1. Long-distance turgor pressure changes induce local activation of plant glutamate receptor-like channels.
- Author
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Grenzi M, Buratti S, Parmagnani AS, Abdel Aziz I, Bernacka-Wojcik I, Resentini F, Šimura J, Doccula FG, Alfieri A, Luoni L, Ljung K, Bonza MC, Stavrinidou E, and Costa A
- Subjects
- Receptors, Glutamate genetics, Receptors, Glutamate metabolism, Glutamic Acid, Pressure, Plant Leaves metabolism, Gene Expression Regulation, Plant, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism, Arabidopsis metabolism
- Abstract
In Arabidopsis thaliana, local wounding and herbivore feeding provoke leaf-to-leaf propagating Ca
2+ waves that are dependent on the activity of members of the glutamate receptor-like channels (GLRs). In systemic tissues, GLRs are needed to sustain the synthesis of jasmonic acid (JA) with the subsequent activation of JA-dependent signaling response required for the plant acclimation to the perceived stress. Even though the role of GLRs is well established, the mechanism through which they are activated remains unclear. Here, we report that in vivo, the amino-acid-dependent activation of the AtGLR3.3 channel and systemic responses require a functional ligand-binding domain. By combining imaging and genetics, we show that leaf mechanical injury, such as wounds and burns, as well as hypo-osmotic stress in root cells, induces the systemic apoplastic increase of L-glutamate (L-Glu), which is largely independent of AtGLR3.3 that is instead required for systemic cytosolic Ca2+ elevation. Moreover, by using a bioelectronic approach, we show that the local release of minute concentrations of L-Glu in the leaf lamina fails to induce any long-distance Ca2+ waves., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2023 Elsevier Inc. All rights reserved.)- Published
- 2023
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