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This study is intended for decision-makers in the field of water resources use (irrigation and hydropower) and for emergency prevention authorities. Another problem remains intensive technogenic pollution of the environment, including water bodies, and one of the main tasks in the conditions of technogenesis is the development of predictive models for the migration of polluting chemical elements and substances in the environment. To calculate the runoff of the river the HBV3-ETH9 hydrological model and the CMIP5 RCP4.5 and RCP8.5 climate projections were used, according to which the air temperature is expected to increase by 2.3 and 4.3 °C, respectively, in annual precipitation - by 10% of the norm. In the period 2023-2080, a gradual increase in annual runoff is expected by 9-23%, and according to the extreme scenario, by 9-36% of the norm, which is associated with intensive glacier water loss, seasonal snowmelt and rainfall. In the future, the passage of two flood peaks (in June and July) is possible. The increase in water content in April and May will contribute to increased mudflow and flood activity.

The electronic educational environment is now being actively implemented in the field of vocational education all over the world. The concept of digital competence and the readiness of a university teacher to use the Moodle distance learning management system are considered. The study involved 45 respondents - teachers of the humanities at a technical university, 23 - with an academic degree, 21 - higher education without an academic degree. The described study based on the analysis of questionnaire answers reveals significant differences in the components of digital competence of teachers of different generations.

Abstract Background The Wnt/β-catenin signalling is aberrantly activated in primary B cell chronic lymphocytic leukaemia (CLL). Epigenetic silencing of pathway inhibitor genes may be a mechanism for its activation. In this study, we investigated systematically and quantitatively the methylation status of 12 Wnt/β-catenin pathway inhibitor genes – CDH1, DACT1, DKK1, DKK2, DKK3, DKK4, SFRP1, SFRP2, SFRP3, SFRP4, SFRP5 and WIF1 – in the cell lines EHEB and MEC-1 as well as patient samples. Methods Quantification of DNA methylation was performed by means of bisulphite pyrosequencing and confirmed by bisulphite Sanger sequencing. Gene expression was analysed by qPCR using GAPDH as internal control. E-cadherin and β-catenin protein quantification was carried out by microsphere-based immunoassays. Methylation differences observed between the patient and control groups were tested using generalised least squares models. Results For 10 genes, a higher methylation level was observed in tumour material. Only DKK4 exhibited similarly high methylation levels in both tumour and normal specimens, while DACT1 was always essentially unmethylated. However, also for these inhibitors, treatment of cells with the demethylating agent 5-aza-2´-deoxycytidine resulted in an induction of their expression, as shown by quantitative PCR, suggesting an indirect epigenetic control of activity. While the degree of demethylation and its transcriptional consequences differed between the genes, there was an overall high correlation of demethylation and increased activity. Protein expression studies revealed that no constitutive Wnt/β-catenin signalling occurred in the cell lines, which is in discrepancy with results from primary CLL. However, treatment with 5-aza-2´-deoxycytidine caused accumulation of β-catenin. Simultaneously, E-cadherin expression was strongly induced, leading to the formation of a complex with β-catenin and thus demonstrating its epigenetically regulated inhibition effect. Conclusions The results suggest an epigenetic silencing mechanism of the Wnt/β-catenin pathway inhibitor genes in CLL. Hypermethylation and silencing of functionally related genes may not be completely stochastic but result from the tumour epigenome reprogramming orchestrated by Polycomb-group repressive complexes. The data are of interest in the context of epigenetic-based therapy.