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Quantitative prediction of reservoir properties (e.g., gas saturation, porosity, and shale content) of tight reservoirs is of great significance for resource evaluation and well placements. However, the complex pore structures, poor pore connectivity, and uneven fluid distribution of tight sandstone reservoirs make the correlation between reservoir parameters and elastic properties more complicated and thus pose a major challenge in seismic reservoir characterization. We have developed a partially connected double porosity model to calculate elastic properties by considering the pore structure and connectivity, and to analyze these factors' influences on the elastic behaviors of tight sandstone reservoirs. The modeling results suggest that the bulk modulus is likely to be affected by the pore connectivity coefficient, while the shear modulus is sensitive to the volumetric fraction of stiff pores. By comparing the model predictions with the acoustic measurements of the dry and saturated quartz sandstone samples, the volumetric fraction of stiff pores and the pore connectivity coefficient can be determined. Based on the calibrated model, we have constructed a 3D rock physics template that accounts for the reservoir properties' impacts on the P-wave impedance, S-wave impedance, and density. The template combined with Bayesian inverse theory is used to quantify gas saturation, porosity, clay content, and their corresponding uncertainties from elastic parameters. The application of well-log and seismic data demonstrates that our 3D rock physics template-based probabilistic inversion approach performs well in predicting the spatial distribution of high-quality tight sandstone reservoirs in southwestern China. [ABSTRACT FROM AUTHOR]

Aiming at the shortcomings of the BP neural network in practical applications, such as easy to fall into local extremum and slow convergence speed, we optimized the initial weights and thresholds of the BP neural network using the particle swarm optimization (PSO). Additionally, cloud computing service, web technology, cloud database and numerical simulation were integrated to construct an intelligent feedback analysis cloud program for underground engineering safety monitoring based on the PSO-BP algorithm. The program could conveniently, quickly, and intelligently carry out numerical analysis of underground engineering and dynamic feedback analysis of surrounding rock parameters. The program was applied to the cloud inversion analysis of the surrounding rock parameters for the underground powerhouse of the Shuangjiangkou Hydropower Station. The calculated displacement simulated with the back-analyzed parameters matches the measured displacement very well. The posterior variance evaluation shows that the posterior error ratio is 0.045 and the small error probability is 0.999. The evaluation results indicate that the intelligent feedback analysis cloud program has high accuracy and can be applied to engineering practice. [ABSTRACT FROM AUTHOR]

To investigate the potential use of the complex function theory in displacement back analysis, a novel method for parameter identification is proposed by combining the complex function theory and the back-propagation neural network optimized by the particle swarm optimization algorithm. The finite element method is replaced with the complex function method to establish a nonlinear relationship between parameters and deformations of the surrounding rock around a complex underground cavern. As demonstrated by a virtual example of an arched tunnel, the deformation parameters were identified by the proposed method and demonstrated to be approximately equal to the predefined parameters. Subsequently, the proposed method is further applied to the inverse analysis of mechanical parameters for the surrounding rock in the underground powerhouse of the Baihetan Hydropower Station. The computed displacements based on the back-analyzed parameters show excellent agreement with the monitoring displacements. The average error between them is about 4.17%, so the proposed method provides the potential to viably enhance the back-analysis technique. [ABSTRACT FROM AUTHOR]

Porous rocks exhibit mechanical behaviors characterized by high compressibility and stiffness enhancement under quasi-static cyclic loads. In this study, we investigated the mechanical responses of a porous red sandstone subjected to different cyclic loads and revealed the mesoscopic mechanisms for the evolution of stiffness and strength. Rock stiffness was enhanced by increasing stress and number of load cycles during pre-peak loading, and the normalized viscoelastic strain evolved uniformly during all the unloading phases. Void compaction and microfracturing caused irreversible structural changes and subsequent changes in the stiffness of the rock, and there was a positive linear relationship between the unloading secant modulus and axial irreversible strain. In addition, a new parameter, named the plastic strain increment ratio, was defined to describe the asynchronous development of the axial and lateral irreversible deformation induced by void compaction and microfracturing. These results can provide a better understanding of the mechanical properties of porous sandstone. Highlights: Low-frequency cyclic loads cause stiffness enhancement on the porous red sandstone. Unloading recovery of viscoelastic strain follows a uniform exponential law regardless of loading conditions. The evolution of stiffness is linearly related to the accumulation of irreversible strains. The evolution of stiffness is caused by void compaction and microfracturing. [ABSTRACT FROM AUTHOR]

The underground powerhouse of the Shuangjiangkou hydropower station is one of the largest caverns under construction in China, and its stability during construction is crucial for safe construction. To study the stability of the surrounding rock during excavation, the displacement and stress of the surrounding rock were monitored by multi-point displacement meters and bolt stress meters. Based on the monitoring data, the elastic modulus, Poisson's ratio, friction angle, and cohesion of surrounding rock were inversely analyzed by the PSO-BP algorithm. Then, the back-analyzed parameters were used to simulate the subsequent excavations and predict the stability of surrounding rock during the following construction. The analysis results show that the surrounding rocks were generally stable during the initial four stages of excavation, and the main factors affecting their stability were blasts and unfavorable geological structures, including the lamprophyre vein and the F1 fault. These unfavorable geological structures also significantly decrease the mechanical parameters of surrounding rock as demonstrated by back analysis, and the stability prediction results show that the omnibus bar cave and the tailrace tunnel were at the greatest risk of instability during the subsequent excavations. This study provides a practical analysis for engineering excavation of the underground caverns. [ABSTRACT FROM AUTHOR]

The discrimination of river blockages is very important for the risk assessment of landslide disasters and secondary hazards. Experimental studies and statistical analyses were carried out to explore the formation process and discriminant criteria of river blockages caused by landslides. An adjustable slide chute was designed and built to conduct forty-five landslide experiments. According to the experimental results, river blockage was identified as having six types based on the differences between the water depth and the height of the landslide dam, and the degree of river blockage increased from 70% to 100% as the chute angle, particle size, and landslide volume increased. It is also found that landslide volume controls the landslide dam height and degree of blockage, and particle size and slide angle control both the landslide velocity as it enters the river and the cross-section shape of the landslide dam. To investigate more influence factors, a statistical investigation of 60 real landslide cases was carried out, and it revealed that some geometric attributes related to landslide volume have the highest correlation with river blockage, especially landslide thickness. Finally, an improved probability model was proposed to assess the possibility of complete blockage, and it has overall accuracies of 91.1% and 83.3% when applied to predict experimental landslide cases and real landslide cases, respectively. [ABSTRACT FROM AUTHOR]

Surrounding rock deformation and consequent support failure are the most prominent issues in red-bed rock tunnel engineering and are mainly caused by the effects of unloading, rheology, and swelling. This study investigated the mechanical responses of two kinds of red-bed mudstone and sandstone under unloading conditions via laboratory observation. Volume dilation was observed on the rocks during unloading, and the dilatancy stress was linear with the initial confining pressure. However, the ratios of dilatancy stress to peak stress of the two rocks kept at a range from 0.8 to 0.9, regardless of confining pressures. Both the elastic strain energy and the dissipated energy evolved synchronously with the stress–strain curve and exhibited conspicuous confining pressure dependence. Special attention was paid to the evolution behavior of the dilatancy angle. The dilatancy angle changed linearly during unloading. When the confining pressure was 10 MPa, the dilatancy angle of mudstone decreased from 26.8° to 12.5° whereas the dilatancy angle of sandstone increased from 34.6° to 51.1°; when the confining pressure rose to 25 MPa, the dilatancy angle of mudstone and sandstone decreased from 45.8° to 17.4° and increased from 21.7° to 39.5°, respectively. To further understand the evolution of the dilatancy angle, we discussed the links between the variable dilatancy angle and the processes of rock deformation and energy dissipation. [ABSTRACT FROM AUTHOR]

For high cutting slopes, it is quite necessary to set pre-reinforced structures before the slope excavation to avoid the slope failure during the excavating. In this paper, the multi-wedge translation mechanism is used to analyze the stability of high cutting slopes pre-reinforced with piles. First, the multivariate functions are established to calculate safety factor for the slope during the excavating. Based on the safety factor of the slope during the excavating process, it can be determined that whether the slope need set the pre-reinforced piles before excavating. If need, the most significant factor for pre-reinforced pile design is the stabilising force. Then the expressions are derived to calculate the stabilising force provided by the pre-reinforced piles to increase the safety factor to assure the high cutting slope is stable. At last, an example is taken to illustrate the method proposed in this study. The result shows that the safety factor for the slope is change with the excavating. The stabilising force is influenced by the location of pre-reinforced piles, excavation distance and slope angle. The most suitable location of pre-reinforced piles within the high cutting slope also had been determined. [ABSTRACT FROM AUTHOR]

The in situ stress distribution is one of the driving factors for the design and construction of underground engineering. Numerical analysis methods based on artificial neural networks are the most common and effective methods for in situ stress inversion. However, conventional algorithms often have some drawbacks, such as slow convergence, overfitting, and the local minimum problem, which will directly affect the inversion results. An intelligent inverse method optimizing the back-propagation (BP) neural network with the particle swarm optimization algorithm (PSO) is applied to the back analysis of in situ stress. The PSO algorithm is used to optimize the initial parameters of the BP neural network, improving the stability and accuracy of the inversion results. The numerical simulation is utilized to calculate the stress field and generate training samples. In the application of the Shuangjiangkou Hydropower Station underground powerhouse, the average relative error decreases by about 3.45% by using the proposed method compared with the BP method. Subsequently, the in situ stress distribution shows the significant tectonic movement of the surrounding rock, with the first principal stress value of 20 to 26 MPa. The fault and the lamprophyre significantly influence the in situ stress, with 15–30% localized stress reduction in the rock mass within 10 m. The research results demonstrate the reliability and improvement of the proposed method and provide a reference for similar underground engineering. [ABSTRACT FROM AUTHOR]

Landslides are usually caused by rainstorms and geological processes such as earthquakes and may have a massive impact on human production and life. The hazard chain of landslide–river blockage–outburst flood is the most common hazard chain caused by landslides. A database based on existing landslide cases was established in this study to investigate the assessment formulas of the risk of river blockage, dam stability, and peak flood discharge after a dam has broken. A risk assessment model of the landslide–river blockage–breaching hazard chain was established, including the downstream vulnerability. The case of the Baige landslide verified the applicability of the model. This model can be used in a landslide-prone area to predict whether a relatively massive river blockage will form after the landslide occurs, whether the landslide dam formed by the river blockage will breach in a short time, and the impact of the outburst flood on the downstream area. [ABSTRACT FROM AUTHOR]

Intervertebral disc (IVD) degeneration and its inflammatory microenvironment can result in discogenic pain, which has been shown to stem from the nucleus pulposus (NP). Increasing evidence suggests that mitochondrial related genes are strictly connected to cell functionality and, importantly, it can regulate cell immune activity in response to damaged associated signals. Therefore, identification of mitochondria related genes might offer new diagnostic markers and therapeutic targets for IVD degeneration. In this study, we identified key genes involved in NP tissue immune cell infiltration during IVD degeneration by bioinformatic analysis. The key modules were screened by weighted gene co-expression network analysis (WCGNA). Characteristic genes were identified by random forest analysis. Then gene set enrichment analysis (GSEA) was used to explore the signaling pathways associated with the signature genes. Subsequently, CIBERSORT was used to classify the infiltration of immune cells. Function of the hub gene was confirmed by PCR, Western blotting and ELISA. Finally, we identified MFN2 as a crucial molecule in the process of NP cell pyroptosis and NLRP3 inflammasome activation. We speculate that the increased MFN2 expression in NP tissue along with the infiltration of CD8+ T cells, NK cell and neutrophils play important roles in the pathogenesis of IVD degeneration. [ABSTRACT FROM AUTHOR]

Sea ice leads are elongated fractures within sea ice cover, playing an important role in the heat exchange from the ocean to the overlying atmosphere. Narrow leads less than a hundred meters in width contribute considerable heat fluxes, requiring fine-scale observation of Arctic leads. With the launch of Sustainable Development Science Satellite 1 (SDGSAT-1) by China on 5 November 2021, the on-board Thermal Infrared Spectrometer (TIS) provides thermal infrared imagery at an unprecedented resolution of 30 m in a swath of 300 km. We propose a method adapted to the TIS high-resolution infrared images for lead detection in the Arctic. For the first time, the spatial resolution of leads by infrared remote sensing increases from the scale of kilometers to tens of meters. For the Beaufort Sea cases in April 2022, the detection is consistent with the Sentinel-2 visible images, yielding an overall accuracy of 96.30%. Compared with the Moderate-Resolution Imaging Spectroradiometer (MODIS), the TIS presents more leads with width less hundreds of meters than the results based on the MODIS data. For the three infrared bands of the TIS, the B2 (10.3-11.3 µm) and B3 (11.5-12.5 µm) bands, show similar performances in detecting leads. The B1 band (8.0-10.5 µm) can be complementary to the other two bands, as the temperature measurement sensitivity is different from the other two, benefiting better detection by combining the three bands. This study demonstrates that SDGSAT-1 TIS data at 30 m resolution is well applicable for observing previously unresolvable ice leads, and will provide insight into the contribution of narrow leads to rapid sea ice changes in the Arctic. [ABSTRACT FROM AUTHOR]

Auxin response factors (ARFs) are transcription factors that play important roles in plants. ARF2 is a member of the ARF family and participates in many plant growth and developmental processes. However, the role of ARF2 in strawberry fruit quality remains unclear. In this study, FveARF2 was isolated from the woodland strawberry ‘Ruegen’ using reverse transcription-polymerase chain reaction (RT-PCR), which showed that FveARF2 expression levels were higher in the stem than in other organs of the ‘Ruegen’ strawberry. Moreover, FaARF2 was higher in the white fruit stage of cultivated strawberry fruit than in other stage. Subcellular localization analysis showed that FveARF2 is located in the nucleus, while transcriptional activation assays showed that FveARF2 inhibited transcription in yeast. Silencing FveARF2 in cultivated strawberry fruit revealed earlier coloration and higher soluble solid, sugar, and anthocyanin content in the transgenic fruit than in the control fruit, overexpression of FveARF2 in strawberry fruit delayed ripening and lower soluble solid, sugar, and anthocyanin content compared to the control fruit. Gene expression analysis indicated that the transcription levels of the fruit ripening genes FaSUT1, FaOMT, and FaCHS increased in FveARF2-RNAi fruit and decreased in FveARF2-OE fruit, when compared with the control. Furthermore, yeast onehybrid (Y1H) and GUS activity experiments showed that FveARF2 can directly bind to the AuxRE (TGTCTC) element in the FaSUT1, FaOMT, and FaCHS promoters in vitro and in vivo. Potassium ion supplementation improved the quality of strawberry fruit, while silencing FveARF2 increased potassium ion content in transgenic fruit. The Y1H and GUS activity experiments also confirmed that FveARF2 could directly bind to the promoter of FveKT12, a potassium transporter gene, and inhibited its expression. Taken together, we found that FveARF2 can negatively regulate strawberry fruit ripening and quality, which provides new insight for further study of the molecular mechanism of strawberry fruit ripening. [ABSTRACT FROM AUTHOR]

We report an experimental study of the Prandtl-number effects in quasi-two-dimensional (quasi-2-D) Rayleigh-Bénard convection. The experiments were conducted in four rectangular convection cells over the Prandtl-number range of 11.7 Pr 650.7 and over the Rayleigh-number range of 6.0 × 108 Ra 3.0 × 1010. Flow visualization reveals that, as Pr increases from 11.7 to 145.7, thermal plumes pass through the central region much less frequently and their self-organized large-scale motion is more confined along the periphery of the convection cell. The large-scale flow is found to break down for higher Pr, resulting in a regime transition in the Reynolds number Re. For the Pr range with a large-scale flow of system size, the Re number, Nusselt number Nu and local temperature fluctuations were investigated systematically. It is found that Re scales as Re ∼ Ra0.58Pr-0.82 in the present geometry, which suggests that it is in line with the behaviour in the 2-D configuration. On the other hand, the measured Nu(Ra, Pr) relation Nu ∼ Ra0.289Pr-0.02 tends to be compatible with the finding in a three-dimensional (3-D) system. For the temperature fluctuations in the cell centre and near the sidewall, they exhibit distinct Ra-dependent scalings that could not be accounted for with existing theories, but their Pr dependences for Pr 50 are in agreement with the predictions by Grossmann & Lohse (Phys. Fluids, vol. 16, 2004, pp. 4462-4472). These results enrich our understanding of quasi-2-D thermal convection, and its similarities and differences compared to 2-D and 3-D systems. [ABSTRACT FROM AUTHOR]

Two mu-zero resonance (MZR) resonators are etched in the patch of a microstrip antenna. The two MZR resonators generate two new resonances. As the MZR resonances are lower than the microstrip antenna resonance and the resonances merge with each other, size reduction and bandwidth enhancement were obtained. A prototype was designed and measured. The measured impedance bandwidth increased from 640 MHz (5.31-5.95 GHz, 11.33%) of the referenced microstrip antenna (RMA) to 940 MHz (4.99-5.93 GHz, 17.22%) of the proposed MZR loaded microstrip antenna (MZRMA). Moreover, the patch size is decreased from 0.354λl × 0.283λl of the RMA to 0.332λl × 0.266λl of the MZR-MA, and unidirectional radiation patterns are obtained for the microstrip patch and MZR resonances. A microstrip line based model was built to analyze the MZR resonators. [ABSTRACT FROM AUTHOR]

With the increased frequency of shipping activities, navigation safety has become a major concern, especially when economic losses, human casualties and environmental issues are considered. As a contributing factor, the sea state plays a significant role in shipping safety. However, the types of dangerous sea states that trigger serious shipping accidents are not well understood. To address this issue, we analyzed the sea state characteristics during ship accidents that occurred in poor weather or heavy seas based on a 10-year ship accident dataset. Sea state parameters of a numerical wave model, i.e., significant wave height, mean wave period and mean wave direction, were analyzed for the selected ship accident cases. The results indicated that complex sea states with the co-occurrence of wind sea and swell conditions represent threats to sailing vessels, especially when these conditions include similar wave periods and oblique wave directions. [ABSTRACT FROM AUTHOR]

CD4+ T-cell differentiation plays an important role in allergic airway diseases. Tumor necrosis factor receptor 2 (TNFR2) has been shown to regulate CD4+ T-lymphocyte differentiation, but its role in allergic airway inflammation is not clear. Here, we investigated the role of TNFR2 in allergic airway inflammation. The mouse model was generated by immunization with ovalbumin and intranasal administration of TNFR2 antibody. Airway inflammation and CD4+ T-cell differentiation were measured in vivo and in vitro. Inhibited TNFR2 signaling aggravated airway inflammation and increased the expression of inflammatory cytokines (IL-4, IL-5, IL-17, and TNF-α) in serum and bronchoalveolar lavage fluid. Impaired TNFR2 signaling promoted Th2 and Th17 polarization but inhibited Th1 and CD4+CD25+ T-cell differentiation in vivo. Furthermore, TNFR2 signaling inhibition promoted Th2 and Th17 polarization in vitro, which may occur through the activation of TNF receptor-associated factor 2 and NF-κB signaling. Therefore, our findings indicate that impaired TNF/TNFR2 signaling enhances Th2 and Th17 polarization and aggravates allergic airway inflammation. [ABSTRACT FROM AUTHOR]

Medium spiny neurons (MSNs), the major GABAergic projection neurons in the striatum, are implicated in many neuropsychiatric diseases such as schizophrenia, but the underlying mechanisms remain unclear. We found that a deficiency in Erbb4, a schizophrenia risk gene, inMSNs of the nucleus accumbens (NAc) core, but not the dorsomedial striatum, markedly induced schizophrenia-like behaviors such as hyperactivity, abnormal marble-burying behavior, damaged social novelty recognition, and impaired sensorimotor gating function in male mice. Using immunohistochemistry, Western blot, RNA interference, electrophysiology, and behavior test studies, we found that these phenomena were mediatedbyincreasedGABAA receptorα 1subunit(GABAARα1) expression, whichenhancedinhibitory synaptictransmissiononMSNs. These results suggest that Erbb4 in MSNs of the NAc core may contribute to the pathogenesis of schizophrenia by regulating GABAergic transmission and raise the possibility that GABAARα1 may therefore serve as a new therapeutic target for schizophrenia. [ABSTRACT FROM AUTHOR]

Three new indolediterpenoids, namely, 22-hydroxylshearinine F (1), 6-hydroxylpaspalinine (2), and 7-O-acetylemindole SB (3), along with eight related known analogs (4-11), were isolated from the sea-anemone-derived fungus Penicillium sp. AS-79. The structures and relative configurations of these compounds were determined by a detailed interpretation of the spectroscopic data, and their absolute configurations were determined by ECD calculations (1 and 2) and single-crystal X-ray diffraction (3). Some of these compounds exhibited prominent activity against aquatic and human pathogenic microbes. [ABSTRACT FROM AUTHOR]

With the increased frequency of shipping activities, navigation safety has become a major concern, especially when economic losses, human casualties and environmental issues are considered. As a contributing factor, sea state conditions play a significant role in shipping safety. However, the types of dangerous sea states that trigger serious shipping accidents are not well understood. To address this issue, we analyzed the sea state characteristics during ship accidents that occurred in poor weather or heavy seas based on a ten-year ship accident dataset. The sea state parameters, including the significant wave height, the mean wave period and the mean wave direction, obtained from numerical wave model data were analyzed for selected ship accidents. The results indicated that complex sea states with the co-occurrence of wind sea and swell conditions represent threats to sailing vessels, especially when these conditions include close wave periods and oblique wave directions. [ABSTRACT FROM AUTHOR]

De novo shoot regeneration is widely used in fundamental studies and agricultural applications. Actin microfilaments are involved in many aspects of plant cell division, cell morphogenesis and cell signal transduction. However, the function of actin microfilaments during de novo shoot regeneration is poorly understood. Here, we investigated the organization of actin microfilaments during this process and found that stem cell formation was associated with microfilament depolymerization. Furthermore, inhibition of microfilament depolymerization by phalloidin treatment or downregulation of actin depolymerizing factors (ADFs) restrained stem cell initiation and shoot regeneration. Inhibition of ADF expression affected the architecture of microfilaments during stem cell formation, and the polar transport and distribution of auxin were also disrupted. Together, our results demonstrate that organization of the microfilament cytoskeleton play important roles in stem cell formation and shoot meristem induction during shoot regeneration. [ABSTRACT FROM AUTHOR]

Four new indolediketopiperazine derivatives (1-4), along with nine known congeners (5-13), were isolated and identified from the culture extract of Eurotium cristatum EN-220, an endophytic fungus obtained from the marine alga Sargassum thunbergii. The structures of thesecompounds were elucidated on the basis of extensive spectroscopic analysis and the absolute configurations of compounds 1-4 were established by NOESY experiments and by chiral HPLC analyses of their acid hydrolysates. The absolute configuration of C-8 (a quaternary carbon substituted with a hydroxyl group) in 5 of preechinulin class was firstly determined by electronic circular dichroism (ECD) calculations. All these compounds were evaluatedfor brine shrimp (Artemia salina) lethality and nematicidal activity as well as antioxidativeand antimicrobial potency. [ABSTRACT FROM AUTHOR]

Differentiated embryo chondrocyte expressed gene 1 (Dec1), a crucial cell differentiation mediator and apoptosis inhibitor, is abundantly expressed in various types of human cancer and is associated with malignant tumor progression. As poor differentiation and low apoptosis are closely associated with poor survival rates and a poor response to radio/chemotherapy in patients with cancer, the prognostic value of Dec1 expression was examined in the present study and its correlation with response to temozolomide (TMZ) chemotherapy was analyzed in patients with glioma. Dec1 expression was analyzed by immunohistochemistry in 157 samples of newly diagnosed glioma and 63 recurrent glioblastoma cases that relapsed during TMZ chemotherapy. Correlations with clinical variables, prognosis and the response to TMZ chemotherapy were analyzed in the newly diagnosed gliomas. Dec1 expression was also compared with the apoptosis index determined by TdT-mediated dUTP nick ending-labeling assay in recurrent glioblastomas. The antiglioma effect of TMZ in nude mice xenografts with Dec1 expression was examined in vivo. High expression of Dec1, which was significantly associated with high pathological tumor grade and poor response to TMZ chemotherapy, was demonstrated to be an unfavorable independent prognostic factor and predicted poor survival in patients with newly diagnosed glioma. In patients with recurrent glioblastoma, there was a negative correlation between Dec1 expression and the apoptotic index. In nude mice treated with TMZ, Dec1 overexpression potentiated proliferation, but attenuated TMZ-induced apoptosis. In conclusion, Dec1 is a prognostic factor for the clinical outcome and a predictive factor for the response to TMZ chemotherapy in patients with glioma. [ABSTRACT FROM AUTHOR]

Two new diphenylketones (1 and 2), a new xanthone (3), and a known xanthone analogue (4) were isolated and identified from Talaromyces islandicus EN-501, an endophytic fungus obtained from the fresh collected marine red alga Laurencia okamurai. Their structures were elucidated on the basis of NMR spectroscopic and X-ray crystallographic analysis. The joint isolation of benzophenones and xanthones from the same fungal strain supports the biogenesis of xanthones via a benzophenone intermediate. It is worth mentioning that xanthones 3 and 4 have a methyl group at C-6 and C-2, respectively, which is uncommon compared with typical xanthones usually having a methyl group at C-8. Compounds 1-4 exhibited potent antioxidative activities against DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonate) radicals with IC50 values ranging from 0.58 to 6.92 μg/mL, which are stronger than that of the positive controls BHT (butylated hydroxytoluene) and ascorbic acid. Compounds 1, 3, and 4 also showed inhibitory activities against several pathogenic bacteria. [ABSTRACT FROM AUTHOR]

Purpose: The purpose of this study is to evaluate the therapeutic efficacy and safety of stem cells for the treatment of patients with ST-segment elevation myocardial infarction (STEMI).Materials and Methods: We performed a systematic review and meta-analysis of relevant published clinical studies. A computerized search was conducted for randomized controlled trials of stem cell therapy for STEMI.Results: Twenty-eight randomized controlled trials with a total of 1,938 STEMI patients were included in the present meta-analysis. Stem cell therapy resulted in an improvement in long-term (12 months) left ventricular ejection fraction of 3.15% (95% confidence interval 1.01-5.29, P<0.01). The 3-month to 4-month, 6-month, and 12-month left ventricular end-systolic volume showed favorable results in the stem cell therapy group compared with the control group (P≤0.05). Significant decrease was also observed in left ventricular end-diastolic volume after 3-month to 4-month and 12-month follow-up compared with controls (P<0.05). Wall mean score index was reduced significantly in stem cell therapy group when compared with the control group at 6-month and 12-month follow-up (P=0.01). Moreover, our analysis showed a significant change of 12-month infarct size decrease in STEMI patients treated with stem cells compared with controls (P<0.01). In addition, no significant difference was found between treatment group and control in adverse reactions (P>0.05).Conclusion: Overall, stem cell therapy is efficacious in the treatment of patients with STEMI, with low rates of adverse events compared with control group patients. [ABSTRACT FROM AUTHOR]

Cryptochromes (CRY) are blue-light photoreceptors that mediate various light responses in plants and animals. It has long been demonstrated that Arabidopsis CRY (CRY1 and CRY2) C termini (CCT1 and CCT2) mediate light signaling through direct interaction with COP1. Most recently, CRY1 N terminus (CNT1) has been found to be involved in CRY1 signaling independent of CCT1, and implicated in the inhibition of gibberellin acids (GA)/brassinosteroids (BR)/auxin-responsive gene expression. Here, we performed RNA-Seq assay using transgenic plants expressing CCT1 fused to β-glucuronidase (GUS-CCT1, abbreviated as CCT1), which exhibit a constitutively photomorphogenic phenotype, and compared the results with those obtained previously from cry1cry2 mutant and the transgenic plants expressing CNT1 fused to nuclear localization signal sequence (NLS)-tagged YFP (CNT1-NLS-YFP, abbreviated as CNT1), which display enhanced responsiveness to blue light. We found that 2903 (67.85%) of the CRY-regulated genes are regulated by CCT1 and that 1095 of these CCT1-regulated genes are also regulated by CNT1. After annotating the gene functions, we found that CCT1 is involved in mediating CRY1 regulation of phytohormone-responsive genes, like CNT1, and that about half of the up-regulated genes by GA/BR/auxin are down-regulated by CCT1 and CNT1, consistent with the antagonistic role for CRY1 and these phytohormones in regulating hypocotyl elongation. Physiological studies showed that both CCT1 and CNT1 are likely involved in mediating CRY1 reduction of seedlings sensitivity to GA under blue light. Furthermore, protein expression studies demonstrate that the inhibition of GA promotion of HY5 degradation by CRY1 is likely mediated by CCT1, but not by CNT1. These results give genome-wide transcriptome information concerning the signaling mechanism of CRY1, unraveling possible involvement of its C and N termini in its regulation of response of GA and likely other phytohormones. [ABSTRACT FROM AUTHOR]

Background: Cancer pain, especially the one caused by metastasis in bones, is a severe type of pain. Pain becomes chronic unless its causes and consequences are resolved. With improvements in cancer detection and survival among patients, pain has been considered as a great challenge because traditional therapies are partially effective in terms of providing relief. Cancer pain mechanisms are more poorly understood than neuropathic and inflammatory pain states. Chronic inflammatory pain and neuropathic pain are influenced by NB001, an adenylyl cyclase 1 (AC1)-specific inhibitor with analgesic effects. In this study, the analgesic effects of NB001 on cancer pain were evaluated. Results: Pain was induced by injecting osteolytic murine sarcoma cell NCTC 2472 into the intramedullary cavity of the femur of mice. The mice injected with sarcoma cells for four weeks exhibited significant spontaneous pain behavior and mechanical allodynia. The continuous systemic application of NB001 (30 mg/kg, intraperitoneally, twice daily for three days) markedly decreased the number of spontaneous lifting but increased the mechanical paw withdrawal threshold. NB001 decreased the concentrations of cAMP and the levels of GluN2A, GluN2B, p-GluA1 (831), and p-GluA1 (845) in the anterior cingulate cortex, and inhibited the frequency of presynaptic neurotransmitter release in the anterior cingulate cortex of the mouse models. Conclusions: NB001 may serve as a novel analgesic to treat bone cancer pain. Its analgesic effect is at least partially due to the inhibition of AC1 in anterior cingulate cortex. [ABSTRACT FROM AUTHOR]

Derivation of sea surface wind direction is a key step of sea surface wind retrieval from spaceborne Synthetic Aperture Radar (SAR) data. This technical note describes an implementation of the Local Gradient (LG) method to derive sea surface wind directions at a scale of a few kilometers from X-band spaceborne SAR TerraSAR-X (TS-X) data in wide swath mode. The 180° ambiguity in the derived sea surface wind direction is automatically eliminated using a single reference wind direction from external data sources. Several typical cases acquired in the North Sea were presented to demonstrate the derivation of sea surface wind direction under different wind situations using this method. The derived sea surface wind direction were further compared to atmospheric model prediction results. In addition, a practical method is introduced to address ambiguity in the derived sea surface wind directions using the LG method in a typhoon case with rotating surface wind structure. By interpolating the derived wind directions at a scale of kilometers, sea surface wind speeds with a spatial resolution of 500 m are subsequently retrieved using the X-band SAR sea surface wind Geophysical Model Function (GMF). The approach accomplished by combining the LG method with the X-band GMF for deriving sea surface wind in high spatial resolution demonstrates its potential for operational service. [ABSTRACT FROM AUTHOR]

Paecilomyces hepiali is a species of fungus isolated from a field collection of natural Cordyceps sinensis insect-fungi complex from the Baima Snow Mountains, Diqing Tibetan Autonomous Prefecture, Yunnan Province. A dried culture of living strain 82-2 was designated as the holotype and deposited in the Herbarium of the Institute of Chinese Materia Medica (HICMM), China Academy of Chinese Medical Sciences, Beijing. In 2015 a neotype was designated for P. hepiali based on the presumption that the holotype was unavailable for study. We show that the holotype is extant and available, and thus the neotypification is overturned. [ABSTRACT FROM AUTHOR]

Background: Since chemosensory genes play key roles in insect behaviour, they can potentially be used as new targets for pest control. The cabbage beetle, Colaphellus bowringi, is a serious insect pest of cruciferous vegetables in China and other Asian countries. However, a systematic identification of the chemosensory genes expressed in the antennae has not been reported. Results: We assembled the antennal transcriptome of C. bowringi by using Illumina sequencing technology and identified 104 candidate chemosensory genes by analyzing transcriptomic data, which included transcripts encoding 26 odorant-binding proteins (OBPs), 12 chemosensory proteins (CSPs), four sensory neuron membrane proteins (SNMPs), 43 odorant receptors (ORs), nine ionotropic receptors (IRs), and ten gustatory receptors (GRs). The data obtained are similar to those found in other coleopteran species, suggesting that our approach successfully identified the chemosensory genes of C. bowringi. The expression patterns of 43 OR genes, some of which were predominately found in the antenna or associated with sex-biased expression, were analyzed using quantitative real time RT-PCR (qPCR). Conclusions: Our study revealed that a large number of chemosensory genes are expressed in C. bowringi. These candidate chemosensory genes and their expression profiles in various tissues provide further information on understanding their function in C. bowringi as well as other insects, and identifying potential targets to disrupt the odorant system in C. bowringi so that new methods for pest management can be developed. [ABSTRACT FROM AUTHOR]

We present experimental evidence that a minute amount of polymer additives can significantly enhance heat transport in the bulk region of turbulent thermal convection. The effects of polymer additives are found to be the enhancement of coherent heat fluxes and suppression of incoherent heat fluxes. The enhanced heat transport is associated with the increased coherency of thermal plumes, as a result of the suppression of small-scale turbulent fluctuations by polymers. The incoherent heat flux, arising from turbulent background fluctuations, makes no net contribution to heat transport. The fact that polymer additives can increase the coherency of thermal plumes is supported by the measurements of a number of local quantities, such as the extracted plume amplitude and width, the velocity autocorrelation functions and the velocity-temperature cross-correlation coefficient. The results from local measurements also suggest the existence of a threshold value for the polymer concentration, only above which significant modification of the plume coherent properties and enhancement of the local heat flux can be observed. Estimation of the plume emission rate suggests a stabilization of the thermal boundary layer by polymer additives. [ABSTRACT FROM AUTHOR]

The potential anti-tumor agent wentilactones were produced by a newly isolated marine fungus Aspergillus dimorphicus. This fungus was derived from deep-sea sediment and identified by polyphasic approach, combining phenotypic, molecular, and extrolite profiles. However, wentilactone production was detected only under static cultures with very low yields. In order to improve wentilactone production, culture conditions were optimized using the response surface methodology. Under the optimal static fermentation conditions, the experimental values were closely consistent with the prediction model. The yields of wentilactone A and B were increased about 11-fold to 13.4 and 6.5 mg/L, respectively. The result was further verified by fermentation scale-up for wentilactone production. Moreover, some small-molecule elicitors were found to have capacity of stimulating wentilactone production. To our knowledge, this is first report of optimized production of tetranorlabdane diterpenoids by a deep-sea derived marine fungus. The present study might be valuable for efficient production of wentilactones and fundamental investigation of the anti-tumor mechanism of norditerpenoids. [ABSTRACT FROM AUTHOR]

Peniciadametizine A (1); a new dithiodiketopiperazine derivative possessing a unique spiro[furan-2,7'-pyrazino[1,2-b][1,2]oxazine] skeleton, together with a highly oxygenated new analogue, peniciadametizine B (2); as well as two known compounds, brasiliamide A (3); and viridicatumtoxin (4), were isolated and identified from Penicillium adametzioides AS-53, a fungus obtained from an unidentified marine sponge. The unambiguous assignment of the relative and absolute configuration for the spiro center C-2 of compound 1 was solved by the combination of NMR and ECD measurements with Density-Functional Theory (DFT) conformational analysis and Time-Dependent Density-Functional Theory-Electronic Circular Dichroism (TDDFT-ECD) calculations. The spiro[furan-2,7'-pyrazino[1,2-b][1,2]oxazine] skeleton of 1 has not been reported yet among natural products and the biosynthetic pathway for 1 and 2 was discussed. Compounds 1 and 2 showed inhibitory activity against the pathogenic fungus Alternaria brassicae. [ABSTRACT FROM AUTHOR]

The Geophysical Model Function (GMF) XMOD1 provides a linear algorithm for sea surface wind field retrievals for the Spaceborne Imaging Radar-C/X-band Synthetic Aperture Radar (SIR-C/X-SAR). However, the relationship between the normalized radar cross section (NRCS) and the sea surface wind speed, wind direction and incidence angles is non-linear. Therefore, in this paper, XMOD1 is revisited using the full dataset of X-SAR acquired over the ocean. We analyze the detailed relationship between the X-SAR NRCS, incidence angle and sea surface wind speed. Based on the C-band GMF CMOD_IFR2, an updated empirical retrieval model of the sea surface wind field called SIRX-MOD is derived. In situ buoy measurements and the scatterometer data of ERS-1/SCAT are used to validate the retrieved sea surface wind speeds from the X-SAR data with SIRX-MOD, which respectively yield biases of 0.13 m/s and 0.16 m/s and root mean square (RMS) errors of 1.83 m/s and 1.63 m/s. [ABSTRACT FROM AUTHOR]

Microglia are the resident immune cells in the CNS and play diverse roles in the maintenance of CNS homeostasis. Recent studies have shown that microglia continually survey the CNS microenvironment and scavenge cell debris and aberrant proteins by phagocytosis and pinocytosis, and that reactive microglia are capable to present antigens to T cells and initiate immune responses. However, how microglia process the endocytosed contents and evoke an immune response remain unclear. Here we report that a size-dependent selective transport of small soluble contents from the pinosomal lumen into lysosomes is critical for the antigen processing in microglia. Using fluorescent probes and water-soluble magnetic nanobeads of defined sizes, we showed in cultured rodent microglia, and in a cell-free reconstructed system that pinocytosed proteins become degraded immediately following pinocytosis and the resulting peptides are selectively delivered to major histocompatibility complex class II (MHC-II) containing lysosomes, whereas undegraded proteins are retained in the pinosomal lumen. This early size-based sorting of pinosomal contents relied on the formation of transient tunnel between pinosomes and lysosomes in a Rab7- and dynamin II-dependent manner, which allowed the small contents to pass through but restricted large ones. Inhibition of the size-based sorting markedly reduced proliferation and cytokine release of cocultured CD4 + T cells, indicating that the size-based sorting is required for efficient antigen presentation by microglial cells. Together, these findings reveal a novel early sorting mechanism for pinosomal luminal contents in microglial cells, which may explain how microglia efficiently process protein antigens and evoke an immune response. [ABSTRACT FROM AUTHOR]

Five new sulfide diketopiperazine derivatives, namely, penicibrocazines A-E (1-5), along with a known congener (6), were isolated and identified from the culture extract of Penicillium brocae MA-231, an endophytic fungus obtained from the fresh tissue of the marine mangrove plant Avicennia marina. The structures of these compounds were elucidated by detailed interpretation of NMR and mass spectroscopic data and the structures of compounds 1 and 3 were confirmed by single-crystal X-ray diffraction analysis. All these compounds were examined for cytotoxic and antimicrobial activities. Compounds 2-6 exhibited antimicrobial activity against some of the tested strains with MIC values ranging from 0.25 to 64 μg/mL. [ABSTRACT FROM AUTHOR]