8 results on '"Thais Silva de Oliveira"'
Search Results
2. Synthetic (E)-3-Phenyl-5-(phenylamino)-2-styryl-1,3,4-thiadiazol-3-ium Chloride Derivatives as Promising Chemotherapy Agents on Cell Lines Infected with HTLV-1
- Author
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José Carlos Netto-Ferreira, Danilo Sousa-Pereira, Aurea Echevarria, Thais Silva de Oliveira, Otávio Augusto Chaves, Rojane de Oliveira Paiva, and Juliana Echevarria-Lima
- Subjects
Circular dichroism ,Magnetic Resonance Spectroscopy ,multiple spectroscopic ,mesoionic compounds ,Pharmaceutical Science ,01 natural sciences ,Jurkat cells ,Analytical Chemistry ,chemistry.chemical_compound ,Jurkat Cells ,Drug Discovery ,Leukemia-Lymphoma, Adult T-Cell ,Tissue Distribution ,Cytotoxicity ,Microwaves ,0303 health sciences ,Human T-lymphotropic virus 1 ,Circular Dichroism ,Mesoionic ,Human serum albumin ,HSA interaction ,Molecular Docking Simulation ,Chemistry (miscellaneous) ,Molecular Medicine ,medicine.symptom ,medicine.drug ,Protein Binding ,Biodistribution ,Stereochemistry ,Cell Survival ,Convergent synthesis ,Antineoplastic Agents ,Serum Albumin, Human ,Article ,lcsh:QD241-441 ,03 medical and health sciences ,Inhibitory Concentration 50 ,lcsh:Organic chemistry ,Chlorides ,Cell Line, Tumor ,medicine ,Humans ,Physical and Theoretical Chemistry ,030304 developmental biology ,Binding Sites ,010405 organic chemistry ,Organic Chemistry ,molecular docking ,0104 chemical sciences ,Mechanism of action ,chemistry ,HTLV-1 ,Drug Screening Assays, Antitumor ,Reactive Oxygen Species - Abstract
Synthesis of four compounds belonging to mesoionic class, (E)-3-phenyl-5-(phenylamino)-2-styryl-1,3,4-thiadiazol-3-ium chloride derivatives (5a&ndash, d) and their biological evaluation against MT2 and C92 cell lines infected with human T-cell lymphotropic virus type-1 (HTLV-1), which causes adult T-cell leukemia/lymphoma (ATLL), and non-infected cell lines (Jurkat) are reported. The compounds were obtained by convergent synthesis under microwave irradiation and the cytotoxicity was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Results showed IC50 values of all compounds in the range of 1.51&ndash, 7.70 M in HTLV-1-infected and non-infected cells. Furthermore, it was observed that 5b could induce necrosis after 24 h for Jurkat and MT2 cell lines. The experimental (fluorimetric method) and theoretical (molecular docking) results suggested that the mechanism of action for 5b could be related to its capacity to intercalate into DNA. Moreover, the preliminary pharmacokinetic profile of the studied compounds (5a&ndash, d) was obtained through human serum albumin (HSA) binding affinity using multiple spectroscopic techniques (circular dichroism, steady-state and time-resolved fluorescence), zeta potential and molecular docking calculations. The interaction HSA:5a&ndash, d is spontaneous and moderate (Ka ~ 104 M&minus, 1) via a ground-state association, without significantly perturbing both the secondary and surface structures of the albumin in the subdomain IIA (site I), indicating feasible biodistribution in the human bloodstream.
- Published
- 2020
3. [RETRACTED ARTICLE]: EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS
- Author
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Oscar Orlando Araya Fernandez, Rafaela de Souza Novo, Gabriele Escocia Marinho, Carlos Augusto Real Martinez, Carolina Mardegan Araya, José Aires Pereira, Thais Silva de Oliveira, Fábio Guilherme Campos, and Yara Tinoco Franceschi
- Subjects
Mucina-2 ,medicine.medical_specialty ,RD1-811 ,Colon ,medicine.medical_treatment ,Sucralfate ,RC799-869 ,Mucin 2 ,Gastroenterology ,Sucralfato ,Internal medicine ,Disclaimer ,medicine ,Fatty acids volatile ,Derivation ,Colitis ,Cólon ,Saline ,Feces ,Diversion colitis ,Mucin-2 ,business.industry ,General Medicine ,Diseases of the digestive system. Gastroenterology ,medicine.disease ,Immunohistochemistry ,Surgery ,Original Article ,Colite ,Ácidos graxos voláteis ,business ,medicine.drug - Abstract
Background: The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis. Aim: To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention. Methods: Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry). Results: The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p
- Published
- 2018
4. Protein Profile of Blood Monocytes is Altered in HTLV-1 Infected Patients: Implications for HAM/TSP Disease
- Author
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Thais Silva de Oliveira, Clarissa Rodrigues Nascimento, Marco A. Lima, Russolina B. Zingali, Juliana Echevarria-Lima, Dario E. Kalume, Denise de Abreu Pereira, Otávio de Melo Espíndola, Vanessa Sandim, and Ana Claudia Celestino Bezerra Leite
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0301 basic medicine ,Adult ,Male ,Proteomics ,Endothelium ,lcsh:Medicine ,Biology ,Virus ,Monocytes ,Histones ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,immune system diseases ,Cell Movement ,Heat shock protein ,hemic and lymphatic diseases ,Tropical spastic paraparesis ,medicine ,Cell Adhesion ,Humans ,Cell adhesion ,lcsh:Science ,Cytoskeleton ,Human T-lymphotropic virus 1 ,Multidisciplinary ,Monocyte ,lcsh:R ,virus diseases ,Viral Load ,medicine.disease ,HTLV-I Infections ,Paraparesis, Tropical Spastic ,Up-Regulation ,030104 developmental biology ,medicine.anatomical_structure ,Immunology ,lcsh:Q ,Female ,Asymptomatic carrier ,030217 neurology & neurosurgery - Abstract
Human T-cell lymphotropic virus type-1 (HTLV-1) is the etiological agent of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The endothelial breakdown and migration of leukocytes, including monocytes, to the spinal cord are involved in HAM/TSP development. Monocytes from HTLV-1-infected individuals exhibit important functional differences when compared to cells from uninfected donors. Using proteomic shot gun strategy, performed by nanoACQUITY-UPLC system, we analyzed monocytes isolated from peripheral blood of asymptomatic carriers (AC), HAM/TSP and uninfected individuals. 534 proteins were identified among which 376 were quantified by ExpressionE software. Our study revealed a panel of changes in protein expression linked to HTLV-1 infection. Upregulation of heat shock proteins and downregulation of canonical histone expression were observed in monocytes from HTLV-1-infected patients. Moreover, expression of cytoskeleton proteins was increased in monocytes from HTLV-1-infected patients, mainly in those from HAM/TSP, which was confirmed by flow cytometry and fluorescence microscopy. Importantly, functional assays demonstrated that monocytes from HAM/TSP patients present higher ability for adhesion and transmigration thought endothelium than those from AC and uninfected individuals. The major changes on monocyte protein profile were detected in HAM/TSP patients, suggesting that these alterations exert a relevant role in the establishment of HAM/TSP.
- Published
- 2017
5. EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS
- Author
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Oscar Orlando Araya Fernandez, Carlos Augusto Real Martinez, Rafaela de Souza Novo, Gabriele Escocia Marinho, Fábio Guilherme Campos, Carolina Mardegan Araya, Yara Tinoco Franceschi, Thais Silva de Oliveira, and José Aires Pereira
- Subjects
Mucina-2 ,Male ,medicine.medical_specialty ,RD1-811 ,Colon ,medicine.medical_treatment ,Sucralfate ,Enema ,RC799-869 ,Mucin 2 ,Gastroenterology ,Sucralfato ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Fatty acids volatile ,Derivation ,Colitis ,Intestinal Mucosa ,Rats, Wistar ,Cólon ,Saline ,Feces ,Diversion colitis ,Mucin-2 ,business.industry ,General Medicine ,Diseases of the digestive system. Gastroenterology ,medicine.disease ,Rats ,030220 oncology & carcinogenesis ,Surgery ,030211 gastroenterology & hepatology ,Colite ,Ácidos graxos voláteis ,Original Article ,business ,medicine.drug - Abstract
Background: The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis. Aim: To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention. Methods: Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry). Results: The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p
- Published
- 2016
6. CONTEÚDO TECIDUAL DAS FRAÇÕES GLICÍDICAS E PROTEICA DAS MUCINAS EM SEGMENTOS CÓLICOS SEM TRÂNSITO FECAL SUBMETIDOS À INTERVENÇÃO COM ÁCIDO 5‐AMINOSALICÍLICO
- Author
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Thais Silva de Oliveira, Rafaela de Souza Novo, Adrieli Heloisa Campardo Pansani, Yara Franceschi Saba, Gabriele Escocia Marinho, Carlos Augusto Real Martinez, and José Aires Pereira
- Subjects
Gastroenterology ,RC799-869 ,Diseases of the digestive system. Gastroenterology - Published
- 2017
7. EFEITOS DE CLISTERES CONTENDO MESALAZINA NO CONTEÚDO TECIDUAL DAS PROTEÍNAS CONSTITUINTES DAS JUNÇÕES DE ADESÃO INTERCELULAR NA COLITE DE EXCLUSÃO
- Author
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Carlos Augusto Real Martinez, Gabrielle Máira de Lima Matijascic, Thais Silva de Oliveira, Gabriele Escocia Marinho, Adrieli Heloisa Campardo Pansani, Rafaela de Souza Novo, and Yara Tinoco Franceschi
- Subjects
Gastroenterology ,RC799-869 ,Diseases of the digestive system. Gastroenterology - Published
- 2017
8. Growth of ornamental sunflowers in two growing seasons under semiarid conditions
- Author
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SILVA, S. D. P. da, CAVALCANTE, M. Z. B., SOUZA, G. P., OLIVEIRA, T. S., LIMA, R. da R., CHAVES, A. R. de M., SHEILA DANIELLA PEREIRA DA SILVA, UNIVASF, MÁRKILLA ZUNETE BECKMANN CAVALCANTE, UNIVASF, GRACIELLE PEIXOTO DE SOUZA, UNIVASF, THAIS SILVA DE OLIVEIRA, UNIVASF, RAYLLA DA ROCHA LIMA, UNIVASF, and AGNALDO RODRIGUES DE MELO CHAVES, CPATSA.
- Subjects
0106 biological sciences ,Growing season ,Context (language use) ,Biology ,01 natural sciences ,Applied Microbiology and Biotechnology ,Acclimatization ,Crop ,Sunflower hulls ,Ornamental plant ,Cultivar ,Girassol ,Planta Ornamental ,Semiárido ,04 agricultural and veterinary sciences ,Sunflower ,Horticulture ,Inflorescence ,040103 agronomy & agriculture ,Helianthus ,0401 agriculture, forestry, and fisheries ,Sunflower oil ,Animal Science and Zoology ,Agronomy and Crop Science ,Ornamental plants ,010606 plant biology & botany ,Food Science - Abstract
Sunflower is considered a temperate zone crop but it can perform well under varying climate conditions. In this context, the aim of this study was to evaluate the growth of ornamental sunflower cultivars for cutting in two growing seasons in semiarid conditions. Sunflowers cultivars were sown in a randomized complete design in a split plot system, with four blocks. Six cultivars were evaluated (?Bonito de outono sortido?, ?Sol noturno?, ?Sol vermelho?, ?Jardim amarelo alto?, ?Sunflower F1 sunbright supreme? and ?Sunflower F1 vincents choice?) in the main plots and two growing seasons in the subplots (GS1 ? hot weather and GS2 ? mild weather). Plant emergence percentage, plant height, stem diameter, number of leaves and internal inflorescence diameter were significant affected both by sunflowers cultivars and growing seasons. Emergence speed index and external inflorescence diameter were significantly affected by the interaction of cultivars and growing seasons. The obtained results indicated ?Sol vermelho? for cultivation in growing seasons with mild temperatures (GS2) and ?Sunflower F1 vincents choice? recommended for both growing seasons with mild climatic (GS2) and in warmer conditions (GS1). Although all other cultivars studied have not been indicated immediately, they may be considered for cultivation, but more studies are necessary for better acclimatization. Made available in DSpace on 2018-07-06T01:17:33Z (GMT). No. of bitstreams: 1 Agnaldo2018.pdf: 1225691 bytes, checksum: 1ab32155fb1dd24d3775f96278870c35 (MD5) Previous issue date: 2018-07-05
- Published
- 2018
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