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The UN Decade on Ecosystem Restoration will result in an unprecedented need for seeds. Agricultural production, or the growing of plants under controlled conditions to produce desired resources, can be a helpful tool for providing the quantities of seeds needed for large‐scale restoration. In some ecosystems, agricultural production of native plant seeds is part of the restoration process. This is particularly true for native plants that are similar to major food crops, such as native grasses. However, conventional crops have contrasting characteristics (e.g. fast growing, high yield for a focused use) from those desired in restored vegetation (plants capable of surviving and providing ecosystem services in variable environments). This contrast leads to significant, often opposing differences in desirable characteristics in crop versus natural systems. Examples include rapid germination in domesticated crops versus seed dormancy in wild plants, uniform germination, growth and flowering versus bet‐hedging strategies, or high seed retention versus seed dispersal. While it may be useful to maximize productivity of native plants in agricultural environments with similar trait modifications, for restoration, the demands of the natural environment should be primary, and agricultural production should aim to maximize the diversity, function, and evolutionary potential of native species. Any perceived benefits that come from the production of large quantities of inexpensive seeds that cannot meet the needs of wild communities are short‐sighted. We suggest research directions that will be needed to meld agricultural production needs with those of restoration practitioners, as a challenge for those invested in the procurement and use of wild seeds. [ABSTRACT FROM AUTHOR]

Zani WNWM, Sidik NJ, Isa NAM, Awal A, Osman NI, Alias N, Nordin MK. 2024. Influence of media variations and growth regulators on in vitro propagation of Dendrocalamus asper bamboo. Nusantara Bioscience 16: 237-244. This study reports the effects of different types of media and combinations of plant growth regulators on the in vitro propagation of Dendrocalamus asper (Schult.f.) Backer bamboo species, a species known for its economic significance and challenges in traditional cultivation. The experiment was initiated through the cultivation of in vitro nodal segments as explants in various strengths (half-strength and full-strength) of Murashige and Skoog's (MS) and Vacin & Went (VW) media, supplemented with 1.0 mg L-1 6-benzylaminopurine (BAP) hormone. Optimal results were achieved in full-strength MS media with 1.0 mg L-1 BAP, with the highest shoot number (5.6 shoots) and length (2.14±0.18 cm), surpassing outcomes in VW media. Shoot multiplication in full-strength MS media, with varying BAP and indole-3-butyric acid (IBA) hormone combinations (0.5, 1.0, 2.0, and 4.0 mg L-1), was conducted. The combination of 4.0 mg L-1 BAP and 0.5 mg L-1 IBA yielded the highest number of shoots (5.17±3.97), while supplementation with 4.0 mg L-1 IBA alone resulted in the longest shoot with 1.89±1.15 cm. These findings underscore the significance of tailored conditions for optimal in vitro propagation of this species. Further investigations could explore additional factors influencing the propagation process for better refinement of bamboo cultivation techniques. [ABSTRACT FROM AUTHOR]

Full-spectrum light-emitting diodes (LEDs) have gradually replaced narrowspectrum LEDs and are widely used in plant factories with artificial lighting (PFALs). However, the specific effect of LED light quality on dry mass allocation in runner plant propagation remains unclear. Hence, we cultivated "Akihime" strawberries as mother plants for 115 days to conduct runner plant propagation experiment under white LEDs (W100), white and red LEDs (W84R16 and W55R45), red and blue LEDs (RB100), and red, blue and green LEDs (RB80G20) in PFALs, and determined key factors affecting dry mass accumulation and allocation among mother plants and runner plants based on growth component analysis. The results showed that the net photosynthetic rate and total leaf area in mother plants in W100 increased by 11% and 31%, respectively, compared withW55R45. In comparison toW84R16 andW55R45, W100 increased the dry mass (23%-30%) of runner plants mainly by increasing the total dry mass (TDM) (23%) of strawberry plants, without significantly affecting the fraction of drymass partitioning to runner plants. However, the number of runners in W55R45 was 5.1 per plant, representing only 78% of that in W100. Compared with RB100, RB80G20 significantly increased the number of runner plants and runner numbers by 16% and 19% to 13.0 per plant and 5.8 per plant, respectively. The partial replacement of blue light with green light in RB80G20 induced a shade avoidance response in runner plants, resulting in a 55% increase in the total leaf area of runner plants compared with RB100. Data from growth component analysis showed that compared with red and blue LEDs, white LEDs increased the TDM of runner plants by 83% by increasing the plant TDM accumulation (44%) and the fraction of dry mass partitioning to runner plants (37%). Additionally, the dry mass (g) of runner plants per mol and per kilowatt-hour under in W100 were 0.11 and 0.75, respectively, significantly higher than other treatments. Therefore, reducing red light proportion in full-spectrum LEDs is beneficial for strawberry runner plant propagation in PFALs. [ABSTRACT FROM AUTHOR]

Chenopodium hircinum, the putative wild ancestor of quinoa, is a source of traits that could improve the tolerance of crop quinoa to high temperatures. However, seeds of C. hircinum have physiological dormancy (PD), which is an obstacle for plant propagation and use in breeding programs. We studied the intraspecific variability in morpho-anatomical traits of embryo covering structures and their association with PD. We also evaluated the effects of different dormancy-breaking treatments on PD alleviation and germination. Seeds were dispersed with a remnant perianth and a persistent pericarp that could be removed by scraping. The seed coat was formed by palisade cells impregnated with tannins, and the seed contained a thin layer of peripheral endosperm surrounding the embryo. In our investigation, the thickness of the pericarp (P) and/or seed coat (SC) varied among populations. Populations with higher P and/or SC thickness showed lower percentages of germination and water absorption. The combined dormancy-breaking treatment (bleach + perforated coverings + gibberellic acid) promoted dormancy release and increased germination. C. hircinum seeds showed non-deep physiological dormancy. Based on previous knowledge about quinoa, and our results, we conclude that embryo coverings, especially the seed coat, have an important role in dormancy control, imposing a mechanical restraint on radicle emergence. [ABSTRACT FROM AUTHOR]

This study investigates the effect of different cutting types and planting media on the growth of Indigofera zollingeriana, a valuable forage crop. The research used a factorial randomized block design (2 x 2) with 40 experimental units. The treatments included two cutting sources--stem cuttings (A1) and shoot cuttings (A2)--and two planting media--natural soil (B1) and natural soil supplemented with liquid organic fertilizer from rabbit urine (B2). Growth parameters such as the number of leaves, plant height, and stem diameter were measured. The results indicate that cutting type significantly affects leaf production, with shoot cuttings (A2) producing a higher number of leaves than stem cuttings (A1) (P<0.05). However, the planting media and its interaction with the cutting type had no significant impact on plant height and stem diameter (P>0.05). These findings suggest that using shoot cuttings could enhance the propagation and growth of Indigofera zollingeriana for sustainable forage production. Further studies are recommended to explore the long-term effects of organic fertilizer on biomass yield. [ABSTRACT FROM AUTHOR]

This research was carried out under field conditions to determine the effect of PGPB on the yield and quality of Pulsar F1 eggplant variety under Igdir ecological conditions in 2021. The experiment was conducted with 8 treatments (Brevibacillus reuszeri strain IT 119, Kluyvera cyrocrescens strain IT 160, IT 119 + IT 160, IT 119 + Fertilizer, IT 160 + Fertilizer, IT 119 + IT 160 + Fertilizer, Fertilizer and Negative Control) with 3 replications according to the randomized block design. Bacterial strains were applied by inoculating 100 ml (106 cfu/ml) to the root zone of the plants during seedling stage. PGPB treatments increased total yield, discard yield, marketable yield, plant height, plant stem thickness, number of leaves, plant root wet weight, plant root dry weight, stem wet weight, stem dry weight, number of fruits per plant, fruit wet weight, fruit dry weight and root length compared to negative and positive control groups. The treatments had no effect on fruit diameter. Although both strains were found to be successful, especially Kluyvera cyrocrescens strain IT 160 alone and in combination showed positive effects in terms of the parameters examined in eggplant plants and has the potential to be used as biofertilizer. [ABSTRACT FROM AUTHOR]

This study was conducted in Çanakkale province (North-West Anatolia) to determine the optimal auxin dosages (IBA at 2000 ppm and NAA at 1000 ppm) and rooting media (soil, peat, and cocopeat) for the cutting propagation of lavender, which holds significant industrial and commercial value. The research utilized three varieties belonging to the Lavandula angustifolia Mill species: Hemus, Sevtopolis and Drujba, as well as the Super A variety from the Lavandula × intermedia Emeric ex Loisel species. The experiment was organized with three replications, following a split-plot trial design divided into random plots. Results indicated that the Super A variety of Lavandula × intermedia Emeric ex Loisel, when cultivated in cocopeat medium with IBA at 2000 ppm, achieved the highest rooting rate (88.33%) and rooting status (17.67 units). The Sevtopolis variety of the Lavandula angustifolia species exhibited the highest values for root number (15.33), root length (9.88 cm), shoot number (20.93), and shoot length (15.35 cm) when grown in cocopeat medium with IBA at 2000 ppm. Based on these findings, the use of cocopeat medium and IBA at 2000 ppm auxin application is recommended for the effective propagation of lavender by cuttings. [ABSTRACT FROM AUTHOR]

The aim of this study was to determine the propagation potential of some local varieties using the in vitro shoot-tip culture method. Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı were used as materials. As a result of the study, the values of rooting rate, number of roots, length of roots, number of shoots, length of shoots, number of leaves and number of nodes of the grape varieties were determined. When the cultivars were compared in terms of rooting characteristics, the highest rooting rate was 54.7%, the highest root number was 3.71 per plant, and the highest average root length value was 17.93 cm from Bilecik İrikarası. When the cultivars were evaluated in terms of shoot length, the highest shoot length value was determined in Razaki variety with 5.72 cm. Similarly, considering the number of leaves and nodes in the shoots were determined, with the highest leaf number value was of 8.71, the highest node number value was of 6.71 in Razakı variety. As a result of the study, local varieties that used as material in this study showed positive results in in vitro propagation. [ABSTRACT FROM AUTHOR]

Begonia rex cv. DS-EYWA is an important plant for indoor and outdoor cultivation, and cv. DS-EYWA is a rare unique cultivar with curly, colorful leaves. Due to their importance, applying plant tissue culture techniques for mass and healthy production in a short period of time without seasonal limitation is of immense economic value. Applying several concentrations of silver nitrate (AgNO3) in combination with varied concentrations of cytokinins including 6-benzylaminopurine (BAP), thidiazuron (TDZ) (0, 0.5, 1, 1.5 mgL−1), and 1-naphthaleneacetic acid (NAA) auxin (0, 0.5, 1 mgL−1) via focusing on transverse thin cell layer (tTCL) petiole explants for high-scale production was used to establish an efficient in vitro propagation protocol. Our results showed that even low concentrations (25 mgL−1) can control internal bacterial infection and increase shoot direct regeneration efficiency. A combination of 1.5 mgL−1 BAP, 0.5 mgL−1 NAA, and 25 mgL−1 AgNO3 was the best treatment to increase the number of direct regenerated shoots, and a lower concentration of BAP (0.5 mgL−1) can be suggested for shoot elongation. Elongated shoots were successfully rooted in MS basal medium and acclimatized in a 1:1 peat moss/perlite sterilized pot mixture. [ABSTRACT FROM AUTHOR]

(1) Background: Willow forests are well established as nature-based solutions contributing to flood protection in the riverine environment. With climate change, storm surges in winter may increasingly expose downstream floodplain forests to seawater pulses. The effects of seawater pulses on willows are unknown, as previous studies focused on long-term exposure effects. (2) Methods: We studied the resilience of the floodplain forest foundation species Salix alba L. to seawater pulses during winter. This corresponds to the effects of storm surges in the North Sea region on floodplain willow trees in downstream river stretches during their dormant stage. Seawater pulses were applied from November to May on vegetative propagules. The plants were placed on flooding stairways at three levels in a mesocosm experiment under ambient conditions in Zealand, NL. (3) Results: Twice-applied 48 h seawater pulses during winter led to increasing salinity in the soil where vegetative propagules were placed. Ninety-five percent of the plants developed leaves, shoots, and roots, and juvenile trees were established in the following spring. Although the aboveground and belowground dry masses decreased with increasing short-term seawater flooding, they increased from April to May. (4) Conclusions: The seawater pulse caused a growth-delaying effect in the young experimental propagation plants of Salix alba. Contrary to earlier findings on the growth-inhibiting effects on S. alba under long-term salinity treatments, we show that S. alba is resilient to short-term seawater pulses experienced during the dormant (winter) stage. This is good news for the inclusion of S. alba in nature-based flood defense schemes in downstream riverine stretches. [ABSTRACT FROM AUTHOR]

Coffee cultivation facilitates foreign trade, which is important to the Mexican economy, particularly to the coffee growers of Jilotepec, Veracruz. However, in this region, the soil in which the coffee plants are grown is acidic and has low nutrient availability, making plants susceptible to pests and diseases. In this context, the use of mycorrhizal fungi has gained importance, due to the benefits that they provide in terms of the transport of nutrients and the development of plants, contributing to a reduction in the use of chemical fertilizers. This work aimed to determine the dominant Arbuscular mycorrhizal fungi (AMF) in the soil of coffee farms and evaluate the potential of sorghum as a trap plant for these organisms. As a result, ten morphotypes of AMF were detected in the coffee soil, with Glomus and Acaulospora being the dominant genera. It was found that their presence was related to the pH, clay, organic matter, and total carbon of the soil from the farms. The abundance of spores increased significantly (p < 0.05) between the initial count in the soil and the final count after propagation in the sorghum trap plants. The characteristic structures of mycorrhizal colonization and a high percentage of mycorrhizal colonization of the roots of the trap plants (Sorghum vulgare) were observed at 120 days after sowing. It is concluded that Glomus sp1, Glomus sp2, Glomus sp3, Glomus sp4, Rhizophagus clarus, and Acaulospora scrobiculata are the dominant morphotypes in the considered coffee plantation soils and that sorghum has high potential for favoring the propagation of native AMF through increasing their abundance and favoring high mycorrhizal colonization. [ABSTRACT FROM AUTHOR]

Copyright of Arab Journal of Plant Protection is the property of Arab Society for Plant Protection and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Carajasia cangae (Rubiaceae) is a narrow endemic species from the canga ecosystems of the Carajás National Forest that is facing extinction due to a limited range and habitat disturbance from hydroclimatological changes and mining activities. This study examines the influence of rhizospheric and endophytic bacteria on C. cangae seed germination to support conservation efforts. Soil samples, both rhizospheric and non-rhizospheric, as well as plant root tissues, were collected. Bacteria from these samples were subsequently isolated, cultured, and identified. DNA sequencing revealed the presence of 16 isolates (9 rhizospheric and 7 endophytic), representing 19 genera and 6 phyla: Proteobacteria, Actinobacteria, Acidobacteria, Firmicutes, Bacteroidetes, and Chloroflexi. The endophytic isolates of Bacillus and the rhizospheric isolates of Planococcus and Lysinibacillus reduced the median germination time and initiation time, while the rhizospheric isolates Serratia and Comamonas increased the germination time and decreased the germination percentage in comparison to the control sample. These findings emphasize the crucial role of endophytic bacteria in the germination of C. cangae and highlight isolates that could have beneficial effects in the following stages of plant growth. Understanding the impact of endophytic and rhizospheric bacterial isolates on seed germination can enhance conservation efforts by shortening the germination period of this species and thereby improving seedling production. Additionally, this knowledge will pave the way for future research on the role of bacteria in the establishment of C. cangae. [ABSTRACT FROM AUTHOR]

Rootstocks/scions with potential for commercial growing, either for the industry or fresh market, have been one of the main objectives of the fruit breeding programs. Fruit crop breeding has impediments like polyembryony (PE) which is otherwise desirable for clonal plant propagation. In nature PE exist in some fruit crops like citrus, mango, jamun, rose apple, almond etc. In polyembryonic crops, there is an occurrence of the more than one embryo in seed and subsequently numerous seedlings emerge from that single seed. The mother type seedlings are considered nucellar are often vigorous in nature as compared to zygotic ones. In this review, the classification, genetic basis and mechanism of PE has been discussed. In current review, morphogenetic authentication of seedling from polyembronic seed has been discussed in the light of recent research. Transcriptional pathway along with the inheritance pattern and molecular breeding aspects of PE has also been summarized. [ABSTRACT FROM AUTHOR]

Paphiopedilum primulinum and Paphiopedilum glaucophyllum have unique labellum colour and shaped like lady's slippers. These orchids are from the Cochlopetalum section, which is exclusively found in Sumatra and Java. There are so many people that desire to collect these plants illegally. Due to extensive commercial exploitation, Paphiopedilum is in danger of going extinct. Tissue culture techniques are utilised to conserve threatened orchid germplasm in a short time. The success of the in vitro culture depends on the accuracy of the basic media composition used. The Ambon Lumut banana (ALB) can accelerate plant growth and cell division. Banana added to the culture medium was prepared by mashing the ripe flesh (3.5 months old) using a mortar. This research aims to investigate the effect of banana homogenate supplemented media for the orchids P. primulinum and P. glaucophyllum based on the parameters of difference of plant height (calculated from the base of the stem to the tip of the plant stem), number of leaves, and number of roots. The measurement method was carried out using a ruler with a centimetre scale. Observations and documentation were carried out once a week for 7 weeks after planting (WAP) for P. primulinum and P. glaucophyllum. The results showed that V Murashige and Skoog (MS) + ALB homogenate is a better medium for P. primulinum and P. glaucophyllum growth than media without banana homogenate. The highest values of plant height, leaf growth and root growth of P. primulinum with banana homogenate were 0.44 cm, 0.63 leaves, and 0.50 roots, respectively. The highest values of plant height and leaf growth of P. glaucophyllum were 0.75 cm and 1.90 leaves, respectively. Culture medium added banana homogenate was able to support the propagation of plants, some of which are returned to nature and others used for industrial purposes (conventionally cultivated by the community). [ABSTRACT FROM AUTHOR]

In this study, the impacts of LEDs on the phytoremediation of arsenic (As) and mercury (Hg) by Bacopa monnieri (L.) Wettst. were investigated, along with the examination of the biochemical characteristics of plants exposed to metal-induced toxicity. In vitro multiple and rapid plant propagations were successfully achieved by adding 1.0 mg/L 6-Benzyl amino purine (BAP) to the Murashige and Skoog (MS) basal salt and vitamin culture medium. For plant-based remediation experiments, different concentrations of As (0–1.0 mg/L) and Hg (0–0.2 mg/L) were added to the water environment, and trials were conducted for four different application periods (1–21 days). White, red, and blue LEDs, as well as white fluorescent light, were preferred as the light environment. The results revealed that LED lights were more effective for heavy metal accumulation, with red LED light significantly enhancing the plant's phytoremediation capacity compared to other LED applications. Moreover, when examining biochemical stress parameters such as levels of photosynthetic pigments, protein concentrations, and lipid peroxidation, plants under red LED light showed better results. Generally, the lowest results were obtained under white fluorescent light. These findings contribute to phytoremediation studies by highlighting the integration of LED lights, thereby enabling the development of a more effective, cost-efficient, and environmentally sustainable remediation system compared to other treatment methods. [ABSTRACT FROM AUTHOR]

Flowering is critical to the success of plant propagation. The MYB family transcription factor CIRCADIAN CLOCK-ASSOCIATED1 (CCA1) is an essential component of the core loop of the circadian clock and plays a crucial role in regulating plant flowering time. In this study, we found that photoperiod affects the expression pattern and expression level of BcCCA1, which is delayed flowering time under short-day conditions in Pak-choi [Brassica campestris (syn. Brassica rapa) ssp. chinensis]. We detected overexpression and silencing of BcCCA1 in Pak-choi, resulting in delayed and promoted flowering time, respectively. Furthermore, we also discovered that FLOWERING LOCUS C (BcFLC) and SUPPRESSOR OF CONSTANS1 (BcSOC1) were expressed significantly differently in BcCCA1 overexpression and silencing plants compared with control plants. Therefore, we further investigated the interaction relationship between BcCCA1, BcFLC, and BcSOC1, and the results showed that BcCCA1 and BcFLC as a complex interacted with each other. Moreover, both BcCCA1 and BcFLC can directly bind to the promoter of BcSOC1 and repress its transcription, and BcCCA1 can form a complex with BcFLC to enhance the transcriptional inhibition of BcSOC1 by BcFLC. This study reveals a new mechanism by which the circadian clock regulates flowering time. [ABSTRACT FROM AUTHOR]

Cassava serves as a crucial raw material and sustenance staple in developing countries, extensively used in the production of starch, bioethanol, and various bioproducts. However, the cultivation of cassava is increasingly challenged by diseases, particularly the cassava mosaic virus. Therefore, efficient propagule systems are needed to provide disease‐free plants to sustain production. This study aimed to develop a rapid propagation method to increase the production of planting material. Various propagation media were assessed for their impact on root formation and plantlet vigour using a 3 × 4 factorial in a Randomized Complete Block Design during April 2023 to September 2023. Three cassava cultivars (KU50, RY9, and HB60) were tested against four media (distilled water, 250 mg·L−1 IBA, SAH solution, and IBA + SAH combination). Subsequently, the optimal medium was used to compare three propagation techniques (leaf bud, mini, and normal cutting) across the cassava cultivars. Aeroponic systems were then employed for further shoot multiplication, evaluating growth and survival metrics. In the first experiment, RY9 had better growth parameters, while KU50 had the highest survival rate at 82%. Applying the SAH was the optimal solution when compared to other media tested. It significantly enhanced the plantlet height (7.30 cm), survival percentage (71.61%), plantlet vigour index (1513.60), and allometric coefficient (0.55). The leaf bud technique had the highest multiplication trait values, whereas the mini‐cutting method had the highest survival rate, and normal cutting had superior performance compared to leaf bud and mini‐cutting for the plant growth parameters. Plantlets produced through mini‐cutting (93%) and leaf bud propagation (92%) had a higher survival rate than those produced using normal cutting (46%) in the aeroponic system under greenhouse conditions. The study revealed a homogeneity in growth and survival traits across the cassava cultivars evaluated in aeroponic cultivation. These findings demonstrated the potential of using leaf bud or mini‐cutting propagation techniques for multiplication in aeroponic cultivation to produce high‐quality plantlets on a large‐scale production. [ABSTRACT FROM AUTHOR]

The present study investigated in vitro multiplication and bulbification in one imported garlic accession i.e. VFG158 and eleven local garlic accessions of Mauritius viz. Beeharry, Boodnah, Bondah, Gooniah, Haulkhory, Ramdhuny, Ramjee, Rampall, Sujeebun, Sujeebun 2 and Unuth through meristem culture. The explants were subjected to fourteen shoot multiplication and three bulbification treatments. Positive results for shoot proliferation and suppression of hyperhydricity were noted on six shoot multiplication media: MS basal media with various growth regulators {G0 (0.25mg/L NAA+0.5mg/L 2iP), G1 (1.5mg/L BAP+0.5mg/L NAA), G2 (0.3mg/L NAA+3mg/L 2iP), G6 (0.5mg/L NAA+2mg/L 2iP), G14(2mg/L BAP+2mg/L NAA) and G15(1mg/L BAP+0.5mg/L NAA)}. The highest number of shoot formation was observed in G2 (0.3mg/L NAA+3mg/L 2iP) and the lowest number of shoot formation was observed in G14 (2mg/L BAP+2mg/L NAA). Genotypic difference in shoot multiplication and hyperhydricity on different media formulation was observed. The highest shoot proliferation was observed in the garlic accession Ramdhuny, while the lowest shoot proliferation was recorded in groups of similar accessions namely, Ramjee, Sujeebun and VFG 158. Bulblet formation was earlier on bulbification medium B2 (MS enriched with 12% sucrose). Largest and heavier bulblets were obtained on medium B5 (MS supplemented with 2mg/L BAP+1mg/L GA3 and enriched with 90% sucrose). A reliable protocol for rapid shoot regeneration and multiplication from meristem-tip culture and bulblet formation from multiple shoot clumps was optimised. [ABSTRACT FROM AUTHOR]

Yam (Dioscorea sp.) is an important staple crop for millions of smallholder farmers in West Africa. In this study, we aimed to develop an efficient indirect plant regeneration system for three important yam genotypes "Kukrupa," "Mankrong Pona" and "Matches." The potential of leaf primordia, petioles and axillary bud explants for callus induction on Murashige and Skoog (MS) medium supplemented with different concentrations (0, 3, 5, 7 and 9 mg/l) of 2,4-dicholorophenoxy acetic acid (2,4-D) or picloram were tested. Callus induction was influenced by auxin concentration as well as explant. The highest callus induction was achieved using leaf primordia ("Kukrupa" and "Mankrong Pona") explants on 3 mg/l picloram or axillary buds ("Matches") on 5 mg/l picloram. Transfer of callus to 6-benzylaminoapurine (BAP) amended medium resulted in the formation of at least two shoots per callus clump. Plantlets were obtained on basic yam medium supplemented with 0.2 mg/l 1-naphthalene acetic acid (NAA), 3 mg/l kinetin and 0.5 mg/l BAP after four weeks. Post acclimatization plantlet survival was high (75 − 82.5%). The indirect plant regeneration protocol reported through this study helps in the improvement of important yam genotypes through tissue culture-oriented genome editing, in vitro mutagenesis and also for its mass propagation. [ABSTRACT FROM AUTHOR]

Brazil is the world's largest producer and consumer of passion fruit; however, productivity is restricted by phytosanitary factors, mainly viruses. Passion fruit woodiness disease (PWD) is the major viral disease of the crop, caused in Brazil by the cowpea aphid-borne mosaic virus (CABMV), and non-persistently transmitted by aphids. In this study, a protocol was developed and validated using real-time RT-PCR with a specific TaqMan probe combined with a direct sample preparation method for the detection of CABMV. The new protocol is 100 times more sensitive than the PTA-ELISA in detecting CABMV in passion fruit. The nucleotide sequence of the CABMV isolates from Santa Catarina (SC), had high identity (above 99%) in the capsid protein (CP) genome region, with isolates from São Paulo (SP). CABMV isolates from SC formed a monophyletic cluster with 99% bootstrap support containing CABMV isolates and strains from SP and Sergipe (SE). The logistic model showed a better fit to the virus incidence data compared to the Gompertz and monomolecular models and was able to predict that half of the orchard would be infected in about 110 days after transplantation (DAT). The TaqMan assay will be used for the detection of CABMV in propagation plant material and insect vectors, contributing to avoiding the dissemination of PWD. [ABSTRACT FROM AUTHOR]

Copyright of International Journal of Agriculture & Wildlife Science is the property of International Journal of Agriculture & Wildlife Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Traditionally, coffee is propagated by seed. However, in recent years, the propagation of plants derived from somatic embryos has become increasingly important. The genetic homogeneity of plants derived from somatic embryos must be assessed before large-scale propagation. In this study, the genetic uniformity of somatic embryo–derived and seed-derived plants obtained from the same mother plant in two arabica hybrid coffee cultivars was examined using sequence-related amplified polymorphism (SRAP) and start codon-targeted (SCoT) markers, as well as the sequencing of seven functional genes. In both cultivars, the analysis of molecular markers revealed a remarkable genetic similarity of 93 to 98% between the plants derived from somatic embryos and those derived from seeds compared to the mother plant. Sequence analysis confirmed that the large subunit of ribulose-1,5-biphosphate carboxylase and mitochondrial ribosomal protein had the highest sequence similarity of all the genes studied. Among the five nuclear genes analyzed, the pathogenesis-related protein 1A-like gene showed the lowest sequence variability while the zinc finger protein gene showed the highest sequence variability compared to the NAC25, UDP-glycosyltransferase, and light-inducing protein chloroplastic-like genes. The presence of variable frequency of single-nucleotide polymorphisms (SNPs) and insertions and deletions (indels) created the overall sequence polymorphism across different genes. There was no substantial difference in sequence variability between plants derived from somatic embryos and those grown from seeds. This study represents the first comparative analysis of the genetic homogeneity of somatic embryo–derived and seed-derived plants of self-fertilized arabica coffee cultivars. [ABSTRACT FROM AUTHOR]

Juglans mandshurica is an important component of Korean pine mixed and secondary forests in Northeast China and is also a precious timber and economic species. In this study, plant regeneration of J. mandshurica has been established using stems with bud and mature zygotic embryos as explants. The results showed that placing the stems with buds in low-temperature conditions (0 to 4°C) and in darkness for 5 d inhibited the browning of the explants and promoted bud sprouting; Na2S2O3 also inhibited the browning of mature zygotic embryos significantly. The suitable basal media and hormones for bud sprouting and zygotic embryo germination were (1) WPM medium (woody plant medium) containing 2.0 mg·L−1 6-benzylaminopurine (6-BA) and 0.01 mg·L−1 3-indolebutyric acid (IBA) and (2) DKW medium (Driver and Kuniyuki medium) containing 2.5 mg·L−1 6-BA and 0.1 mg·L−1 IBA. The suitable basal medium and hormone for rooting of shoots from buds was 1/2 Murashige and Skoog (MS) medium (the macro-elements were half-strength of MS medium) supplemented with 1.0 mg·L−1 IBA, and the suitable basal medium and hormone for rooting of seedlings derived from zygotic embryo was 1/2 MS medium containing 1.0 mg·L−1 IBA, and DKW medium with 2.5 mg·L−1 6-BA, 0.1 mg·L−1 IBA, 0.2 mg·L−1 gibberellin A3 (GA3), and 0.2 g·L−1 activated carbon (AC). About 95% of the rooted plantlets survived after acclimation and transplantation. These results provided a foundation for the scale propagation to meet the demands of forestry production of the species. [ABSTRACT FROM AUTHOR]

In the frame of exploring the local native biodiversity for new ornamental species, the current study frames pivotal efforts for the ex situ conservation of the vulnerable and protected local Greek endemic plant Campanula laciniata L. and presents its natural requirements, seed germination trial, and first cultivation–fertilization protocol. The temperature and precipitation requirements of C. laciniata prevailing in its natural habitats were explored by using high-spatial-resolution bioclimatic maps in Geographic Information Systems (GIS). The germination of C. laciniata seeds was tested at 15 °C under alternating light and dark conditions as suggested for various Mediterranean Campanula species. However, the germination rate of C. laciniata seeds was low (35%), thus indicating the need for further research. The derived seedlings were used to study the effect of fertilization schemes on C. laciniata growth involving integrated nutrient management (INM), inorganic fertilization (ChF), and control (only water) using a substrate of soil:peat:perlite (4:3:1, v/v/v). After six months of plant growth, specific morphological and physiological characteristics as well as the phenolic content and antioxidant capacity of the plants receiving each fertilization treatment were measured. Fertilization significantly affected the morphological and physiological characteristics of the produced plants. Total phenols and antioxidant capacity were both affected by fertilization treatment but were lower in fertilized plants compared to control ones. After pivotal ex situ conservation, we performed a multifaceted evaluation for the ornamental-horticultural sector showing that C. laciniata holds a noteworthy ornamental potential (52.78%) with feasible value chain creation in the medium term for its sustainable utilization. [ABSTRACT FROM AUTHOR]

Micropropagation of fig is playing an interesting role in the rapid multiplication of varieties with important characters and the regeneration of virus-free plants. FMD (Fig Mosaic Disease) is the viral disorder the most spread all over the world and considered as a serious problem for healthy fig plant propagation and high fig production. During the last four decades, many efficient protocols have been established for in vitro large-scale propagation of female figs and caprifigs for plant material sanitation. Successful micropropagation of figs using meristematic tissues (nodal segments or apical buds) is dependant to several external and internal factors including in vitro conditions (media components essentially) and ex vitro (genotype and physiological state, etc.). Specific requirements during all steps of plant regeneration, such as the initiation of in vitro cultures, shoot multiplication, plantlet rooting and acclimatization are summarized in this review. The efficacy of meristem and tip culture in the eradication of viruses from fig vitroplants is also reported. New approaches for the improvement of shoot multiplication and fig vitroplant quality (bioreactors, Arbuscular mycorrhizal fungi, etc.) and the genetic stability and conformity of micropropagated plantlets are discussed. [ABSTRACT FROM AUTHOR]

The increasing population, its requirements for food, and the environmental impact of the excessive use of inputs make crop production a pressing challenge. Integrated nutrient management (INM) has emerged as a critical solution by maximizing nutrient availability and utilization for crops and vegetables. This review paper highlights the potential benefits of INM for various vegetables and field crops and explores the conceptual strategies, components, and principles underlying this approach. Studies have shown that a wide range of vegetables and field crops benefit from INM, in terms of increased yield and improvements in yield attributes, nutrient contents and uptake, growth parameters, and various physiological and biochemical characteristics. This paper discusses biostimulants, their categories, and their impact on plant propagation, growth, photosynthesis, seed germination, fruit set, and quality. Additionally, this review explores modern sustainable soilless production techniques such as hydroponics, aeroponics, and aquaponics. These cultivation methods highlight the advancements of controlled-environment agriculture (CEA) and its contribution to nutrient management, food security and minimizing the environmental footprint. The review concludes by proposing methods and fostering discussions on INM's future development, while acknowledging the challenges associated with its adoption. Finally, this review emphasizes the substantial evidence supporting INM as a novel and ecologically sound strategy for achieving sustainable agricultural production worldwide. [ABSTRACT FROM AUTHOR]

A successful regeneration protocol was developed for micropropagation of Lilium akkusianum R. Gämperle, an endemic species of Türkiye, from scale explants. The study also aimed to evaluate the effects of Meta-Topolin (mT) and N6-Benzyladenine (BA) on in vitro regeneration. The Murashige and Skoog medium (MS) supplemented with different levels of α-naphthaleneacetic acid (NAA)/BA and NAA/mT were used for culture initiation in the darkness. The highest callus rates were observed on explants cultured on MS medium with 2.0 mg/L NAA + 0.5 mg/L mT (83.31%), and the highest adventitious bud number per explant was 4.98 in MS medium with 0.5 mg/L NAA + 1.5 mg/L mT. Adventitious buds were excised and cultured in 16/8 h photoperiod conditions. The highest average shoot number per explant was 4.0 in MS medium with 2.0 mg/L mT + 1.0 mg/L NAA. Shoots were rooted with the highest rate (90%) in the medium with the 1.0 mg/L IBA, and the highest survival rate (87.5%) was recorded in rooted shoots in the same medium. The ISSR marker system showed that regenerated plantlets were genetically stable. Besides traditional tissue culture techniques used in the current study, the potential for improving the effectiveness of L. akkusianum propagation protocols by incorporating machine learning methodologies was evaluated. ML techniques enhance lily micropropagation by analyzing complex biological processes, merging with traditional methods. This collaborative approach validates current protocols, allowing ongoing improvements. Embracing machine learning in endemic L. akkusianum studies contributes to sustainable plant propagation, promoting conservation and responsible genetic resource utilization in agriculture. [ABSTRACT FROM AUTHOR]

Introduction: Antibiotic resistance represents one of the most significant threats to public health in the 21st century. Polyphenols, natural molecules with antibacterial activity produced by plants, are being considered as alternative antimicrobial strategies to manage infections caused by drug-resistant bacteria. In this study, we investigated the antibacterial activity of a polyphenol mixture extracted from citrus fruits, against both antibiotic-susceptible and resistant strains of Staphylococcus aureus and Staphylococcus epidermidis. Methods: Broth microdilution and time-kill curve experiments were used to test the extract anti-staphylococcal activity. Cytotoxicity was assessed by the hemolysis assay. The interaction between the mixture and antibiotics was investigated by the checkerboard assay. The effect of B alone and in combination with oxacillin on the membrane potential was investigated by the 3,3'-dipropylthiadicarbocyanine iodide assay. The ability of the extract to induce the development of resistance was verified by propagating S. aureus for 10 transfers in the presence of sub-inhibitory concentrations. Results: The citrus extract was found to be active against all Staphylococcus strains at remarkably low concentrations (0.0031 and 0.0063%), displaying rapid bactericidal effects without being toxic on erythrocytes. In particular, B was found to rapidly cause membrane depolarization. When combined with methicillin, meropenem, and oxacillin, the mixture displayed synergistic activity exclusively against methicillin-resistant strains. We additionally show that the sequential exposure of S. aureus to sub-inhibitory concentrations did not induce the development of resistance against the extract. Discussion: Overall, these findings support the potential use of the citrus extract as promising option to manage staphylococcal infections and suggest that it may counteract the mechanism behind methicillin-resistance. [ABSTRACT FROM AUTHOR]

This document discusses the role of in vitro biotechnology in the conservation and sustainable use of plant genetic resources. It highlights the increasing interest in this area to ensure an adequate food supply for future generations. The loss of biodiversity and genetic erosion of plant species are identified as significant threats, which can be addressed through biotechnological techniques such as rapid propagation and genetic improvement. The document presents various approaches for the conservation of plant genetic resources, including in vitro culture, synthetic seeds, plasma treatments, and cryopreservation. It concludes by emphasizing the need for further efforts to promote in vitro conservation and extend the application of these techniques to rare and endangered species. [Extracted from the article]

The article presents a study which established a method for the propagation of Eulophia nuda Lindl, an endangered species belonging to the Orchidaceae family which has vast potential medicinal applications, through seed germination in vitro and embryogenesis. Topics include sterilization of the explant and inoculation, effect of plant growth regulators (PGRs) on embryogenesis, and effect of different media on seed germination and embryo formation.

Under laboratory conditions, segments of potato with single nodes were exposed to varying doses of Sodium Chloride (NaCl) (0 mM, 20 mM, 40 mM, 60 mM, 80 mM and 100 mM), using the Murashige and Skoog growth medium to assess how NaCl salt stress affects micropropagation, callus formation, and regeneration in the 'Desiree' potato plant cultivar, and also seeks to determine the ability of this cultivar to thrive in salt stress conditions. The data were collected after a six-week period for each salt treatment. Remarkably, a significant increase in the mass of green and dry stems and roots was observed specifically under the 40 mM NaCl treatment. Conversely, the length of shoots and branches experienced a reduction as the NaCl concentration increased. The overall impact of the NaCl treatments strongly influenced root weight. Concerning the formation (development) and revitalization of callus, segments of potato microtubers were exposed to the above-mentioned NaCl concentrations, resulting in an intermediate level of salt stress that significantly reduced callus weights as NaCl concentration increased. Furthermore, a gradual decrease in the regeneration rate was noted with increasing concentrations of the plant growth regulator BA (1 to 4 mg/l). The most profound relative regeneration rate occurred at 1 mg/l BA. Notably, the counteractive effect of salt was more apparent with higher NaCl concentrations, with exceptions at 20 mM and 40 mM. These findings propose that the 'Desiree' potato cultivar exhibits moderate tolerance to salt stress and indicates a capacity to endure salinity. Moreover, this valuable variety could potentially be harnessed through genetic manipulation to enhance its salt resilience. [ABSTRACT FROM AUTHOR]

The significant issue of managing in-vitro culture browning in plant tissue culture procedures is explored in depth in this review. The complex issue of browning, which includes oxidative, enzymatic, and non-enzymatic browning, poses obstacles to the effectiveness of in vitro culture and has an impact on the quality of secondary metabolites as well as plant viability. Researchers and practitioners encounter this issue in their pursuit of successful plant propagation and valuable secondary metabolite production. The review explores a range of control measures, including antioxidants like ascorbic acid, polyvinylpyrrolidone (PVP), and potassium citrate, alterations to the culture medium, and the selection of suitable culture vessels. These techniques provide a comprehensive method for controlling browning and getting good results in plant tissue culture. The knowledge gathered from this research advances the industry and promotes sustainability since plant tissue culture is still essential for creating disease-free plants, quickly proliferating uncommon genotypes, and synthesizing useful plant-derived compounds. Whether enhancing crop traits or cultivating plants with economically significant products, effective control of culture browning remains a key factor in achieving these goals. In conclusion, this review advances our understanding of the multifaceted challenge of culture browning in plant tissue culture and provides actionable solutions to address this issue. By implementing these measures, researchers and practitioners can enhance the outcomes of plant tissue culture, maximize the yield of valuable products, and contribute to the growth and sustainability of agriculture and industry. [ABSTRACT FROM AUTHOR]

In this study, we refine in vitro propagation techniques for Camellia sinensis using a machine learning approach to ascertain the influence of different shooting and rooting conditions on key growth metrics. This was achieved by applying random forest (RF), XGBoost, and multilayer perceptron (MLP) models to dissect the complexities of micropropagation and rooting processes. The research unveiled significant disparities in growth metrics under varying media conditions, underscoring the profound impact of media composition on plant development. The meticulous statistical analysis, employing ANOVA, highlighted statistically significant differences in growth metrics, indicating the critical role of media composition in optimizing growth conditions. Methodologically, the study utilized explants from 2–3-year-old tea plants, which underwent sterilization before being introduced to two distinct culture media for their micropropagation and rooting phases. Statistical analyses were conducted to evaluate the differences in growth outcomes between media, while machine learning models were employed to predict the efficacy of micropropagation and rooting based on various growth regulators. This approach allowed for a comprehensive evaluation of the model's performance in simulating plant growth under different conditions, leveraging metrics like R2, RMSE, and MAE. The findings from this study significantly advance the understanding of tea plant micropropagation, highlighting the utility of machine learning models in agricultural optimization. This research contributes to enhancing micropropagation strategies for the tea plant and exemplifies the transformative potential of integrating machine learning into plant science, paving the way for improved agricultural and horticultural practices. This interdisciplinary approach offers a novel perspective on optimizing in vitro propagation processes, contributing substantially to plant tissue culture and biotechnology. [ABSTRACT FROM AUTHOR]

Protected geographical indications (PGIs) share health problems related to plant propagation material. The PGI "Faba de Lourenzá" encompasses a 1660 km2 area in northern Galicia, Spain, renowned for its "Faba Galaica" (FG) and Faba do marisco" (FM) bean cultivars. The lack of certified virus-free seeds poses a challenge. From 2019 to 2023, seeds from 60 lots were tested for BCMV. Plants from several plots were tested periodically to develop disease progress curves (DPCs). Control methods (plots out PGI zone, virus-free seedlings, rogueing, corn borders, and intercropping) were tested. Yields in five plots were used to assess BCMV's economic impact. Seed lots were 22.3% FG-infected and <5% FM-infected. The transmission rate of BCMV from infected FG plants to their seeds was 25.5 ± 5%, while for FM it was 12 ± 3%. FG yield losses were on average 31.6 ± 4.5%. Combining virus-free seedlings and infected plant removal in plots outside the PGI area proved effective at reducing infection rates; combining with intercropping resulted in the lowest incidence in an FG plot. Farmer training and off-site plot selection to produce healthy sowing beans are key to improving results. [ABSTRACT FROM AUTHOR]

Since prehistoric times, medicinal and aromatic plants (MAPs) have been employed for various therapeutic purposes due to their varied array of pharmaceutically relevant bioactive compounds, i.e. secondary metabolites. However, when secondary metabolites are isolated directly from MAPs, there is occasionally very poor yield and limited synthesis of secondary metabolites from particular tissues and certain developmental stages. Moreover, many MAPs species are in danger of extinction, especially those used in pharmaceuticals, as their natural populations are under pressure from overharvesting due to the excess demand for plant‐based herbal remedies. The extensive use of these metabolites in a number of industrial and pharmaceutical industries has prompted a call for more research into increasing the output via optimization of large‐scale production using plant tissue culture techniques. The potential of plant cells as sources of secondary metabolites can be exploited through a combination of product recovery technology research, targeted metabolite production, and in vitro culture establishment. The plant tissue culture approach provides low‐cost, sustainable, continuous, and viable secondary metabolite production that is not affected by geographic or climatic factors. This study covers recent advancements in the induction of medicinally relevant metabolites, as well as the conservation and propagation of plants by advanced tissue culture technologies. [ABSTRACT FROM AUTHOR]

The disposal of sewage sludge (SS) is an environmental issue in many large cities worldwide. Due to its high nutrients and organic matter contents, the SS can be used as a substrate to produce tree seedlings. However, few studies evaluate the suitability of SS from different wastewater treatment plants (WWTP) as seedlings' substrates. The present study assessed the chemical and physical properties of three SS from different WWTPs and two commercial substrates (CS), aiming to evaluate how the SSs' properties can affect the production of seedlings from two tree species. SS from Alegria, Ilha, and Sarapuí WWTP were assessed, and CS was composed mainly of peat and pine bark. As chemical properties, heavy metals, macro, and micronutrients total contents were evaluated. Meanwhile, density and porosity were measured as physical properties. The growth in shoot height was measured for seedlings of two Atlantic Forest species (Colubrina glandulosa and Peltophorum dubium). The SS showed higher nutrients (mainly N and P), and the CS had better physical properties. The Ilha and Sarapuí SS are recommended as substrates to produce seedlings of both species. Considering its low macroporosity, the Alegria SS must be mixed or composted with other materials to be used as a substrate for tree species. Although the contents of aluminum, zinc, and other metal were high in the SS, no adverse effects were observed in seedlings. Without chemical fertilizers, SS from Ilha and Sarapuí produced seedlings in a shorter period and with higher growth than those grown with CS plus chemical fertilizers. [ABSTRACT FROM AUTHOR]

Grafting alters the genetic and anatomical features of plants. Although grafting has been widely applied in plant propagation, the underlying processes that govern the effects of the procedure are not fully understood. Samples were collected to study the long-term influence of grafting on the leaf-shoot morphology, leaf-shoot anatomy, and genetic signature of the grafted plants. Citrulus lanatus (Thunb.) Matsum. & Nakai (cv. Lady) was used as the scion, and Lagenaria siceraria (Molina) Standl (cv. Argentario) as a rootstock. In grafted plants, leaf blades and petioles were 20.92% and 12.82% longer, respectively, while the midrib collenchyma was 35.68% thicker, and the diameter of the vessel member was 11.17% larger than in ungrafted plants. In the stem, grafting affected the arrangement and number of vascular bundles (from 1 to 2 rings). The thickness of the epidermis decreased by 69.79%, and the size of the external fascicular phloem decreased by 23.56%. The diameter of the vessel member of the grafted plants increased by 28.94%. Eight out of ten evaluated primers met the requirements (stability in both watermelons and bottle gourd, tissue-specific). In the genetic tests, we examined whether this change in the gene expression pattern is due to the grafting and, if so, to what extent. Seven out of eight tested Small Auxin Up-Regulated RNA (SAUR) genes were expressed in the ungrafted and grafted C. lanatus lines in four cases; the expression increased by more than 10% after grafting. The morpho-anatomical changes and genetic variation reported in this study for grafted lines of C. lanatus contribute to the understanding of the underlying mechanisms of plant growth observations resulting from grafting. [ABSTRACT FROM AUTHOR]

In-line digital holography is a powerful tool widely used for microscopic object imaging. Usually, in-line and out-line configurations are used to implement holographic systems, but in-line-based set-ups are preferable as they are less sensitive to mechanical vibrations and refraction index variations. However, non-desired blurred conjugate images are superposed to the reconstructed object image by using in-line systems. One strategy to remove the conjugate image contribution is to include a double-sideband filter at the Fourier plane of the system. After using the filter, data obtained at the CCD are processed to retrieve the magnitude and phase (hologram) of the diffracted wavefront while removing the conjugated image. Afterwards, a diffraction integral equation is used to digitally propagate the hologram. Despite the above-mentioned factors, there is not a thorough analysis in the literature of magnification parameters associated with the final reconstructed image, this aspect being crucial for the experimental application of the above-stated approach. Under this scenario, a theoretical analysis of the longitudinal and transverse magnifications of the reconstructed images is provided in this work. The method is validated through the simulation and experimental results of different microscopic objects: glass microspheres, a micrometric reticle, and a resolution test chart USAF 1951. The obtained results provide that the combination of magnification relations with methods for hologram propagation and optimal focused image identification is effective for object position determination. This approach could be useful for 3D microparticle localization and monitoring with optimized magnification within real-time applications. [ABSTRACT FROM AUTHOR]

Varying external conditions in the metallogenetic process of crystalline rocks contribute to the complex mineral and textural characteristics, rendering the mechanical properties highly heterogeneous at the mineral scale. This research focused on the influences of minerals with relatively low strength and stiffness (soft minerals) in crystalline rocks on their cracking behavior. A particle‐based discrete element method (DEM) was first employed to establish random grain‐based models (GBM) of crystalline rocks containing soft minerals with different contents, strengths, and stiffnesses. On this basis, responses of the mechanical properties and crack propagation to these parameters were systematically investigated and an optimized micro‐parameter calibration method for real CT (computed‐tomography) ‐based GBM was then proposed considering the characteristics of soft minerals. The results demonstrate that with the decrease of the strength of soft mica, the intragranular cracks are prone to initiate and propagate inside the soft minerals and lead to the final crack coalescence. The decrease in the stiffness of soft minerals enhances their controlling effects on the cracking propagation. Based on the CT‐based granite model, it was found that a heterogeneous stress field is produced due to the spatial distribution of the soft (mica) and hard (quartz and feldspar) minerals, and the mica minerals tend to terminate cracks or force cracks to deflect or bypass individual grains during crack propagation. This study sheds light on the damage and failure processes of crystalline rocks with contrast mineral components. [ABSTRACT FROM AUTHOR]

The domestication process in grapevines has facilitated the fixation of desired traits. Nowadays, vegetative propagation through cuttings enables easier preservation of these genotypes compared to sexual reproduction. Nonetheless, even with vegetative propagation, various phenotypes are often present within the same vineyard due to the accumulation of somatic mutations. These mutations are not the sole factors influencing phenotype. Alongside somatic variations, epigenetic variation has been proposed as a pivotal player in regulating phenotypic variability acquired during domestication. The emergence of these epialleles might have significantly influenced grapevine domestication over time. This study aims to investigate the impact of domestication on methylation patterns in cultivated grapevines. Reduced-representation bisulfite sequencing was conducted on 18 cultivated and wild accessions. Results revealed that cultivated grapevines exhibited higher methylation levels than their wild counterparts. Differential Methylation Analysis between wild and cultivated grapevines identified a total of 9955 differentially methylated cytosines, of which 78% were hypermethylated in cultivated grapevines. Functional analysis shows that core methylated genes (consistently methylated in both wild and cultivated accessions) are associated with stress response and terpenoid/isoprenoid metabolic processes. Meanwhile, genes with differential methylation are linked to protein targeting to the peroxisome, ethylene regulation, histone modifications, and defense response. Collectively, our results highlight the significant roles that epialleles may have played throughout the domestication history of grapevines. [ABSTRACT FROM AUTHOR]

In vitro mass propagation of apple plants plays an important role in the rapid multiplication of genetically uniform, disease-free scions and rootstocks with desired traits. Successful micropropagation of apple using axillary shoot cultures is influenced by several factors, the most critical of which is the cytokinin included in the culture medium. The impact of medium composition from single added cytokinins on shoot proliferation of apple scion Húsvéti rozmaring cultured on agar-agar gelled Murashige and Skoog medium fortified with indole butyric acid and gibberellic acid was investigated. The optimum concentration for efficient shoot multiplication differs according to the type of cytokinin. The highest significant multiplication rate (5.40 shoots/explant) was achieved using 2.0 μM thidiazuron while the longest shoots (1.80 cm) were observed on the medium containing benzyladenine at a concentration of 2.0 μM. However, application of either thidiazuron or benzyladenine as cytokinin source in the medium resulted in shoots of low quality, such as stunted and thickened shoots with small leaves. In the case of benzyladenine riboside, the 8 μM concentration was the most effective in increasing the multiplication rate (4.76 shoots/explant) but caused thickened stem development with tiny leaves. In the present study, meta-topolin was shown to be the most effective cytokinin that could be applied to induce sufficient multiplication (3.28 shoots/explant) and high-quality shoots along with shoot lengths of 1.46 cm when it was applied at concentrations of 4 μM. However, kinetin was the least active cytokinin; it practically did not induce the development of new shoots. The superior cytokinin for in vitro axillary shoot development of apple scion Húsvéti rozmaring with high-quality shoots was the meta-topolin, but it may be different depending on the variety/genotype under study. [ABSTRACT FROM AUTHOR]

Vanilla (Vanilla planifolia Andrews) is a valuable orchid spice cultivated for its highly priced beans. Vanilla has been planted in Hainan province of China via cutting propagation for about 40 years. The yield has been decreasing annually for the past ten years due to pod numbers declining significantly even though it seems to grow normally without disease symptoms, while the reason is still unknown. In this study, we found that Cymbidium mosaic virus (CymMV), one of the most devastating viruses causing losses in the vanilla industry, massively presented within the pods and leaves of vanilla plants, so the virus infecting the vanilla seems to be a highly probable hypothesis of the main contributions to low yield via decreasing the number of pods. This represents the first speculation of CymMV possibly affecting the yield of vanilla in China, indicating the important role of virus elimination in restoring high yield in vanilla. This research can also serve as a warning to important economic crops that rely on cuttings for propagation, demonstrating that regular virus elimination is very important for these economically propagated crops through cuttings. [ABSTRACT FROM AUTHOR]

Potato is the most important non-cereal crop worldwide, and, yet, genetic gains in potato have been traditionally delayed by the crop's biology, mostly the genetic heterozygosity of autotetraploid cultivars and the intricacies of the reproductive system. Novel site-directed genetic modification techniques provide opportunities for designing climate-smart cultivars, but they also pose new possibilities (and challenges) for breeding potato. As potato species show a remarkable reproductive diversity, and their ovules have a propensity to develop apomixis-like phenotypes, tinkering with reproductive genes in potato is opening new frontiers in potato breeding. Developing diploid varieties instead of tetraploid ones has been proposed as an alternative way to fill the gap in genetic gain, that is being achieved by using gene-edited self-compatible genotypes and inbred lines to exploit hybrid seed technology. In a similar way, modulating the formation of unreduced gametes and synthesizing apomixis in diploid or tetraploid potatoes may help to reinforce the transition to a diploid hybrid crop or enhance introgression schemes and fix highly heterozygous genotypes in tetraploid varieties. In any case, the induction of apomixis-like phenotypes will shorten the time and costs of developing new varieties by allowing the multi-generational propagation through true seeds. In this review, we summarize the current knowledge on potato reproductive phenotypes and underlying genes, discuss the advantages and disadvantages of using potato's natural variability to modulate reproductive steps during seed formation, and consider strategies to synthesize apomixis. However, before we can fully modulate the reproductive phenotypes, we need to understand the genetic basis of such diversity. Finally, we visualize an active, central role for genebanks in this endeavor by phenotyping properly genotyped genebank accessions and new introductions to provide scientists and breeders with reliable data and resources for developing innovations to exploit market opportunities. [ABSTRACT FROM AUTHOR]

BACKGROUND: One of the biggest challenges facing the soft fruit sector is to propagate disease free strawberry plants in a timely manner, financially viable approach and in an eco-friendly fashion. OBJECTIVE: This study sets out to investigate the efficacy of growing everbearing Fragaria×ananassa 'Malling Ace' runners at different propagation dates and under Different LED lighting regimes using vertical farming units and glasshouse (as control). METHODS: To evaluate the effects of the treatment factors, some of the main horticultural features including rooting success rate, rooting speed, root and shoot biomass production, chlorophyll content and bud emergence were measured and statistically analysed. In addition, this study aimed to investigate the energy efficiency of a self-sufficient Direct Current (DC) based vertical farming unit, versus a vertical farming unit using the Alternate Current (AC) electricity provided by national grid electricity. RESULTS: light, tipping date, and the interaction between light and tipping date, significantly influenced shoot biomass, total crown diameter, and chlorophyll content in the leaves of 'Malling Ace'. In terms of energy demand, the DC unit had a higher level of energy efficiency over AC unit with 23% less energy demand. CONCLUSION: In general, this trial demonstrated vertical farming as a potentially sustainable method and efficient tool in propagating strawberry plants. [ABSTRACT FROM AUTHOR]

The demand for Vaccinium fruits has skyrocketed due to their nutritional and medicinal properties, notably their high content of phenolic compounds and excellent sensory evaluation. The use of Vaccinium plants and its components as dietary supplements and health ingredients has been on the rise across the world. The attractive fruits contain a high content of anthocyanins and antioxidant compounds. In some popular and valuable cultivars, the conventional propagation methods, exploiting hard or soft wood cuttings, are inefficient. The demand for nursery plants can be fulfilled by micropropagation. This review aims to explore advances in the in vitro culture of Vaccinium plants, focusing on effective disinfection, optimized culture media, and the role of growth regulators in plant development and multiplication. By providing a controlled environment, micropropagation allows the large-scale production of these plants in a short time, ensuring availability throughout the year. Additionally, this technique offers the advantage of studying the effects of abiotic stresses on plants, as well as facilitating research on the production of relevant metabolites. This review seeks to provide an updated overview of the most promising methods and techniques for micropropagation of Vaccinium, thereby contributing to the ongoing development of the blueberry production industry and derivative products. [ABSTRACT FROM AUTHOR]