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1. Highly discriminatory single-nucleotide polymorphism interrogation of Escherichia coli by use of allele-specific real-time PCR and eBURST analysis

Author

Sheludchenko, Maxim S., Huygens, Flavia, and Hargreaves, Megan H.

Subjects
Escherichia coli -- Genetic aspects, Escherichia coli -- Physiological aspects, Microbial source tracking -- Evaluation, Polymerase chain reaction -- Usage, Public health -- Management, Single nucleotide polymorphisms -- Usage, Company business management, Biological sciences
Abstract

A single-nucleotide polymorphism (SNP) genotyping approach was applied to differentiate Escherichia coli isolates from six different animal species in Southeast Queensland (SEQ), Australia. The results confirmed the potential application of SNP typing as a promising method for the discrimination of host-specific groups and allows for the identification of human-specific E. coli in environmental samples.

Published
2010

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