transmembrane (TM) domains. In this study, we utilized the BRET assay and molecular dynamics (MD) simulation method to further characterize the interaction of BST-2 and Vpu. Results: Amino acids I34, L37, P40 and L41 in the TM domain of BST-2, and L11, A18 and W22 in the TM domain of Vpu were identified to be critical for the interaction between BST-2 and Vpu. The residues P40 in the TM domain of BST-2 and L11 in the TM domain of Vpu were shown, for the first time, to be important for their interaction. Furthermore, triple-amino-acid substitutions, 14–16 (AII to VAA) and 26–28 (IIE to AAA) in Vpu TM, not the single-residue mutation, profoundly disrupted BST-2/Vpu interaction. The results of MD simulation revealed significant conformational changes of the BST-2/Vpu complex as a result of mutating P40 of BST-2 and L11, 14–16 (AII to VAA) and 26–28 (IIE to AAA) of Vpu. In addition, disrupting the interaction between BST-2 and Vpu rendered BST-2 resistant to Vpu antagonization. Conclusions: Through use of the BRET assay, we identified novel key residues P40 in the TM domain of BST-2 and L11 in the TM domain of Vpu that are important for their interaction. These results add new insights into the molecular mechanism behind BST-2 antagonization by HIV-1 Vpu. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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10. Tetherin inhibits prototypic foamy virus release.
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Fengwen Xu, Juan Tan, Ruikang Liu, Dan Xu, Yue Li, Yunqi Geng, Chen Liang, and Wentao Qiao
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INTERFERONS ,RETROVIRUSES ,HERPESVIRUSES ,GLYCOSYLATION ,HIV - Abstract
Background: Tetherin (also known as BST-2, CD317, and HM1.24) is an interferon- induced protein that blocks the release of a variety of enveloped viruses, such as retroviruses, filoviruses and herpesviruses. However, the relationship between tetherin and foamy viruses has not been clearly demonstrated. Results: In this study, we found that tetherin of human, simian, bovine or canine origin inhibits the production of infectious prototypic foamy virus (PFV). The inhibition of PFV by human tetherin is counteracted by human immunodeficiency virus type 1 (HIV-1) Vpu. Furthermore, we generated human tetherin transmembrane domain deletion mutant (delTM), glycosyl phosphatidylinositol (GPI) anchor deletion mutant (delGPI), and dimerization and glycosylation deficient mutants. Compared with wild type tetherin, the delTM and delGPI mutants only moderately inhibited PFV production. In contrast, the dimerization and glycosylation deficient mutants inhibit PFV production as efficiently as the wild type tetherin. Conclusions: These results demonstrate that tetherin inhibits the release and infectivity of PFV, and this inhibition is antagonized by HIV-1 Vpu. Both the transmembrane domain and the GPI anchor of tetherin are important for the inhibition of PFV, whereas the dimerization and the glycosylation of tetherin are dispensable. [ABSTRACT FROM AUTHOR]
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- 2011
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11. The interferon-induced MxB protein inhibits an early step of HIV-1 infection.
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Zhenlong Liu, Qinghua Pan, Shilei Ding, Jin Qian, Fengwen Xu, Jinming Zhou, Shan Cen, Fei Guo, and Liang, Chen
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HIV infections ,INTERFERONS - Abstract
An abstract of the article "The interferon-induced MxB protein inhibits an early step of HIV-1 infection" by Zhenlong Liu, Qinhua Pan, Shilei Ding, Jin Qian, Fengwen Xu, Jinming Zhou, Shan Cen, Fei Guo and Chen Liang is presented.
- Published
- 2013
- Full Text
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