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Nigeria ranks second in the global prevalence of diarrhoea with most cases concentrated in the Northern-region of the country. This research explored the antidiarrhoeal efficacy of the hydroethanolic extract of Piliostigma thonningii leaves (HEPTL), locally used to manage diarrhoeal conditions in Kebbi State, Nigeria. P. thonningii leaves were screened for their secondary metabolites and mineral constituents. Using 3 standard-diarrhoea models, female Wistar rats completely-randomised into six-groups of six animals each were utilised for probing the antidiarrhoeal activity of HEPTL. Animals in groups I and II served as the negative and positive controls, whereas the rats in groups III, IV and V respectively received 50, 100, and 200 mg/kg body weight-(bw) of HEPTL. Six secondary metabolites and eight minerals were found in the extract, with flavonoids and calcium being the most abundant while steroids and zinc were the least prevalent, respectively. High performance liquid chromatographic analysis revealed the presence of 19 bioactive substances. Furthermore, there was a significant (p<0.05) and dose-related reduction in diarrhoea onset, water content, and wet faeces count. Similarly, the amount of intestinal fluid and average distance traversed by the charcoal-meal were decreased dose-dependently by the HEPTL with a commensurate rise in the suppression of intestinal fluid accrual and peristalsis. Acetylcholinesterase, Na+/K+-ATPase, reduced glutathione, intestinal-alkaline phosphatase and protein levels increased significantly (p<0.05) whereas superoxide-dismutase, catalase, intestinal-nitric oxide and malondialdehyde levels all fell significantly (p<0.05). However, the level of intestinal glucose was not significantly altered. Overall, the HEPTL exhibited a profound effect in the alleviation of the severity of diarrhoea, notably at 200 mg/kg bw. [ABSTRACT FROM AUTHOR]

Tramadol, a widely used analgesic, is becoming more abused in most countries. Hence, this study aims to investigate the protective effects of aqueous extract of Piper nigrum whole fruits on tramadol-induced toxicity in male Wistar rats. Six groups (A-F) of male Wistar rats were exposed to a 14-day oral treatment. Specifically, the control group, induced group (tramadol at 60 mg/kg), reference drug group (Vitamin C at 250 mg/kg) and the extract groups (250, 500, and 1000 mg/kg). The levels of liver cellular markers ALT, AST and ALP as well as the kidney function parameters (urea, creatinine and uric acid) showed a significant (p<0.05) increase following the administration of 60 mg/kg of tramadol and a significant reversal was observed following the administration of extract in a dose dependent manner. However, K+ and HCO3-showed a significant (p< 0.05) decrease and were also reversed following the administration of extract in a dose dependent manner. Tramadol also showed a significant (p<0.05) increase in the concentration of WBC and LYM, and a significant (p< 0.05) decrease in the levels of RBC, HGB, HCT, RDW-SD, RDW-CV, and MPV. The reference drug and the extract showed significant (p< 0.05) reversal in the levels of these biochemical parameters. Histological examination revealed normal liver and kidney architecture in all groups, indicating no signs of inflammation or damage. These findings revealed that the aqueous extracts from whole fruits of P. nigrum at concentrations of 250 and 500 and 1000 mg/kg may potentially offer protection and general improvement in the overall health status and function of the hematopoietic system. [ABSTRACT FROM AUTHOR]