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This study aims to explore the temperature field and mechanical characteristics of hydraulic tunnels during operation in an area with large temperature differences throughout a year. By relying on the measured displacement field change results of a water transmission tunnel in Xinjiang, a finite element calculation model applicable to the hydraulic tunnel-surrounding rock under the action of large temperature differences was established. Then, the FEM model was used to analyze the temperature field distribution and stress field variation of hydraulic tunnel-surrounding rock under the effect of large temperature differences. The results showed that, under the action of large temperature differences, the displacement of the cave top was the largest, followed by the cave bottom, and finally the cave waist. The obtained displacement field data using the calculation model in this study were basically consistent with the on-site measured data, and the displacement field change law was the same, indicating that the established finite element calculation model is reliable. When the ambient temperature was below zero, the freezing front gradually expanded to the depth of the surrounding rock along the tunnel radial direction, while the frozen rock at the intersection of the lining and the surrounding rock melted first when the ambient temperature was above zero. The stress in various parts of the surrounding rock of the hydraulic tunnel increased continuously. To be specific, the growth rate of the tensile stress at the cave bottom, the tensile stress at the cave top, and the compressive stress at the cave waist of the tunnel reached 0.052 MPa/year, 0.053 MPa/year, and 0.096 MPa/year, respectively, in the first year, but the increasing rate subsequently tended to slow down continuously. Therefore, in actual projects, attention should be focused on the effect of large temperature differences on the stress growth of tunnel-surrounding rocks at an early stage, especially the growth of compressive stress at the cave waist. Notably, there are a large number of symmetrical and asymmetric phenomena related to engineering in this study.

Aim To determine the tolerance and acceptance of hepatic venous pressure gradient (HVPG) measurements in patients with liver cirrhosis. Methods This prospective international multicenter study included 271 patients with cirrhosis who were scheduled to undergo HVPG measurement between October 2019 and June 2020. Data related to the tolerance and acceptance of HVPG measurements were collected using descriptive questionnaires. Results HVPG measurements were technically successful in all 271 patients, with 141 (52.0%) undergoing HVPG measurement alone. The complication rate was 0.4%. Postoperative pain was significantly lower than preoperative expected pain (p

High-belite cement (HBC) is increasingly used in hydraulic concrete, of which the frost resistance is an important index in cold climatic areas. In the present study, the mass loss, dynamic modulus, cube compressive strength, micro-morphology and pore characteristic parameters of HBC concrete and reference specimens were investigated under freezing and thawing, and their internal frost damages were quantified by micro-Vickers hardness and ultrasonic theory. Test results showed that the frost resistance of HBC concrete was similar to that of ordinary Portland cement (OPC) concrete under the same proportioning, and that their freeze–thaw failure was a continuous and asynchronous process of both surface-to-interior damage and overall deterioration caused by the test mechanism and specimen size. Service life of concrete could be calculated through the thickness of the damaged layer obtained by ultrasonic theory, and models of Vickers hardness distribution and compressive strength prediction based on equivalent Vickers hardness were proposed to evaluate the performance of frozen concrete with high accuracy. This potentially provides a better understanding of freeze–thaw damage and a reference for the popularisation and application of HBC concrete.

In this study, the mechanical behavior of fully grouted rock bolts in hydraulic tunnels subjected to elevated ground temperatures was investigated. A differential equation for axial displacement of the rock bolt was formulated, which considers the force equilibrium of infinitesimal bolt segments and the stress transfer mechanism at the anchor–rock interface. The distribution functions for axial stress within the bolt and the interfacial shear stress were obtained by solving the differential equation, which incorporated the displacement of the surrounding rock mass as a parameter. This study showed that the effectiveness of the bolt–shotcrete support system decreases over time, considering the displacement relaxation rate of the surrounding rock mass. The mechanical model’s variation laws at 20 °C, 50 °C, and 80 °C were summarized by integrating the thermal deformation equation for material parameters, and the numerical simulation results were compared and analyzed. The findings revealed that the bond strength between the rock bolt and the rock mass diminishes as the temperature of the surrounding rock increases, leading to a reduction of interfacial shear stress at both extremities of the bolt. Moreover, the maximum axial force within the bolt escalates as the neutral point migrates farther from the tunnel wall.

Supplementary Tables S1-S6 and Supplementary Figures S1-S2. Table S1: Data collection and refinement statistics, related to Figure 1B; Table S2: Summary of cell line related information; Table S3: BGB-283 is a reversible B-RAF inhibitor; Table S4. Kinase inhibition profile of BGB-283. BGB-283 selectively inhibited multiple kinases at concentration of 10 μM; Table S5: IC50 values of BGB-283 in inhibiting various kinases; Table S6. Anti-tumor efficacy of BGB-283 in CRC xenograft models; Figure S1: BGB-283 is a slow-off B-RAF inhibitor showing potent anti-EGFR activity in multiple cell lines; Figure S2. The effect of BGB-283 on body weight of nude mice subcutaneously implanted with human cancer xenografts.

Objective To summarize and think about the surgical process of Da Vinci Xi™ robot-assisted liver resection. Methods To summarize the clinical data, preoperative preparations, surgical procedures, and postoperative recovery of a patient who underwent Da Vinci Xi™ robot-assisted left hepatectomy in the third ward of the Department of Hepatobiliary and Pancreatic Surgery of the Second Affiliated Hospital of Kunming Medical University in 2021. Discussion to guide future clinical work. Results The left hemihepatectomy was performed safely and effectively on patients with left intrahepatic bile duct stones with the assistance of Da Vinci Xi™ robot. Conclusion Da Vinci Xi™ robot's three-dimensional field of vision, stable and flexible manipulator operation and other advantages make it suitable for liver resection surgery.

The reliability and stability of the seal at the fuel-supply end of a rocket-engine turbopump are important factors in determining safety. Conventional single-stage floating rings used for the isolation-sealing of cryogenic media are highly susceptible to operational instability during startup and shutdown, which places demands on the seals’ structure, size, and material properties. In this study, a two-stage floating-ring isolation seal with a non-slotted main sealing surface was designed using a tangential air-intake mode. Based on the full-size three-dimensional finite-volume model, the leakage characteristics of the floating ring during operation were calculated, taking into account the effect of the “inlet effect” on the seal’s performance. Combining the temperature and pressure distributions of the sealing system under cryogenic operating conditions, calculated using a numerical simulation, a reliability analysis of various inlet directions and two kinds of floating-ring schemes was carried out on a self-constructed service-performance test bench. The results indicate that the main wear location of the non-slotted floating ring occurs on the auxiliary sealing surface, with stable working performance. When the inlet direction and spindle-rotation direction are the same, this is more conducive to ensuring the stability of seal performance in practical applications. The results of the current research are instructive for designing floating-ring isolation seals for turbine pumps.

A spring-energized seal, whose PTFE plastic shell has excellent self-lubrication and a low temperature stability, is used widely in liquid fuel valves’ rotating end-face seals. However, in practical application, temperature has a larger effect on not only the physical and tribological properties of materials, but also on the leakage performance of spring-energized rings. Thus, a high and low temperature sealing test of the spring-energized seal that applies to an engine was carried out. In this paper, the leakage characteristics, friction torque and wear characteristics of a spring-energized ring under different temperatures were studied. The friction torque at high temperature was less than that at normal temperature, and a low temperature could effectively reduce the wear amount of PTFE material. In order to study the influence of temperature on PTFE filled with graphite, the friction and wear test of PTFE-2 was carried out. The results showed that the amount of wear of PTFE-2 was only 27.8% of that at the normal temperature but the friction coefficient was three times larger when the temperature was −45 °C. Godkänd;2023;Nivå 0;2023-03-13 (joosat);Konferensartikel i tidskriftPart of Special Issue: Selected Papers from CITC2022Licens fulltext: CC BY License

The physicochemical property, volatile flavor compounds, and microbial community structure of Jinhua fatty ham (FH) and lean ham (LH) were investigated and compared by high-throughput sequencing and HS-GC-IMS. Results showed that FH had higher pH and slightly lighter and yellower color than LH. Meanwhile, 33 volatile flavor compounds were identified from FH and LH, among which LH showed higher abundance of total alcohols and acids, but FH had generally richer aldehydes, ketones, esters, heterocyclic, and sulfur-containing compounds. Moreover, FH and LH did not have significant difference in α-diversity of bacterial community, but LH presented a much lower α-diversity of fungal community than FH. Besides, the dominant microorganisms (relative abundance >2%) in FH were Ruminococcaceae UCG-005, Staphylococcus, Ruminococcaceae UCG-014, Meyerozyma, and Aspergillus at the genus level, while in LH were Staphylococcus, Psychrobacter, Halomonas, Propionicicella, Ruminococcaceae UCG-005, Meyerozyma, Yamadazyma, and Aspergillus. Furthermore, the analysis of Pearson’s correlation and metabolic network confirmed that the discriminative flavor compounds of FH were mainly β-oxidation and degradation products of fatty acids, while those of LH were mostly derived from the Strecker reaction or microbial metabolism of amino acids. The present study could help understand the potential pathway of characteristic microorganisms affecting flavor formation of fat-deficient dry-cured hams and provide theoretical supports for developing healthier fermented meat products.

Workplace accommodations can reduce barriers to employment for people who use augmentative and alternative communication (AAC), however, the lack of accommodations continues to challenge participation in employment. This systematic review identified and analyzed barriers and facilitators to implementing workplace accommodations for adults (19 years and over) who use AAC. A systematic search of nine databases was conducted to identify relevant studies using the search terms “AAC” and “workplace accommodations” and variations of each term. Results were imported into Covidence. Seventeen studies met the inclusion criteria. Results were presented using the International Classification of Functioning, Disability, and Health (ICF) framework. The Oxford levels of evidence and Confidence in Evidence from Review of Qualitative Research (GRADE-CERQual) were used to assess the quality of the studies and confidence in findings, respectively. Environmental barriers related mainly to attitudes and technology, and personal barriers related to job qualifications, education, and work-related skills. A combination of facilitators such as personal strengths, access to technology, and supportive relationships contributed to successful implementation of accommodations. The findings of this review suggest that implementing workplace accommodations for adults who use AAC strategies is complex and further research is needed to advance practices and policies that support the implementation of workplace accommodations.

Magnetorheological fluid seal and brush seal are successful sealing forms. To address problems such as pole pieces colliding with spindles, precise sealing clearance in magnetic fluid seals, and inevitable leakage in brush seals, this article combines the advantages of magnetic fluid seal and brush seal and proposes a magnetorheological fluid seal with flexible pole pieces. The magnetic field of the seal was estimated using the finite-element method and the seal capacity was studied in theory. Single structural parameters of the seal that affect sealing capacity such as the relative permeability and thickness were discussed. The combined effects of various structural parameters were analyzed. The results show that an increase in the relative permeability and length of the flexible pole pieces leads to the enhancement of the seal capacity. The thickness of the flexible magnetic pole piece and the magnetic part of the flap and coating are inversely proportional to the sealing pressure value. The experimentally obtained seal capacity agrees with the value calculated through the simulation. This article presents a structure of the magnetorheological fluid seal and comparative results of the seal capacity obtained from both experimental studies and numerical simulation calculations.

Amyloid precursor protein (APP) metabolism is central to Alzheimer’s disease (AD) pathogenesis, but the key etiological driver remains elusive. Recent failures of clinical trials targeting amyloid-β (Aβ) peptides, the proteolytic fragments of amyloid precursor protein (APP) that are the main component of amyloid plaques, suggest that the proteostasis-disrupting, key pathogenic species remain to be identified. Previous studies suggest that APP C-terminal fragment (APP.C99) can cause disease in an Aβ-independent manner. The mechanism of APP.C99 pathogenesis is incompletely understood. We used Drosophila models expressing APP.C99 with the native ER-targeting signal of human APP, expressing full-length human APP only, or co-expressing full-length human APP and β-secretase (BACE), to investigate mechanisms of APP.C99 pathogenesis. Key findings are validated in mammalian cell culture models, mouse 5xFAD model, and postmortem AD patient brain materials. We find that ribosomes stall at the ER membrane during co-translational translocation of APP.C99, activating ribosome-associated quality control (RQC) to resolve ribosome collision and stalled translation. Stalled APP.C99 species with C-terminal extensions (CAT-tails) resulting from inadequate RQC are prone to aggregation, causing endolysosomal and autophagy defects and seeding the aggregation of amyloid β peptides, the main component of amyloid plaques. Genetically removing stalled and CAT-tailed APP.C99 rescued proteostasis failure, endolysosomal/autophagy dysfunction, neuromuscular degeneration, and cognitive deficits in AD models. Our finding of RQC factor deposition at the core of amyloid plaques from AD brains further supports the central role of defective RQC of ribosome collision and stalled translation in AD pathogenesis. These findings demonstrate that amyloid plaque formation is the consequence and manifestation of a deeper level proteostasis failure caused by inadequate RQC of translational stalling and the resultant aberrantly modified APP.C99 species, previously unrecognized etiological drivers of AD and newly discovered therapeutic targets. Supplementary Information The online version contains supplementary material available at 10.1186/s40478-021-01268-6.

The current fertilizer recommendations for melon plantation have many limitations and exhibit deficiencies regarding future development. Therefore, in this study, the optimal quantities of fertilizer, in terms of the effects of single factors and interaction effects, are studied. There were significant interaction effects between N and P, N and K, P and K; the contents of soluble protein, vitamin C (Vc), and soluble sugar in melon could be improved using the optimal fertilization ratios. The optimal ratio of N:P:K was 2.33:1:3.85, with the amounts of N, P2O5, and K2O, respectively, being 157.5, 67.58, and 260.38 kg/hm2, yielding 8.73 g/kg of soluble protein in melon. The optimal ratio of N:P:K was 2.03:1:3.36, with amounts of N, P2O5, and K2O being 157.50, 77.40, and 260.38 kg/hm2, respectively, yielding 25.32 g/kg Vc content in melon. Finally, the optimal ratio of N:P:K was 1.53:1:3.36, with the amounts of N, P2O5, and K2O being 118.07, 77.40, respectively, and 260.38 kg/hm2, yielding 13.34% soluble sugar content in melon.

Background The safety and short-term outcomes of gastrectomy after preoperative chemotherapy plus immunotherapy (PCIT) versus preoperative chemotherapy (PCT) in patients with advanced gastric cancer (AGC) remain unclear. This study was conducted to compare the safety and short-term efficacy of PCIT with those of PCT in patients with AGC. Methods We retrospectively reviewed the data of patients with AGC who received PCIT or PCT at Peking University Cancer Hospital and Institute Gastrointestinal Cancer Center I between January 2019 and June 2021. The clinical characteristics were recorded, and short-term oncological outcomes were compared. Independent t tests, Mann‒Whitney U tests, chi-square tests, and Fisher’s exact tests were used to calculate differences. The correlation analyses were performed using Pearson correlation. All p values were two-sided, and a p value Results A total of 162 AGC patients were included in this study, including 25 patients who received PCIT and 137 patients who received PCT. There were no significant differences in preoperative treatment-related adverse events (TRAEs) between the PCIT group and the PCT group (p = 0.088). Compared with the PCT group, the PCIT group had comparable postoperative functional recovery, with no significant differences in terms of time to first aerofluxus (p = 0.349), time to first defecation (p = 0.800), time to liquid diet (p = 0.233), or length of stay (p = 0.278). No significant differences were observed in terms of postoperative complications (p = 0.952), postoperative pain intensity at 24, 48, or 72 h (p = 0.375, p = 0.601, and p = 0.821, respectively), or postoperative supplementary analgesic use between the two groups (p = 0.881). In addition, the postoperative complication rate was 33.3% following laparoscopic approaches and 31.2% following open approaches in the PCIT group, with no significant difference (p = 1.000). Conclusion In patients with AGC, gastrectomy with D2 or D2 + lymphadenectomy after PCIT had comparable short-term oncological outcomes to PCT.

The Dendrobium officinale flower is a non-medicinal part of the plant, rich in a variety of nutrients and bioactive ingredients. The purpose of this article was to explore the preparation conditions of anthocyanins (ACNs) from the D. officinale flower. Subsequently, its anti-aging effects were evaluated with Caenorhabditis elegans. Results showed that the ACNs had antioxidant activities on scavenging free radicals (DPPH· and ABTS+·), and the clearance rate was positively correlated with the dose. Additionally, ACNs significantly increased the activity of superoxide dismutase (SOD) in C. elegans, which was 2.068-fold higher than that of the control. Treatment with ACNs at 150 μL extended the lifespan of C. elegans by 56.25%, and treatment with ACNs at 50 μL promoted fecundity in C. elegans. Finally, the protective effect of ACNs enhanced stress resistance, thereby increasing the survival numbers of C. elegans, which provided insights for the development and practical application of functional products.

Graphite-based composites are well recognized as ideal functional materials in mechanical seals, bearings of canned pumps, and electrical contact systems because of their outstanding self-lubricating ability, thermostability, and chemical stability. Working in harsh conditions is a huge challenge for the graphite materials, and their tribological properties and wear mechanisms are not well studied. In this study, the tribological performance of metal-impregnated graphite, resin-impregnated graphite, and non-metal-impregnated graphite under high temperature and high load are studied using a ball-on-disc tribometer. The results show that the metal-impregnated graphite (Metal-IG) has a stable friction regime and exhibits better anti-friction and anti-wear properties than that of resin-impregnated graphite (Resin-IG) and non-impregnated graphite (Non-IG) under extreme pressure (200~350 MPa) and high temperature (100–350 °C). The Metal-IG and Resin-IG can reduce the wear depth by 60% and 80%, respectively, when compared with Non-IG substrate. The impregnated materials (metal or resin) can enhance the strength of the graphite matrix and improve the formation of graphite tribofilm on the counterpart surfaces. Friction-induced structural ordering of graphite and slight oxidation of metal in the formed mechanically mixed layer is also beneficial for friction and wear reduction. This study demonstrates the tribological characteristics of impregnated graphite under harsh conditions and provides the experimental basis for the advanced usage of high-reliability and self-lubrication graphite composites.

The

Soil ammonia oxidation, which acts as the first and rate-limiting step of nitrification, is driven by ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA) and complete ammonia oxidizer (comammox, amoA gene of clade-A and clade-B). Straw returning, widely used ecological technology in China, is an effective measure for promoting straw decomposition and soil nutrient cycling when combined with earthworm addition. However, the effects of straw returning combined with earthworm addition on soil ammonia oxidizers remain poorly understood.A 2-year plot experiment was conducted with 5 treatments: no fertilizer (CK); regular fertilization (RT); straw returning (SR); earthworm addition (W); straw returning + earthworm addition (SRW). The AOA, AOB, comammox clade-A and clade-B community microbial diversities and structures were investigated by high-throughput sequencing.The results showed that (1) compared to RT treatment, W, SR, and SRW treatments all significantly increased the richness of AOA and comammox clade-A and clade-B (This study provides scientific support for the application of straw returning combined with earthworm addition to improve soil nitrification with respect to soil ammonia-oxidizing microorganisms.

Neoadjuvant chemotherapy (NACT) before radical gastrectomy is preferred for locally advanced gastric cancer (GC). However, clinical practices demonstrate that a considerable proportion of GC patients do not benefit from NACT, largely due to the lack of biomarkers for patient selection and prognosis prediction. A recent study revealed that patients with microsatellite instability-high (MSI-H) may be resistant to NACT, however, most tumors in Chinese GC patients (~ 95%) are characterized by microsatellite stability (MSS). Here, we aimed to discover new molecular biomarkers for this larger population. We performed whole-exome sequencing on 46 clinical samples (pre- and post-treatment) from 30 stage II/III MSS GC patients whose response to NACT was rigorously defined. Serum tumor markers (TMs), including AFP, CEA, CA199, CA724 and CA242 were measured during the course. High tumor mutation burden (TMB-H) and 19q12 amplification (19q12 +) were positively associated with the NACT response. When TMB and 19q12 amplification were jointly analyzed, those with TMB-H or 19q12 + showed favorable response to NACT (p = 0.035). Further, TMB-H was negatively correlated with ypN stage, lymph node metastasis, and macrophage infiltration. Patients with TMB-H showed better disease-free survival (DFS) than those with TMB-L (P = 0.025, HR = 0.1331), and this was further validated using two larger GC datasets: TCGA-STAD (p = 0.004) and ICGC-CN (p = 0.045). The combination of TMB-H and 19q12 + can serve as an early indicator of response to NACT. Superior to traditional clinical indicators, TMB-H is a robust and easily accessible candidate biomarker associated with better DFS, and can be evaluated at the time of diagnosis.

Neoadjuvant chemotherapy (NACT) and laparoscopic surgery have been increasingly used in the treatment of gastric cancer, however, the feasibility and safety of totally laparoscopic gastrectomy after NACT still remain unknown.At the Gastrointestinal cancer center of Peking university cancer hospital and institute in Beijing, clinical and pathological data of patients who has received NACT, followed by radical laparoscopic gastrectomy was retrospectively reviewed between March 2011 and November 2019. Patients were divided into 2 groups according to whether intracorporeal anastomosis or extracorporeal anastomosis had been performed, short-term outcomes (post-operative recovery index and complications) and economic cost were compared between 2 groups.All of 139 patients underwent laparoscopic gastrectomy. 87 [62.6%] patients had totally laparoscopic gastrectomy (TLG) and 52 [37.4%] patients had laparoscopic-assisted gastrectomy (LAG). Overall complication rate was 28.8% in all patients. TLG group was significantly associated with lower overall complication rate (21.8% VS 40.4%; p = 0.019) and major complication rate (3.4% VS 13.5%; p = 0.001) compared with LAG group. Overall cost was similar (p = 0.077). In subgroup analysis, totally laparoscopic total gastrectomy (TLTG) group showed lower overall postoperative complication rate (19.0% VS 56.5%; p = 0.011), as well as marginal significant differences in major complication (0% VS 21.7%; p = 0.05) than laparoscopic-assisted total gastrectomy (LATG) group. Earlier first liquid diet (4 [3.5-5] day VS 6 [4-6.5] day; p = 0.047), earlier first aerofluxus (3 [3-4] day VS 4 [3-4.5] day; p = 0.02) and a shorter hospital stay (9 [8-12] day VS 12 [10-15] day; p = 0.004) were observed in TLTG group. Overall and major complication rate were similar in totally laparoscopic distal gastrectomy (TLDG) and laparoscopic assisted distal gastrectomy (LADG) group (22.7% VS 27.6%; p = 0.611; 4.5% VS 6.9%; p = 0.639; respectively). Significant differences were found between TLDG and LADG groups regarding time to first liquid diet (4 [3-5] day VS 6 [3.75-6] day; p = 0.006), time to first aerofluxus (3 [3-3] day VS 4 [3-6] day; p 0.001), time to first defecation (4 [4-5] day VS 5 [4-6] day; p = 0.045), time to remove all drainage (7 [6-8] day VS 8 [6-9] day; p = 0.021), white blood cell count on postoperative Day 1 (9.54 ± 2.49 10For patients with Locally advanced gastric cancer who received NACT, totally laparoscopic gastrectomy, including TLTG and TLDG, doesn't increase complications and overall cost compared with LAG, and has advantages in gastrointestinal function recovery, incision length and postoperative hospital stay.

Background The prognostic values of preoperative tumor markers (TMs) remain elusive in patients with locally advanced gastric cancer (LAGC) after neoadjuvant chemotherapy treatment (NACT). This study aimed to assess and establish a novel scoring system incorporating carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), carbohydrate antigen 72-4 (CA72-4) to enhance prognostic accuracy for progression-free survival (PFS) and pathological response (pCR). Methods Patients' data were retrospectively analyzed from December 2006 to December 2017 in our center. The cutoff value of TMs was determined using the time-dependent receiver operating test characteristics method. These three TMs were allocated 1 point each for the post neoadjuvant chemotherapy combination of tumor markers (post-NACT CTM) scores. The training group comprised 533 patients, responsible for full analysis, and the validation group comprised 137 patients based on the selection protocol. Results Of 533 enrolled patients, 138, 233, 117, and 45 patients scored 0, 1, 2, 3 respectively. The 3-year PFS rate Multivariate analysis revealed that post-NACT CTM score was an independent predictor of PFS (0 vs. 1, HR: 1.34, 95% CI: 0.92–1.96, P = 0.128; 0 vs. 2, HR: 2.03, 95% CI: 1.35–3.05, P = 0.001; 0 vs. 3, HR: 2.98, 95% CI: 1.83–4.86, P P = 0.077; 2/3 vs. 0, OR: 4.33, 95% CI: 1.38–13.61, P = 0.012). A nomogram was formed with both internal and external validation. Conclusions The post-NACT CTM score system served as a strong independent predictor for PFS and pCR in LAGC patients who received NACT. Further population-based studies are required to confirm our results.

Cu (II) is one of the most harmful heavy metals in the environment, due to the hazardous effects to aquatic organisms, plants, animals, and human beings. In this work, a novel modified magnetic biochar (MBC@APTES-PAMAM) was prepared by PAMAM dendrimer for the removal of Cu (II). The analysis of structural properties identically demonstrated that the abundant -NH2 groups were successfully loaded onto magnetic biochar surfaces. The sorption kinetics process of Cu (II) onto MBC@APTES-PAMAM was better fitted by the pseudo-second-order model. Langmuir isotherm model was better described for Cu (II) sorption process. MBC@APTES-PAMAM showed outstanding performances on Cu (II) removal, and the maximal adsorption amount was 251.81 mg/g. Adsorption experiment indicated that MBC@APTES-PAMAM had a higher Cu (II) removal efficiency under the pH range from 2 to 8. The adsorption capacity of Cu (II) onto MBC@APTES-PAMAM was decreased in the presence of competing ions. The existence of surfactant and humic acid had great influences on Cu (II) adsorption process. The Cu (II) adsorption mechanism was connected to the -NH2 by complexation reaction, due to the increased electrostatic attraction and bridging influences. In addition, MBC@APTES-PAMAM also exhibited comparable removal performances for COD (70.82%), TN (71.10%), TP (100%), NH3 N (55.03%) and Cu (II) (99.68%) in the practical treatment of landfill leachate. Overall, MBC@APTES-PAMAM is a green, low-cost, and high efficiency adsorbent for Cu (II) removal.

Biogas is the gaseous by product generated from anaerobic digestion (AD), which is mainly composed of methane and CO

ObjectiveLiver cancer is a significant contributor to global burden of cancer. Transcatheter arterial chemoembolization (TACE) is the standard of care for patients with unresectable liver cancer, and CalliSpheres, as novel drug-eluting bead (DEB) microspheres, have been found to be associated with a high tumor response rate. However, the outcomes after DEB-TACE treatment are not always satisfactory with tumor recurrence. Herein, we attempt to compare the clinical efficacy and safety of DEB-TACE with sorafenib and conventional TACE in treating advanced liver cancer.MethodsThe study retrospectively reviewed clinical records of 96 patients with liver cancer, among which there were 48 cases receiving DEB-TACE with sorafenib and 48 cases receiving conventional TACE. The physical properties of Callispheres were evaluated in HepG2 cells and a B6/J mouse model.ResultsDEB-TACE with Callispheres were demonstrated to effectively maintain stability and prolong the half-life of epirubicin. Compared with the patients receiving conventional TACE, those receiving DEB-TACE with sorafenib exhibited better patient outcomes with increased survival rate, reduced tumor volume, and declined levels of tumor markers. Additionally, DEB-TACE with Callispheres could effectively protect liver function, as well as reduce the toxic effects of loaded epirubicin, and its combination with sorafenib would not increase the incidence of adverse reactions.ConclusionDEB-TACE using CalliSpheres combined with sorafenib could prevent the progression of liver cancer and bring a better prognosis.

Background Deep venous thrombosis (DVT) is a relatively serious complication commonly seen in pregnant women, especially parturients with pregnancy-induced hypertension (PIH), whose incidence of DVT is higher. Because it can lead to pulmonary embolism (PE) and endanger the lives of patients, it has been paid much attention in clinic. Nursing plays an important role in the prevention and treatment of DVT. Early posture intervention can prevent postpartum DVT in hypertensive pregnant women, which has certain clinical value, but there is no evidence of evidence-based medicine. This study aims to systematically study the effectiveness and safety of postural intervention on prevention of deep venous thrombosis of lower extremity in postpartum women with PIH. Methods Use the computer to retrieve the English databases (PubMed, Embase, Web of Science, the Cochrane Library) and the Chinese databases (China Knowledge Network, Wanfang, Weipu, China Biomedical Database), in addition to the manual retrieval of Baidu academic, Google academic, from the construction of database to December 2020, for randomized controlled clinical studies of postural intervention on prevention of deep venous thrombosis of lower extremity in postpartum women with PIH. Two researchers independently extracted the data and evaluated the quality of the included research, and used RevMan5.3 software to do meta-analyze of the included literature. Results This study assessed the efficacy and safety of potential intervention on prevention of deep venous thrombosis of lower extremities with lower extremity hypertension by mean velocity of femoral venous flow in the lower extremities, lower extremity skin status, swelling level, and pain condition, lower extremity deep venous thrombosis rate, and incidence of pulmonary embolism. Conclusion This study will provide reliable evidence-based evidence for the clinical application of postural intervention on prevention of deep venous of lower extremity in Postpartum women with PIH. Osf registration number DOI 10.17605/OSF.IO/4NPKW.

As one of the fourth-generation fluoroquinolone antibiotics, moxifloxacin (MOX) has been frequently released to the aquatic environment, threatening local organisms. However, researches on its ecotoxicity to aquatic organisms are still limited. This study analyzed effects of MOX on the growth, photosynthesis and oxidative stress of two common types of freshwater microalgae, Chlorella sorokiniana and Scenedesmus dimorphus. The 96 h-EC

Background Laparoscopic gastrectomy (LG) for gastric cancer has rapidly developed and become more popular in recent decades. Additional high-quality randomized controlled trial (RCT) studies comparing LG versus open gastrectomy (OG) for gastric cancer (GC) have been published in recent years. An updated systematic review is warranted. The aim of our meta-analysis was to comprehensively evaluate the short- and long-term outcomes of LG versus OG for GC. Materials and methods The PubMed, Embase, Web of Science, and Cochrane Center Register of Controlled Trials databases were comprehensively searched to identify RCTs comparing LG versus OG for GC published between January 1994 and December 7, 2021. This study was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) and Cochrane Collaboration and the Quality of Reporting of Meta-analyses (QUORUM) guidelines. All RCTs comparing the short- and long-term outcomes of LG with those of OG were included. A random effects model was adopted with significant heterogeneity (I2 > 50%), while a fixed effects model was employed in all other cases (I2 ≤ 50%). Results A total of 26 RCTs with 8301 patients were included in this meta-analysis. The results indicated that the intraoperative complication rate was comparable between the LG group and the OG group (OR=1.14, 95% CI [0.76, 1.70], I2=0%, p=0.53). The LG group had fewer postoperative complications than the OG group (OR=0.65, 95% CI [0.57, 0.74], I2=26%, pI2=10%, p=0.10; OR=1.11, 95% CI [0.59, 2.09], I2=0%, p=0.74, respectively). The number of lymph nodes retrieved by the LG group was less than that of the OG group (MD=−1.51, 95% CI [−2.29, −0.74], I2=0%, pI2=23%, p=0.003), but the distal resection margin distance in the two groups was comparable (MD=−0.21, 95% CI [−0.47, 0.04], I2=0%, p=0.10). The time to first ambulation was shorter in the LG group than in the OG group (MD=−0.14, 95% CI [−.26, −0.01], I2=40%, p=0.03). The time to first flatus was also shorter in the LG group than in the OG group (MD=−0.15, 95% CI [−0.23, −0.07], I2=4%, p=0.0001). However, the first time on a liquid diet was comparable between the two groups (MD=−0.30, 95% CI [−0.64, 0.04], I2=88%, p=0.09). Furthermore, the postoperative length of stay was shorter in the LG group than in the OG group (MD=−1.26, 95% CI [−1.99, −0.53], I2=90%, p=0.0007). The 5-year overall survival (OS) was comparable between the two groups (HR=0.97, 95% CI [0.80, 1.17], I2=0%, p=0.73), and the 5-year disease-free survival (DFS) was also similar between the LG group and OG group (HR=1.08, 95% CI [0.77, 1.52], I2=0%, p=0.64). Conclusion LG is a technically safe and feasible alternative to OG with the advantages of a fewer postoperative complication rate, faster recovery of gastrointestinal function, and greater cosmetic benefit for patients with GC. Meanwhile, LG has comparable long-term outcomes to OG for GC.

This experiment aimed to investigate changes in enzyme activity, microbial succession, and nitrogen conversion caused by different initial carbon-to-nitrogen ratios of 25:1, 35:1 and 20:1 (namely CK, T1 and T2) during pig manure composting. The results showed that the lower carbon-to-nitrogen ratio (T2) after composting retained 19.64 g/kg of TN which was more than 16.74 and 17.32 g/kg in treatments of CK and T1, respectively, but excessive conversion of ammonium nitrogen to ammonia gas resulted in nitrogen loss. Additional straw in T1 could play the role as a bulking agent. After composting, TN in T1 retained the most, and TN contents were 63.51 %, 67.34 % and 56.24 % in CK, T1 and T2, respectively. Network analysis indicated that many types of microorganisms functioned as a whole community at various stages of nitrogen cycle. This study suggests that microbial community structure modification might be a good strategy to reduce ammonium nitrogen loss.

Soil pollution caused by petroleum pollutants from production trade activities in petroleum-related factories contributes serious threat to the environment and human health. Composting is technically-feasible and cost-effective in the biodegradation of heavy oil pollutants. This composting experiment was developed with four rice husk biochar (RHB) concentrations of 0 wt% (CK), 5 wt% (S1), 10 wt% (S2) and 15 wt% (S3) for the degradation of heavy oil. The results showed that RHB amendment could strengthen the degradation performance of heavy oil, and the degradation efficiencies for CK, S1, S2 and S3 were 59.67%, 65.00%, 73.29% and 74.82%, respectively. Microbial community succession process was investigated through high-throughput sequencing technology, and the RHB addition regulated bacterial community succession and further effectively facilitated the biodegradation of heavy oil in composting. This study substantiated that biochar materials-amended aerobic composting would be a promising strategy for the biodegradation of petroleum pollutants.

Inadequate sampling of lymph nodes could lead to stage migration and indicate a poor prognosis for gastric cancer after curative surgery. Some emerging novel predictors and the application of a nomogram could increase the accuracy of survival prediction. An international database regarding gastric cancer was employed as the primary cohort. The patients with inadequate (

To compare the short-term surgical outcomes of laparoscopic and open total/proximal gastrectomy using transorally inserted anvil (OrVilTM). Patients diagnosed with gastric cancer and underwent total or proximal gastrectomy using OrVilTM for reconstruction were included. Clinical and pathological characteristics, as well as postoperative outcomes, were analyzed. Propensity score matching was used to balance baseline factors. From April 2012 to April 2020, 199 patients at our center were included. A total of 166 underwent open total or proximal gastrectomy (OTG/OPG), and 33 underwent laparoscopic total or proximal gastrectomy (LTG/LPG). Twenty-seven patients from each group were paired with propensity score matching. The operation time was significantly shorter in the OTG/OPG group after matching. The overall complication rate and the incidence of each complication did not show significant differences between the two groups before and after matching. LTG/LPG and OTG/OPG using OrVilTM for the alimentary tract reconstruction are both feasible and can achieve similar short-term outcomes.

The crisis of water scarcity has become one of the most urgent issues to be settled. It is still a challenge to develop a highly efficient water purification technology. In this work, highly efficient capacitive removal of metal ions from wastewater was demonstrated by using Ti–C redox interfaces created by an atomic layer deposition (ALD) strategy. It has been illustrated that the TiO2 nanolayer was formed by reaction on the surface of porous carbon (PC) by using an ALD strategy, which could effectively build the Ti–C redox interfaces; and thus, the TiO2 nanolayer on PC was beneficial for facilitating ion diffusion/transmission and adsorption/insertion. Impressively, this process showed a deionization capacity of 38.54 mg g−1 in a 500 mg L−1 NaCl solution at 1.2 V. In addition, this process had excellent capacitive removal efficiency to treat complicated water containing multiple metal ions (K+, Mg2+, Ca2+, Ni2+, Co2+, Cu2+, Pb2+, Cd2+, Cr2+, Fe3+ and Al3+). This work has important practical implication for highly efficient removal of metal ions from wastewater.

Background: With the rapid aging of global population, the number of elderly patients with gastric cancer is increasing. This study aimed to evaluate short- and long-term outcomes after laparoscopi...

Impregnated graphite has attracted considerable attention and has been widely used as an ideal friction material in many fields. However, the influence of the friction temperature on its tribological properties has not been clearly studied; furthermore, the evolution mechanism of transferred tribofilm is unknown. In this study, the tribological properties of impregnated graphite were investigated at different friction temperatures, and the evolution of the carbon-based tribofilm was also determined. The results revealed that the tribological properties significantly improved with an increase in friction temperature. The friction coefficient and wear depth of impregnated graphite reduced by 68% and 75%, respectively, at a high temperature of 160 °C compared with those of non-impregnated graphite. The significant properties of the impregnated graphite can be attributed to a transferred carbon-based tribofilm with an ordered structure induced by the friction temperature, which uniformly and stably adsorbs on friction interfaces. This study provides an important basis for designing graphite-based friction materials with improved properties suited for industrial applications.

Simultaneous removal of heavy metals and organics from wastewater has always been an environmental problem with great concern. In this study, a novel ecofriendly bioborbent, magnetic chitosan beads immobilized Aspergillus sydowii (MCBAs) were synthesized and used to simultaneously remove trichlorfon (TCF) and Cd(II) from aqueous solution. MCBAs showed an increased special surface area (55.38 m2·g−1) through immobilizing A. sydowii and its saturation magnetization reached 14.62 emu·g−1. The equilibrium removal capacities of TCF and Cd(II) were 135.43 mg·g−1 and 56.40 mg·g−1 in the co-system with 200 mg·L−1 TCF and 50 mg·L−1 Cd(II), respectively. The removal capacities of TCF and Cd(II) were strongly depended on the immobilized A. sydowii spore concentration, initial concentrations of TCF and Cd(II), and MCBAs dose. TCF biodegradation intermediates were identified by gas chromatography–mass spectrometry system. Fourier transform infrared spectra displayed that –OH and –NH groups on MCBAs mainly participated in the Cd(II) sequestration and the C O stretching vibration was possibly related to the degradation intermediates of TCF. MCBAs exhibited excellent recyclability upto four cycles. Therefore, MCBAs are suitable and effective for the simultaneous removal of TCF and Cd(II) from wastewater.

Bacillus subtilis A-5 has the capabilities of high-molecular-weight γ-PGA production, antagonism to plant pathogenic fungi, and salt/alkaline tolerance. This multifunctional bacterium has great potential for enhancing soil fertility and plant security in agricultural ecosystem. The genome size of B. subtilis A-5 was 4,190,775 bp, containing 1 Chr and 2 plasmids (pA and pB) with 43.37% guanine-cytosine content and 4605 coding sequences. The γ-PGA synthase gene cluster was predicted to consist of pgsBCA and factor (pgsE). The γ-PGA-degrading enzymes were mainly pgdS, GGT, and cwlO. Nine gene clusters producing secondary metabolite substances, namely, four unknown function gene clusters and five antibiotic synthesis gene clusters (surfactin, fengycin, bacillibactin, subtilosin_A, and bacilysin), were predicted in the genome of B. subtilis A-5 using antiSMASH. In addition, B. subtilis A-5 contained genes related to carbohydrate and protein decomposition, proline synthesis, pyruvate kinase, and stress-resistant proteins. This affords significant insights into the survival and application of B. subtilis A-5 in adverse agricultural environmental conditions.

Purpose This study was conducted to compare the safety and short-term efficacy of gastrectomy after preoperative chemotherapy plus immunotherapy (PCIT) versus preoperative chemotherapy (PCT) in patients with advanced gastric cancer (AGC).Methods We retrospectively reviewed the data of patients with AGC who received PCIT or PCT at Peking University Cancer Hospital and Institute Gastrointestinal Cancer Center I between January 2019 and June 2021. The clinical characteristics were recorded, and short-term oncological outcomes were compared. Independent t-tests, Mann-Whitney U tests, Chi-square tests, and Fisher’s exact tests were used to calculate differences. The correlation analyses were performed using Pearson correlation. All p values were two-sided, and a p-value Results A total of 162 AGC patients were included in this study, including 25 patients receiving PCIT and 137 patients receiving PCT. There were no significant differences in preoperative treatment-related adverse events (TRAEs) between the PCIT group and the PCT group (p=0.088). Compared with the PCT group, the PCIT group had comparable postoperative functional recovery, with no significant differences in terms of the first aerofluxus time (p=0.349), the first defecating time (p=0.800), the first time on liquid diets (p=0.233), and length of stay (p=0.278). No significant differences were observed in terms of postoperative complications (p=0.952), postoperative pain intensity at 24, 48, and 72 hours (p=0.375, p=0.601, and p=0.821, respectively), and postoperative supplementary analgesic between the two groups (p=0.881). In addition, the postoperative complication rates were 33.3% following the laparoscopic approaches and 31.2% following the open approaches in the PCIT group, with no significant difference (p=1.000).Conclusions In patients with AGC, gastrectomy with D2 or D2+ lymphadenectomy after PCIT was comparable to PCT in terms of short-term oncological outcomes. The safety of LG was similar to that of OG in patients with AGC after PCIT.

Chemical fertilizer reduction combined with novel and green agricultural inputs has become an important practice to improve microecological health in agricultural production. Given the close linkages between rhizosphere processes and plant nutrition and productivity, understanding how fertilization impacts this critical zone is highly important for optimizing plant-soil interactions and crop fitness for agricultural sustainability. Here, by using a pot experimental system, we demonstrated that nitrogen fertilizer reduction and microbial agent application promoted plant fitness and altered the microbial community structure in the rhizosphere soil with the following treatments: no fertilization, CK; conventional chemical fertilizer, CF; 30% reduced nitrogen fertilizer, N; 30% reduced nitrogen fertilizer with pure γ-PGA, PGA; 30% reduced nitrogen fertilizer with

The gas–liquid miscible backflow pumping seal (G-LMBPHS) is a non-contact mechanical seal that is suitable for high-speed bearing chambers. However, the tribological properties and wear mechanisms of the frictional pair of G-LMBPHS in an oil–air environment have not yet been comprehensively studied. In this study, the tribological properties of six frictional pairs, consisting of three hard materials (18Cr2Ni4WA, Al2O3 coating, and Cr2O3 coating) and two soft materials (metal-impregnated graphite [Metal-IG] and resin-impregnated graphite [Resin-IG]), were analyzed using a disc-on-disc tribometer. An oil–air environment was created using a minimal quantity lubrication (MQL) system and a closed chamber. The results show that the COF of the four frictional pairs consisting of two coatings and two graphites decreases gradually with increasing rotational speed, and the frictional pairs composed of Al2O3 coating and Resin-IG and Cr2O3 coating and Resin-IG have the lowest COF between 0.022 and 0.03. Therefore, the frictional pairs of G-LMBPHS are in a mixed lubrication condition. The lubricant in the oil–air environment is adsorbed and stored in pits on the surface of graphite and coatings, enhancing the hydrodynamic effect of the spiral grooves and reducing the COF by up to 45%. Metal-IG has better wear resistance than Resin-IG, and the frictional pair consisting of Cr2O3 coating and Metal-IG has the lightest wear. This study provides an important basis for the selection of G-LMBPHS frictional pairs in oil–air environments. Validerad;2023;Nivå 2;2023-06-07 (hanlid);Funder: National Natural Science Foundation of China (52275170); State Key Laboratory of Tribology in Advanced Equipment (SKLTKF21A04)

Translational control exerts immediate effect on the composition, abundance, and integrity of the proteome. Ribosome-associated quality control (RQC) handles ribosomes stalled at the elongation and termination steps of translation, with ZNF598 in mammals and Hel2 in yeast serving as key sensors of translation stalling and coordinators of downstream resolution of collided ribosomes, termination of stalled translation, and removal of faulty translation products. The physiological regulation of RQC in general and ZNF598 in particular in multicellular settings is underexplored. Here we show that ZNF598 undergoes regulatory K63-linked ubiquitination and its level is upregulated upon mitochondrial stress in mammalian cells and Drosophila. Overexpression of ZNF598 protects against mitochondrial stress. In Drosophila models of neurodegenerative diseases and patient cells, ZNF598 overexpression aborted stalled translation of mitochondrial outer membrane-associated mRNAs and removed faulty translation products causal of disease. These results shed lights on the regulation of ZNF598 and its important role in mitochondrial homeostasis.