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Summary In contrast to supplemented microbial phytases, considerably lower cereal phytase activities were found after application of an enzyme solution extracted in citrate/NaOH buffer (pH 5.5) for 1 h as compared with the direct incubation of the plant material. Differences between both methods obtained were 70% for wheat and spelt and 50% for barley and rye. The determination of phytase activity of a wheat sample by direct incubation was affected by the sample size and amount of substrate (Na-phytate) added. Optimal conditions are a maximal level of phytase activity of ≤ 0.05 U incubated for 60 min in a buffered solution (citrate/NaOH, pH 5.5) with 10 ml of 32.6 mmol/l Na-phytate at 37 ∘ C. Possible reasons for the differences between both methods and for the factors affecting phytase activity by direct incubation are discussed. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Animal Physiology & Animal Nutrition is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Animal Physiology & Animal Nutrition is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Animal Physiology & Animal Nutrition is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The objective of this study was to investigate the influence of microbial phytase and calcium supplementation to diets for growing pigs on the retention of lead in the kidney, liver, muscle, brain, and bone (phalanx 1). The experiments were carried out with barrows over the body weight range from 17 to 50 kg. The average lead concentration of the diets was 1.45 mg/kg dry matter. Diets were prepared with or without a supplement of 800 units of microbial phytase. The calcium concentration in the diets was 6.53 or 13.4 g/kg dry matter. The addition of microbial phytase showed an increase of lead concentration in bone. By increasing the calcium concentration to 13.4 g/kg dry matter, it was possible to avoid the phytase-induced increase of lead retention in bone. [ABSTRACT FROM AUTHOR]