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Water provisioning is common in arid and semi-arid African ecosystems, but its effects on carnivore communities are not understood. Recently, Morin et al. documented the unexpectedly contrasting space-use patterns of dominant and subordinate carnivores around water sources, with dominant carnivores facilitating subordinate carnivores., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Herein, the recent advances in the development of resorbable polymeric-based biomaterials, their geometrical forms, resorption mechanisms, and their capabilities in various biomedical applications are critically reviewed. A comprehensive discussion of the engineering approaches for the fabrication of polymeric resorbable scaffolds for tissue engineering, drug delivery, surgical, cardiological, aesthetical, dental and cardiovascular applications, are also explained. Furthermore, to understand the internal structures of resorbable scaffolds, representative studies of their evaluation by medical imaging techniques, e.g., cardiac computer tomography, are succinctly highlighted. This approach provides crucial clinical insights which help to improve the materials' suitable and viable characteristics for them to meet the highly restrictive medical requirements. Finally, the aspects of the legal regulations and the associated challenges in translating research into desirable clinical and marketable materials of polymeric-based formulations, are presented., (© 2024 The Author(s). Advanced Healthcare Materials published by Wiley‐VCH GmbH.)

Natural fibers are being employed in the development of the next generation of biomaterials, due to their reduced environmental impact and the ease of their functionalization with natural polymers. In this study, Barba del Viejo fibers were treated with NaOH and bonded with alginate through ionic gelation by using CaCℓ 2 , which facilitates ionic interactions between alginate and Ca 2+ ions. Additionally, antimicrobial Barba del Viejo/alginate/silver nanoparticles(BVA/Ag o ) scaffolds were developed from the NaOH-treated BV-microfibers, silver salt and sodium alginate, with mint leaves extract as a nucleating agent. The functional and crystalline structure of microfibers and the hydrogels developed were analyzed using the FTIR and XRD. SEM explained that the diameter of the NaOH-treated microfibers was ⁓1.32 μm. The morphological images of the hydrogels, confirm the functionalization of alginate with microfibers and the formation of Ag o nanoparticles within the hydrogels network. The swelling ratio of the hydrogels increased with alginate functionalization on BV-microfibers, improving NaOH treatment, but decreased the degradation rate. UV-spectra showed absorption peaks in the wavelength range of between 432 and 442 nm, confirming the surface plasmon resonance effect of the Ag o nanoparticles within the scaffolds. TEM analysis confirms that the Ag o nanoparticles in hydrogels were spherical in shape, with sizes ranging from ⁓2 and 10 nm. The zeta potential analysis indicates that the Ag o nanoparticles possess negative charges, providing a stable surface that helps to prevent aggregation and, therefore, demonstrating a homogeneous distribution throughout all the BVA/Ag o scaffolds prepared. The antimicrobial studies reveal that the BVA/Ag o scaffolds exhibit significant antibacterial activity against E.coli and S.aureus bacteria. Further investigations are affirmed to study the potential applications of these scaffolds in infection-control wound dressing., Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Introduction: In South Africa, access to higher education is viewed as a pathway to improved life chances. However, the transition from school to university is a stressful experience for students, marked by significant challenges. Although literature acknowledges these challenges, limited research has focused specifically on the role of hope during this period. Additionally, previous studies on hope have often utilized individualistic approaches, which may not fully capture the experience of students in collectivist cultures. This study addresses this gap by exploring hope from a culturally sensitive perspective within a collectivist context, aiming to understand how first-year South African university students experience hope during the transition to university., Methods: This study employed Interpretative Phenomenological Analysis (IPA) to explore the lived experiences of hope among first-year South African university students during their transition to higher education. Twenty-two students participated in semi-structured interviews, allowing for an in-depth examination of their personal and cultural perspectives on hope and the challenges they faced. The data were analyzed iteratively, with strategies implemented to enhance trustworthiness and credibility, ensuring a thorough interpretation of students' experiences., Results: The analysis yielded three main themes: (1) Affective and Social Duality: Students described mixed emotions and social challenges as they entered university, highlighting the dual nature of their experiences in adapting to a new environment. (2) Hope as a Multifaceted Concept: Hope was portrayed as a guiding force that helped students navigate periods of uncertainty. Participants described hope not just as a single idea but as a complex, evolving concept crucial to their resilience. (3) Beyond Academic Aspirations: Hope extended beyond academic success and was closely tied to personal fulfilment and the desire to contribute positively to society. This broader perspective on hope suggests that students' aspirations were not confined to individual achievement but also included a sense of collective and societal impact., Discussion: The study reveals that hope is a multidimensional construct that significantly influences students' transitional experiences, extending beyond academic goals to include personal growth and societal contributions. This finding challenges the traditional, individualistic approaches to studying hope by highlighting the cultural relevance of collectivist values in shaping students' experiences. The study underscores the need for culturally sensitive research and suggests that student support services should consider cultural contexts to address students' unique challenges and aspirations better. Future research is recommended to explore hope across various cultural backgrounds to gain a more comprehensive understanding of its role in student development., Competing Interests: The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Mason.)

Gonadal hormones play a central role in reproductive function and success. As such, quantifying reproductive hormones non-invasively in threatened, vulnerable and endangered wildlife species offers an ideal tool for assessing general and individual reproductive patterns in situ . Whilst the use of faeces as a hormone matrix is often preferred in these cases, the required enzyme immunoassays (EIAs) for measuring faecal androgen (fAM), oestrogen (fEM) and progestagen metabolite (fPM) concentrations must first be validated if a species gets investigated for the first time to ensure biologically relevant patterns can be observed. In this study we aimed to biologically validate the EIAs for monitoring fAM, fEM and fPM concentrations in Temminck's pangolin, Smutsia temminckii . Hormone metabolite concentrations derived from each EIA tested were compared between different age and sex classes. An epiandrosterone EIA effectively measured androgen levels in males, distinguishing between adult and juvenile individuals, as well as both female age classes. Similarly, the tested oestrogen EIA successfully distinguished between adult and juvenile female fEM concentrations, and both tested progestagen EIAs demonstrated adequate differences between fPM concentrations of adult and juvenile females. The now-validated EIAs offer robust tools for a non-invasive monitoring of reproductive activity in Temminck's pangolin. The development of such techniques will allow researchers to assess reproductive hormone patterns of the species in situ , whilst also paving the way for further studies in this field. Despite the small sample size due to the species' conservation status, the study provides a foundation for future research using a robust, validated, non-invasive monitoring tool. The latter can now be implemented in long-term monitoring with larger sample sizes to yield more comprehensive data, aiding in the conservation of Temminck's pangolin., Competing Interests: The authors have no conflicts of interest to declare., (© The Author(s) 2024. Published by Oxford University Press and the Society for Experimental Biology.)

Eco-friendly antimicrobial bio-composite films (BCF) were produced by using guar gum (GG), flax seed mucilage (FM) and polyvinyl alcohol (PVA), supplemented with cell-free supernatant (CFS) of Lactobacillus plantarum (L. p) and Lactobacillus delbrueckii (L. d) by the solvent casting technique. The BCF was categorized into: PVA, PVA + FM, PVA + GG, PVA + FM + GG, PVA + FM + CFS, PVA + GG + CFS, PVA + FM + GG + CFS. The film's mechanical, morphological, physical, and antimicrobial properties were characterised. The mechanical, and hydrophobic features of the BCF were increased with the incorporation of FM, GG, and these attributes were faintly decreased with the fusion of postbiotics in films. However, BCF with postbiotics exhibited antimicrobial activity and UV barrier and biodegradable traits, while BCF without postbiotics did not show any antimicrobial effect. According to the characterization study the fabricated PVA + FM + GG and PVA + FM + GG + CFS was employed as packaging material for fig fruit to prolong the shelf life. Overall, PVA + FM + GG + CFS-based packed fig fruits has prolonged shelf life of till 12 days., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The Spiny saltbush ( Rhagodia spinscens ) is a halophyte species with the potential to provide natural ingredients used in food and pharmaceutical industries. In food and pharmaceutical applications, drying is necessary to maintain shelf-life, which reduces phytonutrient content. In this study, changes in the nutritional composition, phenolic and carotenoid profiles of radical antioxidant scavenging activity [(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)], antioxidant power [ferric reducing antioxidant ability assay (FRAP)], and cytotoxicity of freeze- and oven-dried (55 °C for 24 h) spiny saltbush were determined. Sodium (4.72 g/100 g dry weight (DW), potassium (6.86 g/100 g DW), calcium (4.06 g/100 g DW), zinc (372 mg/kg DW) and protein content were higher in oven-dried samples than freeze-dried samples. Ultra-performance liquid chromatography-mass spectrometry analysis detected 18 metabolites in saltbush extracts. Partial Least-Squares Discriminant Analysis, Hierarchical Cluster Analysis, and Variable Importance in Projection discriminated between freeze-dried and oven-dried samples. Freeze-dried samples retained more individual metabolites than oven-dried samples, while oven-dried samples had higher antioxidant activity (ABTS and FRAP), lutein, trans-β carotene, and cis-β-carotene. Correlation analysis identified potential antioxidant candidates between phenolic and carotenoid compounds. Neither freeze-dried nor oven-dried spiny saltbush samples showed cytotoxicity. The study uncovered changes in phytonutritional compounds after the oven and freeze-drying spiny saltbush, a potential salt alternative and functional ingredient for the food industry.

The progress of 3D instance segmentation techniques has made it essential for several applications, such as augmented reality, autonomous driving, and robotics. Traditional methods usually have challenges with complex indoor scenes made of multiple objects with different occlusions and orientations. In this work, the authors present an innovative model that integrates a new adaptive n-shifted shuffle (ANSS) attention mechanism with the Generalized Hough Transform (GHT) for robust 3D instance segmentation of indoor scenes. The proposed technique leverages the n-shifted sigmoid activation function, which improves the adaptive shuffle attention mechanism, permitting the network to dynamically focus on relevant features across various regions. A learnable shuffling pattern is produced through the proposed ANSS attention mechanism to spatially rearrange the relevant features, thus augmenting the model's ability to capture the object boundaries and their fine-grained details. The integration of GHT furnishes a vigorous framework to localize and detect objects in the 3D space, even when heavy noise and partial occlusions are present. The authors evaluate the proposed method on the challenging Stanford 3D Indoor Spaces Dataset (S3DIS), where it establishes its superiority over existing methods. The proposed approach achieves state-of-the-art performance in both mean Intersection over Union (IoU) and overall accuracy, showcasing its potential for practical deployment in real-world scenarios. These results illustrate that the integration of the ANSS and the GHT yields a robust solution for 3D instance segmentation tasks.

This paper presents the annotated genomes of Salmonella Haifa, Salmonella Bangkok, and Salmonella Reading, which are uncommonly isolated from meat in South Africa. Despite their rarity in South Africa, these serotypes have been linked to several high-profile outbreaks in other parts of the world., Competing Interests: The authors declare no conflict of interest.

This study examined the impact of fermentation using Lactiplantibacillus plantarum ( L75 ) and Bifidobacterium longum ( BF ) on the total soluble solids (TSS), pH, TA, LAB survival, color properties, ascorbic acid content, total phenolic content (TPC), carotenoid components, and antioxidant properties of smoothies made from melon varieties (Cantaloupe, Honeydew, and Watermelon) separately with pumpkin leaves ( Cucurbita moschata and Cucurbita pepo ). For all smoothies, pH (r = -0.74) and TSS (r = -0.79) were inversely and strongly correlated with LAB counts, while LAB counts were positively correlated with TA (r = 0.87). Fermentation time (24 to 72 h) significantly ( p < 0.05) decreased the TSS (%), pH, and color properties of all smoothies fermented with L75 or BF , while TA increased. Fermenting Cantaloupe melon and C pepo leaves with L75 (CMCL75) for 24 h increased the ascorbic acid content to 3.8 mg/100 mL. The sensory panel scores were highest for Watermelon and C. moschata or C. pepo fermented with L75 or BF for 24 h. TPC concentration was highest in CMCL75 (70.76 mg of gallic acid per 100 mL) after 24 h. C. pepo leaves and Cantaloupe fermented with L75 (CPCL75) showed the highest concentration of total carotenoids (70.38 mg/100 mL), lutein (2.53 µg/100 mL), cis β-carotene (25.43 µg/100 mL), and trans β-carotene (620.37 µg/100 mL). In contrast, CMCL75 showed the highest concentration of zeaxanthin (0.70 mg/100 mL). This study demonstrated the potential of fermenting Cantaloupe and pumpkin leaves together with the L75 strain to produce non-dairy functional products.

Selection pressures found in the prevailing production environments have shaped the genetic structure of indigenous chickens we see today. Indigenous chickens, raised in villages, provide essential genetic resources and income for poverty alleviation by providing affordable protein. However, they are threatened by predators, emerging diseases, and market demand for ideal breeds and fast production which causes loss of their valuable traits. The lack of knowledge about genetic diversity and genetic mechanisms underlying adaptive variants may compromise the goal of conserving indigenous chicken breeds. The main insights of the study are that indigenous chickens are highly diversified, and environmental factors play a key role in enabling chicken adaptation and distribution. Genomic and spatial technologies have made it possible to explore the genetic structure and fully comprehend the mechanism underlying the local adaptation of indigenous chickens. These technologies can aid in creating programs that enhance productivity and promote climate-resilient breeds. This review explores the impact of natural selection on indigenous chicken, genetic diversity, population size, and the advancement of technologies in understanding local adaptation drivers. In conclusion, this review highlights the importance of studying the habitats and how this will guide in conserving local breeds in their intended production environment., Competing Interests: Declaration of competing interest All authors declare that they have no conflicts of interest., (Copyright © 2024. Published by Elsevier Inc.)

The purpose of this study was to investigate female South African school athletes' trust in their coaches in relation to their perceptions of the coaches' justice, benevolence, integrity, competence, commitment to coach, willingness to cooperate, and performance. A quantitative cross-sectional research design was used in this study. The results showed that there was a large correlation between trust in the coach and the following factors: perceived justice ( r  = .504, p  < .01), perceived integrity ( r  = .511, p  < .01), and perceived competence ( r  = .534, p  < .01). Furthermore, multiple regression analysis results revealed that perceived justice was the only significant predictor of trust in the coach as it had a higher beta value ( β  = .17, p  < .05) than the other variables. This study shows that coaches should demonstrate fairness in their decision-making by providing players with incentives, opportunities to play, friendly relationships, and places of preference., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The handling editor KB declared a past collaboration with the author AK., (© 2024 Mashilo and Kubayi.)

Microbes such as bacteria and fungi play important roles in nutrient cycling in soils, often leading to the bioavailability of metabolically important mineral elements such as nitrogen (N), phosphorus (P), iron (Fe), and zinc (Zn). Examples of microbes with beneficial traits for plant growth promotion include mycorrhizal fungi, associative diazotrophs, and the N 2 -fixing rhizobia belonging to the α, β and γ class of Proteobacteria. Mycorrhizal fungi generally contribute to increasing the surface area of soil-root interface for optimum nutrient uptake by plants. However, when transformed into bacteroids inside root nodules, rhizobia also convert N 2 gas in air into ammonia for use by the bacteria and their host plant. Thus, nodulated legumes can meet a high proportion of their N requirements from N 2 fixation. The percentage of legume N derived from atmospheric N 2 fixation varies with crop species and genotype, with reported values ranging from 50-97%, 24-67%, 66-86% 27-92%, 50-92%, and 40-75% for soybean ( Gycine max ), groundnut ( Arachis hypogea ), mung bean ( Vigna radiata ), pigeon pea ( Cajanus cajan ), cowpea ( Vigna unguiculata ), and Kersting's groundnut ( Macrotyloma geocarpum ), respectively. This suggests that N 2 -fixing legumes require little or no N fertilizer for growth and grain yield when grown under field conditions. Even cereals and other species obtain a substantial proportion of their N nutrition from associative and endophytic N 2 -fixing bacteria. For example, about 12-33% of maize N requirement can be obtained from their association with Pseudomonas , Hebaspirillum , Azospirillum, and Brevundioronas , while cucumber can obtain 12.9-20.9% from its interaction with Paenebacillus beijingensis BJ-18. Exploiting the plant growth-promoting traits of soil microbes for increased crop productivity without any negative impact on the environment is the basis of green agriculture which is done through the use of biofertilizers. Either alone or in combination with other synergistic rhizobacteria, rhizobia and arbuscular mycorrhizal (AM) fungi have been widely used in agriculture, often increasing crop yields but with occasional failures due to the use of poor-quality inoculants, and wrong application techniques. This review explores the literature regarding the plant growth-promoting traits of soil microbes, and also highlights the bottle-necks in tapping this potential for sustainable agriculture.

Background: Mannose-binding lectin (MBL) is a key component of the innate immune system that plays a crucial role in binding to the microbial sugar surface to recognize and eliminate pathogens by activating the complement system., Objective: To detect and quantify the MBL protein concentration and chicken MBL expression in selected chicken breeds in South Africa., Methods: Forty-five blood samples from three indigenous chicken breeds, Ovambo (OV = 9), Venda (VD = 9) and Potchefstroom Koekoek (PK = 9), and two exotic chicken breeds, Rhode Island Red (RIR = 9) and Lohmann Brown (LB = 9), were used for MBL protein concentration using enzyme-linked immunosorbent assay (ELISA) techniques. Also 20 liver samples from symptomatic two indigenous chicken breeds, OV (5) and PK (5), and two exotic chicken breeds, RIR (5) and LB (5), were used for MBL expression using quantitative polymerase chain reaction (qPCR) techniques. A general linear model was done using Tukey's multiple comparison post hoc test., Results: The findings revealed MBL protein concentration from 5.26 to 18.56 µg/mL. The LB breed had the lowest mean 6.40 ± 0.80 µg/mL, whereas the PK breed had the highest mean MBL concentration of 17.70 ± 0.24 µg/mL of MBL protein concentration. At 12, 25 and 35 weeks, the MBL proteins of OV, VD, PK, RIR and LB varied significantly at p ≤ 0.05. The mRNA MBL expression of OV and LB breeds showed a 1-fold decrease in MBL expression, while RIR showed a 2-fold increase in MBL expression, and the PK showed more than a 3-fold increase in MBL expression relative to the control. The least-squares means for OV, LB, PK and RIR mRNA MBL expression were 0.54 ± 0.19, 0.68 ± 0.30, 4.46 ± 2.76 and 2.89 ± 0.19 µg/mL, respectively., Conclusion: MBL protein was detected and quantified with distinct differences in concentration and expression levels  at the presence of mycoplasma gallisepticum among the sampled South African chicken breeds. This highlights the genetic diversity of MBL as a tool for disease prevention in South African chicken breeds., (© 2024 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd.)

Safe drinking water requires sound monitoring and maintenance of residual chlorine within drinking water distribution networks (DWDNs) to suppress possible microbial regrowth. However, in the developing world, DWDNs face unique challenges, including aging infrastructure, water pipes laid near or even aboveground thus exposing water to high temperature fluctuations, and relatively high organic loads. Therefore, safely maintaining sustainable residual chlorine levels and restricting the problems of hazardous disinfection by-products (DBPs) formation and of antimicrobial resistance (AMR), both tracing back to extensive chlorination, is a difficult exercise in those settings. Here, the temperature dependent bulk chlorine decay, i.e., the rate at which chlorine residual is consumed, was estimated for a typical DWDN system in South Africa. To this end, experimental assays were performed and a mathematical model was developed to predict chlorine levels within the DWDN under study. A direct relationship (R 2 = 0.99) between bulk chlorine decay and initial chlorine dosage was identified, with bulk chlorine decay following the first-order decay model. The bulk chlorine decay rate coefficient (Kb) and the reaction constant with the pipe walls (Kw) were experimentally estimated, with the first being the main chlorine consumer and the latter only slightly contributing to chlorine decay. EPANET was used to simulate the chlorine concentrations within the examined DWDN, while residual chlorine concentrations were modelled using COMSOL. The software programs were calibrated and validated using experimental results. The optimum liquid chlorine dosage was 5 mg L -1 , and this could maintain residual levels at 0.5 mg L -1 for 3500 min in the water distribution tanks. Yet, the residual chlorine levels at the distal end of the DWDN were below the recommended safety limits, suggesting the need for chlorine booster stations to supplement residual chlorine rather than further increasing chlorine initial dosages which will lead to unsafe chlorine levels at the proximal points and inevitably will increase DBPs in drinking water. This relatively high chlorine dosage reflects the overall poor quality of the raw water that feeds the drinking water treatment plant under study, which is consistent with the poor water quality of surface water in South Africa. Overall, this methodology can be replicated in DWDNs in South Africa and across the developing world, where similar challenges persist and ensure safe drinking water but not at the expense of DBPs formation and possibly AMR spreading., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Background: Empirical studies have indicated that cardiometabolic multimorbidity risk profiles are upsurging among the South African population. However, there is a limited number of studies that have been conducted to validate these findings in rural communities., Aim: To study the prevalence of cardiometabolic risk factors of females residing in rural Glendale in the ILembe District, South Africa., Methods: One hundred females (aged 18-40 years) voluntarily participated in a cross-sectional observational study. All participants completed an ISAK somatotype profiling, and measured their fasting cholesterol, glucose, blood pressure and resting heart rate., Results: Participants' average age, body mass index, stature, and body mass, were 28.34 ± 7.07 years, 29.5 ± 8.06 kg/m 2 , 157.26 ± 6.09 cm, and 72.9 ± 21.12 kg, respectively. The cohort had a mean waist circumference of 94.2 ± 22.1 cm, hip circumference of 108.4 ± 20.9 cm, and waist-to-hip ratio of 0.86. Participants' mean heart rate, systolic blood pressure, and diastolic blood pressure were 86.31 ± 8.68 bpm, 116.79 ± 16.34 mmHg, and 82.14 ± 10.87 mmHg, respectively. Eleven participants recorded a resting heart rate greater than 100 bpm. Average blood glucose, total cholesterol, low-density lipoprotein, and high-density lipoprotein recorded were 4.87 ± 1.26 mmol/L, 3.78 ± 0.94 mmol/L, 1.76 ± 1.86 mmol/L, and 1.31 ± 0.4 mmol/L. Eighty-three participants HDL-C were below the recommended normative value of 1.55 mmol/L., Conclusion: The average participant presented as overweight, with elevated diastolic blood pressure, and a resting heart rate that has been proven to increase one's cardiometabolic multimorbidity risk profile. Additionally, a small portion of the cohort were identified to be prediabetic and diabetic. Large proportion of participants had low HDL-C levels suggestive of poor cardiovascular disease protection.

Manganese oxide (α-MnO 2 ) nanoparticles are highly recognised for their use in supercapacitor applications. This study demonstrates the successful synthesis of flower-like and nanorods hollandite α-MnO 2 by a simple one-pot hydrothermal technique at various reaction times. The synthesised nanoparticles were characterised by various physicochemical and electrochemical characterisation techniques. The influence of the various reaction times on the structural and morphological properties was evaluated by X-ray diffraction (XRD) and scanning electron microscope. XRD patterns revealed that the synthesized MnO 2 nanoparticles are tetragonal structures with crystallite sizes ranging from 13.69 to 20.37 nm estimated from the Williamson-Hall method. Moreover, the functional groups and surface area were examined by Fourier transform infrared spectroscopy and Bruner-Emmert-Teller, respectively. Furthermore, the compositional elements were studied by X-ray photoemission spectroscopy and energy-dispersive X-ray spectroscopy. Finally, the electrochemical performances were studied using cyclic voltammetry, galvanostatic charge-discharge and electrochemical impedance spectroscopy (EIS). The GCD characteristics revealed that the optimised α-MnO 2 has a good capacitive behaviour, which predicts the potential application in energy storage. Electrochemical studies revealed that the 3 h-MnO 2 sample exhibited a superior electrochemical behaviour and demonstrated a high specific capacitance of 132 F/g at a current density of 1A/g., (© 2024. The Author(s).)

Annona senegalensis pers , Sutherlandia frutescens (L.), and Withania somnifera (L.) are abundant plants and widely distributed in the Limpopo, Gauteng, Kwazulu-Natal, North West and Mpumalanga provinces in South Africa. The three plants are among those used by traditional healers and herbalists in South Africa for the treatment of a variety of diseases, including cancer. The current study aimed at the phytochemical screening and characterization of volatile compounds from the three medicinal plants using GC-MS. The methanol leaf extracts were subjected to phytochemical screening using standard chemical tests to detect the presence of different classes of compounds. Volatile compounds were detected by GC-MS analysis, and detected compounds were identified by comparing the MS spectral data with those of compounds deposited in the NIST Library (NIST08). Phytochemical screening indicated the presence of different secondary metabolites such as alkaloids, quinones, steroids, cardiac glycosides, coumarins, and terpenoids in all plants. GC-MS chromatograms allowed the detection and identification of 19 volatile compounds among the three plants with known bioactivities that are important in the management of life-threatening diseases such as cancer and diabetes. The results confirm the leaves of Annona senegalensis , Sutherlandia frutescens , and Withania somnifera as sources of important phytochemicals and therefore justify their use for the treatment of various diseases by traditional healers.

The present study investigated the indigenous metal-tolerant bacterial populations in the mine-water microbiome. Our intention was to assess the effects of the metal concentrations in mine water on the bacterial community of mine waters. The bacterial communities in Vanadium and Gold mine-water samples were exposed to different heavy-metal Arsenic, Cadmium, Chromium, Nickel, Mercury and Vanadium at two different concentrations (5 and 25 mM). The 16S rRNA amplicon from mine waters were sequenced using the Illumina's NGS MiSeq platform. Data analysis revealed a high diversity in the bacterial populations associated with the different heavy metals at different concentrations. The taxonomic profiles obtained after the exposure were different in different salts, but mostly dominated by Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria and Firmicutes at variable relative abundance. Principal Component Analysis (PCoA) predicts the clear community shift after exposure with heavy metals salts and emergence of tolerant community depending upon the specific community present in the original mine water., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

This paper highlights the critical role of pH or proton activity measurements in environmental studies and emphasises the importance of applying proper statistical approaches when handling pH data. This allows for more informed decisions to effectively manage environmental data such as from mining influenced water. Both the pH and {H + } of the same system display different distributions, with pH mostly displaying a normal or bimodal distribution and {H + } showing a lognormal distribution. It is therefore a challenge of whether to use pH or {H + } to compute the mean or measures of central tendency for further environmental statistical analyses. In this study, different statistical techniques were applied to understand the distribution of pH and {H + } from four different mine sites, Metsämonttu in Finland, Felsendome Rabenstein in Germany, Eastrand and Westrand mine water treatment plants in South Africa. Based on the statistical results, the geometric mean can be used to calculate the average of pH if the distribution is unimodal. For a multimodal pH data distribution, peak identifying methods can be applied to extract the mean for each data population and use them for further statistical analyses., Competing Interests: Declaration of competing interest The authors declare that they have no competing interests., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Afrotropical regions have high bird diversity, yet few studies have attempted to unravel the prevalence of avian haemosporidia in conservation areas. The diversity and host specificity of parasites in biodiversity hotspots is crucial to understanding parasite distribution and potential disease emergence. We test the hypothesis that biodiverse regions are associated with highly diverse parasites. By targeting the cytochrome b ( Cytb ) gene, we molecularly screened 1035 blood samples from 55 bird species for avian haemosporidia infections to determine its prevalence and diversity on sites inside and adjacent to the Kruger National Park. Overall infection prevalence was 28.41%. Haemoproteus , Leucocytozoon, and Plasmodium presented prevalences of 17.39%, 9.24%, and 4.64%, respectively. One hundred distinct parasite lineages were detected, of which 56 were new lineages. Haemoproteus also presented the highest diversity compared to Leucocytozoon and Plasmodium with varying levels of specificity. Haemoproteus lineages were found to be specialists while Plasmodium and Leucocytozoon lineages were generalists. We also found a positive relationship between avian host diversity and parasite diversity, supporting an amplification effect. These findings provide insight data for host-parasite and co-evolutionary relationship models.

Objective: Currently, there is a gap in knowledge on what the key goalkeeper attributes are during talent identification and selection in soccer. Hence, the objective was to investigate South African coaches' perceptions regarding key attributes of talented youth soccer goalkeepers., Design: Cross-sectional survey., Methods: A total of 173 soccer coaches (130 men and 43 women; M age = 36.6 ± 10.4 years; M coaching experience = 8.4 ± 7.3 years) were purposively recruited to participate in the study. All of the participants completed the Talent Identification Questionnaire in Soccer - Goalkeepers (TIDQS-GK)., Results: The six-component solution identified by the principal component analysis accounted for 68.96% of the total variance. Coaches deemed psychological (e.g. concentration, bravery, self-discipline), physical (e.g. speed, flexibility), social (e.g. coachability, communication), technical (e.g. catching the ball, first touch), tactical (e.g. defensive organisation against set plays) and perceptual-cognitive skills (e.g. decision-making) as important factors in identifying talented goalkeepers., Conclusions: The study offers new insights into the key attributes that soccer coaches look for in talented goalkeepers such as concentration, bravery, speed, coachability and decision-making and could direct coaches towards more objective evaluation strategies for informing their decisions., (© 2024. The Author(s).)

Hazardous chemicals are transported on rail and road networks. In the case of accidental spillage or terror attack, civilian and military first responders must approach the scene equipped with appropriate personal protective equipment. The plausible manufacturing of chemical protective polymer material, from photocatalyst anatase titanium dioxide (TiO 2 ) doped low-density polyethylene (LDPE), for cost-effective durable lightweight protective garments against toxic chemicals such as 2-chloroethyl ethyl sulphide (CEES) was investigated. The photocatalytic effects on the physico-chemical properties, before and after ultraviolet (UV) light exposure were evaluated. TiO 2 (0, 5, 10, 15% wt) doped LDPE films were extruded and characterized by SEM-EDX, TEM, tensile tester, DSC-TGA and permeation studies before and after exposure to UV light, respectively. Results revealed that tensile strength and thermal analysis showed an increasing shift, whilst CEES permeation times responded oppositely with a significant decrease from 127 min to 84 min due to the degradation of the polymer matrix for neat LDPE, before and after UV exposure. The TiO 2 -doped films showed an increasing shift in results obtained for physical properties as the doping concentration increased, before and after UV exposure. Relating to chemical properties, the trend was the inverse of the physical properties. The 15% TiO 2 -doped film showed improved permeation times only when the photocatalytic TiO 2 was activated. However, 5% TiO 2 -doped film exceptionally maintained better permeation times before and after UV exposure demonstrating better resistance against CEES permeation.

Chromium electroplating produces Cr(III)-containing electroplating sludge (EPS) in large volumes, which is easily oxidised to Cr(Ⅵ) and is harmful to the environment and human health. This study recovered Cr(III) as Na 2 CrO 4 from EPS using an integrated bipolar membrane electrodialysis (BMED)-H 2 O 2 oxidation technology. During the treatment process, Cr(III) was oxidised to Cr(VI) using H 2 O 2 in an alkaline environment, BMED was used to separate and recover Cr(VI). Experimental results showed that H 2 O 2 dosage and pH affected Cr(III) oxidation-the highest Cr(III) oxidation ratio of 68.4% was observed when H 2 O 2 dosage and pH were 5.5 mL and 12.0, respectively. The current density, solid/liquid ratio and sludge particle size affected Cr(III) recovery, energy consumption and current efficiency. Under a current density of 20.0 mA/cm 2 , solid/liquid ratio of 1.0:45 and sludge particle size of 100 mesh, 58.2% of Cr(III) was recovered. When the number of the equipped EPS compartments was increased from one to two and three, the specific energy consumption decreased from 1.04 to 0.87 and 0.81 kW h/g, respectively, but the current efficiency remained almost constant. After EPS treatment, the Cr(III) remaining in the sludge was mainly in the residual state, which is less environmentally harmful. The obtained Na 2 CrO 4 had similar properties according to X-ray diffraction analysis. Thus, the proposed integrated technology effectively recovers Cr(III) from EPS and other chromium-containing solid wastes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Monolayer boron nanosheet, commonly known as borophene, has garnered significant attention in recent years due to its unique structural, electronic, mechanical, and thermal properties. This review paper provides a comprehensive overview of the advancements in the synthetic strategies, tunable properties, and prospective applications of borophene, specifically focusing on its potential in energy storage devices. The review begins by discussing the various synthesis techniques for borophene, including molecular beam epitaxy (MBE), chemical vapor deposition (CVD), and chemical methods, such as ultrasonic exfoliation and thermal decomposition of boron-containing precursors. The tunable properties of borophene, including its electronic, mechanical, and thermal characteristics, are extensively reviewed, with discussions on its bandgap engineering, plasmonic behavior, and thermal conductivity. Moreover, the potential applications of borophene in energy storage devices, particularly as anode materials in metal-ion batteries and supercapacitors, along with its prospects in other energy storage systems, such as sodium-oxygen batteries, are succinctly, discussed. Hence, this review provides valuable insights into the synthesis, properties, and applications of borophene, offering much-desired guidance for further research and development in this promising area of nanomaterials science., (© 2024 The Author(s). Small published by Wiley‐VCH GmbH.)

Ethnopharmacological Relevance: South Africa harbours a large number of Bulbine (Xanthorrhoeaceae) species, which includes ethnobotanically important indigenous species. Traditionally, Bulbine leaves are used by several ethnic groups in South Africa to treat dermatological conditions including wounds, which led to the development of Bulbine-containing cosmetic products. However, scientific evidence is needed to support the claims in treating skin conditions and wound-healing., Aim of the Study: This comparative study was undertaken to investigate the wound-healing properties of five Bulbine species indigenous to South Africa, using in vitro and in vivo models., Materials and Methods: Five Bulbine species, B. abyssinica, B. asphodeloides, B. frutescens, B. latifolia and B. narcissifolia were collected from natural populations in the Eastern Cape Province of South Africa. The chemical profiles of the methanol leaf extracts were acquired using ultra-performance liquid chromatography with photodiode array detection in tandem with quadrupole time-of-flight mass spectrometry. The methyl thiazolyl tetrazolium (MTT) assay and maximum tolerated concentration (MTC) assay were used to assess the in vitro and in vivo toxicity of the extracts, respectively. The in vitro scratch assay was employed to monitor cell migration and wound-closure in a HaCaT cell monolayer, following treatment with the plant extracts for 48 h. In vivo wound-healing potential was determined using the zebrafish larvae caudal fin amputation assay, assessed in three-days post fertilization larvae and various concentrations of the plant extracts were tested in both assays to determine the concentration-response effect. Data were analysed using MS Excel® enhanced with the Real Statistics add-in., Results and Discussion: Using UPLC-MS, 11 major compounds were tentatively identified in the five Bulbine species. Although the compounds varied between species, all five Bulbine species contained the phenylanthraquinone, knipholone. Kaempferol glucoside was identified in four species, but not in B. abyssinica. The five Bulbine species were non-cytotoxic (cell viability > 80%) towards keratinocytes at all three tested concentrations. However, B. latifolia was toxic towards zebrafish larvae at all the tested concentrations, while the other four species were non-toxic at low concentrations. The results of the scratch assay revealed that B. abyssinica was the most active extract at 100 μg/mL. Compared to the untreated control, wound-closure notably increased by 28% (p < 0.05), 44% (p < 0.01) and 34% (p < 0.05) after 12 h, 24 h and 36 h post-treatment, respectively. Although none of the species achieved 100% caudal fin regeneration by the end of the treatment period, B. frutescens demonstrated the highest regeneration (90%) and most significant difference (p < 0.01) compared to the untreated control., Conclusion: The results revealed that the five Bulbine species have complex chemical profiles, however, they share major compound classes (i.e. phenylanthroquinones and flavonoid analogues) across the species. The study highlights the wound-healing properties of the five species, which is consistent with their traditional use., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. A Viljoen declares his role as Editor-in-Chief of JEP and the submission will obviously be handled completely by one of the associate editors., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Ethnopharmacological Relevance: Lobostemon fruticosus (L.) H.Buek is a perennial and woody shrub of the Boraginaceae family, found in the Cape region of South Africa. The leaves and twigs are used to treat dermatological conditions such as wounds, burns, ringworm, erysipelas and eczema. Anti-inflammatory, antibacterial, antiviral and anti-proliferative activities of L. fruticosus have been reported. However, there is a void in research which reports on the wound healing properties of this plant., Aim of the Study: Aligned with the traditional use of L. fruticosus, our study aimed to use in vitro and in vivo bioassays to confirm the wound healing potential of the plant., Materials and Methods: An aqueous methanol extract (80% v/v) of L. fruticosus was prepared using a sample collected from the Western Cape Province of South Africa and chromatographically profiled by ultra-performance liquid chromatography coupled to mass spectrometry (UPLC-MS). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay was performed to determine the non-toxic concentrations of the extract for subsequent use in the in vitro scratch assay. Both the human keratinocyte (HaCaT) and fibroblast (BJ-5ta) cell lines were employed in the in vitro scratch assay. The in vivo caudal fin amputation assay was used to assess the wound healing potential of L. fruticosus, by monitoring fin regeneration in zebrafish larvae treated with the plant extract at various concentrations., Results: Six major compounds were tentatively identified in the L. fruticosus extract namely; globoidnan A, globoidnan B, rutin, rabdosiin, sagerinic acid and rosmarinic acid. The potentially toxic pyrrolizidine alkaloids were also identified and quantitatively confirmed to be present at a low concentration of 119.58 ppm (m/m). Treatment of HaCaT and BJ-5ta cells with the plant extract in the scratch assay resulted in an increase in cell migration, which translates to accelerated wound closure. After 24 hr treatment with 100 μg/mL of extract, wound closure was recorded to be 91.1 ± 5.7% and 94.1 ± 1.3% for the HaCaT and BJ-5ta cells, respectively, while the untreated (medium) controls showed 72.3 ± 3.3% and 73.0 ± 4.3% for the two cell lines, respectively. Complete wound closure was observed between 24 and 36 hr, while the untreated control group did not achieve 100% wound closure by the end of the observation period (48 hr). In vivo, the crude extract at 100 μg/mL accelerated zebrafish caudal fin regeneration achieving 100.5 ± 3.8% regeneration compared to 68.3 ± 6.6% in the untreated control at two days post amputation., Conclusions: The study affirms the wound healing properties, as well as low toxicity of L. fruticosus using both in vitro and in vivo assays, which supports the traditional medicinal use. Other in vitro assays that target different mechanisms involved in wound healing should be investigated to support the current findings., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. No conflicts to declare. A Viljoen declares his role as Editor-in-Chief of JEP and the submission will obviously be handled completely by one of the associate editors., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

The growing frequency and severity of disasters worldwide have highlighted the need for environmental health practitioners to be equipped with specialised training to respond effectively to evolving public health contexts. Disasters can have long-lasting impacts on the environment and environmental health services, necessitating prompt and effective responses. However, the current environmental health workforce faces challenges in acquiring the necessary competencies to address environmental health threats during disasters. This narrative review synthesises existing literature on disaster management education for environmental health professionals, exploring current training, advancements and emerging trends. The review follows Preferred Reporting Items for Systematic Reviews and Meta-analyses guidelines and includes a total of 45 records that met inclusion criteria (compromising 15 peer-reviewed articles and 30 training records) published between 2002 and 2023. Findings highlight the expansion of environmental health degree programmes to include disaster management, better preparing newly qualified practitioners. However, a knowledge gap remains for previously qualified practitioners. High-income countries prioritising capacity building for environmental health practitioners in disaster management are better equipped to respond to and mitigate disasters., Contribution: The review suggests that with proper basic training for disaster responders, more lives can be saved during and after disasters. It highlights the insufficiency of current training programmes and emphasises the need for advanced role-specific training for environmental health practitioners. The review emphasises the need for advanced role-specific training, community assessment skills and focused disaster response strategies to enhance environmental health practitioners' ability to respond to disasters and improve public health resilience. Enhanced training, capacity building and collaboration are necessary to improve the competencies, skills and knowledge of environmental health practitioners in disaster risk management and public health emergencies., Competing Interests: The authors declare that they have no financial or personal relationship(s) that may have inappropriately influenced them in writing this article., (© 2024. The Authors.)

Quinoline-urea-benzothiazole hybrids exhibited low to sub-micromolar in vitro activities against the Plasmodium falciparum ( P. falciparum ) 3D7 chloroquine (CQ)-sensitive strain, with compounds 5a , 5b and 5f showing activities ranging from 0.33 to 0.97 μM. Against the formation of β-haematin, the majority of the tested compounds were comparable to the reference drug, chloroquine (CQ), with compounds 5c (IC 50  = 9.55 ± 0.62 μM) and 5h (IC 50  = 9.73 ± 1.38 μM), exhibiting slightly better in vitro efficacy than CQ. The hybrids also exhibited low micromolar to submicromolar activities against Trypanosoma brucei brucei , with 5j - 5k being comparable to the reference drug, pentamidine. Compound 5b displayed higher in silico binding energy than CQ when docked against P. falciparum dihydroorotate dehydrogenase enzyme. Compounds 5j and 5k showed higher binding energies than pentamidine within the trypanothione reductase enzyme binding pocket. The root means square deviations of the hit compounds 5b, 5j and 5k were stable throughout the 100 ns simulation period. Post-molecular dynamics MMGBSA binding free energies showed that the selected hybrids bind spontaneously to the respective enzymes. The DFT investigation revealed that the compounds have regions that can bind to the electropositive and electronegative sites of the proteins., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

The Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) aims to prevent the overexploitation of species by controlling their trade. However, there is currently no international regulatory framework to protect the trade of non-CITES species. We examined the LEMIS database, online trade, and scientific literature with the aim of identifying and compiling a list of South African native species traded as pets and wildlife products. We found that there are 223 non-CITES species traded as wildlife products and 95 species traded as pets. Mammals and birds were the most traded taxa for wildlife products, while reptiles and amphibians were mostly traded as pets. At the least, species traded as wildlife products and pets are currently not facing extinction, as most are categorized as Least Concern. However, some endemic species have an unknown population size, with Sclerophrys pantherina and Neamblysomus gunningi being Endangered. The international pet trade involves 10 countries, with the USA, the Czech Republic, and the UK being the largest importers. The trade of species as wildlife products involves 20 countries, with the USA being the major importer. This study emphasizes the necessity of strict regulations and international cooperation to control the wildlife trade effectively.

Carbon black, a nano-porous material usually derived from the pyrolysis of waste tyres possesses varied particle sizes and morphology making it a viable material for several engineering applications. However, the high tendency for CB to agglomerate remains a challenge. To address this, bio-templating has been employed to produce a nanostructured porous carbon electrode material for supercapacitor applications using diatomite as a template. Diatomite-synthesized activated carbon (DSAC) was fabricated through a three-step process involving acid treatment of diatomite, thermal activation of carbon black, and bio-template synthesis. The resulting material was thoroughly characterized using XRD, Raman spectroscopy, BET analysis, and SEM imaging. Its electrochemical properties were assessed through cyclic voltammetry, galvanostatic charge-discharge, and electrochemical impedance spectroscopy. The DSAC material exhibited a high specific surface area of 266.867 m 2 g -1 , pore volume of 0.6606 cm 3 g -1 , and mean pore radius of 1.8943 nm. The electrochemical evaluation revealed that DSAC demonstrates excellent electrochemical performance, achieving a high specific capacitance of 630.18 F g -1 and retaining 94.29% capacitance after 5000 cycles at 1 A g -1 . The DSAC electrode is eco-friendly and a promising candidate for supercapacitor applications., Competing Interests: The authors declare no conflict of interest, financial or otherwise., (This journal is © The Royal Society of Chemistry.)

Background: This study comparatively investigates the anthropometric and physical fitness characteristics of male South African players by age group, competitive level and playing position., Methods: The sample consisted of 201 youth soccer players drawn from six sports academies in South Africa. Players were subjected to standardized protocols for anthropometric measurements (stature, body mass and body fat percentage) and physical fitness tests (flexibility, vertical jump, push-ups, sit-ups, muscle strength, agility, 30-m sprint and V̇O2max). A series of two-way analyses of variance was performed to determine the interactions between playing position (goalkeeper, defender, midfielder or attacker) or age group (13, under-15, under-17 or under-19) and competitive level (elite or non-elite)., Results: The results show significant interaction effects of agility, body fat percentage and sprint speed between the players' competitive levels and age group (P<0.05). No interaction effect was found between playing position and competitive level in any anthropometric or physical fitness variable (P>0.05). However, playing position exhibited significant main effects in the youth players' aerobic endurance and body mass (P<0.05)., Conclusions: The results provide practical implications for tailoring position-specific conditioning regimes that optimize effective soccer performance.

Global trade depends on long-haul transportation, yet comfort for drivers on lengthy trips is sometimes neglected. Rough roads have a major negative influence on driver comfort and increase the risk of weariness, distracted driving, and accidents. Using Random Forest regression, a machine learning technique well-suited to examining big datasets and nonlinear relationships, this study examines the relationship between road roughness and driver comfort. Using the MIRANDA mobile application, data were gathered from 1,048,576 rows, including vehicle acceleration and values for the International Roughness Index (IRI). The Support Vector Regression (SVR) and XGBoost models were used for comparative analysis. Random Forest was preferred because of its ability to be deployed in real time and use less memory, even if XGBoost performed better in terms of training time and prediction accuracy. The findings showed a significant relationship between driver discomfort and road roughness, with rougher roads resulting in increased vertical acceleration and lower comfort levels (Road Roughness: SD-0.73; Driver's Comfort: Mean-10.01, SD-0.64). This study highlights how crucial it is to provide smooth surfaces and road maintenance in order to increase road safety, lessen driver weariness, and promote long-haul driver welfare. These results offer information to transportation authorities and policymakers to help them make data-driven decisions that enhance the efficiency of transportation and road conditions.

The DC-DC converter is an essential subsystem in electric vehicle (EV) chargers, and most converters depend on a single-input single-output structure, which can be costly when multiple charging units are needed. Additionally, these converters offer limited voltage gain, restricting charging configurations. This paper proposed a dual-stage high-gain converter that operates in a boost mode with reduced components, using an inductor, capacitor, and 2-diodes (LC2D) to provide high-gain output. The proposed Dual Inputs and Dual Outputs (DIDO) converter tied with Photovoltaic (PV) and constant DC acting as inputs and two different voltage levels EV chargers are serving as the output. The proposed converter operates at continuous conduction mode (CCM), achieving high efficiency and reliability with fewer losses. The proposed work was designed for 418V and 85V systems in MATLAB/Simulink, and the results were validated with hardware implementation. The proposed converter delivers high-gain output to the electric vehicle application with 92.2 % efficiency., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 Published by Elsevier Ltd.)

Cardiovascular disease (CVD) risk factors are frequently reported among firefighters, yet no studies have compared these factors between male and female firefighters, specifically from a low- to middle-income country (LMIC). This study aimed to determine the prevalence of CVD risk factors and their relationship with cardiorespiratory fitness (VO 2max ) in 254 active career firefighters (mean age: 42.6 ± 7.8 years). The assessments included anthropometry, blood pressure, blood glucose, cholesterol, triglycerides, and VO 2max . The results indicated that 48.0% and 51.8% of females and males were pre-hypertensive, respectively. Hypertension was identified in 15.8% of the firefighters. According to body mass index (BMI), 37.3% of males and 25% of females were found to be overweight, while an additional 44.9% of males and 45.7% of females were classified as obese. Only 17.3% of males and 18.2% of females were found to be of normal weight. These findings were corroborated by categories of central obesity using waist circumference (WC), which were 47.7% for males and 41.6% for females. Low HDL-C was found in 95.2% of males and 86.4% of females, with 28.3% of males also having elevated triglyceride levels (TG). VO 2max was "excellent" in 48.8% of males and 12.6% of females, though it had no significant association with most CVD risk factors. The only notable link was a small correlation between VO 2max and triglycerides (r = -0.215; p = 0.001). These findings suggest that while cardiorespiratory fitness may have no impact, additional factors likely contribute to the cardiovascular health of firefighters, necessitating the need for comprehensive health and fitness programmes.

Background: Occupational nurses continue to provide service to mineworkers diagnosed with chronic illnesses, however, non-adherence to medication is prevalent, cause overcrowding, long queues and admission at hospitals as they become sick., Aim: This study aimed to describe the experience of occupational nurses (ONs) regarding non-adherence to medication among mineworkers diagnosed with chronic illnesses., Setting: The study was conducted at a selected mine in Gauteng province, South Africa., Methods: A qualitative, exploratory, descriptive design that is contextual in nature, was used with a phenomenological approach. Thirteen ONs were purposively sampled and individual face-to-face interviews were conducted until data saturation was reached. Thematic analysis using ATLAS.ti 24 software was applied to analyse the data collected. The researcher and the independent coder held a consensus discussion and agreed on the themes and the sub-themes., Results: Two themes and various sub-themes emerged along with Care-related challenges linked to the mineworkers and challenges related to the provision of service to mineworkers. Data analysed indicated that the ONs had varying but often similar perspectives regarding non-adherence to medication among mineworkers diagnosed with chronic illnesses., Conclusion: Non-adherence to medication is prevalent among mineworkers diagnosed with chronic illnesses and improving the mineworkers' outcomes requires addressing the issue of non-adherence to primary medication., Contribution: This study highlights the importance of adhering to prescribed medication among mineworkers diagnosed with chronic illnesses to ensure quality of life., Competing Interests: The authors declare that they have no financial or personal relationships that may have inappropriately influenced them in writing this article., (© 2024. The Authors.)

Future viability depends on ensuring a sustainable society because green energy methods may efficiently reduce greenhouse gas emissions. Nevertheless, stakeholders, consumers, and developers continue to be notably ignorant of the financial incentives connected to green technology. Moreover, there is still a dearth of studies on the range of financial incentives offered by different authorities in India. Monetary incentives, such as tax breaks, indirect tax exemptions, and refunds, are crucial in encouraging the use of green technology in the modern world. This study explores the importance of financial incentives for green building technologies in India, which also looks at the wide range of incentives provided by federal, state, and local governments. Furthermore, the study highlights various state government programs such as goods subsidies, exemptions from local taxes, and fee waivers. Notably, several incentives aimed at consumers, developers, and other stakeholders have been implemented by the Indian Green Building Council (IGBC). This review study emphasizes the effectiveness of financial incentives in green construction projects and draws attention to a clear knowledge gap regarding the adoption of green technology. This study also provides insights into potential future directions. Studies and research results emphasize the importance of spreading the word about financial incentives as a key factor in determining the adoption of green technologies. Many parties, including governmental organizations, municipal governments, developers, and clients engaged in green building technology projects, stand to gain increased awareness., Competing Interests: No competing interests were disclosed., (Copyright: © 2024 Kumar R et al.)

Decoding semantic concepts for imagination and perception tasks (SCIP) is important for rehabilitation medicine as well as cognitive neuroscience. Electroencephalogram (EEG) is commonly used in the relevant fields, because it is a low-cost noninvasive technique with high temporal resolution. However, as EEG signals contain a high noise level resulting in a low signal-to-noise ratio, it makes decoding EEG-based semantic concepts for imagination and perception tasks (SCIP-EEG) challenging. Currently, neural network algorithms such as CNN, RNN, and LSTM have almost reached their limits in EEG signal decoding due to their own short-comings. The emergence of transformer methods has improved the classification performance of neural networks for EEG signals. However, the transformer model has a large parameter set and high complexity, which is not conducive to the application of BCI. EEG signals have high spatial correlation. The relationship between signals from different electrodes is more complex. Capsule neural networks can effectively model the spatial relationship between electrodes through vector representation and a dynamic routing mechanism. Therefore, it achieves more accurate feature extraction and classification. This paper proposes a spatio-temporal capsule network with a self-correlation routing mechaninsm for the classification of semantic conceptual EEG signals. By improving the feature extraction and routing mechanism, the model is able to more effectively capture the highly variable spatio-temporal features from EEG signals and establish connections between capsules, thereby enhancing classification accuracy and model efficiency. The performance of the proposed model was validated using the publicly accessible semantic concept dataset for imagined and perceived tasks from Bath University. Our model achieved average accuracies of 94.9%, 93.3%, and 78.4% in the three sensory modalities (pictorial, orthographic, and audio), respectively. The overall average accuracy across the three sensory modalities is 88.9%. Compared to existing advanced algorithms, the proposed model achieved state-of-the-art performance, significantly improving classification accuracy. Additionally, the proposed model is more stable and efficient, making it a better decoding solution for SCIP-EEG decoding.

Ethnopharmacological Relevance: The use of medicinal plants for central nervous system (CNS)-related ailments, such as epilepsy and anxiety, is prevalent in South Africa. Plants from the Lamiaceae family are commonly used for their therapeutic benefits. Leonotis leonurus (L.) R.Br. has been reported in ethnobotanical literature to have anticonvulsant and anxiolytic effects through the inhalation of pyrolysis products obtained by combustion of the aerial parts., Aim and Objectives: To explore the chemical profiles and CNS activity of the smoke extract and isolated constituents of L. leonurus in zebrafish larvae, through anticonvulsive and anxiolytic activity assays., Materials and Methods: The smoke extract of L. leonurus was obtained through the combustion of the aerial parts of the plant using a custom-built smoke recovery apparatus. The chemical profile of the smoke constituents was determined using Ultra-Performance Liquid Chromatography coupled with Mass Spectrometry (UPLC-MS). Targeted compounds were subjected to preparative High-Performance Liquid Chromatography for separation before structure elucidation using Nuclear Magnetic Resonance (NMR). The maximum tolerated concentrations, as well as the anxiolytic activity of the smoke extract were determined in five days post fertilisation zebrafish larvae. Reverse-thigmotaxis and locomotor activity of larvae in the light/dark transition assay were used to determine anxiolytic activity. Zebrafish larvae at six days post fertilisation (dpf) were subjected to several concentrations of the smoke constituents of L. leonurus. The baseline locomotor activity of the larvae was tracked for 30 min, prior to addition of pentylenetetrazole (PTZ) to induce seizure-like behaviour in the larvae, after which the locomotor activity of the larvae was once again tracked for an additional 30 min., Results: The UPLC-MS profiles of the smoke extract revealed the presence of two main compounds, leoleorin A and leoleorin B, which were targeted and isolated. Upon subjection to NMR spectroscopy for structure elucidation, the compounds were confirmed to be labdane diterpenoids. Both leoleorin A and leoleorin B, and the smoke extract displayed suppression of the PTZ induced seizure-like behaviour in zebrafish larvae. Under light and dark conditions, the smoke extract and compounds displayed potential anxiolytic activity at different concentrations., Conclusion: Our results suggest that the smoke constituents of L. leonurus may exert anticonvulsant and anxiolytic effects which align with the traditional indications and the mode of administration., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. No conflicts to declare. A Viljoen has declared his role as Editor-in-Chief of JEP and the submission will obviously be handled completely by one of the associate editors., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Background: This study investigates the effects of manipulating the number of ball touches (free play vs. two touches) on the physical and technical actions of elite male youth soccer players during 5v5 + 2 goalkeepers (GKs) small-sided games (SSGs)., Methods: Players played in two different SSGs: 1) free play, where the number of touches per possession was not restricted; and 2) two touches, where players were limited to a maximum of two touches per ball possession. A total of 24 male elite youth soccer players (age: 14.79±0.71 years; body mass: 56.02±1.41 kg; body height: 164±2.12 cm) participated in the study. Players' physical metric and technical performance data were captured using a global positioning system and video camera, respectively. A paired-samples t-test or Wilcoxon Signed-Rank Test was employed to examine differences in players' technical performance variables depending on SSGs' ball-touch rules., Results: The results show significantly more unsuccessful passes (t=-3.48; P=0.04; d=1.92) when players were limited to two touches than when there were no pass limits. The physical metrics indicate that total distance covered (Z=-2.90; P=0.001; d=0.07), meters per minute (Z=-3.44; P=0.001; d=0.11), low-speed running (Z=-2.25; P=0.02; d=0.04) and high acceleration (Z=-1.90; P=0.05; d=0.28) were significantly higher when touches were unlimited than when they were not., Conclusions: Soccer coaches should decide the number of touches per ball possession they allow depending on their tactical and/or physical objectives in training.

The disposal of coal fly ash (CFA) generated from coal-fired power stations has serious impact on the ecosystem, by converting large pieces of land to barren ash dams with the potential to contaminate groundwater, surface water, air and soil. The aim of this study was to clarify the potential of phytoremediation using Helichrysum splendidum (Thunb.) Less. in areas polluted by CFA through conduction of pot trial experiments for 14 weeks. Plants of the same age were cultivated in CFA to assess their growth, photosynthetic rate and tolerance towards metal toxicity. This study revealed that the CFA was moderately polluted with heavy metals, and a lower photosynthetic rate was recorded for the CFA plants in comparison to the controls (plants grown in soil). Although the CO 2 assimilation rate was lower for the CFA plants, increased growth was recorded for all the plants tested. Inductively coupled plasma mass spectrometry (ICP-MS) was used to quantify the amount of trace elements in samples and parameters including translocation factor (TF) and bioconcentration factor (BCF) were used to evaluate the phytoremediation potential of H. splendidum (Thunb.) Less. The results revealed that higher concentrations of Cd, Co, Cr, Cu, Mn and Pb were accumulated in the roots, while As, Ni and Zn were found in the shoots. Elements including As, Cr and Zn reported TF values above 1, indicating the plants' phytoextraction potential. The BCF values for As, Cu and Zn were 1.22, 1.19 and 1.03, indicating effectiveness in the phytostabilization processes. A removal rate efficiency ranging from 18.0 to 56.7% was recorded confirming that, H. splendidum (Thunb.) Less. can be employed for restoration of CFA dams.

Unripe banana flour starch possesses a high degree of resistance to enzymatic hydrolysis, a unique and desirable property that could be exploited in the development of functional food products to regulate blood sugar levels and promote digestive health. However, due to a multifactorial phenomenon in the banana flour matrix-from the molecular to the micro level-there is no consensus regarding the complex mechanisms behind the slow enzymatic hydrolysis of unripe banana flour starch. This work therefore explores factors that influence the enzymatic hydrolysis resistance of raw and modified banana flour and its starch including the proportion and distribution of the amorphous and crystalline phases of the starch granules; granule morphology; amylose-amylopectin ratio; as well as the presence of nonstarch components such as proteins, lipids, and phenolic compounds. Our findings revealed that the relative contributions of these factors to banana starch hydrolytic resistance are apparently dependent on the native or processed state of the starch as well as the cultivar type. The interrelatability of these factors in ensuring amylolytic resistance of unripe banana flour starch was further highlighted as another reason for the multifactorial phenomenon. Knowledge of these factors and their contributions to enzymatic hydrolysis resistance individually and interconnectedly will provide insights into enhanced ways of extraction, processing, and utilization of unripe banana flour and its starch., (© 2024 Institute of Food Technologists.)

The debate on tuition-free higher education has raged in South Africa since the #FeesMustFall protests at South African universities in 2015 and 2016. The government responded with a tuition-free education policy targeting students from households earning less than R350 000. However, the question remains can South Africa sustain a tuition-free education policy given its developing nation status and the levels of its GDP? This article sought to assess the feasibility of tuition-free higher education for all in South Africa. A scoping review was used, and fifteen articles about tuition-free higher education feasibility in South Africa were reviewed. The choice of the scoping review was due to the need for an understanding of the current state of play in research on the feasibility of tuition-free higher education in South Africa. The results suggest that tuition-free higher education for all is not feasible in South Africa. There seems to be a consensus that South Africa lacks the resources to finance tuition-free higher education for all. Tuition-free higher education is also viewed as a regressive tax on the poor given that the majority of students in higher education institutions come from middle and upper-income households. However, it is important to note that a distinction is drawn between tuition-free higher education for all and tuition-free higher education for the poor and academically deserving. The latter seems to receive support with some viewing it as a moral imperative in an unequal society such as South Africa's. It is recommended that future studies approach the issue from an empirical standpoint whereby the GDP levels are assessed against higher education funding., Competing Interests: No competing interests were disclosed., (Copyright: © 2024 Ramasu T and Kanakana-Katumba G.)

Access to safe drinking water sources and appropriate sanitation facilities remains a dream in low and middle-income countries including South Africa. This study identified the origin of faecal pollution by using quantitative polymerase chain reaction (qPCR) targeting host-specific Bacteroidales genetic markers to track the distribution of human-specific (BacHum) and animal-specific (cattle-BacCow, chicken-Cytb, pig-Pig-2-Bac, dog-BacCan) markers in water sources used by rural communities of the Vhembe District Municipality (VDM). Results revealed the prevalence of BacHum, BacCow, and BacCan in all surface water sources in Thulamela Local Municipality (TLM) and Collins Chabane Local Municipality (CLM) during wet (100%) and dry seasons (50-75%). Cytb was not detected in untreated spring water in TLM and CLM, and Pig-2-Bac was not detected in untreated hand-dug well water in TLM during both seasons. Household-level analysis detected Cytb (28.8% wet, 17.5% dry), BacHum (34.4% wet, 25% dry for Pig-2-Bac) in stored untreated spring water in CLM, and Cytb (42.9% wet, 28.5% dry) in untreated hand-dug well water in TLM. Despite differences in detection frequencies of host-specific Bacteroidales, the study highlights the public health concern of faecal pollution in rural VDM households., (© 2024. The Author(s).)

Baby spinach is becoming increasingly popular as a salad ingredient and needs high fertiliser rates to grow well and attain higher-quality leaves (dark green leaves). Chemical fertilisers, especially nitrogen (N), boost yields. There are many risks associated with nitrogen fertilisation. Additionally, spinach contains phenolic compounds and carotenoids. Nitrogen fertilisation affects growth, development, yield and metabolites. This study examined the impact of lower concentrations of N (0, 30, 60, 90, 120, 150 mg/L) on yield and colour properties [light intensity (L*) colour coordinates, unique for green colour (a*) and yellow colour (b*)], as well as the impact of varying N concentrations on the total phenolic content and p-coumaric acid, quercetin, ferulic acid, kaempferol, lutein, zeaxanthin, β-carotene and antioxidant activities in the baby spinach varieties 'Acadia', 'Crosstrek' and 'Traverse', and it was established that N fertilisation improves phytochemical bioaccessibility and antioxidant activity. In a split strip plot design, three baby spinach varieties were treated with different N concentrations, including 0, 30, 60, 90, 120 and 150 mg/L. For 40 days, three baby spinach varieties were grown on soilless Mikskaar Professional substrate 300. During both seasons, 'Crosstrek' had the highest fresh mass (921.4 g/m 2 , 856.3 g/m 2 ) at 120 mg/L N, while 'Traverse' had the highest fresh mass at 554.8 g/m 2 and at 564.3 g/m 2 at 90 mg/L N and did not differ significantly from 90 to 150 mg/L N during either season. During both seasons, 'Acadia' at 90 mg/L N increased fresh mass to 599 g/m 2 and 557.9 g/m 2 . The variety × N supply interaction significantly affected the leaf colour; chlorophyll content across seasons; the levels of bioactive compounds, p-coumaric acid, quercetin, ferulic acid, kaempferol, lutein, zeaxanthin and β-carotene in spinach varieties; the in vitro bioaccessibility; and the antioxidant activity. Varietal differences influenced the bioaccessibility of phenolic compounds and carotenoid components. The appropriate N levels can be used during plant cultivation to optimise the bioaccessibility of this spinach variety. Thus, fertilising 'Traverse' with 90 mg/N mL increased the in vitro bioaccessibility of β-carotene (35.2%), p-coumaric acid (7.13%), quercetin (8.29%) and ferulic acid (1.92%) without compromising the yield.

Background: Prostate Cancer (PCa) is the second most diagnosed urological cancer among men worldwide. Conventional methods used for diagnosis of PCa have several pitfalls which include lack of sensitivity and specificity. On the other hand, traditional treatment of PCa poses challenges such as long-term side effects and the development of multidrug resistance (MDR)., Main Body: Hence, there is a need for novel PCa agents with the potential to lessen the burden of these adverse effects on patients. Nanotechnology has emerged as a promising approach to support both early diagnosis and effective treatment of tumours by ensuring precise delivery of the drug to the targeted site of the disease. Most cancer-related biological processes occur on the nanoscale hence application of nanotechnology has been greatly appreciated and implemented in the management and therapeutics of cancer. Nuclear medicine plays a significant role in the non-invasive diagnosis and treatment of PCa using appropriate radiopharmaceuticals. This review aims to explore the different radiolabelled nanomaterials to enhance the specific delivery of imaging and therapeutic agents to cancer cells. Thereafter, the review appraises the advantages and disadvantages of these modalities and then discusses and outlines the benefits of radiolabelled nanomaterials in targeting cancerous prostatic tumours. Moreover, the nanoradiotheranostic approaches currently developed for PCa are discussed and finally the prospects of combining radiopharmaceuticals with nanotechnology in improving PCa outcomes will be highlighted., Conclusion: Nanomaterials have great potential, but safety and biocompatibility issues remain. Notwithstanding, the combination of nanomaterials with radiotherapeutics may improve patient outcomes and quality of life., (© 2024. The Author(s).)

Mosquitoes are the most medically important arthropod vectors of several human diseases. These diseases are known to severely incapacitate and debilitate millions of people, resulting in countless loss of lives. Over the years, several measures have been put in place to control the transmission of mosquito-borne diseases, one of which is using repellents. Repellents are one of the most effective personal protective measures against mosquito-borne diseases. However, conventional delivery systems of repellents ( e.g. , creams, gels, and sprays) are plagued with toxicity and short-term efficacy issues. The application of biopolymeric and lipid-based systems has been explored over the years to develop better delivery systems for active pharmaceutical ingredients including mosquito repellents. These delivery systems ( e.g. , solid lipid micro/nanoparticles, micro/nanoemulsions, or liposomes) possess desirable properties such as high biocompatibility, versatility, and controlled/sustained drug delivery, and thus are very important in tackling the clinical challenges of conventional repellent systems. Their capability for controlled/sustained drug release has improved patient compliance as it removes the need for consistent reapplication of repellents. They can also be engineered to reduce repellents' skin permeation, consequently improving their safety. However, despite the benefits that these systems offer very few of them have been successfully translated to the global market for commercial use, a vital challenge that previous reports have not thoroughly examined. The issue of limited clinical translation of novel repellent systems is a vital aspect to consider, as the ultimate goal is to move these systems from bench to bedside. As such, this study seeks to highlight the recent advances in the use of biopolymeric and lipid-based systems for the development of novel mosquito-repellent systems and also analyze the challenges that have limited the clinical translation of these systems while proposing possible strategies to overcome these challenges., Competing Interests: The authors declare no conflict of interest., (This journal is © The Royal Society of Chemistry.)

Prefabricated construction, increasingly recognized as a sustainable method, enhances productivity while mitigating the drawbacks of traditional approaches. Lean construction, pivotal for sustainability, targets waste reduction and cost efficiency while delivering value to customers. In India's prefabrication sector, numerous barriers impede the implementation of lean principles, necessitating their identification and resolution to advance lean practices. This study aims to identify and analyze primary barriers to implementing lean principles within India's prefabrication industry, focusing on professionals' perceptions. Employing exploratory factor analysis, it examines these barriers' interconnections and causal relationships, providing actionable recommendations for enhanced lean construction effectiveness. Through a review of the literature, 26 significant barriers were identified and primary data was obtained with the help of a questionnaire. 25 barriers were discerned after pre-exploratory factor analysis, culminating in ten common components. Notably, the study highlights a primary barrier: understanding of lean construction. Drawing from expert insights, substantial recommendations are provided, intending to guide the prefabricated building sector in overcoming barriers to on-site lean construction. These findings and recommendations offer valuable direction for industry stakeholders., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Author(s).)

Background: The ever-evolving game of soccer is a complex physical contact team sport, exposing its participants to injury., Objectives: To identify the point prevalence of soccer injuries among young amateur, semi-professional, and professional South African male soccer players., Methods: The participation of male amateur (n=54), semiprofessional (n=34), and professional (n=57) players provided a cross-sectional overview of the nature of the most predominant types and anatomical sites of injuries affecting soccer players (average age 23.9±4.7 years). All participants completed the Fuller soccer injury questionnaire, ISAK somatotype profiling and knee flexion/extension isokinetic concentric peak torque (Nm) evaluations at 60°/s., Results: Fifty per cent of the players sustained soccer injuries ( X 2 =0.9). Knee (20%) and ankle (19%) were the most vulnerable sites ( X 2 =0.00001). Knee-injured players' right quadriceps torque (199±37 vs 223±38 Nm) and percentage right quadriceps torque relative to body mass (286±54 vs 311 ±39%) was significantly weaker than the non-injured players (p<0.01). The injured players' right hamstrings/quadriceps (H/Q) torque ratio further significantly differed from the non-injured players' H/Q torque ratios (79±17 vs 70±9%) (p<0.01)., Conclusion: Male soccer players experience neuromusculoskeletal injuries, with their knees and ankles being the most vulnerable. Knee-injured players had weaker quadriceps isokinetic strength than non-injured players., Competing Interests: Conflict of interest and source of funding: The authors declare no conflict of interest and no source of funding.