54 results on '"Brignone J"'
Search Results
2. Development and standardization of an enzyme-linked inmunosorbent for the detection of orthohantavirus infection in Argentina based on its bacterial-expressed nucleocapside protein.
- Author
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Muzulin PM, Brignone J, Iglesias NG, Rodríguez M, Irazu L, García JB, Sen C, and Levis S
- Subjects
- Humans, Argentina, Nucleocapsid Proteins immunology, Nucleocapsid Proteins genetics, Recombinant Proteins genetics, Recombinant Proteins immunology, Immunoglobulin G blood, Antigens, Viral, Enzyme-Linked Immunosorbent Assay methods, Orthohantavirus immunology, Orthohantavirus genetics, Orthohantavirus isolation & purification, Hantavirus Infections diagnosis, Antibodies, Viral blood, Sensitivity and Specificity
- Abstract
We conducted a development and standardization of an IgG ELISA assay for serological detection of human orthohantavirus infections using the recombinant antigen rLECH13 produced in bacterial and derived from the LECHV. The evaluation and standardization were carried out by analyzing serum samples from a total of 50 patients with confirmed Hantavirus Pulmonary Syndrome (HPS) diagnosis through the reference technique, 50 negative sera, and 53 patients with other medical conditions. The data from the assay analysis showed a diagnostic sensitivity value of 95% and a diagnostic specificity of 80%. The high sensitivity of this novel assay leads us to conclude that rLECH13 is a feasible option for use in the immunodiagnostic of orthohantavirus infection. Additionally, it is crucial to have an antigen that can be produced under conditions that do not require highly complex laboratories. Furthermore, the new assay is cost-effective, reproducible, and demonstrates excellent performance., (© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
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- 2024
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3. Correction: Development and standardization of an enzyme-linked inmunosorbent for the detection of orthohantavirus infection in Argentina based on its bacterial-expressed nucleocapside protein.
- Author
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Muzulin PM, Brignone J, Iglesias NG, Rodríguez M, Irazu L, García JB, Sen C, and Levis S
- Published
- 2024
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4. Novel Oliveros-like Clade C Mammarenaviruses from Rodents in Argentina, 1990-2020.
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Shedroff E, Martin ML, Whitmer SLM, Brignone J, Garcia JB, Sen C, Nazar Y, Fabbri C, Morales-Betoulle M, Mendez J, Montgomery J, Morales MA, and Klena JD
- Subjects
- Animals, Humans, Rodentia, Argentina epidemiology, Arenaviridae genetics, Hemorrhagic Fever, American epidemiology, Arenaviruses, New World genetics, Junin virus genetics, Arenavirus genetics
- Abstract
Following an Argentine Hemorrhagic Fever (AHF) outbreak in the early 1990s, a rodent survey for Junín virus, a New World Clade B arenavirus, in endemic areas of Argentina was conducted. Since 1990, INEVH has been developing eco-epidemiological surveillance of rodents, inside and outside the Argentine Hemorrhagic Fever endemic area. Samples from rodents captured between 1993 and 2019 that were positive for Arenavirus infection underwent Sanger and unbiased, Illumina-based high-throughput sequencing, which yielded 5 complete and 88 partial Mammarenaviruses genomes. Previously, 11 genomes representing four species of New World arenavirus Clade C existed in public records. This work has generated 13 novel genomes, expanding the New World arenavirus Clade C to 24 total genomes. Additionally, two genomes exhibit sufficient genetic diversity to be considered a new species, as per ICTV guidelines (proposed name Mammarenavirus vellosense ). The 13 novel genomes exhibited reassortment between the small and large segments in New World Mammarenaviruses . This work demonstrates that Clade C Mammarenavirus infections circulate broadly among Necromys species in the Argentine Hemorrhagic Fever endemic area; however, the risk for Clade C Mammarenavirus human infection is currently unknown.
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- 2024
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5. Preliminary Study on Effects of Neck Exoskeleton Structural Design in Patients With Amyotrophic Lateral Sclerosis.
- Author
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Demaree D, Brignone J, Bromberg M, and Zhang H
- Subjects
- Humans, Male, Female, Middle Aged, Biomechanical Phenomena, Aged, Electromyography, Head Movements, Neck physiopathology, Equipment Design, Adult, Muscle Weakness physiopathology, Amyotrophic Lateral Sclerosis physiopathology, Exoskeleton Device, Neck Muscles physiopathology
- Abstract
Neck muscle weakness due to amyotrophic lateral sclerosis (ALS) can result in dropped head syndrome, adversely impacting the quality of life of those affected. Static neck collars are currently prescribed to hold the head in a fixed upright position. However, these braces are uncomfortable and do not allow any voluntary head-neck movements. By contrast, powered neck exoskeletons have the potential to enable head-neck movements. Our group has recently improved the mechanical structure of a state-of-the-art neck exoskeleton through a weighted optimization. To evaluate the effect of the structural changes, we conducted an experiment in which patients with ALS were asked to perform head-neck tracking tasks while using the two versions of the neck exoskeleton. We found that the neck muscle activation was significantly reduced when assisted by the structurally enhanced design compared to no assistance provided. The improved structure also improved kinematics tracking performance, allowing users to better achieve the desired head poses. In comparison, the previous design did not help reduce the muscle effort required to perform these tasks and even slightly worsened the kinematic tracking performance. It was also found that biomechanical benefits gained from using the structurally improved design were consistent across participants with both mild and severe neck weakness. Furthermore, we observed that participants preferred to use the powered neck exoskeletons to voluntarily move their heads and make eye contact during a conversation task rather than remain in a fixed upright position. Each of these findings highlights the importance of the structural design of neck exoskeletons in achieving desired biomechanical benefits and suggests that neck exoskeletons can be a viable method to improve the daily life of patients with ALS.
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- 2024
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6. cis -Acting Element at the 5' Noncoding Region of Tacaribe Virus S RNA Modulates Genome Replication.
- Author
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D'Antuono AL, Gallo GL, Sepulveda C, Fernández J, Brignone J, Gamboa G, Riera L, Saavedra MDC, and López N
- Subjects
- Humans, RNA, Viral genetics, Mutagenesis, Promoter Regions, Genetic genetics, Arenaviruses, New World genetics, Arenaviruses, New World pathogenicity, Genome, Viral, RNA Replication genetics
- Abstract
Tacaribe virus (TCRV) is the prototype of New World mammarenaviruses, a group that includes several members that cause hemorrhagic fevers in humans. The TCRV genome comprises two RNA segments, named S (small) and L (large). Both genomic segments contain noncoding regions (NCRs) at their 5' and 3' ends. While the 5'- and 3'-terminal 19-nucleotide sequences are known to be essential for promoter function, the role of their neighboring internal noncoding region (iNCR) sequences remains poorly understood. To analyze the relevance of the 5' and 3' iNCRs in TCRV S RNA synthesis, mutant S-like minigenomes and miniantigenomes were generated. Using a minireplicon assay, Northern blotting, and reverse transcription-quantitative PCR, we demonstrated that the genomic 5' iNCR is specifically engaged in minigenome replication yet is not directly involved in minigenome transcription, and we showed that the S genome 3' iNCR is barely engaged in this process. Analysis of partial deletions and point mutations, as well as total or partial substitution of the 5' iNCR sequence, led us to conclude that the integrity of the whole genomic 5' iNCR is essential and that a local predicted secondary structure or RNA-RNA interactions between the 5' and 3' iNCRs are not strictly required for viral S RNA synthesis. Furthermore, we employed a TCRV reverse genetic approach to ask whether manipulation of the S genomic 5' iNCR sequence may be suitable for viral attenuation. We found that mutagenesis of the 5' promoter-proximal subregion slightly impacted recombinant TCRV virulence in vivo . IMPORTANCE The Mammarenavirus genus of the Arenaviridae family includes several members that cause severe hemorrhagic fevers associated with high morbidity and mortality rates, for which no FDA-approved vaccines and limited therapeutic resources are available. We provide evidence demonstrating the specific involvement of the TCRV S 5' noncoding sequence adjacent to the viral promoter in replication. In addition, we examined the relevance of this region in the context of an in vivo infection. Our findings provide insight into the mechanism through which this 5' viral RNA noncoding region assists the L polymerase for efficient viral S RNA synthesis. Also, these findings expand our understanding of the effect of genetic manipulation of New World mammarenavirus sequences aimed at the rational design of attenuated recombinant virus vaccine platforms.
- Published
- 2023
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7. Protective effect of sacubitril/valsartan (Entresto) on kidney function and filtration barrier injury in a porcine model of partial nephrectomy.
- Author
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Brignone J, Jensen M, Jensen BL, Assersen KB, Goetze JP, Jødal L, Andersen TB, Magnusdottir SO, Kloster B, Jønler M, and Lund L
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- Animals, Swine, Valsartan, Aminobutyrates, Kidney, Nephrectomy, Drug Combinations, Glomerular Filtration Rate, Tetrazoles, Biphenyl Compounds
- Abstract
Kidney surgery often includes organ ischaemia with a risk of acute kidney injury. The present study tested if treatment with the combined angiotensin II-angiotensin II receptor type 1 and neprilysin blocker Entresto (LCZ696, sacubitril/valsartan) protects filtration barrier and kidney function after ischaemia and partial nephrectomy (PN) in pigs. Single kidney glomerular filtration rate (GFR) by technetium-99m diethylene-triamine-pentaacetate clearance was validated (n = 6). Next, four groups of pigs were followed for 15 days (n = 24) after PN (one-third right kidney, 60 min ischaemia) + Entresto (49/51 mg/day; n = 8), PN + vehicle (n = 8), sham + Entresto (49/51 mg/day; n = 4) and sham + vehicle (n = 4). GFR, diuresis and urinary albumin were measured at baseline and from each kidney after 15 days. The sum of single-kidney GFR (right 25 ± 6 mL/min, left 31 ± 7 mL/min) accounted for the total GFR (56 ± 14 mL/min). Entresto had no effect on baseline blood pressure, p-creatinine, mid-regional pro-atrial natriuretic peptide (MR-proANP), heart rate and diuresis. After 15 days, Entresto increased GFR in the uninjured kidney (+23 ± 6 mL/min, P < .05) and reduced albuminuria from both kidneys. In the sham group, plasma MR-proANP was not altered by Entresto; it increased to similar levels 2 h after surgery with and without Entresto. Fractional sodium excretion increased with Entresto. Kidney histology and kidney injury molecule-1 in cortex tissue were not different. In conclusion, Entresto protects the filtration barrier and increases the functional adaptive response of the uninjured kidney., (© The Author(s) 2022. Published by Oxford University Press on behalf of the ERA.)
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- 2023
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8. First study of Seoul virus (SEOV) in urban rodents from newly urbanized areas of Gran La Plata, Argentina.
- Author
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Fitte B, Brignone J, Sen C, and Robles MDR
- Subjects
- Rats, Animals, Rodentia, Argentina epidemiology, Ecosystem, Zoonoses, Seoul virus genetics
- Abstract
Alterations of ecosystems have deep effects on the distribution of parasites. Big cities of Argentina present structural features that favor the presence of synanthropic species, acting as source of zoonotic diseases, for example in urban rodents: the Norway rat (Rattus norvegicus) and the black rat (R. rattus). One of the important zoonotic pathogens related are the RNA virus Hantavirus, with high prevalence rates in South America. The aim of this study was to explore and identify the presence of Hantavirus in urban rodents from Gran La Plata, Argentina. The presence of anti-hantavirus IgG antibodies was determined by the Enzyme-Linked Immunosorbent Assay. Six samples turned out positive for Seoul virus (SEOV, p = 14.3%). These are the first records of SEOV in urban rodents in Gran La Plata. It represents the first report in R. rattus in Argentina, and in America. This situation underscores the inequality and historical forgetfulness of a portion of society, calling for urgent action to be taken in this regard., Competing Interests: Conflicts of interest The authors reported no potential conflict of interest., (Copyright © 2022. Published by Elsevier España, S.L.U.)
- Published
- 2023
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9. [Robotic surgery used in urology].
- Author
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Jølner M, Brignone J, Fabrin K, Rawashdeh YF, and Lund L
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- Humans, Urologic Surgical Procedures adverse effects, Urologic Surgical Procedures methods, Laparoscopy methods, Robotic Surgical Procedures methods, Robotics, Urology
- Abstract
Robotic surgery has been used in urology for more than two decades. It is a minimally invasive procedure with less morbidity, mortality and length of hospital stay compared to open surgery. The advantage of robotic surgery has led to a shift in the treatment of patients with urological cancers and some benign reconstructive procedures. However, we need RCT's in order to prove the benefit of robotic surgery compared to open procedures due to the treatment of patients but also to secure economic issues for the society, as argued in this review.
- Published
- 2022
10. Chapare Hemorrhagic Fever and Virus Detection in Rodents in Bolivia in 2019.
- Author
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Loayza Mafayle R, Morales-Betoulle ME, Romero C, Cossaboom CM, Whitmer S, Alvarez Aguilera CE, Avila Ardaya C, Cruz Zambrana M, Dávalos Anajia A, Mendoza Loayza N, Montaño AM, Morales Alvis FL, Revollo Guzmán J, Sasías Martínez S, Alarcón De La Vega G, Medina Ramírez A, Molina Gutiérrez JT, Cornejo Pinto AJ, Salas Bacci R, Brignone J, Garcia J, Añez A, Mendez-Rico J, Luz K, Segales A, Torrez Cruz KM, Valdivia-Cayoja A, Amman BR, Choi MJ, Erickson BR, Goldsmith C, Graziano JC, Joyce A, Klena JD, Leach A, Malenfant JH, Nichol ST, Patel K, Sealy T, Shoemaker T, Spiropoulou CF, Todres A, Towner JS, and Montgomery JM
- Subjects
- Animals, Bolivia epidemiology, Cross Infection transmission, Cross Infection virology, Disease Transmission, Infectious, Hemorrhagic Fevers, Viral genetics, Hemorrhagic Fevers, Viral transmission, Hemorrhagic Fevers, Viral virology, High-Throughput Nucleotide Sequencing, Humans, Polymerase Chain Reaction, Rats virology, Viral Zoonoses transmission, Viral Zoonoses virology, Arenaviruses, New World genetics, Arenaviruses, New World isolation & purification, Hemorrhagic Fever, American complications, Hemorrhagic Fever, American genetics, Hemorrhagic Fever, American transmission, Hemorrhagic Fever, American virology, RNA, Viral genetics, RNA, Viral isolation & purification, Rodentia virology
- Abstract
Background: In June 2019, the Bolivian Ministry of Health reported a cluster of cases of hemorrhagic fever that started in the municipality of Caranavi and expanded to La Paz. The cause of these cases was unknown., Methods: We obtained samples for next-generation sequencing and virus isolation. Human and rodent specimens were tested by means of virus-specific real-time quantitative reverse-transcriptase-polymerase-chain-reaction assays, next-generation sequencing, and virus isolation., Results: Nine cases of hemorrhagic fever were identified; four of the patients with this illness died. The etiologic agent was identified as Mammarenavirus Chapare mammarenavirus , or Chapare virus (CHAPV), which causes Chapare hemorrhagic fever (CHHF). Probable nosocomial transmission among health care workers was identified. Some patients with CHHF had neurologic manifestations, and those who survived had a prolonged recovery period. CHAPV RNA was detected in a variety of human body fluids (including blood; urine; nasopharyngeal, oropharyngeal, and bronchoalveolar-lavage fluid; conjunctiva; and semen) and in specimens obtained from captured small-eared pygmy rice rats ( Oligoryzomys microtis ). In survivors of CHHF, viral RNA was detected up to 170 days after symptom onset; CHAPV was isolated from a semen sample obtained 86 days after symptom onset., Conclusions: M. Chapare mammarenavirus was identified as the etiologic agent of CHHF. Both spillover from a zoonotic reservoir and possible person-to-person transmission were identified. This virus was detected in a rodent species, O. microtis . (Funded by the Bolivian Ministry of Health and others.)., (Copyright © 2022 Massachusetts Medical Society.)
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- 2022
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11. Protection of kidney function and tissue integrity by pharmacologic use of natriuretic peptides and neprilysin inhibitors.
- Author
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Brignone J, Assersen KB, Jensen M, Jensen BL, Kloster B, Jønler M, and Lund L
- Subjects
- Angiotensin Receptor Antagonists pharmacology, Angiotensin Receptor Antagonists therapeutic use, Animals, Humans, Kidney drug effects, Kidney physiology, Natriuretic Peptides therapeutic use, Acute Kidney Injury drug therapy, Kidney metabolism, Natriuretic Peptides pharmacology, Neprilysin antagonists & inhibitors
- Abstract
With variable potencies atrial-, brain-type and c-type natriuretic peptides (NP)s, best documented for ANP and its analogues, promote sodium and water excretion, renal blood flow, lipolysis, lower blood pressure, and suppress renin and aldosterone secretion through interaction predominantly with cGMP-coupled NPR-A receptor. Infusion of especially ANP and its analogues up to 50 ng/kg/min in patients with high risk of acute kidney injury (cardiac vascular bypass surgery, intraabdominal surgery, direct kidney surgery) protects kidney function (GFR, plasma flow, medullary flow, albuminuria, renal replacement therapy, tissue injury) at short term and also long term and likely additively with the diuretic furosemide. This documents a pharmacologic potential for the pathway. Neprilysin (NEP, neutral endopeptidase) degrades NPs, in particular ANP, and angiotensin II. The drug LCZ696, a mixture of the neprilysin inhibitor sacubitril and the ANGII-AT1 receptor blocker valsartan, was FDA approved in 2015 and marketed as Entresto®. In preclinical studies of kidney injury, LCZ696 and NPs lowered plasma creatinine, countered hypoxia and oxidative stress, suppressed proinflammatory cytokines, and inhibited fibrosis. Few randomized clinical studies exist and were designed with primary cardiac outcomes. The studies showed that LCZ696/entresto stabilized and improved glomerular filtration rate in patients with chronic kidney disease. LCZ696 is safe to use concerning kidney function and stabilizes or increases GFR. In perspective, combined AT1 and neprilysin inhibition is a promising approach for long-term renal protection in addition to AT1 receptor blockers in acute kidney injury and chronic kidney disease.
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- 2021
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12. Orthohantavirus genotype Lechiguanas in Oligoryzomys nigripes (Rodentia: Cricetidae): New evidence of host-switching.
- Author
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Colombo VC, Brignone J, Sen C, Previtali MA, Martin ML, Levis S, Monje L, González-Ittig R, and Beldomenico PM
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- Animals, Genotype, Humans, Arvicolinae genetics, Arvicolinae virology, Host Microbial Interactions genetics, RNA Viruses genetics
- Abstract
To identify and predict situations of increased risk of orthohantavirus infection in humans, it is necessary to study the relationships between the virus and its rodent hosts. The present study investigated orthohantavirus infection in an assemblage of wild Sigmodontinae rodents of the Paraná Delta, Argentina, and providing new evidence of host-switching events. Rodents belonging to the species Oxymycterus rufus (n = 187), Akodon azarae (n = 82), Oligoryzomys flavescens (n = 80), Oligoryzomys nigripes (n = 47), Scapteromys aquaticus (n = 38), Deltamys kempi (n = 7) and Holochilus brasiliensis (n = 2) were captured at 4 sampling sites during 20 trapping sessions. Blood samples were analyzed by IgG ELISA and livers by a nested reverse transcription PCR for the diagnosis of orthohantavirus infection. The amplified products of the S and M orthohantavirus genomes were sequenced and analyzed to determine similarities with species of the Orthohantavirus genus. The species of the Oligoryzomys positive to the virus were confirmed by amplifying and sequencing the complete cyt b gene. Of the 443 serum samples analyzed by IgG ELISA, A. azarae presented the highest host-specific prevalence value (10/82, 12.2%) followed by Ol. nigripes (4/47, 8.5%) and Ox. rufus (1/187, 0.5%). All the sero-positive Ol. nigripes (n = 4) were positive to the amplification of the S and M segments of the Lechiguanas genotype (98% nucleotide identity for both segments). This is surprising given that Ol. nigripes has been previously associated with Juquitiba genotype, not Lechiguanas. The latter is generally associated with Ol. flavescens, which in our study were all sero-negative. In addition, the association Ox. rufus - Pergamino genotype found here is, to our knowledge, novel and another potential evidence of host-switching considering that Pergamino has been originally associated with A. azarae. These findings contribute to the building evidence that contradicts the one-genotype-one-reservoir species premise in the association between rodent reservoirs and orthohantaviruses, and supports the hypothesis that the community structure of sympatric host species may contribute to orthohantavirus dynamics., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2019
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13. Assessing cross-reactivity of Junín virus-directed neutralizing antibodies.
- Author
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Leske A, Waßmann I, Schnepel K, Shifflett K, Holzerland J, Bostedt L, Bohn P, Mettenleiter TC, Briggiler AM, Brignone J, Enria D, Cordo SM, Hoenen T, and Groseth A
- Subjects
- Arenaviruses, New World immunology, HEK293 Cells, Hemorrhagic Fever, American immunology, Humans, Virus Replication, Antibodies, Neutralizing immunology, Antibodies, Viral immunology, Cross Reactions, Junin virus immunology
- Abstract
Arenaviruses cause several viral hemorrhagic fevers endemic to Africa and South America. The respective causative agents are classified as biosafety level (BSL) 4 pathogens. Unlike for most other BSL4 agents, for the New World arenavirus Junín virus (JUNV) both a highly effective vaccination (Candid#1) and a post-exposure treatment, based on convalescent plasma transfer, are available. In particular, neutralizing antibodies (nAbs) represent a key protective determinant in JUNV infection, which is supported by the correlation between successful passive antibody therapy and the levels of nAbs administered. Unfortunately, comparable resources for the management of other closely related arenavirus infections are not available. Given the significant challenges inherent in studying BSL4 pathogens, our goal was to first assess the suitability of a JUNV transcription and replication-competent virus-like particle (trVLP) system for measuring virus neutralization under BSL1/2 conditions. Indeed, we could show that infection with JUNV trVLPs is glycoprotein (GP) dependent, that trVLP input has a direct correlation to reporter readout, and that these trVLPs can be neutralized by human serum with kinetics similar to those obtained using authentic virus. These properties make trVLPs suitable for use as a proxy for virus in neutralization assays. Using this platform we then evaluated the potential of JUNV nAbs to cross-neutralize entry mediated by GPs from other arenaviruses using JUNV (strain Romero)-based trVLPs bearing GPs either from other JUNV strains, other closely related New World arenaviruses (e.g. Tacaribe, Machupo, Sabiá), or the distantly related Lassa virus. While nAbs against the JUNV vaccine strain are also active against a range of other JUNV strains, they appear to have little or no capacity to neutralize other arenavirus species, suggesting that therapy with whole plasma directed against another species is unlikely to be successful and that the targeted development of cross-specific monoclonal antibody-based resources is likely needed. Such efforts will be supported by the availability of this BSL1/2 screening platform which provides a rapid and easy means to characterize the potency and reactivity of anti-arenavirus neutralizing antibodies against a range of arenavirus species., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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14. [Outbreak of hantavirus pulmonary syndrome in Tucumán, Argentina].
- Author
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Calderón GE, Brignone J, Martin ML, Calleri F, Sen C, Casas N, Calli R, Sinchi A, Enria D, and Levis S
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- Animals, Argentina epidemiology, Child, Preschool, Disease Outbreaks, Disease Reservoirs classification, Enzyme-Linked Immunosorbent Assay, Female, Genotype, Hantavirus Pulmonary Syndrome diagnosis, Humans, Male, Real-Time Polymerase Chain Reaction, Retrospective Studies, Rodentia classification, Young Adult, Antibodies, Viral blood, Disease Reservoirs virology, Orthohantavirus genetics, Orthohantavirus immunology, Hantavirus Pulmonary Syndrome epidemiology, Rodentia virology
- Abstract
We describe an outbreak of hantavirus pulmonary syndrome in the Burruyacú Department, Province of Tucumán. The detection in 2016 of a case of hantavirosis affecting a 23-year-old woman, considered at that time to be the first case occurred in that province, promoted a thorough epidemiological study. The investigation allowed the retrospective detection of another case occurred one month earlier in a 5-year-old child in the same Department. In both cases, the infection was confirmed by serology (case 1 at days 4 and 7 of disease onset, case 2 at day 4) and the viral genotype was characterized as HU39694. The contacts of both cases were serologically negative for hantavirus. The rodents captured in the area belonged to genus Akodon, genus Calomys and species Mus musculus. Oligoryzomys, the known reservoir for this viral genotype, was not found. Specific anti-hantavirus antibodies were not detected in the captured rodents. Given that the patients had not visited hantavirus endemic areas and their contacts were negative for hantavirus, we infer that the patients were locally exposed to fluids of infected rodents during their usual social or recreational outdoor activities. In conclusion, we demonstrate that hantavirus HU39694 -a genotype until now considered to be restricted to the Central Pampas of the country- is circulating in the North Western province of Tucumán. The endemic area of hantavirosis is thus expanded to this province but the viral reservoir in the area has not yet been identified.
- Published
- 2018
15. [Autochthonous case of spotted fever caused by Rickettsia parkeri in Ensenada, Buenos Aires].
- Author
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Villalba Apestegui P, Nava S, Brignone J, Sen C, Esposto A, and Angeletti V
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- Adult, Animals, DNA, Bacterial genetics, Dogs, Humans, Male, Polymerase Chain Reaction, Rickettsia classification, Spotted Fever Group Rickettsiosis transmission, Rickettsia genetics, Spotted Fever Group Rickettsiosis diagnosis, Ticks microbiology
- Abstract
We present a case of spotted fever occurred in an adult residing in Ensenada, Buenos Aires province in February 2016. The patient presented with an acute febrile syndrome associated with a skin necrotic lesion on the left leg secondary to a tick bite. The general symptoms were a maculopapular rash, headache, myalgia, and arthralgias. Seroconversion of anti-Rickettsia specific IgG antibodies confirmed recent infection. The nucleotidic and aminoacidic sequences of a gltA gen fragment matched 100% the sequences of R. parkeri strains from Argentina and other countries of America. The patient responded well to treatment with doxycycline.
- Published
- 2018
16. Cadmium chloride prevents the rise in rat brown adipose tissue mitochondrial respiration in response to acute cold stress.
- Author
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Noli MI, Pavia MA Jr, Mignone IR, Brignone JA, Hagmüller K, and Zaninovich AA
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- Adipose Tissue, Brown physiology, Animals, Body Temperature Regulation drug effects, Male, Mitochondria metabolism, Oxygen Consumption, Rats, Rats, Wistar, Triiodothyronine administration & dosage, Adipose Tissue, Brown drug effects, Cadmium Chloride toxicity, Cold Temperature, Mitochondria drug effects
- Published
- 1998
- Full Text
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17. Favourable, significant effect of the dose-dependent treatment with RU 38486 (RU) on the alterations of the hepatic mitochondrial function of diabetic rats.
- Author
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Brignone JA, de Brignone CM, Ricci CR, de Mignone IR, Susemihl MC, and Rodríguez RR
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- 3-Hydroxybutyric Acid, Adrenalectomy, Animals, Anti-Inflammatory Agents pharmacology, Corticosterone pharmacology, Diabetes Mellitus, Experimental enzymology, Dose-Response Relationship, Drug, Electron Transport Complex IV drug effects, Electron Transport Complex IV metabolism, Female, Hydroxybutyrate Dehydrogenase drug effects, Hydroxybutyrate Dehydrogenase metabolism, Hydroxybutyrates metabolism, Malates metabolism, Mitochondria, Liver enzymology, Mitochondria, Liver metabolism, Oxygen Consumption physiology, Rats, Succinates metabolism, Succinic Acid, Diabetes Mellitus, Experimental metabolism, Hormone Antagonists pharmacology, Mifepristone pharmacology, Mitochondria, Liver drug effects, Oxygen Consumption drug effects
- Abstract
In the present work, the effect "in vivo' of increasing doses of RU 38486 upon the hepatic mitochondrial function of diabetic rats has been studied. At the same time, the action of adrenalectomy and corticosterone restitution on this function were comparatively demonstrated. The parameters measured were oxygen consumption with the substrates: 3-hydroxybutyrate (HB), succinate (Suc) and malate-glutamate (Mal-glut) in intact liver mitochondria and the activities of 3-hydroxybutyrate dehydrogenase (HBD) and cytochrome c oxidase (Cyt.c oxid.) enzymes in broken liver mitochondria. The groups of animals studied were normal controls (N) and the following groups of diabetic rats: rats without any treatment (D), adrenalectomized rats (D+ADX), rats that were adrenalectomized and treated with corticosterone (D+ADX+C) and four groups treated with increasing oral doses of RU (in mg/kg body wt.), that is, 12.5 (D+RU1), 25.0 (D+RU2), 37.5 (D+RU3) and 50.0 (D+RU4). The results showed a tendency of increasing values of mitochondrial oxygen consumption in diabetic animals treated with RU. The favourable effect of increasing doses of RU on O2 consumption of diabetic rat liver mitochondria with each of the substrates showed a significant association as indicated by the values obtained for the correlation coefficients r (0.95, 0.97 and 0.99 according to the substrate HB, Succ or Mal-glut, respectively). Likewise, the correlation between the treatment with increasing doses of RU and the recovery of enzyme activities showed a significant dose-effect association with r 0.94 for HBD and r = 0.95 for Cyt.c oxid. Adrenalectomy showed a similar effect to treatment with the maximum dose of RU while corticosterone restitution gave measured values similar to those of the D group. In conclusion, the favourable, significant variation of the hepatic mitochondrial function of diabetic rats was demonstrated by the dose-dependent treatment with RU as seen by the correlation statistical study performed. At the same time, the pernicious effect that glucocorticoids exert upon such function in experimental diabetes was confirmed.
- Published
- 1996
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18. Preparation of pig pure islets without using density gradients.
- Author
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Abalovich A, Mignone I, Susemihl C, Ricci C, Migliore S, Rodriguez R, Brignone C, and Brignone J
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- Animals, Centrifugation methods, Centrifugation, Density Gradient, Collagenases, Evaluation Studies as Topic, In Vitro Techniques, Islets of Langerhans Transplantation, Swine, Cell Separation methods, Islets of Langerhans cytology
- Published
- 1995
19. Effects of thyroid hormones on mitochondrial oxygen consumption in brown adipose tissue and heart from cold-exposed hypothyroid rats.
- Author
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Cageao LF, Mignone IR, Ricci CR, Brignone CC, Brignone JA, and Zaninovich AA
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- Adipose Tissue ultrastructure, Adipose Tissue, Brown ultrastructure, Animals, Body Temperature Regulation drug effects, Body Temperature Regulation physiology, Glycerolphosphate Dehydrogenase metabolism, Hypothyroidism pathology, Hypothyroidism physiopathology, Iopanoic Acid pharmacology, Male, Mitochondria drug effects, Mitochondria physiology, Mitochondria, Heart drug effects, Mitochondria, Heart physiology, Myocardium ultrastructure, Organ Size, Oxygen Consumption physiology, Radioimmunoassay, Rats, Rats, Inbred Strains, Thyroxine metabolism, Triiodothyronine metabolism, Adipose Tissue metabolism, Adipose Tissue, Brown metabolism, Cold Temperature, Hypothyroidism metabolism, Mitochondria metabolism, Mitochondria, Heart metabolism, Myocardium metabolism, Oxygen Consumption drug effects, Thyroxine pharmacology, Triiodothyronine pharmacology
- Abstract
The present work measured brown adipose tissue and heart mitochondrial oxygen consumption in hypothyroid rats treated with replacement doses of T3, T4 or T4 plus iopanoic acid and kept at 4 degrees C for 24 h. Heart oxygen consumption in normal, untreated hypothyroid and T4-treated hypothyroid rats was unaffected by cold exposure. In rats treated with T4 plus iopanoic acid, rates of oxygen consumption were normal in those maintained at 4 degrees C as well as in those kept at room temperature, despite serum T3 concentration being significantly decreased. The cold-exposed T3-treated hypothyroid rats showed a marked decrease in oxygen consumption (p less than 0.02) and alpha-glycerophosphate dehydrogenase activity, a T3-dependent enzyme. Mitochondrial oxygen consumption in brown fat from normal (p less than 0.01), T4 (p less than 0.02) and T4 plus iopanoic acid-treated (p less than 0.01) rats rose more than twofold in response to cold. In the T3-treated group, oxygen consumption at room temperature was higher (p less than 0.02) than in any other group at similar temperatures. However, the T3-treated group showed no changes in oxygen consumption in response to cold, perhaps because this group reached the maximal response at room temperature. The untreated and the T3-treated hypothyroid rats (both groups devoid of T4) did not survive at 4 degrees C unless T4 or several-fold replacement amounts of T3 were administered. The data demonstrate the crucial role of T4 in thermogenesis during cold exposure.
- Published
- 1992
- Full Text
- View/download PDF
20. Improving effects obtained by the ovariectomy or treatment with tamoxifen of female diabetic rats over the function and enzyme activities of liver mitochondria.
- Author
-
Brignone JA, de Brignone CM, de Mignone IR, Ricci CR, Susemihl MC, and Rodríguez RR
- Subjects
- Analysis of Variance, Animals, Diabetes Mellitus, Experimental enzymology, Electron Transport Complex IV metabolism, Estradiol physiology, Female, Hydroxybutyrate Dehydrogenase metabolism, Mitochondria, Liver drug effects, Oxygen Consumption, Rats, Diabetes Mellitus, Experimental physiopathology, Mitochondria, Liver enzymology, Mitochondria, Liver physiology, Ovariectomy, Tamoxifen pharmacology
- Abstract
In the present work we studied, in female chronic diabetic rats the effect of either the parenteral administration of tamoxifen (TAM) (500 micrograms.kg-1.day-1) for 15 days or the ovariectomy upon the respiration and oscillatory behaviour of intact mitochondria and the activities of 3-hydroxybutyrate dehydrogenase (HBD) and cytochrome c oxidase (Cox) of disrupted liver mitochondria. The treatment with TAM as well as the ovariectomy of diabetic animals significantly increased the respiratory control (RC) and the state 3 (S3) of respiration of intact liver mitochondria with the three substrates assayed (3-hydroxybutyrate, malate-glutamate and succinate). Both treatments also lowered significantly the damped factors of the oscillatory variation of liver intact mitochondria of diabetic rats. Moreover, the two above-mentioned treatments restored the activities of HBD and Cox of liver disrupted mitochondria to normal values. The effect of estrogens at level of its receptors in the modulation of liver mitochondrial function and liver HBD and Cox activities in chronic diabetes is discussed.
- Published
- 1991
- Full Text
- View/download PDF
21. Effects of withdrawal of glucocorticoids on improving the function and enzymatic activities of liver mitochondria in female diabetic rats.
- Author
-
Brignone JA, Campos de Brignone CM, Rebagliati de Mignone IR, Ricci CR, Susemihl MC, and Rodríguez RR
- Subjects
- Adrenalectomy, Animals, Corticosterone administration & dosage, Diabetes Mellitus, Experimental, Female, Mitochondria, Liver enzymology, Oxygen Consumption drug effects, Rats, Corticosterone pharmacology, Electron Transport Complex IV metabolism, Hydroxybutyrate Dehydrogenase metabolism, Mitochondria, Liver physiology, Succinate Dehydrogenase metabolism
- Abstract
In the present work the effects of corticosterone restitution were examined in female rats with chronic streptozotocin (SZ)-induced diabetes upon intact liver mitochondrial function and the activities of 3-hydroxybutyrate dehydrogenase (HBD), succinate dehydrogenase (SD) and cytochrome c oxidase (Cox) of the ruptured organelle. The liver mitochondrial function was analyzed by the respiration and the osmotic oscillatory behaviour. Respiration was measured by polarographic method and both the state 3 of active respiration (S3) and the respiratory control (RC) were determined using the following substrates: 3-hydroxybutyrate, succinate and malate-glutamate. The oscillatory behaviour was measured using as parameters the damping factors (DF) which are the ratios of amplitudes of two consecutive peaks or troughs of the spectrophotometrical tracings of this phenomenon. A group of control normal rats (N) and the following three groups of diabetic rats were studied: controls (D), adrenalectomized (D + ADX) and adrenalectomized with corticosterone restitution (D + ADX + C). The results of mitochondrial respiration showed that the mean values of S3 and RC decreased with the three substrates in the group D + ADX + C compared with D + ADX group (p < 0.001). This group demonstrated a significant increase of S3 and RC values of the respiration compared with the D group. The oscillatory behaviour of liver mitochondria of D + ADX + C group demonstrated a significant increase in the DF of peaks and troughs compared with D + ADX group. The values of DF of the latter group were not significantly different from the N group. The behaviour of the enzymes activities of ruptured liver mitochondria were different for each enzyme in the different groups of treated rats. Thus, in the D + ADX + C group the mean value of the activity of HBD significantly decreased, that of the Cox increased (p < 0.02) and that of SD did not show any variation compared with the corresponding values of the D + ADX group. Likewise, the mean value of HBD activity in this latter group was similar to that of the N group and that of Cox activity was lesser (p < 0.01) than that of the D group. The conclusion is drawn that corticosterone has significant additional diabetogenic effects upon biochemical functions of liver mitochondria in the SZ-induced diabetic state which could occur through the hormone cellular receptors.
- Published
- 1991
22. [Effects of cold on myocardial mitochondria respiration of rats with hypothyroidism treated with triiodothyronine].
- Author
-
Cageao LF, de Mignone IR, Ricci CR, de Brignone CM, Altschuler LR, Brignone JA, and Zaninovich AA
- Subjects
- Adaptation, Physiological, Animals, Body Temperature Regulation, Hypothyroidism drug therapy, Hypothyroidism physiopathology, Male, Rats, Rats, Inbred Strains, Thyroid Hormones therapeutic use, Triiodothyronine administration & dosage, Cold Temperature, Glycerolphosphate Dehydrogenase metabolism, Mitochondria, Heart physiology, Oxygen Consumption physiology
- Abstract
The present work studied the effect of cold on oxygen consumption (OC) and alpha-glycerophosphate dehydrogenase activity (alpha-GPD) in heart mitochondria of hypothyroid rats (hypo) treated with T3, T4 or T4 plus Iopanoic Acid (IOP). 200 g male Wistar rats were made hypothyroid by 131I administration. Animals were injected s.c., in divided doses, for 10 days, with one of the following substances: T3, 300 ng/100 g BW/day; T4, 2 micrograms/100 g BW/day or T4 plus IOP, 5 mg/100 g BW/day, for 72 h preceding the experiment. One half of each group was housed in a cold room at 4 degrees C and the other at 22 degrees C, for 25 h, and thereafter decapitated. Heart mitochondria were isolated by routine methods. The OC was measured polarographically using L-malate, L-glutamate and malonate as substrates. Intramitochondrial alpha-GPD activity was measured by a microcolorimetric assay. The results from 16 or 20 rats/group (4 or 5 pools of 4 hearts each) were: In the rats kept at 22 degrees C the OC (in ng at. oxyg./min/mg prot.; State 3) in the hypo+T4 group was 69 +/- 10; in the rats treated with T4+IOP, 75 +/- 11 and in the hypo+T3, 102 +/- 5. When the animals were exposed to 4 degrees C no change was observed in the hypo+T4 and hypo+T4IOP groups. On the other hand, OC was significantly lower in the T3-treated animals (p less than 0.001, versus their controls at 22 degrees C). This group of rats did not survive when exposed to cold.(ABSTRACT TRUNCATED AT 250 WORDS)
- Published
- 1991
23. [Changes in liver mitochondria in rat after treatment with oral contraceptives].
- Author
-
Brignone JA, de Brignone CM, Gamboni M, Koch O, and Stoppani AO
- Subjects
- Animals, Castration, Female, Hydroxybutyrate Dehydrogenase pharmacology, Male, Mitochondria, Liver drug effects, Mitochondria, Liver ultrastructure, Rats, Diethylstilbestrol pharmacology, Mestranol pharmacology, Mitochondria, Liver metabolism, Norethindrone pharmacology
- Published
- 1978
24. Effect in vivo of endogenous sexual hormones upon the liver mitochondrial function compared in male and female diabetic rats.
- Author
-
Brignone JA, Campos de Brignone CM, Rodríguez RR, Marzi A, Rebagliati de Mignone I, Rodoni MJ, and Susemihl MC
- Subjects
- Animals, Female, Hydroxybutyrate Dehydrogenase metabolism, Male, Mitochondria, Liver metabolism, Mitochondria, Liver physiology, Rats, Streptozocin, Diabetes Mellitus, Experimental physiopathology, Estradiol pharmacology, Mitochondria, Liver drug effects, Orchiectomy, Ovariectomy, Oxygen Consumption drug effects
- Abstract
Chronic diabetes induced by the injection of streptozotocin in male and female albino adult rats provoked significant alteration of liver mitochondrial function 30 or 35 days after administration of the drug. Thus, we obtained mean values of respiratory control (RC) and state 3 (S3) with 3-hydroxybutyrate as substrate 40 or 50% lower than those of non-diabetic animals. With other substrates (malate-glutamate, succinate) the decrease of RC and S3 in the diabetic animals was 20% or 30% of the normal mean values. The osmotic damped oscillations of mitochondria were measured as another parameter of the organella function. It was assayed with valinomycin as K+ ionophore and succinate as substrate. In diabetic rats of both sexes we found a significant increase of the mean damping factor of these oscillatory variations compared with normal values. The above-mentioned results indicate a lesser elasticity and an impaired K+ transport of mitochondria across the inner membrane in diabetic animals. Both reported parameters, respiration and oscillatory variations of liver mitochondria, were measured in normal non-diabetic rats and in the groups of diabetic rats referred to as follows: 1) intact (male and female), 2) gonadectomized (male and female), 3) oophorectomized with restitution of 17 beta-estradiol. Ovariectomized diabetic rats showed a significant increase in the values of the RC and S3 of liver mitochondria compared with intact female diabetic animals. The withdrawal of the ovarian hormone in female diabetic rats significantly decreased the values of the damping factors of the oscillatory mechanism and they were similar to the normal. The restitution of 17 beta-estradiol to oophorectomized diabetic rats resulted in a decrease of liver mitochondrial respiration. The damping factor of liver mitochondria of the oophorectomized diabetic rats treated with the estrogen showed values significantly higher than those of female diabetic animals without the hormone and similar to the values of the intact diabetic female rats. Castration of male rats did not produce any effect upon the liver mitochondrial RC and S3 or upon the mean damping factor of the oscillatory variation either. Then the castration of male diabetic rats did not modify the mitochondrial function. In contrast, the oophorectomy of diabetic animals produced amelioration of mitochondrial respiration and oscillatory behavior. The conclusion is drawn that in female rats the circulating 17 beta-estradiol produced a pernicious effect upon liver mitochondrial function in the experimental diabetic state.
- Published
- 1989
25. Modulation of D-3-hydroxybutyrate dehydrogenase activity by estrogens.
- Author
-
Brignone JA, Ricci CR, de Brignone CM, Labonia NA, and Stoppani AO
- Subjects
- Age Factors, Animals, Estradiol pharmacology, Female, Kidney enzymology, Male, Mestranol pharmacology, Mitochondria, Heart enzymology, Mitochondria, Liver enzymology, Ovariectomy, Rats, Sex Factors, Estrogens pharmacology, Hydroxybutyrate Dehydrogenase antagonists & inhibitors
- Abstract
Liver D-3-hydroxybutyrate dehydrogenase (OHBD) is subjected to estrogen modulation. Estrogen action was demonstrated by (a) the lesser activity of liver OHBD in female rats, as compared with their male counterparts; (b) the increase of OHBD activity after ovariectomy of sexually mature rats; (c) the decrease of OHBD activity after treatment of gonadectomized or normal rats with 17 beta-estradiol or with artificial estrogens; (d) the decrease of OHBD activity in female rats during sexual development; (e) the effects of tamoxifen on the enzyme activity. The kinetics of OHBD reaction using liver mitochondria from estrogen-treated rats showed a 50% decrease of Vmax, as compared with the control value, in contrast to the other parameters which did not vary. These results, taken together with the effect of estrogens on liver mitochondrial phospholipids, point to a decreased content of OHBD in liver mitochondria from estrogen-treated rats. In contrast to OHBD, succinate dehydrogenase and cytochrome oxidase activities, mitochondrial protein synthesis and L-malate + L-glutamate oxidation by coupled liver mitochondria either increased or were not affected by estrogens. Kidney and heart OHBD were affected by ovariectomy and estrogens like the liver enzyme, though to a lesser degree.
- Published
- 1987
26. [Hepatotoxic effect of 2 anovulatory steroids, mestranol and norethisterone acetate in the rat].
- Author
-
Brignone JA, De Brignone CM, De Dávila MT, Koch O, and Stoppani AO
- Subjects
- Adenosine Triphosphatases metabolism, Animals, Calcium physiology, Female, Hydroxybutyrate Dehydrogenase antagonists & inhibitors, Male, Microscopy, Electron, Mitochondria, Liver metabolism, Mitochondria, Liver ultrastructure, Phospholipids metabolism, Rats, Mestranol adverse effects, Mitochondria, Liver drug effects, Norethindrone adverse effects
- Published
- 1979
27. [Liver mitochondria changes in chronic diabetes induced by streptozotocin or pancreatectomy].
- Author
-
Brignone JA, Campos de Brignone CM, Badano BN, Rodríguez RR, and Stoppani AO
- Subjects
- Adenosine Triphosphatases metabolism, Animals, Insulin metabolism, Male, Pancreatectomy, Rats, Streptozocin, Diabetes Mellitus, Experimental enzymology, Hydroxybutyrate Dehydrogenase metabolism, Mitochondria, Liver enzymology
- Published
- 1981
28. Effect of ovarian hormones upon liver mitochondrial function in diabetic rats.
- Author
-
Brignone JA, de Brignone CM, Rodríguez RR, Marzi AA, de Mignone IR, and Susemihl MC
- Subjects
- Animals, Female, Kinetics, Mitochondria, Liver drug effects, Oxygen Consumption drug effects, Rats, Reference Values, Diabetes Mellitus, Experimental metabolism, Estradiol pharmacology, Mitochondria, Liver metabolism, Ovariectomy
- Abstract
In the present study it is shown that streptozotocin (SZ)-induced chronic diabetes of female albino rats produced significant alterations in liver mitochondrial function after 30-35 days of diabetes. The disturbances were as follows: (1) a significant fall of the mean values of the respiratory control ratio and of state 3 of respiration using three substrates, 3-hydroxybutyrate, malate-glutamate and succinate, and (2) a significant increase of the mean damping factor of the oscillatory osmotic variations (with valinomycin as K+ ionophore and succinate as substrate). The same mitochondrial function parameters were analyzed for comparison in control non-diabetic rats (group N) and in the following groups of female rats with chronic diabetes: intact (group I), oophorectomized (6 days after the injection of SZ) (group O), and oophorectomized with restitution therapy of 17 beta-estradiol (from the operation until the day before killing) (group O + Eol). The O group showed significantly higher values of the respiratory control ratio and of state 3 of respiration and significantly lower damping factors than group I. The restitution treatment in the O + Eol group restored the mitochondrial functions assayed to values similar to those of group I. These data provide strong evidence that estrogens exert a negative effect at the molecular level upon impaired liver mitochondrial functions in SZ-induced diabetes.
- Published
- 1988
- Full Text
- View/download PDF
29. Modified oscillation behavior and decreased D-3-hydroxybutyrate dehydrogenase activity in diabetic rat liver mitochondria.
- Author
-
Brignone JA, Campos de Brignone CM, Rodriguez RR, Badano BN, and Stoppani AO
- Subjects
- Animals, Diabetes Mellitus, Experimental drug therapy, Electron Transport Complex IV metabolism, Insulin therapeutic use, Kinetics, Male, Oxygen Consumption, Pancreatectomy, Rats, Spectrophotometry, Diabetes Mellitus, Experimental enzymology, Hydroxybutyrate Dehydrogenase metabolism, Mitochondria, Liver enzymology, Mitochondrial Swelling
- Published
- 1982
- Full Text
- View/download PDF
30. Inhibition of succinic dehydrogenase by polysulfonated compounds.
- Author
-
STOPPANI AO and BRIGNONE JA
- Subjects
- Naphthalenes analogs & derivatives, Succinate Dehydrogenase antagonists & inhibitors, Sulfhydryl Compounds pharmacology, Sulfonic Acids pharmacology, Suramin pharmacology
- Published
- 1957
- Full Text
- View/download PDF
31. [Changes in the metabolism of mitochondrial phospholipids induced by synthetic estrogens].
- Author
-
Brignone JA, De Brignone CM, and Stoppani AO
- Subjects
- Animals, Male, Phosphorus Isotopes, Prostate metabolism, Rats, Diethylstilbestrol pharmacology, Ethers pharmacology, Hexestrol pharmacology, Microsomes metabolism, Mitochondria, Liver metabolism, Phenols pharmacology, Phosphatidylcholines metabolism, Phosphatidylethanolamines metabolism, RNA metabolism
- Published
- 1968
32. [Action of androgens on respiratory systems of myocardium].
- Author
-
De Brignone CM, Stoppani AO, and Brignone JA
- Subjects
- Animals, Swine, Androgens pharmacology, Myocardium enzymology, Myocardium metabolism, Oxidoreductases analysis, Succinate Dehydrogenase analysis
- Published
- 1966
33. [Action of stilbestrol and analogues on the NADH 2-oxidase system of the rat prostate].
- Author
-
Brignone JA, De Brignone CM, and Stoppani AO
- Subjects
- Animals, Male, Rats, Diethylstilbestrol pharmacology, Hexestrol pharmacology, Oxidoreductases analysis, Prostate drug effects, Prostate enzymology
- Published
- 1966
34. [Action of steroid hormones on the NADH-2-oxidase and succinic oxidase systems of animal tissues].
- Author
-
STOPPANI AO, BRIGNONE JA, and DE BRIGNONE CC
- Subjects
- Animals, Humans, Desoxycorticosterone pharmacology, Diethylstilbestrol pharmacology, Estrogens pharmacology, Hormones, Multienzyme Complexes, NAD, NADH, NADPH Oxidoreductases, Oxidoreductases metabolism, Progesterone pharmacology, Steroids, Succinate Dehydrogenase, Testosterone pharmacology
- Published
- 1961
35. Protection of succinic dehydrogenase thiol groups by fluoride and phosphate.
- Author
-
BRIGNONE JA and STOPPANI AO
- Subjects
- Electron Transport Complex II, Fluorides pharmacology, Oxidoreductases, Phosphates pharmacology, Succinate Dehydrogenase, Sulfhydryl Compounds
- Published
- 1956
- Full Text
- View/download PDF
36. [Action of gestagens and corticoids on the respiratory systems of the myocardium].
- Author
-
De Brignone CC, Stoppani AO, and Brignone JA
- Subjects
- Animals, Myocardium metabolism, Swine, Adrenal Cortex Hormones pharmacology, Myocardium enzymology, Oxidoreductases metabolism, Progestins pharmacology, Succinate Dehydrogenase metabolism
- Published
- 1967
37. Factors affecting succinoxidase sensitivity toward oxidation with BAL.
- Author
-
BRIGNONE JA and STOPPANI AO
- Subjects
- Dimercaprol metabolism, Oxidation-Reduction, Oxidoreductases
- Published
- 1957
- Full Text
- View/download PDF
38. [Effect of diethylstilbestrol on the metabolism of liver mitochondrial and microsomal phospholipids].
- Author
-
Brignone JA, De Brignone CM, and Stoppani AO
- Subjects
- Animals, Diethylstilbestrol administration & dosage, Injections, Subcutaneous, Male, Phosphatidylcholines metabolism, Phosphatidylethanolamines metabolism, Phosphorus Isotopes, Rats, Time Factors, Diethylstilbestrol pharmacology, Microsomes, Liver metabolism, Mitochondria, Liver metabolism, Phospholipids metabolism
- Published
- 1970
39. [Protection of succinic dehydrogenase thiols by phosphate ions].
- Author
-
STOPPANI AO and BRIGNONE JA
- Subjects
- Electron Transport Complex II, Ions, Oxidoreductases, Phosphates, Phosphoric Monoester Hydrolases pharmacology, Succinate Dehydrogenase, Sulfhydryl Compounds
- Published
- 1955
40. Inhibition by steroid hormones of quinone reduction by heart-muscle preparations.
- Author
-
STOPPANI AO, BRIGNONE JA, DE BRIGNONE CC, and BADANO BN
- Subjects
- Aged, Humans, Benzoquinones, Hormones pharmacology, Myocardium metabolism, Quinones metabolism, Steroids
- Published
- 1962
- Full Text
- View/download PDF
41. [Effect of sulfonated urea compounds on myocardial succinoxidase].
- Author
-
STOPPANI AO and BRIGNONE JA
- Subjects
- Myocardium metabolism, Oxidoreductases, Urea
- Published
- 1955
42. [Action of estrogens on the NADH2-oxidase systems of the myocardium and kidney].
- Author
-
De Brignone CM, Stoppani AO, and Brignone JA
- Subjects
- Animals, Male, Rats, Estrogens pharmacology, Kidney enzymology, Myocardium enzymology, Oxidoreductases metabolism
- Published
- 1967
43. [Action of estrogens on myocardial and kidney NADH2-oxidase systems].
- Author
-
de Brignone CM, Stoppani AO, and Brignone JA
- Subjects
- Animals, Dogs, Electron Transport, NAD, Estrogens pharmacology, Kidney enzymology, Myocardium enzymology, Oxidoreductases metabolism
- Published
- 1969
44. [In vivo action of synthetic estrogens on the NADH2-oxidase system in rat organs].
- Author
-
Brignone JA, de Brignone CM, and Stoppani AO
- Subjects
- Adrenalectomy, Animals, Castration, Heart Ventricles drug effects, Kidney drug effects, Liver drug effects, Rats, Diethylstilbestrol pharmacology, Hexestrol pharmacology, Kidney enzymology, Liver enzymology, Muscles enzymology, Myocardium enzymology, Oxidoreductases metabolism
- Published
- 1968
45. [Action in vivo of artificial estrogens on the NADH 2-oxidase system of rat organs].
- Author
-
Brignone JA, De Brignone CM, and Stoppani AO
- Subjects
- Animals, Male, Rats, Estrogens pharmacology, Kidney enzymology, Liver enzymology, Myocardium enzymology, Oxidoreductases analysis
- Published
- 1966
46. [Effect of diethylstilbestrol on phospholipid metabolism in liver mitochondria and microsomes].
- Author
-
Brignone JA, de Brignone CM, and Stoppani AO
- Subjects
- Animals, Male, Rats, Diethylstilbestrol pharmacology, Microsomes, Liver metabolism, Mitochondria, Liver metabolism, Phospholipids metabolism
- Published
- 1971
47. [Action of androgens on the electron transport systems in the myocardium].
- Author
-
de Brignone CM, Stoppani AO, and Brignone JA
- Subjects
- Androstenols pharmacology, Androsterone pharmacology, Animals, Desoxycorticosterone pharmacology, In Vitro Techniques, Methandrostenolone pharmacology, Methyltestosterone pharmacology, Oxidoreductases antagonists & inhibitors, Swine, Testosterone pharmacology, Androgens pharmacology, Electron Transport drug effects, Myocardium enzymology
- Published
- 1968
48. Structural requirements for the action of steroids as inhibitors of electron transfer.
- Author
-
Stoppani AO, De Brignone CM, and Brignone JA
- Subjects
- Androgens pharmacology, Androstanes pharmacology, Animals, Chemical Phenomena, Chemistry, Depression, Chemical, Glucocorticoids pharmacology, NAD, Norsteroids pharmacology, Oxidoreductases antagonists & inhibitors, Spectrophotometry, Swine, Adrenal Cortex Hormones pharmacology, Electron Transport drug effects, Gonadal Steroid Hormones pharmacology, Mitochondria, Muscle enzymology, Myocardium enzymology, Steroids pharmacology, Succinate Dehydrogenase antagonists & inhibitors
- Published
- 1968
- Full Text
- View/download PDF
49. [Protective effects of polysulfonated compounds on succinic dehydrogenase thiols].
- Author
-
STOPPANI AO and BRIGNONE JA
- Subjects
- Electron Transport Complex II, Oxidoreductases, Succinate Dehydrogenase, Sulfhydryl Compounds, Suramin pharmacology
- Published
- 1955
50. [Protective effects of phosphate ions of thiol groups on succinic dehydrogenases].
- Author
-
STOPPANI AO and BRIGNONE JA
- Subjects
- Electron Transport Complex II, Oxidoreductases, Phosphates pharmacology, Succinate Dehydrogenase, Sulfhydryl Compounds
- Published
- 1955
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