133 results on '"Jirawat Yongsawatdigul"'
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2. Effects of collagen, chitosan and mixture on fibroblast responses and angiogenic activities in <scp>2D</scp> and <scp>3D</scp> in vitro models
- Author
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Thunwa Binlateh, Pilaiwanwadee Hutamekalin, Jirawat Yongsawatdigul, Montarop Yamabhai, and Paiboon Jitprasertwong
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Biomaterials ,Metals and Alloys ,Biomedical Engineering ,Ceramics and Composites - Published
- 2023
3. Prolyl oligopeptidase inhibition and cellular antioxidant activities of a corn gluten meal hydrolysate
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Phiromya Chanajon, Parinya Noisa, and Jirawat Yongsawatdigul
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Organic Chemistry ,Food Science - Published
- 2022
4. Isolation, identification, and in vivo evaluation of the novel antihypertensive peptide, VSKRLNGDA, derived from chicken blood cells
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Wasana Wongngam, Sittiruk Roytrakul, Takakazu Mitani, Shigeru Katayama, Soichiro Nakamura, and Jirawat Yongsawatdigul
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Bioengineering ,Applied Microbiology and Biotechnology ,Biochemistry - Published
- 2022
5. Development of Flavor and Taste Components of Sous-Vide-Cooked Nile Tilapia (
- Author
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Jaksuma, Pongsetkul, Jirawat, Yongsawatdigul, Surintorn, Boonanuntanasarn, and Soottawat, Benjakul
- Abstract
This study aims to shed light on the association between non-volatile and volatile compounds related to flavor/taste characteristics as well as sensory acceptability of Nile tilapia fillet (
- Published
- 2022
6. Extremely halophilic strains of Halobacterium salinarum as a potential starter culture for fish sauce fermentation
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Natteewan Udomsil, Sirinya Pongjanla, Sureelak Rodtong, Somboon Tanasupawat, and Jirawat Yongsawatdigul
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Food Science - Abstract
Proteinase-producing halophilic archaea were isolated from Thai fish sauce collected from industrial fermentation tanks at various periods of fermentation. Five isolates namely, J-1-S4, J-1-S13, J-1-S22, 2 m-40-15-R2, and P-1-S8, were identified as Halobacterium salinarum with slightly different colony and morphological characteristics among isolates. Starters of five isolates were prepared and added to the anchovy mixed with 25% solar salt and fermented for 180 days at 30-35°C. Halophilic bacteria/archaea counts of inoculated samples were decreased and undetected after 120 days of fermentation. At 180 days of fermentation, α-amino group contents of inoculated fish sauce samples (856-1010 mM) and total nitrogen content (2.2%-2.5%) were higher than the control without archaea inoculation (p 0.05). All samples contained low amounts of biogenic amines, suggesting that all starters were not biogenic amine formers. The major volatile compound found in samples inoculated with H. salinarum P-1-S8 and H. salinarum 2 m-40-15-R2 was 3-methylbutanal, which contributes to the meaty note. Dimethyl disulfide, a compound that contributes to fecal note, was detected in all inoculated samples in a lower amount than in the commercial fish sauce (p 0.05). Thus, H. salinarum accelerated protein hydrolysis and produced desirable volatile compounds during fish sauce fermentation in a strain-specific manner.
- Published
- 2022
7. Isolation, identification, and mode of action of antibacterial peptides derived from egg yolk hydrolysate
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Thippawan Pimchan, Fu Tian, Kanjana Thumanu, Sureelak Rodtong, and Jirawat Yongsawatdigul
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Animal Science and Zoology ,General Medicine - Published
- 2023
8. Thigh muscle metabolic response is linked to feed efficiency and meat characteristics in slow-growing chicken
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Pramin Kaewsatuan, Chotima Poompramun, Satoshi Kubota, Jirawat Yongsawatdigul, Wittawat Molee, Pekka Uimari, and Amonrat Molee
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Animal Science and Zoology ,General Medicine - Published
- 2023
9. Ultrasound-assisted extraction of collagen from broiler chicken trachea and its biochemical characterization
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Kitsanapong Kaewbangkerd, Ali Hamzeh, and Jirawat Yongsawatdigul
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Inorganic Chemistry ,Acoustics and Ultrasonics ,Organic Chemistry ,Chemical Engineering (miscellaneous) ,Environmental Chemistry ,Radiology, Nuclear Medicine and imaging - Published
- 2023
10. Corn gluten meal peptides inhibit prolyl oligopeptidase and modulate α-synuclein aggregation in KCl-treated SH-SY5Y cells
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Phiromya Chanajon, Fu Tian, Parinya Noisa, Sittiruk Roytrakul, and Jirawat Yongsawatdigul
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Nutrition and Dietetics ,Medicine (miscellaneous) ,Food Science - Published
- 2023
11. Physicochemical and Antioxidant Properties of Fish Sauce Prepared by Virgibacillus sp. Starter Cultures Addition and Reduced Salt Process
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Chompoonuch Khongla, Jirawat Yongsawatdigul, Nawaporn Lapsongphon, and Sureelak Rodtong
- Subjects
chemistry.chemical_classification ,Antioxidant ,Starter ,chemistry ,Virgibacillus sp ,medicine.medical_treatment ,medicine ,Salt (chemistry) ,%22">Fish ,Food science ,Aquatic Science ,Food Science - Abstract
Antioxidant activity and peptide profiles of fish sauce samples prepared under varied conditions, namely traditional process, starter culture addition, and a reduced salt process, were determined. ...
- Published
- 2021
12. Gelation and vibrational spectroscopy of tropical surimi induced by ascorbic acid and hydrogen peroxide
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Kanjana Thumanu, Jirawat Yongsawatdigul, and Danou Pao
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Fish Proteins ,Food Handling ,030309 nutrition & dietetics ,Infrared spectroscopy ,Ascorbic Acid ,Vibration ,Hydrophobic effect ,03 medical and health sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Fish Products ,Spectroscopy, Fourier Transform Infrared ,Animals ,Colloids ,Fourier transform infrared spectroscopy ,Hydrogen peroxide ,Mechanical Phenomena ,0303 health sciences ,Myosin Heavy Chains ,biology ,Hydrogen Peroxide ,04 agricultural and veterinary sciences ,Ascorbic acid ,biology.organism_classification ,040401 food science ,Sea Bream ,Breaking force ,chemistry ,Polymerization ,Threadfin bream ,Gels ,Hydrophobic and Hydrophilic Interactions ,Food Science ,Nuclear chemistry - Abstract
The combined effect of ascorbic acid (AsA) and hydrogen peroxide (H2 O2 ) on gel-forming ability and structural changes of lizardfish (LZ) and threadfin bream (TB) surimi were investigated. Addition of 0.15% AsA and 0.1% H2 O2 greatly increased breaking force and distance of LZ surimi by 300% and 55%, respectively. Combination of 0.2% AsA and 0.15% H2 O2 resulted in the maximum TB surimi gel improvement with 150% and 90% increase in breaking force and distance, respectively. Browning reaction obviously occurred when combined AsA and H2 O2 was added, due to ascorbic acid oxidation. Polymerization of myosin heavy chain via disulfide bonds was promoted, and the formation of disulfide bonds was involved through oxidation of sulfhydryl groups with increasing AsA and H2 O2 . Fourier-transform infrared (FT-IR) spectroscopy revealed a decrease in α-helix and an increase in β-sheet content as AsA and H2 O2 increased in both species. A decrease of band area of aliphatic (2,800 to 3,000 and 1,450 cm-1 ), aromatic (1,208, 757, and ratio 850/830 cm-1 ), and change of disulfide bonds (525 and 540 cm-1 ) suggested an increase in hydrophobic interactions and disulfide bonds with addition of these additives. Based on principal component analysis (PCA), textural characteristics were positively correlated with β-sheet content. Our study suggested that combination of AsA and H2 O2 greatly enhanced gelation of LZ and TB by increasing not only disulfide bonds but also hydrophobic interactions. PRACTICAL APPLICATION: The combined ascorbic acid and hydrogen peroxide can be used to improve gelation of two important tropical surimi species, namely threadfin bream and lizardfish surimi, without requirement of setting. The optimum concentration of each additive varied with fish species.
- Published
- 2021
13. Meat quality and Raman spectroscopic characterization of Korat hybrid chicken obtained from various rearing periods
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Amonrat Molee, Kanjana Thumanu, Jirawat Yongsawatdigul, and Sasikan Katemala
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Market needs ,2019-20 coronavirus outbreak ,Meat ,Coronavirus disease 2019 (COVID-19) ,chicken ,Guanosine Monophosphate ,Spectrum Analysis, Raman ,Crossbreed ,meat quality ,principle component analysis ,Protein content ,Inosine Monophosphate ,Fourier transform Raman spectroscopy ,Animals ,Food science ,Spectral data ,lcsh:SF1-1100 ,Fourier Analysis ,business.industry ,Chemistry ,Fatty Acids ,Broiler ,Age Factors ,Proteins ,food and beverages ,General Medicine ,Processing and Products ,Poultry farming ,Hydrogen-Ion Concentration ,Cholesterol ,age ,Purines ,Animal Science and Zoology ,Collagen ,lcsh:Animal culture ,business ,Shear Strength ,Chickens - Abstract
Meat quality attributes vary with chicken age. Understanding the relationship between poultry age and the quality of the meat would be beneficial for efficient poultry farming to meet market needs. The Korat hybrid chicken (KC) is a new crossbred chicken whose meat quality is distinct from that of commercial broiler (CB) chickens and has not been well characterized. In this study, we characterized the physico-chemical properties of KC meat and correlate the findings with Raman spectral data. The protein content of KC breast and thigh meat increased with age. The pH of thigh meat decreased, while the water-holding capacity of breast meat increased as the age of the chickens increased. The amount of cholesterol in breast meat decreased as the rearing period was extended. Inosine 5′-monophosphate and guanosine 5′-monophosphate of breast meat decreased as KC grew older. The shear force values of meat from older birds increased concomitantly with an increase in total collagen. Principle component analysis revealed that the meat quality of CB was greatly different from that of KC meat. High shear force values of KC meat at 20 wk of age were well correlated with an increase in the β-sheet structure (amide I) and amide III of collagen. Raman spectra at 3,207 cm−1 and relative α-helical content were negatively correlated with shear force values of KC breast meat. These could be used as markers to evaluate KC meat quality.
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- 2021
14. Heating temperatures affect meat quality and vibrational spectroscopic properties of slow- and fast-growing chickens
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Sasikan Katemala, Amonrat Molee, Kanjana Thumanu, and Jirawat Yongsawatdigul
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Animal Science and Zoology ,General Medicine - Published
- 2023
15. Sensory Characteristics and Microbiological Quality Changes of Nile Tilapia Fillet Processed by Various Sous-vide Conditions During Chilled Storage
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Jaksuma Pongsetkul, Soottawat Benjakul, Surintorn Boonanuntanasarn, and Jirawat Yongsawatdigul
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Animal Science and Zoology ,Aquatic Science - Abstract
Sensory characteristics and microbiological quality of Nile tilapia cooked with various sous-vide (SV) conditions including 50 and 60°C, for 30, 45 and 60 min (S5-30, S5-45, S5-60, S6-30, S6-45 and S6-60) and their changes during storage (4°C) were investigated, compared with control (cooking with boiling water). The result found that increasing temperature and time of SV accelerated protein degradation, both myofibrillar protein and connective tissue. This affected to the lower water-holding capacity (WHC) and shear force of samples treated with severe SV conditions (S6-45 and S6-60) (P0.05). However, SV cooked samples had higher sensory scores than control, both at day 0 (before storage) or throughout the storage time. This suggested the potential to improve consumer acceptability by this technique. In this study, SV at 60°C, 60 min seem to be the optimal condition for tilapia fillet, which can preserve at 4℃ for at least 6 weeks without any spoilage.
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- 2022
16. Reduction of red blood spots in cooked marinated chicken breast meat by combined microwave heating and steaming
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Matthanee Jantaranikorn, Kanjana Thumanu, and Jirawat Yongsawatdigul
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Animal Science and Zoology ,General Medicine - Abstract
One of the defects commonly found in cooked marinated chicken breast products is a red blood spot (RBS), which is caused by undercooked blood in vessels. This problem was alleviated by microwave (MW) pre-heating for 6 to 7 min, followed by steaming. RBS formation decreased when samples were heated to a core temperature of 80°C and were completely eliminated at a core temperature of 82°C and 85°C when a MW pre-heating step was applied for 7 min. Based on synchrotron-based Fourier transform infrared spectroscopy (SR-FTIR), blood remaining in the blood vessel had a lower α-helical content when samples were cooked by the combination of MW heating and steaming as compared with those prepared by steaming alone (P0.05). MW pre-heating decreased cooking time by 28 to 48% as compared with steaming alone. Heating regimes had no effect on cooking loss, pH, water-holding capacity, and shear force. MW pre-heating for 7 min followed by steaming to a core temperature of 82°C appeared to be an effective heating regime to reduce the occurrence of RBS, with acceptable cooking loss.
- Published
- 2022
17. Biomolecules, Fatty Acids, Meat Quality, and Growth Performance of Slow-Growing Chickens in an Organic Raising System
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Wittawat Molee, Wichuta Khosinklang, Pramkamon Tongduang, Kanjana Thumanu, Jirawat Yongsawatdigul, and Amonrat Molee
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General Veterinary ,organic raising system ,meat quality ,omega-3 fatty acid ,slow-growing chicken ,synchrotron FTIR ,Animal Science and Zoology - Abstract
This study was to determine the effect of the organic raising system (OR) on growth performance, meat quality, and physicochemical properties of slow-growing chickens. Three hundred and sixty (one-day-old) Korat chickens (KRC) were randomly assigned to control (CO) and OR groups. The groups comprised six replicates of thirty chickens each. The chickens were housed in indoor pens (5 birds/m2), wherein those in OR had free access to Ruzi pasture (1 bird/4 m2) from d 21 to d 84 of age. In the CO group, chickens were fed with a mixed feed derived from commercial feedstuffs, while those in the OR group were fed with mixed feed derived from organic feedstuffs. The results revealed a lower feed intake (p < 0.0001) and feed conversion ratio (p = 0.004) in the OR. The OR increased total collagen, protein, shear force, color of skin and meat, and decreased abdominal fat (p < 0.05). The OR improved fatty acid with increased DHA, n-3 PUFA, and decreased the ratio of n-6 to n-3 PUFA in KRC meat (p < 0.05). The synchrotron radiation-based Fourier transform infrared spectroscopy and correlation loading analyses confirmed these results. In conclusion, our results proved that OR could improve growth performance and meat quality and suggested the raising system be adopted commercially. In addition, the observed differences in biochemical molecules could also serve as markers for monitoring meat quality.
- Published
- 2022
- Full Text
- View/download PDF
18. Physicochemical Properties and Angiotensin I Converting Enzyme Inhibitory Peptides of Freshwater Fish Skin Collagens
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Pornpimol Sungperm, Chompoonuch Khongla, and Jirawat Yongsawatdigul
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0106 biological sciences ,chemistry.chemical_classification ,food.ingredient ,integumentary system ,biology ,Chemistry ,Tilapia ,04 agricultural and veterinary sciences ,Aquatic Science ,biology.organism_classification ,Angiotensin I converting enzyme ,Inhibitory postsynaptic potential ,040401 food science ,01 natural sciences ,0404 agricultural biotechnology ,food ,Enzyme ,Biochemistry ,010608 biotechnology ,Inhibitory peptide ,Freshwater fish ,human activities ,Food Science ,Catfish - Abstract
The objectives of this study were to characterize physicochemical properties of two collagens, tilapia skin (TS) and hybrid catfish skin (HS). Angiotensin-converting enzyme (ACE) inhibitory peptide...
- Published
- 2020
19. Investigating the Effect of Various Sous-Vide Cooking Conditions on Protein Structure and Texture Characteristics of Tilapia Fillet Using Synchrotron Radiation-Based FTIR
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Jaksuma Pongsetkul, Supatcharee Siriwong, Kanjana Thumanu, Surintorn Boonanuntanasarn, and Jirawat Yongsawatdigul
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Health (social science) ,synchrotron FTIR ,principal component analysis ,sous vide ,Plant Science ,Nile tilapia ,texture ,Health Professions (miscellaneous) ,Microbiology ,Food Science - Abstract
The effects of various sous-vide (SV) cooking conditions (50-60℃, 30-60 min) on physicochemical properties related to the texture characteristics, protein structure/degradation, and sensory acceptability of tilapia fillet (Oreochromis niloticus) were investigated. With an increasing temperature and processing time of SV cooking, protein degradation (of both myofibrils and connective tissue) was more pronounced, as evaluated by the decrease in water- and salt-soluble proteins, total collagen, as well as the changes in the ratio of secondary protein structures (α-helix, β-sheet, β-turn, etc.), which were determined by synchrotron-FTIR (SR-FTIR). These degradations were associated with the improvement of meat tenderness, as estimated by shear force and texture profile analyzer (TPA) results. Among all SV conditions, using 60 ℃ for 45 min seems to be the optimal condition for tilapia meat, since it delivered the best results for texture characteristics and acceptability (p < 0.05). Moreover, principal component analysis (PCA) results clearly demonstrated that the highest texture-liking score of this condition was well associated with the intensity of β-sheets, which seem to be the crucial component that affected the texture of SV-cooked tilapia more so than other parameters. The findings demonstrated the potential of SR-FTIR to decipher the biomolecular structure, particularly the secondary protein structure, of SV-cooked tilapia. This technique provided essential information for a better understanding of the changes in biomolecules related to the textural characteristics of this product.
- Published
- 2023
20. Bioactivities of In Vitro Transepithelial Transported Peptides from Cooked Chicken Breast
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Ali Hamzeh, Papungkorn Sangsawad, Parinya Noisa, Kiattawee Choowongkomon, and Jirawat Yongsawatdigul
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Drug Discovery ,Molecular Medicine ,Bioengineering ,Biochemistry ,Analytical Chemistry - Published
- 2021
21. Development of Flavor and Taste Components of Sous-Vide-Cooked Nile Tilapia (Oreochromis niloticus) Fillet as Affected by Various Conditions
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Jaksuma Pongsetkul, Jirawat Yongsawatdigul, Surintorn Boonanuntanasarn, and Soottawat Benjakul
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Health (social science) ,sous-vide ,volatile compounds ,taste/flavor components ,Nile tilapia ,principal component analysis ,Plant Science ,Health Professions (miscellaneous) ,Microbiology ,Food Science - Abstract
This study aims to shed light on the association between non-volatile and volatile compounds related to flavor/taste characteristics as well as sensory acceptability of Nile tilapia fillet (Oreochromis niloticus) cooked by various sous-vide (SV) conditions (50–60 ℃, 30–60 min), with fish cooked with boiling water used as control. Higher temperatures and longer processing times of SV cooking led to greater protein and lipid oxidation as indicated by the increase in total sulfhydryl (-SH), carbonyl, free fatty acid (FFA) contents as well as peroxide values (PV) and thiobarbituric acid reactive substance (TBARS) values. The differences in flavor/taste components including adenosine triphosphate (ATP)-related compounds, free amino acids (FAAs) and volatiles were also obtained, which directly affect sensory acceptability as evaluated by using the hedonic scale. Based on principal component analysis (PCA) results, the acceptability score was strongly correlated with inosine monophosphate (IMP) and acetoin, which seem to be the most crucial flavor enhancers for cooked tilapia. Among all samples, tilapia processed at 60 °C for 45 and 60 min, which contained significantly higher IMP and acetoin (p < 0.05) than others, had significantly higher flavor-liking and overall-liking scores, with a more than 7.5 meaning for high acceptability (p < 0.05), indicating the optimal SV conditions for tilapia fillet. Overall, the present finding indicated that the SV-cooking technique, at the optimal conditions, can improve the meat quality of cooked fish, in terms of flavor/taste characteristics, compared with traditional cooking (control).
- Published
- 2022
22. Molecular Insights into the Mode of Action of Antibacterial Peptides Derived from Chicken Plasma Hydrolysates
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Fu Tian, Sureelak Rodtong, Kanjana Thumanu, Yanling Hua, Sittiruk Roytrakul, and Jirawat Yongsawatdigul
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Health (social science) ,antibacterial peptides ,SR-FTIR ,molecular docking ,Bacillus cereus ,Plant Science ,Health Professions (miscellaneous) ,Microbiology ,Food Science - Abstract
Due to the overuse and abuse of antibiotics, several antibiotic resistant bacteria have emerged. Antimicrobial peptides (AMPs) have gained attention as alternative antimicrobial agents because of their unique mode of action that impedes bacterial resistance. Two novel antibacterial peptides were isolated from Alcalase-hydrolyzed chicken plasma by size exclusion and reverse-phase chromatography. They were identified by LC-MS/MS to be VSDH and CCCPKAF, which showed effective antibacterial activity toward Bacillus cereus DMST 5040, with varied modes of action. The peptide CCCPKAF caused cell membrane disintegration, as evidenced by propidium iodide (PI) uptake. In contrast, the peptide VSDH targeted intracellular molecules, including proteins and nucleic acids, as revealed by Synchrotron-based Fourier Transform Infrared (SR-FTIR). The secondary structure of intracellular proteins increased to a β-sheet structure concomitant with a decrease in the α-helix structure when exposed to 0.5 mM VSDH. Molecular docking analysis revealed that VSDH showed high binding affinity for the active sites of the various enzymes involved in DNA synthesis. In addition, it showed good affinity for a chaperone protein (Dnak), resulting in the misfolding of intracellular proteins. Nuclear magnetic resonance (NMR) and molecular dynamics simulations also indicated that VSDH chelated well with Mg2+, which could partly contribute to its antibacterial activity.
- Published
- 2022
23. Effects of β-alanine and L-histidine supplementation on carnosine contents in and quality and secondary structure of proteins in slow-growing Korat chicken meat
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Chanadda Suwanvichanee, Panpradub Sinpru, Kasarat Promkhun, Satoshi Kubota, Cindy Riou, Wittawat Molee, Jirawat Yongsawatdigul, Kanjana Thumanu, and Amonrat Molee
- Subjects
Meat ,Carnosine ,Dietary Supplements ,beta-Alanine ,Animals ,Animal Science and Zoology ,Female ,Histidine ,General Medicine ,Anserine ,Muscle, Skeletal ,Chickens - Abstract
Carnosine enrichment of slow-growing Korat chicken (KRC) meat helps differentiate KRC from mainstream chicken. We aimed to investigate the effects of β-alanine and L-histidine supplementation on the carnosine synthesis in and quality and secondary structure of proteins in slow-growing KRC meat. Four hundred 21-day-old female KRC were used, and a completely randomized design was applied. The chickens were divided into 4 experimental groups: basal diet (A), basal diet supplemented with 1.0% β-alanine (B), 0.5% L-histidine (C), and 1.0% β-alanine combined with 0.5% L-histidine (D). Each group consisted of 5 replicates (20 chickens per replicate). On d 70, 2 chickens per replicate were slaughtered, and the levels of carnosine, anserine, and thiobarbituric acid reactive substances were analyzed. Biochemical changes were monitored using synchrotron radiation-based Fourier transform infrared microspectroscopy; 5 chickens per replicate were slaughtered, and the meat quality was analyzed. Statistical analysis was performed using ANOVA and principal component analysis (PCA). Group D chickens exhibited the highest carnosine meat content, followed by those in groups B and C. However, amino acid supplementation did not affect anserine content and growth performance. Higher carnosine levels correlated with increasing pH
- Published
- 2021
24. Comparative proteomics revealed duodenal metabolic function associated with feed efficiency in slow-growing chicken
- Author
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Pramin Kaewsatuan, Chotima Poompramun, Satoshi Kubota, Jirawat Yongsawatdigul, Wittawat Molee, Pekka Uimari, Amonrat Molee, Helsinki One Health (HOH), Department of Agricultural Sciences, Animal Science Research, and University of Helsinki
- Subjects
Slow-growing chicken ,Male ,Proteomics ,Proteome ,Duodenum ,Feed efficiency ,General Medicine ,Label-free proteomics ,Animals ,Animal Science and Zoology ,412 Animal science, dairy science ,Chickens ,Metabolic Networks and Pathways ,Korat chicken - Abstract
The Korat chicken (KR), developed in Thailand, is a slow-growing breed developed as an alternative breed for Thai chicken producers. The growing interest in slow-growing chicken meat, due to its unique taste, distinct texture, health benefits, and higher broiler welfare have led to higher market demand for KR. However, its low feed efficiency (FE) has a significant negative impact on farm profitability. Understanding the molecular mechanism regulating FE allows for designing a suitable selection program and contributing to breeding more efficient chicken for poultry production. Thus, the objective of our study was to investigate the proteome differences and possible pathways associated with FE in male KR using a label-free quantitative proteomic approach. Seventy-five KR males were individually evaluated for FE, and duodenum samples from 6 animals (3 high-FE and 3 low-FE chickens) were collected at 10 wk of age for differential abundant proteins (DAPs), protein networks, functional enrichment, and pathway analyses. In this study, we found 40 DAPs significantly associated with FE pathways, including glycolysis/gluconeogenesis, peroxisome, oxidative phosphorylation, tight junction, and cysteine and methionine metabolism. Thus, variations in observed DAPs or genes related to DAPs could be interesting biomarker candidates for selection for higher feed utilization efficiency in chicken.
- Published
- 2021
25. Bioactive Peptides from Agriculture and Food Industry Co-Products: Peptide Structure and Health Benefits
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Jirawat Yongsawatdigul and Ali Hamzeh
- Subjects
0303 health sciences ,03 medical and health sciences ,0404 agricultural biotechnology ,Food industry ,030309 nutrition & dietetics ,business.industry ,Agriculture ,04 agricultural and veterinary sciences ,Business ,Health benefits ,Peptide structure ,040401 food science ,Biotechnology - Abstract
Co-products from food processing are typically disposed or turned into low value animal feed. Proteinaceous co-products can be converted to bioactive peptides exerting health benefits, which can lead to development of nutraceuticals and functional foods. This is an effective means for valorization of these co-products. The release of encrypted peptides exhibits various bioactivities, including antihypertension, antioxidant, immunomodulatory activities among others, in vitro, and some activities have been demonstrated in vivo. Structure modification of bioactive peptides occurring under gastrointestinal digestion and cellular transport remains the important factor determining the health benefits of bioactive peptides. Understanding peptide transformation in gastrointestinal tract and in blood circulation before reaching the target organs would shed some lights on its bioavailability and subsequently ability to exert physiological impact. In this chapter, the potential health promoting properties of peptides encrypted in various sources of co-products will be reviewed based on evidence on in vitro, in vivo and clinical trial studies. Structural changes of bioactive peptides under physiological condition will also be discussed in relation to its bioactivities.
- Published
- 2021
26. A comparative study of meat quality and vibrational spectroscopic properties of different chicken breeds
- Author
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Sasikan Katemala, Amonrat Molee, Kanjana Thumanu, and Jirawat Yongsawatdigul
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Meat ,Inosine Monophosphate ,Spectrum Analysis ,Animals ,Animal Science and Zoology ,General Medicine ,Chickens ,Lipids ,Inosine - Abstract
Chicken breed is one of the key factors that influence meat quality. The quality attributes of breast meat from commercial broiler (CB), Thai native chicken (NC, Leung Hang Khao), and the crossbred Korat chicken (KC) were investigated via synchrotron radiation-based Fourier transform infrared (SR-FTIR) microspectroscopy, Fourier transform Raman (FT-Raman) spectroscopy, and physicochemical analysis. The protein and carbonyl contents of KC and NC meats were higher than that of CB meat, but the lipid content was lower (P0.05). CB meat was characterized by high moisture, lightness (L*), and presence of taste-active nucleotides, namely, inosine 5'-monophosphate (IMP) and guanosine 5'-monophosphate (GMP). Moreover, NC meat had the highest insoluble collagen and inosine contents (P0.05). The predominant protein secondary structures of KC and NC meats were β-turns and random coils, whereas α-helices were mainly found in CB meat. Based on principal component analysis, the meat quality and spectra were clearly separated by breeds. The high moisture and lipid content of meat corresponded to O-H stretching (3,203 cm
- Published
- 2022
27. Evaluation of Lipid Oxidation, Volatile Compounds and Vibrational Spectroscopy of Silver Carp (Hypophthalmichthys molitrix) During Ice Storage as Related to the Quality of Its Washed Mince
- Author
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Kanjana Thumanu, Sasinee Kunyaboon, Jirawat Yongsawatdigul, Chompoonuch Khongla, and Jae W. Park
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Health (social science) ,Phospholipid ,Plant Science ,lcsh:Chemical technology ,01 natural sciences ,Health Professions (miscellaneous) ,Microbiology ,Hexanal ,Article ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Lipid oxidation ,lipid oxidation ,TBARS ,lcsh:TP1-1185 ,Food science ,volatile compounds ,chemistry.chemical_classification ,Silver carp ,Hypophthalmichthys ,biology ,010401 analytical chemistry ,04 agricultural and veterinary sciences ,Phosphate ,biology.organism_classification ,silver carp ,040401 food science ,0104 chemical sciences ,washed mince ,chemistry ,FTIR ,Food Science ,Polyunsaturated fatty acid ,FT-Raman - Abstract
Changes in the lipid oxidation of silver carp (Hypophthalmichthys molitrix) stored in ice for 14 days and that of its respective washed mince were evaluated. Total lipid, phospholipid, polyunsaturated fatty acid (PUFA) and monounsaturated fatty acid (MUFA) contents of the skin, belly flap and mince decreased as the storage time in ice increased. The washing process decreased the lipid contents but concentrated their phospholipid counterparts. The fish belly flap exhibited the highest thio-barbituric acid reactive substances (TBARS) value, while the mince had the lowest. 1-Hexanol, 1-octen-3-ol, and 1-hexanal were key volatile compounds detected in the belly flaps of fish stored for 7–14 days. Hexanal was the only major volatile compound found in washed mince prepared from fish stored for an extended period in ice, but in a much lower amount compared with that in the belly flap. FTIR (Fourier transform infra-red) spectra revealed a decrease in the number of cis double bonds, methylene groups and phosphate groups in lipids extracted from fish stored in ice for 7–14 days as compared with those extracted from fresh fish. Principle component analysis (PCA) revealed that the FT-Raman band at 1747 cm−1 could be a potential marker for tracking the degree of lipid oxidation in the belly flap of silver carp stored in ice. In addition, IR bands indicating phosphate group (925, 825 cm−1) in oil extracted from washed mince were correlated with the extent of the lipid oxidation of the raw material.
- Published
- 2021
- Full Text
- View/download PDF
28. High-intensity ultrasound improves threadfin bream surimi gelation at low NaCl contents
- Author
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Jirawat Yongsawatdigul and Ling Tang
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Fish Proteins ,030309 nutrition & dietetics ,Food Handling ,Protein Conformation ,Sodium Chloride ,03 medical and health sciences ,0404 agricultural biotechnology ,Low salt ,Fish Products ,Spectroscopy, Fourier Transform Infrared ,Atpase activity ,Animals ,Food science ,Sulfhydryl Compounds ,0303 health sciences ,biology ,business.industry ,Chemistry ,Nemipterus ,High intensity ,Ultrasound ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,Sea Bream ,Seafood ,Ultrasonic Waves ,Threadfin bream ,business ,Gels ,Hydrophobic and Hydrophilic Interactions ,Food Science - Abstract
Effects of high-intensity ultrasound (HIU) treatments on gelation of threadfin bream (Nemipterus spp.) surimi at various NaCl contents (0.5, 1, and 2% NaCl) were investigated. Protein extractability at 0.5% NaCl was increased with the ultrasonic intensity (p
- Published
- 2020
29. Effect of marinating ingredients on temperature-induced denaturation of hemoglobin and its relation to red blood spot formation in cooked chicken breast
- Author
-
Jirawat Yongsawatdigul and Matthanee Jantaranikorn
- Subjects
Thermal denaturation ,Protein Denaturation ,Meat ,Vacuum ,030309 nutrition & dietetics ,Sodium ,Marination ,chemistry.chemical_element ,Absorption (skin) ,Sodium Chloride ,Poultry ,Chicken breast ,03 medical and health sciences ,Hemoglobins ,0404 agricultural biotechnology ,Polyphosphates ,Animals ,Humans ,Denaturation (biochemistry) ,Food science ,Cooking ,0303 health sciences ,Temperature ,04 agricultural and veterinary sciences ,040401 food science ,Temperature induced ,Glucose ,chemistry ,Hemoglobin ,Chickens ,Food Science - Abstract
Red blood spot (RBS) commonly found in cooked chicken breast has caused severe economic loss as it is perceived as a sign of undercooked product. The objectives of this study were to investigate the cause of RBS as related to common ingredients used in marination, based on both chicken breast and isolated chicken hemoglobin (Hb) models. The effect of sodium chloride (NaCl), sodium tripolyphosphate (STPP), and glucose on thermal denaturation of Hb was investigated along with the extent of RBS formation in cooked marinated chicken breast. After vacuum tumbling for 65 min and subsequent storage at 4 °C for 20 hr, STPP and glucose were not absorbed into the center of chicken breast. However, Na+ was absorbed after 12 hr storage. The denaturation temperature (Td ) of isolated chicken Hb decreased to 65.8 °C in the presence of 1.5 M NaCl, while that of the control was 69.4 °C. STPP at pH 9 decreased Td of Hb to 61.4 °C. The alkaline pH induced by STPP destabilized the Hb structure. RBSs were observed at 100% incidence when cooked to core temperatures of 50 and 70 °C for 1 min. RBSs were completely eliminated at core temperature of 85 °C. The ingredients used during marination appeared to have a minimal effect on RBS formation due to their limited absorption into the chicken breast. The cooking temperature is a major factor governing RBSs, as it directly affects the denaturation of Hb.
- Published
- 2020
30. Ca2+- and Mg2+-Induced Conformational and Rheological Changes of Actomyosin Extracted from Fresh and Freeze-Thaw Tilapia
- Author
-
Jirawat Yongsawatdigul and Sornchai Sinsuwan
- Subjects
Actomyosins ,food.ingredient ,biology ,Magnesium ,chemistry.chemical_element ,Tilapia ,04 agricultural and veterinary sciences ,Aquatic Science ,Calcium ,biology.organism_classification ,040401 food science ,Oreochromis ,0404 agricultural biotechnology ,food ,chemistry ,Rheology ,sense organs ,Food science ,skin and connective tissue diseases ,human activities ,Food Science - Abstract
The conformational changes of natural actomyosins prepared from fresh and freeze-thaw tilapia (Oreochromis niloticus) in the presence of Ca2+ or Mg2+ were investigated. The Ca2+-activated a...
- Published
- 2018
31. Effects of different NaCl concentrations on self-assembly of silver carp myosin
- Author
-
Jirawat Yongsawatdigul, Liu Manman, Shanbai Xiong, Guan Wang, Liwei Cao, and Ru Liu
- Subjects
Silver carp ,biology ,Chemistry ,Intermolecular force ,macromolecular substances ,04 agricultural and veterinary sciences ,biology.organism_classification ,Microstructure ,040401 food science ,Biochemistry ,Hydrophobic effect ,0404 agricultural biotechnology ,Particle-size distribution ,Myosin ,Biophysics ,Self-assembly ,Solubility ,Food Science - Abstract
The effect of NaCl (0.1 − 3.0 M) on self-assembly of silver carp myosin at 4 °C was investigated in terms of microstructure, conformation, intermolecular interactions, and particle size distribution. During setting at 4 °C, the self-assembly of myosin showed an obvious concentration dependence. At low concentrations ( 2+ -ATPase activity reached a maximum at 0.6 M NaCl. When the concentrations increased to >1.0 M, myosin further assembled into filaments dominated by hydrophobic interactions. Both rod-rod and head-head interactions contributed to the myosin filaments at high concentrations (1.0 − 3.0 M). The solubility and uniformity of the myosin assemblies decreased as the NaCl concentration increased from 1.0 to 3.0 M.
- Published
- 2018
32. Production and characterization of chicken blood hydrolysate with antihypertensive properties
- Author
-
Soichiro Nakamura, Jirawat Yongsawatdigul, Takakazu Mitani, Shigeru Katayama, and Wasana Wongngam
- Subjects
hypertension ,Protein Hydrolysates ,Angiotensin-Converting Enzyme Inhibitors ,Blood Pressure ,Hydrolysate ,Hydrolysis ,Oral administration ,spontaneously hypertensive rat (SHR) ,Renin–angiotensin system ,Animals ,Food science ,Antihypertensive Agents ,lcsh:SF1-1100 ,chemistry.chemical_classification ,General Medicine ,Blood Proteins ,Processing and Products ,angiotensin I-converting enzyme (ACE) ,Amino acid ,Rats ,Disease Models, Animal ,Enzyme ,Blood pressure ,chemistry ,Animal Science and Zoology ,lcsh:Animal culture ,Digestion ,chicken blood ,Chickens ,protein hydrolysate - Abstract
Chicken blood has limited utilization despite its high protein content. Production of a blood hydrolysate exhibiting angiotensin I-converting enzyme (ACE)–inhibitory activity would be means of valorizing chicken blood. The optimized conditions used to produce chicken blood corpuscle hydrolysate (BCH) by Alcalase were 51.1°C, 4% enzyme, and pH 9.6 for 6 h, resulting in a 35.8% degree of hydrolysis and 37.7% ACE inhibition at a peptide concentration of 0.2 mg/mL. The permeate of a 1-kDa membrane, BCH-III, showed a 2.5-fold increase in ACE inhibition compared with that of BCH. BCH-III was resistant to in vitro gastrointestinal digestion, whereas the BCH digesta exhibited an increased ACE-inhibitory activity after digestion. Both BCH and BCH-III were rich in hydrophobic amino acids. A single administration of BCH and BCH-III to spontaneously hypertensive rats at concentrations of 600 and 100 mg/kg, respectively, lowered the systolic blood pressure by −57.7 and −70.9 mmHg, respectively, 6 h after oral administration compared with the control group. The blood pressure–lowering effect of the 600 mg/kg BCH dose was comparable with that of the 100 mg/kg BCH-III dose after 4 wk of oral administration. Both BCH and BCH-III could be developed for use as nutraceutical products with antihypertensive effects.
- Published
- 2019
33. Transepithelial transport and structural changes of chicken angiotensin I-converting enzyme (ACE) inhibitory peptides through Caco-2 cell monolayers
- Author
-
Xiu-Min Chen, Jirawat Yongsawatdigul, Kiattawee Choowongkomon, Eunice C.Y. Li-Chan, David D. Kitts, and Papungkorn Sangsawad
- Subjects
0301 basic medicine ,Medicine (miscellaneous) ,Permeability ,Bioactive peptide ,03 medical and health sciences ,Renin–angiotensin system ,medicine ,TX341-641 ,IC50 ,chemistry.chemical_classification ,030109 nutrition & dietetics ,Nutrition and Dietetics ,biology ,Chemistry ,Nutrition. Foods and food supply ,Active site ,Angiotensin I-converting enzyme ,In vitro ,3. Good health ,Enzyme ,Biochemistry ,Caco-2 ,Peptide transport ,ACE inhibitor ,Transepithelial transport ,Molecular docking ,biology.protein ,Food Science ,medicine.drug - Abstract
Permeability of angiotensin I-converting enzyme (ACE) inhibitory peptides (KPLLCS, ELFTT, and KPLL) identified from the in vitro gastrointestinal digest of cooked chicken breast was investigated using the Caco-2 cell model system. KPLLCS was originally the most effective ACE inhibitor (IC50 0.37 μM), but it was degraded during permeation through Caco-2 cells. Among these peptides, KPLL showed the highest permeability in intact form, and partially degraded to KP and LL, which still showed ACE inhibitory activity after permeation. ACE inhibitory activity of permeated KPLL was highest of 56%. KPLL and KP possessed a mixed inhibitor characteristic, while LL was a non-competitive inhibitor. Based on molecular docking, K at N-terminus of KPLL is a key structure contributing to ACE inhibition as it can occupy the active site of ACE. Determining the structural stability and degree of peptide transport across epithelial cell is required to confirm their potential as ACE inhibitors.
- Published
- 2018
34. Silver Carp Bone Powder as Natural Calcium for Fish Sausage
- Author
-
Jirawat Yongsawatdigul, Jaruwan Siritapetawee, Wanwisa Limphirat, Koo Bok Chin, and Bung-Orn Hemung
- Subjects
0301 basic medicine ,Silver carp ,030109 nutrition & dietetics ,biology ,Chemistry ,chemistry.chemical_element ,04 agricultural and veterinary sciences ,Aquatic Science ,Calcium ,biology.organism_classification ,040401 food science ,03 medical and health sciences ,Ingredient ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Sodium hydroxide ,Natural source ,%22">Fish ,sense organs ,Food science ,Food Science ,Fish bone - Abstract
Although silver carp bones (SCB) are generated as waste, they are a natural source of calcium and have the potential to be a food ingredient. When the SCB had been soaked in hot sodium hydroxide (0...
- Published
- 2018
35. Identification and characterization of tilapia antioxidant peptides that protect AAPH-induced HepG2 cell oxidative stress
- Author
-
Jirawat Yongsawatdigul, Parinya Noisa, and Xiaogang Zhang
- Subjects
Antioxidant ,food.ingredient ,medicine.medical_treatment ,Medicine (miscellaneous) ,Peptide ,medicine.disease_cause ,Hydrolysate ,chemistry.chemical_compound ,Nutraceutical ,food ,medicine ,TX341-641 ,chemistry.chemical_classification ,Nutrition and Dietetics ,ABTS ,Nutrition. Foods and food supply ,Chemistry ,Reactive oxygen species (ROS) ,In silico digestion ,Tilapia ,Ascorbic acid ,Biochemistry ,Gene expression ,Oxidative stress ,Food Science - Abstract
Nine novel peptides from tilapia hydrolysate were isolated and identified to be EKL, EKP, HKPA, ELSC, ALSC, ASLCH, SLCH, LPGYF, and LEVPGY. In addition, six fragments from in silico gastrointestinal digestion of the identified peptides, including EK, KPA, SC, CH, PGY, and EVPGY, were synthesized. The most effective parent and digested peptides showing ABTS · + scavenging capacity were LPGYF and SC, respectively. All C- and Y-containing peptides were more effective than ascorbic acid (AsA). In contrast, K-containing peptides exhibited less antioxidant activity. All 15 peptides showed potent intracellular reactive oxygen species scavengers in the AAPH-induced HepG2 cell oxidative stress model. In addition, the digested peptides SC, CH, and PGY up-regulated the expression of CAT and SOD1 in HepG2 cells. The peptide PGY was the most effective cellular antioxidant. Thus, tilapia peptides could be potent nutraceutical products to reduce cellular oxidative stress.
- Published
- 2021
36. Effect of ultrasound pre-treatment modes on gelation properties of silver carp surimi
- Author
-
Hongying Du, Shanbai Xiong, Runlin Wu, Juan You, Jirawat Yongsawatdigul, Ru Liu, and Xia Gao
- Subjects
0106 biological sciences ,Pre treatment ,Silver carp ,biology ,Chemistry ,High intensity ,04 agricultural and veterinary sciences ,Protein degradation ,biology.organism_classification ,040401 food science ,01 natural sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Chemical engineering ,010608 biotechnology ,Solubility ,Polyvinylidene chloride ,Dispersion (chemistry) ,Food Science - Abstract
The aim of this study was to illustrate the effect of high intensity ultrasound (HIU) pre-treatment modes (HIU pre-treatment before salt-chopping (HIUBC), after salt-chopping (HIUAC) and after stuffing into polyvinylidene chloride casing (HIUAS)) on the gelation properties of silver carp surimi. HIUBC facilitated the protein dispersion and more compact microstructures formation, which was beneficial to obtaining the highest water-holding capacity (WHC) of HIUBC gels. Meanwhile, the puncture properties of HIUBC gels were significantly improved via the formation of more e-(γ-Glu)-Lys and S–S bonds. HIUAC and HIUAS promoted the formation of e-(γ-Glu)-Lys bonds, but also induced the protein degradation, as evidenced by the decreased solubility and relatively higher TCA-soluble peptides. Thus, no obvious improvement of puncture properties and WHC was observed for HIUAC or HIUAS gels. In conclusion, HIUBC was beneficial to facilitating protein dispersion, thereby improving gelation properties of surimi during heat-induced gelling process. However, HIUAC and HIUAS hardly influenced gelation properties of surimi. This might provide an effective method of HIU pre-treatment for promoting gelation properties of surimi.
- Published
- 2021
37. Immunomodulatory activity of protein hydrolysates derived from Virgibacillus halodenitrificans SK1-3-7 proteinase
- Author
-
Tidarat Toopcham, Jurriaan J. Mes, Jirawat Yongsawatdigul, and Harry J. Wichers
- Subjects
Fish Proteins ,Lipopolysaccharides ,0301 basic medicine ,Protein Hydrolysates ,Interleukin-1beta ,Anti-Inflammatory Agents ,Hydrolysate ,Analytical Chemistry ,Immunomodulation ,03 medical and health sciences ,Hydrolysis ,Virgibacillus ,Casein ,Gene expression ,Animals ,Humans ,Interleukin 8 ,Cells, Cultured ,Food, Health & Consumer Research ,VLAG ,Plant Proteins ,Inflammation ,030109 nutrition & dietetics ,biology ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Chemistry ,Macrophages ,Pea protein ,Interleukin-8 ,Peas ,Caseins ,Cichlids ,General Medicine ,Protein hydrolysate ,biology.organism_classification ,Immunity, Innate ,Health & Consumer Research ,Gene Expression Regulation ,Biochemistry ,Food ,Cyclooxygenase 2 ,Tumor necrosis factor alpha ,Peptide Hydrolases ,Food Science - Abstract
Modulation of inflammation-related immune response on THP-1 macrophages of protein hydrolysates derived from tilapia mince, casein and pea protein, were investigated. The protein substrates were hydrolyzed by Virgibacillus halodenitrificans SK1-3-7 proteinase. The degree of hydrolysis (DH) of casein was observed to be the highest throughout the course of hydrolysis. When challenging THP-1 macrophages, tilapia mince hydrolysate (TMH) enhanced innate immunity through induction of IL-1β and COX-2 expression. Anti-inflammatory activity was observed in casein hydrolysate (CH) and pea protein hydrolysate (PPH) by attenuating lipopolysaccharide- (LPS) induced pro-inflammatory gene expression in THP-1 macrophages. CH suppressed IL-1β, IL-6, IL-8, TNF-α and COX-2, while PPH reduced LPS-induced IL-6 and TNF-α responses. In addition, CH and PPH showed stronger suppression of LPS-induced pro-inflammatory gene expression compared with non-hydrolyzed casein and pea protein. These results suggest that TMH, CH and PPH prepared from V. halodenitrificans SK1-3-7 proteinase are potential functional food ingredients with immunomodulatory activity.
- Published
- 2017
38. Improvement of fish sauce quality by combined inoculation of Tetragenococcus halophilus MS33 and Virgibacillus sp. SK37
- Author
-
Sureelak Rodtong, Shu Chen, Natteewan Udomsil, and Jirawat Yongsawatdigul
- Subjects
biology ,Inoculation ,Virgibacillus sp ,04 agricultural and veterinary sciences ,Glutamic acid ,biology.organism_classification ,040401 food science ,0404 agricultural biotechnology ,Starter ,Tetragenococcus halophilus ,Virgibacillus ,%22">Fish ,Fermentation ,Food science ,Food Science ,Biotechnology - Abstract
Biological and chemical dynamics were investigated during fermentation of fish sauce inoculated single and combined starter cultures, Virgibacillus sp. SK37 and Tetragenococcus halophilus MS33. At 120–150 days of fermentation, cell numbers of Virgibacillus sp. SK37 and T. halophilus MS33 were lower as detected by qPCR in fish sauce inoculated with a single culture compared with the sample inoculated with combined cultures. In addition, qPCR revealed that the sequential inoculation of Virgibacillus sp. SK37 followed by the addition of T. halophilus MS33 after 1 month (SK37_1M + MS33) showed bacterial counts that were approximately 0.5–1 LogCFU/mL higher than the single starter culture treatments. α-Amino contents of fish sauce added simultaneous co-cultures (MS33 + SK37) and SK37_1M + MS33 were 1160.41 and 1185.71 mM, respectively. SK37_1M + MS33 showed a lower amount of histamine than the control ( P Virgibacillus sp. SK37 or T. halophilus MS33 showed higher amounts of total glutamic acid and greater total amounts of amino acids than the control ( P , P
- Published
- 2017
39. Angiotensin converting enzyme (ACE) inhibitory peptides derived from the simulated in vitro gastrointestinal digestion of cooked chicken breast
- Author
-
Sittiruk Roytrakul, Papungkorn Sangsawad, and Jirawat Yongsawatdigul
- Subjects
Plasmin ,Medicine (miscellaneous) ,Chicken breast ,Bioactive peptide ,Hydrolysis ,0404 agricultural biotechnology ,Myosin ,medicine ,TX341-641 ,Nutrition and Dietetics ,biology ,Chemistry ,Nutrition. Foods and food supply ,Broiler ,In vitro digestion ,Angiotensin-converting enzyme ,04 agricultural and veterinary sciences ,040401 food science ,In vitro ,Biochemistry ,ACE inhibitor ,biology.protein ,Digestion ,Angiotensin converting enzyme ,Food Science ,medicine.drug - Abstract
The release of encrypted angiotensin converting enzyme (ACE) inhibitory peptides upon simulated in vitro gastrointestinal (GI) digestion of Korat crossbred (KC) and commercial broiler (BR) breast meat varied with cooking methods. Digestas of KC breast meat heated at 70 °C for 0.5 h (H-0.5) showed the highest ACE inhibitory activity, while digestas of KC chicken heated at 121 °C for 1 h showed the lowest inhibitory activity. ACE inhibitory activity varied with the protein digestibility. After a series of chromatographic techniques, we identified KPLLCS, ELFTT, and KPLL, novel potent ACE inhibitor peptides whose sequences were homologous to that of myosin and whose IC50 values were 0.37, 6.35 and 11.98 μM, respectively. ELFTT and KPLL were resistant to plasmin hydrolysis for up to 60 min. Therefore, KC chicken meat heated at H-0.5 could serve as a source of ACE inhibitory peptides upon GI digestion.
- Published
- 2017
40. Physicochemical properties of tilapia (Oreochromis niloticus) actomyosin subjected to high intensity ultrasound in low NaCl concentrations
- Author
-
Ling Tang and Jirawat Yongsawatdigul
- Subjects
food.ingredient ,Acoustics and Ultrasonics ,02 engineering and technology ,Calcium-Transporting ATPases ,Sodium Chloride ,010402 general chemistry ,01 natural sciences ,Inorganic Chemistry ,Protein content ,Sonication ,food ,Chemical Engineering (miscellaneous) ,Environmental Chemistry ,Animals ,Radiology, Nuclear Medicine and imaging ,Food science ,Sulfhydryl Compounds ,biology ,business.industry ,Chemistry ,High intensity ,Organic Chemistry ,Ultrasound ,Tilapia ,Actomyosin ,021001 nanoscience & nanotechnology ,biology.organism_classification ,0104 chemical sciences ,Intensity (physics) ,Oreochromis ,0210 nano-technology ,business - Abstract
Effects of high intensity ultrasound (HIU) on physicochemical properties of tilapia (Oreochromis niloticus) actomyosin in low NaCl concentrations were investigated. The protein content extracted in low NaCl concentrations (0.1–0.3 M NaCl) increased with increasing HIU intensity up to 20.62 W/cm2 (p
- Published
- 2019
41. Cellular and chemical antioxidant activities of chicken blood hydrolysates as affected by in vitro gastrointestinal digestion
- Author
-
Ali Hamzeh, Wasana Wongngam, Jirawat Yongsawatdigul, and Ratana Kiatsongchai
- Subjects
animal structures ,Antioxidant ,Protein Hydrolysates ,medicine.medical_treatment ,In Vitro Techniques ,Hydrolysate ,Antioxidants ,03 medical and health sciences ,chemistry.chemical_compound ,Pepsin ,Thermolysin ,Blood plasma ,medicine ,Animals ,Food science ,030304 developmental biology ,0303 health sciences ,ABTS ,biology ,Chemistry ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,040201 dairy & animal science ,Red blood cell ,medicine.anatomical_structure ,biology.protein ,Animal Science and Zoology ,Animal Nutritional Physiological Phenomena ,Digestion ,Chickens - Abstract
The effects of in vitro gastrointestinal (GI) digestion on the antioxidative activity of hydrolysates prepared from chicken blood plasma and red blood cell (RBC) by pepsin and thermolysin were investigated. The pepsin-hydrolyzed plasma (PHP) showed the highest scavenging activity of ABTS radicals (P < 0.05). RBC and plasma hydrolysates prepared by pepsin were hydrolyzed by GI proteases to a greater extent than hydrolysates prepared by thermolysin as evidenced by MALDI-TOF mass spectra. The antioxidative activity of all digesta increased compared to their respective parent hydrolysates, and PHP digesta showed the highest activity (P < 0.05). The digesta of PHP and thermolysin-hydrolyzed plasma showed cytoprotective properties in a dose-dependent manner, and 100 μg/mL of PHP digesta exhibited the highest protection of HepG2 cells against tert-butyl hydroperoxide (P < 0.05). Based on dichloro-dihydro-fluorescein diacetate assay, PHP digesta exhibited the greatest intracellular reactive oxygen species scavenging activity of approximately 71% at 100 μg/mL (P < 0.05). The peptide sequencing of PHP digesta revealed that they contained less than 10 amino acid residues, with an average hydrophobicity of 18.6. Chicken blood plasma is a better protein source for protein hydrolysates, and their digesta showed higher antioxidant activity compared to RBCs.
- Published
- 2018
42. Chemical and Cellular Antioxidant Activities of Chicken Breast Muscle Subjected to Various Thermal Treatments Followed by Simulated Gastrointestinal Digestion
- Author
-
Jirawat Yongsawatdigul, Papungkorn Sangsawad, Benjamart Chitsomboon, and Ratana Kiatsongchai
- Subjects
Metal chelating activity ,animal structures ,Antioxidant ,Chemistry ,medicine.medical_treatment ,0402 animal and dairy science ,Broiler ,04 agricultural and veterinary sciences ,040401 food science ,040201 dairy & animal science ,In vitro ,Gastrointestinal digestion ,0404 agricultural biotechnology ,Protein digestibility ,medicine ,Food science ,Digestion ,Intracellular ,Food Science - Abstract
The effect of thermal treatments on chemical and cellular antioxidant activities of chicken breasts subjected to in vitro gastrointestinal digestion was investigated. Breast of Korat crossbred chicken (KC) and commercial broiler (BR) were cooked under various conditions, namely heating at 70 °C for 30 min (H-0.5) and 24 h (H-24), autoclaving (AC) at 121°C for 15 min (AC-15) and 60 min (AC-60). Protein digestibility decreased upon the extreme thermal treatment of AC-60. The H-0.5 improved metal chelating activity of KC digesta, FRAP, and anti-liposome oxidation of BR digesta. Digesta of BR/H-0.5 and KC/AC-15 at 50 μg/mL exhibited the highest cytoprotective effect against tert-butyl hydroperoxide (TBHP)-induced oxidative damage of HepG2 cells. In addition, the KC/AC-15 digesta at a concentration as low as 12.5 μg/mL inhibited intracellular TBHP-induced reactive oxyfen species (ROS) production (P < 0.05). Thus, the digesta of KC breasts subjected to AC-15 provides not only nutritional value but also antioxidant activity at the cellular level.
- Published
- 2016
43. Quantification of viable bacterial starter cultures of Virgibacillus sp. and Tetragenococcus halophilus in fish sauce fermentation by real-time quantitative PCR
- Author
-
Jirawat Yongsawatdigul, Natteewan Udomsil, Shu Chen, and Sureelak Rodtong
- Subjects
0301 basic medicine ,biology ,030106 microbiology ,Enterococcaceae ,Fishes ,Real-Time Polymerase Chain Reaction ,Fish products ,biology.organism_classification ,Microbiology ,03 medical and health sciences ,030104 developmental biology ,Starter ,Propidium monoazide ,Virgibacillus ,Fermentation ,Fish Products ,Tetragenococcus halophilus ,Animals ,Food science ,Bacteria ,Food Science - Abstract
Real-time quantitative polymerase chain reaction (qPCR) methods were developed for the quantification of Virgibacillus sp. SK37 and Tetragenococcus halophilus MS33, which were added as starter cultures in fish sauce fermentation. The PCR assays were coupled with propidium monoazide (PMA) treatment of samples to selectively quantify viable cells and integrated with exogenous recombinant Escherichia coli cells to control variabilities in analysis procedures. The qPCR methods showed species-specificity for both Virgibacillus halodenitrificans and T. halophilus as evaluated using 6 reference strains and 28 strains of bacteria isolated from fish sauce fermentation. The qPCR efficiencies were 101.1% for V. halodenitrificans and 90.2% for T. halophilus. The quantification limits of the assays were 10(3) CFU/mL and 10(2) CFU/mL in fish sauce samples with linear correlations over 4 Logs for V. halodenitrificans and T. halophilus, respectively. The matrix effect was not observed when evaluated using fish sauce samples fermented for 1-6 months. The developed PMA-qPCR methods were successfully applied to monitor changes of Virgibacillus sp. SK37 and T. halophilus MS33 in a mackerel fish sauce fermentation model where culture-dependent techniques failed to quantify the starter cultures. The results demonstrated the usability of the methods as practical tools for monitoring the starter cultures in fish sauce fermentation.
- Published
- 2016
44. Chemical Parameters for Traceability of Raw Material Freshness of Tropical Surimi
- Author
-
Wasana Wongngam, Buratin Khampirat, and Jirawat Yongsawatdigul
- Subjects
biology ,Nemipterus ,010401 analytical chemistry ,Trimethylamine ,04 agricultural and veterinary sciences ,Aquatic Science ,Raw material ,Goatfish ,biology.organism_classification ,040401 food science ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,Upeneus ,0404 agricultural biotechnology ,chemistry ,Total volatile ,Saurida ,Threadfin bream ,Food science ,Food Science - Abstract
Changes in chemical parameters of tropical fish used for surimi production—namely, threadfin bream (TB; Nemipterus spp.), lizardfish (LZ; Saurida spp.), and goatfish (GF; Upeneus spp.)—during ice storage and their respective washed mince were investigated. Total volatile basic nitrogen (TVB-N), trimethylamine (TMA), hypoxanthine (Hx), and total biogenic amine (BA) content of mince increased with storage time. Crude protein of fish and their respective washed mince reduced as storage time of fish was prolonged. Principal component analysis (PCA) revealed that washed mince produced from fish stored in ice for 7–12 days showed chemical characteristics that were distinct from those of washed mince prepared from spoiled fish. The TMA, TVB-N, and Hx content of washed mince appeared to be sufficient raw material freshness indicators in these three species.
- Published
- 2016
45. Chemical and Cellular Antioxidant Activities of In Vitro Digesta of Tilapia Protein and Its Hydrolysates
- Author
-
Jirawat Yongsawatdigul, Parinya Noisa, and Xiaogang Zhang
- Subjects
animal structures ,Health (social science) ,Antioxidant ,Oxygen radical absorbance capacity ,030309 nutrition & dietetics ,medicine.medical_treatment ,Trolox equivalent antioxidant capacity ,antioxidant activity ,Plant Science ,lcsh:Chemical technology ,Health Professions (miscellaneous) ,Microbiology ,Article ,Hydrolysate ,03 medical and health sciences ,Hydrolysis ,0404 agricultural biotechnology ,Pepsin ,medicine ,oxidative stress ,lcsh:TP1-1185 ,Food science ,chemistry.chemical_classification ,0303 health sciences ,biology ,04 agricultural and veterinary sciences ,040401 food science ,Enzyme ,chemistry ,biology.protein ,in vitro GI digestion ,Digestion ,protein hydrolysate ,Food Science - Abstract
Production of protein hydrolysate as nutraceuticals is typically based on the activity of the hydrolysate, which might not yield the optimal activity under physiological condition due to structural modification of peptides upon gastrointestinal (GI) digestion. This study systematically compared the chemical and cellular antioxidant activities of the in vitro digesta of tilapia protein and its hydrolysates prepared with various degree of hydrolysis (DH) by Alcalase. The enzymes used in the in vitro GI digestion analysis significantly contributed to the peptide content, Trolox equivalent antioxidant capacity (TEAC), and oxygen radical absorbance capacity (ORAC). Proteins and all hydrolysates were slightly digested by pepsin but hydrolyzed extensively by pancreatin. Both hydrolysate and digesta predominantly scavenged free radicals via hydrogen atom transfer (HAT). The antioxidant activities of the hydrolysates increased with the increasing DH up to 16 h of hydrolysis. However, the digesta of 10-h hydrolysate displayed the highest chemical and HepG2 cellular antioxidant activities, while the protein digesta displayed the lowest. Principal component analysis (PCA) showed that the TEAC of the digesta was positively correlated with the cellular antioxidant activity (CAA). Therefore, the production of protein hydrolysate should be optimized based on the activity of the hydrolysate digesta rather than that of hydrolysates.
- Published
- 2020
46. Characterization of the antioxidant and ACE-inhibitory activities of Thai fish sauce at different stages of fermentation
- Author
-
Jirawat Yongsawatdigul, Ali Hamzeh, and Parinya Noisa
- Subjects
0301 basic medicine ,Antioxidant ,Fish sauce ,medicine.medical_treatment ,Medicine (miscellaneous) ,Bioactive peptide ,03 medical and health sciences ,symbols.namesake ,0404 agricultural biotechnology ,Downregulation and upregulation ,ACE inhibitor ,medicine ,TX341-641 ,Food science ,Gene ,chemistry.chemical_classification ,030109 nutrition & dietetics ,Nutrition and Dietetics ,Nutrition. Foods and food supply ,04 agricultural and veterinary sciences ,040401 food science ,Cellular antioxidant ,Amino acid ,Maillard reaction ,Enzyme ,chemistry ,symbols ,Fermentation ,Leucine ,Food Science - Abstract
Six-month-old (FS6) and 12-month-old (FS12) Thai fish sauces were separated by Sephadex-G25 desalting columns into 3 fractions, namely, F1 (peptides/Maillard reaction products), F2 (peptides/NaCl) and F3 (peptides/amino acids). The F3 fractions from both samples showed the highest chemical and cellular antioxidant activities and could inhibit ACE (P
- Published
- 2020
47. Improvement of Fish Sauce Quality by Strain CMC5-3-1: A Novel Species ofStaphylococcussp
- Author
-
Somboon Tanasupawat, Natteewan Udomsil, Jirawat Yongsawatdigul, and Sureelak Rodtong
- Subjects
Hydrolyzed protein ,biology ,Umami ,biology.organism_classification ,Fish products ,medicine.disease_cause ,16S ribosomal RNA ,Tryptic soy broth ,Microbiology ,chemistry.chemical_compound ,chemistry ,medicine ,Staphylococcus piscifermentans ,Staphylococcus ,Food Science ,Fermented fish - Abstract
Staphylococcus sp. CMC5-3-1 and CMS5-7-5 isolated from fermented fish sauce at 3 to 7 mo, respectively, showed different characteristics on protein hydrolysis and volatile formation. These Gram-positive cocci were able to grow in up to 15% NaCl with the optimum at 0.5% to 5% NaCl in tryptic soy broth. Based on ribosomal 16S rRNA gene sequences, Staphylococcus sp. CMC5-3-1 and CMS5-7-5 showed 99.0% similarity to that of Staphylococcus piscifermentans JCM 6057(T) , but DNA-DNA relatedness was
- Published
- 2015
48. Characterization of bacteriocin produced by Enterococcus faecium CN-25 isolated from traditionally Thai fermented fish roe
- Author
-
Michael L. Chikindas, Sureelak Rodtong, Jirawat Yongsawatdigul, and Noojaree Sonsa-Ard
- Subjects
biology ,Bran ,food and beverages ,biology.organism_classification ,medicine.disease_cause ,Bacteriocin ,Biochemistry ,Listeria monocytogenes ,ATP hydrolysis ,medicine ,Efflux ,Bacteria ,Food Science ,Biotechnology ,Fermented fish ,Enterococcus faecium - Abstract
Enterococcus faecium CN-25 was isolated from a traditionally Thai fermented fish roe, som-kai-pla, and was found to possess enterocin A and B genes. Among the 30 microorganisms tested, the produced bacteriocin exhibited antagonistic activity against Listeria monocytogenes TISTR 1327. The bacterium maximally produced bacteriocin at a concentration of 1828 AU/ml at the beginning of the stationary phase (at 18 h) in the inexpensive rice bran medium. The antilisterial activity of the compound was not affected by heating at 60–100 °C for up to 30 min. High antilisterial activity remained at pH 2. Partially purified CN-25 bacteriocin derived from E. faecium CN-25 completely inhibited the growth of L. monocytogenes at the minimum concentration of 2.38 μg/ml. The compound had no effect on the efflux of ATP from L. monocytogenes but triggered the intracellular hydrolysis of ATP, as evidenced by a decrease in the luminescence of total ATP. The bacteriocin also depleted the transmembrane electrical potential (ΔΨ) but had no effect on the transmembrane pH gradient (ΔpH). Therefore, the partially purified CN-25 peptide may be useful for improving the product safety of fermented fish products.
- Published
- 2015
49. Characterization and identification of angiotensin I-converting enzyme (ACE) inhibitory peptides derived from tilapia using Virgibacillus halodenitrificans SK1-3-7 proteinases
- Author
-
Sittiruk Roytrakul, Tidarat Toopcham, and Jirawat Yongsawatdigul
- Subjects
chemistry.chemical_classification ,Nutrition and Dietetics ,Chromatography ,ACE inhibitory peptide ,Nutrition. Foods and food supply ,Size-exclusion chromatography ,Medicine (miscellaneous) ,Peptide ,Protein hydrolysate ,Virgibacillus halodenitrificans SK1-3-7 ,Hydrolysate ,Bioactive peptide ,Hydrolysis ,Ultrafiltration (renal) ,Enzyme ,chemistry ,Biochemistry ,TX341-641 ,Uncompetitive inhibitor ,Food Science ,Thermostability ,Tilapia - Abstract
Angiotensin I-converting enzyme (ACE) inhibitory activity of tilapia mince (TM) hydrolyzed by Virgibacillus halodenitrificans SK1-3-7 proteinases with a 48% degree of hydrolysis showed the highest ACE inhibitory activity with an IC 50 of 0.54 mg/mL. After ultrafiltration (UF) and chromatographic separation via anion exchange, cation exchange and size exclusion chromatography, the fraction with the highest ACE inhibitory activity with an IC 50 of 0.15 mg/mL was obtained. The peptides showed uncompetitive inhibition characteristics with an inhibition constant (K i ) of 0.18 mg/mL. The peptides showed high thermostability at 100 and 121 °C, as well as pH stability at a wide pH range of 2–10, and also maintained ACE inhibitory activity after in vitro gastrointestinal digestion. The most potent novel ACE inhibitory peptide identified was MILLLFR with an IC 50 of 0.12 µM. TM hydrolysate prepared by V. halodenitrificans SK1-3-7 proteinases could serve as a source of peptides with ACE inhibitory activity.
- Published
- 2015
50. Chemical and cellular antioxidative properties of threadfin bream (Nemipterus spp.) surimi byproduct hydrolysates fractionated by ultrafiltration
- Author
-
Hang Xiao, Chompoonuch Wiriyaphan, Eric A. Decker, and Jirawat Yongsawatdigul
- Subjects
Antioxidant ,Protein Hydrolysates ,medicine.medical_treatment ,Ultrafiltration ,Antioxidants ,Hydrolysate ,Analytical Chemistry ,chemistry.chemical_compound ,Lactate dehydrogenase ,Endopeptidases ,medicine ,Animals ,Humans ,Cytotoxicity ,biology ,Nemipterus ,Fishes ,General Medicine ,biology.organism_classification ,In vitro ,Perciformes ,chemistry ,Biochemistry ,Threadfin bream ,Reactive Oxygen Species ,Intracellular ,Food Science - Abstract
Protein hydrolysate from frame, bone and skin (FBSH) of threadfin bream was prepared using Virgibacillus sp. SK33 proteinase and fractionated using sequential ultrafiltration membranes with molecular weight cut-offs (MWCO) of 30, 5 and 1 kDa, respectively. Four fractions, namely FBSH-I (>30 kDa), FBSH-II (5-30 kDa), FBSH-III (1-5 kDa), and FBSH-IV (
- Published
- 2015
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