1. Angiogenin in the Neurogenic Subventricular Zone After Stroke
- Author
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Gabriel-Salazar, Marina, Lei, Ting, Grayston, Alba, Costa, Carme, Medina-Gutiérrez, Esperanza, Comabella, Manuel, Montaner, Joan, Rosell Novel, Anna, Universitat Autònoma de Barcelona, Institut Català de la Salut, [Gabriel-Salazar M, Lei T, Grayston A, Medina-Gutiérrez E, Montaner J, Rosell A] Laboratori de Recerca Neurovascular, Servei de Neurologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Costa C, Comabella M] Servei de Neurologia-Neuroimmunologia, Centre d'Esclerosi Múltiple de Catalunya (CEMCAT), Barcelona, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain, and Vall d'Hebron Barcelona Hospital Campus
- Subjects
0301 basic medicine ,Cèl·lules mare neurals ,Angiogenin ,Neurogenesis ,animal diseases ,Malalties cerebrovasculars - Tractament ,Subventricular zone ,enfermedades del sistema nervioso::enfermedades del sistema nervioso central::enfermedades cerebrales::trastornos cerebrovasculares::accidente cerebrovascular [ENFERMEDADES] ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Neuroblast ,Neuroblast migration ,Neurosphere ,Neurorepair ,medicine ,Neural stem/progenitor cells ,neural stem/progenitor cells ,RC346-429 ,Exercise ,Otros calificadores::/terapia [Otros calificadores] ,neurorepair ,Original Research ,exercise ,células::células madre::células madre nerviosas [ANATOMÍA] ,Other subheadings::/therapy [Other subheadings] ,Nestin ,Cells::Stem Cells::Neural Stem Cells [ANATOMY] ,stroke ,Neural stem cell ,Cell biology ,Stroke ,Nervous System Diseases::Central Nervous System Diseases::Brain Diseases::Cerebrovascular Disorders::Stroke [DISEASES] ,neurogenesis ,030104 developmental biology ,medicine.anatomical_structure ,nervous system ,Neurology ,Neurology. Diseases of the nervous system ,Neurology (clinical) ,angiogenin ,030217 neurology & neurosurgery - Abstract
Angiogenina; Exercici; Neuroreparació Angiogenina; Ejercicio; Neuroreparación Angiogenin; Exercise; Neurorepair Ischemic stroke is a leading cause of death and disability worldwide with effective acute thrombolytic treatments. However, brain repair mechanisms related to spontaneous or rehabilitation-induced recovery are still under investigation, and little is known about the molecules involved. The present study examines the potential role of angiogenin (ANG), a known regulator of cell function and metabolism linked to neurological disorders, focusing in the neurogenic subventricular zone (SVZ). Angiogenin expression was examined in the mouse SVZ and in SVZ-derived neural stem cells (NSCs), which were exposed to exogenous ANG treatment during neurosphere formation as well as in other neuron-like cells (SH-SY5Y). Additionally, male C57Bl/6 mice underwent a distal permanent occlusion of the middle cerebral artery to study endogenous and exercise-induced expression of SVZ-ANG and neuroblast migration. Our results show that SVZ areas are rich in ANG, primarily expressed in DCX+ neuroblasts but not in nestin+NSCs. In vitro, treatment with ANG increased the number of SVZ-derived NSCs forming neurospheres but could not modify SH-SY5Y neurite differentiation. Finally, physical exercise rapidly increased the amount of endogenous ANG in the ipsilateral SVZ niche after ischemia, where DCX-migrating cells increased as part of the post-stroke neurogenesis process. Our findings position for the first time ANG in the SVZ during post-stroke recovery, which could be linked to neurogenesis. MG-S was supported by a pre-doctoral fellowship from the Vall d'Hebron Research Institute, AR was supported by the Miguel Servet program and AG holds a pre-doctoral fellowship both from Instituto Carlos III (CPII15/00003 and FI17/00073, respectively), and TL was supported by the China Scholarship Council (CSC-201706170048). This research has been funded by grants PI16/00981, PI19/00186, and RD16/0019/0021 (RETICS-INVICTUS PLUS) from the Instituto de Salud Carlos III co-financed by the European Regional Development Funds, and by the Generalitat de Catalunya (2017SGR 1427).
- Published
- 2021