1. Onset of Spinal Cord Astrocyte Precursor Emigration from the Ventricular Zone Involves the Zeb1 Transcription Factor
- Author
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Douglas S. Darling, David Ohayon, C. Claus Stolt, Tsuyoshi Takagi, Alexandre Pattyn, Alain Garces, William D. Richardson, Yujiro Higashi, Chadi Soukkarieh, Willy Joly, Eric Agius, Jean-Charles Sabourin, Patrick Carroll, Jean-Philippe Hugnot, Pascal de Santa Barbara, Institut des Neurosciences de Montpellier - Déficits sensoriels et moteurs (INM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), CHU Toulouse [Toulouse], Faculté des Sciences [Damascus], Damascus University, Centre du service humain Aichi, Physiopathologie et thérapie des déficits sensoriels et moteurs, Université Montpellier 2 - Sciences et Techniques (UM2)-IFR76-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de biologie du développement (CBD), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), University of Louisville, Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université Erlangen-Nürnberg, Université College London, Saarland University [Saarbrücken], Développement et évolution du système nerveux (DESN), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), MORNET, Dominique, Institut des Neurosciences de Montpellier (INM), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Université de Damas = Damascus University, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), and École normale supérieure - Paris (ENS-PSL)
- Subjects
0301 basic medicine ,Aging ,cell migration ,[SDV]Life Sciences [q-bio] ,Regulator ,Biology ,General Biochemistry, Genetics and Molecular Biology ,White matter ,Mice ,03 medical and health sciences ,astrocyte ,Cell Movement ,medicine ,Animals ,Zeb2 ,Progenitor cell ,lcsh:QH301-705.5 ,development ,ComputingMilieux_MISCELLANEOUS ,Body Patterning ,Zeb1 ,GFAP ,Stem Cells ,Gene Expression Regulation, Developmental ,Zinc Finger E-box-Binding Homeobox 1 ,spinal cord ,Cell Differentiation ,Embryo, Mammalian ,Spinal cord ,Cell biology ,Neuroepithelial cell ,[SDV] Life Sciences [q-bio] ,Cadherin-1 ,030104 developmental biology ,medicine.anatomical_structure ,lcsh:Biology (General) ,Astrocytes ,Mutation ,Immunology ,Ventricular zone ,oligodendrocyte ,Astrocyte ,Sox9 - Abstract
SummaryDuring spinal cord development, astrocyte precursors arise from neuroepithelial progenitors, delaminate from the ventricular zone, and migrate toward their final locations where they differentiate. Although the mechanisms underlying their early specification and late differentiation are being deciphered, less is known about the temporal control of their migration. Here, we show that the epithelial-mesenchymal transition regulator Zeb1 is expressed in glial precursors and report that loss of Zeb1 function specifically delays the onset of astrocyte precursor delamination from the ventricular zone, correlating with transient deregulation of the adhesion protein Cadherin-1. Consequently, astrocyte precursor invasion into the Zeb1−/− mutant white matter is delayed, and induction of their differentiation is postponed. These findings illustrate how fine regulation of adhesive properties influences the onset of neural precursor migration and further support the notion that duration of exposure of migrating astrocyte precursors to environmental cues and/or their correct positioning influence the timing of their differentiation.
- Published
- 2016