Search

Ecuador possesses a great biodiversity and agricultural capacity for the production of table grapes. On the other hand, there is South Korea with a great constant interest in non-traditional fruits. The Ministry of Production, Foreign Trade, Investments, and Fisheries aspires for Ecuador's agri-food exports, including table grapes, to gain presence in the South Korean market. However, if a country intends to introduce its product into an unknown market without a solid action plan, it faces various challenges. The objective of the research is to determine competitive strategies for the export of Ecuadorian grapes through descriptive and comparative studies that allow the entry of table grapes as a non-traditional product into the South Korean market. The methodology used was descriptive, with experimental design. A document study was conducted using quantitative and qualitative data from websites showing trade statistics for the country's international development, such as Trade Map. The results affirm that previously, South Korea has not been interested in non-traditional fruits because there were no agreements or treaties with Ecuador, and it only had the Knowledge Sharing Program (KSP) in place. Therefore, the Strategic Economic Cooperation Agreement (SECA) currently implemented between the two countries is seen as a beneficial competitive strategy tool for Ecuador to gain positioning in the Asian market., Ecuador posee una gran biodiversidad y capacidad agrícola para la producción de la uva de mesa, por otra parte, está Corea del Sur con un gran interés constante en frutas no tradicionales. El Ministerio de Producción Comercio Exterior, Inversiones y Pesca aspira que las exportaciones agroalimentarias del Ecuador incluyendo la uva de mesa, tomen presencia en el mercado de Corea del Sur, sin embargo, si un país pretende introducir su producto en un mercado desconocido y no cuenta con un plan de acción sólida, se enfrenta a diversos desafíos. El objetivo de la investigación es determinar estrategias competitivas para la exportación de uva ecuatoriana mediante estudios descriptivos y comparativos que permitan ingresar la uva de mesa como un producto no tradicional al mercado de Corea del Sur. metodología empleada fue descriptiva, de diseño experimental, se realizó un estudio de documento en donde se utilizaron datos cuantitativos y cualitativos de páginas webs que muestran estadísticas del comercio para el desarrollo internacional del país como Trade Map. En los resultados encontrados se afirma que anteriormente Corea del Sur no ha estado interesado en las frutas no tradicionales por el hecho de que no existían acuerdos o tratados con Ecuador y solo disponía del programa de intercambio de conocimientos KSP. Por ende, es que el Acuerdo Estratégico de Cooperación Económica (SECA) que se ha implementado actualmente entre los dos países, opta como una herramienta de estrategia competitiva beneficiosa para que el Ecuador pueda tomar posicionamiento en el mercado asiático.

To obtain a good harvest, regular pruning of fruit trees and bushes is necessary, which results in the accumulation of piles of cut twigs. These twigs are underutilized and form a large number of agricultural waste. Finding a use for this lignocellulosic biomass is necessary for the sustainable use of resources, as well as for creating additional income for berry growers and rural workers. The purpose of the research was to evaluate the potential of branches of various fruit trees and shrubs as a source of valuable oligomeric polyphenolic compounds – proanthocyanidins, which have a wide range of biologically active properties, including antioxidant, antibacterial, anti-inflammatory, anticancer, etc. Hydrophilic extracts of twigs of sea buckthorn (Hippopae rhamnoides L.), black chokeberry (Aronia melanocarpa), black currant (Ribes nigrum L.), red currant (Ribes rubrum), gooseberry (Grossulariaceae), quince (Cydonia oblonga), raspberry (Rubus L.), and grape (Vitis vinifera) were studied for the first time. The main process for isolating proanthocyanidins from the twigs is the extraction by ethanol-water solutions. The amount of extractive substances in the branches containing proanthocyanidins varied from 6 to 28% per DM. The highest content of proanthocyanidins was found in black chokeberry, quince, and sea buckthorn. The proanthocyanidins isolation from hydrophilic extracts was carried out by Sephadex LH-20. The antimicrobial activity of dominant hydrophilic extracts and purified oligomeric proanthocyanidins was studied against eleven pathogenic bacteria and fungus: Pseudomonas syringae pv. syringae, Erwinia rhapontici, Fusarium culmorum, Verticillium dahlia, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Candida albicans and Cutibacterium acnes. The MIC and MBC/MFC of extracts ranged from 2 to 6 mg/mL. The antimicrobial activity of purified proanthocyanidins was 10 times higher than that of the extracts.

This study explored the elemental, isotopic, and chemometric profiles of Montepulciano d’Abruzzo grapes to ensure the safety and quality of wines produced under the Protected Designation of Origin (PDO) label. Essential nutrients, such as K, P, Ca, Mg, and Fe, were quantified alongside toxic elements, like Hg, Pb, Cd, and As, to evaluate the potential health impacts and compliance with food safety standards. Isotopic analysis provided a powerful tool for geographical authentication, essential for verifying the grapes’ PDO status and preventing market fraud. Chemometric techniques, including Principal Component Analysis (PCA) and Multivariate Classification Methods, were employed to interpret complex datasets, enabling the identification of unique patterns and clusters that signify quality attributes and possible contamination. The study’s findings not only enhance the traceability and authentication processes essential for PDO labels but also offer valuable insights into the safety and quality management of Montepulciano d’Abruzzo grapes, addressing the implications for growers, regulators, and consumers, thereby supporting sustainable viticulture and robust quality control in global wine production., Validerad;2024;Nivå 2;2024-07-24 (signyg);Funder: Sapienza University of Rome (SEED PNR 2021);Fulltext license: CC BY

This study explored the elemental, isotopic, and chemometric profiles of Montepulciano d’Abruzzo grapes to ensure the safety and quality of wines produced under the Protected Designation of Origin (PDO) label. Essential nutrients, such as K, P, Ca, Mg, and Fe, were quantified alongside toxic elements, like Hg, Pb, Cd, and As, to evaluate the potential health impacts and compliance with food safety standards. Isotopic analysis provided a powerful tool for geographical authentication, essential for verifying the grapes’ PDO status and preventing market fraud. Chemometric techniques, including Principal Component Analysis (PCA) and Multivariate Classification Methods, were employed to interpret complex datasets, enabling the identification of unique patterns and clusters that signify quality attributes and possible contamination. The study’s findings not only enhance the traceability and authentication processes essential for PDO labels but also offer valuable insights into the safety and quality management of Montepulciano d’Abruzzo grapes, addressing the implications for growers, regulators, and consumers, thereby supporting sustainable viticulture and robust quality control in global wine production., Validerad;2024;Nivå 2;2024-07-24 (signyg);Funder: Sapienza University of Rome (SEED PNR 2021);Fulltext license: CC BY

Grape is one of the most important and ancient fruits in the world that have been used by humans. the aim of this study was to investigate the effect of post-harvest application of putrescine and spermidine polyamines on maintaining the quantitative and qualitative characteristics of ‘Shahroudi’ grapes during storage. Factorial experiment with two polyamine factors including two types of polyamines, putrescine and spermidine, each in 3 concentrations (0.5, 1 and 2 mmol) and storage time (0, 15, 30, 45, 60, 75, 90 and 105 days) based on a completely randomized design in 2019. After treatment, the fruits were stored for 105 days at a temperature of 2 ° C and a relative humidity of 85-95% and their quantitative and qualitative characteristics were evaluated during storage. The results showed that the treatments had a significant effect on fruit firmness, weight loss, anthocyanin content, titratable acidity, vitamin C and total soluble solids at the level of 1% probability and on pH at the level of 5% probability, but on the percentage of fruit juice It had no significant effect. Also, the effect of storage time on all traits was significant at the 1% probability level. However, the interaction effects of treatment and storage time showed a significant effect on total phenol, acidity and Total soluble solids at 1% probability level and a significant effect on pH at 5% probability level. In conclusion, considering maintain different properties using putrescine and spermidine during storage and delaying in senescence and increasing storage life of fruits of ‘Shahroudi’ grape, polyamines application as a postharvest treatment recommended.

Grape is one of the most important and ancient fruits in the world that have been used by humans. the aim of this study was to investigate the effect of post-harvest application of putrescine and spermidine polyamines on maintaining the quantitative and qualitative characteristics of ‘Shahroudi’ grapes during storage. Factorial experiment with two polyamine factors including two types of polyamines, putrescine and spermidine, each in 3 concentrations (0.5, 1 and 2 mmol) and storage time (0, 15, 30, 45, 60, 75, 90 and 105 days) based on a completely randomized design in 2019. After treatment, the fruits were stored for 105 days at a temperature of 2 ° C and a relative humidity of 85-95% and their quantitative and qualitative characteristics were evaluated during storage. The results showed that the treatments had a significant effect on fruit firmness, weight loss, anthocyanin content, titratable acidity, vitamin C and total soluble solids at the level of 1% probability and on pH at the level of 5% probability, but on the percentage of fruit juice It had no significant effect. Also, the effect of storage time on all traits was significant at the 1% probability level. However, the interaction effects of treatment and storage time showed a significant effect on total phenol, acidity and Total soluble solids at 1% probability level and a significant effect on pH at 5% probability level. In conclusion, considering maintain different properties using putrescine and spermidine during storage and delaying in senescence and increasing storage life of fruits of ‘Shahroudi’ grape, polyamines application as a postharvest treatment recommended.

The sustainability of traditional viticulture systems is compromised by different threats, like those derived from climate change. The use of alternative grapevine varieties is suggested as a possible solution, but different regulations limit this practice in many well-known winemaking regions. In these cases, the exploitation of clonal diversity emerges as a useful alternative of adaptation. Here, 30 ‘Tempranillo Tinto’ clones selected from a grapevine collection of 729 clones initially prospected in the demarcated winemaking region of Rioja (Spain) have been studied. The characterization of 27 traits related to phenology, agronomic performance, and oenological potential during three consecutive seasons revealed a high intra-varietal phenotypic spectrum, of interest to adapt to different growing conditions and needs. Interestingly, this diversity was markedly higher to that observed in a series of commercial clones used as controls. Further analyses revealed their clustering into four groups of clones with significant phenotypic differences. We identified a group of clones with late ripening and long veraison-to-harvest periods, of special interest to respond to projected warming conditions. In general, we proved that old vineyards in traditional winemaking regions are reservoirs of clonal diversity for ancient grapevine varieties like ‘Tempranillo Tinto’, which store useful traits to address current and future viticulture challenges.

Precision viticulture is increasingly being applied to automate and optimize grape production in the vineyard. This paper describes the development of a method for automatic selection of regions of interest from hyperspectral images obtained of a row of vines and intended for prediction of soluble solids content. For this purpose, a dataset consisting of hyperspectral images of a row of ‘Sangiovese’ wine grapes was adopted. Hyperspectral images were acquired directly in the field by means of a hyperspectral imaging Vis/NIR system (400–1000 nm) mounted on a ground-based vehicle. The analyses were carried out on 17 different days, under clear or partly cloudy conditions, in the period between post-veraison and harvest. The vineyard row of Sangiovese vines was divided into 11 sections and a hyperspectral image for each section for each day of analysis was acquired. The regions of interest of the hyperspectral images, comprising the areas representing the grapes, were selected using a PLS-DA-based method. The best PLS-DA model provided excellent results, with sensitivity and specificity values of 0.991 and 0.996, respectively. The mean spectra of the selected regions of interest (ROI) were finally used to predict the soluble solids content (SSC) of the grapes by PLS regression to a primary reference analysis. The results of SSC predictions using the automatic selection of ROIs (R2CV = 0.74 and RMSECV = 0.86 °Brix) were on par with similar regression based on carefully manual selection of ROIs (R2CV = 0.73 and RMSECV = 0.87 °Brix).

Precision viticulture is increasingly being applied to automate and optimize grape production in the vineyard. This paper describes the development of a method for automatic selection of regions of interest from hyperspectral images obtained of a row of vines and intended for prediction of soluble solids content. For this purpose, a dataset consisting of hyperspectral images of a row of ‘Sangiovese’ wine grapes was adopted. Hyperspectral images were acquired directly in the field by means of a hyperspectral imaging Vis/NIR system (400–1000 nm) mounted on a ground-based vehicle. The analyses were carried out on 17 different days, under clear or partly cloudy conditions, in the period between post-veraison and harvest. The vineyard row of Sangiovese vines was divided into 11 sections and a hyperspectral image for each section for each day of analysis was acquired. The regions of interest of the hyperspectral images, comprising the areas representing the grapes, were selected using a PLS-DA-based method. The best PLS-DA model provided excellent results, with sensitivity and specificity values of 0.991 and 0.996, respectively. The mean spectra of the selected regions of interest (ROI) were finally used to predict the soluble solids content (SSC) of the grapes by PLS regression to a primary reference analysis. The results of SSC predictions using the automatic selection of ROIs (R2CV = 0.74 and RMSECV = 0.86 °Brix) were on par with similar regression based on carefully manual selection of ROIs (R2CV = 0.73 and RMSECV = 0.87 °Brix).

Climate change (CC) is a complex phenomenon that has the potential to significantly alter marine, terrestrial, and freshwater ecosystems worldwide. Global warming of 2°C is expected to be exceeded during the 21st century, and the frequency of extreme weather events, including floods, storms, droughts, extreme temperatures, and wildfires, has intensified globally over recent decades, differently affecting areas of the world. How CC may impact multiple food safety hazards is increasingly evident, with mycotoxin contamination in particular gaining in prominence. Research focusing on CC effects on mycotoxin contamination in edible crops has developed considerably throughout the years. Therefore, we conducted a comprehensive literature search to collect available studies in the scientific literature published between 2000 and 2023. The selected papers highlighted how warmer temperatures are enabling the migration, introduction, and mounting abundance of thermophilic and thermotolerant fungal species, including those producing mycotoxins. Certain mycotoxigenic fungal species, such as Aspergillus flavus and Fusarium graminearum, are expected to readily acclimatize to new conditions and could become more aggressive pathogens. Furthermore, abiotic stress factors resulting from CC are expected to weaken the resistance of host crops, rendering them more vulnerable to fungal disease outbreaks. Changed interactions of mycotoxigenic fungi are likewise expected, with the effect of influencing the prevalence and co-occurrence of mycotoxins in the future. Looking ahead, future research should focus on improving predictive modeling, expanding research into different pathosystems, and facilitating the application of effective strategies to mitigate the impact of CC.

By-products from fruit industrialization retain nutritional and functional components; thus, they may find use in animal feeding. This study aimed to assess the effects of dietary fruit industrial by-products on the tilapias blood biochemical and oxidative parameters and on the composition and lipid peroxidation of their fillets. Four diets were supplied to the tilapias: a C-control diet, with no fruit meal, and three diets containing 5% of either acerola (ACM), apple (APM) or grape (GRM) meal. The phenolic compounds and the carotenoids in the meals and their antioxidant capacities were measured. Fish were weighed and measured for the calculation of the growth performance data, their blood was analyzed for health and oxidative status biomarkers and their fillets were analyzed for proximal composition and lipid peroxidation. Grape meal had the highest concentration of phenolics and carotenoids and the highest antioxidant activity, followed by acerola and apple meals. The productive performance was similar among the treatments. The fruit by-product diets either maintained or improved the biochemical biomarkers of health and improved the oxidative status of the fish. The fruit by-product diets increased the concentration of lipids in the fillets and slowed down the onset of the lipid peroxidation during frozen storage., Depto. de Producción Animal, Fac. de Veterinaria, TRUE, pub, Descuento UCM

This studied the effect of method’s and efficiency application of nano- NPK fertilizer (0, 1 and 2. l-1) in addition to the soil or foliar spray on the vegetative growth of Olivetti noier and Thompson Seedless young grapevines grown under Nineveh Governorate during 2021 season. Applications three levels of nano- NPK 0, 2 and 4gm. as soil addition either alone or in combination with foliar application of nano- NPK sprayed three times at 0, 1 and 2.l-1 significantly promoted all vegetative growth traits, height and diameter of the main stem, number of leaves, chlorophyll concentration in leaves, leaf area and leaf area. Moreover, these treatments resulted of vegetative growth parameters compared with untreated (control). Result showed that fertilization with the addition of 4 gm. Nano NPK.l-1, led to a significant increase in the rates of height and diameter of the main stem, number of leaves, chlorophyll concentration in leaves. Foliar fertilization with 2 gm. Nano NPK l-1, led to a significant improving main stem diameter of seedling, leaf area, leaf area of seedling, chlorophyll concentration in leaves. Spray with 1 gm. Nano NPK l-1 led to a significant increase in the height of the main stem and the number of leaves per seedling. Thompson Seedless grape cv. over Olivetti noier cv. in height and diameter of the main stems, leaf area and the leaf area of the seedling.

Polysaccharides are the main group of macromolecules in wines. Climate change is a major problem for viticulturists as it leads to the production of unbalanced grapes. This is attributed to a mismatch between the technological maturity and phenolic maturity of grapes, which can negatively impact the production of high quality wines. To mitigate this effect, biostimulants can be applied to grapevines. For the first time in the literature, this work studied the foliar application of methyl jasmonate plus urea (MeJ + Ur) on the vineyard and its effect on the monosaccharide and polysaccharide composition of Tempranillo grapes and wines over two consecutive seasons. To achieve this, the extraction and precipitation of polysaccharides was conducted, and the identification and quantitation of monosaccharides was performed via GC–MS. The effect of MeJ + Ur foliar treatment in both the grapes and wines was season-dependent. The MeJ + Ur treatment had a slight impact on the monosaccharide composition of the grapes and also demonstrated a small effect on the wines. Multifactor and discriminant analysis revealed that the season had a greater influence on the monosaccharide and polysaccharide composition of grapes and wines compared to the influence of MeJ + Ur treatment. Interestingly, the MeJ + Ur-treated wines exhibited a higher sensory evaluation than the control wines in the second vintage. To gain further insights into the effect of MeJ + Ur foliar application on the monosaccharide and polysaccharide composition of grapes and wines, further investigations should be conducted.

Foliar nutrient sprays are broadly used in commercial agriculture. To date, the application of Ca and Si has been little explored in vineyard production but may lead to positive responses at various levels. Therefore, the aim of this work was to evaluate the effect of supplying single or combined calcium (Ca, 120 mM) and silicon (Si, 120 mM) sprays in Tempranillo grape composition. Foliar treatment with Ca + Si foliar enhanced all families of aromatic compounds, whereas single Ca and Si sprays induced lower effects. Regarding phenolic compounds, all foliar treatments led to minor effects. However, all three foliar Ca and Si treatments increased the total grape amino acid content. Consequently, the application of combined Ca and Si sprays to a vineyard is recommended as a tool for improving grape quality.

The disruptive effect exerted by high-power ultrasound (US) on grape cell walls is established as the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines. Given the biochemical differences that exist between the cell walls of different grape varieties, this paper investigates whether the effect of the application of US in a winery may vary according to the grape variety treated. Wines were elaborated with Monastrell, Syrah and Cabernet Sauvignon grapes, applying a sonication treatment to the crushed grapes using industrial-scale equipment. The results showed a clear varietal effect. The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides. These findings correlate with the differences in the composition and structure of their cell walls since those of Monastrell grapes presented biochemical characteristics associated with a greater rigidity and firmness of the structures.

The Fondillón wine from Alicante is considered and recognised in the European market, together with other wines of recognised prestige, such as Porto, Sherry or Bordeaux, as a luxury wine. In this paper we are going to analyse the most important aspects concerning this wine product, based on the analysis of the protected plant variety Monastrell grape from the data extracted from the CPVO database and the regulation established in the Regulations and Specifications of the Regulatory Council of the PDO Wines of Alicante, carrying out an analysis of the main socio-economic purposes involved in the establishment of a protected designation of origin. In addition, we will make a proposal for the legal regulation of those sweet wines similar to Fondillón, but which do not meet any of the requirements to be recognised by the traditional term, based on the combination of two figures that make up the legal regime of quality signs: varietal wines and certification marks registered in the EUIPO.

Phenolic compounds are very important for grapes and wines quality since these compounds can take part in color, mouthfeel and wine ageing potential, and they are also related to the human healthy properties of moderate wine consumption. The content of phenolic compounds in grapes depends on different factors, such as variety, climatic and geographical factors, cultural practices, and the stage of grape ripeness. Foliar application of biostimulants has been studied in the last years in order to mitigate the effect of climatic change in grapes and to enhance their composition and quality. In this work, it was studied the effect of foliar application of methyl jasmonate plus urea (MeJ + Ur), in Tempranillo vineyard, on the content of phenolic compounds in grapes and wines, during two consecutive vintages. These compounds (anthocyanins, flavanols, flavonols, phenolic acids, and stilbenes) were analysed, in both grapes and wines, by high performance liquid chromatography (HPLC). The effect of foliar treatment was season dependent, probably due to the differences in pre-harvest rainfall recorded. In the first season, the MeJ + Ur treatment favored the biosynthesis of anthocyanins, increasing their content in grapes; however, this effect was not observed in the wines. In the second vintage, the MeJ + Ur foliar application did not enhance the anthocyanin content in grapes or in wines. With respect to the other phenolic compounds, the foliar treatment did not improve their content in grapes, except for total flavonols in the second vintage. Foliar application of MeJ + Ur seems to be a good tool in order to enhance the anthocyanin content in grapes, which it is very interesting due to anthocyanins are the main phenolic compounds in red varieties, and they are responsible for the wine red color. Nevertheless, further studies should be carried out to achieve a better knowledge of the plant response to this treatment according to the climatic conditions. Moreover, it is necessa

The typical phenolic profile in grapes is characterized by its complexity both in terms of number of diverse chemical structures and their variation during ripening. Besides, the specific phenolic composition of grapes directly influences the presence of those components in the resulting wine. In this contribution, a new method based on the application of comprehensive two-dimensional liquid chromatography coupled to a diode array detector and tandem mass spectrometry has been developed to obtain the typical phenolic profile of Malbec grapes cultivated in Brazil. Moreover, the method has been demonstrated to be useful to study how the phenolic composition in grapes evolved during a 10-week ripening period. Main detected compounds in grapes and in the wine derived from them were anthocyanins, although a good number of polymeric flavan-3-ols were also tentatively identified, among other compounds. Results show how the amount of anthocyanins present in grapes was increased during ripening up to 5–6 weeks and then decreased towards week 9. The two-dimensional approach applied was demonstrated to be useful for the characterization of the complex phenolic profile of these samples, involving more than 40 different structures and has the potential to be further applied to the study of this important fraction is different grapes and wines systematically.

Specific indicators of environmental conditions and drying properties were analyzed using two grape cultivars (‘Crimson’ and ‘Thompson’), artificially inoculated with Aspergillus carbonarius under open-air and tunnel drying conditions. Air temperature (T), relative humidity (RH), grape surface temperature (Ts) and water activity (aw) throughout the drying experiment, grape moisture content, fungal colonisation and OTA contamination during the drying process and their interactions were analyzed. Drying properties such as effective water diffusivity (Deff) and water transfer resistant of the peel were estimated. Ts was 5-7°C higher in the tunnel than in the open-air; the infected grapes had a higher maximum Ts than the control (around 4-6°C). OTA contamination was higher in the tunnel than in the open-air-dried grapes, but fungal colonies showed the opposite trend. Water diffusivity (Deff) was 54% higher in the tunnel than in the open-air; infected grapes had more than 70% higher Deff than control grapes, differences explained by factors affecting the water transport. This study highlighted CFU and OTA as indicators affecting the water availability between red and white grapes during open-air and tunnel drying, as estimated by Deff and peel resistant.

Hypertension (HTN) is one of the leading causes of death in the world. Agri-food by-products are emerging as a novel source of natural antihypertensive agents allowing for their valorization and making food and agricultural industries more environmentally friendly. In this regard, wine making process generates large amounts of by-products rich in phenolic compounds that have shown potential to exert several beneficial effects including antihypertensive properties. The aim of this study was to review the blood pressure-lowering effects of winery by-products. In addition, molecular mechanisms involved in their bioactivity were also evaluated. Among the winery by-products, grape seed extracts have widely shown antihypertensive properties in both animal and human studies. Moreover, recent evidence suggests that grape stem, skin and pomace and wine lees may also have great potential to manage HTN, although more studies are needed in order to confirm their potential in humans. Improvement of endothelial dysfunction and reduction of oxidative stress associated with HTN are the main mechanisms involved in the blood pressure-lowering effects of these by-products.

Climate change is posing a major challenge to the wine industry, with rising alcohol levels emerging as an issue of concern affecting quality, economics and health. This study explores two methods to reduce alcohol content in Chardonnay wines from Chile. Firstly, 5% and 10% of water was added to grape must. Secondly, the sequential inoculation of Metschnikowia pulcherrima with Saccharomyces cerevisiae was examined. The main objectives were to assess the efficacy of these treatments in reducing alcohol levels and their impact on organoleptic properties. Our findings revealed that the presence of M. pulcherrima in winery conditions was less effective in reducing ethanol. Nevertheless, wines resulting from this treatment exhibited an interesting composition with distinct sensory profiles. Furthermore, the Sc-5% W condition displayed promising results by reducing ethanol content by 0.47% (v/v), with less significant changes in the sensory profile. Although the Sc-10% W wines showed a more substantial ethanol reduction of 1.73% (v/v), they exhibited a decreasing trend in volatile compounds and polysaccharides, ultimately being perceived as less complex in sensory analysis and not being preferred by consumers. This research contributes to understanding how these approaches affect the alcohol content and sensory attributes of white wines and is fundamental to the sustainability of the sector and the ability of the sector to recover from climate challenges.

This review describes the process of creation and consolidation of the Instituto de la Uva, of Universidad Centroccidental Lisandro Alvarado (UCLA) which includes historical passages since its foundation and brief comments on its research, teaching, extension and production activities. Mention is made of its academic staff and experimental fields. The review ends by pointing out the current situation of the Institute, in which much of its surface, infrastructure and equipment has been invaded and looted by unscrupulous people, Esta resenha descreve o processo de criação e consolidação do Instituto de la Uva, da Universidad Centroccidental Lisandro Alvarado (UCLA) que inclui passagens históricas desde sua fundação e breves comentários sobre suas atividades de pesquisa, ensino, extensão e produção. Menciona-se o seu corpo docente e campos experimentais. A resenha termina apontando a situação atual do Instituto, em que grande parte de sua superfície, infraestrutura e equipamentos foram invadidos e saqueados por pessoas sem escrúpulos, Se describe el proceso de creación y consolidación del Instituto de la Uva, de la Universidad Centroccidental Lisandro Alvarado (UCLA) la cual incluye pasajes históricos desde su fundación y breves comentarios sobre su actividad de investigación, docencia, extensión y producción. Se hace mención a su personal académico y los campos experimentales. La reseña culmina señalando la situación actual del Instituto, en la que mucha de su superficie, infraestructura y equipos ha sido invadida y saqueada por personas inescrupulosas

The combination of computer vision devices such as multispectral cameras coupled with artificial intelligence has provided a major leap forward in image-based analysis of biological processes. Supervised artificial intelligence algorithms require large ground truth image datasets for model training, which allows to validate or refute research hypotheses and to carry out comparisons between models. However, public datasets of images are scarce and ground truth images are surprisingly few considering the numbers required for training algorithms.

Wine quality is determined by the local terroir, nature of grapevines, viticultural practices and vinification processes. Scientists have identified chemical compounds, typically volatiles, that can give desirable sensational attributes during wine tasting and potentially become chemical markers of high-quality wine. However, there is a lack of convincing statistical models using grape volatile composition to predict the resulting wine quality. Besides, there are still volatiles and other factors such as variations in biological factors of consumers to be revealed. Overall, this PhD thesis examines the influences of grape and wine volatiles on wine quality classification and perception during wine tasting.

Multiple grape powdery mildew (PM) genetic resistance (R) loci have been found in wild grape species. Little is known about the defense responses associated with each R locus. In this study, we compare the defense mechanisms associated with PM resistance in interspecific crosses segregating for a single R locus from Muscadinia rotundifolia (Run1, Run1.2b, Run2.1, Run2.2), Vitis cinerea (Ren2), V. romanetii (Ren4D and Ren4U), and the interspecific hybrid Villard blanc (Ren3). By combining optical microscopy, visual scoring, and biomass estimation, we show that the eight R loci confer resistance by limiting infection at different stages. We assessed the defense mechanisms triggered in response to PM at 1 and 5 days post-inoculation (dpi) via RNA sequencing. To account for the genetic differences between species, we developed for each accession a diploid synthetic reference transcriptome by incorporating into the PN40024 reference homozygous and heterozygous sequence variants and de novo assembled transcripts. Most of the R loci exhibited a higher number of differentially expressed genes (DEGs) associated with PM resistance at 1 dpi compared to 5 dpi, suggesting that PM resistance is mostly associated with an early transcriptional reprogramming. Comparison of the PM resistance-associated DEGs showed a limited overlap between pairs of R loci, and nearly half of the DEGs were specific to a single R locus. The largest overlap of PM resistance-associated DEGs was found between Ren3 +, Ren4D +, and Ren4U + genotypes at 1 dpi, and between Ren4U + and Run1 + accessions at 5 dpi. The Ren3 +, Ren4D +, and Ren4U + were also found to have the highest number of R locus-specific DEGs in response to PM. Both shared and R locus-specific DEGs included genes from different defense-related categories, indicating that the presence of E. necator triggered distinct transcriptional responses in the eight R loci.

BACKGROUND AND AIMS: Although plastid genes are widely used in phylogenetic studies, signals of positive selection have been scarcely investigated in the grape family. The plastomes from 91 accessions of Vitaceae were examined to understand the extent to which positive selection is present and to identify which genes are involved. Moreover, the changes through time of genes under episodic positive selection were investigated and the hypothesis of an adaptive process following the Cretaceous-Palaeogene (K/Pg) transition about 66 million years ago was tested. METHODS: Different codon-substitution models were used to assess pervasive and episodic positive selection events on 70 candidate plastid genes. Divergence times between lineages were estimated and stochastic character mapping analysis was used to simulate variation over time of the genes found to be under episodic positive selection. KEY RESULTS: A total of 20 plastid genes (29 %) showed positive selection. Among them, 14 genes showed pervasive signatures of positive selection and nine genes showed episodic signatures of positive selection. In particular, four of the nine genes (psbK, rpl20, rpoB, rps11) exhibited a similar pattern showing an increase in the rate of variation close to the K/Pg transition. CONCLUSION: Multiple analyses have shown that the grape family has experienced ancient and recent positive selection events and that the targeted genes are involved in essential functions such as photosynthesis, self-replication and metabolism. Our results are consistent with the idea that the K/Pg transition has favoured an increased rate of change in some genes. Intense environmental perturbations have influenced the rapid diversification of certain lineages, and new mutations arising on some plastid genes may have been fixed by natural selection over the course of many generations.

Grapes are one of the richest sources of polyphenols in the Mediterranean diet and, therefore, a very good source of antioxidants in the human diet. For practical reasons, in recent years the market for seedless grape varieties has grown exponentially. These varieties are not well characterized yet, and therefore it is necessary to study the changes in composition that these new varieties bring in. Likewise, the effect of digestion on the bioavailability of polyphenols in foods of plant origin is well known, which, consequently, will also affect antioxidant activity and, in general, bioactivity. In this work, we studied the effect of the grape variety on the initial grape composition and on the absorbable fraction, as it would reach the intestine after in vitro digestion. Our results showed that black and red varieties have great potential for increasing the antioxidant content of the diet. Additionally, we have concluded that all polyphenols, with the exception of flavanols, see their bioavailable fraction diminished under in vitro conditions.

Grape volatile compounds determine the wine quality and typicity. The aim of this work was to study the effects of several foliar applications in 'Garnacha', 'Tempranillo', and 'Graciano' grapevines in order to enhance their grape volatile composition. The field trial involved the application of two nitrogen compounds, urea (Ur) and phenylalanine (Phe), and two elicitors, methyl jasmonate (MeJ) and a yeast extract (YE). The analysis of grape volatile compounds was carried out by HS-SPME-GC-MS. For 'Garnacha', most terpenes, and C13 norisoprenoids increased their grape content by applying Ur and Phe, and especially MeJ. For 'Tempranillo', treatments with Ur and MeJ improved the synthesis of most terpenoids. For 'Graciano', a trend to decrease the terpenoids content in grapes with the treatments was observed; however, Phe application increased C13 norisoprenoids content. In conclusion, foliar application of, Phe or Ur, and an elicitor, MeJ, can be a useful tool to improve grape quality.

This project aims to improve the management of late season bunch rots of grape (LSBR) whichcan be caused by a wide range of fungal pathogens. LSBR collectively have been an increasing issue in Mid-Atlantic vineyards, severely affecting grape yield and quality. Despite intensive fungicide spray programs and cultural practices, severe LSBR epidemics threaten the budding Mid-Atlantic wine industry. The basic plant pathological variables of host, pathogen, and environment were investigated to improve knowledge of the diseases involved, and therefore improve management strategies. The most common causal agents of LSBR in the Mid-Atlantic were found to be Botrytis cinerea and Colletotrichum spp. and the species identity of less common fungi was also investigated. The next most prevalent fungi associated with LSBR, Alternaria alternata, Aspergillus uvarum, and Neopestalotiopsis rosae were evaluated for pathogenicity in field experiments through the artificial inoculation of grape clusters. Second, the sensitivity of A. uvarum, B. cinerea, and N. rosae to commonly used chemical classes of fungicides was tested. Lastly, the optimal infection conditions and timing for Colletotrichum spp. were evaluated in laboratory, field, and greenhouse experiments, resulting in a quantitative inoculum tracking technique and a disease prediction model. These experiments were focused on solving practical and important disease management issues experienced by local grape growers, while conducting novel research that was applicable to the broader science community. Beyond the increased knowledge of the etiology and epidemiology of LSBR, the conclusions of this research could lead to reformed LSBR management strategies with the elimination of unnecessary and ineffective fungicide applications, increased accuracy and timing of management efforts, and increased marketable grape yield.

Proton nuclear magnetic resonance (1H NMR) metabolomics was employed to investigate the impact of water deficit, defoliation, and crop thinning on the chemical composition of must and wines from the cool-climate white grape variety Solaris. The obtained results show that viticultural practices (defoliation and crop thinning) affected the amino acid and sugar content of Solaris must and thereby the quality of the final wine-mainly in terms of compounds normally related to fruity aroma (i.e., isopentanol), non-sugar sweetness (i.e., proline and glycerol), and alcohol content. The content of tyrosol, a natural phenolic antioxidant with a high bioavailability, was increased in the final wine by a combination of defoliation and crop thinning. The results of the metabolomics analysis performed on the must and wine samples from the water stress experiment showed that short-term water deficit significantly affected the concentration of several flavor-related compounds, including glutamate, butyrate and propanol, of the organic acids lactate and fumarate, and of the phenolic compounds caffeic acid and p-coumaric acid. ANOVA simultaneous component analysis showed that the effect of water deficit accounted for 11% (p < 0.001) and 8% (p < 0.001) of the variability in the metabolite concentrations in must and wines, respectively, while viticultural practices accounted for 38% (p < 0.001) and 30% (p < 0.001) of the metabolite variability in must and wines, respectively.

Proton nuclear magnetic resonance (1H NMR) metabolomics was employed to investigate the impact of water deficit, defoliation, and crop thinning on the chemical composition of must and wines from the cool-climate white grape variety Solaris. The obtained results show that viticultural practices (defoliation and crop thinning) affected the amino acid and sugar content of Solaris must and thereby the quality of the final wine-mainly in terms of compounds normally related to fruity aroma (i.e., isopentanol), non-sugar sweetness (i.e., proline and glycerol), and alcohol content. The content of tyrosol, a natural phenolic antioxidant with a high bioavailability, was increased in the final wine by a combination of defoliation and crop thinning. The results of the metabolomics analysis performed on the must and wine samples from the water stress experiment showed that short-term water deficit significantly affected the concentration of several flavor-related compounds, including glutamate, butyrate and propanol, of the organic acids lactate and fumarate, and of the phenolic compounds caffeic acid and p-coumaric acid. ANOVA simultaneous component analysis showed that the effect of water deficit accounted for 11% (p < 0.001) and 8% (p < 0.001) of the variability in the metabolite concentrations in must and wines, respectively, while viticultural practices accounted for 38% (p < 0.001) and 30% (p < 0.001) of the metabolite variability in must and wines, respectively.

Grape (Vitis vinifera L.) is one of the most popular fruits worldwide. It contains various bioactive compounds, such as proanthocyanidins, anthocyanins, flavonols, phenolic acids and stilbenes, the contents of which could vary considerably in grape skin, pulp and seed. Many studies have revealed that grape possesses a variety of health benefits, such as antioxidant, anti-inflammatory, gut-microbiota-modulating, anticancer and cardioprotective effects. Grape is eaten as fresh fruit and is also used as raw material to produce various products, such as wine, grape juice and raisins. Moreover, the byproducts of grape, such as grape pomace and grape seed, have many applications in the food industry. In this paper, the bioactive compounds in grape are briefly summarized based on literature published in recent years. In addition, the health benefits of grape and its bioactive components are discussed, with special attention paid to the underlying mechanisms. Furthermore, the applications of grape in the food industry are elucidated, especially the applications of grape pomace and grape seed. This paper can contribute to understanding the health benefits and mechanisms of grape and its bioactive compounds, as well as the promotion of the use of grape in the food industry. © 2022 by the authors.

Fruit and vegetables are excellent sources of health-promoting bioactive compounds and nutraceuticals. Regular consumption of fruit and vegetables helps prevent the onset and progression of many non-communicable diseases. The Mediterranean diet envisages consumption of healthy vegetables and fruit on a daily basis for maximum health benefits. Traditional use envisages vegetable-based and fruit-based diets, and many studies scientifically proved the beneficial effects of Mediterranean vegetables and fruits. Rich in bioactive phytochemicals, citrus, cucumbers and grapes have antioxidant, anti-inflammatory, antimicrobial, cardioprotective, anti-ageing and anti-cancer properties. Studies indicate that intake of citrus, cucumbers and grapes reduces hypertension, hyperlipidemia, skin problems and infections and improves the health of the cardiovascular and nervous systems. These beneficial effects are mediated by several bioactive molecules present in Mediterranean diet vegetables and fruits, such as citrus, cucumbers and grapes. Indeed, they contains flavones, isoflavones, tannins, polyphenols and many beneficial natural molecules. This review focuses on the bioactive ingredients in citrus fruit, cucumbers and grapes, all components of the Mediterranean diet, and their health effects. A deep understanding of Mediterranean diet’s components, as well as clinical trials to test natural molecules beneficial effects, will permit to further explore the therapeutic potential of the Mediterranean diet in several pathological conditions.

Borax detection can be done using the flame test method, volumetric titration, spectrophotometric analysis, and qualitatively using natural materials such as curcumin and anthocyanins. The high content of anthocyanins in grapes has the potential to be utilized in acid-base titrations as an indicator of natural acid-base. The research objective was to determine the potential of anthocyanins in the flesh and skin of grapes in detecting natural borax which is absorbed on filter paper, Whatmann paper no. 41 and Whatmann paper no. 42. Then it was tested on samples of meatballs from 5 sellers in Samarinda Seberang to Mangkupalas village. The research method used is a quantitative analysis of the color changes that occur. Based on the research results, filter paper can have a better absorption that is shown with a dark color on Whatmann No. 41 and Whatmann No.42. Grape skin extract can detect borax more clearly than flesh by forming a dark purple ring when reacting with borax. In testing using meatball samples, the overall results showed that the samples contained borax. It was concluded that the filter paper from the absorption of grape skin anthocyanins could be used as an alternative in quantitatively testing the borax content in food., Deteksi boraks dapat dilakukan dengan menggunakan metode uji nyala api, titrasi volumetrik, analisis spektrofotometri serta secara kualitatif menggunakan bahan alam seperti kurkumin dan antosianin. Kandungan antosianin di dalam anggur yang cukup tinggi memiliki potensi pemanfaatan dalam titrasi asam basa sebagai indikator asam basa alami. Tujuan penelitian yaitu untuk mengetahui potensi antosianin pada daging dan kulit buah anggur dalam mendeteksi boraks secara alami yang diabsorpsikan pada kertas saring biasa, kertas Whatmann no. 41 dan kertas Whatmann no.42. Kemudian diujicobakan pada sampel pentol bakso dari 5 penjual yang berada di sekitar Samarinda Seberang hingga ke Mangkupalas. Metode penelitian yang digunakan adalah analisis secara kuantitatif perubahan warna yang terjadi. Berdasarkan hasil penelitian kertas saring bisa memiliki daya absorbsi yang lebih baik ditandai dengan warna yang pekat dibandingkan Whatmann no. 41 dan Whatmann no.42. Ekstrak kulit anggur dapat mendeteksi boraks lebih jelas dibandingkan dengan buah anggurnya dengan terbentuknya cincin berwarna ungu tua jika direaksikan dengan boraks. Pada pengujian menggunakan sampel pentol bakso dari 5 penjual, diperoleh hasil keseluruhan sampel menggandung boraks. Disimpulkan bahwa kertas saring dari absorbsi antosianin kulit anggur dapat digunakan sebagai alternatif dalam menguji kandungan boraks pada makanan secara kuantitatif.

Borax detection can be done using the flame test method, volumetric titration, spectrophotometric analysis, and qualitatively using natural materials such as curcumin and anthocyanins. The high content of anthocyanins in grapes has the potential to be utilized in acid-base titrations as an indicator of natural acid-base. The research objective was to determine the potential of anthocyanins in the flesh and skin of grapes in detecting natural borax which is absorbed on filter paper, Whatmann paper no. 41 and Whatmann paper no. 42. Then it was tested on samples of meatballs from 5 sellers in Samarinda Seberang to Mangkupalas village. The research method used is a quantitative analysis of the color changes that occur. Based on the research results, filter paper can have a better absorption that is shown with a dark color on Whatmann No. 41 and Whatmann No.42. Grape skin extract can detect borax more clearly than flesh by forming a dark purple ring when reacting with borax. In testing using meatball samples, the overall results showed that the samples contained borax. It was concluded that the filter paper from the absorption of grape skin anthocyanins could be used as an alternative in quantitatively testing the borax content in food., Deteksi boraks dapat dilakukan dengan menggunakan metode uji nyala api, titrasi volumetrik, analisis spektrofotometri serta secara kualitatif menggunakan bahan alam seperti kurkumin dan antosianin. Kandungan antosianin di dalam anggur yang cukup tinggi memiliki potensi pemanfaatan dalam titrasi asam basa sebagai indikator asam basa alami. Tujuan penelitian yaitu untuk mengetahui potensi antosianin pada daging dan kulit buah anggur dalam mendeteksi boraks secara alami yang diabsorpsikan pada kertas saring biasa, kertas Whatmann no. 41 dan kertas Whatmann no.42. Kemudian diujicobakan pada sampel pentol bakso dari 5 penjual yang berada di sekitar Samarinda Seberang hingga ke Mangkupalas. Metode penelitian yang digunakan adalah analisis secara kuantitatif perubahan warna yang terjadi. Berdasarkan hasil penelitian kertas saring bisa memiliki daya absorbsi yang lebih baik ditandai dengan warna yang pekat dibandingkan Whatmann no. 41 dan Whatmann no.42. Ekstrak kulit anggur dapat mendeteksi boraks lebih jelas dibandingkan dengan buah anggurnya dengan terbentuknya cincin berwarna ungu tua jika direaksikan dengan boraks. Pada pengujian menggunakan sampel pentol bakso dari 5 penjual, diperoleh hasil keseluruhan sampel menggandung boraks. Disimpulkan bahwa kertas saring dari absorbsi antosianin kulit anggur dapat digunakan sebagai alternatif dalam menguji kandungan boraks pada makanan secara kuantitatif.

This review describes the process of creation and consolidation of the Instituto de la Uva, of Universidad Centroccidental Lisandro Alvarado (UCLA) which includes historical passages since its foundation and brief comments on its research, teaching, extension and production activities. Mention is made of its academic staff and experimental fields. The review ends by pointing out the current situation of the Institute, in which much of its surface, infrastructure and equipment has been invaded and looted by unscrupulous people, Esta resenha descreve o processo de criação e consolidação do Instituto de la Uva, da Universidad Centroccidental Lisandro Alvarado (UCLA) que inclui passagens históricas desde sua fundação e breves comentários sobre suas atividades de pesquisa, ensino, extensão e produção. Menciona-se o seu corpo docente e campos experimentais. A resenha termina apontando a situação atual do Instituto, em que grande parte de sua superfície, infraestrutura e equipamentos foram invadidos e saqueados por pessoas sem escrúpulos, Se describe el proceso de creación y consolidación del Instituto de la Uva, de la Universidad Centroccidental Lisandro Alvarado (UCLA) la cual incluye pasajes históricos desde su fundación y breves comentarios sobre su actividad de investigación, docencia, extensión y producción. Se hace mención a su personal académico y los campos experimentales. La reseña culmina señalando la situación actual del Instituto, en la que mucha de su superficie, infraestructura y equipos ha sido invadida y saqueada por personas inescrupulosas

The aim of this paper is to develop a synthetic model reproducing more realistically the conditions of grape juice to study browning caused by laccase from Botrytis cinerea. The laccase browning kinetics were measured by monitoring the increase in absorbance at 420 nm over time in the presence of different substrates – one monophenol: 4-hydroxybenzoic acid; three orthodiphenols: caftaric acid, (+)-catechin and (?)-epicatechin; and one triphenol: gallic acid. The results indicate that orthodiphenols are better substrates than triphenols and that monophenols do not appear to be reactive. Of the orthodiphenols, (+)-catechin showed the greatest browning intensity, followed in decreasing order by (?)-epicatechin and caftaric acid. These results confirm that sulfur dioxide, ascorbic acid and glutathione really do protect grape juice against laccase browning. The effectiveness of ascorbic acid and glutathione also confirm that both antioxidants can be useful tools for reducing doses of sulfur dioxide in winemaking, especially when grey rot is present. © 2021 The Authors

Grape cultivation is a billion-dollar agricultural sector in California, where invasive or novel pest species can disrupt management practices. We report herein on a new pest associated with California vineyards, the carpentermoth Givira ethela (Neumoegen and Dyar, 1893). Rather than an invasive species, G. ethela appears to be a newly recognized wood-boring pest of Vitis vinifera (L.) in regions of Californias Central Valley, where its initial occurrence has been dated back to, at least, the beginning of the 2000s. The habitus of adult, genitalia and pupa is illustrated. Givira ethela distribution in California is updated including published records and new data. Carpentermoth galleries seem to facilitate the access of Planococcus ficus Signoret, 1875 to vine sap and protection from natural enemies, environmental stresses, and pesticide treatments. Notes on pest status, life history, monitoring practices, natural enemies, and management options on grapes are also discussed. Tools for the Integrated Pest Management of G. ethela should include the correct identification of the insect and its damage, a full understanding of its biology and ecology, the application of monitoring methods, and the identification of economic thresholds and injury levels.

[EN] Climate change scenarios are predicting an increase in temperature as well as more scarce and torrential rainfall episodes. Due to this, an imbalance between grape technological and phenolic maturity is being observed detrimentally affecting grapes composition. In semi-arid areas, irrigation management is a main field practice to influence grape ripening. The goal of the present study was to investigate in Vitis vinifera L. cv. Bobal grapevine responses to three watering regimes: i) Rainfed, ii) deficit irrigation (DI) replacing only 35% of the estimated crop evapotranspiration (ETc) and ii) full irrigation (FI) replacing 100% ETc. In the mid-summer, rainfed grapevines showed different degrees of water stress determined by midday stem water potentials (¿stem) ranging from -1.1 to -1.4 MPa depening on the season. Rainfed plants had in all seasons less vigor and production and, at harvest, higher concentrations of total soluble solids (TSS) and grape phenolics compounds, as well as lower pH, with respect to the other water regimes studied. DI grapevines, generally, had intermediate values between Rainfed and FI, which presented extreme values of the studied parameters respect to Rainfed. The effects observed on grape color parameters and phenolic compounds with the Rainfed regime were mainly due to a dehydration of the berry, which lowered the yield and the weight of the berry compared to the irrigated treatments. The lower TSS accumulation in the DI berries with respect to the Rainfed, will favour obtaining wines with lower alcohol content, currently more demanded by the consumers. Besides, despite the differences obtained between water regime treatments in the TSS accumulation, the extractability of the anthocyanins was similar, which is interesting since anthocyanin extraction from grapes is prerequisite to the formation of stable red wine pigments. Although the most convenient irrigation strategy might depend to the wine style to be obtained, DI is a strategy

In this study, we have analyzed the anthocyanin composition of skin extracts of three red grape varieties Prokupac, Evita and Čokot Zemun in order to distinguish these cultivars based on their anthocyanin profile. Also, mechanical analysis of grape bunches and berries was performed. According to our results, seventeen anthocyanins were identified using LC/MS technique and quantitative differences were recorded using HPLC-DAD method. The highest content of total anthocyanins was obtained for Evita variety and the lowest one was recorded in Prokupac. Also, clear differences were observed in anthocyanins ratios. In comparison to Prokupac and Evita varieties, Čokot Zemun was characterized with a high content of coumaroyl derivatives of anthocyanin compounds, while high levels of acetylated derivatives were recorded in Prokupac. Data reported in this study represent a certain contribution to a database of mechanical properties and chemical composition of grape varieties originating from Balkan.

Exercise induces free radicals' overproduction and therefore, an enhancement of oxidative stress, defined as an imbalance between the production of reactive species and the intrinsic antioxidant defense. Redox activity of reactive species plays an important and a positive role on exercise adaptation, but these species at very high concentrations have detrimental effects. As a result, the use of antioxidant supplements for reducing oxidative stress can be an effective health strategy to maintain an optimal antioxidant status. In this sense, grapes are an important source of natural antioxidants due to their high content in polyphenols. They have shown antioxidant potential benefits for the reduction of intense exercise effect in athletes of different sport disciplines. Consequently, it is plausible to hypothesize that a strategic supplementation with grape based products may be a good approach to mitigate the exercise induced oxidative stress. The goal of this review is to present the state of the art of supplementation effects with grape beverages and grape extracts on the oxidative stress markers in athletes. The data of polyphenolic dosages, participant characteristics and exercise protocols are reported.

In this study we have analyzed the anthocyanin composition of skin extracts of three red grape varieties Prokupac, Evita and Čokot Zemun in order to distinguish these cultivars based on their anthocyanin profile. Also, mechanical analysis of grape bunches and berries was performed. According to our results, seventeen anthocyanins were identified using LC‐MS technique and quantitative differences were recorded using HPLC‐DAD method. The highest content of total anthocyanins was obtained for Evita variety and the lowest one was recorded in Prokupac. Also, clear differences were observed in anthocyanins ratios. In comparison to Prokupac and Evita varieties, Čokot Zemun was characterized with a high content of cumaroyl derivatives of anthocyanin compounds, while high levels of acetylated derivatives were recorded in Prokupac. Data reported in this study represent a certain contribution to a database of mechanical properties and chemical composition of grape varieties originating from Balkan.

Exercise induces free radicals' overproduction and therefore, an enhancement of oxidative stress, defined as an imbalance between the production of reactive species and the intrinsic antioxidant defense. Redox activity of reactive species plays an important and a positive role on exercise adaptation, but these species at very high concentrations have detrimental effects. As a result, the use of antioxidant supplements for reducing oxidative stress can be an effective health strategy to maintain an optimal antioxidant status. In this sense, grapes are an important source of natural antioxidants due to their high content in polyphenols. They have shown antioxidant potential benefits for the reduction of intense exercise effect in athletes of different sport disciplines. Consequently, it is plausible to hypothesize that a strategic supplementation with grape based products may be a good approach to mitigate the exercise induced oxidative stress. The goal of this review is to present the state of the art of supplementation effects with grape beverages and grape extracts on the oxidative stress markers in athletes. The data of polyphenolic dosages, participant characteristics and exercise protocols are reported.

In the present work, a paste was developed from pomace and amaranth flour, which was obtained according to the methodology of Morato (2009), and later, it was mixed with amaranth in different concentrations to generate three formulations (50:50, 55:45,60:40) called M1, M2 and M3. M3 (Experimental) was the one that presented the highest levels of protein, fat, sugar, fiber and antioxidant content when compared to a commercial pasta (Yemina®) type spaghetti. The main results were a high content of protein and fiber (17.56 and 37.16 g/100 g), respectively. In the case of phenols, flavonoids and tannins, they were up to ten times higher compared to commercial pasta. The paste with pomace will be a new product, which if accepted sensory could give added value to the pomace and to the grape producers of the state of Queretaro., En el presente trabajo se desarrolló una pasta a partir de harina de orujo y amaranto, la cual se obtuvo de acuerdo con la metodología de Morato (2009), se mezcló con amaranto en diferentes concentraciones para generar tres formulaciones (50:50, 55:45, 60:40) denominadas M1, M2 y M3. La M3 (Experimental) fue la que presentó los niveles más altos en contenido de proteínas, grasas, azúcares, fibra y antioxidantes al compararla con una pasta comercial (Yemina®) tipo espagueti. Los principales resultadosfueron un alto contenido en proteína y fibra (17.56 y 37.16 g/100 g), respectivamente. En el caso de los fenoles, flavonoides y taninos fueron superiores hasta 10 veces comparados con la pasta comercial. La pasta con orujo será un producto nuevo, que de ser aceptado sensorialmente podría dar un valor agregado al orujo y a los productores de uva del estado de Querétaro.

Tartaric acid (TA) is an obscure end point to the catabolism of ascorbic acid (Asc). Here, it is proposed as a “specialized primary metabolite”, originating from carbohydrate metabolism but with restricted distribution within the plant kingdom and lack of known function in primary metabolic pathways. Grapes fall into the list of high TA-accumulators, with biosynthesis occurring in both leaf and berry. Very little is known of the TA biosynthetic pathway enzymes in any plant species, although recently some progress has been made in this space. New technologies in grapevine research such as the development of global co-expression network analysis tools and genome-wide association studies, should enable more rapid progress. There is also a lack of information regarding roles for this organic acid in plant metabolism. Therefore this review aims to briefly summarize current knowledge about the key intermediates and enzymes of TA biosynthesis in grapes and the regulation of its precursor, ascorbate, followed by speculative discussion around the potential roles of TA based on current knowledge of Asc metabolism, TA biosynthetic enzymes and other aspects of fruit metabolism.

Exercise induces free radicals' overproduction and therefore, an enhancement of oxidative stress, defined as an imbalance between the production of reactive species and the intrinsic antioxidant defense. Redox activity of reactive species plays an important and a positive role on exercise adaptation, but these species at very high concentrations have detrimental effects. As a result, the use of antioxidant supplements for reducing oxidative stress can be an effective health strategy to maintain an optimal antioxidant status. In this sense, grapes are an important source of natural antioxidants due to their high content in polyphenols. They have shown antioxidant potential benefits for the reduction of intense exercise effect in athletes of different sport disciplines. Consequently, it is plausible to hypothesize that a strategic supplementation with grape based products may be a good approach to mitigate the exercise induced oxidative stress. The goal of this review is to present the state of the art of supplementation effects with grape beverages and grape extracts on the oxidative stress markers in athletes. The data of polyphenolic dosages, participant characteristics and exercise protocols are reported.

In the present work, a paste was developed from pomace and amaranth flour, which was obtained according to the methodology of Morato (2009), and later, it was mixed with amaranth in different concentrations to generate three formulations (50:50, 55:45,60:40) called M1, M2 and M3. M3 (Experimental) was the one that presented the highest levels of protein, fat, sugar, fiber and antioxidant content when compared to a commercial pasta (Yemina®) type spaghetti. The main results were a high content of protein and fiber (17.56 and 37.16 g/100 g), respectively. In the case of phenols, flavonoids and tannins, they were up to ten times higher compared to commercial pasta. The paste with pomace will be a new product, which if accepted sensory could give added value to the pomace and to the grape producers of the state of Queretaro., En el presente trabajo se desarrolló una pasta a partir de harina de orujo y amaranto, la cual se obtuvo de acuerdo con la metodología de Morato (2009), se mezcló con amaranto en diferentes concentraciones para generar tres formulaciones (50:50, 55:45, 60:40) denominadas M1, M2 y M3. La M3 (Experimental) fue la que presentó los niveles más altos en contenido de proteínas, grasas, azúcares, fibra y antioxidantes al compararla con una pasta comercial (Yemina®) tipo espagueti. Los principales resultadosfueron un alto contenido en proteína y fibra (17.56 y 37.16 g/100 g), respectivamente. En el caso de los fenoles, flavonoides y taninos fueron superiores hasta 10 veces comparados con la pasta comercial. La pasta con orujo será un producto nuevo, que de ser aceptado sensorialmente podría dar un valor agregado al orujo y a los productores de uva del estado de Querétaro.

In this study we have analyzed the anthocyanin composition of skin extracts of three red grape varieties Prokupac, Evita and Čokot Zemun in order to distinguish these cultivars based on their anthocyanin profile. Also, mechanical analysis of grape bunches and berries was performed. According to our results, seventeen anthocyanins were identified using LC‐MS technique and quantitative differences were recorded using HPLC‐DAD method. The highest content of total anthocyanins was obtained for Evita variety and the lowest one was recorded in Prokupac. Also, clear differences were observed in anthocyanins ratios. In comparison to Prokupac and Evita varieties, Čokot Zemun was characterized with a high content of cumaroyl derivatives of anthocyanin compounds, while high levels of acetylated derivatives were recorded in Prokupac. Data reported in this study represent a certain contribution to a database of mechanical properties and chemical composition of grape varieties originating from Balkan.

This study evaluates the capacity of four hydrolytic enzymes to limit the interactions between grape cell-walls and tannins and/or to favor tannin desorption. Adsorption and desorption tests were conducted by mixing a commercial seed tannin with purified skin cell-walls from Syrah grapes, in the presence or absence of hydrolytic enzymes, in a model-wine solution. The effects of the enzymes were evaluated by measuring the tannins in solution by High Performance Liquid Chromatography (HPLC) and the changes in the cell wall polysaccharide network by Comprehensive Microarray Polymer Profiling (COMPP) while the polysaccharides liberated from cell walls were analyzed by Size Exclusion Chromatography (SEC). The results showed that the enzymes limited the interaction between tannins and cell walls, especially cellulase, pectinase and xylanase, an effect associated with the cell wall structural modifications caused by the enzymes, which reduced their capacity to bind tannins. With regards to the tannin desorption process, enzymes did not play a significant role in liberating bound tannins. Those enzymes that showed the highest effect in limiting the adsorption of tannins and in disorganizing the cell wall structure, cellulase and pectinase, did not lead to a desorption of bound tannins, although they still showed a capacity of affecting cell wall structure. The results indicate that enzymes are not able to access those polysaccharides where tannins are bound, thus, they are not a useful tool for desorbing tannins from cell walls. The practical importance implications of these findings are discussed in the manuscript.