1. Florfenicol inhibits allergic airway inflammation in mice by p38 MAPK-mediated phosphorylation of GATA 3.
- Author
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Xinxin C, Chi C, Xiao C, Xue X, Yongjun Y, Junqing C, and Xuming D
- Subjects
- Active Transport, Cell Nucleus drug effects, Animals, Asthma enzymology, Bronchoalveolar Lavage Fluid cytology, Cytokines analysis, Dexamethasone pharmacology, Disease Models, Animal, Eosinophils drug effects, Female, Goblet Cells drug effects, Immunoglobulin E analysis, Lung drug effects, Macrophages drug effects, Mice, Mice, Inbred BALB C, Neutrophils drug effects, Phosphorylation, Th2 Cells drug effects, Thiamphenicol therapeutic use, Anti-Bacterial Agents therapeutic use, Anti-Inflammatory Agents, Non-Steroidal therapeutic use, Asthma drug therapy, GATA3 Transcription Factor metabolism, Thiamphenicol analogs & derivatives, p38 Mitogen-Activated Protein Kinases metabolism
- Abstract
Florfenicol has been shown to possess anti-inflammatory activity. However, its possible use for asthma has not yet been studied. First we investigated the anti-inflammatory properties of florfenicol using mice asthma model. BALB/c mice were immunized and challenged by ovalbumin. Treatment with florfenicol caused a marked reduction in inflammatory cells and three Th2 type cytokines in the bronchoalveolar lavage fluids of mice. The levels of ovalbumin-specific IgE and airway hyperresponsiveness were significantly altered after treatment with florfenicol. Histological studies using H&E and AB-PAS staining demonstrate that florfenicol substantially inhibited ovalbumin-induced inflammatory cells infiltration in lung tissue and goblet cell hyperplasia in the airway. These results were similar to those obtained with dexamethasone treatment. We then investigated which signal transduction mechanisms could be implicated in florfenicol activity. Our results suggested that the protective effect of florfenicol was mediated by the inhibition of the p38 MAPK-mediated phosphorylation of GATA 3., (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Published
- 2011
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