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9 results on '"Chow, Louise T."'

1. Human papillomavirus (HPV) origin-binding protein associates with mitotic spindles to enable viral DNA partitioning

Author

Van Tine, Brian A., Dao, Luan D., Wu, Shwu-Yuan, Sonbuchner, Timothy M., Lin, Biing Yuan, Zou, Nianxiang, Chiang, Cheng-Ming, Broker, Thomas R., and Chow, Louise T.

Subjects
Papillomaviruses -- Research, Science and technology
Abstract

Human papillomaviruses (HPVs) establish long-term infections in patients. The mechanism for extrachromosomal HPV DNA persistence in cycling cells is unknown. We show that HPV origin-containing plasmids partition as minichromosomes, attributable to an association of the viral origin recognition protein E2 with mitotic spindles. [alpha]-, [beta]-, and [gamma]-tubulins were pulled down with a tagged E2. The N-terminal transacting and C-terminal protein dimerization/DNA binding domains independently associated with the spindles. We suggest that this E2 property enables these viruses to establish persistence. Its implication for HPV oncogenesis is presented.

Published
2004

2. Cell cycle-regulated phosphorylation of p220(super.NPAT) by cyclin E/Cdk2 in Cajal bodies promotes histone gene transcription

Author

Ma, Tianlin, Van Tine, Brian A., Wei, Yue, Garrett, Michelle D., Nelson, David, Adams, Peter D., Wang, Jin, Qin, Jun, Chow, Louise T., and Harper, J. Wade

Subjects
Cytogenetics -- Research, Histones -- Genetic aspects, Genetic transcription -- Physiological aspects, Cell cycle -- Physiological aspects, Phosphorylation -- Physiological aspects, Protein kinases -- Physiological aspects, Biological sciences
Abstract

Cell cycle-regulated phosphorylation of p220(super.NPAT) by cyclin E/Cdk2 in Cajal bodies has been shown to promote histone gene transcription. In investigation of how cyclin E/Cdk2 controls S-phase events, the subcellular localization of the cyclin E/Cdk2 interacting protein p220(super.NPAT) and its regulation by phosphorylation was studied, and it was found that p220 is localized to discrete nuclear foci. Five cyclin E/Cdk2 phosphorylation sites in p220 were found.

Published
2000

3. Interaction between cyclin-dependent kinases and human papillomavirus replication-initiation protein E1 is required for efficient viral replication

Author

Ma, Tianlin, Zou, Nianxiang, Lin, Biing Yuan, Chow, Louise T., and Harper, J. Wade

Subjects
Papillomaviruses -- Research, Protein kinases -- Research, Phosphorylation -- Research, Science and technology
Abstract

We have identified the human papillomavirus (HPV) DNA replication initiation protein E1 as a tight-binding substrate of cyclin E/cyclin-dependent kinase (Cdk) complexes by using expression cloning. E1, a DNA helicase, collaborates with the HPV E2 protein in ori-dependent replication. El formed complexes with cyclin E in insect and mammalian cells, independent of Cdks and E2. Additional cyclins, including A-, B-, and F-type (but not D-type), interacted with the E1/E2 complex, and A- and E-type cyclin kinases were capable of phosphorylating E1 and E2 in vitro. Association with cyclins and efficient phosphorylation of E1 required the presence of a cyclin interaction motif (the RXL motif). E1 lacking the RXL motif displayed defects in E2-dependent HPV ori replication in vivo. Consistent with a role for Cdk-mediated phosphorylation in E1 function, an E1 protein lacking all four candidate Cdk phosphorylation sites still associated with E2 and cyclin E but was impaired in HPV replication in vitro and in vivo. Our data reveal a link between cyclin/Cdk function and activation of HPV DNA replication through targeting of Cdk complexes to the E1 replication-initiation protein and suggest a functional role for E1 phosphorylation by Cdks. The use of cyclin-binding RXL motifs is now emerging as a major mechanism by which cyclins are targeted to key substrates.

Published
1999

4. Viral E1 and E2 proteins support replication of homologous and heterologous papillomaviral origins

Author

Chang, Cheng-Ming, Ustav, Mart, Stenlund, Arne, Ho, Thau F., Broker, Thomas R., and Chow, Louise T.

Subjects
Viruses -- Reproduction, Papillomaviruses -- Growth, DNA, Science and technology
Abstract

A plasmid was constructed to contain the human papillomavirus (HPV) and its upstream regulatory region and was tested for its ability to support extrachromosomal replication. The results showed that the system was able to support autonomous HPV DNA replication in cell culture. Viral replication required the presence of the HPV E1 and E2 proteins. The ori region for many heterologous HPVs showed a generality of recognition toward either set of the E1 or E2 replication proteins.

Published
1992

5. Identification of mutations in the COL4A5 collagen gene in Alport syndrome

Author

Barker, David F., Hostikka, Sirkka Liisa, Zhou, Jing, Chow, Louise T., Oliphant, Arnold R., Gerken, Steven C., Gregory, Martin C., Skolnick, Mark H., Atkin, Curtis L., and Tryggvason, Karl

Subjects
Collagen diseases -- Genetic aspects -- Research, Genetic disorders -- Research -- Genetic aspects, Glomerulonephritis -- Genetic aspects -- Research, Deafness -- Genetic aspects -- Research, Alport's syndrome -- Genetic aspects -- Research, Science and technology, Genetic aspects, Research
Abstract

Identification of Mutations in the COL4A5 Collagen Gene in Alport Syndrome ALPORT SYNDROME (AS) IS A HETEROGENEOUS group of hereditary glomerulonephritides, the majority of which appear to be X-linked. The [...]

Published
1990

6. The differential expression of the HER-2/neu oncogene among high-risk human papillomavirus-infected glandular lesions of the uterine cervix

Author

Roland, Phillip Y., Stoler, Mark H., Broker, Thomas R., and Chow, Louise T.

Subjects
In situ hybridization, Oncogenes -- Physiological aspects, Gene expression -- Measurement, Cervical cancer -- Genetic aspects, Health
Abstract

A genetic testing procedure called in situ hybridization appears to be a preferable way to identify a gene called HER-2/neu frequently found in cervical cancer cells. In situ hybridization and antibody testing were used to identify HER-2/neu in 15 cervical adenocarcinoma tissue samples. Two-thirds of the samples were found to contain HER-2/neu. Eight samples containing HER-2/neu were identified with in situ hybridization testing while five were identified with antibody testing. Significantly more samples infected with human papillomavirus (HPV) 16 contained HER-2/neu than did those infected with HPV 18.

Published
1997

7. The differential expression of the HER-2/neu oncogene among high-risk human papillomavirus-infected glandular lesions of the uterine cervix

Author

Roland, Phillip Y., Stoler, Mark H., Broker, Thomas R., and Chow, Louise T.

Subjects
Papillomavirus infections -- Genetic aspects, Health
Abstract

Byline: Phillip Y. Roland, Mark H. Stoler, Thomas R. Broker, Louise T. Chow Keywords: HER-2/neu oncogene; human papillomavirus; uterine cervix Abstract: OBJECTIVE: Our purpose was to examine the relationship between HER-2/neu expression and human papillomavirus infection in cervical glandular neoplasia. STUDY DESIGN: Cases of cervical adenocarcinoma in situ and invasive adenocarcinoma were selected for study. Human papillomavirus typing was performed by in situ hybridization. HER-2/neu was detected by in situ hybridization and immunohistochemistry. Fisher's exact test was used to assess for statistical significance. RESULTS: Fifteen cases of adenocarcinoma in situ and invasive adenocarcinoma were analyzed. In situ hybridization detected HER-2/neu messenger ribonucleic acid in 8 cases, whereas immunohistochemistry detected protein in 5 cases. Overall, HER-2/neu activity was present in 10 cases (66.7%). HER-2/neu messenger ribonucleic acid was detected more commonly in lesions associated with human papillomavirus type 16 versus type 18 (85.7% vs 25.0%, p = 0.04). CONCLUSION: HER-2/neu is frequently expressed in human papillomavirus-infected glandular lesions of the cervix. In situ hybridization may provide a more sensitive indicator of HER-2/neu activity over immunohistochemistry. Preferential expression of HER-2/neu messenger ribonucleic acid was detected in human papillomavirus type 16 versus type 18 lesions. Further study is warranted to examine relationships between human papillomavirus infection and HER-2/neu expression. (Am J Obstet Gynecol 1997;177:133-8) Author Affiliation: Birmingham, Alabama, and Charlottesville, Virginia Article History: Received 18 September 1996; Revised 1 November 1996; Accepted 11 February 1997 Article Note: (footnote) [star] From the University of Alabama at Birmingham and the University of Virginia Health Sciences Center., [star][star] Supported in part by National Cancer Institute grant No. CA43629 (M.H.S, L.T.C., and T.R.B.)., a Reprint requests: Phillip Y. Roland, MD, Department of Obstetrics and Gynecology, University of Alabama at Birmingham, OHB Suite 538, 618 S. 20th St., Birmingham, AL 35233-7333., aa 6/1/81066

Published
1997

8. The differential expression of the HER-2/neu oncogene among high-risk human papillomavirus-infected glandular lesions of the uterine cervix

Author

Roland, Phillip Y., Stoler, Mark H., Broker, Thomas R., and Chow, Louise T.

Subjects
Papillomavirus infections -- Genetic aspects, Papillomavirus infections -- Analysis, Messenger RNA -- Analysis, Immunohistochemistry -- Analysis, Health
Abstract

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/S0002-9378(97)70451-4 Byline: Phillip Y. Roland, Mark H. Stoler, Thomas R. Broker, Louise T. Chow Keywords: HER-2/neu oncogene; human papillomavirus; uterine cervix Abstract: OBJECTIVE: Our purpose was to examine the relationship between HER-2/neu expression and human papillomavirus infection in cervical glandular neoplasia. STUDY DESIGN: Cases of cervical adenocarcinoma in situ and invasive adenocarcinoma were selected for study. Human papillomavirus typing was performed by in situ hybridization. HER-2/neu was detected by in situ hybridization and immunohistochemistry. Fisher's exact test was used to assess for statistical significance. RESULTS: Fifteen cases of adenocarcinoma in situ and invasive adenocarcinoma were analyzed. In situ hybridization detected HER-2/neu messenger ribonucleic acid in 8 cases, whereas immunohistochemistry detected protein in 5 cases. Overall, HER-2/neu activity was present in 10 cases (66.7%). HER-2/neu messenger ribonucleic acid was detected more commonly in lesions associated with human papillomavirus type 16 versus type 18 (85.7% vs 25.0%, p = 0.04). CONCLUSION: HER-2/neu is frequently expressed in human papillomavirus-infected glandular lesions of the cervix. In situ hybridization may provide a more sensitive indicator of HER-2/neu activity over immunohistochemistry. Preferential expression of HER-2/neu messenger ribonucleic acid was detected in human papillomavirus type 16 versus type 18 lesions. Further study is warranted to examine relationships between human papillomavirus infection and HER-2/neu expression. (Am J Obstet Gynecol 1997;177:133-8) Author Affiliation: Birmingham, Alabama, and Charlottesville, Virginia Article History: Received 18 September 1996; Revised 1 November 1996; Accepted 11 February 1997 Article Note: (footnote) [star] From the University of Alabama at Birmingham and the University of Virginia Health Sciences Center., [star][star] Supported in part by National Cancer Institute grant No. CA43629 (M.H.S, L.T.C., and T.R.B.)., a Reprint requests: Phillip Y. Roland, MD, Department of Obstetrics and Gynecology, University of Alabama at Birmingham, OHB Suite 538, 618 S. 20th St., Birmingham, AL 35233-7333., aa 6/1/81066

Published
1997

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