1. Expression profiling of Wnt signaling genes during gonadal differentiation and gametogenesis in rainbow trout
- Author
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Barbara Nicol, Yann Guiguen, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), SCRIBE Campus de Beaulieu, Institut National de la Recherche Agronomique (INRA), Institut Fédératif de Recherche - Génétique Fonctionnelle Agronomie et Santé (IFR 140 GFAS), Plateforme Génomique Santé Biogenouest®, Brittany province (Region Bretagne), INRA Institute (PHASE department), European Community [222719-LIFECYCLE], Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Brittany province (Région Bretagne) - INRA Institute (PHASE department), and European Project: 222719,EC:FP7:KBBE,FP7-KBBE-2007-2A,LIFECYCLE(2009)
- Subjects
Male ,Embryology ,medicine.medical_specialty ,endocrine system ,gamétogenèse ,Sex Differentiation ,Endocrinology, Diabetes and Metabolism ,[SDV]Life Sciences [q-bio] ,Ovary ,Biology ,Real-Time Polymerase Chain Reaction ,Oogenesis ,Gametogenesis ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Testis ,medicine ,Animals ,RSPO1 ,Wnt Signaling Pathway ,In Situ Hybridization ,030304 developmental biology ,teleost fish ,0303 health sciences ,Sexual differentiation ,urogenital system ,Wnt signaling pathway ,rainbow trout ,Wnt signaling ,GENOMIQUE ,Cell biology ,Gene expression profiling ,Endocrinology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Oncorhynchus mykiss ,Female ,Development of the gonads ,Developmental Biology ,SALMONIDE - Abstract
Wnt signaling plays major roles in various processes, including ovarian differentiation and development in mammals. In order to explore its potential implication during gonadal development in a nonmammalian vertebrate species, expression of Wnt signaling genes was investigated in rainbow trout during gonadal differentiation and gametogenesis. Multiple Wnt pathway genes were expressed and exhibited distinct expression patterns. In ovary, tcf7 was highly expressed during early differentiation, whereas no sexually dimorphic expression of rspo1 was detected. During later ovarian development, wnt11 was highly expressed in granulosa cells and oocytes suggesting an implication in folliculogenesis and oogenesis, whereas wnt9b was principally detected in granulosa cells. In testis, Wnt pathway genes were mostly expressed during early spermatogenesis. Overall, these present results suggest that Wnt signaling is implicated in multiple processes of male and female gonadal development and provide basis for future studies on Wnt signaling functions in teleost fish gonads.
- Published
- 2011