1. Three dimensional excitation-emission matrix fluorescence spectroscopy of typical Japanese soil powders.
- Author
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Nakaya Y, Nakashima S, Moriizumi M, Oguchi M, Kashiwagi S, and Naka N
- Abstract
Front face fluorescence spectroscopy of typical Japanese soil powders (soil A: Typic Hapludand; soil H: Typic Hydraquent; soil Y: Typic Paleudult) has been conducted. Three dimensional excitation-emission matrix fluorescence spectra of the 100 wt% soils showed similar fluorescence patterns to each other. The fluorescence patterns were similar between the soil samples and their residues after extraction by NaOH solution for 60 min. In order to examine fluorescence extinction from a view point of whiteness of the soils, the soil powders were mixed with white and black diluents (Al
2 O3 and Fe3 O4 ) and fluorescence spectra of the mixtures were measured at 450 nm excitation. At low levels of dilution with Al2 O3 (2-100 wt% of A; 50-100 wt% of H and Y), the fluorescence intensities increased with dilution. At high levels of dilution with Al2 O3 , the fluorescence intensities decreased with dilution. On the other hand, fluorescent intensities decreased by dilution with Fe3 O4 . These results suggested inner filter effect-like fluorescence extinction by (1) large amount of blackish organic compounds giving high total carbon value and (2) blackish non-fluorescent mineral compounds. In order to correct the fluorescence intensities of the mixtures containing the sample soils and the diluents, we preliminary applied a correction method based on the Kubelka-Munk theory using diffuse reflectance. The corrected fluorescence intensities of samples with white diluents (Al2 O3 ) were described by a simple fluorescence response model having saturation values., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
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