1. Results of a collaborative study on DNA identification of aged bone samples
- Author
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Judith Zander, Marie Allen, Joanna Vella, Petra Lotz, Ruth Galdies, Alain Stevanovitch, Olivier Cepil, Marion Nagy, Daniel Vanek, Wafa Ali Rashid Al Tayyari, Dina Al Salafi, Hicham El Ossmani, Mishel Stephenson, Jindrich Novotny, Jan Cemper-Kiesslich, Jan Frolík, Jitka Dubska-Votrubova, Greet De Cock, Michel Bottinelli, Francois Guidet, Marguerethe Stenersen, Juliane Strien, Wendy Arguetaa, Magdalena M. Bus, Angie Ambers, Anna Hoefges, Cristian Bogdan Iancu, D.R. Sumita, Ahmed Anwar Al Afeefi, Alessandro Maresca, Hajar Rachid, Jessica Rothe, Bruce Budowle, Martin Pospíšek, Stijn Desmyter, Hamid El Amri, Khudooma Saeed Al Naimi, Khalid Ismaeil Al Hosani, and Sebastian Grün
- Subjects
Forensic Genetics ,0301 basic medicine ,medicine.medical_specialty ,Forensic Science ,Bone and Bones ,03 medical and health sciences ,Forensic dna ,0302 clinical medicine ,medicine ,Humans ,030216 legal & forensic medicine ,Czech Republic ,business.industry ,National museum ,Medical jurisprudence ,DNA ,General Medicine ,DNA Fingerprinting ,Dna identification ,030104 developmental biology ,Multicenter study ,DNA profiling ,Family medicine ,Test performance ,Degraded dna ,business - Abstract
Aim A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. Methods Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. Results Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. Conclusion The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.
- Published
- 2017