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As residências terapêuticas são dispositivos destinados a egressos de longas internações, sendo os cuidadores, mediadores desse processo. O objetivo desta pesquisa foi identificar a produção científica dos últimos 10 anos acerca da temática. Para isso, foi realizada uma busca nas bases de dados, Biblioteca Virtual em Saúde - Psicologia Brasil (BVS-Psi), Scielo, e CAPES Periódicos, com os descritores “cuidador” e “residência terapêutica”, totalizando 15 obras que foram analisadas por ano de publicação, categoria, tipo de estudo, instrumentos e materiais, resultados e lacunas. Os resultados indicaram maior prevalência de estudos em 2011 e menor em em 2010, 2015 e 2017, sendo a maioria de artigos estudos empíricos (81,25%) e com uso de entrevistas semi-estruturadas (45,45%). Em relação aos resultados observou-se que ser cuidador traz experiências de afetos ambíguos onde existem momentos de satisfação e insatisfação, que são permeados por sobrecarga física e emocional, falta de apoio assistencial, supervisão, capacitação e formações continuadas. Quanto às lacunas, nota-se a recomendação e a importância de que mais estudos sejam realizados com esses profissionais e que promovam espaços de escuta e cuidado à eles. Espera-se que esta pesquisa contribua para a importância de olhar os processos de saúde mental dos cuidadores, valorizando os papéis que são desempenhados por eles., Therapeutic Residential Service targets patients discharged after long-term hospitalization through the mediation of the care providers. The aim of this research was to identify the scientific production of the last ten years on the subject. A search on the Biblioteca Virtual em Saúde - Psicologia Brasil (BVS-Psi), Scielo, and CAPES Periódicos database was conducted using the following terms in Portuguese: “cuidador” and “residência terapêutica”. A total of 15 texts were analyzed considering its year of publication, category, type of study, instruments and materials, results, and gaps. The results show a higher prevalence of studies in 2011 a lower in 2010, 2015 and 2017. Most of the papers (81,25%) are empirical studies and used semi-structured interviews (45,45%). It was observed that being a care provider brings the experience of ambiguous feelings with moments of satisfaction and dissatisfaction. Physical and emotional overwhelming, and lack of support, supervision, training and continued education permeate the care providers experience. As for the gaps, it is noticeable the recommendation and justification of more studies on these professionals and the promotion of hearing and caring to their benefit. It is expected that this research contributes to stress the importance of considering the processes of mental health of the care providers, valuing the roles performed by them., Las residencias terapéuticas son dispositivos destinados a egresos de hospitalizaciones prolongadas, y los cuidadores son mediadores de ese proceso. El objetivo de esta investigación fue identificar la producción científica de los últimos diez años acerca de la temática. Para eso fue realizada una busca en las bases de dados Biblioteca Virtual em Saúde - Psicologia Brasil (BVS-Psi), Scielo, y CAPES Periódicos a través de los descriptores en portugués “cuidador” y “residencia terapêutica”. Un total de 15 textos fueron analizados considerando año de publicación, categoría, tipo de investigación, instrumentos y materiales, resultados y deficiencias identificadas. Los resultados indicaron una mayor prevalencia de investigaciones en 2011 y menor en 2010, 2015 y 2017. La mayoría de los textos (81,25%) son estudios empíricos y utilizan entrevistas semiestructuradas (45,45%). Con relación a los resultados, se observó que ser cuidador provoca la experiencia de afectos ambiguos, con momentos de satisfacción y insatisfacción. Sobrecarga emocional y física y falta de apoyo, supervisión, capacitación y formación continua permean la experiencia de los cuidadores. Cuanto a las deficiencias identificadas, notase la recomendación y reconocimiento de la importancia de realizarse más investigaciones con estes profesionales y que sean promovidos espacios de escucha y cuidado en su beneficio. Esperase que esta investigación contribuya para estresar la importancia de se considerar los procesos de salud mental de los cuidadores, valorando los papeles desempeñados por ellos.

As residências terapêuticas são dispositivos destinados a egressos de longas internações, sendo os cuidadores, mediadores desse processo. O objetivo desta pesquisa foi identificar a produção científica dos últimos 10 anos acerca da temática. Para isso, foi realizada uma busca nas bases de dados, Biblioteca Virtual em Saúde - Psicologia Brasil (BVS-Psi), Scielo, e CAPES Periódicos, com os descritores “cuidador” e “residência terapêutica”, totalizando 15 obras que foram analisadas por ano de publicação, categoria, tipo de estudo, instrumentos e materiais, resultados e lacunas. Os resultados indicaram maior prevalência de estudos em 2011 e menor em em 2010, 2015 e 2017, sendo a maioria de artigos estudos empíricos (81,25%) e com uso de entrevistas semi-estruturadas (45,45%). Em relação aos resultados observou-se que ser cuidador traz experiências de afetos ambíguos onde existem momentos de satisfação e insatisfação, que são permeados por sobrecarga física e emocional, falta de apoio assistencial, supervisão, capacitação e formações continuadas. Quanto às lacunas, nota-se a recomendação e a importância de que mais estudos sejam realizados com esses profissionais e que promovam espaços de escuta e cuidado à eles. Espera-se que esta pesquisa contribua para a importância de olhar os processos de saúde mental dos cuidadores, valorizando os papéis que são desempenhados por eles., Therapeutic Residential Service targets patients discharged after long-term hospitalization through the mediation of the care providers. The aim of this research was to identify the scientific production of the last ten years on the subject. A search on the Biblioteca Virtual em Saúde - Psicologia Brasil (BVS-Psi), Scielo, and CAPES Periódicos database was conducted using the following terms in Portuguese: “cuidador” and “residência terapêutica”. A total of 15 texts were analyzed considering its year of publication, category, type of study, instruments and materials, results, and gaps. The results show a higher prevalence of studies in 2011 a lower in 2010, 2015 and 2017. Most of the papers (81,25%) are empirical studies and used semi-structured interviews (45,45%). It was observed that being a care provider brings the experience of ambiguous feelings with moments of satisfaction and dissatisfaction. Physical and emotional overwhelming, and lack of support, supervision, training and continued education permeate the care providers experience. As for the gaps, it is noticeable the recommendation and justification of more studies on these professionals and the promotion of hearing and caring to their benefit. It is expected that this research contributes to stress the importance of considering the processes of mental health of the care providers, valuing the roles performed by them., Las residencias terapéuticas son dispositivos destinados a egresos de hospitalizaciones prolongadas, y los cuidadores son mediadores de ese proceso. El objetivo de esta investigación fue identificar la producción científica de los últimos diez años acerca de la temática. Para eso fue realizada una busca en las bases de dados Biblioteca Virtual em Saúde - Psicologia Brasil (BVS-Psi), Scielo, y CAPES Periódicos a través de los descriptores en portugués “cuidador” y “residencia terapêutica”. Un total de 15 textos fueron analizados considerando año de publicación, categoría, tipo de investigación, instrumentos y materiales, resultados y deficiencias identificadas. Los resultados indicaron una mayor prevalencia de investigaciones en 2011 y menor en 2010, 2015 y 2017. La mayoría de los textos (81,25%) son estudios empíricos y utilizan entrevistas semiestructuradas (45,45%). Con relación a los resultados, se observó que ser cuidador provoca la experiencia de afectos ambiguos, con momentos de satisfacción y insatisfacción. Sobrecarga emocional y física y falta de apoyo, supervisión, capacitación y formación continua permean la experiencia de los cuidadores. Cuanto a las deficiencias identificadas, notase la recomendación y reconocimiento de la importancia de realizarse más investigaciones con estes profesionales y que sean promovidos espacios de escucha y cuidado en su beneficio. Esperase que esta investigación contribuya para estresar la importancia de se considerar los procesos de salud mental de los cuidadores, valorando los papeles desempeñados por ellos.

Despite their therapeutic potential, many protein drugs remain inaccessible to patients since they are difficult to secrete. Each recombinant protein has unique physicochemical properties and requires different machinery for proper folding, assembly, and posttranslational modifications (PTMs). Here we aimed to identify the machinery supporting recombinant protein secretion by measuring the protein-protein interaction (PPI) networks of four different recombinant proteins (SERPINA1, SERPINC1, SERPING1, and SeAP) with various PTMs and structural motifs using the proximity-dependent biotin identification (BioID) method. We identified PPIs associated with specific features of the secreted proteins using a Bayesian statistical model and found proteins involved in protein folding, disulfide bond formation, and N-glycosylation were positively correlated with the corresponding features of the four model proteins. Among others, oxidative folding enzymes showed the strongest association with disulfide bond formation, supporting their critical roles in proper folding and maintaining the ER stability. Knockdown of disulfide-isomerase PDIA4, a measured interactor with significance for SERPINC1 but not SERPINA1, led to the decreased secretion of SERPINC1, which relies on its extensive disulfide bonds, compared to SERPINA1, which has no disulfide bonds. Proximity-dependent labeling successfully identified the transient interactions supporting synthesis of secreted recombinant proteins and refined our understanding of key molecular mechanisms of the secretory pathway during recombinant protein production.

Despite their therapeutic potential, many protein drugs remain inaccessible to patients since they are difficult to secrete. Each recombinant protein has unique physicochemical properties and requires different machinery for proper folding, assembly, and post-translational modifications (PTMs). Here we aimed to identify the machinery supporting recombinant protein secretion by measuring the protein-protein interaction (PPI) networks of four different recombinant proteins (SERPINA1, SERPINC1, SERPING1 and SeAP) with various PTMs and structural motifs using the proximity-dependent biotin identification (BioID) method. We identified PPIs associated with specific features of the secreted proteins using a Bayesian statistical model, and found proteins involved in protein folding, disulfide bond formation and N-glycosylation were positively correlated with the corresponding features of the four model proteins. Among others, oxidative folding enzymes showed the strongest association with disulfide bond formation, supporting their critical roles in proper folding and maintaining the ER stability. Knockdown of disulfide-isomerase PDIA4, a measured interactor with significance for SERPINC1 but not SERPINA1, led to the decreased secretion of SERPINC1, which relies on its extensive disulfide bonds, compared to SERPINA1, which has no disulfide bonds. Proximity-dependent labeling successfully identified the transient interactions supporting synthesis of secreted recombinant proteins and refined our understanding of key molecular mechanisms of the secretory pathway during recombinant protein production. This article is protected by copyright. All rights reserved.

Sepsis is an extreme host response to infection that leads to loss of organ function and cardiovascular integrity. Mortality from sepsis is on the rise. Despite more than three decades of research and clinical trials, specific diagnostic and therapeutic strategies for sepsis are still absent. The use of LFQ- and TMT-based quantitative proteomics is reported here to study the plasma proteome in five mouse models of sepsis. A knowledge-based interpretation of the data reveals a protein network with extensive connectivity through documented functional or physical interactions. The individual proteins in the network all have a documented role in sepsis and are known to be extracellular. The changes in protein abundance observed in the mouse models of sepsis have for the most part the same directionality (increased or decreased abundance) as reported in the literature for human sepsis. This network has been named the Plasma Proteome Signature of Sepsis (PPSS). The PPSS is a quantifiable molecular readout that can supplant the current symptom-based approach used to diagnose sepsis. This type of molecular interpretation of sepsis, its progression, and its response to therapeutic intervention are an important step in advancing our understanding of sepsis, and for discovering and evaluating new therapeutic strategies.

Sepsis is an extreme host response to infection that leads to loss of organ function and cardiovascular integrity. Mortality from sepsis is on the rise. Despite more than three decades of research and clinical trials, specific diagnostic and therapeutic strategies for sepsis are still absent. The use of LFQ- and TMT-based quantitative proteomics is reported here to study the plasma proteome in five mouse models of sepsis. A knowledge-based interpretation of the data reveals a protein network with extensive connectivity through documented functional or physical interactions. The individual proteins in the network all have a documented role in sepsis and are known to be extracellular. The changes in protein abundance observed in the mouse models of sepsis have for the most part the same directionality (increased or decreased abundance) as reported in the literature for human sepsis. This network has been named the Plasma Proteome Signature of Sepsis (PPSS). The PPSS is a quantifiable molecular readout that can supplant the current symptom-based approach used to diagnose sepsis. This type of molecular interpretation of sepsis, its progression, and its response to therapeutic intervention are an important step in advancing our understanding of sepsis, and for discovering and evaluating new therapeutic strategies.

Sepsis is an extreme host response to infection that leads to loss of organ function and cardiovascular integrity. Mortality from sepsis is on the rise. Despite more than three decades of research and clinical trials, specific diagnostic and therapeutic strategies for sepsis are still absent. The use of LFQ- and TMT-based quantitative proteomics is reported here to study the plasma proteome in five mouse models of sepsis. A knowledge-based interpretation of the data reveals a protein network with extensive connectivity through documented functional or physical interactions. The individual proteins in the network all have a documented role in sepsis and are known to be extracellular. The changes in protein abundance observed in the mouse models of sepsis have for the most part the same directionality (increased or decreased abundance) as reported in the literature for human sepsis. This network has been named the Plasma Proteome Signature of Sepsis (PPSS). The PPSS is a quantifiable molecular readout that can supplant the current symptom-based approach used to diagnose sepsis. This type of molecular interpretation of sepsis, its progression, and its response to therapeutic intervention are an important step in advancing our understanding of sepsis, and for discovering and evaluating new therapeutic strategies.

La botriomicosis es una mortal e infrecuente infección bacteriana granulomatosa supurativa. Presentamos el caso de una paciente de 52 años que reingresó por cuadro respiratorio complicado dos meses anteriores, con síntomas caracterizados por tos, disnea, alza térmica, inapetencia y pérdida de peso. En la tomografía pulmonar se observó fibrosis, infiltrado alveolar bilateral, cavidades y bronquiectasias bilaterales. La citología de aspirado bronquial mostró gránulos azurófilos haciendo sospechar de actinomicosis, por lo que se le dio tratamiento con penicilina, sin mejoría. En el segundo cultivo de aspirado bronquial el patólogo describió a dos grupos de colonias bacterianas gramnegativas de Escherichia coli, las cuales simularon gránulos azurófilos, planteándose como diagnóstico definitivo botriomicosis pulmonar por E. coli BLEE, no obstante, la paciente ya había fallecido. Como conclusión, se reporta una patología infecciosa de muy baja prevalencia, pero frecuentemente confundida con la Actinomicosis, cuyo diagnóstico temprano es extremadamente importante para reducir su alta mortalidad.

Objective: To identify the number of incident cases of Influenza A H1N1 Pandemic during and after 2009-2010. The study: An observational, descriptive and transversal study. Done in the Hipolito Unanue National Hospital (HNHU). 809 patients suspected Influenza A H1N1atendidos Hospital were included in 2009 (May to December) and 2010 (January to September). Findings: 85 were hospitalized and 11 died in 2009 In 2010, 89 suspected cases were reported, and 7 were positive. Case fatality rate was 0.21 per 100 cases and the most common age group over 60 years (15.7%). Conclusions: 52 new cases of Influenza A H1N1diagnosed in HNHU during May 2009 - September 2010., Objetivo: Identificar el número de casos incidentes de Influenza A H1N1 durante y post Pandemia 2009-2010. El Estudio: Estudio observacional, descriptivo y transversal. Realizado en el Hospital Nacional Hipólito Unanue (HNHU). Se incluyeron a 809 pacientes sospechosos de Influenza A H1N1atendidos en el Hospital durante el 2009 (Mayo a Diciembre) y el 2010 (Enero a Setiembre). Hallazgos: 85 fueron hospitalizados y 11 fallecieron en el 2009. En el 2010 se notificó 89 casos sospechosos, y 7 dieron positivos. La tasa de Letalidad fue 0,21 por 100 casos y el grupo etáreo más frecuente los mayores de 60 años (15,7%). Conclusiones: 52 casos incidentes de Influenza A H1N1diagnósticados en el HNHU durante Mayo 2009- Setiembre 2010.

Objetive: To identify the incidence of features of eating disorders and of consumption of psychoactive substances in Human Medicine students of a private university of Lima, 2012. Materials and Methods: It is of kind observational, descriptive and transversal, its level is descriptive and its design is epidemiological. The study population consisted of students from first to fifth cycle of Human Medicine a private university in Lima, from which was extracted Probabilistically and Randomly a sample of 109 students. The technique was survey and the data was analyzed using the program Microsoft Excel 2010 and SPSS 19. Results: From the 109 (100%) students of Human Medicine, the most frequent features of eating disorders were interoceptive awareness and ineffectiveness and low self-esteem, both with 53 (48.6%) students of Human Medicine. Likewise psychoactive substances were firstly consumed alcohol 90 (82.5%) students, followed by the snuff with 26 (23.9%) of them. Conclusions: We identify that in the students of Human Medicine of the current study the incidence for features of eating disorders was of 48.6% and for the consumption of psychoactive substance was of 82.5%., Objetivo: Identificar la incidencia de rasgos de trastornos de conducta alimentaria y de consumo de sustancias psicoactivas en estudiantes de Medicina Humana de una Universidad Privada de Lima; 2012. Materiales y Métodos: Estudio de tipo observacional, descriptivo y transversal; de nivel descriptivo y de diseño epidemiológico. La población de estudio fueron estudiantes de primero a quinto ciclo de Medicina Humana de una Universidad Privada de Lima; de la cual se extrajo de manera Probabilística-Aleatoria Simple una muestra de 109 estudiantes. La técnica fue encuesta y se analizaron los datos mediante los programas Microsoft Excel 2010 y SPSS 19. Resultados: De los 109 (100%) estudiantes de Medicina Humana, los rasgos de trastornos de conducta alimentaria que obtuvieron una mayor frecuencia fueron de Conciencia Interoceptiva y de Inefectividad y Baja Autoestima, ambos con 53 (48,6%) estudiantes de Medicina Humana. Así mismo las sustancias psicoactivas más consumidas fueron en primer lugar el alcohol con 90 (82,5%) estudiantes, seguido por el tabaco con 26 (23,9%) de los mismos. Conclusiones: Se identificó que en los estudiantes de Medicina Humana del presente estudio, la incidencia para rasgos de trastornos de conducta alimentaria fue de 48,6% y para el consumo de sustancias psicoactivas fue de 82,5%.