28 results on '"Nagata, Tetsu"'
Search Results
2. Indomethacin enhances learning and memory potential by interacting with CaMKII.
- Author
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Kanno T, Yaguchi T, Nagata T, Shimizu T, Tanaka A, and Nishizaki T
- Subjects
- Adult, Animals, Anti-Inflammatory Agents, Non-Steroidal administration & dosage, Anti-Inflammatory Agents, Non-Steroidal metabolism, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Cells, Cultured, Excitatory Postsynaptic Potentials drug effects, Excitatory Postsynaptic Potentials physiology, Female, Hippocampus cytology, Hippocampus drug effects, Hippocampus enzymology, Hippocampus pathology, Humans, Indomethacin pharmacology, Male, Maze Learning physiology, Memory physiology, Mice, Middle Aged, Neurons drug effects, Neurons enzymology, Neurons pathology, Organ Culture Techniques, Rats, Rats, Wistar, Xenopus, Young Adult, Calcium-Calmodulin-Dependent Protein Kinase Type 2 metabolism, Indomethacin administration & dosage, Indomethacin metabolism, Maze Learning drug effects, Memory drug effects
- Abstract
The present study examined the effect of indomethacin (IM), a cyclooxygenase inhibitor, on learning and memory functions. IM activated Ca(2+) /calmodulin-dependent protein kinase II (CaMKII) in cultured rat hippocampal neurons. IM (100 µM) significantly increased the rate of spontaneous AMPA receptor-mediated miniature excitatory postsynaptic currents elicited from CA1 pyramidal neurons of rat hippocampal slices, without affecting the amplitude, and enhanced extracellular high K(+) (20 mM)-induced glutamate release from rat hippocampal slices, indicating that IM stimulates presynaptic glutamate release. Those IM effects were clearly inhibited by the CaMKII inhibitor KN-93. IM persistently facilitated synaptic transmission monitored from the CA1 region of rat hippocampal slices in a concentration (1-100 µM)-dependent manner that was also abolished by KN-93. In the water maze test, IM (1 mg/kg, i.p.) enhanced spatial learning and memory ability for normal rats, and ameliorated scopolamine-induced spatial learning and memory impairment or age-related spatial learning and memory deterioration for senescence-accelerated mouse-prone 8 mice. In the test to learn 15 numbers consisting of three patterns of five digit number for healthy human subjects, oral intake with IM (25 mg/kg) significantly raised the scores of correct number arrangements that subjects memorized 5 min and 3 days after the test. The results of the present study indicate that IM could enhance learning and memory potential by facilitating hippocampal synaptic transmission as a result from stimulating presynaptic glutamate release under the control of CaMKII., (Copyright © 2011 Wiley Periodicals, Inc.)
- Published
- 2012
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3. Indomethacin activates protein kinase C and potentiates α7 ACh receptor responses.
- Author
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Kanno T, Yaguchi T, Nagata T, and Nishizaki T
- Subjects
- Animals, Bungarotoxins pharmacology, Excitatory Postsynaptic Potentials drug effects, Hippocampus drug effects, Hippocampus enzymology, Hippocampus metabolism, Indoles pharmacology, Maleimides pharmacology, Nicotinic Antagonists pharmacology, Oocytes drug effects, Oocytes metabolism, Patch-Clamp Techniques, Protein Kinase C antagonists & inhibitors, Rats, Rats, Wistar, Receptors, Nicotinic chemistry, Xenopus growth & development, Xenopus metabolism, alpha7 Nicotinic Acetylcholine Receptor, Indomethacin pharmacology, Protein Kinase C metabolism, Receptors, Nicotinic metabolism
- Abstract
Background/aims: We have earlier found that indomethacin activates CaMKII, as a novel action distinct from COX inhibition. To explore further indomethacin actions, the present study focused upon PKC and examined the effect of indomethacin on α7 ACh receptor responses and hippocampal synaptic transmission through PKC., Methods: We recorded currents through α7 ACh receptors expressed in Xenopus oocytes, quantified PKC activity in the in situ and cell-free PKC assay, and monitored field excitatory postsynaptic potentials (fEPSPs) and miniature excitatory postsynaptic currents (mEPSCs) from the CA1 region of rat hippocampal slices., Results: Indomethacin potentiated α7 ACh receptor currents in a bell-shaped concentration (100 nM-1 mM)-dependent manner, and the potentiating effect was inhibited by the PKC inhibitor GF109203X. Indomethacin activated PKC in a concentration (1-100 μM)-dependent manner for cultured rat hippocampal neurons. Additionally, indomethacin (100 μM) significantly activated PKC-ε under the cell-free conditions. Indomethacin (100 μM) induced a transient huge increase in the fEPSP slope followed by persistent increase, and the former effect was attenuated by the α7 ACh receptor antagonist α-bungarotoxin or GF109203X. Indomethacin (100 μM) also increased the rate of nicotine-evoked mEPSCs, and the effect was prevented by α-bungarotoxin or GF109203X., Conclusion: The results of the present study show that indomethacin activates PKC, possibly PKC-e in the brain, thereby potentiating α7 ACh receptor responses to stimulate presynaptic glutamate release, which in part contributes to facilitation of hippocampal transmission. This extends our knowledge about diverse indomethacin actions., (Copyright © 2012 S. Karger AG, Basel.)
- Published
- 2012
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4. DL- and PO-phosphatidylcholines as a promising learning and memory enhancer.
- Author
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Nagata T, Yaguchi T, and Nishizaki T
- Subjects
- Aged, Aged, 80 and over, Animals, Cognition Disorders prevention & control, Disease Models, Animal, Drug Combinations, Female, Humans, Male, Maze Learning drug effects, Memory Disorders prevention & control, Middle Aged, Nootropic Agents pharmacology, Phosphatidylcholines pharmacology, Rats, Rats, Wistar, Scopolamine, Cognition Disorders drug therapy, Memory Disorders drug therapy, Nootropic Agents therapeutic use, Phosphatidylcholines therapeutic use
- Abstract
In the water maze test, oral administration with 1,2-dilynoleoyl-sn-glycero-3-phosphocholine (DLPhtCho)(5 mg/kg) alone or DLPhtCho (5 mg/kg) plus 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPhtCho)(5 mg/kg) significantly shortened the prolonged acquisition latency for rats intraperitoneally injected with scopolamine, with more efficient effect than (POPhtCho)(5 mg/kg) alone, arachidonic acid (AA)(5 mg/kg) alone, docosahexaenoic acid (DHA)(5 mg/kg) alone, or 1-palmitoyl-2-linoleil-sn-glycero-3-phosphoserine (PLPhtSer)(5 mg/kg) alone. POPhtCho (5 mg/kg) alone or DLPhtCho (5 mg/kg) plus POPhtCho (5 mg/kg) also significantly shortened the prolonged retention latency for rats intraperitoneally injected with scopolamine, but otherwise no significant effect was obtained with DLPhtCho (5 mg/kg) alone, AA (5 mg/kg) alone, DHA (5 mg/kg) alone, or PLPhtSer (5 mg/kg) alone. Oral co-administration with DLPhtCho (5 mg/kg) and POPhtCho (5 mg/kg) significantly shortened the acquisition latency for rats untreated with scopolamine as compared with the latency for administration with polyethylene glycol (PEG), DLPhtCho alone at doses of 5 and 10 mg/kg, or POPhtCho alone at doses of 5 and 10 mg/kg, while no efficient effect on the retention latency was obtained. To assess the effect of DLPhtCho and POPhtCho on cognitive functions for humans, Mini Mental State Examination (MMSE) test was performed in subjects with cognitive disorders (the average MMSE score, 15). Oral co-intake with DLPhtCho (50 mg) and POPhtCho (45 mg) once after breakfast everyday raised the score to over 20, corresponding to normal cognitive functions, throughout 5 months after intake, and the increase in the score was significantly greater than that for oral intake with DLPhtCho (100 mg/day) alone or POPhtCho (90 mg/kg) alone. Taken together, the results of the present study show that co-intake with DLPhtCho and POPhtCho could enhance learning and memory ability and improve cognitive disorders for both the animals and humans with a promising efficacy.
- Published
- 2011
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5. AMID mediates adenosine-induced caspase-independent HuH-7 cell apoptosis.
- Author
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Yang D, Yaguchi T, Nagata T, Gotoh A, Dovat S, Song C, and Nishizaki T
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- Apoptosis Regulatory Proteins analysis, Apoptosis Regulatory Proteins genetics, Cell Line, Tumor, Humans, Mitochondrial Proteins analysis, Mitochondrial Proteins genetics, RNA Interference, RNA, Small Interfering metabolism, Up-Regulation, Adenosine metabolism, Apoptosis, Apoptosis Regulatory Proteins metabolism, Caspases metabolism, Mitochondrial Proteins metabolism
- Abstract
Background/aims: The mechanism underlying extracellular adenosine-induced caspase-independent apoptosis in HuH-7 human hepatoma cells is not fully understood. The present study investigated the role for apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death (AMID) in the pathway., Methods: To see the implication of AMID in adenosine-induced HuH-7 cell apoptosis, real-time reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescent cytochemistry, time-laps GFP monitoring, cell cycle analysis, flow cytometry, Western blotting, cell viability assay, and TUNEL staining were carried out., Results: Adenosine upregulated AMID expression in HuH-7 cells, and translocated AMID from the cytosol into the nucleus. Adenosine induced HuH-7 cell apoptosis, and the effect was further enhanced by overexpressing AMID. Adenosine-induced HuH-7 cell apoptosis, alternatively, was inhibited by knocking-down AMID., Conclusion: The results of the present study provide evidence for AMID as a critical factor for adenosine-induced caspase-independent HuH-7 cell apoptosis., (Copyright © 2011 S. Karger AG, Basel.)
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- 2011
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6. E22Δ Mutation in Amyloid β-Protein Promotes β-Sheet Transformation, Radical Production, and Synaptotoxicity, But Not Neurotoxicity.
- Author
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Suzuki T, Murakami K, Izuo N, Kume T, Akaike A, Nagata T, Nishizaki T, Tomiyama T, Takuma H, Mori H, and Irie K
- Abstract
Oligomers of 40- or 42-mer amyloid β-protein (Aβ40, Aβ42) cause cognitive decline and synaptic dysfunction in Alzheimer's disease. We proposed the importance of a turn at Glu22 and Asp23 of Aβ42 to induce its neurotoxicity through the formation of radicals. Recently, a novel deletion mutant at Glu22 (E22Δ) of Aβ42 was reported to accelerate oligomerization and synaptotoxicity. To investigate this mechanism, the effects of the E22Δ mutation in Aβ42 and Aβ40 on the transformation of β-sheets, radical production, and neurotoxicity were examined. Both mutants promoted β-sheet transformation and the formation of radicals, while their neurotoxicity was negative. In contrast, E22P-Aβ42 with a turn at Glu22 and Asp23 exhibited potent neurotoxicity along with the ability to form radicals and potent synaptotoxicity. These data suggest that conformational change in E22Δ-Aβ is similar to that in E22P-Aβ42 but not the same, since E22Δ-Aβ42 exhibited no cytotoxicity, unlike E22P-Aβ42 and wild-type Aβ42.
- Published
- 2010
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7. 1,2-dilinoleoyl-sn-glycero-3-phosphoethanolamine ameliorates age-related spatial memory deterioration by preventing neuronal cell death.
- Author
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Yaguchi T, Nagata T, and Nishizaki T
- Subjects
- Age Factors, Amyloid beta-Peptides pharmacology, Animals, Disease Models, Animal, Drug Administration Schedule, Hippocampus pathology, Male, Mice, Mice, Inbred Strains, PC12 Cells, Peptide Fragments pharmacology, Phosphatidylethanolamines administration & dosage, Rats, Thapsigargin pharmacology, Cell Death drug effects, Cell Survival drug effects, Hippocampus drug effects, Memory Disorders pathology, Memory Disorders prevention & control, Neurons pathology, Phosphatidylethanolamines pharmacology
- Abstract
Background: Accumulating evidence has pointed that a variety of lipids could exert their beneficial actions against dementia including Alzheimer disease and age-related cognitive decline via diverse signaling pathways. Endoplasmic reticulum (ER) stress-induced neuronal apoptosis, on the other hand, is a critical factor for pathogenesis of neurodegenerative diseases such as Alzheimer disease and Parkinson disease, senile dementia, and ischemic neuronal damage. The present study examined the effects of 1,2-dilinoleoyl-sn-glycero-3-phosphoethanolamine (DLPhtEtn), a phospholipid, on ER stress-induced neuronal death and age-related cognitive disorders., Methods: PC-12 cell viability was assayed before and after treatment with amyloid-β(1-40) peptide or thapsigargin in the presence and absence of DLPhtEtn. A series of behavioral tests were performed for senescence-accelerated mouse-prone 8 (SAMP8) mice after 7-month oral administration with polyethylene glycol (PEG) or DLPhtEtn and then, the number of hippocampal neurons was counted., Results: Amyloid-β(1-40) peptide or thapsigargin is capable of causing ER stress-induced apoptosis. DLPhtEtn (30 μM) significantly inhibited PC-12 cell death induced by amyloid-β(1-40) peptide or thapsigargin. In the water maze test, oral administration with DLPhtEtn (1 mg/kg) for 7 months (three times a week) significantly shortened the prolonged retention latency for SAMP8 mice. In contrast, DLPhtEtn had no effect on the acquisition and retention latencies in both the open field test and the passive avoidance test for SAMP8 mice. Oral administration with DLPhtEtn (1 mg/kg) for 7 months prevented a decrease in the number of hippocampal neurons for SAMP8 mice., Conclusion: The results of the present study show that DLPhtEtn ameliorates age-related spatial memory decline without affecting motor activities or fear memory, possibly by protecting hippocampal neuronal death. DLPhtEtn, thus, might exert its beneficial action against senile dementia and neurodegenerative diseases such as Alzheimer disease.
- Published
- 2010
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8. Regulation of AMPA receptor trafficking by O-glycosylation.
- Author
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Kanno T, Yaguchi T, Nagata T, Mukasa T, and Nishizaki T
- Subjects
- Animals, Glycosylation drug effects, Hippocampus drug effects, Hippocampus physiology, Long-Term Potentiation drug effects, Oocytes, Rats, Xenopus, Alloxan pharmacology, N-Acetylglucosaminyltransferases metabolism, Receptors, AMPA metabolism, Synaptic Transmission drug effects
- Abstract
The present study investigated the role of O-linked beta-N-acetylglucosamine (O-GlcNAc) glycosylation (O-GlcNAcylation) in AMPA receptor trafficking. Alloxan, an inhibitor of O-GlcNAc transferase, potentiated responses of AMPA receptors composed of the GluR1 subunit expressed in Xenopus oocytes. No potentiating effect of alloxan was obtained with mutant GluR1 (S831A) receptor lacking CaMKII phosphorylation site. Alloxan facilitated basal synaptic transmission to approximately 120% of basal levels and enhanced Schaffer collateral-CA1 long-term potentiation (LTP) in rat hippocampal slices, especially in the late phase of the LTP. Alloxan stimulated translocation of the GluR1 and GluR2 subunit from the cytosol towards the plasma membrane in rat hippocampal slices with the LTP, although it had no effect on subcellular distribution of the NR1 subunit. Taken together, the results of the present study show that alloxan regulates AMPA receptor trafficking by inhibiting O-GlcNAcylation, to modulate hippocampal synaptic transmission and synaptic plasticity.
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- 2010
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9. DCP-LA neutralizes mutant amyloid beta peptide-induced impairment of long-term potentiation and spatial learning.
- Author
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Nagata T, Tomiyama T, Mori H, Yaguchi T, and Nishizaki T
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- Analysis of Variance, Animals, CA1 Region, Hippocampal physiopathology, Electric Stimulation, Electrophysiology, Excitatory Postsynaptic Potentials drug effects, Excitatory Postsynaptic Potentials physiology, Injections, Intraventricular, Long-Term Potentiation physiology, Male, Maze Learning physiology, Rats, Rats, Wistar, Spatial Behavior drug effects, Spatial Behavior physiology, Amyloid beta-Peptides administration & dosage, CA1 Region, Hippocampal drug effects, Caprylates pharmacology, Long-Term Potentiation drug effects, Maze Learning drug effects
- Abstract
Long-term potentiation (LTP) was monitored from the CA1 region of the intact rat hippocampus by delivering high frequency stimulation (HFS) to the Schaffer collateral commissural pathway. Intraventricular injection with mutant amyloid beta(1-42) peptide lacking glutamate-22 (Abeta(1-42)E22Delta), favoring oligomerization, 10 min prior to HFS, inhibited expression of LTP, with the potency more than wild-type amyloid beta(1-42) peptide. Intraperitoneal injection with the linoleic acid derivative 8-[2-(2-pentyl-cyclopropylmethyl)-cyclopropyl]-octanoic acid (DCP-LA) 70 min prior to HFS neutralized mutant Abeta(1-42)E22Delta peptide-induced LTP inhibition. In the water maze test, continuous intraventricular injection with mutant Abeta(1-42)E22Delta peptide for 14 days prolonged the acquisition latency as compared with that for control, with the potency similar to wild-type Abeta(1-42) peptide, and intraperitoneal injection with DCP-LA shortened the prolonged latency to control levels. The results of the present study indicate that DCP-LA neutralizes mutant Abeta(1-42)E22Delta peptide-induced impairment of LTP and spatial learning.
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- 2010
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10. Interleukin-18 regulates motor activity, anxiety and spatial learning without affecting synaptic plasticity.
- Author
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Yaguchi T, Nagata T, Yang D, and Nishizaki T
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- Animals, Avoidance Learning physiology, Brain physiology, CA1 Region, Hippocampal physiology, Excitatory Postsynaptic Potentials physiology, Exploratory Behavior physiology, Injections, Intraventricular, Interleukin-18 administration & dosage, Interleukin-18 genetics, Lipopolysaccharides, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Spatial Behavior, Synaptic Transmission physiology, Anxiety physiopathology, Interleukin-18 physiology, Maze Learning physiology, Motor Activity physiology, Neuronal Plasticity physiology, Synapses physiology
- Abstract
Expression of Schaffer collateral-CA1 long-term potentiation (LTP) and long-term depression (LTD) was not affected in hippocampal slices from wild-type mice pretreated with lipopolysaccharide (0.25mg/kg, i.p.), to increase interleukin-18 (IL-18) concentrations in the brain. For IL-18 knock-out (IL-18 KO) mice, the LTP was still expressed, the extent being similar to that for wild-type mice. In the open-field test to assess motor activity, rearing activity for IL-18 KO mice was significantly suppressed as compared with that for wild-type mice, without significant difference in the locomotion activity between two groups. In the passive avoidance test to assess fear memory, the retention latency for IL-18 KO mice was much shorter than for wild-type mice, without significant difference in the acquisition latency between two groups. In the water maze test, the acquisition latency for IL-18 KO mice significantly prolonged as compared with that for wild-type mice, without significant difference in the retention latency between two groups. For IL-18 KO mice, intraventricular injection with IL-18 for 4 days (total, 240 fg) prior to water maze task shortened the prolonged acquisition latency, reaching a level similar to that for wild-type mice. The results of the present study, thus, suggest that IL-18 is a critical regulator for exploratory activity, fear memory, and spatial learning.
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- 2010
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11. 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine improves cognitive decline by enhancing long-term depression.
- Author
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Yaguchi T, Nagata T, and Nishizaki T
- Subjects
- Aged, Aged, 80 and over, Animals, Central Nervous System Agents administration & dosage, Cognition Disorders chemically induced, Excitatory Postsynaptic Potentials drug effects, Female, Hippocampus metabolism, Humans, In Vitro Techniques, Male, Maze Learning drug effects, Middle Aged, Phosphatidylcholines administration & dosage, Psychiatric Status Rating Scales, Rats, Rats, Wistar, Receptors, AMPA metabolism, Central Nervous System Agents pharmacology, Cognition Disorders drug therapy, Dementia drug therapy, Hippocampus drug effects, Long-Term Synaptic Depression drug effects, Phosphatidylcholines pharmacology
- Abstract
1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPhtCho) (1 microM) enhanced long-term depression (LTD), a synaptic plasticity relevant to learning and memory, in the CA1 region of rat hippocampal slices, where expression of the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor subunit GluR1 on the plasma membrane was decreased. In the water maze test, oral administration with POPhtCho (5 mg/kg) significantly shortened the prolonged retention latency for rats intraperitoneally injected with scopolamine (1 mg/kg), while the acquisition latency was not affected. For humans with mild cognitive impairment and dementia (average of Mini Mental State Examination score, 18), oral intake with POPhtCho (300 mg/day, once after breakfast) everyday raised the score to over 20, corresponding to normal cognitive functions, throughout 6 months after intake. The results of the present study, thus, indicate that POPhtCho could ameliorate cognitive disorders, possibly by enhancing LTD.
- Published
- 2009
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12. DCP-LA stimulates AMPA receptor exocytosis through CaMKII activation due to PP-1 inhibition.
- Author
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Kanno T, Yaguchi T, Nagata T, Tanaka A, and Nishizaki T
- Subjects
- Animals, Enzyme Activation drug effects, Hippocampus drug effects, Hippocampus enzymology, Male, Rats, Rats, Wistar, Synaptic Transmission drug effects, Calcium-Calmodulin-Dependent Protein Kinase Type 2 metabolism, Caprylates pharmacology, Exocytosis drug effects, Protein Phosphatase 1 antagonists & inhibitors, Receptors, AMPA metabolism
- Abstract
The linoleic acid derivative 8-[2-(2-pentyl-cyclopropylmethyl)-cyclopropyl]-octanoic acid (DCP-LA) activated Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) by inhibiting protein phosphatase-1 (PP-1). DCP-LA induced a transient huge facilitation of synaptic transmission monitored from the CA1 region of rat hippocampal slices, which was largely inhibited by the CaMKII inhibitor KN-93. DCP-LA potentiated kainate-evoked whole-cell membrane currents for Xenopus oocytes expressing alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors composed of the GluR1, GluR3, GluR1/GluR2, GluR1/GluR3, and GluR1/GluR2/GluR3 subunits, and the potentiation was significantly inhibited by KN-93. A similar potentiation was still found with mutant GluR1 (S831A) receptor lacking CaMKII phosphorylation site. The GluR1 and GluR2 subunits formed AMPA receptors in the rat hippocampus, and DCP-LA increased expression of both the subunits on the plasma membrane. The DCP-LA action was blocked by KN-93 and the exocytosis inhibitor botulinum toxin type A, but not by the endocytosis inhibitor phenylarsine oxide. DCP-LA, thus, appears to activate CaMKII through PP-1 inhibition, that stimulates AMPA receptor exocytosis to increase expression of the receptors on the plasma membrane, responsible for potentiate AMPA receptor responses and facilitation of hippocampal synaptic transmission., (Copyright 2009 Wiley-Liss, Inc.)
- Published
- 2009
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13. Dilinoleoylphosphatidylcholine ameliorates scopolamine-induced impairment of spatial learning and memory by targeting alpha7 nicotinic ACh receptors.
- Author
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Yaguchi T, Nagata T, and Nishizaki T
- Subjects
- Animals, Male, Oocytes cytology, Oocytes physiology, Patch-Clamp Techniques, Rats, Rats, Wistar, Spatial Behavior drug effects, Xenopus laevis, alpha7 Nicotinic Acetylcholine Receptor, Cholinergic Antagonists metabolism, Cholinergic Antagonists pharmacology, Maze Learning drug effects, Memory drug effects, Phosphatidylcholines pharmacology, Receptors, Nicotinic metabolism, Scopolamine metabolism, Scopolamine pharmacology
- Abstract
Aims: The present study was conducted to understand the role of 1,2-dilynoleoyl-sn-glycero-3-phosphocholine (DLPhtCho) in cognitive functions., Main Methods: Two-electrode voltage-clamp was made to Xenopus oocytes expressing rat alpha7 acetylcholine (ACh) receptors. Field excitatory postsynaptic potentials (fEPSPs) were monitored from the CA1 region of rat hippocampal slices. Water maze test was carried out to assess spatial learning and memory for rats., Key Findings: In the oocyte expression system, DLPhtCho at a concentration of 10 microM potentiated ACh-evoked currents to approximately 190% of basal amplitudes 70 min after 10-min treatment. In contrast, 1-stearoyl-2-lynoleoyl-sn-glycero-3-phosphocholine (SLPhtCho), 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPhtCho), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPhtCho) had no effect on the currents. DLPhtCho (10 microM) enhanced slope of fEPSPs to about 150% of basal levels at 70-min treatment, that is inhibited by alpha-bungarotoxin, an inhibitor of alpha7 ACh receptors, while no enhancement was obtained with SLPhtCho, PLPhtCho, or POPhtCho. In the water maze test, oral administration with DLPhtCho (5 mg/kg) significantly shortened the prolonged acquisition latency for rats intraperitoneally injected with scopolamine (1 mg/kg)., Significance: The results of the present study show that DLPhtCho improves scopolamine-induced learning and memory deficits, possibly by facilitating hippocampal synaptic transmission under the control of alpha7 ACh receptors. DLPhtCho, therefore, could be developed as a beneficial anti-dementia drug.
- Published
- 2009
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14. The phosphodiesterase III inhibitor olprinone inhibits hippocampal glutamate release via a cGMP/PKG pathway.
- Author
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Fujikawa H, Kanno T, Nagata T, and Nishizaki T
- Subjects
- Animals, Calcium metabolism, Carbazoles pharmacology, Cyclic GMP-Dependent Protein Kinases antagonists & inhibitors, Excitatory Postsynaptic Potentials drug effects, Hippocampus metabolism, In Vitro Techniques, Isoquinolines pharmacology, Male, Membrane Potentials physiology, Phosphodiesterase 3 Inhibitors, Potassium metabolism, Protein Kinase Inhibitors pharmacology, Pyramidal Cells drug effects, Pyramidal Cells metabolism, Rats, Rats, Wistar, Receptors, AMPA metabolism, Sulfonamides pharmacology, Cyclic GMP metabolism, Cyclic GMP-Dependent Protein Kinases metabolism, Glutamic Acid metabolism, Hippocampus drug effects, Imidazoles pharmacology, Phosphodiesterase Inhibitors pharmacology, Pyridones pharmacology, Signal Transduction drug effects
- Abstract
Olprinone, an inhibitor of cyclic nucleotide phosphodiesterase III, inhibited an increase in intracellular Ca(2+) concentrations for acutely dissociated rat hippocampal pyramidal neurons induced by extracellular high K(+) (35 mM) depolarization. Olprinone (100 microM) significantly reduced spontaneous glutamate release from rat hippocampal slices. Furthermore, olprinone significantly decreased the rate of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor-mediated miniature excitatory postsynaptic currents (AMPA-mEPSCs) monitored from CA1 pyramidal neurons of rat hippocampal slices, and the effect was blocked by KT5823, an inhibitor of protein kinase G (PKG), but not by H-89, an inhibitor of protein kinase A (PKA). In the PKA assay using PC-12 cells, olprinone did not activate PKA. Taken together, the results of the present study show that olprinone attenuates intracellular Ca(2+) rise through voltage-sensitive Ca(2+) channels and inhibits presynaptic glutamate release via a cGMP/PKG pathway.
- Published
- 2008
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15. A new amyloid beta variant favoring oligomerization in Alzheimer's-type dementia.
- Author
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Tomiyama T, Nagata T, Shimada H, Teraoka R, Fukushima A, Kanemitsu H, Takuma H, Kuwano R, Imagawa M, Ataka S, Wada Y, Yoshioka E, Nishizaki T, Watanabe Y, and Mori H
- Subjects
- Adult, Aged, Alzheimer Disease diagnosis, Amyloid beta-Peptides genetics, Asian People genetics, Female, Genetic Markers genetics, Haplotypes genetics, Humans, Male, Middle Aged, Mutation genetics, Pedigree, Alzheimer Disease genetics, Amyloid beta-Protein Precursor genetics, Genetic Variation genetics
- Abstract
Objective: Soluble oligomers of amyloid beta (Abeta), rather than amyloid fibrils, have been proposed to initiate synaptic and cognitive dysfunction in Alzheimer's disease (AD). However, there is no direct evidence in humans that this mechanism can cause AD. Here, we report a novel amyloid precursor protein (APP) mutation that may provide evidence to address this question., Methods: A Japanese pedigree showing Alzheimer's-type dementia was examined for mutations in APP, PSEN1, and PSEN2. In addition, 5,310 Japanese people, including 2,121 patients with AD, were screened for the novel APP mutation. The pathogenic effects of this mutation on Abeta production, degradation, aggregation, and synaptotoxicity were also investigated., Results: We identified a novel APP mutation (E693Delta) producing variant Abeta lacking gulutamate-22 (E22Delta) in Japanese pedigrees showing Alzheimer's-type dementia and AD. Although the secretion of total Abeta was markedly reduced by this mutation, the variant Abeta was more resistant to proteolytic degradation. The mutant peptides showed the unique aggregation property of enhanced oligomerization but no fibrillization, and inhibited hippocampal long-term potentiation more potently than wild-type peptide in rats in vivo. Consistent with the nonfibrillogenic property of the variant Abeta, a very low amyloid signal was observed in the patient's brain on positron emission tomography using Pittsburgh compound-B., Interpretation: The E693Delta mutation has been suggested as a cause of dementia because of enhanced formation of synaptotoxic Abeta oligomers. Our findings may provide genetic validation in humans for the emerging hypothesis that the synaptic and cognitive impairment in AD is primarily caused by soluble Abeta oligomers.
- Published
- 2008
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16. Beneficial effect of intracellular free high-mannose oligosaccharides on cryopreservation of mammalian cells and proteins.
- Author
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Watanabe K, Yaguchi T, Yang D, Kanno T, Nagai K, Yamamoto S, Fujikawa H, Yamamoto H, Nagata T, Tashiro C, and Nishizaki T
- Subjects
- 1-Deoxynojirimycin pharmacology, Animals, Cell Survival drug effects, Cryoprotective Agents pharmacology, Enzyme Inhibitors pharmacology, Intracellular Fluid metabolism, Mannans pharmacology, Mannose chemistry, Oligosaccharides chemistry, PC12 Cells, Protein Kinase C-epsilon metabolism, Rats, alpha-Mannosidase antagonists & inhibitors, Cryopreservation, Mannans metabolism, Oligosaccharides metabolism
- Abstract
The cryoprotective effect of intracellular free high-mannose oligosaccharides (HMOS) on mammalian cells and proteins was examined by monitoring PC-12 cell viability and assaying protein kinase C (PKC)-epsilon activity. 1-Deoxymannojirimycin, an inhibitor of alpha-mannosidase, to cause an increase in intracellular free HMOS, significantly rescued PC-12 cells with 2-h freezing insult at -15 degrees C in a concentration (1-50mM)- and pretreatment time (48-72h)-dependent manner, as compared with unpretreated cells; full rescue from freezing injury was obtained with 1-deoxymannojirimycin at more than 25mM for 48-h pretreatment and more than 3mM for 72- and 96-h pretreatment. For PC-12 cells pretreated with 1-deoxymannojirimycin at 1mM for 72h, thawed cell viability after more than 8-w cryopreservation at -80 degrees C in 10% (v/v) dimethyl sulfoxide was much higher than that for cells without pretreatment. PKC-epsilon activity was well preserved after 16-h cryopreservation at -20 degrees C in the presence of mannose 9-N-acetylglucosamine 2 (Man9-GlcNAc2) (1 mM), an HMOS, while the activity was reduced to 15% without Man9-GlcNAc2. Collectively, the results of the present study suggest that intracellular free HMOS is a key molecule to protect mammalian cells and proteins from freezing injury; in other words, HMOS could be a new target for cryopreservation of mammalian cells and proteins.
- Published
- 2006
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17. A(1) adenosine receptor signal and AMPK involving caspase-9/-3 activation are responsible for adenosine-induced RCR-1 astrocytoma cell death.
- Author
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Sai K, Yang D, Yamamoto H, Fujikawa H, Yamamoto S, Nagata T, Saito M, Yamamura T, and Nishizaki T
- Subjects
- AMP-Activated Protein Kinases, Adenosine pharmacology, Analysis of Variance, Animals, Cell Death drug effects, Cell Line, Tumor, Dose-Response Relationship, Drug, Drug Interactions, Enzyme Activation drug effects, Enzyme Inhibitors pharmacology, Flow Cytometry methods, Immunohistochemistry methods, Mitochondrial Membranes drug effects, Models, Biological, RNA, Messenger metabolism, Rats, Reverse Transcriptase Polymerase Chain Reaction methods, Tetrazolium Salts, Theophylline analogs & derivatives, Theophylline pharmacology, Thiazoles, Adenosine physiology, Astrocytoma physiopathology, Caspases metabolism, Multienzyme Complexes physiology, Protein Serine-Threonine Kinases physiology, Receptor, Adenosine A1 physiology, Signal Transduction physiology
- Abstract
Extracellular adenosine reduced viability of RCR-1 rat astrocytoma cells in a dose (0.3-10mM)- and treatment time (24-72h)-dependent manner. In the apoptosis assay using propidium iodide (PI) and annexin V, treatment with adenosine (1mM) for 72h increased the population of PI-negative/annexin V-positive cells, that is related to early apoptosis, and that of PI-positive/annexin V-positive cells, that is related to late apoptosis/secondary necrosis. In addition, nuclei of cells treated with adenosine (1mM) for 72h were reactive to an antibody against single-stranded DNA. Adenosine activated caspase-3, -8 and -9, but mitochondrial membrane potentials were not affected. Adenosine-induced RCR-1 cell death was significantly inhibited by 8-CPT, an antagonist of A(1) adenosine receptors, and forskolin, an adenylate cyclase activator. SQ22536, an adenylate cyclase inhibitor, alternatively, exhibited an effect similar to adenosine. CHA, an agonist of A(1) adenosine receptors, activated caspase-3 and -9, but not caspase-8. Adenosine-induced cytotoxicity of RCR-1 cells was also significantly inhibited by dipyridamole, an inhibitor of adenosine transporter, and AMDA, an inhibitor of adenosine kinase. AICAR, an activator of AMP-activated protein kinase (AMPK), reduced RCR-1 cell viability, but synergistic effect was not obtained with co-treatment with adenosine and AICAR. AICAR activated caspase-3 and -9, but not caspase-8. An additive inhibition was found in the co-presence of 8-CPT and dipyridamole. Extracellular adenosine, thus, appears to activate caspase-9 followed by the effector caspase, caspase-3, at least via two independent pathways linked to A(1) adenosine receptor-mediated adenylate cyclase inhibition and adenosine uptake into cells/conversion to AMP/activation of AMPK, possibly regardless of mitochondrial damage, thereby leading to RCR-1 cell death, dominantly by apoptosis. Moreover, caspase-8 activation could again contribute to adenosine-induced cytotoxicity, although the underlying mechanism is currently unknown. Collectively, the results of the present study may represent a new pathway for caspase activation relevant to diverse adenosine signals in cell death.
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- 2006
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18. The linoleic acid derivative DCP-LA selectively activates PKC-epsilon, possibly binding to the phosphatidylserine binding site.
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Kanno T, Yamamoto H, Yaguchi T, Hi R, Mukasa T, Fujikawa H, Nagata T, Yamamoto S, Tanaka A, and Nishizaki T
- Subjects
- Animals, Base Sequence, Binding Sites, Caprylates metabolism, Cell Line, Enzyme Activation drug effects, Enzyme Inhibitors pharmacology, Humans, Isoenzymes antagonists & inhibitors, Isoenzymes genetics, Isoenzymes metabolism, Linoleic Acids chemistry, Linoleic Acids metabolism, Linoleic Acids pharmacology, PC12 Cells, Phosphatidylserines chemistry, Phosphatidylserines pharmacology, Protein Kinase C antagonists & inhibitors, Protein Kinase C genetics, Protein Kinase C metabolism, Protein Kinase C-epsilon antagonists & inhibitors, Protein Kinase C-epsilon genetics, RNA, Small Interfering genetics, Rats, Reverse Transcriptase Polymerase Chain Reaction, Substrate Specificity, Caprylates pharmacology, Phosphatidylserines metabolism, Protein Kinase C-epsilon metabolism
- Abstract
This study examined the effect of 8-[2-(2-pentyl-cyclopropylmethyl)-cyclopropyl]-octanoic acid (DCP-LA), a newly synthesized linoleic acid derivative with cyclopropane rings instead of cis-double bonds, on protein kinase C (PKC) activity. In the in situ PKC assay with reverse-phase high-performance liquid chromatography, DCP-LA significantly activated PKC in PC-12 cells in a concentration-dependent (10 nM-100 microM) manner, with the maximal effect at 100 nM, and the DCP-LA effect was blocked by GF109203X, a PKC inhibitor, or a selective inhibitor peptide of the novel PKC isozyme PKC-epsilon. Furthermore, DCP-LA activated PKC in HEK-293 cells that was inhibited by the small, interfering RNA against PKC-epsilon. In the cell-free PKC assay, of the nine isozymes examined here, DCP-LA most strongly activated PKC-epsilon, with >7-fold potency over other PKC isozymes, in the absence of dioleoyl-phosphatidylserine and 1,2-dioleoyl-sn-glycerol; instead, the DCP-LA action was inhibited by dioleoyl-phosphatidylserine. DCP-LA also activated PKC-gamma, a conventional PKC, but to a much lesser extent compared with that for PKC-epsilon, by a mechanism distinct from PKC-epsilon activation. Thus, DCP-LA serves as a selective activator of PKC-epsilon, possibly by binding to the phosphatidylserine binding site on PKC-epsilon. These results may provide fresh insight into lipid signaling in PKC activation.
- Published
- 2006
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19. Linoleic acid derivative DCP-LA improves learning impairment in SAMP8.
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Yaguchi T, Nagata T, Mukasa T, Fujikawa H, Yamamoto H, Yamamoto S, Iso H, Tanaka A, and Nishizaki T
- Subjects
- Aging genetics, Animals, Avoidance Learning drug effects, Behavior, Animal drug effects, Disease Models, Animal, Exploratory Behavior drug effects, Learning Disabilities etiology, Male, Maze Learning drug effects, Mice, Mice, Neurologic Mutants, Motor Activity drug effects, Reaction Time drug effects, Aging physiology, Caprylates therapeutic use, Learning Disabilities drug therapy, Linoleic Acid chemistry
- Abstract
In the water-maze test, the linoleic acid derivative, 8-[2-(2-pentyl-cyclopropylmethyl)-cyclopropyl]-octanoic acid (DCP-LA) (1 mg/kg, intraperitoneally), significantly shortened the prolonged latency for accelerated-senescence-prone mice 8 (SAMP8), reaching a level similar to the latency for accelerated-senescence-resistant mice 1 (SAMR1) as control. In the open-field test to assess motor activity, it was confirmed that the DCP-LA effect is not due to increased motor activity. In the passive avoidance test to assess fear memory, DCP-LA had no effect on the latency of acquisition and retention for SAMP8. The results of the present study, thus, suggest that DCP-LA could improve age-related learning impairment by enhancing cognitive functions.
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- 2006
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20. Modulation of P2X receptors via adrenergic pathways in rat dorsal root ganglion neurons after sciatic nerve injury.
- Author
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Maruo K, Yamamoto H, Yamamoto S, Nagata T, Fujikawa H, Kanno T, Yaguchi T, Maruo S, Yoshiya S, and Nishizaki T
- Subjects
- Adaptation, Physiological, Animals, Cells, Cultured, Male, Rats, Rats, Wistar, Receptors, Adrenergic, Receptors, Purinergic P2X, Afferent Pathways metabolism, Ganglia, Spinal metabolism, Norepinephrine metabolism, Posterior Horn Cells metabolism, Receptors, Purinergic P2 metabolism, Sciatic Nerve injuries, Sciatic Nerve metabolism
- Abstract
The present study examined noradrenaline-induced modulation of ATP-evoked currents in dorsal root ganglion (DRG) neurons after sciatic nerve injury (transection). ATP (10 microM) generated fast/mixed type of whole-cell membrane currents, possibly as mediated via P2X(3)/P2X(3)-like receptors, and slow type of the currents, possibly as mediated via P2X(2/3) receptors, in acutely dissociated L4/5 DRG neurons, without significant difference between sham and injury group. For sham group, noradrenaline (10 microM) enhanced fast/mixed type of ATP-evoked currents in ipsilateral DRG neurons, that is not inhibited by H-7, a broad inhibitor of protein kinases, but otherwise it had no effect on slow type of the currents. For injury group, noradrenaline (10 microM) significantly potentiated slow type of ATP-evoked currents in ipsilateral DRG neurons, that is abolished by H-7 or GF109203X, a selective inhibitor of protein kinase C (PKC), while it depressed fast/mixed type of the currents. In the analysis of real-time reverse transcription-polymerase chain reaction, an increase in the mRNAs for alpha(1b), alpha(2a), alpha(2d), and beta(2) adrenergic receptors was found with the ipsilateral DRGs after sciatic nerve injury. Collectively, the results of the present study suggest that noradrenaline potentiates P2X(2/3) receptor currents by activating PKC via alpha(1) adrenergic receptors linked to G(q) protein, perhaps dominantly alpha(1b) adrenergic receptors, in DRG neurons after sciatic nerve injury. This may account for a nociceptive pathway in response to noradrenergic sprouting after peripheral nerve injury.
- Published
- 2006
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21. Bidirectional regulations for glutamate and GABA release in the hippocampus by alpha7 and non-alpha7 ACh receptors.
- Author
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Kanno T, Yaguchi T, Yamamoto S, Nagata T, Yamamoto H, Fujikawa H, and Nishizaki T
- Subjects
- Animals, Cells, Cultured, Male, Neurotransmitter Agents metabolism, Rats, Rats, Wistar, alpha7 Nicotinic Acetylcholine Receptor, Gene Expression Regulation physiology, Glutamic Acid metabolism, Hippocampus metabolism, Neurons metabolism, Receptors, Nicotinic metabolism, gamma-Aminobutyric Acid metabolism
- Abstract
In the assay of glutamate and gamma-aminobutyric acid (GABA) with a high-performance liquid chromatography, spontaneous release of glutamate and GABA from rat hippocampal slices was significantly enhanced by mecamylamine, an inhibitor of non-alpha7 ACh receptors, or alpha-bungarotoxin, an inhibitor of alpha7 ACh receptors in the absence of tetrodotoxin (TTX), but not in the presence of TTX. Nicotine significantly enhanced glutamate and GABA release in the absence of TTX, that is abolished by mecamylamine or alpha-bungarotoxin, while it had no effect on the release in the presence of TTX. In the recording of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor-mediated excitatory postsynaptic currents (AMPA-EPSCs) and GABA(A) receptor-mediated inhibitory postsynaptic currents (GABA(A)-IPSCs) from CA1 pyramidal neurons of rat hippocampal slices, nicotine did not affect the rate and amplitude of AMPA-EPSCs and AMPA-miniature EPSCs. In contrast, nicotine significantly increased the rate of GABA(A)-IPSCs, without affecting the amplitude, but such effect was not obtained with GABA(A)-miniature IPSCs. The collective results suggest that alpha7 and non-alpha7 ACh receptors expressed in the hippocampus, activated under the basal conditions, inhibit release of glutamate and GABA controlled through multi-synaptic relays, but that otherwise, those receptors, highly activated by nicotine, stimulate both the release, with a part of GABA released from interneurons transmitting to CA1 pyramidal neurons. Furthermore, the results also suggest that alpha7 and non-alpha7 ACh receptors do not have potency sufficiently to modulate glutamate and GABA release controlled by single synapses.
- Published
- 2005
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22. 8-[2-(2-pentyl-cyclopropylmethyl)-cyclopropyl]-octanoic acid stimulates GABA release from interneurons projecting to CA1 pyramidal neurons in the rat hippocampus via pre-synaptic alpha7 acetylcholine receptors.
- Author
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Kanno T, Yaguchi T, Yamamoto S, Yamamoto H, Fujikawa H, Nagata T, Tanaka A, and Nishizaki T
- Subjects
- Animals, Enzyme Inhibitors pharmacology, Hippocampus cytology, Hippocampus physiology, Indoles pharmacology, Interneurons metabolism, Male, Maleimides pharmacology, Membrane Potentials drug effects, Neural Pathways, Oocytes physiology, Organ Culture Techniques, Patch-Clamp Techniques, Presynaptic Terminals metabolism, Protein Kinase C metabolism, Rats, Rats, Wistar, Xenopus, alpha7 Nicotinic Acetylcholine Receptor, Caprylates pharmacology, Hippocampus drug effects, Interneurons cytology, Interneurons drug effects, Pyramidal Cells cytology, Receptors, Nicotinic metabolism, gamma-Aminobutyric Acid metabolism
- Abstract
Nicotinic acetylcholine (ACh) receptors, such as alpha7, alpha3beta4 and alpha4beta2 receptors in the hippocampus, are suggested to modulate neurotransmitter release. 8-[2-(2-Pentyl-cyclopropylmethyl)-cyclopropyl]-octanoic acid (DCP-LA) (100 nM), a linoleic acid derivative, potentiated responses of alpha7, alpha3beta4 and alpha4beta2 ACh receptors expressed in Xenopus oocytes that are blocked by 3-(1-[dimethylaminopropyl] indol-3-yl)-4-[indol-3-yl] maleimide (GF109203X), a selective inhibitor of protein kinase C (PKC), except for alpha3beta4 ACh receptors. DCP-LA enhanced the nicotine-triggered release of GABA from rat hippocampal slices in the presence of tetrodotoxin in a bell-shaped dose-dependent manner at concentrations ranging from 10 nM to 10 microM, although DCP-LA by itself had no effect on GABA release. The DCP-LA action was inhibited by GF109203X or alpha-bungarotoxin, an inhibitor of alpha7 ACh receptors, but not by mecamylamine or dihydro-beta-erithroidine, an inhibitor of alpha3beta4 and alpha4beta2 ACh receptors. A similar effect on GABA release was obtained with 12-O-tetradecanoylphorbol 13-acetate, a PKC activator. DCP-LA (100 nM) also enhanced GABA release triggered by choline, an agonist of alpha7 ACh receptors, but not 3-[2(s)-azetidinylmethoxy] pyridine, an agonist of alpha4beta2 ACh receptors. In addition, DCP-LA (100 nM) increased the rate of nicotine-triggered GABA(A) receptor-mediated miniature inhibitory post-synaptic currents, monitored from CA1 pyramidal neurons of rat hippocampal slices, and the effect was also inhibited by GF109203X or alpha-bungarotoxin but not by mecamylamine. Thus, the results of the present study indicate that DCP-LA stimulates GABA release by enhancing activity of pre-synaptic alpha7 ACh receptors present on the GABAergic terminals of interneurons that transmit to CA1 pyramidal neurons via a PKC pathway.
- Published
- 2005
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23. Effects of cis-unsaturated free fatty acids on PKC-epsilon activation and nicotinic ACh receptor responses.
- Author
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Yaguchi T, Yamamoto S, Nagata T, Kanno T, Tanaka A, and Nishizaki T
- Subjects
- Animals, Chromatography, High Pressure Liquid methods, Linoleic Acid pharmacology, Linoleic Acids pharmacology, Macromolecular Substances, Membrane Potentials drug effects, Microinjections methods, Oocytes, Patch-Clamp Techniques methods, Phosphorylation drug effects, Protein Kinase C-epsilon, Torpedo, Xenopus laevis, Enzyme Activation drug effects, Fatty Acids, Unsaturated pharmacology, Protein Kinase C metabolism, Receptors, Nicotinic metabolism
- Abstract
Linoleic acid (C18:2omega6) (10 microM) depressed ACh-evoked whole-cell membrane currents followed by potentiation of the currents in Xenopus oocytes expressing Torpedo ACh receptors. The potentiation was never obtained with linoleoylamide (10 microM), which contains CONH2 instead of COOH on linoleic acid. In the assay of protein kinase C (PKC) using a reversed phase high-performance liquid chromatography, linoleic acid activated PKC-epsilon but linoleoylamide otherwise had no effect on it. Of a variety of cis-unsaturated free fatty acids with 20 hydrocarbon chains (C20), 8-eicosenoic acid (C20:1omega12) potentiated ACh receptor channel currents without depression. 11,14-Eicosadienoic acid (C20:2omega6), 5,8,11-eicosatrienoic acid (C20:3omega9), 5,8,11,14-eicosatetraenoic acid (arachidonic acid) (C20:4omega6), and 5,8,11,14,17-eicosapentaenoic acid (C20:5omega3) depressed and potentiated the currents. In contrast, only a depression was obtained with 5-eicosenoic acid (C20:1omega15), 11-eicosenoic acid (C20:1omega9), and 8,11,14-eicosatrienoic acid (C20:3omega6). All the free fatty acids (C20) involved PKC-epsilon activation, with no significant difference in the potency among them. Taken together, it appears that carboxyl on cis-unsaturated free fatty acids plays a significant role in PKC-epsilon activation and potentiation of nicotinic ACh receptor responses. The role of the number and position of the cis-double bonds in the receptor responses, however, remains an open question.
- Published
- 2005
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24. Interleukin-18 stimulates synaptically released glutamate and enhances postsynaptic AMPA receptor responses in the CA1 region of mouse hippocampal slices.
- Author
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Kanno T, Nagata T, Yamamoto S, Okamura H, and Nishizaki T
- Subjects
- Animals, Excitatory Postsynaptic Potentials physiology, Hippocampus metabolism, In Vitro Techniques, Male, Mice, Mice, Inbred C57BL, Glutamic Acid metabolism, Hippocampus physiology, Interleukin-18 pharmacology, Receptors, AMPA metabolism, Synapses metabolism
- Abstract
The present study examined the effects of the proinflammatory cytokine interleukin-18 (IL-18) on mouse hippocampal synaptic transmission. IL-18 (100 ng/ml) significantly increased amplitude and frequency of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor-mediated miniature excitatory postsynaptic currents (AMPA-mEPSCs), that are monitored from CA1 pyramidal neurons of mouse hippocampal slices. IL-18 (100 ng/ml) enhanced slope of basal field excitatory postsynaptic potentials (fEPSPs) that are recorded from the CA1 region of mouse hippocampal slices. There was no significant difference in the expression of Schaffer collateral/CA1 long-term potentiation (LTP) between in the presence and absence of IL-18, although IL-18 tended to inhibit saturation levels of the potentiation induced by tetanic stimulation in a dose-dependent manner at concentrations ranged from 10 ng/ml to 1 microg/ml. Paired-pulse facilitation in the presence of IL-18 (100 ng/ml) was not influenced after tetanic stimulation, while that in the absence of IL-18 was depressed. The results of the present study, thus, suggest that IL-18 stimulates synaptically released glutamate and enhances postsynaptic AMPA receptor responses in CA1 pyramidal neurons of mouse hippocampal slices, thereby facilitating basal hippocampal synaptic transmission without affecting the LTP., (Copyright 2004 Elsevier B.V.)
- Published
- 2004
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25. 2-pyrrolidinone induces a long-lasting facilitation of hippocampal synaptic transmission by enhancing alpha7 ACh receptor responses via a PKC pathway.
- Author
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Miyamoto H, Yaguchi T, Ohta K, Nagai K, Nagata T, Yamamoto S, and Nishizaki T
- Subjects
- Acetylcholine pharmacology, Animals, Blotting, Western, Bungarotoxins pharmacology, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Calcium-Calmodulin-Dependent Protein Kinases metabolism, Chromatography, High Pressure Liquid, Dose-Response Relationship, Drug, Drug Interactions, Electric Stimulation, Enzyme Inhibitors pharmacology, Excitatory Postsynaptic Potentials drug effects, Hippocampus anatomy & histology, Hippocampus physiology, In Vitro Techniques, Membrane Potentials drug effects, Oocytes, Patch-Clamp Techniques instrumentation, Patch-Clamp Techniques methods, Protein Kinase C antagonists & inhibitors, Rats, Xenopus, alpha7 Nicotinic Acetylcholine Receptor, Hippocampus drug effects, Protein Kinase C metabolism, Pyrrolidinones pharmacology, Receptors, Nicotinic metabolism, Synaptic Transmission drug effects
- Abstract
2-Pyrrolidinone, a metabolite of aniracetam, potentiated currents through alpha7 receptors expressed in Xenopus oocytes, in a bell-shaped dose-dependent manner at concentrations ranged from 1 nM to 10 microM, with a maximum at 100 nM (189% of original levels 60 min after 20-min treatment). The potentiation was inhibited by GF109203X, a selective inhibitor of protein kinase C (PKC), but not by KN-93, a selective inhibitor of CaMKII, or H-89, a selective inhibitor of protein kinase A (PKA). In the PKC assay using reversed-phase high-performance liquid chromatography, 2-pyrrolidinone enhanced activity of PKC-epsilon activated by linoleic acid to about 1.8-times greater than that in the absence of 2-pyrrolidinone, although it did not directly activate PKC-epsilon. In the Western immunoblot analysis, rat hippocampal slices treated with 2-pyrrolidinone (100 nM) was more reactive to an antibody against phosphorylated myristoylated alanine-rich C kinase substrate (MARCKS) than untreated slices. 2-Pyrrolidinone (100 nM) induced a long-lasting facilitation of hippocampal synaptic transmission in the CA1 region of rat hippocampal slices, and the facilitation was inhibited by GF109203X or alpha-bungarotoxin, an inhibitor of alpha7 receptors. The results of the present study suggest that 2-pyrrolidinone enhances activity of activated PKC, thereby potentiating alpha7 receptor responses, and then leading to a facilitation of hippocampal synaptic transmission.
- Published
- 2003
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26. Tunicamycin inhibits NMDA and AMPA receptor responses independently of N-glycosylation.
- Author
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Maruo K, Nagata T, Yamamoto S, Nagai K, Yajima Y, Maruo S, and Nishizaki T
- Subjects
- Animals, Drug Interactions, Electric Stimulation, Excitatory Amino Acid Agonists pharmacology, Glycosylation drug effects, Hippocampus drug effects, Hippocampus physiology, In Vitro Techniques, Kainic Acid pharmacology, Long-Term Potentiation drug effects, Membrane Potentials drug effects, N-Methylaspartate pharmacology, Neurons drug effects, Neurons physiology, Patch-Clamp Techniques, Rats, Receptors, AMPA physiology, Receptors, N-Methyl-D-Aspartate physiology, Time Factors, Anti-Bacterial Agents pharmacology, Receptors, AMPA antagonists & inhibitors, Receptors, N-Methyl-D-Aspartate antagonists & inhibitors, Tunicamycin pharmacology
- Abstract
In a whole-cell patch-clamp configuration, currents through N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor channels were monitored in cultured rat hippocampal neurons, and those currents were depressed to 25 and 28% of basal levels, respectively, by 3-min treatment with tunicamycin (10 microM), an inhibitor of protein N-glycosylation. Tunicamycin (10 microM) reduced amplitude of population spikes elicited in the dentate gyrus of rat hippocampal slices, reaching 78% of basal levels 60 min after the beginning of treatment, and long-term potentiation (LTP) of the perforant path was never induced in the presence of tunicamycin. Tunicamycin, thus, appears to serve as a modulator for NMDA and AMPA receptors, regardless of N-glycosylation, thereby inhibiting neurotransmission and LTP in the dentate gyrus.
- Published
- 2003
- Full Text
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27. (-)-Epigallocatechin gallate protects against NO stress-induced neuronal damage after ischemia by acting as an anti-oxidant.
- Author
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Nagai K, Jiang MH, Hada J, Nagata T, Yajima Y, Yamamoto S, and Nishizaki T
- Subjects
- Animals, Brain Ischemia metabolism, Brain Ischemia physiopathology, Cell Survival drug effects, Cells, Cultured, Cerebrovascular Circulation drug effects, Hippocampus metabolism, Neurons metabolism, Nitric Oxide Donors pharmacology, Nitroprusside pharmacology, Rats, Rats, Wistar, Antioxidants pharmacology, Brain Ischemia drug therapy, Catechin analogs & derivatives, Catechin pharmacology, Hippocampus drug effects, Neurons drug effects, Neuroprotective Agents pharmacology, Nitric Oxide adverse effects, Oxidative Stress drug effects
- Abstract
The present study investigated the effects of (-)-epigallocatechin gallate (EGCG), which is the major component of polyphenol in green tea, on nitric oxide (NO) stress-induced neuronal damage, by monitoring NO mobilizations in the intact rat hippocampus and assaying the viability of cultured rat hippocampal neurons. A 10-min ischemia increased NO (NO(3)(-)/NO(2)(-)) concentrations in the intact rat hippocampus, while EGCG (50 mg/kg i.p.) inhibited the increase by 77% without affecting hippocampal blood flow. The NO donor, sodium nitroprusside (SNP; 50 microM), produced NO (NO(3)(-)/NO(2)(-)), while EGCG inhibited it in a dose-dependent manner at concentrations ranging from 50 to 200 microM. Treatment with SNP (100 microM) reduced the viability of cultured rat hippocampal neurons to 22% of control levels, while EGCG caused it to recover to 51% for 10 microM, 73% for 20 microM, and 70% for 50 microM. Taken together, it appears that EGCG could protect against ischemic neuronal damage by deoxidizing peroxynitrate/peroxynitrite, which is converted to NO radical or hydroxy radical., (Copyright 2002 Elsevier Science B.V.)
- Published
- 2002
- Full Text
- View/download PDF
28. The inhibitory and facilitatory actions of amyloid-beta peptides on nicotinic ACh receptors and AMPA receptors.
- Author
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Tozaki H, Matsumoto A, Kanno T, Nagai K, Nagata T, Yamamoto S, and Nishizaki T
- Subjects
- Amyloid beta-Peptides administration & dosage, Animals, Oocytes drug effects, Oocytes metabolism, Peptide Fragments administration & dosage, Receptors, AMPA metabolism, Receptors, Nicotinic metabolism, Torpedo, Xenopus, alpha7 Nicotinic Acetylcholine Receptor, Amyloid beta-Peptides pharmacology, Peptide Fragments pharmacology, Receptors, AMPA drug effects, Receptors, Nicotinic drug effects
- Abstract
The present study investigated the effects of amyloid-beta peptides on nicotinic ACh receptors (Torpedo, alpha 4 beta 2, and alpha 7 receptors) and AMPA receptors expressed in Xenopus oocytes by monitoring whole-cell membrane currents. Ten-minutes treatment with amyloid-beta(1-42) (1 microM) inhibited Torpedo ACh receptor currents, reaching 53% of original levels 30 min after treatment. Amyloid-beta(1-40) inhibited the currents in a dose-dependent manner (0.1-10 microM) during treatment, gradually reversing after treatment. Amyloid-beta(1-40) and amyloid-beta(1-42) (0.1 microM) depressed alpha 4 beta 2 receptor currents to each 69% and 62% of original levels at 10-min treatment and lesser depression was obtained with alpha 7 receptors. Amyloid-beta(1-42) (0.1 microM) did not significantly inhibit AMPA receptor currents, but amyloid-beta(1-40) (0.1 microM) potentiated the currents to 145-191% of original levels. Amyloid-beta peptides, thus, exert their diverse actions on nicotinic ACh receptors and AMPA receptors, and the inhibitory actions on nicotinic ACh receptors may account for the deterioration of learning and memory in Alzheimer's disease.
- Published
- 2002
- Full Text
- View/download PDF
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