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415 results on '"Campus France"'

1. Mating Interactions between Schistosoma bovis and S. mansoni and Compatibility of Their F1 Progeny with Biomphalaria glabrata and Bulinus truncatus

Author: Amos Mathias Onyekwere, Alejandra De Elias-Escribano, Julien Kincaid-Smith, Sarah Dametto, Jean-François Allienne, Anne Rognon, Maria Dolores Bargues, Jérôme Boissier, Alex Ekwueme Federal University Ndufu-Alike, Ikwo (AE-FUNAI), Interactions Hôtes-Pathogènes-Environnements (IHPE), Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Departamento de Parasitologia, Universitat de València (UV)-Facultad de farmacia, Facultad de Farmacia, Universitat de València (UV), CIBER de Enfermedades Infecciosas, Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), This research was funded by Campus France, France and Tertiary Education Trust Fund (TETfund), Nigeria and was supported by the program HySWARM (ANR-18-CE35-0001) from the French Research National Agency respectively awarded to J.B. and A.M.O. is funded by Campus France. M.D.B. and A.D.E.-E. were funded by the Red de Investigacion de Centros de Enfermedades Tropicales-RICET (RD16/0027/0023) of the PN de I+D+I, ISCIII-Subdireccion General de Redes y Centros de Investigacion Cooperativa RETICS, Ministry of Health and Consumption, Madrid, by CIBER de Enfermedades Infecciosas (CB21/13/00056), ISCIII, Ministry of Science and Education, Madrid, Spain, by the PROMETEO Program, Programa de Ayudas para Grupos de Investigacion de Excelencia (2021/004), Generalitat Valenciana, Valencia, Spain and by BEFPI/2021/019. This study is set within the framework of the 'Laboratoires d'Excellence (LABEX)' TULIP (ANR-10-LABX-0041)., ANR-18-CE35-0001,HySWARM,Hybrid Swarm: rôle de l'hybridation dans les capacités invasives, l'épidémiologie et le diagnostic de la schistosomiase(2018), and ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010)
Subjects: Microbiology (medical), mating interactions, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], fungi, Schistosoma mansoni, compatibility, Microbiology, Biomphalaria glabrata, Schistosoma bovis, F1 progeny, Bulinus truncatus, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction, Virology, parasitic diseases, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Abstract: Contrary to the majority of other Trematoda, Schistosoma species are gonochoric. Consequently, in endemic areas where several schistosome species overlap and can co-infect the same definitive host, there may be frequent opportunities for interspecific pairing. Our experimental study provides novel insight on the pairing behavior between Schistosoma bovis and S. mansoni in mixed infections in mice. We used six mate choice experiments to assess mating interactions between the two schistosome species. We show that mating between the two Schistosoma species is not random and that S. mansoni exhibits greater mate recognition compared to S. bovis. We also performed reciprocal crosses (male S. mansoni × female S. bovis) and (female S. mansoni × male S. bovis) that produce active swimming miracidia. These miracidia were genotyped by ITS2 sequencing and proposed for mollusc infection. Molecular analyses show that all the miracidia are parthenogenetically produced (i.e., their harbor the mother ITS2 genotype) and as a consequence can only infect the mollusc of the maternal species. Offspring produced by male S. mansoni × female S. bovis pairing can only infect Bulinus truncatus whereas offspring produced by female S. mansoni × male S. bovis can only infect Biomphalaria glabrata snails. Evolutionary and epidemiological consequences are discussed.
Published: 2022

2. Rejection of small charged and uncharged organic pollutants in reverse osmosis of salty and seawaters

Author: Omar Samhari, Fatima Zohra Charik, Aissa Belouatek, Saad Alami Younssi, Murielle Rabiller-Baudry, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), University Hassan II [Casablanca], Université Abdelhamid Ibn Badis de Mostaganem, CEDOC program (France-Morocco cooperation) Campus France [2017/08 100], Eiffel Campus France [P757215H], and Univ. Mostaganem
Subjects: Micropollutant, Ethanol, Desalination, Reverse osmosis, [CHIM]Chemical Sciences, Seawater, Thiamine, Tropaeolin O, Vitamin, Transfer mechanism, Azo dye
Abstract: International audience; Two organic solutes of close molecular weight (approximate to 270 g mol(-1)) and hydrophobicity (logK(ow) approximate to 1.2- 1.5) were selected as model micropollutants to study their reverse osmosis in seawater. Tropaeolin O can be a monovalent/divalent anion whereas Thiamine can be a monovalent/divalent cation. Their charges and hydrophilic/hydrophobic balance can be modulated by playing on the pH and the ionic strength. The TFC-HR (Koch, USA) and SW30 (FilmTec, The Netherlands) membranes have shown a similar overall behavior either for single NaCl or Tropaeolin O filtrations. The comparison of Tropaeolin O/Thiamine rejections by the TFC-HR membrane has shown that membrane/solute electrostatic interactions explained the rejection in single solutions. However, in 30 g L-1 NaCl and synthetic seawater, rejections were more likely due to the hydrophobic/hydrophilic balance of a given solute: the more hydrophobic form being more rejected than the hydrophilic ones. Finally, the ethanol addition (30%) was achieved to study the impact of a second concentrated organics, moreover able to modify the background solvent of the filtration; Tropaeolin O rejection was decreased.
Published: 2022

3. Design and Modeling of a Flexible Conductive Fabric Antenna Integrated in an OLED Light Source for WIMAX Wireless Communication Systems

Author: Mustapha El Halaoui, Laurent Canale, Soukaina Sekkal, Othmane Bendaou, Adel Asselman, Optics and Photonics Laboratory, Université Abdelmalek Essaadi, Tetouan, Morocco, Faculté des Sciences de Tétouan, LAboratoire PLasma et Conversion d'Energie (LAPLACE), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Lumière et Matière (LAPLACE-LM), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Centre National de la Recherche Scientifique (CNRS), Modeling and Simulation of Mechanical Systems Laboratory, Université Abdelmalek Essaâdi (UAE), This work is supported by a grant from the PHC Maghreb (Hubert Curien Program) MELINA (Mastering Efficient Lighting in North Africa). Funding is provided by Campus France (Grant number 43981ZG awarded to L.C.) and the French Ministries of Europe and Foreign Affairs, and in Morocco, by the Ministry of Higher Education and Scientific Research, in partnership with the National Center for Scientific and Technical Research (CNRST)., and PHC Maghreb (Hubert Curien Program) MELINA (Mastering Efficient Lighting in North Africa). Campus France (Grant number 43981ZG)
Subjects: Materials science, Wireless Communication Systems, 02 engineering and technology, Flexible Antenna, 7. Clean energy, Radiation pattern, 0202 electrical engineering, electronic engineering, information engineering, OLED, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Reflection coefficient, Electrical conductor, Organic Light-Emitting Diode, business.industry, [SPI.NRJ]Engineering Sciences [physics]/Electric power, 020206 networking & telecommunications, 021001 nanoscience & nanotechnology, WiMAX, [SPI.TRON]Engineering Sciences [physics]/Electronics, Transparent Conductive Fabric, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Optoelectronics, Antenna (radio), 0210 nano-technology, business, Layer (electronics), Microwave
Abstract: International audience; This work presents a new bendable antenna for worldwide interoperability for microwave access (WiMAX) wireless communication systems. These antennas, transparent and flexible, will be easily integrated into various mdia and in particular OLED lighting which could be part of the public lighting network of tomorrow as well as on all display media. The integration of these antennas as close as possible to the end-user is a possible solution to reduce the energy consumption which goes hand in hand with the increase in the data rate. This kind of new antenna, designed to be integrated in organic light-emitting diode (OLED), was modeled from a transparent VeilShieldTM conductive fabric and was placed on a 100% polyester substrate with a thickness of 1.5 mm and a loss tangent of 0.02. We have tested and evaluated the characteristic parameters of our antenna, namely the reflection coefficient, the radiation pattern and the gain, to find out the performance of our proposed design. The performance of the transparent conductive fabric integrated in the 100% polyester substrate is tested for the application of flexible antenna operating at 3.5 GHz with a gain value of 5.38 dB. We have integrated this proposed new antenna with the OLED light source containing four layers of different materials and electrical properties: aluminum cathode layer, polymer layer, indium tin oxide (ITO) anode layer and glass substrate layer. After integration, the resonant frequency shifted to 3.52 GHz with a gain value of 4.61 dB. In addition, we also tested the concave bending on the reflection coefficient of the proposed flexible antenna taking into account the different bending angles. This work demonstrates the possibility of integrating these unconventional materials used for the proposed antenna within the OLED despite weak effects on the resonant frequency and the gain of the proposed antenna after integration.
Published: 2021

4. Occupational exposure to disinfectants and asthma incidence in U.S. nurses: A prospective cohort study

Author: Carlos A. Camargo, Frank E. Speizer, Paul K. Henneberger, Raphaëlle Varraso, Nicole Le Moual, Catherine Quinot, Orianne Dumas, Jan-Paul Zock, Krislyn M. Boggs, Vieillissement et Maladies chroniques : approches épidémiologique et de santé publique (VIMA), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), PCOFUND-GA-2013-609102 National Institutes of Health, NIH Centers for Disease Control and Prevention, CDC: UM1 CA‐176726 Marie Curie Research Executive Agency, REA UniversitÃ© Pierre et Marie Curie, UPMC, The Nurses? Health Study II is coordinated at the Channing Division of Network Medicine, Brigham and Women's Hospital, Boston, MA. We would like to thank the participants and staff of the Nurses? Health Study II for their valuable contributions. Support for this study was provided by grants R01 OH-010359 (Centers for Disease Control and Prevention), UM1 CA-176726 (National Institutes of Health) and funding from the People Program (Marie Curie Actions) of the European Union's Seventh Framework Program (FP7/2007-2013) under REA grant agreement no. PCOFUND-GA-2013-609102, through the PRESTIGE program coordinated by Campus France. John Meyer declares that he has no conflict of interest in the review and publication decision regarding this article., and The Nurses’ Health Study II is coordinated at the Channing Division of Network Medicine, Brigham and Women's Hospital, Boston, MA. We would like to thank the participants and staff of the Nurses’ Health Study II for their valuable contributions. Support for this study was provided by grants R01 OH‐010359 (Centers for Disease Control and Prevention), UM1 CA‐176726 (National Institutes of Health) and funding from the People Program (Marie Curie Actions) of the European Union's Seventh Framework Program (FP7/2007‐2013) under REA grant agreement no. PCOFUND‐GA‐2013‐609102, through the PRESTIGE program coordinated by Campus France.
Subjects: Adult, Disinfectant, Population, Nurses, Article, 03 medical and health sciences, 0302 clinical medicine, Occupational Exposure, Surveys and Questionnaires, Environmental health, medicine, Humans, Prospective Studies, 030212 general & internal medicine, Asthma, Occupational, Prospective cohort study, education, disinfection, Asthma, 2. Zero hunger, education.field_of_study, healthcare workers, business.industry, Incidence, Hazard ratio, Public Health, Environmental and Occupational Health, Middle Aged, asthma, medicine.disease, United States, Confidence interval, 3. Good health, 030228 respiratory system, Female, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, cleaning products, occupational asthma, business, Occupational asthma, Body mass index, Disinfectants
Abstract: International audience; Background: Exposure to disinfectants among healthcare workers has been associated with respiratory health effects, in particular, asthma. However, most studies are cross-sectional and the role of disinfectant exposures in asthma development requires longitudinal studies. We investigated the association between occupational exposure to disinfectants and incident asthma in a large cohort of U.S. female nurses. Methods: The Nurses’ Health Study II is a prospective cohort of 116 429 female nurses enrolled in 1989. Analyses included 61 539 participants who were still in a nursing job and with no history of asthma in 2009 (baseline; mean age: 55 years). During 277 744 person-years of follow-up (2009-2015), 370 nurses reported incident physician-diagnosed asthma. Occupational exposure was evaluated by questionnaire and a Job-Task-Exposure Matrix (JTEM). We examined the association between disinfectant exposure and subsequent asthma development, adjusted for age, race, ethnicity, smoking status, and body mass index. Results: Weekly use of disinfectants to clean surfaces only (23% exposed) or to clean medical instruments (19% exposed) was not associated with incident asthma (adjusted hazard ratio [95% confidence interval] for surfaces, 1.12 [0.87-1.43]; for instruments, 1.13 [0.87-1.48]). No association was observed between high-level exposure to specific disinfectants/cleaning products evaluated by the JTEM (formaldehyde, glutaraldehyde, bleach, hydrogen peroxide, alcohol quats, or enzymatic cleaners) and asthma incidence. Conclusions: In a population of late career nurses, we observed no significant association between exposure to disinfectants and asthma incidence. A potential role of disinfectant exposures in asthma development warrants further study among healthcare workers at earlier career stage to limit the healthy worker effect.
Published: 2019

5. The Levantine Intermediate Water in the western Mediterranean and its interactions with the Algerian Gyres: insights from 60 years of observation

Author: Katia Mallil, Pierre Testor, Anthony Bosse, Félix Margirier, Loic Houpert, Hervé Le Goff, Laurent Mortier, Ferial Louanchi, Ecole Nationale Supérieure des Sciences de la Mer et de l'Aménagement du Littoral (ESSMAL), Variabilité de l'Océan et de la Glace de mer (VOG), Laboratoire d'Océanographie et du Climat : Expérimentations et Approches Numériques (LOCEAN), Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), School of Earth and Atmospheric Sciences [Atlanta], Georgia Institute of Technology [Atlanta], OSE Engineering, Développement Instrumental et Techniques Marines (DITM), and Campus France (PHC Tassili (grant no. 35163NA) and the Campus France (PROFAS B+ (grant no. 907058C).
Subjects: 13. Climate action, 14. Life underwater, General Medicine, [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography
Abstract: The presence of two large scale cyclonic gyres in the Algerian basin influences the general and eddy circulation, but their effect on water mass transfer remain poorly characterized. Our study has confirmed the presence of these gyres using the first direct current measurements of the whole water column collected during the SOMBA-GE2014 cruise, specifically designed to investigate these gyres. Using cruise sections and a climatology from 60 years of in situ measurements, we have also shown the effect of these gyres on the distribution at intermediate depth of Levantine Intermediate Water (LIW) with warmer (~0.15 °C) and saltier (~0.02 g.kg−1) characteristics in the Algerian basin than in the Provençal basin. The Algerian gyres also impact horizontal density gradients with sinking of the isopycnals at the gyres’ centres. Temporal cross-correlation of LIW potential temperature referenced to the signal observed south of Sardinia reveal timescale of transit of 4 months to get to the centre of the Algerian basin. The LIW temperature and salinity trends over various periods are estimated to: +0.0017 ± 0.0014 °C.year−1 and +0.0017 ± 0.0003 year−1 respectively over the 1960–2017 period, and accelerating to +0.059 ± 0.072 °C.year−1 and +0.013 ± 0.006 year−1 over the 2013–2017 period.
Published: 2021

6. Impact of sodium hypochlorite on rejection of non-steroidal anti-inflammatory drugs by biomimetic forward osmosis membranes

Author: Kamel Daoud, Maxime Pontié, Irena Petrinić, Patrick Loulergue, Claus Hélix-Nielsen, Noureddine Nasrallah, Anthony Szymczyk, Walid Ghamri, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université des Sciences et de la Technologie Houari Boumediene = University of Sciences and Technology Houari Boumediene [Alger] (USTHB), University of Maribor, Danmarks Tekniske Universitet = Technical University of Denmark (DTU), Université d'Angers (UA), The Ministry of Higher Education and Scientific Research of Algeria and Campus France are gratefully acknowledged for providing W. Ghamri with a Profas B+ grant (960923G). Campus France is also acknowledged for financial support through PHC Proteus program (40023WM). I. Petrinic and C. Hélix-Nielsen gratefully acknowledge support from the NepWat project supported by the NovoNordisk Foundation (grant NNF18OC0034918)., Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université des Sciences et de la Technologie Houari Boumediene [Alger] (USTHB), and Technical University of Denmark [Lyngby] (DTU)
Subjects: Biomimetic membranes, Forward osmosis, chemistry.chemical_element, Filtration and Separation, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biochemistry, chemistry.chemical_compound, Chlorine, [CHIM]Chemical Sciences, General Materials Science, Physical and Theoretical Chemistry, Concentration polarization, Chemistry, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Pharmaceutical compounds, Sodium hypochlorite, Ageing, Membrane, Chemical engineering, Polyamide, Polymersome, 0210 nano-technology, Flux (metabolism)
Abstract: International audience; Biomimetic forward osmosis membranes with a polyamide-based active layer containing polymersomes incorporating aquaporins were exposed to various free chlorine doses (free chlorine concentration x exposure time) and pH. Membrane chlorination was found to decrease the apolar/polar balance of the membrane surface energy and to increase its negative surface charge density. A substantial modification of the hydrogen-bond network in the membrane active layer was also highlighted. The separation performance of biomimetic membranes was also impacted by chlorine-induced chemical ageing. For the lowest free chlorine doses (100 ppm h), the reverse salt flux was found to increase with respect to the pristine membrane while the water flux dropped by 10-20%. For higher free chlorine doses (1000 and 10 000 ppm h), both the reverse salt flux and the water flux were found higher than for the pristine membrane. These behaviors were explained by the combination of several mechanisms with possible antagonist effects, namely a reduced internal concentration polarization due to ageing of the membrane support material, and modifications of the hydrogen-bond network and surface charge density of the membrane active layer. Although the degradation caused by sodium hypochlorite increased the draw-solute loss per unit of water passed, the biomimetic membranes maintained their rejection ability towards naproxen and diclofenac as rejections higher than 95.9% were measured irrespective of the ageing conditions.
Published: 2021

7. The neuroprotective activity of heat-treated human platelet lysate biomaterials manufactured from outdated pathogen-reduced (amotosalen/UVA) platelet concentrates

Author: Lassina Barro, Valérie Vingtdeux, Ouada Nebie, David Blum, Jean Christophe Devedjian, Ming Li Chou, Folke Knutson, Thierry Burnouf, Régis Bordet, Luc Buée, Aurélie Jonneaux, David Devos, Graduate Institute of Biomedical Materials and Tissue Engineering [Taipei, Taiwan] (College of Biomedical Engineering), College of Biomedical Engineering [Taipei, Taiwan]-Taipei Medical University, Troubles cognitifs dégénératifs et vasculaires - U 1171 (TCDV), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U1172 Inserm - U837 (JPArc), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE)-Université de Lille, International Ph.D. Program in Biomedical Engineering [Taipei, Taiwan] (College of Biomedical Engineering), Taipei Medical University-College of Biomedical Engineering [Taipei, Taiwan], Clinical Immunology and Transfusion Medicine IGP [Uppsala, Sweden], Uppsala University, The study was supported in part by grant 107–2314-B-038-084 from the Ministry of Science and Technology (MOST) of Taiwan and Taipei Medical University (TMU) Higher Education Sprout Project MoE: DP2–107-21121-01 N-09 to TB’s laboratory. ON was supported by a PhD fellowship from TMU anda CABRI grant from the Université de Lille, France. The cooperation betweenTB’s laboratory of TMU and Inserm UMR-S1172 is supported by a bilateralOrchid research project (MOST and French Association of Taiwan-Campus France) N° 108–2911-I-038-503. LB and DB’s laboratory is supported by Programme d’investissements d’avenir LabEx (excellence laboratory) DISTALZ (Development of Innovative Strategies for a Transdisciplinary approach to ALZheimer’s disease), ANR, Fondation pour la Recherche Médicale, Vaincre Alzheimer, Fondation Plan Alzheimer as well as Inserm, CNRS, Université de Lille, Lille Métropole Communauté Urbaine, Région Hauts-de-France, DN2M., Blum, David, Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 (JPArc), Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), International Ph.D. Program in Cell Therapy and Regeneration Medicine [Taipei, Taiwan], Taipei Medical University, The study was supported in part by grant 107–2314-B-038-084 from the Ministry of Science and Technology (MOST) of Taiwan and Taipei Medical University (TMU) Higher Education Sprout Project MoE: DP2–107-21121-01 N09 to TB’s laboratory. ON was supported by a PhD fellowship from TMU and a CABRI grant from the Université de Lille, France. The cooperation between TB’s laboratory of TMU and Inserm UMR-S1172 is supported by a bilateral Orchid research project (MOST and French Association of Taiwan-Campus France) N° 108–2911-I-038-503. LB and DB’s laboratory is supported by Programme d’investissements d’avenir LabEx (excellence laboratory) DISTALZ (Development of Innovative Strategies for a Transdisciplinary approach to ALZheimer’s disease), ANR, Fondation pour la Recherche Médicale, Vaincre Alzheimer, Fondation Plan Alzheimer as well as Inserm, CNRS, Université de Lille, Lille Métropole Communauté Urbaine, Région Hauts-de-France, DN2M., CHU Lille, CNRS, Inserm, Université de Lille, Troubles cognitifs dégénératifs et vasculaires - U1171, Lille Neurosciences & Cognition (LilNCog) - U 1172, Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer (JPArc) - U1172, Troubles cognitifs dégénératifs et vasculaires - U 1171 - EA 1046 (TCDV), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche Saint-Antoine (CR Saint-Antoine), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and BUEE, Luc
Subjects: 0301 basic medicine, Hot Temperature, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, lcsh:Medicine, Biocompatible Materials, 030204 cardiovascular system & hematology, Pharmacology, Mice, 0302 clinical medicine, Neurotrophic factors, Furocoumarins, Pharmacology (medical), Platelet, Neurons, medicine.diagnostic_test, Chemistry, Parkinson Disease, General Medicine, Neuroprotection, 3. Good health, Neuroprotective Agents, Pathogen inactivation, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Blood Platelets, Amotosalen, Programmed cell death, Ultraviolet Rays, Cellbiologi, Cell Line, 03 medical and health sciences, Western blot, Primary neurons, medicine, Animals, Humans, Ferroptosis, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Viability assay, Synaptic markers, Molecular Biology, Intercept-platelet lysate, Research, Biochemistry (medical), LUHMES cells, lcsh:R, Cell Biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, 030104 developmental biology, Cell culture
Abstract: Background Effective neurorestorative therapies of neurodegenerative diseases must be developed. There is increasing interest in using human platelet lysates, rich in neurotrophic factors, as novel disease-modifying strategy of neurodegeneration. To ensure virus safety, pathogen reduction treatments should be incorporated in the preparation process of the platelet concentrates used as source material. We therefore investigated whether platelet concentrates (PC) pathogen-inactivated using a licensed photo-inactivation treatment combining photosensitive psoralen (amotosalen) and UVA irradiation (Intercept) can serve as source material to prepare platelet lysates with preserved neuroprotective activity in Parkinson’s disease models. Methods Intercept treated-PCs were centrifuged, when reaching expiry day (7 days after collection), to remove plasma and platelet additive solution. The platelet pellet was re-suspended and concentrated in phosphate buffer saline, subjected to 3 freeze-thaw cycles (− 80 °C/37 °C) then centrifuged to remove cell debris. The supernatant was recovered and further purified, or not, by heat-treatment as in our previous investigations. The content in proteins and neurotrophic factors was determined and the toxicity and neuroprotective activity of the platelet lysates towards LUHMES cells or primary cortical/hippocampal neurons were assessed using ELISA, flow cytometry, cell viability and cytotoxicity assays and proteins analysis by Western blot. Results Platelet lysates contained the expected level of total proteins (ca. 7–14 mg/mL) and neurotrophic factors. Virally inactivated and heat-treated platelet lysates did not exert detectable toxic effects on neither Lund human mesencephalic dopaminergic LUHMES cell line nor primary neurons. When used at doses of 5 and 0.5%, they enhanced the expression of tyrosine hydroxylase and neuron-specific enolase in LUHMES cells and did not significantly impact synaptic protein expression in primary neurons, respectively. Furthermore, virally-inactivated platelet lysates tested were found to exert very strong neuroprotection effects on both LUHMES and primary neurons exposed to erastin, an inducer of ferroptosis cell death. Conclusion Outdated Intercept pathogen-reduced platelet concentrates can be used to prepare safe and highly neuroprotective human heat-treated platelet pellet lysates. These data open reassuring perspectives in the possibility to develop an effective biotherapy using virally-inactivated platelet lysates rich in functional neurotrophins for neuroregenerative medicine, and for further bio-industrial development. However, the data should be confirmed in animal models. Graphical abstract
Published: 2019

8. A homozygous MPZL2 deletion is associated with non syndromic hearing loss in a moroccan family

Author: Zied Riahi, Majida Charif, Christine Petit, Amale Bousfiha, Ghita Amalou, Aymane Bouzidi, Crystel Bonnet, Guy Lenaers, Soukaina Elrharchi, Abdelhamid Barakat, Mostafa Kandil, Institut Pasteur du Maroc, Réseau International des Instituts Pasteur (RIIP), MitoVasc - Physiopathologie Cardiovasculaire et Mitochondriale (MITOVASC), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Chouaib Doukkali (UCD), Génétique et Physiologie de l'Audition, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), ED 515 - Complexité du vivant, Sorbonne Université (SU), Faculté des Sciences Ben M'sik [Casablanca], Université Hassan II [Casablanca] (UH2MC), Université Mohamed 1 Oujda MAROC, Institut de l'Audition [Paris] (IDA), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Collège de France (CdF (institution)), This work was supported by Pasteur Institute of Morocco (IPM). This project was financially supported by CAMPUS FRANCE (PHC TOUBKAL 2019 (French-Morocco bilateral program) Grant Number: 39005ZL)., The authors are indebted to the family that contributed to this study and to the CAMPUS FRANCE French-Morocco PHC TOUBKAL 2019 bilateral program (Grant Number: 39005ZL)., Physiopathologie Cardiovasculaire et Mitochondriale (MITOVASC), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), and Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Hearing loss, MPZL2, [SDV]Life Sciences [q-bio], Deafness, 03 medical and health sciences, Consanguinity, 0302 clinical medicine, Tandem repeat, 030225 pediatrics, medicine, otorhinolaryngologic diseases, Humans, Allele, 030223 otorhinolaryngology, Hearing Loss, Genotyping, Gene, Exome sequencing, Genetics, business.industry, Haplotype, Homozygote, Non syndromic hearing loss, General Medicine, 3. Good health, Pedigree, Morocco, Otorhinolaryngology, Pediatrics, Perinatology and Child Health, Mutation (genetic algorithm), Mutation, medicine.symptom, business, Cell Adhesion Molecules
Abstract: International audience; Adhesion glycoproteins are implicated in the pathophysiology of hearing loss, the most frequent inherited sensory disorder, affecting 1 in 1000 new-borns. Exome sequencing of a consanguineous Moroccan patient with mild hearing loss identified for the first time in a North African family a single homozygous mutation c.72delA in MPZL2 gene, encoding the Myelin Protein Zero-Like 2, reported as causing deafness in two other populations. Variable tandem repeat genotyping of this family revealed that the c.72delA MPZL2 allele shared a common haplotype with Turkish and Dutch families. These results confirm the pathogenicity of this MPZL2 mutation in recessive mild to moderate non-syndromic deafness.
Published: 2021

9. Propargylimine in the laboratory and in space: Millimetre-wave spectroscopy and its first detection in the ISM

Author: Sergio Martín, Jesús Martín-Pintado, Jean-Claude Guillemin, Victor M. Rivilla, Barbara M. Giuliano, Luca Bizzocchi, Shaoshan Zeng, Andrea Pietropolli-Charmet, Paola Caselli, D. Prudenzano, M. A. Requena-Torres, Fernando Rico-Villas, Izaskun Jiménez-Serra, Bizzocchi L., Prudenzano D., Rivilla V.M., Pietropolli-Charmet A., Giuliano B.M., Caselli P., Martin-Pintado J., Jimenez-Serra I., Martin S., Requena-Torres M.A., Rico-Villas F., Zeng S., Guillemin J.-C., Max-Planck-Institut für Extraterrestrische Physik (MPE), Istituto Nazionale di Astrofisica (INAF), University of Ca’ Foscari [Venice, Italy], Spanish National Research Council (CSIC), European Southern Observatory (ESO), Joint ALMA Observatory (JAO), European Southern Observatory (ESO)-National Radio Astronomy Observatory (NRAO), Johns Hopkins University (JHU), Towson University [Towson, MD, United States], University of Maryland System, RIKEN - Institute of Physical and Chemical Research [Japon] (RIKEN), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), University Ca Foscari Venezia, CINECACINECA, Italy [HP10C2QE6F], European Union European Union (EU) [664931], Spanish FEDER [ESP2017-86582-C4-1-R], State Research Agency (AEI) Unidad de Excelencia Maria de Maeztu-Centro de Astrobiologia (INTA-CSIC) [MDM-2017-0737], program Physique et Chimie du Milieu Interstellaire (INSU-CNRS), Centre National d'Etudes Spatiales (CNES) Centre National D'etudes Spatiales, PHC Procope (CAMPUS France) [42516WC], DAADDeutscher Akademischer Austausch Dienst (DAAD) [57445281], RIKEN, European Project: 664931,H2020,H2020-MSCA-COFUND-2014,AstroFIt2(2015), Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737, Rico Villas, F. [0000-0002-5351-3497], Centre National D'Etudes Spatiales (CNES), European Research Council (ERC), Agencia Estatal de Investigación (AEI), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), CINECA, European Commission (EU), Spanish FEDER, PHC Procope (CAMPUS France), Deutscher Akademischer Austauschdienst (DAAD), and Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC
Subjects: Methods: laboratory: molecular, laboratory: molecular [Methods], ISM: clouds, ISM: molecules, Methods: observational, Molecular data, Techniques: spectroscopic, FOS: Physical sciences, Context (language use), Astrophysics, ISM: molecule, 01 natural sciences, Spectral line, spectroscopic [Techniques], Ab initio quantum chemistry methods, ISM: cloud, Distortion, 0103 physical sciences, [CHIM]Chemical Sciences, observational [Methods], Spectroscopy, 010303 astronomy & astrophysics, molecules [ISM], Settore CHIM/02 - Chimica Fisica, Physics, 010304 chemical physics, Spectrometer, Molecular cloud, Astronomy and Astrophysics, Astrophysics - Astrophysics of Galaxies, Interstellar medium, 13. Climate action, Space and Planetary Science, Astrophysics of Galaxies (astro-ph.GA), Atomic physics, clouds [ISM]
Abstract: Context. Small imines containing up to three carbon atoms are present in the interstellar medium (ISM). As alkynyl compounds are abundant in this medium, propargylimine (2-propyn-1-imine, HC C-CH =NH) thus represents a promising candidate for a new interstellar detection. Aims. The goal of the present work is to perform a comprehensive laboratory investigation of the rotational spectrum of propargylimine in its ground vibrational state in order to obtain a highly precise set of rest frequencies and to search for it in space. Methods. The rotational spectra of E and Z geometrical isomers of propargylimine have been recorded in the laboratory in the 83-500 GHz frequency interval. The measurements have been performed using a source-modulation millimetre-wave spectrometer equipped with a pyrolysis system for the production of unstable species. High-level ab initio calculations were performed to assist the analysis and to obtain reliable estimates for an extended set of spectroscopic quantities. We searched for propargylimine at 3 mm and 2 mm in the spectral survey of the quiescent giant molecular cloud G+0.693-0.027 located in the central molecular zone, close to the Galactic centre. Results. About 1000 rotational transitions have been recorded for the E-and Z-propargylimine, in the laboratory. These new data have enabled the determination of a very accurate set of spectroscopic parameters including rotational, quartic, and sextic centrifugal distortion constants. The improved spectral data allowed us to perform a successful search for this new imine in the G+0.693-0.027 molecular cloud. Eighteen lines of Z-propargylimine were detected at level >2.5σ, resulting in a column-density estimate of N = (0.24 ± 0.02) × 1014 cm-2. An upper limit was retrieved for the higher energy E isomer, which was not detected in the data. The fractional abundance (with respect to H2) derived for Z-propargylimine is 1.8 × 10-10. We discuss the possible formation routes by comparing the derived abundance with those measured in the source for possible chemical precursors., With funding from the Spanish government through the "María de Maeztu Unit of Excellence" accreditation (MDM-2017-0737)
Published: 2020

10. The shell matrix of the european thorny oyster, Spondylus gaederopus: microstructural and molecular characterization

Author: Jonathan Perrin, Marie Albéric, Beatrice Demarchi, Mathieu Thoury, Frédéric Marin, Jorune Sakalauskaite, Cédric Broussard, Jérôme Thomas, Laurent Plasseraud, Frédéric Jamme, Laffont, Rémi, Department of Life Sciences and Systems Biology [University of Turin], Università degli studi di Torino = University of Turin (UNITO), Biogéosciences [UMR 6282] (BGS), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] (ICMUB), Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut photonique d'analyse non-destructive européen des matériaux anciens (IPANEMA), Muséum national d'Histoire naturelle (MNHN)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Plateforme protéomique 3P5 [Institut Cochin] (3P5), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Work supported by the PHC Galilée programme (France, Italy), organised by Italo-French University (UIF/UFI) (project G18-464 / 39612SB) and Campus France, and support of the Campus France fund obtained from French embassy in Italy (BGF) and program Eiffel (France)., University of Turin, Biogéosciences [UMR 6282] [Dijon] (BGS), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC)
Subjects: Biomineralization, Proteomics, Proteome, Evolution, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Shell (structure), 03 medical and health sciences, Calcification, Physiologic, Animal Shells, Structural Biology, Animals, 14. Life underwater, 030304 developmental biology, Minerals, 0303 health sciences, biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Phylum, Chemistry, 030302 biochemistry & molecular biology, biology.organism_classification, Ostreidae, Liquid chromatography-tandem mass spectrometry, Shell biochemistry, Characterization (materials science), [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Spondylus, Evolutionary biology, Spondylus gaederopus, [SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology
Abstract: 17 pages; International audience; Molluscs, the largest marine phylum, display extraordinary shell diversity and sophisticated biomineral architectures. However, mineral-associated biomolecules involved in biomineralization are still poorly characterised.We report the first comprehensive structural and biomolecular study of Spondylus gaederopus, a pectinoid bivalve with a peculiar shell texture. Used since prehistoric times, this is the best-known shell of Europe’s cultural heritage. We find that Spondylus microstructure is very poor in mineral-bound organics, which are mostly intercrystalline and concentrated at the interface between structural layers.Using high-resolution liquid chromatography tandem mass spectrometry (LC-MS/MS) we characterized several shell protein fractions, isolated following different bleaching treatments. Several peptides were identified as well as six shell proteins, which display features and domains typically found in biomineralized tissues, including the prevalence of intrinsically disordered regions. It is very likely that these sequences only partially represent the full proteome of Spondylus, considering the lack of genomics data for this genus and the fact that most of the reconstructed peptides do not match with any known shell proteins, representing consequently lineage-specific sequences.This work sheds light onto the shell matrix involved in the biomineralization in spondylids. Our proteomics data suggest that Spondylus has evolved a shell-forming toolkit, distinct from that of other better studied pectinoids – fine-tuned to produce shell structures with high mechanical properties, while limited in organic content. This study therefore represents an important milestone for future studies on biomineralized skeletons and provides the first reference dataset for forthcoming molecular studies of Spondylus archaeological artifacts.
Published: 2020

11. Synthesis and biological evaluation of new naphthoquinones derivatives

Author: Isabelle Abrunhosa-Thomas, Pierre Chalard, Mohamed Akssira, Florence Caldefie-Chezet, Laetitia Delort, Caroline Decombat, El-Mahdi Ourhzif, Mostafa Khouili, Yves Troin, Institut de Chimie de Clermont-Ferrand (ICCF), Université Clermont Auvergne (UCA), Centre National de la Recherche Scientifique (CNRS), SIGMA Clermont, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Université Clermont Auvergne (UCA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Laboratoire de Chimie Organique et Analytique, FST Béni Mellal, Université Sultan Moulay Slimane, Laboratoire de chimie physique et chimie bio-organique, FST, Université Hassan II [Casablanca] (UH2MC), Institut de Chimie de Clermont-Ferrand - Clermont Auvergne (ICCF), Sigma CLERMONT (Sigma CLERMONT)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie, Bioorganique et analytique, SIGMA Clermont (SIGMA Clermont)-Institut de Chimie du CNRS (INC)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Unité de Nutrition Humaine (UNH), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Campus France (PHC Toubkal 17/55 Campus France) 36758 VANational Centre for Scientific and Technical Research (CNRST) of Morocco
Subjects: [SDV]Life Sciences [q-bio], Cytotoxicity, Cell, Antineoplastic Agents, [SDV.CAN]Life Sciences [q-bio]/Cancer, 010402 general chemistry, 01 natural sciences, Biochemistry, Structure-Activity Relationship, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Side chain, medicine, Humans, Human breast cancer cell line MCF-7, IC50, ComputingMilieux_MISCELLANEOUS, Cell Proliferation, Biological evaluation, Antitumor agents, Molecular Structure, 010405 organic chemistry, Organic Chemistry, Quinones, Combinatorial chemistry, Naphthoquinone, In vitro, 3. Good health, 0104 chemical sciences, medicine.anatomical_structure, chemistry, Active compound, MCF-7 Cells, Drug Screening Assays, Antitumor, Medicinal Chemistry, Naphthoquinones
Abstract: New substituted 1,4-naphthoquinones have been prepared in good overall yields through the naphthol route. The cytotoxicity of these compounds was tested in vitro on MCF-7 breast tumor cells. The most active compound 14 displayed an IC50 of 15μM. Objective: To investigate the cytotoxicity of new naphthoquinones derivatives on MCF-7 cells. Methods: Synthesis of new naphtoquinones derivatives and in vitro evaluation of their cytotoxicity on MCF-7 cells (rezasurin cell-based assay). Results: Starting from Ethyl 4-hydroxy-6,7-dimethoxy-2-naphthoate, four naphthoquinones were prepared and exhibited substantial cytotoxicity against MCF-7 cells. Conclusion: Preliminary studies of the structure-activity relationship have shown the influence of the structural parameters and, in particular, the nature of the naphthoquinone side chain.
Published: 2020

12. A Dynamic Transcriptional Analysis Reveals IL-6 Axis as a Prominent Mediator of Surgical Acute Response in Non-ischemic Mouse Heart

Author: Badawi, Sally, Paccalet, Alexandre, Harhous, Zeina, Pillot, Bruno, Augeul, Lionel, Van Coppenolle, Fabien, Lachuer, Joel, Kurdi, Mazen, Crola Da Silva, Claire, Ovize, Michel, Bidaux, Gabriel, Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), ProfileXpert, Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Cardiology, Hospices Civils de Lyon (HCL), National Council for Scientific Research and Lebanese University fellowship (CNRS-L) program, Eiffel Scholarship Program of Excellence of Campus France, Leducq Foundation [16CVD04], Association de Spécialisation et d'orientation Scientifique, French National Research Agency (ANR) [ANR-10-IBHU-004], INSERM through the 'young researcher funding', Cedar program of Campus France (2017-2018), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL)
Subjects: heart damage, transcriptomics, kinetical analysis, Physiology, inflammation, Physiology (medical), [SDV]Life Sciences [q-bio], interleukin 6, Original Research
Abstract: International audience; Background: Ischemic heart diseases are a major cause of death worldwide. Different animal models, including cardiac surgery, have been developed over time. Unfortunately, the surgery models have been reported to trigger an important inflammatory response that might be an effect modifier, where involved molecular processes have not been fully elucidated yet. Objective: We sought to perform a thorough characterization of the sham effect in the myocardium and identify the interfering inflammatory reaction in order to avoid misinterpretation of the data via systems biology approaches. Methods and Results: We combined a comprehensive analytical pipeline of mRNAseq dataset and systems biology analysis to characterize the acute phase response of mouse myocardium at 0 min, 45 min, and 24 h after surgery to better characterize the molecular processes inadvertently induced in sham animals. Our analysis showed that the surgical intervention induced 1209 differentially expressed transcripts (DETs). The clustering of positively co-regulated transcript modules at 45 min fingerprinted the activation of signalization pathways, while positively co-regulated genes at 24 h identified the recruitment of neutrophils and the differentiation of macrophages. In addition, we combined the prediction of transcription factors (TF) regulating DETs with proteinprotein interaction networks built from these TFs to predict the molecular network which have induced the DETs. By mean of this retro-analysis of processes upstream gene transcription, we revealed a major role of the Il-6 pathway and further confirmed a significant increase in circulating IL-6 at 45 min after surgery. Conclusion: This study suggests that a strong induction of the IL-6 axis occurs in sham animals over the first 24 h and leads to the induction of inflammation and tissues' homeostasis processes.
Published: 2019

13. Critical appraisal of STAT3 pattern in adult cardiomyocytes

Author: Emmanuelle Canet-Soulas, Bruno Pillot, Noelle Gallo Bona, Mazen Kurdi, George W. Booz, Sally Badawi, Lionel Augeul, Michel Ovize, Melanie Paillard, Gabriel Bidaux, Zeina Harhous, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Lebanese University [Beirut] (LU), University of Mississippi Medical Center (UMMC), This work was supported by the IHU OPeRa (ANR-10-IBHU-004) within the program 'Investissements d'Avenir' operated by the French National Research Agency (ANR). Zeina Harhous was supported by the 'Association de Specialisation et d'orientation Scientifique'. Sally Badawi was supported by the Council for Scientific Research and Lebanese University fellowship (CNRS-L) program and the Eiffel scholarship program of excellence of Campus France. We acknowledge the financial support from ITMO Cancer AVIESAN (Alliance Nationale pour les Sciences de la Vie et de la Santé, National Alliance for Life Sciences and Health) within the framework of the cancer plan for Orbitrap mass spectrometer founding., ANR-10-IBHU-0004,OPeRa,Organ ProtEction and ReplAcement(2010), bidaux, gabriel, Instituts Hospitalo-Universitaires B - Organ ProtEction and ReplAcement - - OPeRa2010 - ANR-10-IBHU-0004 - IBHU - VALID, Department of Cardiology, Hospices Civils de Lyon (HCL), Institut National de la Sante et de la Recherche Medicale (Inserm), Lebanese University, Cedar program of Campus France [37303QA 2017-2018], IHU OPeRa within the program 'Investissements d'Avenir' [ANR-10-IBHU-004], Association de Spécialisation et d'orientation Scientifique, Council for Scientific Research and Lebanese University fellowship (CNRS-L) program, Eiffel scholarship program of excellence of Campus France, ITMO Cancer AVIESAN (Alliance Nationale pour les Sciences de la Vie et de la Sante, National Alliance for Life Sciences and Health), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL)
Subjects: Atropine, Male, 0301 basic medicine, [SDV]Life Sciences [q-bio], 030204 cardiovascular system & hematology, Mitochondrion, Mass Spectrometry, Mitochondria, Heart, Oxidative Phosphorylation, Mice, Nitroglycerin, 0302 clinical medicine, Papaverine, Myocytes, Cardiac, 10. No inequality, STAT3, Mice, Knockout, Cardiomyocytes, biology, Chemistry, Ischemia-reperfusion, Brain, Aminophylline, Cell biology, Mitochondria, [SDV] Life Sciences [q-bio], Drug Combinations, T-tubules, medicine.anatomical_structure, Liver, Phenobarbital, Cell fractionation, Cardiology and Cardiovascular Medicine, Signal Transduction, STAT3 Transcription Factor, Cell type, Myocardial Reperfusion Injury, Cell Line, 03 medical and health sciences, medicine, Animals, Molecular Biology, Transcription factor, Endoplasmic reticulum, Subcellular distribution, Rats, Mice, Inbred C57BL, 030104 developmental biology, Mitochondrial permeability transition pore, biology.protein, Nucleus, Mitochondria associated membranes
Abstract: International audience; The signal transducer and activator of transcription 3, STAT3, transfers cellular signals from the plasma membrane to the nucleus, acting as a signaling molecule and a transcription factor. Reports proposed an additional non-canonical role of STAT3 that could regulate the activity of complexes I and II of the electron transport chain and the opening of the mitochondrial permeability transition pore (PTP) after ischemia-reperfusion in various cell types. The native expression of STAT3 in heart mitochondria, together with a direct versus an indirect transcriptional role in mitochondrial functions, have been recently questioned. The objective of the present study was to investigate the cellular distribution of STAT3 in mouse adult cardiomyocytes under basal and stress conditions, along with assessing its presence and activity in cardiac mitochondria using structural and functional approaches. The analysis of the spatial distribution of STAT3 signal in the cardiomyocytes interestingly showed that it is transversely distributed along the T-tubules and in the nucleus. This distribution was neither affected by hypoxia nor by hypoxia/re-oxygenation conditions. Focusing on the mitochondrial STAT3 localization, our results suggest that serine-phosphorylated STAT3 (PS727-STAT3) and total STAT3 are detected in crude but not in pure mitochondria of mouse adult cardiomyocytes, under basal and ischemia-reperfusion conditions. The inhibition of STAT3, with a pre-validated non-toxic Stattic dose, had no significant effects on mitochondrial respiration, but a weak effect on the calcium retention capacity. Overall, our results exclusively reveal a unique cellular distribution of STAT3 in mouse adult cardiomyocytes, along the Ttubules and in nucleus, under different conditions. They also challenge the expression and activity of STAT3 in mitochondria of these cells under basal conditions and following ischemia-reperfusion. In addition, our results underline technical methods, complemental to cell fractionation, to evaluate STAT3 roles during hypoxia-reoxygenation and at the interface between nucleus and endoplasmic reticulum.
Published: 2019

14. Update of an occupational asthma-specific job exposure matrix to assess exposure to 30 specific agents

Author: Le Moual, Nicole, Zock, Jan-Paul, Dumas, Orianne, Lytras, Theodore, Andersson, Eva, Lillienberg, Linnéa, Schlünssen, Vivi, Benke, Geza, Kromhout, Hans, One Health Chemisch, dIRAS RA-2, Dep IRAS, Vieillissement et Maladies chroniques : approches épidémiologique et de santé publique (VIMA), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Epidemiology & Pulmonology [Barcelona, Spain] (ISGlobal), Instituto de Salud Global - Institute For Global Health [Barcelona] (ISGlobal)-Centre de Recerca en Epidemiologia Ambiental - CREAL [Barcelona, Spain]-Universitat Pompeu Fabra [Barcelona] (UPF), Instituto de Salud Carlos III [Madrid] (ISC), Department of Occupational and Environmental Medicine [Gothenburg, Sweden], Sahlgrenska University Hospital [Gothenburg], Department of Public Health [Copenhagen, Denmark] (Danish Ramazzini Centre), Aarhus University [Aarhus]-The National Research Center for Work Environment [Copenhagen, Denmark], Monash Centre for Occupation and Environmental Health [Melbourne, Australia], School of Public Health and Preventive Medicine [Melbourne, Australia]-Monash University [Melbourne, Australia], Department of Environmental Epidemiology [Utrecht, the Netherlands], Utrecht University [Utrecht]-Institute for Risk Assessment Sciences [Utrecht, the Netherlands], PHC Van Gogh programme, EP-Nuffic, Campus France 33653RF (2015-2016), 7th programme-EU (Marie-Curie) [FP7/2007-2013], REA (PCOFUND-GA-2013-609102), PRESTIGE-2015-3-0029 - Campus France. ISGlobal is a member of the CERCA Programme, Generalitat de Catalunya., European Project: 609102,EC:FP7:PEOPLE,FP7-PEOPLE-2013-COFUND,PRESTIGE(2014), One Health Chemisch, dIRAS RA-2, Dep IRAS, Universitat Pompeu Fabra [Barcelona] (UPF)-Centre de Recerca en Epidemiologia Ambiental - CREAL [Barcelona, Spain]-Instituto de Salud Global - Institute For Global Health [Barcelona] (ISGlobal), Faraldo, Beatrice, and PRES Towards International Gain of Excellence - PRESTIGE - - EC:FP7:PEOPLE2014-09-01 - 2019-08-31 - 609102 - VALID
Subjects: medicine.medical_specialty, asthmagens, [SDV]Life Sciences [q-bio], Job-exposure matrix, Risk Assessment, 03 medical and health sciences, 0302 clinical medicine, Risk Factors, Occupational Exposure, Environmental health, Epidemiology, medicine, Humans, 030212 general & internal medicine, Asthma, Occupational, Occupations, occupational exposure assessment, Asthma, High probability, Sulfates, business.industry, Public Health, Environmental and Occupational Health, job-exposure matrix, International Standard Classification of Occupations, asthma, medicine.disease, [SDV] Life Sciences [q-bio], 030228 respiratory system, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Irritants, Solvents, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Occupational exposure, business, Occupational asthma
Abstract: ObjectivesWe aimed to update an asthmagen job exposure matrix (JEM) developed in the late 1990s. Main reasons were: the number of suspected and recognised asthmagens has since tripled; understanding of the aetiological role of irritants in asthma and methodological insights in application of JEMs have emerged in the period.MethodsFor each agent of the new occupational asthma-specific JEM (OAsJEM), a working group of three experts out of eight evaluated exposure for each International Standard Classification of Occupations, 1988 (ISCO-88) job code into three categories: ‘high’ (high probability of exposure and moderate-to-high intensity), ‘medium’ (low-to-moderate probability or low intensity) and ‘unexposed’. Within a working group, experts evaluated exposures independently from each other. If expert assessments were inconsistent the final decision was taken by consensus. Specificity was favoured over sensitivity, that is, jobs were classified with high exposure only if the probability of exposure was high and the intensity moderate-to-high. In the final review, all experts checked assigned exposures and proposed/improved recommendations for expert re-evaluation after default application of the JEM.ResultsThe OAsJEM covers exposures to 30 sensitisers/irritants, including 12 newly recognised, classified into seven broad groups. Initial agreement between the three experts was mostly fair to moderate (κ values 0.2–0.5). Out of 506 ISCO-88 codes, the majority was classified as unexposed (from 82.6% (organic solvents) to 99.8% (persulfates)) and a minority as ‘high-exposed’ (0.2% (persulfates) to 2.6% (organic solvents)).ConclusionsThe OAsJEM developed to improve occupational exposure assessment may improve evaluations of associations with asthma in epidemiological studies and contribute to assessment of the burden of work-related asthma.
Published: 2018

15. Bioaccessibility and bioavailability of environmental semi-volatile organic compounds via inhalation. A review of methods and models

Author: Philippe Glorennec, Gaëlle Raffy, Nathalie Bonvallot, Åsa Gustafsson, Corinne Mandin, Barbara Le Bot, Annette M. Krais, Olivier Ramalho, Wenjuan Wei, Centre Scientifique et Technique du Bâtiment (CSTB), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), EHESP-Irset (EHESP-Irset), École des Hautes Études en Santé Publique [EHESP] (EHESP), Département Santé Environnement Travail et Génie Sanitaire (DSETGS), Umeå University, Laboratoire d'étude et de recherche en environnement et santé (LERES), Lund University [Lund], FP7/2007-2013, FP7, Seventh Framework Programme, PRESTIGE-2015-3-0016, Campus France, Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Karolinska Institute, Département Méthodes quantitatives en santé publique (METIS), and This research received funding from the People Program (Marie Curie Actions) of the European Union Seventh Framework Programme (FP7/2007-2013) under REA grant agreement n. PCOFUND-GA-2013-609102, through the PRESTIGE Program coordinated by Campus France (PRESTIGE-2015-3-0016).
Subjects: 0301 basic medicine, Polychlorinated Dibenzodioxins, Future studies, Respiratory System, Biological Availability, Respiratory tract, 010501 environmental sciences, Models, Biological, 01 natural sciences, In vivo tests, Absorption, 03 medical and health sciences, Adverse health effect, Animals, Humans, Respiratory cycle, Polycyclic Aromatic Hydrocarbons, Deposition, lcsh:Environmental sciences, 0105 earth and related environmental sciences, General Environmental Science, lcsh:GE1-350, Inhalation exposure, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, Air Pollutants, Volatile Organic Compounds, Inhalation, Semi-volatile organic compounds SVOCs, Internal dose, 3. Good health, Bioavailability, 030104 developmental biology, 13. Climate action, Air Pollution, Indoor, Environmental chemistry, Environmental science, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Biological variability
Abstract: Semi-volatile organic compounds (SVOCs) present in indoor environments are known to cause adverse health effects through multiple routes of exposure. To assess the aggregate exposure, the bioaccessibility and bioavailability of SVOCs need to be determined.In this review, we discussed measurements of the bioaccessibility and bioavailability of SVOCs after inhalation. Published literature related to this issue is available for 2,3,7,8-tetrachlorodibenzo-p-dioxin and a few polycyclic aromatic hydrocarbons, such as benzo[a]pyrene and phenanthrene. Then, we reviewed common modeling approaches for the characterization of the gas- and particle-phase partitioning of SVOCs during inhalation. The models are based on mass transfer mechanisms as well as the structure of the respiratory system, using common computational techniques, such as computational fluid dynamics. However, the existing models are restricted to special conditions and cannot predict SVOC bioaccessibility and bioavailability in the whole respiratory system.The present review notes two main challenges for the estimation of SVOC bioaccessibility and bioavailability via inhalation in humans. First, in vitro and in vivo methods need to be developed and validated for a wide range of SVOCs. The in vitro methods should be validated with in vivo tests to evaluate human exposures to SVOCs in airborne particles. Second, modeling approaches for SVOCs need to consider the whole respiratory system. Alterations of the respiratory cycle period and human biological variability may be considered in future studies. Keywords: SVOCs, Respiratory tract, Absorption, Deposition, Inhalation exposure, Internal dose
Published: 2018

16. Electrochemistry, a tool to enhance self-purification in water systems while preventing the emission of noxious gases (greenhouse gases, H 2 S, NH 3 )

Author: Lucas Jobin, Philippe Namour, RiverLy (UR Riverly), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), The authors thank the French National Research Agency (ANR 14-OHRI-0016 El Hamico), the Field Observatory for Urban Water Management (OTHU – www.othu.org/ – FED 4161), the Rhône-Alpes Region ARC 3 Environment, ENVI-MED 2015 El Encobio and Campus France (PHC Utique Carthago 17G1006 and Imhotep Mareotis 37585QA) for their scientific and financial support., ANR 14-OHRI-0016,El Hamico,Gestion électrochimique de communautés microbiennes, Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), The authors thank the French National Research Agency (ANR 14-OHRI-0016 El Hamico), the Field Observatory for Urban Water Management (OTHU – www.othu.org/ – FED 4161), the Rhône-Alpes Region ARC 3 Environment, ENVI-MED 2015 El Encobio and Campus France (PHC Utique Carthago 17G1006 and Imhotep Mareotis 37585QA) for their scientific and financial support.Field Observatory for Urban Water Management (OTHU) : FED 4161Rhone-Alpes Region ARC 3 Environment, ENVI-MED 2015 El Encobio and Campus France : 17G1006, 37585QA, ANR-14-OHRI-0016,El Hamico,Gestion électrochimique de communautés microbiennes(2014), Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Field Observatory for Urban Water Management (OTHU) : FED 4161, and Rhone-Alpes Region ARC 3 Environment, ENVI-MED 2015 El Encobio and Campus France : 17G1006, 37585QA
Subjects: chemistry.chemical_classification, Microbial fuel cell, 02 engineering and technology, 010501 environmental sciences, Electron acceptor, 021001 nanoscience & nanotechnology, Electrochemistry, 01 natural sciences, 6. Clean water, Self purification, Analytical Chemistry, Anode, Bioremediation, chemistry, 13. Climate action, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Environmental chemistry, Greenhouse gas, Soil water, [SDE]Environmental Sciences, Environmental science, [CHIM]Chemical Sciences, 0210 nano-technology, 0105 earth and related environmental sciences
Abstract: International audience; This review describes the electrochemical control and stimulation of micro-organisms involved in the bioremediation of sediments and waterlogged soils. This emerging technology is derived from sedimentary microbial fuel cells, and consists of ensuring the respiration of aerobic microbial populations under anaerobic conditions by an anode as the terminal electron acceptor for electrons from microbial respiratory chains. This review describes the conceptual basis of electro-bioremediation, material used, and published implementations, from bench tests to some in-field set-ups.
Published: 2018

17. Rapid and Label-Free Electrochemical DNA Biosensor for Detecting Hepatitis A Virus

Author: Sara Viezzi, Sandra Mazerat, Marisa Manzano, Robert S. Marks, Jasmina Vidic, Università degli Studi di Udine - University of Udine [Italie], Nanyang Technological University (NTU), Université Paris Saclay (COmUE), Ben-Gurion University of the Negev (BGU), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), Campus France through the BioAsia Grant [35976PH], and Campus France through Galilee Grant [37184VG]
Subjects: 0209 industrial biotechnology, [SDV]Life Sciences [q-bio], viruses, Biosensing Techniques, 02 engineering and technology, Tripropylamine oxidation, 01 natural sciences, law.invention, 020901 industrial engineering & automation, Limit of Detection, law, Electrochemistry, Polymerase chain reaction, Chemistry, Hybridization probe, DNA–DNA hybridization, Nucleic Acid Hybridization, virus diseases, General Medicine, Hepatitis A, 021001 nanoscience & nanotechnology, 3. Good health, Hepatitis A virus detection, Cyclic voltammetry, DNA-electrochemical biosensor, AFM, DNA Probes, 0210 nano-technology, Biotechnology, Biophysics, Biomedical Engineering, DNA, Single-Stranded, lcsh:A, Nucleic acid thermodynamics, On-electrode hybridization, Complementary DNA, Humans, Detection limit, Base Sequence, 010401 analytical chemistry, Electrochemical Techniques, Molecular biology, digestive system diseases, 0104 chemical sciences, n/a, Food, DNA, Viral, Hepatitis A virus, lcsh:General Works, Biosensor
Abstract: International audience; Diagnostic systems that can deliver highly specific and sensitive detection of hepatitis A virus (HAV) in food and water are of particular interest in many fields including food safety, biosecurity and control of outbreaks. Our aim was the development of an electrochemical method based on DNA hybridization to detect HAV. A ssDNA probe specific for HAV (capture probe) was designed and tested on DNAs from various viral and bacterial samples using Nested-Reverse Transcription Polymerase Chain Reaction (nRT-PCR). To develop the electrochemical device, a disposable gold electrode was functionalized with the specific capture probe and tested on complementary ssDNA and on HAV cDNA. The DNA hybridization on the electrode was measured through the monitoring of the oxidative peak potential of the indicator tripropylamine by cyclic voltammetry. To prevent non-specific binding the gold surface was treated with 3% BSA before detection. High resolution atomic force microscopy (AFM) confirmed the efficiency of electrode functionalization and on-electrode hybridization. The proposed device showed a limit of detection of 0.65 pM for the complementary ssDNA and 6.94 fg/μL, for viral cDNA. For a comparison, nRT-PCR quantified the target HAV cDNA with a limit of detection of 6.4 fg/μL. The DNA-sensor developed can be adapted to a portable format to be adopted as an easy-to-use and low cost method for screening HAV in contaminated food and water. In addition, it can be useful for rapid control of HAV infections as it takes only a few minutes to provide the results.
Published: 2017

18. Low Temperature Synthesis and Phosphorescence of Methylcyanotriacetylene

Author: Claudine Crépin, Urszula Szczepaniak, Robert Kołos, Jean-Claude Guillemin, Marcin Gronowski, Institut des Sciences Moléculaires d'Orsay (ISMO), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Institute of Physical Chemistry, Polska Akademia Nauk = Polish Academy of Sciences (PAN), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Centre National d’Etudes Spatiales, Campus France, 2011/03/B/ST4/02763, Narodowe Centrum Nauki, Conseil National de la Recherche Scientifique, European Social Fund, 27752QB, Hubert Curien Partnership, Campus France, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Rare gas, [CHIM.ORGA]Chemical Sciences/Organic chemistry, 010402 general chemistry, Propyne, 01 natural sciences, Electron spectroscopy, 0104 chemical sciences, Characterization (materials science), [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, chemistry.chemical_compound, chemistry, 0103 physical sciences, Physical chemistry, Density functional theory, Physical and Theoretical Chemistry, Phosphorescence, 010303 astronomy & astrophysics
Abstract: International audience; This paper reports on UV-stimulated synthesis of methylcyanotriacetylene carried out in cryogenic rare gas matrixes via coupling of smaller precursors: propyne and cyanodiacetylene. The detection was possible due to the strong visible ã 3A' → X̃ 1A1 phosphorescence of CH3C7N, discovered in the course of this work. The ensuing measurements of electronic spectroscopy revealed the formally forbidden B̃ 1E-X̃ 1A1 system, as well as the allowed one Ẽ 1A1-X̃ 1A1, with origins at approximately 3.32 and 5.4 eV, respectively. It was also possible to revisit the spectroscopic characterization of cyanotriacetylene, HC7N, formed in parallel to the title photoproduct. Spectral assignments were assisted with a density functional theory study.
Published: 2017

19. Aging effect on microcirculation: A multiscale entropy approach on laser speckle contrast images

Author: Khalil, Adil, Humeau-Heurtier, Anne, Gascoin, L., Ábrahám, Pierre, Mahé, Guillaume, allard, christelle, Laboratoire Angevin de Recherche en Ingénierie des Systèmes (LARIS), Université d'Angers (UA), Biologie Neurovasculaire et Mitochondriale Intégrée (BNMI), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre d'Investigation Clinique [Rennes] (CIC), Université de Rennes (UR)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), We gratefully acknowledge the support provided by the ministry of higher education and scientific research in Iraq (Baghdad), the Embassy of France in Iraq (service of cooperation and cultural action in Baghdad), and Campus France Paris, France., Embassy of France in Iraq (service of cooperation and cultural action in Baghdad), Campus France Paris, France, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nantes Angers Le Mans, Biologie Neurovasculaire et Mitochondriale Intégrée, Centre National de la Recherche Scientifique (CNRS) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Université d'Angers (UA), Université de Rennes 1 (UR1) - Hôpital Pontchaillou - Institut National de la Santé et de la Recherche Médicale (INSERM), and Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Angers (UA)
Subjects: alzheimers-disease patients, Adult, skin, Aging, Entropy, sample entropy, Young Adult, endothelial function, Humans, microvascular blood-flow, ComputingMilieux_MISCELLANEOUS, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing, Aged, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Lasers, Microcirculation, age-related-changes, Optical Imaging, Equipment Design, Middle Aged, Forearm, Regional Blood Flow, perfusion signal, chaos theory, heart-rate-variability, complexity, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, Algorithms
Abstract: International audience; Purpose: It has long been known that age plays a crucial role in the deterioration of microvessels. The assessment of such deteriorations can be achieved by monitoring microvascular blood flow. Laser speckle contrast imaging (LSCI) is a powerful optical imaging tool that provides two-dimensional information on microvascular blood flow. The technique has recently been commercialized, and hence, few works discuss the postacquisition processing of laser speckle contrast images recorded in vivo. By applying entropy-based complexity measures to LSCI time series, we present herein the first attempt to study the effect of aging on microcirculation by measuring the complexity of microvascular signals over multiple time scales. Methods: Forearm skin microvascular blood flow was studied with LSCI in 18 healthy subjects. The subjects were subdivided into two age groups: younger (20-30 years old, n = 9) and older (50-68 years old, n = 9). To estimate age-dependent changes in microvascular blood flow, we applied three entropy-based complexity algorithms to LSCI time series. Results: The application of entropy-based complexity algorithms to LSCI time series can differentiate younger from older groups: the data fluctuations in the younger group have a significantly higher complexity than those obtained from the older group. Conclusions: The effect of aging on microcirculation can be estimated by using entropy-based complexity algorithms to LSCI time series. (C) 2016 American Association of Physicists in Medicine.
Published: 2016

20. A federation of simulations based on cellular automata in cyber-physical systems

Author: Hoang Van Tran, Bernard Pottier, Hiep Xuan Huynh, Vinh Cong Phan, Université de Cantho, Lab-STICC_UBO_CACS_MOCS, Université de Brest (UBO)-Laboratoire des sciences et techniques de l'information, de la communication et de la connaissance (Lab-STICC), École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Télécom Bretagne-Institut Brestois du Numérique et des Mathématiques (IBNM), Université de Brest (UBO)-Université européenne de Bretagne - European University of Brittany (UEB)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Télécom Bretagne-Institut Brestois du Numérique et des Mathématiques (IBNM), Université de Brest (UBO)-Université européenne de Bretagne - European University of Brittany (UEB)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des sciences et techniques de l'information, de la communication et de la connaissance (Lab-STICC), Université de Brest (UBO)-Université européenne de Bretagne - European University of Brittany (UEB)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Télécom Bretagne-Institut Brestois du Numérique et des Mathématiques (IBNM), Projet SAMES, MAE et Eiffel Campus France, Université de Can Tho, and UBO/CTU Cantho Projet SAMES, MAE et Eiffel Campus France
Subjects: Engineering, business.industry, Distributed computing, mixed simulations, Cyber-physical system, Physical system, 020207 software engineering, 02 engineering and technology, cellular automata (CA), [INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation, Cellular automaton, 020202 computer hardware & architecture, Variety (cybernetics), Fire spread, federation, lcsh:Technology (General), 0202 electrical engineering, electronic engineering, information engineering, high-level architecture (HLA), lcsh:T1-995, [INFO]Computer Science [cs], business, Cyber-physical system (CPS), Wireless sensor network, River pollution, Simulation
Abstract: International audience; In cyber-physical system (CPS), cooperation between a variety of computational and physical elements usually poses difficulties to current modelling and simulation tools. Although much research has proposed to address those challenges, most solutions do not completely cover uncertain interactions in CPS. In this paper, we present a new approach to federate simulations for CPS. A federation is a combination of, and coordination between simulations upon a standard of communication. In addition, a mixed simulation is defined as several parallel simulations federated in a common time progress. Such simulations run on the models of physical systems, which are built based on cellular automata theory. The experimental results are performed on a federation of three simulations of forest fire spread, river pollution diffusion and wireless sensor network. The obtained results can be utilized to observe and predict the behaviours of physical systems in their interactions.
Published: 2016
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21. Logarithmic bundles of deformed Weyl arrangements of type $A_2$

Author: Takuro Abe, Daniele Faenzi, Jean Vallès, Institute of Mathematics for Industry, Kyushu University, Kyushu University [Fukuoka], Institut de Mathématiques de Bourgogne [Dijon] ( IMB ), Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Mathématiques et de leurs Applications [Pau] ( LMAP ), Université de Pau et des Pays de l'Adour ( UPPA ) -Centre National de la Recherche Scientifique ( CNRS ), JSPS. Grant number : 24740012, Sakura Campus France project Geometrie, combinatoire et topologie des arrangements d'hyperplans, ANR-11-BS03-0011,GEOLMI,Geométrie et algèbre des inégalités matricielles linéaires avec applications en commande ( 2011 ), Department of Mechanical Engineering and Science, Kyoto University, Department of Mechanical Engineering and Science, Kyoto University [Kyoto]-Kyoto University [Kyoto], Algèbre et géométrie, Laboratoire de Mathématiques et de leurs Applications [Pau] (LMAP), Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS)-Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS), Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS), JSPS Grants-in-Aid for Young Scientists (B) No. 24740012 for Abe Takuro., Sakura Campus France project : Géométrie, and combinatoire et topologie des arrangements d'hyperplans for the three authors
Subjects: Pure mathematics, Logarithmic sheaves, Logarithm, MSC: 52C35, 14F05, 32S22, General Mathematics, 010102 general mathematics, Type (model theory), Weyl arrangements, 01 natural sciences, [ MATH.MATH-AG ] Mathematics [math]/Algebraic Geometry [math.AG], Mathematics - Algebraic Geometry, Computer Science::Graphics, Corollary, 0103 physical sciences, FOS: Mathematics, 010307 mathematical physics, Isomorphism, [MATH.MATH-AG]Mathematics [math]/Algebraic Geometry [math.AG], 0101 mathematics, Root systems, Line arrangements, MSC 52C35, 14F05, 32S22, Algebraic Geometry (math.AG), Mathematics
Abstract: We consider deformations of the Weyl arrangement of type $A_2$, which include the extended Shi and Catalan arrangements. These last ones are well-known to be free. We study their sheaves of logarithmic vector fields in all other cases, and show that they are Steiner bundles. Also, we determine explicitly their unstable lines. As a corollary, some counter-examples to the shift isomorphism problem are given., Comment: 13 pages
Published: 2016

22. The cradle of giants: insights into the origin of Scherotheca Bouché, 1972 (Lumbricidae, Crassiclitellata) with the descriptions of eight new species from Corsica, France

Author: Marchán, Daniel Fernández, Domínguez, Jorge, Hedde, Mickael, Decaëns, Thibaud, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Universidade de Vigo, Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), naturalist expedition, 'Our Planet Reviewed -Corsica 2019-2021'. This survey was organized by the Museum national d'Histoire naturelle (MNHN, Paris) in collaboration with and funded by the Collectivite de Corse and the Office francais de la Biodiversite (OFB)., Make Our Planet Great Again Postdoctoral grant from Campus France (mopga-postdoc-3-6111272103), and Xunta de Galicia (Conselleria de Cultura, Educacion e Ordenacion Universitaria. Secretaria Xeral de Universidades under grant ED431B 2019/038).
Subjects: new species, new combination, Annelida, Corsica, Biodiversity, Eumenescolex, Scherotheca, Crassiclitellata, Animalia, Clitellata, Animal Science and Zoology, Oligochaeta, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, molecular phylogeny, Lumbricidae, Ecology, Evolution, Behavior and Systematics, Taxonomy
Abstract: Copyright BOLD © 2014-2022; International audience; Scherotheca Bouché, 1972 is a highly diverse genus of Lumbricidae Rafinesque-Schmaltz, 1815, broadly distributed from Northern Italy and Corsica Island to the Pyrenees and Northeastern Iberian Peninsula. A recent survey of earthworm species diversity in Corsica resulted in the identification of 12 operational taxonomic units (OTU) within Scherotheca and the closely related genus Eumenescolex Qiu & Bouché, 1998, based on both morphological characters and COI barcodes. In this work, we complete molecular analyses to provide a dataset including four DNA markers (COI, 16S, 28S and ND1) and analyze it through Bayesian phylogeny reconstruction and barcode gap analysis. Our results highlight the existence of eight phylogenetically and morphologically distinct species, new for science, which we described herein as Scherotheca altarocca Marchán, Decäens & Domínguez, n. sp., Scherotheca marceli Marchán, Decäens & Domínguez, n. sp., Scherotheca darioi Marchán, Decäens & Domínguez, n. sp., Scherotheca boccaverghju Marchán, Decäens & Domínguez, n. sp., Scherotheca qiui Marchán, Decäens & Domínguez, n. sp., Scherotheca litoralis Marchán, Decäens & Domínguez, n. sp., Scherotheca capcorsana Marchán, Decäens & Domínguez, n. sp. and Scherotheca mausoleana Marchán, Decäens & Domínguez, n. sp. The diversity of the genus in Corsica has thus increased from four to 12 species. In addition, Eumenescolex emiliae Qiu & Bouché, 1998 appears phylogenetically nested within the Corsican Scherotheca, justifying amendment of the name to Scherotheca emiliae (Qiu & Bouché, 1998) n. comb. leading to 13 Corsican species in the genus. We further discuss diversity patterns, phylogenetic relationships and biogeographical history of the genus in the Corsica Island.
Published: 2023

23. CYTOTOXIC EVALUATION OF ORIGINAL PYRAZOLO[3,4-d]THIAZOLES AND PYRAZOLO[3,4-c]PYRAZOLES

Author: Ostache, Nicu-Cosmin, Pâris, Arnaud, Hiebel, Marie-Aude, Fînaru, Adriana-Luminita, Daniellou, Richard, Suzenet, Franck, Guillaumet, Gérald, Institut de Chimie Organique et Analytique (ICOA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), University of Bacău Vasile Alecsandri, ERASMUS grants and CNFIS-FDI-0151/2017_0087/2019_0130/2020_ 0123/2021), Francophone University Agency in Central and Eastern Europe (CE_124_FF_2016), Campus France (N 857352 B, N 902192 B), the Eiffel Scholarship Program of Excellence (N 870738 C), projects CHemBio (FEDER-FSE 2014-2020-EX003677), Techsab (FEDER-FSE 2014-2020- EX011313), the RTR Motivhealth (2019-00131403), University of Orléans, CNRS, France, ANR-11-LABX-0029,SYNORG,Synthèse Organique : des molécules au vivant(2011), and ANR-11-LABX-0018,IRON,Radiopharmaceutiques Innovants en Oncologie et Neurologie(2011)
Subjects: [CHIM.ORGA]Chemical Sciences/Organic chemistry, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, General Pharmacology, Toxicology and Pharmaceutics
Abstract: International audience; A concise and versatile synthetic strategy for the synthesis of original substituted heterobicycles was developed, relying on the use of aromatic hydrazines in one-pot condensations and Ullmann-type intramolecular cyclization sequences, as well as on chemoselective brominations and palladium-mediated cross-coupling reactions. In this study, the two series of novel heterobicycles were tested for their cytotoxic potential. The compounds were structurally characterized by nuclear magnetic resonance spectrometry (1H NMR and 13C NMR), infrared spectroscopy (IR) and high-resolution mass spectrometry (HRMS). The IC50 values of these compounds on five tumour cell lines (A-549, HS-683, MCF-7, SK-MEL-28 and B16-F1) were obtained by MTT colorimetric assay. Six of the compounds exhibited excellent activity compared to 5-fluorouracil against two of the five cell lines tested, with IC50 values ranging from 0.1 to 10 μM.
Published: 2022

24. Development of a Chitosan/Nickel Phthalocyanine Composite Based Conductometric Micro‐sensor for Methanol Detection

Author: Ibrahim Musa, Guy Raffin, Marie Hangouet, Marie Martin, Albert Alcacer, Nadia Zine, Francesca Bellagambi, Nicole Jaffrezic‐Renault, Abdelhamid Errachid, Micro & Nanobiotechnologies, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), spectroscopies vibrationnelles, Centro Nacional de Microelectronica [Spain] (CNM), The authors acknowledge the financial support of the EU H2020 WIDESPREAD Program entitled Bionanosens grant agreement # 951887 and France Embassy of Nigeria, and Kano state government of Nigeria, through CAMPUS FRANCE under the MoU agreement KN-FR scholarship., and European Project: 951887,Bionanosens
Subjects: [SDV]Life Sciences [q-bio], Electrochemistry, [CHIM]Chemical Sciences, Analytical Chemistry
Abstract: International audience; Thin-film composite of chitosan/nickel phthalocyanine (NiPc) was electrochemically deposited on the fingers of interdigitated gold electrodes, applying chronoamperometric polymerization technique. The presence of crystallized NiPc in the chitosan was confirmed by EDX and FTIR analysis. Acetone, ethanol, and methanol gas-sensing properties of the films prepared at optimum conditions were studied at atmospheric temperature, through differential measurements at an optimized frequency of 10 kHz, using a lock-in amplifier. The conductometric sensor presents the highest sensitivity of 60.2 μS.cm−1(v/v) for methanol and 700 ppm as the limit of detection. For validation, the methanol content of a commercial rubbing alcohol was determined.
Published: 2022

25. Photocatalytic Performance of Perovskite and Metal–Organic Framework Hybrid Material for the Reduction of N2 to Ammonia

Author: Masoumeh Chamack, Madjid Ifires, Sayed Ali Akbar Razavi, Ali Morsali, Ahmed Addad, Afsanehsadat Larimi, Sabine Szunerits, Rabah Boukherroub, Tarbiat Modares University [Tehran], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Unité Matériaux et Transformations - UMR 8207 (UMET), Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), and National Centre for Scientific Research (CNRS), Center for International Scientific Studies and Collaboration (GUNDISHAPUR 2019, Project No. 261), The University of Lille, the Hauts-de-France region, Tarbiat Modares University (TMU), Campus France (PHC GUNDISHAPUR 2019, Project No. 42965PK) and Niroo Research Institute (NRI) are gratefully acknowledged for financial support.
Subjects: [PHYS]Physics [physics], Inorganic Chemistry, X-ray photoelectron spectroscopy, [SPI]Engineering Sciences [physics], Nitrogen, Photocatalysts, Metal organic frameworks, Physical and Theoretical Chemistry, Composites
Abstract: International audience; The orthorhombic phase of KNbO3 perovskite has been applied for nitrogen (N-2) photoreduction to ammonia (NH3). However, this material suffers from a low surface area and low ammonia production efficiency under UV light irradiation. To eliminate these barriers, we used a metal-organic framework (MOF), named as TMU-5 ([Zn(OBA)(BPDH)(0.5)](n).1.5DMF, where H(2)OBA = 4,4'-oxybis(benzoic acid) and BPDH = 2,5-bis(4-pyridyl)-3,4-diaza-2,4-hexadiene), for the synthesis of the KNbO3@TMU-5 hybrid material. KNbO3@TMU-5 achieved a NH3 production rate of 39.9 mu mol.L-1.h(-1).g(-1) upon UV light irradiation, as compared to 20.5 mu mol.L-1.h(-1).g(-1) recorded for KNbO3 under similar experimental conditions. Using different characterization techniques especially gas adsorption, cyclic voltammetry, X-ray photoelectron spectroscopy, photocurrent measurements, and Fourier transform infrared spectroscopy, it has been found that the higher photoactivity of KNbO3@TMU-5 in ammonia production is due to its higher surface area, higher electron-hole separation efficiency, and higher density of negative charges on Nb sites. This work shows that hybridization of conventional semiconductors (SCs) with photoactive MOFs can improve the photoactivity of the SC@MOF hybrid material in different reactions, especially kinetically complex reactions like photoconversion of nitrogen to ammonia.
Published: 2022

26. Atmospheric dispersion modelling of gaseous emissions from Beirutinternational airport activities

Author: Mokalled, Tharwat, Le Calvé, Stéphane, Badaro-Saliba, Nada, Abboud, Maher, Zaarour, Rita, Farah, Wehbeh, Adjizian-Gérard, Jocelyne, Institut de chimie et procédés pour l'énergie, l'environnement et la santé (ICPEES), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Saint-Joseph de Beyrouth (USJ), Unité de Recherche Environnement, Génomique Fonctionnelle et Études Mathématiques [Beyrouth] (UR-EGFEM), This research was funded by the Research Council at Saint Joseph University and supported by Strasbourg University and Campus France., and Le Calvé, Stéphane
Subjects: [SDU.OCEAN]Sciences of the Universe [physics]/Ocean, Atmosphere, volatile organic compound, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, Beirut, nitrogen dioxide, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDE.IE]Environmental Sciences/Environmental Engineering, [SDU.OCEAN] Sciences of the Universe [physics]/Ocean, Atmosphere, aviation emission, atmospheric dispersion modelling system (ADMS), [SDE.IE] Environmental Sciences/Environmental Engineering, General Environmental Science
Abstract: The projected increase of civil aviation activity, the degradation of air quality and the location of Beirut Airport embedded in a very urbanized area, in addition to the special geography and topography surrounding the airport which plays a significant role in drawing emissions to larger distances, demanded anassessment of the spatial impact of the airport activities on the air quality of Beirut and its suburbs. This is the first study in the Middle East region that model pollutant concentrations resulting from an international airport's activities using an advanced atmospheric dispersion modelling system in a country with no data. This followed validation campaigns showing very strong correlations (r = 0.85) at validation sites as close as possible to emission sources. The modelling results showed extremely high NO2 concentrations within the airport vicinity, i.e., up to 110 μg∙m-3 (which is greater than the World Health Organization annual guidelines) posing a health hazard to the workers in the ramp. The major contribution of Beirut–Rafic Hariri International Airport to the degradation of air quality was in the airport vicinity; however, it extended to Beirut and its suburbs in addition to affecting the seashore area due to emissions along the aircraft trajectory; this isan aspect rarely considered in previous studies. On the other hand, elevated volatile organic compound levels were observed near the fuel tanks and at the aerodrome center. This study provides (ⅰ) a methodology to assess pollutant concentrations resulting from airport emissions through the use of an advanced dispersion model in a country with no data; and (ⅱ) a tool for policy makers to better understand the contribution of the airport's operations to national pollutant emissions, which is vital for mitigation strategies and health impact assessments.
Published: 2022

27. New insights into the crystal structure of UTE0.25Ge2=U4TGe8 (TE = Ru, Os) and ferromagnetic properties of the Os-bearing uranium germanide

Author: Graziella Guy, Maria Szlawska, Carmelo Prestipino, Vincent Dorcet, Valérie Demange, Pierre Fertey, Dariusz Kaczorowski, Mathieu Pasturel, Adam Pikul, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Polska Akademia Nauk = Polish Academy of Sciences (PAN), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Polish National Agency for Academic Exchange NAWA (Warsaw, Poland) [PPN/BFR/2020/1/00022/U/00001], Campus France (Paris, France) [46871QL], PHC Polonium program, [ISCR-UMR6226], and [ScanMat-UAR2025]
Subjects: Inorganic Chemistry, Magnetic and related properties, Materials Chemistry, Ceramics and Composites, Ferromagnetism, [CHIM.CRIS]Chemical Sciences/Cristallography, Physical and Theoretical Chemistry, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, Condensed Matter Physics, Ordered vacancies, Electron and synchrotron X-ray diffraction, Electronic, Optical and Magnetic Materials, Uranium germanides
Abstract: International audience; A single crystal of the compound previously described as "URu0.25Ge2" was studied by means of transmission electron microscopy and synchrotron X-ray diffraction (XRD) measurements. The experiments revealed that the deficient ruthenium atoms order to a superstructure of the CeNiSi2 structure-type with space group P21/n (no. 14) and cell parameters a' = 5.7422(7) angstrom, b' = 15.931(2) angstrom, c' =11.4903(9) angstrom and beta' = 90.755(8)degrees, corresponding to the Tb4FeGe8-type structure. In addition, a new isostructural compound, U4OsGe8, was synthesized and charac-terized by powder X-ray diffraction, electron diffraction, DC magnetization, AC susceptibility, electrical resistivity and specific heat measurements. The experiments showed that it crystallizes with the monoclinic, ordered P21/n unit cell with lattice parameters a' = 5.7438(1) angstrom, b' = 15.9355(1) angstrom, c' = 11.4586(1) angstrom and beta' = 90.681(1)degrees, and orders ferromagnetically at about 54 K, which is clearly seen in all the physical properties studied. Moreover, its electrical resistivity was found to exhibit features characteristic of semimetals, and the electron contribution to the specific heat is moderately enhanced, as in all other known phases of the UTE1-xGe2 (with TE = transition element) family of compounds.
Published: 2023

28. Catch-All No More: Integrative Systematic Revision of the Genus Allolobophora Eisen, 1874 (Crassiclitellata, Lumbricidae) with the Description of Two New Relict Earthworm Genera

Author: Alejandro Martínez Navarro, Sergio Jiménez Pinadero, Thibaud Decaëns, Mickaël Hedde, Marta Novo, Dolores Trigo, Daniel Fernández Marchán, Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Department of Biodiversity, Ecology and Evolution [Madrid], Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), subproject Fauna Ibérica XII. Oligochaeta, Lumbricoidea: Lumbricidae, Hormogastridae #PGC2018-095851-B-C66 from the Spanish Ministry of Sciences, Innovation and Universities., María Zambrano Postdoctoral Grant from the Spanish Ministry of Sciences, Innovation and Universities, and Make Our Planet Great Again Postdoctoral grant from Campus France
Subjects: Article Subject, Genetics, Animal Science and Zoology, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, Molecular Biology, Ecology, Evolution, Behavior and Systematics
Abstract: International audience; The taxonomy of earthworms has been riddled by instability, lack of systematically useful characters, and lax diagnoses of some genera. This has led to the use of some genera such as Allolobophora Eisen, 1874 as taxonomic wastebaskets, blurring their evolution and biogeographical history. The implementation of molecular techniques has revolutionized the systematics of the genus; however, some of its species have not been previously included in molecular phylogenetic analyses. Thus, the molecular markers COI, 16S, ND1, 12S, and 28S were sequenced for six endemic species including several taxa of Allolobophora and Aporrectodea Örely, 1885 (another related catch-all genus). Phylogenetic relationships determined by Bayesian inference and maximum likelihood analyses support the status of two of the six taxa examined (Allolobophora burgondiae Bouché, 1972 and Aporrectodea icterica Savigny, 1826) as part of Allolobophora sensu stricto and a presumed synonymy between Allolobophora and Heraclescolex Qiu and Bouché, 1998. Branch lengths and average pairwise genetic distances support the transfer of Allolobophora satchelli Bouché, 1972 to the genus Panoniona Mršić and Šapkarev, 1988 and the emergence of two new genera, Heraultia gen. nov. and Vosgesia gen. nov., endemic to France, hosting Allolobophora tiginosa Bouché, 1972 and Allolobophora zicsii Bouché, 1972, respectively. The aforementioned changes of status and the diagnosis for Heraultia and Vosgesia are presented. These results provided more evolutionarily and biogeographically coherent earthworm groups and highlighted that the Maghreb and the area around the Alps are potential key locations for the diversification of Allolobophora and several lineages of Lumbricidae.
Published: 2023

29. Machine Learning Based Fast QTMTT Partitioning Strategy for VVenC Encoder in Intra Coding

Author: Ibrahim Taabane, Daniel Menard, Anass Mansouri, Ali Ahaitouf, Institut d'Électronique et des Technologies du numéRique (IETR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Université Sidi Mohamed Ben Abdellah (USMBA), Campus France, Moroccan National Center of Scientific and Technology Research (CNRST), and 45988WG
Subjects: VVenC, Computer Networks and Communications, QTMTT, LightGBM, video coding standard, VVC, intra coding, machine learning, [SPI]Engineering Sciences [physics], Hardware and Architecture, Control and Systems Engineering, Signal Processing, Electrical and Electronic Engineering
Abstract: International audience; The newest video compression standard, Versatile Video Coding (VVC), was finalized in July 2020 by the Joint Video Experts Team (JVET). Its main goal is to reduce the bitrate by 50% over its predecessor video coding standard, the High Efficiency Video Coding (HEVC). Due to the new advanced tools and features included in VVC, it actually provides high coding performances-for instance, the Quad Tree with nested Multi-Type Tree (QTMTT) involved in the partitioning block. Furthermore, VVC introduces various techniques that allow for superior performance compared to HEVC, but with an increase in the computational complexity. To tackle this complexity, a fast Coding Unit partition algorithm based on machine learning for the intra configuration in VVC is proposed in this work. The proposed algorithm is formed by five binary Light Gradient Boosting Machine (LightGBM) classifiers, which can directly predict the most probable split mode for each coding unit without passing through the exhaustive process known as Rate Distortion Optimization (RDO). These LightGBM classifiers were offline trained on a large dataset; then, they were embedded on the optimized implementation of VVC known as VVenC. The results of our experiment show that our proposed approach has good trade-offs in terms of time-saving and coding efficiency. Depending on the preset chosen, our approach achieves an average time savings of 30.21% to 82.46% compared to the VVenC encoder anchor, and a Bjontegaard Delta Bitrate (BDBR) increase of 0.67% to 3.01%, respectively.
Published: 2023

30. Peroxisomal defects in microglial cells induce a disease-associated microglial signature

Author: Quentin Raas, Ali Tawbeh, Mounia Tahri-Joutey, Catherine Gondcaille, Céline Keime, Romain Kaiser, Doriane Trompier, Boubker Nasser, Valerio Leoni, Emma Bellanger, Maud Boussand, Yannick Hamon, Alexandre Benani, Francesca Di Cara, Caroline Truntzer, Mustapha Cherkaoui-Malki, Pierre Andreoletti, Stéphane Savary, Raas, Q, Tawbeh, A, Tahri-Joutey, M, Gondcaille, C, Keime, C, Kaiser, R, Trompier, D, Nasser, B, Leoni, V, Bellanger, E, Boussand, M, Hamon, Y, Benani, A, Di Cara, F, Truntzer, C, Cherkaoui-Malki, M, Andreoletti, P, Savary, S, Laboratoire Bio-PeroxIL. Biochimie du peroxysome, inflammation et métabolisme lipidique [Dijon] (BIO-PEROXIL), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Faculty of Science and Technology, University Hassan I, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Milano-Bicocca = University of Milano-Bicocca (UNIMIB), Mi-mAbs (C/O CIML), Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Bourgogne Franche-Comté [COMUE] (UBFC), Dalhousie University [Halifax], Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER, We warmly acknowledge the Fondation Maladies Rares which supported our transcriptomic analysis project (GenOmics: High throughput sequencing and rare diseases, call 2017-20170615). Sequencing was performed by the GenomEast platform, a member of the 'France Génomique' consortium (ANR-10-INBS-0009). We would like to acknowledge the SATT Sayens of Dijon, the CHU of Dijon (project CRBSEP-EMATSEP grant), the Canadian New Frontiers Research Funds Exploration (NFRF-E 19-00007), and the regional council of Bourgogne Franche-Comté (Project PERSIL 2019) for their support. We are also grateful to networking support by the COST Action CA 16,112 NutRedOx (Personalized Nutrition in aging society: redox control of major age-related diseases), supported by COST (European Cooperation in Science and Technology). We thank the Centre d’Immunologie de Marseille-Luminy (CIML) flow cytometry facility. This work was supported by institutional grants from INSERM, CNRS and Aix-Marseille University to the CIML and program grant from the French National Research Agency (ANR-17-CE15-0032). The project leading to this publication has received funding from Excellence Initiative of Aix-Marseille University—A*MIDEX, a French 'Investissements d’Avenir' program. The laboratory BioPeroxIL was funded by the Ministère de l’Education Nationale et de l’Enseignement Supérieur et de la Recherche (France) and by the University of Bourgogne. MT-J was funded by CNRST (PhD excellence grant number: 17UHP2019, Morocco) and by the Action Intégrée of the Comité Mixte Inter-universitaire Franco-Marocain (n° TBK 19/92 n° Campus France: 41501RJ) from the PHC Toubkal program, Ministère des Affaires Étrangères., ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), and ANR-17-CE15-0032,NanoGammaR,Comprendre comment la dynamique membranaire du récepteur à l'IFN-gamma à l'échelle nanométrique contrôle les réponses immunes médiées par l'IFN-gamma(2017)
Subjects: Cellular and Molecular Neuroscience, autophagy, [SCCO.NEUR]Cognitive science/Neuroscience, lipid metabolism, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], adrenoleukodystrophy (X-ALD), lysosome, microglia, peroxisome, Molecular Biology
Abstract: International audience; Microglial cells ensure essential roles in brain homeostasis. In pathological condition, microglia adopt a common signature, called disease-associated microglial (DAM) signature, characterized by the loss of homeostatic genes and the induction of disease-associated genes. In X-linked adrenoleukodystrophy (X-ALD), the most common peroxisomal disease, microglial defect has been shown to precede myelin degradation and may actively contribute to the neurodegenerative process. We previously established BV-2 microglial cell models bearing mutations in peroxisomal genes that recapitulate some of the hallmarks of the peroxisomal β-oxidation defects such as very long-chain fatty acid (VLCFA) accumulation. In these cell lines, we used RNA-sequencing and identified large-scale reprogramming for genes involved in lipid metabolism, immune response, cell signaling, lysosome and autophagy, as well as a DAM-like signature. We highlighted cholesterol accumulation in plasma membranes and observed autophagy patterns in the cell mutants. We confirmed the upregulation or downregulation at the protein level for a few selected genes that mostly corroborated our observations and clearly demonstrated increased expression and secretion of DAM proteins in the BV-2 mutant cells. In conclusion, the peroxisomal defects in microglial cells not only impact on VLCFA metabolism but also force microglial cells to adopt a pathological phenotype likely representing a key contributor to the pathogenesis of peroxisomal disorders.
Published: 2023

31. Enhancement of microbial fuel cell efficiency by incorporation of graphene oxide and functionalized graphene oxide in sulfonated polyethersulfone membrane

Author: Anthony Szymczyk, Mehri Shabani, Ahmad Rahimpour, Maxime Pontié, Hanxiao Guo, Mostafa Rahimnejad, Habibollah Younesi, Tarbiat Modares University [Tehran], ESAIP, Université d'Angers (UA), Babol Noshirvani University of Technology, Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Iran National Science Foundation, INSF: 97014305, IG-39804, Campus France, Tarbiat Modares University, TMU, Universite Angers, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Microbial fuel cell, Materials science, Oxide, 02 engineering and technology, Conductivity, 010402 general chemistry, 01 natural sciences, law.invention, chemistry.chemical_compound, law, Functionalized GO, [CHIM]Chemical Sciences, Electricity generation, Renewable Energy, Sustainability and the Environment, Graphene, Proton exchange membranes, 021001 nanoscience & nanotechnology, Polyelectrolyte, 0104 chemical sciences, Membrane, chemistry, Chemical engineering, 0210 nano-technology, Selectivity, Faraday efficiency
Abstract: International audience; The present work examines, for the first time, the use of thiolated graphene oxide (TGO), in polyelectrolyte composite membranes as an effective approach to enhance the MFC performance. A new composite membrane based on a sulfonated polyethersulfone (SPES) hybrid with GO, sulfonated GO (SGO), and TGO was fabricated and assessed in MFC. The blend membranes were characterized with various techniques. The sulfhydryl (-SH) and sulfonic (-SO3H) groups enhanced the proton selectivity of the membrane and MFC performance. The MFC using the SPES/SGO1.8% composite membrane generated a power density of 66.4 mW/m2 which was double that produced by MFC using Nafion117 membrane in batch mode which lasted for 8 days. The SPES/SGO membrane was more selective towards H+ rather than other cations (K+, Na+, and Li+). This was also confirmed by the results of proton conductivity analysis, as the SPES/SGO1.8% membranes showed a value of 1.42 mS/cm which was higher than Nafion117 (1.3 mS/cm), SPES/TGO1.8% (1.25 mS/cm), SPES/GO1.8% (0.56 mS/cm), and SPES (0.32 mS/cm). The higher COD removal and coulombic efficiency were obtained in MFC with SPES/SGO membranes. In conclusion, it is our view that the new SPES/SGO and SPES/TGO membranes can be applied favorably in dual-chamber MFCs meeting their needs. © 2021 Elsevier Ltd
Published: 2021

32. Improving Tokenization Expressiveness With Pitch Intervals

Author: Kermarec, Mathieu, Bigo, Louis, Keller, Mikaela, Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189 (CRIStAL), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Machine Learning in Information Networks (MAGNET), Inria Lille - Nord Europe, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189 (CRIStAL), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Algomus, Modélisation, Information et Systèmes - UR UPJV 4290 (MIS), Université de Picardie Jules Verne (UPJV)-Université de Picardie Jules Verne (UPJV)-Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189 (CRIStAL), The authors are grateful to the Algomus and Magnet teams for fruitful discussions. This work is supported by a special interdisciplinary funding (AIT) from the CRIStAL laboratory and the Merlion PHC Music Language Processing N°48304SM funded by Campus France., Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189 [CRIStAL], Machine Learning in Information Networks [MAGNET], and Bigo, Louis
Subjects: [SHS.MUSIQ] Humanities and Social Sciences/Musicology and performing arts, [INFO.INFO-AI] Computer Science [cs]/Artificial Intelligence [cs.AI], [SHS.MUSIQ]Humanities and Social Sciences/Musicology and performing arts, [INFO.INFO-CL] Computer Science [cs]/Computation and Language [cs.CL], [INFO.INFO-CL]Computer Science [cs]/Computation and Language [cs.CL], [INFO.INFO-AI]Computer Science [cs]/Artificial Intelligence [cs.AI]
Abstract: International audience; Training sequence models such as transformers with symbolic music requires a representation of music as sequences of atomic elements called tokens. State-of-the-art music tokenizations encode pitch values explicitly, which complicates the ability of a machine learning model to generalize musical knowledge at different keys. We propose tracks for a tokenization encoding pitch intervals rather than pitch values, resulting in transposition invariant representations. The musical expressiveness of this new tokenization is evaluated through two MIR classification tasks: composer classification and end of phrase detection. We release publicly the code produced in this research.
Published: 2022

33. An electroencephalography connectome predictive model of major depressive disorder severity

Author: Kabbara, Aya, Robert, Gabriel, Khalil, Mohamad, Verin, Marc, Benquet, Pascal, Hassan, Mahmoud, Lebanese Association for Scientific Research [Tripoli] (LASeR), MINDig, Centre Hospitalier Guillaume Régnier [Rennes], Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Comportement et noyaux gris centraux - EA 4712, Université de Rennes (UR)-Institut des Neurosciences Cliniques de Rennes (INCR), emlyon business school (EM), Lebanese University [Beirut] (LU), Ecole Doctorale des Sciences et de la Technologie (EDST), Azm Center for research in biotechnology and its applications [TRIPOLI], Université Libanaise, CHU Pontchaillou [Rennes], Laboratoire Traitement du Signal et de l'Image (LTSI), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Reykjavík University, robert, gabriel, Comportement et noyaux gris centraux = Behavior and Basal Ganglia [Rennes], Université de Rennes (UR)-Université européenne de Bretagne - European University of Brittany (UEB)-CHU Pontchaillou [Rennes]-Institut des Neurosciences Cliniques de Rennes (INCR), This work was financed by the Rennes University, the Institute of Clinical Neuroscience of Rennes (Projects named EEGCog and EEGNET3). This work was also financed by the AZM and SAADE Association, Tripoli, Lebanon and by the National Council for Scientific Research (CNRS) in Lebanon. Authors would like to thank Campus France, Programme Hubert Curien CEDRE (PROJET N° 42257YA) and the Lebanese Association for Scientific Research (LASER) for their support, HAL UR1, Admin, Université de Rennes (UR)-Institut des Neurosciences Cliniques de Rennes = Institute of Clinical Neurosciences of Rennes (INCR), and Université de Rennes (UR)-Université européenne de Bretagne - European University of Brittany (UEB)-CHU Pontchaillou [Rennes]-Institut des Neurosciences Cliniques de Rennes = Institute of Clinical Neurosciences of Rennes (INCR)
Subjects: [SDV.IB] Life Sciences [q-bio]/Bioengineering, Depressive Disorder, Major, Multidisciplinary, [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, [SDV]Life Sciences [q-bio], [SDV.MHEP.PSM] Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, [SDV.NEU.PC] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, Brain, Electroencephalography, [STAT] Statistics [stat], Machine Learning, [STAT]Statistics [stat], [SDV] Life Sciences [q-bio], FOS: Biological sciences, Quantitative Biology - Neurons and Cognition, [SDV.MHEP.PSM]Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, Connectome, Humans, Neurons and Cognition (q-bio.NC), [SDV.IB]Life Sciences [q-bio]/Bioengineering, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing
Abstract: Emerging evidence showed that major depressive disorder (MDD) is associated with disruptions of brain structural and functional networks, rather than impairment of isolated brain region. Thus, connectome-based models capable of predicting the depression severity at the individual level can be clinically useful. Here, we applied a machine-learning approach to predict the severity of depression using resting-state networks derived from source-reconstructed Electroencephalography (EEG) signals. Using regression models and three independent EEG datasets (N = 328), we tested whether resting state functional connectivity could predict individual depression score. On the first dataset, results showed that individuals scores could be reasonably predicted (r = 0.6, p = 4 × 10–18) using intrinsic functional connectivity in the EEG alpha band (8–13 Hz). In particular, the brain regions which contributed the most to the predictive network belong to the default mode network. We further tested the predictive potential of the established model by conducting two external validations on (N1 = 53, N2 = 154). Results showed statistically significant correlations between the predicted and the measured depression scale scores (r1 = 0.52, r2 = 0.44, p
Published: 2022

34. ENSO diversity shows robust decadal variations that must be captured for accurate future projections

Author: Bastien Dieppois, Antonietta Capotondi, Benjamin Pohl, Kwok Pan Chun, Jonathan Eden, Paul-Arthur MOnerie, Centre for Agroecology, Water and Resilience, Coventry University, Department of Oceanography [Cape Town], University of Cape Town, Cooperative Institute for Research in Environmental Sciences (CIRES), University of Colorado [Boulder]-National Oceanic and Atmospheric Administration (NOAA), NOAA Physical Sciences Laboratory (PSL), National Oceanic and Atmospheric Administration (NOAA), Biogéosciences [UMR 6282] [Dijon] (BGS), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Department of Geography, Hong Kong Baptist University (HKBU), Department of Meteorology [Reading], University of Reading (UOR), This work is part of the I-SITE Bourgogne Franche-Comté Junior Fellowship IMVULA (Grant N°: AAP2-JF-06), and the Alliance Programme 2020 (Grant N°: 608081922), cofunded by the British Council and Campus-France. AC was supported by the NOAA Climate Program Office’s Climate Variability and Predictability (CVP) and Modelling, Analysis, Predictions and Projections (MAPP) programs., and ANR-15-IDEX-0003,BFC,ISITE ' BFC(2015)
Subjects: Environmental sciences, QE1-996.5, 010504 meteorology & atmospheric sciences, [SDU.STU.CL]Sciences of the Universe [physics]/Earth Sciences/Climatology, 13. Climate action, 0207 environmental engineering, Geology, GE1-350, 02 engineering and technology, 020701 environmental engineering, 01 natural sciences, 0105 earth and related environmental sciences
Abstract: 13 pages; International audience; El Niño-Southern Oscillation (ENSO) shows a large diversity of events that is modulated by climate variability and change. The representation of this diversity in climate models limits our ability to predict their impact on ecosystems and human livelihood. Here, we use multiple observational datasets to provide a probabilistic description of historical variations in event location and intensity, and to benchmark models, before examining future system trajectories. We find robust decadal variations in event intensities and locations in century-long observational datasets, which are associated with perturbations in equatorial wind-stress and thermocline depth, as well as extra-tropical anomalies in the North and South Pacific. Some climate models are capable of simulating such decadal variability in ENSO diversity, and the associated large-scale patterns. Projections of ENSO diversity in future climate change scenarios strongly depend on the magnitude of decadal variations, and the ability of climate models to reproduce them realistically over the 21 st century.
Published: 2021

35. An updated checklist and a DNA barcode library for the earthworms (Crassiclitellata, Oligochaeta) of Corsica, France

Author: Marchán, Daniel Fernández, Gérard, Sylvain, Hedde, Mickaël, Rougerie, Rodolphe, Decaëns, Thibaud, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Muséum national d'Histoire naturelle (MNHN), naturalist expedition, 'Our Planet Reviewed – Corsica 2019-2021' organized by the Muséum national d’Histoire naturelle (MNHN, Paris) in collaboration with and funded by the Collectivité de Corse (CdC) and the Office français de la Biodiversité (OFB), and Make Our Planet Great Again Postdoctoral grant from Campus France (mopga-postdoc-3–6111272103)
Subjects: Annelida, Acanthodrilidae, Mediterranean islands, new records, species list, Biodiversity, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, Hormogastridae, endemism, Crassiclitellata, Animalia, Clitellata, DNA barcoding, Animal Science and Zoology, Diporodrilidae, Lumbricidae, Ecology, Evolution, Behavior and Systematics, Taxonomy
Abstract: International audience; Available studies of the earthworm fauna of Corsica reported a total of 36 species, but this regional diversity is probably underestimated considering the relatively modest sampling efforts achieved so far. We conducted a sampling campaign in the context of the program “Our Planet Reviewed”, with the aim to increase sampling coverage and to analyze already known regional diversity with a combination of classical morpho-anatomic characters and DNA barcoding. We sampled a total of 31 locations, and identified 22 previously recorded species and eight new citations belonging to the families Acanthodrilidae Claus, 1880, Hormogastridae Michaelsen, 1900 and Lumbricidae Rafinesque- Schmaltz, 1815. We obtained 525 COI barcodes which clustered into 48 genetic lineages, highlight- ing the importance of molecular techniques for earthworm diversity surveys. Of these lineages, ten are supported by multiple lines of evidence and will be described in a forthcoming taxonomy paper as species new to science. Biogeographical affinities of the identified species showed a lower rate of cosmopolitan species compared to other Mediterranean islands, a certain degree of affinity with Italy and Sardinia, and a high rate of endemism (which could be mirrored by the Balearic Islands and Sardinia). Comparative phylogeographic analyses are needed to understand the history of coloniza- tion and diversification of earthworms on Mediterranean islands, and to investigate the drivers that shaped differences in their diversity.; Les études disponibles sur la faune lombricienne de la Corse font état d’un total de 36 espèces, mais cette diversité régionale est probablement sous-estimée si l’on considère les efforts d’échantillonnage relativement modestes réalisés jusqu’à présent. Nous avons mené une campagne d’échantillonnage dans le cadre du programme « La Planète Revisitée », avec comme objectif d’augmenter la couver- ture d’échantillonnage et d’analyser la diversité régionale en combinant l’utilisation des caractères morpho-anatomiques classiques à celle des codes-barres ADN. Nous avons échantillonné un total de 31 sites, ce qui a permis d’identifier 22 espèces précédemment citées et huit nouvelles signalisa- tions appartenant aux familles Acanthodrilidae Claus, 1880, Hormogastridae Michaelsen, 1900 et Lumbricidae Rafinesque-Schmaltz, 1815. Nous avons obtenu 525 codes-barres ADN qui nous ont permis de délimiter 48 lignées génétiques, soulignant l’importance des techniques moléculaires pour inventorier la diversité des vers de terre. Parmi ces lignées, dix sont soutenues par la morphologie et seront décrites comme espèces nouvelles pour la science dans un article de taxonomie à venir. Les affinités biogéographiques des espèces identifiées montrent un taux relativement faible d’espèces cos- mopolites par rapport à d’autres îles méditerranéennes, un degré d’affinité avec les faunes italiennes et sardes, et un taux élevé d’endémisme (qui pourrait être équivalent à celui des îles Baléares et de la Sardaigne). Des analyses phylogéographiques comparatives pourraient aider à comprendre l’histoire de la colonisation et la diversification des faunes de vers de terre des îles méditerranéennes et à com- prendre les facteurs qui ont façonné les différences dans leur diversité.
Published: 2022

36. Migration collective cellulaire sur des substrats courbes cylindriques

Author: Glentis, Alexandros, Blanch-Mercader, Carles, Balasubramaniam, Lakshmi, Saw, Thuan Beng, d’Alessandro, Joseph, Janel, Sebastien, Douanier, Audrey, Delaval, Benedicte, Lafont, Frank, Lim, Chwee Teck, Delacour, Delphine, Prost, Jacques, Xi, Wang, Ladoux, Benoit, Institut Jacques Monod (IJM (UMR_7592)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Mechanobiology Institute [Singapore] (MBI), National University of Singapore (NUS), CHU Lille, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en Biologie cellulaire de Montpellier (CRBM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Laboratoire Physico-Chimie Curie [Institut Curie] [PCC], Mechanobiology Institute [Singapore] [MBI], Institut Jacques Monod [IJM (UMR_7592)], Centre de recherche en Biologie cellulaire de Montpellier [CRBM], Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL], Cellular Microbiology and Physics of Infection Group [Lille] (CMPI), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, This work was supported by the LABEX 'Who Am I?' (ANR-11-LABX-0071) (to B.L.), the European Research Council (ERC) (grant no. Adv-101019835) (to B.L.), the Marie Skłodowska-Curie Actions, Individual Fellowship, project 846449 (to W.X.), People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007–2013) under REA grant agreement no. PCOFUND-GA-2013-609102, through the PRESTIGE program coordinated by Campus France (to W.X.), funding from the Labex Who Am I? (ANR-11-LABX-0071) and the 'Initiatives d’excellence' (Idex ANR-11-IDEX-0005-02) transverse project BioMechanOE (TP5) (to W.X.), the Agence Nationale de la Recherche (ANR) 'POLCAM' ANR-17-CE13-0013 funded Glentis et al., Sci. Adv. 8, eabn5406 (2022) 14 September 2022 by the French government (to B.L.), the SystemsX EpiPhysX consortium funding (to C.B.-M.), the NR PRC LUCELL grant (ANR-19-CE13-0014-01) (to B.D.), the ANR-10-EQPX-04-01 (to F.L.), the European Regional Development Fund (FEDER-12001407) (to F.L.), the Groupama Foundation—Research Prize for Rare Diseases 2017 (to D.D. and W.X.), the ANR 'LUCELL' ANR-19-CE13-0014 funded by the French government (to B.D. and D.D.), the Fondation pour la Recherche Médicale (FRM) (to D.D.), the Université de Paris IDEX, ANR-18-IDEX-0001, funded by the French government through its 'Investments for the Future' program (to D.D.), the Human Frontier Science Program, RGP0038/2018 (to D.D., C.T.L., T.B.S., J.P., and W.X.), Lee Kuan Yew Postdoctoral fellowship and Tier 1 grant (R-397-000-320-114) from the Ministry of Education (MOE), Singapore (to T.B.S.), and the National Research Foundation, Singapore, under the Mechanobiology Institute at the National University of Singapore (to C.T.L.)., ANR-11-LABX-0071,WHO AM I,Determinants de l'Identité : de la molécule à l'individu(2011), Delacour, Delphine, and Determinants de l'Identité : de la molécule à l'individu - - WHO AM I2011 - ANR-11-LABX-0071 - LABX - VALID
Subjects: [SDV] Life Sciences [q-bio], [PHYS]Physics [physics], Multidisciplinary, [SDV]Life Sciences [q-bio]
Abstract: View the article online https://www.science.org/doi/10.1126/sciadv.abn5406; International audience; Three-dimensional collective epithelial rotation around a given axis represents a coordinated cellular movement driving tissue morphogenesis and transformation. Questions regarding these behaviors and their relationship with substrate curvatures are intimately linked to spontaneous active matter processes and to vital morphogenetic and embryonic processes. Here, using interdisciplinary approaches, we study the dynamics of epithelial layers lining different cylindrical surfaces. We observe large-scale, persistent, and circumferential rotation in both concavely and convexly curved cylindrical tissues. While epithelia of inverse curvature show an orthogonal switch in actomyosin network orientation and opposite apicobasal polarities, their rotational movements emerge and vary similarly within a common curvature window. We further reveal that this persisting rotation requires stable cell-cell adhesion and Rac-1-dependent cell polarity. Using an active polar gel model, we unveil the different relationships of collective cell polarity and actin alignment with curvatures, which lead to coordinated rotational behavior despite the inverted curvature and cytoskeleton order.
Published: 2022

37. Vitamin D induced microbicidal activity against Mycobacterium bovis BCG is dependent on the synergistic activity of bovine peripheral blood cell populations

Author: Susana Flores-Villalva, Aude Remot, Florence Carreras, Nathalie Winter, Stephen V. Gordon, Kieran G. Meade, University College Dublin [Dublin] (UCD), Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias [Mexico] (INIFAP), Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Conway Institute of Biomolecular and Biomedical Research [Dublin, Irlande], Science Foundation Ireland grant to Kieran Meade (17/CDA/4717), Irish Research Council and Campus France via the PHC ‘Ulysses’ Grant Schemes 2020 and 2017, and European Project: 731014,VetBioNet(2017)
Subjects: General Veterinary, Neutrophils, [SDV]Life Sciences [q-bio], Immunology, Mycobacteria, 25(OH)(2)D(3), Vitamin D, Microbicidal activity, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Peripheral blood leukocytes
Abstract: International audience; A growing appreciation is emerging of the beneficial role of vitamin D for health and resistance against infectious diseases, including tuberculosis. However, research has predominantly focused on murine and human species and functional data in bovines is limited. Therefore, the objective of this study was to assess the microbicidal activity and immunoregulatory effect of the vitamin D metabolite 1,25(OH)2D3 on bovine peripheral blood leukocytes (PBL) in response to Mycobacterium bovis BCG (BCG) infection using a combination of functional assays and gene expression profiling. Blood from Holstein-Friesian bull calves with low circulating levels of 25(OH)D was stimulated with 1,25(OH)2D3 for 2 h, and then infected with M. bovis BCG. Results showed that 1,25(OH)2D3 supplementation significantly increased BCG killing by on average 16 %, although responses varied between 1 % and 38 % killing. Serial cell subset depletion was then performed on PBL prior to 1,25(OH)2D3 incubation and BCG infected as before to analyse the contribution of major cell types to mycobacterial growth control. Specific antibodies and either magnetic cell separation or density gradient centrifugation of monocytes, granulocytes, CD3+, CD4+, and CD8+ T lymphocytes were used to capture each cell subset. Results showed that depletion of granulocytes had the greatest impact on BCG growth, leading to a significant enhancement of bacterial colonies. In contrast, depletion of CD4+ or CD8+ T cells individually, or in combination (CD3+), had no impact on mycobacterial growth control. In agreement with our previous data, 1,25(OH)2D3 significantly increased bacterial killing in PBL, in monocyte depleted samples, and a similar trend was observed in the granulocyte depleted subset. In addition, specific analysis of sorted neutrophils treated with 1,25(OH)2D3 showed an enhanced microbicidal activity against both BCG and a virulent strain of M. bovis. Lastly, data showed that 1,25(OH)2D3 stimulation increased reactive oxygen species (ROS) production and the expression of genes encoding host defence peptides (HDP) and pathogen recognition receptors (PRRs), factors that play an important role in the microbicidal activity against mycobacteria. In conclusion, the vitamin D metabolite 1,25(OH)2D3 improves antimycobacterial killing in bovine PBLs via the synergistic activity of monocytes and granulocytes and enhanced activation of innate immunity.
Published: 2022

38. Biotransformation of the fluoroquinolone, levofloxacin, by the white-rot fungus $Coriolopsis\ gallica$

Author: Amal Ben Ayed, Imen Akrout, Quentin Albert, Stéphane Greff, Charlotte Simmler, Jean Armengaud, Mélodie Kielbasa, Annick Turbé-Doan, Delphine Chaduli, David Navarro, Emmanuel Bertrand, Craig B. Faulds, Mohamed Chamkha, Amina Maalej, Héla Zouari-Mechichi, Giuliano Sciara, Tahar Mechichi, Eric Record, Biodiversité et Biotechnologie Fongiques (BBF), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), École Nationale d'Ingénieurs de Sfax | National School of Engineers of Sfax (ENIS), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Service de Pharmacologie et Immunoanalyse (SPI), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de Biotechnologie de Sfax (CBS), and The authors thank PHC-Utique project FUNZYBIO (Campus France Code: 47572QD, CMCU Code: 22G0814) for financial support.
Subjects: Microbiology (medical), levofloxacin, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, laccases, fluoroquinolones, Coriolopsis gallica, biotransformation, dye-decolorizing peroxidase, Plant Science, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Ecology, Evolution, Behavior and Systematics
Abstract: International audience; The wastewater from hospitals, pharmaceutical industries and more generally human and animal dejections leads to environmental releases of antibiotics that cause severe problems for all living organisms. The aim of this study was to investigate the capacity of three fungal strains to biotransform the fluoroquinolone levofloxacin. The degradation processes were analyzed in solid and liquid media. Among the three fungal strains tested, $Coriolopsis\ gallica$ strain CLBE55 (BRFM 3473) showed the highest removal efficiency, with a 15% decrease in antibiogram zone of inhibition for Escherichia coli cultured in solid medium and 25% degradation of the antibiotic in liquid medium based on high-performance liquid chromatography (HPLC). Proteomic analysis suggested that laccases and dye-decolorizing peroxidases such as extracellular enzymes could be involved in levofloxacin degradation, with a putative major role for laccases. Degradation products were proposed based on mass spectrometry analysis, and annotation suggested that the main product of biotransformation of levofloxacin by $Coriolopsis\ gallica$ is an N-oxidized derivative.
Published: 2022

39. Structural Characterization and Rheological and Antioxidant Properties of Novel Polysaccharide from Calcareous Red Seaweed

Author: Faiez Hentati, Latifa Tounsi, Guillaume Pierre, Mohamed Barkallah, Alina Violeta Ursu, Hajer Ben Hlima, Jacques Desbrières, Didier Le Cerf, Imen Fendri, Philippe Michaud, Slim Abdelkafi, Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Université de Lorraine (UL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA), Université de Sfax - University of Sfax, Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU), Laboratoire de Biotechnologie et Amélioration des Plantes (LBAP), Centre de Biotechnologie de Sfax (CBS), Campus France -(Eiffel scholarship program of excellence, and unisian Ministry of Higher Education and Scientific Research
Subjects: Sulfates, sulfated xylogalactan, Jania adhaerens, structure, flow behavior, viscoelasticity, antioxidant activity, Pharmaceutical Science, [CHIM.MATE]Chemical Sciences/Material chemistry, Seaweed, Antioxidants, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, [CHIM.POLY]Chemical Sciences/Polymers, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Polysaccharides, Rhodophyta, Drug Discovery, Salts, Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Abstract: A novel sulfated xylogalactan (JASX) was extracted and purified from the rhodophyceae Jania adhaerens. JASX was characterized by chromatography (GC/MS-EI and SEC/MALLS) and spectroscopy (ATR-FTIR and 1H/13C NMR) techniques. Results showed that JASX was constituted by repeating units of (→3)-β-d-Galp-(1,4)-3,6-α-l-AnGalp-(1→)n and (→3)-β-d-Galp-(1,4)-α-l-Galp-(1→)n substituted on O-2 and O-3 of the α-(1,4)-l-Galp units by methoxy and/or sulfate groups but also on O-6 of the β-(1,3)-d-Galp mainly by β-xylosyl side chains and less by methoxy and/or sulfate groups. The Mw, Mn, Đ, [η] and C* of JASX were respectively 600 and 160 kDa, 3.7, 102 mL.g−1 and 7.0 g.L−1. JASX exhibited pseudoplastic behavior influenced by temperature and monovalent salts and highly correlated to the power-law model and the Arrhenius relationship. JASX presented thixotropic characteristics, a gel-like viscoelastic behavior and a great viscoelasticity character. JASX showed important antioxidant activities, outlining its potential as a natural additive to produce functional foods.
Published: 2022
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40. The Valanginian Weissert Event on the south Tethyan margin: a dynamic paleoceanographic evolution based on the study of calcareous nannofossils

Author: M. Shmeit, F. Giraud, E. Jaillard, S. Reboulet, M. Masrour, J.E. Spangenberg, A. El-Samrani, Institut des Sciences de la Terre (ISTerre), Institut national des sciences de l'Univers (INSU - CNRS)-Institut de recherche pour le développement [IRD] : UR219-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Gustave Eiffel-Université Grenoble Alpes (UGA), Laboratory of Geosciences Georesources and Environment (L2GE), Lebanese University [Beirut] (LU), Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Département de Géologie [Agadir], Université Ibn Zohr [Agadir], Institut des Dynamiques de la Surface Terrestre [Lausanne] (IDYST), Université de Lausanne = University of Lausanne (UNIL), and Financial support by the Ministries offoreign affairs from France and Morocco (PHC project n◦ 031/STU/13),the French IRD, Campus France, and various grants from the ISTerrelaboratory (Grenoble), the Laboratoire de G´eologie de Lyon, theOSUG@2020 Labex, the CNRS SYSTER program, and IODP Francesoutien post-cruise. Additional grants were provided by the Frenchgovernment (bourse BGF), the doctoral school TUE of the Grenoble-Alpes University, and by the TRB team of ISTerre laboratory.
Subjects: [SDU.STU.ST]Sciences of the Universe [physics]/Earth Sciences/Stratigraphy, Paleontology, Biostratigraphy, Surface-water fertility, Oceanography, [SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/Paleontology, Carbon isotope excursion, Central Moroccan margin, Sedimentary evolution
Abstract: 21 pages; International audience; Paleoceanographic conditions across the Valanginian Weissert oceanic anoxic event were reconstructed for the first time on the central Moroccan margin from a quantitative-based calcareous nannofossil study. Two onshore successions in the Essaouira-Agadir Basin and one offshore from DSDP Hole 416A were studied, providing a proximal-distal transect on the central Moroccan margin. The paleoceanographic conditions were reconstructed in an accurate chronostratigraphic framework. The sedimentary evolution of the Essaouira-Agadir Basin shows that sea-level variations controlled the paleogeographic context, clastic input, and nutrient availability. A thick photic zone and mesotrophic conditions favored the development of a diverse nannofossil community before the Weissert Event in the early Valanginian. In all studied successions, a collapse of the nannofossil community was recorded during the onset of the Weissert Event at the early-late Valanginian transition, and was caused by a major sea-level fall. The nannofossil community gradually recovered and reached its highest nannofossil production associated with high surface-water fertility during the Weissert Event in the late Valanginian. This eutrophication is coeval with the high and stable delta C-13(carb) values of the carbon-isotope positive shift. After the Weissert Event, surface-water fertility and nannofossil production decreased but remained higher than during the early Valanginian. Additionally, the nannoconid decline started in the early Valanginian and was linked to a low sea-level. Their recovery in the early late Valanginian occurred during conditions of high sea-level and of high surface-water fertility. The central Moroccan margin is integrated into a global paleoceanographic reconstruction.
Published: 2022

41. Effect of channel density, inverse solutions and connectivity measures on EEG resting-state networks reconstruction: A simulation study

Author: Sahar Allouch, Aya Kabbara, Joan Duprez, Mohamad Khalil, Julien Modolo, Mahmoud Hassan, Laboratoire Traitement du Signal et de l'Image (LTSI), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Azm Center for Research and Biotechnologies, Lebanese University [Beirut] (LU), MINDig, Lebanese Association for Scientific Research [Tripoli] (LASeR), Ecole Doctorale des Sciences et de la Technologie (EDST), Reykjavík University, and The authors would like to acknowledge the Lebanese National Council for Scientific Research (CNRS-L), the Agence Universitaire de la Francophonie (AUF), and the Lebanese University for granting Ms. Allouch a doctoral scholarship. This work was also supported by the Labex Cominlabs project PKSTIM and by the Institute of Clinical Neuroscience of Rennes (Projects EEGCog and EEGNET3). The authors would also like to thank Campus France, Programme Hubert Curien CEDRE (PROJET N° 42257YA), and the Lebanese Association for Scientific Research (LASER) for their support.
Subjects: Functional connectivity, Analytical variability, Channel density, Neurology, Cognitive Neuroscience, EEG resting-state networks, Neural mass model, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Inverse solution
Abstract: International audience; Along with the study of brain activity evoked by external stimuli, the past two decades witnessed an increased interest in characterizing the spontaneous brain activity occurring during resting conditions. The identification of connectivity patterns in this so-called "resting-state" has been the subject of a great number of electrophysiology-based studies, using the Electro/Magneto-Encephalography (EEG/MEG) source connectivity method. However, no consensus has been reached yet regarding a unified (if possible) analysis pipeline, and several involved parameters and methods require cautious tuning. This is particularly challenging when different analytical choices induce significant discrepancies in results and drawn conclusions, thereby hindering the reproducibility of neuroimaging research. Hence, our objective in this study was to shed light on the effect of analytical variability on outcome consistency by evaluating the implications of parameters involved in the EEG source connectivity analysis on the accuracy of resting-state networks (RSNs) reconstruction. We simulated, using neural mass models, EEG data corresponding to two RSNs, namely the default mode network (DMN) and dorsal attentional network (DAN). We investigated the impact of five channel densities (19, 32, 64, 128, 256), three inverse solutions (weighted minimum norm estimate (wMNE), exact low-resolution brain electromagnetic tomography (eLORETA), and linearly constrained minimum variance (LCMV) beamforming) and four functional connectivity measures (phase-locking value (PLV), phase-lag index (PLI), and amplitude envelope correlation (AEC) with and without source leakage correction), on the correspondence between reconstructed and reference networks. We showed that, with different analytical choices related to the number of electrodes, source reconstruction algorithm, and functional connectivity measure, high variability is present in the results. More specifically, our results show that a higher number of EEG channels significantly increased the accuracy of the reconstructed networks. Additionally, our results showed significant variability in the performance of the tested inverse solutions and connectivity measures. Such methodological variability and absence of analysis standardization represent a critical issue for neuroimaging studies that should be prioritized. We believe that this work could be useful for the field of electrophysiology connectomics, by increasing awareness regarding the challenge of variability in methodological approaches and its implications on reported results.
Published: 2023

42. Live-cell micromanipulation of a genomic locus reveals interphase chromatin mechanics

Author: Veer I. P. Keizer, Simon Grosse-Holz, Maxime Woringer, Laura Zambon, Koceila Aizel, Maud Bongaerts, Fanny Delille, Lorena Kolar-Znika, Vittore F. Scolari, Sebastian Hoffmann, Edward J. Banigan, Leonid A. Mirny, Maxime Dahan, Daniele Fachinetti, Antoine Coulon, Dynamique du noyau [Institut Curie], Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Biologie Cellulaire et Cancer, Université Paris sciences et lettres (PSL), Department of Physics [MIT Cambridge], Massachusetts Institute of Technology (MIT), Laboratoire de Physique et d'Etude des Matériaux (UMR 8213) (LPEM), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), LabEx CELL(N)SCALE, LabEx DEEP, ANR, ERC, Institut Curie, ATIP-Avenir (CNRS, INSERM, Plan Cancer), Campus France (PRESTIGE-2018-1-0023), Fondation ARC (PJA 20161204869), NIH (GM114190), MIT-France Seed Fund, Région Île-de-France (Chaire Blaise Pascal), ANR-18-CE12-0023,CHROMAG,Probing genome dynamics and mechanics at the single chromosome level(2018), ANR-11-LABX-0038,CelTisPhyBio,Des cellules aux tissus: au croisement de la Physique et de la Biologie(2011), ANR-11-LABX-0044,DEEP,Développement, Epigénèse, Epigénétique et potentiel de vie(2011), ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010), European Project: 757956,4D-GenEx, European Project: 666003,H2020,H2020-MSCA-COFUND-2014,IC-3i-PhD(2016), Coulon, Antoine, APPEL À PROJETS GÉNÉRIQUE 2018 - Probing genome dynamics and mechanics at the single chromosome level - - CHROMAG2018 - ANR-18-CE12-0023 - AAPG2018 - VALID, Des cellules aux tissus: au croisement de la Physique et de la Biologie - - CelTisPhyBio2011 - ANR-11-LABX-0038 - LABX - VALID, Développement, Epigénèse, Epigénétique et potentiel de vie - - DEEP2011 - ANR-11-LABX-0044 - LABX - VALID, Initiative d'excellence - Paris Sciences et Lettres - - PSL2010 - ANR-10-IDEX-0001 - IDEX - VALID, European Research Council (ERC) Starting Grant - 4D-GenEx - 757956 - INCOMING, and Institut Curie 3-i PhD Program - IC-3i-PhD - - H20202016-09-01 - 2021-09-01 - 666003 - VALID
Subjects: Cell Nucleus, Multidisciplinary, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [PHYS.MECA.BIOM] Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], [SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Genomics, Chromatin, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, Micromanipulation, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Chromosomes, Human, Humans, [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], Interphase
Abstract: International audience; Our understanding of the physical principles organizing the genome in the nucleus is limited by the lack of tools to directly exert and measure forces on interphase chromosomes in vivo and probe their material nature. Here, we introduce an approach to actively manipulate a genomic locus using controlled magnetic forces inside the nucleus of a living human cell. We observed viscoelastic displacements over micrometers within minutes in response to near-piconewton forces, which are consistent with a Rouse polymer model. Our results highlight the fluidity of chromatin, with a moderate contribution of the surrounding material, revealing minor roles for cross-links and topological effects and challenging the view that interphase chromatin is a gel-like material. Our technology opens avenues for future research in areas from chromosome mechanics to genome functions.
Published: 2022

43. Caspase-1-driven neutrophil pyroptosis and its role in host susceptibility to Pseudomonas aeruginosa

Author: Karin Santoni, David Pericat, Leana Gorse, Julien Buyck, Miriam Pinilla, Laure Prouvensier, Salimata Bagayoko, Audrey Hessel, Stephen Adonai Leon-Icaza, Elisabeth Bellard, Serge Mazères, Emilie Doz-Deblauwe, Nathalie Winter, Christophe Paget, Jean-Philippe Girard, Christine T. N. Pham, Céline Cougoule, Renaud Poincloux, Mohamed Lamkanfi, Emma Lefrançais, Etienne Meunier, Rémi Planès, Institut de pharmacologie et de biologie structurale (IPBS), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Pharmacologie des anti-infectieux et antibiorésistance (PHAR2), Université de Poitiers-Institut National de la Santé et de la Recherche Médicale (INSERM), Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Washington University School of Medicine in St. Louis, Washington University in Saint Louis (WUSTL), Universiteit Gent = Ghent University (UGENT), Fonds de Recherche en Santé Respiratoire - Fondation du Souffle, ATIP-Avenir program, FRM 'Amorçage Jeunes Equipes' (AJE20151034460, ERC (StG INFLAME 804249, NIH (AR073752, European Society of Clinical Microbiology and Infectious Diseases (ESCMID, Invivogen-CIFRE PhD grant, Invivogen post-doctoral fellowship, PhD fellowship from the Minister of Research of Mali and Campus France agency., European Project: 804249,INFLAME, Chanteloup, Nathalie Katy, and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)
Subjects: Mice, Knockout, [SDV.IMM] Life Sciences [q-bio]/Immunology, Inflammasomes, Neutrophils, Caspase 1, Interleukin-1beta, Immunology, Exotoxins, Microbiology, Mice, Inbred C57BL, Mice, Virology, Pseudomonas aeruginosa, Pyroptosis, Genetics, Animals, Humans, [SDV.IMM]Life Sciences [q-bio]/Immunology, Parasitology, Apoptosis Regulatory Proteins, Molecular Biology
Abstract: Multiple regulated neutrophil cell death programs contribute to host defense against infections. However, despite expressing all necessary inflammasome components, neutrophils are thought to be generally defective in Caspase-1-dependent pyroptosis. By screening different bacterial species, we found that several Pseudomonas aeruginosa (P. aeruginosa) strains trigger Caspase-1-dependent pyroptosis in human and murine neutrophils. Notably, deletion of Exotoxins U or S in P. aeruginosa enhanced neutrophil death to Caspase-1-dependent pyroptosis, suggesting that these exotoxins interfere with this pathway. Mechanistically, P. aeruginosa Flagellin activates the NLRC4 inflammasome, which supports Caspase-1-driven interleukin (IL)-1β secretion and Gasdermin D (GSDMD)-dependent neutrophil pyroptosis. Furthermore, P. aeruginosa-induced GSDMD activation triggers Calcium-dependent and Peptidyl Arginine Deaminase-4-driven histone citrullination and translocation of neutrophil DNA into the cell cytosol without inducing extracellular Neutrophil Extracellular Traps. Finally, we show that neutrophil Caspase-1 contributes to IL-1β production and susceptibility to pyroptosis-inducing P. aeruginosa strains in vivo. Overall, we demonstrate that neutrophils are not universally resistant for Caspase-1-dependent pyroptosis.
Published: 2022

44. Three families of Asgard archaeal viruses identified in metagenome-assembled genomes

Author: Sofia Medvedeva, Jiarui Sun, Natalya Yutin, Eugene V. Koonin, Takuro Nunoura, Christian Rinke, Mart Krupovic, Virologie des archées - Archaeal Virology, Université Paris Cité (UPCité)-Microbiologie Intégrative et Moléculaire (UMR6047), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Skolkovo Institute of Science and Technology [Moscow] (Skoltech), University of Queensland [Brisbane], National Institutes of Health [Bethesda] (NIH), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), The work in the M.K. laboratory is supported by grants from l’Agence Nationale de la Recherche (nos. ANR-20-CE20-0009-02 and ANR-21-CE11-0001-01) and Ville de Paris (Emergence(s) project MEMREMA). S.M. was supported by the Metchnikov fellowship from Campus France and Russian Science Foundation (grant no. 19-74-20130). N.Y. and E.V.K. are supported by the Intramural Research Program of the National Institutes of Health of the USA (National Library of Medicine). The work of C.R. and J.S. is funded by the Australian Research Council Future Fellow Award (no. FT170100213, to CR). T.N. was partly supported by MEXT KAKENHI (grant nos. JP19H05684 within JP19H05679 (Post-Koch Ecology) and 16H06429, 16K21723 and 16H06437 (NeoVirology))., ANR-20-CE20-0009,VIROMET,Devoiler le virome des archées methanogenes(2020), and ANR-21-CE11-0001,ArcFus,Protéines de classe II de fusion membranaire chez les archées(2021)
Subjects: Microbiology (medical), Immunology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Genetics, Cell Biology, Applied Microbiology and Biotechnology, Microbiology
Abstract: International audience; Asgardarchaeota harbour many eukaryotic signature proteins and are widely considered to represent the closest archaeal relatives of eukaryotes. Whether similarities between Asgard archaea and eukaryotes extend to their viromes remains unknown. Here we present 20 metagenome-assembled genomes of Asgardarchaeota from deep-sea sediments of the basin off the Shimokita Peninsula, Japan. By combining a CRISPR spacer search of metagenomic sequences with phylogenomic analysis, we identify three family-level groups of viruses associated with Asgard archaea. The first group, verdandiviruses, includes tailed viruses of the class Caudoviricetes (realm Duplodnaviria); the second, skuldviruses, consists of viruses with predicted icosahedral capsids of the realm Varidnaviria; and the third group, wyrdviruses, is related to spindle-shaped viruses previously identified in other archaea. More than 90% of the proteins encoded by these viruses of Asgard archaea show no sequence similarity to proteins encoded by other known viruses. Nevertheless, all three proposed families consist of viruses typical of prokaryotes, providing no indication of specific evolutionary relationships between viruses infecting Asgard archaea and eukaryotes. Verdandiviruses and skuldviruses are likely to be lytic, whereas wyrdviruses potentially establish chronic infection and are released without host cell lysis. All three groups of viruses are predicted to play important roles in controlling Asgard archaea populations in deep-sea ecosystems.
Published: 2022

45. Mycobacterial infection of precision cut lung slices reveals type 1 interferon pathway is locally induced by Mycobacterium bovis but not M. tuberculosis

Author: Remot, Aude, Carreras, Florence, Doz-Deblauwe, Emilie, Boschiroli, Maria-Laura, Browne, John, Gordon W, Stephen, Winter, Nathalie, Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Zoonoses Bactériennes (UZB), Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), University College Dublin [Dublin] (UCD), This work was supported by the Veterinary Biocontained research facility Network (VETBIONET), the ANR EpiLungCell (grant ANR-17-CE20-0018) and FEDER/Region Centre Val de Loire ANIMALT grant (FEDER convention number EX007516, Region Centre convention number 2019-00134936, research program number AE-2019-1850). Mobilities between France and Irland were supported by the 'ONE-TB' project (PHC Ulysses, funded by Campus France and the Irish Research Council), and the Fédération de Recherche en Infectiologie du Centre Val de Loire (FéRI)., ANR-17-CE20-0018,EpiLungCell,Etablissement de lignées cellulaires de ruminants pour la réplication et l'étude de pathogènes(2017), and European Project: 731014,VetBioNet(2017)
Subjects: cattle, [SDV]Life Sciences [q-bio], ex vivo, [SDV.IMM]Life Sciences [q-bio]/Immunology, One Health, innate immunity, Mycobacterium bovis
Abstract: International audience; Objective: Mycobacterium bovis (Mb) responsible for bovine TB, and Mycobacterium tuberculosis (Mtb) for human TB, are greater than 99.9% identical at the genetic level. It has been shown that the human pathogen, Mtb, shows reduced ability to cause disease in cattle (PMID: 20049086); furthermore, epidemiological studies have shown that Mb is less able to cause disease in humans (PMID: 5297551). However, the genetic reason(s) why one is an animal pathogen and the other human pathogen is not known. If we were able to determine the basis for the ability of these pathogens to cause disease, we would be able to develop better control tools, such as vaccines and diagnostics, to control these diseases in both humans and animals. Mb causes a chronic infection preferentially targeting the lung. The early steps after infection that influence the physiopathology of the disease are still poorly understood. We aim to get a deeper understanding of the physiopathology of bTB in the target species i.e. bovine and in the target organ, the lung. We compared the host tissue responses after Mb and Mtb infection. Such comparative study of human and animal pathogens is a key example of the One Health concept that explores the interrelation of human and animal health.Key findings: We set up a protocol for ex vivo infection of bovine lungs (Precision-cut lung slices; PCLS) that helps deciphering early steps following infection in the natural environment.We performed in situ imaging of PCLS infected with a fluorescent Mb strain, and localized our bacilli in the alveolar compartment, in contact with pneumocytes, near or inside alveolar macrophages. We analyzed the global tissue signature following infection by two Mb strains (AF2122 and Mb3601) and two Mtb strains (H37Rv and BTB1558). We performed a transcriptomic analysis using two different technologies, NanoString nCounter Analysis System and Fluidigm Biomark; and measured cytokines/chemokines in culture supernatant using a bovine 15-plex assay. We measured induction of inflammatory genes and chemokines for granulocyte recruitment. Tissue responses were higher at day 2 than day 1 post infection, and different between two breeds. Strikingly, the type I interferon pathway was only induced by Mb but not Mtb strains indicating that this pathway may be involved in mycobacterial virulence and host tropism. Impact: We aim to improve our comprehension of host-pathogen interactions ex vivo while reducing reliance on animal experiments, and contribute to future disease control strategies through a One Health approach.
Published: 2022

46. Enhancing Cycling Stability and Specific Capacitance of Vanadium Nitride Electrodes by Tuning Electrolyte Composition

Author: Emile Haye, Yuanyuan Miao, David Pilloud, Camille Douard, Rabah Boukherroub, Jean-François Pierson, Thierry Brousse, Stéphane Lucas, Laurent Houssiau, Jean-Jacques Pireaux, Amine Achour, Namur Institute of Structured Matter (NISM), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Institut Jean Lamour (IJL), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut des Matériaux Jean Rouxel (IMN), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Nantes université - UFR des Sciences et des Techniques (Nantes univ - UFR ST), Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - Ecole Polytechnique de l'Université de Nantes (Nantes Univ - EPUN), Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Réseau sur le stockage électrochimique de l'énergie (RS2E), Aix Marseille Université (AMU)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Collège de France (CdF (institution))-Université de Picardie Jules Verne (UPJV)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Université Grenoble Alpes (UGA)-Nantes Université (Nantes Univ)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Université de Montpellier (UM), Université de Namur [Namur] (UNamur), Laboratoire Interdisciplinaire de Spectroscopie Electronique (LISE), Facultés Universitaires Notre Dame de la Paix (FUNDP), The authors thank the Synthesis, Irradiation & Analysis of Materials (SIAM), Morph’IM and PC² platforms of the University of Namur for XPS, SEM and XRD measurements.E.H., S.L. D.P. and J.F.P. acknowledge Wallonia Brussel International and Campus France for funding the PLASMOCAPS project, done is the framework of Belgo-French Project HUBERT CURIEN TOURNESOL. TB and CD acknowledge LABEX STOREEX (ANR 10-LABX-00, and ANR-10-LABX-0076,STORE-EX,Laboratory of excellency for electrochemical energy storage(2010)
Subjects: [SPI]Engineering Sciences [physics], Renewable Energy, Sustainability and the Environment, Thin film electrode, Materials Chemistry, Electrochemistry, Pseudocapacitance, Condensed Matter Physics, Vanadium nitride, Cycling stability, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials
Abstract: In this study, the tuning of the KOH electrolyte composition is proposed as a strategy to drastically limit the capacitance fade of vanadium nitride (VN) electrode. We demonstrate that the use of a V5+ (as VO4 3− vanadates anions) containing KOH electrolyte enhances the cycling stability of VN thin film electrode: a loss of 59% of the capacitance is observed for the electrode tested in KOH over 3000 consecutive cycles. After V5+ addition in the electrolyte, the capacitance fade is decreased to 23%. Furthermore, the presence of V5+ species in the solution leads to VN capacitance enhancement from 379 mF cm−2 for V5+ ions free electrolyte up to 577 mF cm−2 at 5 mV s−1 for V5+-containing KOH solution. The enhanced cycling stability is attributed to the stabilization of an oxide/oxynitride layer at the VN surface, instead of its dissolution, thanks to the chemical equilibrium shift of the VN dissolution reaction. This simple and innovative strategy consisting in tuning the electrolyte composition opens new pathways for other systems that suffer from electrode dissolution in the electrolyte while being electrochemically cycled.
Published: 2022

47. Microwave absorption by carbon-based materials and structures

Author: Polina Kuzhir, Alain Celzard, Xiaobo Chen, University of Eastern Finland, Institut Jean Lamour (IJL), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), University of Missouri [Kansas City] (UMKC), University of Missouri System, Academy of Finland via Flagship Program Photonics Research and Innovation (PREIN), Decision No. 32016, Campus France and Lithuanian Science Council through the joint program PHC Gilibert No. 46414VC 'Hybrid gels for electromagnetic applications,', NATO Science for Peace and Security Program [Grant No. G5697 CERTAIN, 'Globular carbon-based structures and metamaterials for enhanced electromagnetic protection']., European Project: 823728 ,DiSeTCom, and European Project: 823878,TERASSE
Subjects: [PHYS]Physics [physics], Microwave absorptions, microwave, nanocarbon, graphene, Carbohydrates, General Physics and Astronomy, Composite materials, 3D printing, metasurface, Carbon based materials, Chemical engineering, Electromagnetic spectrum, Electromagnetic compatibility, carbon nanotube, absorption, Mechanosynthesis
Abstract: 'Microwave absorption by carbon-based materials and structures' special topic Editorial&nbsp
Published: 2022

48. Single‐Step Synthesis of Exfoliated Ti3C2TxMXene through NaBF4/HCl Etching as Electrode Material for Asymmetric Supercapacitor

Author: Ghosh, Meena, Szunerits, Sabine, Cao, Ning, Kurungot, Sreekumar, Boukherroub, Rabah, CSIR-National Chemical Laboratory, Council of Scientific and Industrial Research (CSIR), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), China University of Petroleum, and M.G. would like to thank Centre Franco-Indien pour la Promotion de la Recherche Avancée (CEFIPRA) and Campus France for supporting the research stay in France through Raman-Charpak Fellowship program. R.B. and S.S. thank the financial support from the Centre National de la Recherche Scientifique (CNRS), University of Lille, and Hauts-de-France region. M.G. and K.S. acknowledge CSIR, India for the research fellowship and Department of Science and Technology (DST), New Delhi, for the research funding (GAP328726, Sanction Order No CRG/2019/000277), respectively.
Subjects: [SPI]Engineering Sciences [physics]
Abstract: International audience; Two-dimensional Ti3C2Tx MXene, derived from the parent Ti3AlC2 phase, is considered a promising electrode material for electrochemical energy storage applications. Ti3C2Tx MXene synthesis methods often employ concentrated hydrofluoric acid (HF), which is harsh, corrosive, and hazardous to the environment. Herein, we report the preparation of Ti3C2Tx MXene following a new synthetic route under a mild reaction condition comprising an aqueous solution of NaBF4 and HCl. This etching environment at a moderate temperature of 130 °C effectively removes the Al layer of the Ti3AlC2 precursor, assisted by the in situ formed HF in the reaction medium. Electron microscopy images of the as-prepared Ti3C2Tx (MX-130) reveal a partially exfoliated nanosheet-like morphology. The material displays a specific capacitance of 262 F g−1 (three-electrode assembly, 1 A g−1, −0.85 to −0.25 V vs. Hg/Hg2SO4) in 1 M H2SO4 electrolyte. The achieved specific capacitance is superior to that of Ti3C2Tx prepared via the common HF-treatment (25 F g−1). Additionally, the potential application of the optimized MXene as a negative electrode material is demonstrated in a quasi-solid-state RuO2|MX-130 asymmetric supercapacitor device.
Published: 2022

49. Conjuring up a ghost: structural and functional characterization of FhuF, a ferric siderophore reductase from E. coli

Author: Estelle Lebègue, T Cordeiro, Frédéric Barrière, Inês B. Trindade, G Hernandez, Mario Piccioli, Ricardo O. Louro, Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Firenze = University of Florence (UniFI), 810856, H2020 Spreading Excellence and Widening Participation, CA15133, European Cooperation in Science and Technology, 40814ZE, Campus France, PD/BD/135187/2017, FCT– Fundação para a Ciência e a Tecnologia, I.P., Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), and Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI)
Subjects: 0301 basic medicine, Models, Molecular, Siderophore, Subfamily, FMN Reductase, Stereochemistry, Redox-Bohr effect, Reductase, Ferric-siderophore reductase, Biochemistry, 2Fe–2S protein, Inorganic Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Protein Domains, medicine, Escherichia coli, [CHIM]Chemical Sciences, Cysteine, Ferredoxin, Ferrichrome, Original Paper, 030102 biochemistry & molecular biology, Chemistry, Nuclear magnetic resonance spectroscopy, Iron uptake, Small molecule, 030104 developmental biology, Ferric, Oxidation-Reduction, medicine.drug
Abstract: Graphic abstract Iron is a fundamental element for virtually all forms of life. Despite its abundance, its bioavailability is limited, and thus, microbes developed siderophores, small molecules, which are synthesized inside the cell and then released outside for iron scavenging. Once inside the cell, iron removal does not occur spontaneously, instead this process is mediated by siderophore-interacting proteins (SIP) and/or by ferric-siderophore reductases (FSR). In the past two decades, representatives of the SIP subfamily have been structurally and biochemically characterized; however, the same was not achieved for the FSR subfamily. Here, we initiate the structural and functional characterization of FhuF, the first and only FSR ever isolated. FhuF is a globular monomeric protein mainly composed by α-helices sheltering internal cavities in a fold resembling the “palm” domain found in siderophore biosynthetic enzymes. Paramagnetic NMR spectroscopy revealed that the core of the cluster has electronic properties in line with those of previously characterized 2Fe–2S ferredoxins and differences appear to be confined to the coordination of Fe(III) in the reduced protein. In particular, the two cysteines coordinating this iron appear to have substantially different bond strengths. In similarity with the proteins from the SIP subfamily, FhuF binds both the iron-loaded and the apo forms of ferrichrome in the micromolar range and cyclic voltammetry reveals the presence of redox-Bohr effect, which broadens the range of ferric-siderophore substrates that can be thermodynamically accessible for reduction. This study suggests that despite the structural differences between FSR and SIP proteins, mechanistic similarities exist between the two classes of proteins. Supplementary Information The online version contains supplementary material available at 10.1007/s00775-021-01854-y.
Published: 2021

50. Achieving high circularly polarized luminescence with push–pull helicenic systems: from rationalized design to top-emission CP-OLED applications

Author: Bassem Jamoussi, Laura Abella, Benoit Racine, Sylvia Meunier-Della-Gatta, Thierry Roisnel, Nicolas Vanthuyne, Etienne Quesnel, Ludovic Favereau, Grégory Pieters, Kais Dhbaibi, Jeanne Crassous, Jochen Autschbach, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Synthèse Caractérisation Analyse de la Matière (ScanMAT), Université de Rennes (UR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), University at Buffalo [SUNY] (SUNY Buffalo), State University of New York (SUNY), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut des Sciences Moléculaires de Marseille (ISM2), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), King Abdulaziz University, Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), National Science Foundation, NSF: CHE-1855470, University at Buffalo, UB, University of Gabès, UNIVGB, Campus France, Université de Rennes 1, Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut de Chimie du CNRS (INC)
Subjects: Dipole transition moment, Luminescence, Exciton, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, Crucial parameters, Stereochemistry, Theoretical investigations, OLED, [CHIM]Chemical Sciences, Relative orientation, Circular polarization, Luminescence process, business.industry, Excited states, General Chemistry, 021001 nanoscience & nanotechnology, Molecular orientation, 0104 chemical sciences, Organic light emitting diodes (OLED), Chemistry, Chiroptical properties, Dipole, Circularly polarized luminescence, Helicene, chemistry, Excited state, Optoelectronics, Mutual orientation, 0210 nano-technology, business, Magnetic dipole
Abstract: While the development of chiral molecules displaying circularly polarized luminescence (CPL) has received considerable attention, the corresponding CPL intensity, glum, hardly exceeds 10−2 at the molecular level owing to the difficulty in optimizing the key parameters governing such a luminescence process. To address this challenge, we report here the synthesis and chiroptical properties of a new family of π-helical push–pull systems based on carbo[6]helicene, where the latter acts as either a chiral electron acceptor or a donor unit. This comprehensive experimental and theoretical investigation shows that the magnitude and relative orientation of the electric (μe) and magnetic (μm) dipole transition moments can be tuned efficiently with regard to the molecular chiroptical properties, which results in high glum values, i.e. up to 3–4 × 10−2. Our investigations revealed that the optimized mutual orientation of the electric and magnetic dipoles in the excited state is a crucial parameter to achieve intense helicene-mediated exciton coupling, which is a major contributor to the obtained strong CPL. Finally, top-emission CP-OLEDs were fabricated through vapor deposition, which afforded a promising gEl of around 8 × 10−3. These results bring about further molecular design guidelines to reach high CPL intensity and offer new insights into the development of innovative CP-OLED architectures., A CPL intensity of up to 3 × 10−2 is achieved in π-extended 6-helicene derivatives, owing to an intense helicene-mediated exciton coupling. Corresponding top-emission CP-OLEDs afforded a promising gEl of around 8 × 10−3.
Published: 2021

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