1. Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes
- Author
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Wang Haidong, Li Jingwei, Bai Yun, Wang Juan, Li Hongquan, Luo Xiaomao, Dong YanJun, Li Lixin, Yu XiuJu, Zhu ZhiWei, and He Xiaoyan
- Subjects
0301 basic medicine ,Histology ,Blotting, Western ,Myostatin ,Immunofluorescence ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Western blot ,medicine ,Myocyte ,Animals ,Muscle, Skeletal ,Cells, Cultured ,biology ,medicine.diagnostic_test ,NFATC Transcription Factors ,Chemistry ,Calcineurin ,Cell Biology ,General Medicine ,Cobalt ,Hypoxia (medical) ,musculoskeletal system ,In vitro ,Cell Hypoxia ,Cell biology ,030104 developmental biology ,030220 oncology & carcinogenesis ,biology.protein ,Signal transduction ,medicine.symptom ,Transcriptome ,C2C12 ,Signal Transduction - Abstract
This study investigated the effects of a CoCl2-simulated hypoxic environment on the muscle fiber switching signaling pathways calcineurin A/nuclear factor of activated T cells cytoplasmic 1 (CnA/NFATc1) and myostatin. In this study, C2C12 muscle cells were cultured in vitro under CoCl2-simulated chemical hypoxic conditions, the expression levels of CnA and myostatin were detected through qRT-PCR and Western blot analyses, and a positioning study of NFATc1 was carried out by immunofluorescence labeling. Results showed that CoCl2 treatment significantly increased the expression levels of CnA and myostatin. Moreover, the position of NFATc1 expression changed; actually, its expression in the nucleus considerably increased. Furthermore, CoCl2-induced hypoxia inhibited the differentiation of C2C12 cells and reduced the expression levels of many slow- and fast-twitch muscles marker genes, but immunofluorescence staining results showed that the proportion of MyHC I type muscle fiber increased after CoCl2 treatment. The hypoxic environment simulated by CoCl2 can activate the signaling pathways CnA/NFATc1 and myostatin and increases the proportion of MyHC I type muscle fibers.
- Published
- 2018