1. Characterization of a novel multi-resistant Pseudomonas juntendi strain from China with chromosomal blaVIM−2 and a megaplasmid coharboring blaIMP−1−like and blaOXA−1.
- Author
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Jiang, Shiman, Li, Yaling, Bi, Kefan, Yang, Sisi, Xia, He, Li, Shengjie, Chen, Hui, and Li, Lanjuan
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WHOLE genome sequencing ,MICROBIAL sensitivity tests ,GENETIC variation ,GENETIC mutation ,PSEUDOMONAS - Abstract
Background: Pseudomonas juntendi is a newly identified opportunistic pathogen, of which we have limited understanding. P. juntendi strains are often multidrug resistant, which complicates clinical management of infection. Methods: A strain of Pseudomonas juntendi (strain L4326) isolated from feces was characterized by MALDI-TOF-MS and Average Nucleotide Identity BLAST. This strain was further subject to whole-genome sequencing and Maximum Likelihood phylogenetic analysis. The strain was phenotypically characterized by antimicrobial susceptibility testing and conjugation assays. Results: We have isolated the novel P. juntendi strain L4236, which was multidrug resistant, but retained sensitivity to amikacin. L4236 harbored a megaplasmid that encoded bla
OXA−1 and a novel blaIMP−1 resistance gene variant. P. juntendi strain L4236 was phylogenetically related to P. juntendi strain SAMN30525517. Conclusion: A rare P. juntendi strain was isolated from human feces in southern China with a megaplasmid coharboring blaIMP−1−like and blaOXA−1 . Antimicrobial selection pressures may have driven acquisition of drug-resistance gene mutations and carriage of the megaplasmid. [ABSTRACT FROM AUTHOR]- Published
- 2024
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