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Regenerative agricultural practice adoption on conventionally managed fields has gained momentum as a climate mitigation strategy, given the ability of these practices to sequester carbon or reduce greenhouse gas emissions. However, the geospatial and temporal variability of the impact of specific practices, such as cover cropping or no-till, pose challenges for scalable quantification of emissions reduction and deploying incentives to drive increased adoption. To quantify impact while accounting for variability and uncertainty at scale, Indigo Ag created a monitoring, reporting, and verification (MRV) pipeline to produce agricultural soil carbon credits produced at large scales (hundreds of thousands of hectares). The pipeline ingests field data from enrolled farmers, checks data quality, uses hybrid soil sampling and biogeochemical modeling to produce estimates of emissions reduction and uncertainty, and then applies deductions based on calculated uncertainty and leakage to quantify total project-wide carbon credits and monitor for durability of carbon. The implementation of a carbon project (CAR1459) from 2018 to 2022 on 553,743 ha of U.S. cropland utilizing the pipeline is estimated to have reduced emissions by 398,408.5 tCO 2 e, amounting to 296,662 tCO2e of soil carbon credits after uncertainty deductions. This paper explores the effect sizes associated with specific regenerative practice changes across the project domain. Cover cropping consistently resulted in a net positive climate impact and reduced emissions by 1.29 tCO 2 e per hectare per year, on average. Introduction of no-till was more common in the project, but it had a lower average emissions reduction of 0.38 tCO 2 e per hectare per year. Effect sizes for no-till vary spatiotemporally and are typically low in the first several years after adoption but increase in subsequent years. Agricultural carbon programs that capture and incentivize the nuance of outcomes of practices rather than the implementation of practices, can promote adoption of the right management practice to be deployed on the right field for maximum environmental benefit., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Mark Easter reports financial support was provided by Indigo Ag, Inc. Mark A. Friedl reports financial support was provided by Indigo Ag, Inc. Ashok A. Kumar reports a relationship with Indigo Ag, Inc. that includes: employment and equity or stocks. Mark Easter reports a relationship with Indigo Ag, Inc. that includes: consulting or advisory, employment, and equity or stocks. Mark A. Friedl reports a relationship with Indigo Ag, Inc. that includes: consulting or advisory. Charles D. Brummitt reports a relationship with Indigo Ag, Inc. that includes: employment and equity or stocks. Cara A. Mathers reports a relationship with Indigo Ag, Inc. that includes: employment and equity or stocks. Rebecca Arundale Keating reports a relationship with Indigo Ag, Inc. that includes: employment and equity or stocks. Katherine O'Leary reports a relationship with Indigo Ag, Inc. that includes: employment and equity or stocks. Eleanor E. Campbell reports a relationship with Indigo Ag, Inc. that includes: employment and equity or stocks. Max DuBuisson reports a relationship with Indigo Ag, Inc. that includes: employment and equity or stocks. Ryan Pape reports a relationship with Indigo Ag, Inc. that includes: employment and equity or stocks. Samuel J.W. Peters reports a relationship with Indigo Ag, Inc. that includes: employment and equity or stocks. Ashok A. Kumar has patent issued to Indigo Ag, Inc. Eleanor E. Campbell has patent issued to Indigo Ag, Inc. Samuel J.W. Peters has patent issued to Indigo Ag, Inc. Charles D. Brummitt has patent issued to Indigo Ag, Inc. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Non-communicable diseases (NCDs) pose a global health challenge, leading to substantial morbidity, mortality, and economic strain. Our review underscores the escalating incidence of NCDs worldwide and highlights the potential of regenerative agriculture (RA) products in mitigating these diseases. We also explore the efficacy of dietary interventions in NCD management and prevention, emphasizing the superiority of plant-based diets over those high in processed foods and red meat. Examining the role of the gut microbiome in various diseases, including liver disorders, allergies, metabolic syndrome, inflammatory bowel disease, and colon cancer, we find compelling evidence implicating its influence on disease development. Notably, dietary modifications can positively affect the gut microbiome, fostering a symbiotic relationship with the host and making this a critical strategy in disease prevention and treatment. Investigating agricultural practices, we identify parallels between soil/plant and human microbiome studies, suggesting a crucial link between soil health, plant- and animal-derived food quality, and human well-being. Conventional/Industrial agriculture (IA) practices, characterized in part by use of chemical inputs, have adverse effects on soil microbiome diversity, food quality, and ecosystems. In contrast, RA prioritizes soil health through natural processes, and includes avoiding synthetic inputs, crop rotation, and integrating livestock. Emerging evidence suggests that food from RA systems surpasses IA-produced food in quality and nutritional value. Recognizing the interconnection between human, plant, and soil microbiomes, promoting RA-produced foods emerges as a strategy to improve human health and environmental sustainability. By mitigating climate change impacts through carbon sequestration and water cycling, RA offers dual benefits for human and planetary health and well-being. Emphasizing the pivotal role of diet and agricultural practices in combating NCDs and addressing environmental concerns, the adoption of regional RA systems becomes imperative. Increasing RA integration into local food systems can enhance food quality, availability, and affordability while safeguarding human health and the planet's future., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

There is an urgent need for global food systems transformation to realize a future where planetary health reaches its full potential. Paramount to this vision is the ability of stakeholders across sectors to understand how foods and dietary patterns impact food systems inclusive of all domains of sustainability-environmental, nutrition/health, economic and social. This article is a synopsis of presentations by 3 food systems experts to share the latest science in a session entitled "How do you measure sustainability? Opportunities for consistent and holistic metrics to support food systems transformation" at the American Society for Nutrition's 2023 annual conference. As summarized here, global population data showing widespread malnutrition underscore the important role of dietary diversity through a balance of plant- and animal-source foods to achieve nutritionally adequate diets and reduce risk of noncommunicable diseases. Yet, recent international audits of countries, companies, and organizations and their sustainability targets largely demonstrate an underrepresentation of robust nutrition/health metrics to support public nutrition and health progress. Addressing limitations in diet-sustainability modeling systems provides a viable opportunity to accurately reflect the important contributions and trade-offs of diets across all domains of sustainability to ultimately support evidence-based decision making in advancing healthy food systems., Competing Interests: ALU, MAP, and KB were employees of National Dairy Council at the time this article was written. The use of brands and/or images of branded products is intended only to provide examples of concepts being discussed and does not imply endorsement of any brand or product. ZC has research awards from the Jeffress Trust Awards Program in Research Advancing Health Equity and the USDA for projects unrelated to this article. The other author reports no conflicts of interest., (© 2024 The Author(s).)

Proponents of both the One Health and Planetary Health paradigms have acknowledged that current methods of agricultural food production are driving many environmental changes with negative human health consequences, including climate change, deforestation, and the emergence of zoonotic disease and antimicrobial resistance. Currently, the training of human health, veterinary, and public health professionals typically does not include aspects of soil health. Much of the resultant discussion in One Health and Planetary Health circles regarding interventions to address the health impact of agricultural practices has focused on measures such as advocating for dietary change toward plant based diets and increasing food safety, biosecurity, disease surveillance and antimicrobial stewardship. A greater understanding of soil health and its relationship to agricultural practices could prove foundational to many of the problems that the One Health and Planetary Health perspectives aim to address, including antimicrobial resistance, zoonotic disease emergence, food security, and climate change. A consequent global focus on the health of soils offers a promise of specific opportunities for preventive interventions and a greater convergence between the One Health and Planetary Health approaches., Competing Interests: One of the authors: (DRM) has published books on the topic of soil health., (© 2023 Published by Elsevier B.V.)

Redesigning agrosystems to include more ecological regulations can help feed a growing human population, preserve soils for future productivity, limit dependency on synthetic fertilizers, and reduce agriculture contribution to global changes such as eutrophication and warming. However, guidelines for redesigning cropping systems from natural systems to make them more sustainable remain limited. Synthetizing the knowledge on biogeochemical cycles in natural ecosystems, we outline four ecological systems that synchronize the supply of soluble nutrients by soil biota with the fluctuating nutrient demand of plants. This synchrony limits deficiencies and excesses of soluble nutrients, which usually penalize both production and regulating services of agrosystems such as nutrient retention and soil carbon storage. In the ecological systems outlined, synchrony emerges from plant-soil and plant-plant interactions, eco-physiological processes, soil physicochemical processes, and the dynamics of various nutrient reservoirs, including soil organic matter, soil minerals, atmosphere, and a common market. We discuss the relative importance of these ecological systems in regulating nutrient cycles depending on the pedoclimatic context and on the functional diversity of plants and microbes. We offer ideas about how these systems could be stimulated within agrosystems to improve their sustainability. A review of the latest advances in agronomy shows that some of the practices suggested to promote synchrony (e.g., reduced tillage, rotation with perennial plant cover, crop diversification) have already been tested and shown to be effective in reducing nutrient losses, fertilizer use, and N 2 O emissions and/or improving biomass production and soil carbon storage. Our framework also highlights new management strategies and defines the conditions for the success of these nature-based practices allowing for site-specific modifications. This new synthetized knowledge should help practitioners to improve the long-term productivity of agrosystems while reducing the negative impact of agriculture on the environment and the climate., (© 2023 The Authors. Global Change Biology published by John Wiley & Sons Ltd.)

Regenerative agriculture is an alternative approach to farming that has been gaining traction and interest among farmers due to its potential to reduce input costs, improve soil health, and increase the resilience of farming systems. This paper undertakes a practice-based analysis of farmers, applying a lens of regenerative agriculture. Surveys were developed as a part of a broader project using an established methodological framework. Topics were developed and adapted with input from local stakeholders before being mailed out to three farming regions across Australia (the Western Australian Wheatbelt, the Eyre Peninsula in South Australia and Central West New South Wales). The research clustered farmers into two groups: those who are using best-practices that fall inside the scope of regenerative agriculture, and those who are not. The similarities and differences in farmer attributes, as well as self-reported knowledge levels and information sources used by each group are explored. Results indicate that a belief in anthropogenic climate change may be one of the primary divides between the two groups, and therefore a possible driver of best-practice implementation. The findings provide insight into perceptions of regenerative agriculture for Australian farmers, and may assist with knowledge dissemination amongst those managing our environment., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr Hanabeth Luke reports financial support was provided by Cooperative Research Centre for High Performance Soils (Soil CRC). Mathew Alexanderson reports financial support was provided by Cooperative Research Centre for High Performance Soils (Soil CRC)., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The ongoing climate crisis merits an urgent need to devise management approaches and new technologies to reduce atmospheric greenhouse gas concentrations (GHG) in the near term. However, each year that GHG concentrations continue to rise, pressure mounts to develop and deploy atmospheric CO 2 removal pathways as a complement to, and not replacement for, emissions reductions. Soil carbon sequestration (SCS) practices in working lands provide a low-tech and cost-effective means for removing CO 2 from the atmosphere while also delivering co-benefits to people and ecosystems. Our model estimates suggest that, assuming additive effects, the technical potential of combined SCS practices can provide 30%-70% of the carbon removal required by the Paris Climate Agreement if applied to 25%-50% of the available global land area, respectively. Atmospheric CO 2 drawdown via SCS has the potential to last decades to centuries, although more research is needed to determine the long-term viability at scale and the durability of the carbon stored. Regardless of these research needs, we argue that SCS can at least serve as a bridging technology, reducing atmospheric CO 2 in the short term while energy and transportation systems adapt to a low-C economy. Soil C sequestration in working lands holds promise as a climate change mitigation tool, but the current rate of implementation remains too slow to make significant progress toward global emissions goals by 2050. Outreach and education, methodology development for C offset registries, improved access to materials and supplies, and improved research networks are needed to accelerate the rate of SCS practice implementation. Herein, we present an argument for the immediate adoption of SCS practices in working lands and recommendations for improved implementation., (© 2023 John Wiley & Sons Ltd.)

Conventional methods of agriculture, especially tillage, are often accompanied by soil degradation in the form of erosion and organic matter depletion. Regenerative agricultural methods seek to repair soil ecosystems by building topsoil and soil organic matter (SOM), decreasing reliance on chemical fertilizers and increasing both water retention capacity and the diversity and quantity of soil microbial and fungal communities. The University of Washington (UW) student farm is an organic and regeneratively managed site on the UW Seattle campus. Over the past 20 years the farm gradually expanded so locations on the farm encompass both unimproved topsoil and soils managed regeneratively for periods of 5 to 20 years. This arrangement allows a time-trend analysis of soil development under regenerative methods. Measurements of topsoil thickness (defined as the distance from the ground surface to the base of the soil A horizon) and organic matter content were collected across 14 distinct plots on the farm to quantify trends over time and estimate net change in SOM (and soil organic carbon, or SOC). While SOM content weakly increased by 0.5% per year, topsoil thickness exhibited a significant linear increase of 0.86 cm per year. Over a twenty-year period under the management practices of the UW Farm total organic carbon storage in soils, determined using topsoil thickness, density, and SOC content, increased by between 4 and 14 t ha -1 yr -1 . The general increases in topsoil thickness, SOM content, and total soil carbon demonstrate the potential of soil-health-focused practices to help maintain a productive and efficient urban growing space., Competing Interests: The authors declare they have no competing interests., (©2023 Macray and Montgomery.)

Reason for Doing the Work: Plant biomass is a commonly used metric to assess agricultural health and productivity. Removing plant material is the most accurate method to estimate plant biomass, but this approach is time consuming, labor intensive, and destructive. Previous attempts to use indirect methods to estimate plant biomass have been limited in breadth and/or have added complexity in data collection and/or modeling. A cost-effective, quick, accurate, and easy to use and understand approach is desirable for use by scientists and growers., Objectives: An indirect method for estimating plant biomass using a drop-plate meter was explored for use in broad array of crop systems., Methods: Drop-plate data collected by more than 20 individuals from 16 crop types on 312 farms across 15 states were used to generate models to estimate plant biomass among and within crop types., Results: A linear model using data from all crop types explained approximately 67% of the variation in plant biomass overall. This model performed differently among crop types and stand heights, which was owed to differences among sample sizes and farming between annual and perennial systems. Comparatively, the model using the combined dataset explained more variance in biomass than models generated with commodity specific data, with the exception of wheat., Conclusions: The drop-plate approach described here was inexpensive, quick, simple, and easy to interpret, and the model generated was robust to error and accurate across multiple crop types. The methods met all expectations for a broad-use approach to estimating plant biomass and are recommended for use across all agroecosystems included in this study. While it may be useful in crops beyond those included, validation is suggested before application., Competing Interests: All authors are employed by the Ecdysis Foundation., (© 2023 Robertson et al.)

Cell-cultured protein technology has become increasingly attractive due to its sustainability and climate benefits. The aim of this study is to determine the nutritional quality of the human-induced pluripotent stem cell (hiPSC)-cultured proteins in an advanced 3D peptide hydrogel system for the highly efficient production of cell-cultured proteins. Our previous study demonstrated a PGmatrix peptide hydrogel for the 3D embedded culture of long-term hiPSC maintenance and expansion (PGmatrix-hiPSC (PG-3D)), which showed significantly superior pluripotency when compared with traditional 2D cell culture on Matrigel and/or Vitronectin and other existing 3D scaffolding systems such as Polyethylene glycol (PEG)-based hydrogels. In this study, we designed a PGmatrix 3D suspension (PG-3DSUSP) system from the PG-3D embedded system that allows scaling up a hiPSC 3D culture volume by 20 times (e.g., from 0.5 mL to 10 mL). The results indicated that the PG-3DSUSP was a competitive system compared to the well-established PG-3D embedded method in terms of cell growth performance and cell pluripotency. hiPSCs cultured in PG-3DSUSP consistently presented a 15-20-fold increase in growth and a 95-99% increase in viability across multiple passages with spheroids with a size range of 30-50 μm. The expression of pluripotency-related genes, including NANOG , OCT4 , hTERT , REX1 , and UTF1 , in PG-3DSUSP-cultured hiPSCs was similar to or higher than that observed in a PG-3D system, suggesting continuous pluripotent maintenance. The nutritional value of the hiPSC-generated proteins from the PG-3DSUSP system was further evaluated for amino acid composition and in vitro protein digestibility. The amino acid composition of the hiPSC-generated proteins demonstrated a significantly higher essential amino acid content (39.0%) than human skeletal muscle protein (31.8%). In vitro protein digestibility of hiPSC-generated proteins was significantly higher (78.0 ± 0.7%) than that of the commercial beef protein isolate (75.7 ± 0.6%). Taken together, this is the first study to report an advanced PG-3DSUSP culture system to produce highly efficient hiPSC-generated proteins that possess more essential amino acids and better digestibility. The hiPSC-generated proteins with superior nutrition quality may be of particular significance as novel alternative proteins in food engineering and industries for future food, beverage, and supplement applications.

Sustainability of agricultural production and mitigation of global warming rely on the regeneration of soil organic carbon (SOC), in particulate organic carbon (POC) and mineral-associated organic carbon (MAOC) forms. We conducted a global systematic meta-analysis of the effects of regenerative management practices on SOC, POC, and MAOC in cropland, finding: 1) no-till (NT) and cropping system intensification increase SOC (11.3% and 12.4%, respectively), MAOC (8.5% and 7.1%, respectively), and POC (19.7% and 33.3%, respectively) in topsoil (0 to 20 cm), but not in subsoil (>20 cm); 2) experimental duration, tillage frequency, the intensification type, and rotation diversity moderate the effects of regenerative management; and 3) NT synergized with integrated crop-livestock (ICL) systems to greatly increase POC (38.1%) and cropping intensification synergized with ICL systems to greatly increase MAOC (33.1 to 53.6%). This analysis shows that regenerative agriculture is a key strategy to reduce the soil C deficit inherent to agriculture to promote both soil health and long-term C stabilization.

Introduction: Use of chemical fertilization and pesticides not only harm the environment but also have detrimental consequences on human health. In recent years, there has been a major emphasis worldwide on natural agriculture methods. Regenerative agriculture is known across the world as a combination of nature-friendly farming practices such as no-till, cover cropping, crop-rotation, agroforestry and use of organic home-based/farm-based ingredients to revive soil health. In India, a number of farmers are slowly adopting these practices using home-based mixtures and farmyard manure for soil rejuvenation and pest management. In order to evaluate the efficacy of the regenerative agriculture practices, this study compared conventional and regenerative agriculture plots for their soil bacterial and nutrient profiles., Methods: Two crops - ragi (Finger millet, an old world cereal eaten in India) and vegetable (tomato/beans), and different lengths (≤3 and >5 years) of regenerative practices were additional metrics considered to understand variabilities due to crop-type and period of application. The common regenerative agriculture practices used by farmers in this study included a mix of practices such as mulching, minimal-till, inter-cropping, crop-rotation, along with application of farmyard manure and other home-based concoctions rich in nutrients and microbes for enriching the soil., Results: We found that all regenerative practices were effective in bringing about an enrichment for soil bacteria with a more heterogeneous composition. Additionally, in regenerative vegetable (RV) versus conventional vegetable (CV) and barren land (BL) plots the relative percentage abundance of Actinobacteriota (RV-7.47%/ CV-6.24%/BL -7.02%) and Chloroflexi (RV-9.37%/ CV-6.63%/BL-8.75%) was slightly higher. In contrast, levels of Acidobacteriota (RV-8.1%/ CV-9.88%/BL-9.62%) was significantly lower. Similarly, regenerative ragi (RR) in comparison with conventional ragi (CR) and barren land (BL) plots saw higher representation of Firmicutes (RR-5.45%/ CR-2.38%/BL-1.45%) and Actinobacteriota (RR-11.53%/ CR-7.08%/BL-7.15%) and a concurrent reduction in Acidobacteriota (RR-6.91%/CR-7.39%/ BL-9.79%). The RV plots were found to be enriched for Plant Growth Promoting Rhizobacteria (PGPRs) - Pseudomonas sp. (RV-0.51%/CV-0.01%/BL-0.21%), and RR plots were enriched for Bacillus sp. (RR-1.35%/CR-0.95%/BL-0.61%), and Mesorhizobium sp. (0.30%/0.12%/0.21%), which are known to play significant roles in vegetable and ragi growth respectively., Discussion: Interestingly, long-term regenerative agriculture was able to support good nutrient composition while enhancing Soil Organic Carbon (SOC) levels. In all, the regenerative agriculture practices were found to be effective in improving bacterial community structure and simultaneously improving soil health. We found that BL soil with eucalyptus plantation showed among the least bacterial diversity suggesting detrimental impact on soil health., Competing Interests: Conflict of interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

European agri-environment schemes include the use of flower-rich field margins to promote on-farm biodiversity, but species mixtures rarely include Brassicaceae. As pests of oilseed rape (OSR; Brassica napus ) and their parasitoids are mostly brassica specialists, including brassica 'banker plants' in the mixtures would help support these important biocontrol agents and improve pest control throughout the crop rotation. We assessed the potential of six brassicaceous plants (replicated plots grown in the field) to enhance populations of parasitoids of OSR pests whilst minimising proliferation of their pest hosts. Fodder radish ( Raphanus sativus ) facilitated high production of parasitoids of the pollen beetle pest ( Brassicogethes aeneus ) but may proliferate Ceutorhynchus weevil pests due to low parasitism. Turnip rape ( B. rapa ) and the B. rapa hybrid 'Tyfon' showed potential to perform a trap cropping function for pests, but their early flowering phenology resulted in B. aeneus larvae escaping parasitisation, potentially assisting proliferation of this pest. Forage rape B. napus exhibited similarly high B. aeneus parasitoid production characteristics to R. sativus but did not potentiate problems with other pests, indicating that it would be a favourable banker plant option. Careful selection of plants in field margin mixtures is therefore needed to maximise their benefits and ideally the whole crop pest-beneficial complex needs to be studied, as focus on a single major pest risks unintended consequences with other pest problems.

Managing soils to retain new plant inputs is key to moving toward a sustainable and regenerative agriculture. Management practices, like diversifying and perennializing agroecosystems, may affect the decomposer organisms that regulate how new residue is converted to persistent soil organic matter. Here we tested whether 12 years of diversifying/perennializing plants in agroecosystems through extended rotations or grassland restoration would decrease losses of new plant residue inputs and, thus, increase retention of carbon (C) and nitrogen (N) in soil. We tracked dual-labeled ( 13 C and 15 N), isotopically enriched wheat (Triticum aestivum) residue in situ for 2 years as it decomposed in three agroecosystems: maize-soybean (CS) rotation, maize-soybean-wheat plus red clover and cereal rye cover crops (CSW2), and spring fallow management with regeneration of natural grassland species (seven to 10 species; SF). We measured losses of wheat residue (C wheat and N wheat ) in leached soil solution and greenhouse gas fluxes, as well as how much was recovered in microbial biomass and bulk soil at 5-cm increments down to 20 cm. CSW2 and SF both had unique, significant effects on residue decomposition and retention dynamics that were clear only when using nuanced metrics that able to tease apart subtle differences. For example, SF retained a greater portion of C wheat in 0-5 cm surface soils (155%, p = 0.035) and narrowed the C wheat to N wheat ratio (p < 0.030) compared to CS. CSW2 increased an index of carbon-retention efficiency, C wheat retained in the mesocosm divided by total measured, from 0.18 to 0.27 (49%, p = 0.001), compared to CS. Overall, we found that diversifying and extending the duration of living plants in agroecosystems can lead to greater retention of new residue inputs in subtle ways that require further investigation to fully understand., (© 2022 The Authors. Ecological Applications published by Wiley Periodicals LLC on behalf of The Ecological Society of America.)

Cover crops have been studied for over a century, but the recognition of a complex interaction of cover crop on the Earth's biosphere, lithosphere, hydrosphere, and atmosphere is relatively recent. Furthermore, previously published cover crop research has largely focused on evaluating cover crop impacts on subsequent crop yield. Understanding the cover crop-induced benefits on soil organic carbon (SOC) sequestration, nitrous oxide (N 2 O) emissions, wind and water erosion, weed control, and soil microbial communities has gained considerable attention in the last few decades, which is crucial to make progress towards developing sustainable agricultural production systems. New research is continuously published to gain a comprehensive understanding of the multiple ecosystem services provided by cover crops. Here, in this review, we aimed to (a) summarize current knowledge related to cover crop impacts on agroecosystem functioning and explore the potential mechanisms responsible for those effects, and (b) identify the key factors limiting the adoption of cover crops into agroecosystems and the conspicuous knowledge gaps in cover crop research. Overall, the review results suggest that cover crops increased subsequent crop yield, increased SOC storage, increased weed suppression, mitigated N 2 O emissions, reduced wind and water erosion, suppressed plant pathogens, and increased soil microbial activity and wildlife biodiversity. However, the magnitude of benefits observed with cover crops varied with cover crop type, location, and the duration of cover cropping. Notably, cover crop termination methods, designing crop rotations to fit cover crops, additional costs associated with cover crop integration, and uncertainty related to economic returns with cover crops are some of the major barriers limiting the adoption of cover crops into production systems, particularly in North America. In addition to long-term effects, future research on cover crop agronomy, breeding cover crop cultivars, and interactive effects of cover crops with other sustainable land management practices is needed., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)

The article presents an overview of research conducted in recent years, i.e., from 2004 until now. The study has been prompted by the threat of drought over large land areas which, as a result of current climate change, may lead to desertification in dry and hot regions of the world. For the same reason, large areas of farmland are affected by drought stress. At the same time, rising air temperatures result in a significant intensification of evaporation and a gradual increase in soil salinity. This applies in particular to acres of farmland, forested areas, and green areas of cities, as well as degraded land or brownfields. As the crop stability is threatened, the food base of the world's population is at risk and, additionally, in areas of industrial districts, people's health is in decline. Due to these multistress conditions for plant growth, we propose a review of the current literature which addresses the possibility of counteracting these unfavorable phenomena through the appropriate selection of plant species and, when only applicable, also through specific agroecological treatments. A selection of herbaceous and woody plants useful for cultivation on saline marginal lands was proposed.

Conventional arable cropping with annual crops established by ploughing and harrowing degrades larger soil aggregates that contribute to storing soil organic carbon (SOC). The urgent need to increase SOC content of arable soils to improve their functioning and sequester atmospheric CO 2 has motivated studies into the effects of reintroducing leys into long-term conventional arable fields. However, effects of short-term leys on total SOC accumulation have been equivocal. As soil aggregation may be important for carbon storage, we investigated the effects of arable-to-ley conversion on cambisol soil after three years of ley, on concentrations and stocks of SOC, nitrogen and their distributions in different sized water-stable aggregates. These values were benchmarked against soil from beneath hedgerow margins. SOC stocks (0-7 cm depth) rose from 20.3 to 22.6 Mg ha -1 in the arable-to-ley conversion, compared to 30 Mg ha -1 in hedgerows, but this 2.3 Mg ha -1 difference (or 0.77 Mg C ha -1 yr -1 ) was not significant). However, the proportion of large macroaggregates (> 2000 μm) increased 5.4-fold in the arable-to-ley conversion, recovering to similar abundance as hedgerow soils, driving near parallel increases in SOC and nitrogen within large macroaggregates (5.1 and 5.7-fold respectively). The total SOC (0-7 cm depth) stored in large macroaggregates increased from 2.0 to 9.6 Mg ha -1 in the arable-to-ley conversion, which no longer differed significantly from the 12.1 Mg ha -1 under hedgerows. The carbon therefore accumulated three times faster, at 2.53 Mg C ha -1 yr -1 , in the large macroaggregates compared to the bulk soil. These findings highlight the value of monitoring large macroaggregate-bound SOC as a key early indicator of shifts in soil quality in response to change in field management, and the benefits of leys in soil aggregation, carbon accumulation, and soil functioning, providing justification for fiscal incentives that encourage wider use of leys in arable rotations., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)

The soil microbiome contributes to several ecosystem processes. It plays a key role in sustainable agriculture, horticulture and forestry. In contrast to the vast number of studies focusing on soil bacteria, the amount of research concerning soil fungal communities is limited. This is despite the fact that fungi play a crucial role in the cycling of matter and energy on Earth. Fungi constitute a significant part of the pathobiome of plants. Moreover, many of them are indispensable to plant health. This group includes mycorrhizal fungi, superparasites of pathogens, and generalists; they stabilize the soil mycobiome and play a key role in biogeochemical cycles. Several fungal species also contribute to soil bioremediation through their uptake of high amounts of contaminants from the environment. Moreover, fungal mycelia stretch below the ground like blood vessels in the human body, transferring water and nutrients to and from various plants. Recent advances in high-throughput sequencing combined with bioinformatic tools have facilitated detailed studies of the soil mycobiome. This review discusses the beneficial effects of soil mycobiomes and their interactions with other microbes and hosts in both healthy and unhealthy ecosystems. It may be argued that studying the soil mycobiome in such a fashion is an essential step in promoting sustainable and regenerative agriculture., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Frąc, Hannula, Bełka, Salles and Jedryczka.)

The development of modern, industrial agriculture and its high input-high output carbon energy model is rendering agricultural landscapes less resilient. The expected continued increase in the frequency and intensity of extreme weather events, in conjunction with declining soil health and biodiversity losses, could make food more expensive to produce. The United Nations has called for global action by establishing 17 sustainable development goals (SDGs), four of which are linked to food production and security: declining biodiversity (SDG 15), loss of ecosystem services and agroecosystem stability caused by increasing stress from food production intensification and climate change (SDG 13), declining soil health caused by agricultural practices (SDGs 2 and 6), and dependence on synthetic fertilizers and pesticides to maintain high productivity (SDG 2). To achieve these SDGs, the agriculture sector must take a leading role in reversing the many negative environmental trends apparent in today's agricultural landscapes to ensure that they will adapt and be resilient to climate change in 2030 and beyond. This will demand fundamental changes in how we practice agriculture from an environmental standpoint. Here, we present a perspective focused on the implementation of an agrosystem approach, which we define to promote regenerative agriculture, an integrative approach that provides greater resilience to a changing climate, reverses biodiversity loss, and improves soil health; honors Indigenous ways of knowing and a holistic approach to living off and learning from the land; and supports the establishment of emerging circular economies and community well-being. Integr Environ Assess Manag 2022;18:1199-1205. © 2021 SETAC., (© 2021 SETAC.)

Wheat yields have plateaued in the UK over the last 25 years, during which time most arable land has been annually cropped continuously with short rotations dominated by cereals. Arable intensification has depleted soil organic matter and biology, including mycorrhizas, which are affected by tillage, herbicides, and crop genotype. Here, we test whether winter wheat yields, mycorrhization, and shoot health can be improved simply by adopting less intensive tillage and adding commercial mycorrhizal inoculum to long-term arable fields, or if 3-year grass-clover leys followed direct drilling is more effective for biological regeneration of soil with reduced N fertiliser. We report a trial of mycorrhization, ear pathology, and yield performance of the parents and four double haploid lines from the Avalon x Cadenza winter wheat population in a long-term arable field that is divided into replicated treatment plots. These plots comprised wheat lines grown using ploughing or disc cultivation for 3 years, half of which received annual additions of commercial arbuscular mycorrhizal (AM) inoculum, compared to 3-year mown grass-clover ley plots treated with glyphosate and direct-drilled. All plots annually received 35 kg of N ha -1 fertiliser without fungicides. The wheat lines did not differ in mycorrhization, which averaged only 34% and 40% of root length colonised (RLC) in the ploughed and disc-cultivated plots, respectively, and decreased with inoculation. In the ley, RLC increased to 52%. Two wheat lines were very susceptible to a sooty ear mould, which was lowest in the ley, and highest with disc cultivation. AM inoculation reduced ear infections by >50% in the susceptible lines. In the ley, yields ranged from 7.2 to 8.3 t ha -1 , achieving 92 to 106% of UK average wheat yield in 2018 (7.8 t ha -1 ) but using only 25% of average N fertiliser. Yields with ploughing and disc cultivation averaged only 3.9 and 3.4 t ha -1 , respectively, with AM inoculum reducing yields from 4.3 to 3.5 t ha -1 in ploughed plots, with no effect of disc cultivation. The findings reveal multiple benefits of reintegrating legume-rich leys into arable rotations as part of a strategy to regenerate soil quality and wheat crop health, reduce dependence on nitrogen fertilisers, enhance mycorrhization, and achieve good yields., Competing Interests: Author RS was employed by RAGT Seeds Ltd. Author DB was employed by ADAS Gleadthorpe, RSK Group. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Austen, Tille, Berdeni, Firbank, Lappage, Nelson, Helgason, Marshall-Harries, Hughes, Summers, Cameron and Leake.)

Increased tolerance to competition for soil resources of modern maize ( Zea mays L.) hybrids increases soil resource use efficiency and yield. Yet little information is available on the relationship between maize population density and yield under no-tillage in semi-arid environments. A 2-year field trial was conducted in South Africa during the 2017/2018 (Season 1) and 2018/2019 (Season 2) production seasons to evaluate growth and water use productivity of rainfed maize established at seven diverse plant population (20,000-60,000 plants ha -1 ) and row spacing (0.52 and 0.76 m) configurations. In Season 1, light interception was 6.8% greater at 0.76 m row spacing compared to 0.52 m row spacing ( p  < 0.05). In Season 2, despite dry and hot growing conditions, a well-developed leaf canopy cover was present at 0.52 m row spacing indicating a 10.4% greater intercepted photosynthetically active radiation (IPAR) compared to 0.76 m row spacing. In Season 1, with more uniform rainfall distribution, no biomass or yield benefits were found with increased plant population, except at 50,000 plants ha -1 at 0.76 m row spacing. In Season 2, plant populations at 0.76 m row spacing out-yielded any given plant population at 0.52 m row spacing. The optimal plant population and row spacing will ultimately be a compromise between obtaining high maize grain yield and minimizing the potential for crop failure in semi-arid environments., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Haarhoff and Swanepoel.)

Ergothioneine (ERGO) is a potent antioxidant and anti-inflammatory amino acid that is highly bioavailable to humans from the diet. ERGO is now regarded by some as a 'longevity vitamin' that has the potential to mitigate some chronic diseases of ageing and thereby increase life expectancy when present in adequate amounts. However, only limited knowledge exists regarding ERGO content in the human diet. Since ERGO is produced primarily by fungi, mushrooms are known to be the leading dietary source, but ERGO is found in relatively low amounts throughout the food chain as a result of soil-borne fungi or bacteria passing it on to plants through their roots. Some conventional agricultural practices that negatively impact soil fungi, such as excessive soil disturbance (ploughing), can significantly reduce ERGO content of food crops when compared to regenerative practices such as eliminating tillage of the soil (no-till). This has led us to the concept that ERGO may be a definitive connection between soil health and human health., (© 2021 Federation of European Biochemical Societies.)

In semi-arid regions, post-restoration vegetation recovery on abandoned agricultural lands often fails due to inherently low organic matter content and poor soil fertility conditions, including phosphorus (P). As such, amending these soils with controlled release P fertilizer, especially with suitable P solubilizing bacteria (PSB) may promote plant growth and productivity by stimulating biological P fertility. To this aim, a pot study was performed to evaluate the agronomic potential of maize and soil biological P pools, using encapsulated (ENRP) and non-encapsulated (NRP) nano-rock phosphate as the P fertilizer source, on reclaimed agricultural soil in the presence and absence of PSB inoculant. The experiment was setup following a 3 × 2 factorial arrangement with four replicates. Without PSB, NRP treatment showed marginal positive effects on plant growth, P nutrition and P use efficiency (PUE) compared to control treatment. Although larger gains with NRP treatment were more noticeable under PSB inoculation, ENRP was the most convenient slow-release P fertilizer, increasing plant growth, P nutrition and grain yield compared to all treatments. Importantly, PSB inoculation with ENRP resulted in significantly higher increase in soil CaCl 2 -P (8.91 mg P kg soil -1 ), citrate-P (26.98 mg P kg soil -1 ), enzyme-P (18.98 mg P kg soil -1 ), resin-P (11.41 mg P kg soil -1 ), and microbial-P (18.94 mg P kg soil -1 ), when compared to all treatment combinations. Although a decrease in soil HCl-P content was observed with both types of P fertilizer, significant differences were found only with PSB inoculation. A significant increase in soil biological P pools could be due to the higher specific area and crystalline structure of nano materials, providing increased number of active sites for PSB activity in the presence of biobased encapsulated shell. Furthermore, the increase in PSB abundance, higher root carboxylate secretions, and decreased rhizosphere pH in response to nano-structured P fertilizer, implies greater extension of rhizosphere promoting greater P mobilization and/or solubilization, particularly under PSB inoculated conditions. We conclude that cropping potential of abandoned agricultural lands can be enhanced by the use of nano-rock phosphate in combination with PSB inoculant, establishing a favorable micro-environment for higher plant growth and biochemical P fertility., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)

Several independent comparisons indicate regenerative farming practices enhance the nutritional profiles of crops and livestock. Measurements from paired farms across the United States indicate differences in soil health and crop nutrient density between fields worked with conventional (synthetically-fertilized and herbicide-treated) or regenerative practices for 5 to 10 years. Specifically, regenerative farms that combined no-till, cover crops, and diverse rotations-a system known as Conservation Agriculture-produced crops with higher soil organic matter levels, soil health scores, and levels of certain vitamins, minerals, and phytochemicals. In addition, crops from two regenerative no-till vegetable farms, one in California and the other in Connecticut, had higher levels of phytochemicals than values reported previously from New York supermarkets. Moreover, a comparison of wheat from adjacent regenerative and conventional no-till fields in northern Oregon found a higher density of mineral micronutrients in the regenerative crop. Finally, a comparison of the unsaturated fatty acid profile of beef and pork raised on one of the regenerative farms to a regional health-promoting brand and conventional meat from local supermarkets, found higher levels of omega-3 fats and a more health-beneficial ratio of omega-6 to omega-3 fats. Despite small sample sizes, all three crop comparisons show differences in micronutrient and phytochemical concentrations that suggest soil health is an under appreciated influence on nutrient density, particularly for phytochemicals not conventionally considered nutrients but nonetheless relevant to chronic disease prevention. Likewise, regenerative grazing practices produced meat with a better fatty acid profile than conventional and regional health-promoting brands. Together these comparisons offer preliminary support for the conclusion that regenerative soil-building farming practices can enhance the nutritional profile of conventionally grown plant and animal foods., Competing Interests: Anne Biklé is a principal author at Dig2Grow.com. Paul Brown and Jazmin Jordan work at Brown’s Ranch, a regenerative farm sampled in the study., (© 2022 Montgomery et al.)

In recent years, interest has increased in regenerative practices as a strategy for transforming food systems and solving major environmental problems such as biodiversity loss and climate change. However, debates persist regarding these practices and how they ought to be defined. This paper presents a framework for exploring the regenerative potential of food systems, focusing on how food systems activities and technologies are organized rather than the specific technologies or practices being employed. The paper begins with a brief review of debates over sustainable food systems and the varying ways that regenerative food systems have been defined and theorized. Then, it provides the theoretical backing of the framework-the conservation of change principle-which is an interpretation of the laws of thermodynamics and theories of adaptive change as relevant to the regenerative capacity of living systems. Next, the paper introduces the framework itself, which comprises two independent but intersecting dimensions of food systems organization: resource diversity and livelihood flexibility. These two dimensions result in four archetypical regimes for food systems: degenerative, regenerative, impoverished, and coerced. The paper defines each and offers real-world examples. Finally, the paper concludes with a discussion of pathways for transforming food systems and opportunities for additional research., (© The Author(s), under exclusive licence to Springer Nature B.V. 2021.)

Agriculture occupies 38% of the planet's terrestrial surface, using 70% of freshwater resources. Its modern practice is dominated by an industrial-productivist discourse, which has contributed to the simplification and degradation of human and ecological systems. As such, agricultural transformation is essential for creating more sustainable food systems. This paper focuses on discursive change. A prominent discursive alternative to industrial-productivist agriculture is regenerative agriculture. Regenerative discourses are emergent, radically evolving and diverse. It is unclear whether they have the potential to generate the changes required to shift industrial-productivist agriculture. This paper presents a literature-based discourse analysis to illustrate key thematic characteristics of regenerative agricultural discourses. The analysis finds that such discourses: situate agricultural work within nested, complex living systems; position farms as relational, characterised by co-evolution between humans and other landscape biota; perceive the innate potential of living systems as place-sourced; maintain a transformative openness to alternative thinking and practice; believe that multiple regenerative cultures are necessary for deeply regenerative agriculture; and depart from industrialism to varying degrees. The paper concludes by reviewing three transformative opportunities for regenerative discourses-discourse coalitions, translocal organising and collective learning., Supplementary Information: The online version supplementary material available at 10.1007/s10460-021-10276-0., (© Crown 2021.)

There is mounting evidence for the potential for the natural dietary antioxidant and anti-inflammatory amino acid l-Ergothioneine (ERGO) to prevent or mitigate chronic diseases of aging. This has led to the suggestion that it could be considered a 'longevity vitamin.' ERGO is produced in nature only by certain fungi and a few other microbes. Mushrooms are, by far, the leading dietary source of ERGO, but it is found in small amounts throughout the food chain, most likely due to soil-borne fungi passing it on to plants. Because some common agricultural practices can disrupt beneficial fungus-plant root relationships, ERGO levels in foods grown under those conditions could be compromised. Thus, research is needed to further analyse the role agricultural practices play in the availability of ERGO in the human diet and its potential to improve our long-term health., (© The Author(s) 2020.)

Background: Organic viticulture can generate a range of ecosystem services including supporting biodiversity, reducing the use of conventional pesticides and fertilizers, and mitigating greenhouse gas emissions through long-term carbon (C) storage. Here we focused on aboveground C storage rates and accumulation using a one-year increment analysis applied across different winegrape varietals and different-aged vineyard blocks. This produced a chronosequence of C storage rates over what is roughly the productive lifespan of most vines (aged 2-30 years). To our knowledge, this study provides the first estimate of C storage rates in the woody biomass of vines. Additionally, we assessed C storage in wildland buffers and adjacent oak-dominated habitats over a 9-year period., Results: Carbon storage averaged 6.5 Mg/Ha in vines. We found the average annual increase in woody C storage was 43% by mass. Variation correlated most strongly with vine age, where the younger the vine, the greater the relative increase in annual C. Decreases in C increment rates with vine age were more than offset by the greater overall biomass of older vines, such that C on the landscape continued to increase over the life of the vines at 18.5% per year on average. Varietal did not significantly affect storage rates or total C stored. Carbon storage averaged 81.7 Mg/Ha in native perennial buffer vegetation; we found an 11% increase in mass over 9 years for oak woodlands and savannas., Conclusions: Despite a decrease in the annual rate of C accumulation as vines age, we found a net increase in aboveground C in the woody biomass of vines. The results indicate the positive role that older vines play in on-farm (vineyard) C and overall aboveground accumulation rates. Additionally, we found that the conservation of native perennial vegetation as vineyard buffers and edge habitats contributes substantially to overall C stores. We recommend that future research consider longer time horizons for increment analysis, as this should improve the precision of C accumulation rate estimates, including in belowground (i.e., soil) reservoirs.

'Managed grazing' is gaining attention for its potential to contribute to climate change mitigation by reducing bare ground and promoting perennialization, thereby enhancing soil carbon sequestration (SCS). Understanding why ranchers adopt managed grazing is key to developing the right incentives. In this paper, we explore principles and practices associated with the larger enterprise of 'regenerative ranching' (RR), which includes managed grazing but infuses the practice with holistic decision-making. We argue that this broader approach is appealing due to a suite of ecological, economic and social benefits, making climate change mitigation an afterthought, or 'co-benefit'. RR is challenging, however, because it requires a deep understanding of ecological processes along with a set of skills related to monitoring and moving livestock and feeding the soil microbiome. We review the literature regarding links between RR and SCS, then present results of qualitative research focused on motivators, enablers and constraints associated with RR, drawing on interviews with 52 practitioners in New South Wales, Australia and the western United States. Our analysis is guided by a conceptual model of the social-ecological system associated with RR that identifies determinants of regenerative potential. We discuss implications for rancher engagement and conclude with a consideration of leverage points for global scalability., Competing Interests: We declare we have no competing interests., (© 2020 The Authors.)

The current industrialized food system that is the predominant model of production in the United States and increasingly in other regions of the world has been recognized as a major contributor to greenhouse gas emissions, public health crises, environmental and soil health degradation, animal welfare abuses, labor crises and more. This article describes the evolution and design of food systems over time and the current assessment that a new system is required that focuses on planetary health and the health and well-being of humans and animals. The article describes the growth in entrepreneurship in the food system today and concludes that innovation is in need of increased support in order to advance a new operating system for how we grow, produce and distribute food., (Copyright © 2020 The Author. Published by Elsevier Inc. All rights reserved.)

Regenerative agriculture is being used by a small number of innovative farmers in Australia and elsewhere, using a range of holistic methods that work with the land and climate, such as short duration time of controlled grazing with long rest periods for the paddock and higher proportions of aboveground biomass, to improve soil health and farm profitability. This paper uses a delta life cycle assessment, focusing only on the differences between regenerative and conventional production systems to assess the potential impact of regenerative agriculture on a full range of midpoint impact categories and end-point areas of protection for an extensive sheep system in Australia. We assess the potential improvement to the water, carbon, and biodiversity footprints of sheep production, and find that regenerative agriculture has the potential to improve environmental performance compared with current industrial agricultural practices. In particular, there seems to be considerable potential to offset a significant proportion of the on-farm climate change impacts through a combination of biosequestration in soils and aboveground biomass and using harvested biomass to offset fossil fuel use. The assessment highlights the need for additional data to confirm the findings and the potential contribution that regenerative agriculture can make to sustainability of ruminant livestock production. Integr Environ Assess Manag 2020;16:282-290. © 2019 SETAC., (© 2019 SETAC.)

Large-scale food system practices have diminished soil and water quality and negatively impacted climate change. Yet, numerous opportunities exist to harness food system practices that will ensure better outcomes for human health and ecosystems. The objective of this study was to consider food Production, Processing, Access and Consumption domains, and for each determine the challenges and successes associated with progressing towards a sustainable food system. A workshop engaging 122 participants including producers, consultants, consumers, educators, funders, scientists, media, government and industry representatives, was conducted in Perth, Western Australia. A thematic analysis of statements (Successes ( n = 170) or Challenges ( n = 360)) captured, revealed issues of scale, knowledge and education, economics, consumerism, big food, environmental/sustainability, communication, policies and legislation, and technology and innovations. Policy recommendations included greater investment into research in sustainable agriculture (particularly the evidentiary basis for regenerative agriculture), land preservation, and supporting farmers to overcome high infrastructure costs and absorb labour costs. Policy, practice and research recommendations included focusing on an integrated food systems approach with multiple goals, food system actors working collaboratively to reduce challenges and undertaking more research to further the regenerative agriculture evidence.

For addressing potential food shortages, a fundamental tradeoff exists between investing more resources to increasing productivity of existing crops, as opposed to increasing crop diversity by incorporating more species. We explore ways to use local plants as food resources and the potential to promote food diversity and agricultural resilience. We discuss how use of local plants and the practice of local agriculture can contribute to ongoing adaptability in times of global change. Most food crops are now produced, transported, and consumed long distances from their homelands of origin. At the same time, research and practices are directed primarily at improving the productivity of a small number of existing crops that form the cornerstone of a global food economy, rather than to increasing crop diversity. The result is a loss of agro-biodiversity, leading to a food industry that is more susceptible to abiotic and biotic stressors, and more at risk of catastrophic losses. Humans cultivate only about 150 of an estimated 30,000 edible plant species worldwide, with only 30 plant species comprising the vast majority of our diets. To some extent, these practices explain the food disparity among human populations, where nearly 1 billion people suffer insufficient nutrition and 2 billion people are obese or overweight. Commercial uses of new crops and wild plants of local origin have the potential to diversify global food production and better enable local adaptation to the diverse environments humans inhabit. We discuss the advantages, obstacles, and risks of using local plants. We also describe a case study-the missed opportunity to produce pine nuts commercially in the Western United States. We discuss the potential consequences of using local pine nuts rather than importing them overseas. Finally, we provide a list of edible native plants, and synthesize the state of research concerning the potential and challenges in using them for food production. The goal of our synthesis is to support more local food production using native plants in an ecologically sustainable manner.