289 results on '"Youming Lu"'
Search Results
2. Experimental and Computational Studies of Atomic Mobilities for fcc Al-Co-Cr Alloys
- Author
-
Genfeng Shang, Youming Lu, Jingjing Wang, and Xiao-Gang Lu
- Subjects
Materials Chemistry ,Metals and Alloys ,Condensed Matter Physics - Published
- 2022
3. Social isolation reinforces aging-related behavioral inflexibility by promoting neuronal necroptosis in basolateral amygdala
- Author
-
Juan Zhang, Dan Liu, Peng Fu, Zhi-Qiang Liu, Chuan Lai, Chun-Qing Yang, Kai Chen, Wen-Dai Bao, Fan Hu, Hui-Yun Du, Weili Yang, Jie Wang, Heng-Ye Man, Youming Lu, and Ling-Qiang Zhu
- Subjects
Neurons ,Mice ,Aging ,Cellular and Molecular Neuroscience ,Psychiatry and Mental health ,Social Isolation ,Basolateral Nuclear Complex ,Necroptosis ,Animals ,Molecular Biology - Abstract
Aging is characterized with a progressive decline in many cognitive functions, including behavioral flexibility, an important ability to respond appropriately to changing environmental contingencies. However, the underlying mechanisms of impaired behavioral flexibility in aging are not clear. In this study, we reported that necroptosis-induced reduction of neuronal activity in the basolateral amygdala (BLA) plays an important role in behavioral inflexibility in 5-month-old mice of the senescence-accelerated mice prone-8 (SAMP8) line, a well-established model with age-related phenotypes. Application of Nec-1s, a specific inhibitor of necroptosis, reversed the impairment of behavioral flexibility in SAMP8 mice. We further observed that the loss of glycogen synthase kinase 3α (GSK-3α) was strongly correlated with necroptosis in the BLA of aged mice and the amygdala of aged cynomolgus monkeys (Macaca fascicularis). Moreover, genetic deletion or knockdown of GSK-3α led to the activation of necroptosis and impaired behavioral flexibility in wild-type mice, while the restoration of GSK-3α expression in the BLA arrested necroptosis and behavioral inflexibility in aged mice. We further observed that GSK-3α loss resulted in the activation of mTORC1 signaling to promote RIPK3-dependent necroptosis. Importantly, we discovered that social isolation, a prevalent phenomenon in aged people, facilitated necroptosis and behavioral inflexibility in 4-month-old SAMP8 mice. Overall, our study not only revealed the molecular mechanisms of the dysfunction of behavioral flexibility in aged people but also identified a critical lifestyle risk factor and a possible intervention strategy.
- Published
- 2022
4. Correction: Synaptic dysfunction of Aldh1a1 neurons in the ventral tegmental area causes impulsive behaviors
- Author
-
Xinyan Li, Wenting Chen, Xian Huang, Wei Jing, Tongmei Zhang, Quntao Yu, Hongyan Yu, Hao Li, Qing Tian, Yumei Ding, and Youming Lu
- Subjects
Cellular and Molecular Neuroscience ,Neurology (clinical) ,Molecular Biology - Published
- 2023
5. Tau pathology epigenetically remodels the neuron-glial cross-talk in Alzheimer’s disease
- Author
-
Lan-Ting Zhou, Dan Liu, Hui-Cong Kang, Lu Lu, He-Zhou Huang, Wen-Qing Ai, Yang Zhou, Man-Fei Deng, Hao Li, Zhi-Qiang Liu, Wei-Feng Zhang, Ya-Zhuo Hu, Zhi-Tao Han, Hong-Hong Zhang, Jian-Jun Jia, Avijite Kumer Sarkar, Saldin Sharaydeh, Jie Wang, Heng-Ye Man, Marcel Schilling, Lars Bertram, Youming Lu, Ziyuan Guo, and Ling-Qiang Zhu
- Subjects
Multidisciplinary - Abstract
The neuron-glia cross-talk is critical to brain homeostasis and is particularly affected by neurodegenerative diseases. How neurons manipulate the neuron-astrocyte interaction under pathological conditions, such as hyperphosphorylated tau, a pathological hallmark in Alzheimer’s disease (AD), remains elusive. In this study, we identified excessively elevated neuronal expression of adenosine receptor 1 (Adora1 or A1R) in 3×Tg mice, MAPT P301L (rTg4510) mice, patients with AD, and patient-derived neurons. The up-regulation of A1R was found to be tau pathology dependent and posttranscriptionally regulated by Mef2c via miR-133a-3p. Rebuilding the miR-133a-3p/A1R signal effectively rescued synaptic and memory impairments in AD mice. Furthermore, neuronal A1R promoted the release of lipocalin 2 (Lcn2) and resulted in astrocyte activation. Last, silencing neuronal Lcn2 in AD mice ameliorated astrocyte activation and restored synaptic plasticity and learning/memory. Our findings reveal that the tau pathology remodels neuron-glial cross-talk and promotes neurodegenerative progression. Approaches targeting A1R and modulating this signaling pathway might be a potential therapeutic strategy for AD.
- Published
- 2023
6. A novel mouse model for polysynaptic retrograde tracing and rabies pathological research
- Author
-
Yige Song, Lanfang Li, Tian Ma, Bing Zhang, Jing Wang, Xiaomei Tang, Youming Lu, Aodi He, and Xinyan Li
- Abstract
Retrograde tracing is an important method for dissecting neuronal connections and mapping neural circuits. Over the past decades, several virus-based retrograde tracers have been developed and have contributed to displaying multiple neural circuits in the brain. However, most of the previously widely used viral tools have focused on mono-transsynaptic neural tracing within the central nervous system, with very limited options for achieving polysynaptic tracing between the central and peripheral nervous systems. In this study, we generated a novel mouse line, GT mice, in which both glycoprotein (G) and ASLV-A receptor (TVA) were expressed throughout the body. Using this mouse model, in combination with the well-developed rabies virus tools (RV-EnvA-ΔG) for monosynaptic retrograde tracing, polysynaptic retrograde tracing can be achieved. This allows functional forward mapping and long-term tracing. Furthermore, since the G-deleted rabies virus can travel upstream against the nervous system as the original strain, this mouse model can also be used for rabies pathological studies.
- Published
- 2023
7. Observation of table-like magnetocaloric effect and large refrigerant capacity in Nd6Fe13Pd1–Cu compounds
- Author
-
Yusong Du, Chaohua Zhang, Junqin Li, Jiang Wang, Guanghui Rao, Youming Lu, and Gang Cheng
- Subjects
Materials science ,Ericsson cycle ,Thermodynamics ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Magnetic hysteresis ,01 natural sciences ,0104 chemical sciences ,law.invention ,Refrigerant ,Tetragonal crystal system ,Ferromagnetism ,Geochemistry and Petrology ,law ,Phase (matter) ,Magnetic refrigeration ,Antiferromagnetism ,0210 nano-technology - Abstract
The table-like magnetocaloric effect is significant for the magnetic refrigeration applications above 20 K based on the Ericsson cycle. Herein, we prepared a series of Nd6Fe13Pd1–xCux (x=0.05, 0.1, 0.15) compounds by the arc-melting method. These compounds show the single crystalline phase in the tetragonal Nd6Fe13Si-type structure with the space group I4/mcm. A magnetic phase transition from ferromagnetism to antiferromagnetism and a metamagnetic transition from the antiferromagnetic state to the ferromagnetic state were observed in each of the compounds. The compounds exhibit table-like magnetocaloric effects with large refrigerant capacities. A constant ΔSM in a temperature span of 40 K in the Nd6Fe13Pd0.85Cu0.15 compound was observed. For a field change of 0–5 T, the peak values of –ΔSM for the Nd6Fe13Pd0.95Cu0.05, Nd6Fe13Pd0.90Cu0.10, and Nd6Fe13Pd0.85Cu0.15 compounds were estimated to be 4.8, 4.6 and 4.4 J/(kg·K) with corresponding refrigerant capacity values of 323, 331 and 316 J/kg, respectively. The obtained table-like magnetocaloric effects with large refrigerant capacities as well as fairly small thermal and magnetic hysteresis deem these series of compounds good candidates for single-phase magnetic refrigeration based on the Ericsson cycle.
- Published
- 2022
8. Enhanced Performance of GaN Schottky Barrier Diodes by Oxygen Plasma Treatment
- Author
-
Xiaobo Li, Feng Lin, Junye Wu, Zhiyue Zhang, Lijun Song, Taofei Pu, Xicong Li, Xinnan Lin, Youming Lu, and Xinke Liu
- Subjects
Electrical and Electronic Engineering ,Electronic, Optical and Magnetic Materials - Published
- 2022
9. EPAC2 knockout causes abnormal tau pathology through calpain-mediated CDK5 activation
- Author
-
De-Yi Liu, He-Zhou Huang, Ke Li, Youming Lu, and Ling-Qiang Zhu
- Subjects
mental disorders - Abstract
Tau pathology, including aberrant tau hyperphosphorylation, aggregation, and mislocalization, is implicated in many neurodegenerative disorders, including Alzheimer’s disease (AD), which is the most prevalent dementia among the elderly. A better understanding of the molecular mechanisms underlying tau pathology should help advance therapies for neurodegenerative diseases. Perturbations in cyclic adenosine monophosphate (cAMP)-dependent signaling play an important role in the pathophysiology of numerous neurological diseases. EPAC2 is an intracellular cAMP receptor whose expression is downregulated in AD. However, the involvement and role of EPAC2 in tau pathology remain unclear. In this study, we report for the 1st time that EPAC2 is downregulated in the hippocampus of Tg2576 mice, a widely used transgenic mouse model of familial AD. Furthermore, genetic deletion of EPAC2 resulted in abnormal hyperphosphorylation at multiple sites on tau. Aberrant tau aggregation and abnormal neuronal morphology were also detected in these EPAC2−/− mice. Administration of inhibitors of CDK5 or calpain effectively rescued the tau pathology in EPAC2−/− mice. This suggests that the activation of CDK5 by calpain plays an important role in the development of tau pathology in these EPAC2−/− mice. Collectively, our findings demonstrate a direct link between EPAC2 and tau pathology, and suggest that the EPAC2 and calpain/CDK5 signaling pathways may have potential as therapeutic targets for AD.
- Published
- 2022
10. LINC00662 Promotes Proliferation and Invasion and Inhibits Apoptosis of Glioma Cells Through miR-483-3p/SOX3 Axis
- Author
-
Lei Yuan, Pengqi Zhang, Youming Lu, Aijun Zhang, and Xin Chen
- Subjects
Carcinogenesis ,SOXB1 Transcription Factors ,Apoptosis ,Bioengineering ,Glioma ,General Medicine ,Applied Microbiology and Biotechnology ,Biochemistry ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Cell Movement ,Cell Line, Tumor ,Humans ,RNA, Long Noncoding ,Molecular Biology ,Cell Proliferation ,Biotechnology - Abstract
LINC00662 plays a prominent role in the carcinogenesis and progression of diverse cancers. However, its biological functions in glioma are still unclear. LINC00662 expression in glioma tissue samples and cell lines was examined by quantitative real-time polymerase chain reaction. The correlation between LINC00662 expression and the clinical characteristics of 50 patients with glioma was analyzed. LINC00662 knockdown and overexpression cell lines were constructed, and the effects of LINC00662 on the proliferation, invasion, and apoptosis of glioma cells were evaluated by cell counting kit-8, 5-ethynyl-2'-deoxyuridine, Transwell, and flow cytometry assays, respectively. Besides, the relationships among LINC00662, miR-483-3p, and sex-determining region Y-box 3 (SOX3) were assessed by dual-luciferase reporter assay and RNA immunoprecipitation assay. Western blot was used to detect the regulatory effects of LINC00662 and miR-483-3p on SOX3 expression in glioma cells. LINC00662 expression level was elevated in glioma tissues and cell lines compared to that in normal tissues and cell lines. LINC00662 high expression was associated with the adverse prognosis of patients with glioma. Knockdown of LINC00662 repressed the proliferation and invasion of glioma cells, and promoted apoptosis. Additionally, it was revealed that LINC00662 acted as the molecular sponge of miR-483-3p, and SOX3 was verified as a direct target of miR-483-3p. The inhibition of miR-483-3p expression and SOX3 overexpression reversed the biological effects of LINC00662 knockdown on glioma cells. This study reports the key regulatory role of LINC00662/miR-483-3p/SOX3 axis in the tumorigenesis and progression of glioma, bringing novel insights into the underlying mechanisms of glioma.
- Published
- 2022
11. Chk1 Inhibition Ameliorates Alzheimer’s Disease Pathogenesis and Cognitive Dysfunction Through CIP2A/PP2A Signaling
- Author
-
Wenting Hu, Zhuoqun Wang, Huiliang Zhang, Yacoubou Abdoul Razak Mahaman, Fang Huang, Dongli Meng, Ying Zhou, Shiyi Wang, Nan Jiang, Jing Xiong, Jukka Westermarck, Youming Lu, Jianzhi Wang, Xiaochuan Wang, Yangping Shentu, and Rong Liu
- Subjects
Pharmacology ,Amyloid beta-Peptides ,Intracellular Signaling Peptides and Proteins ,Membrane Proteins ,Mice, Transgenic ,Neurodegenerative Diseases ,tau Proteins ,Autoantigens ,environment and public health ,Disease Models, Animal ,Mice ,enzymes and coenzymes (carbohydrates) ,Alzheimer Disease ,Checkpoint Kinase 1 ,Animals ,Humans ,Cognitive Dysfunction ,Pharmacology (medical) ,Protein Phosphatase 2 ,Neurology (clinical) ,Phosphorylation ,biological phenomena, cell phenomena, and immunity ,Signal Transduction - Abstract
Alzheimer’s disease (AD) is the most common neurodegenerative disease with limited therapeutic strategies. Cell cycle checkpoint protein kinase 1 (Chk1) is a Ser/Thr protein kinase which is activated in response to DNA damage, the latter which is an early event in AD. However, whether DNA damage-induced Chk1 activation participates in the development of AD and Chk1 inhibition ameliorates AD-like pathogenesis remain unclarified. Here, we demonstrate that Chk1 activity and the levels of protein phosphatase 2A (PP2A) inhibitory protein CIP2A are elevated in AD human brains, APP/PS1 transgenic mice, and primary neurons with Aβ treatment. Chk1 overexpression induces CIP2A upregulation, PP2A inhibition, tau and APP hyperphosphorylation, synaptic impairments, and cognitive memory deficit in mice. Moreover, Chk1 inhibitor (GDC0575) effectively increases PP2A activity, decreases tau phosphorylation, and inhibits Aβ overproduction in AD cell models. GDC0575 also reverses AD-like cognitive deficits and prevents neuron loss and synaptic impairments in APP/PS1 mice. In conclusion, our study uncovers a mechanism by which DNA damage-induced Chk1 activation promotes CIP2A-mediated tau and APP hyperphosphorylation and cognitive dysfunction in Alzheimer’s disease and highlights the therapeutic potential of Chk1 inhibitors in AD.
- Published
- 2022
12. Fabrication of high-performance ZnO-based thin-film transistors by Mg/H co-doping at room temperature
- Author
-
Zongjin Jiang, Dongbo Yin, Deliang Zhu, Wangying Xu, Shun Han, Ming Fang, Wenjun Liu, Peijiang Cao, Xinke Liu, and Youming Lu
- Subjects
Electrical and Electronic Engineering ,Condensed Matter Physics ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials - Published
- 2022
13. Sodium–calcium exchanger isoform-3 targeted Withania somnifera (L.) Dunal therapeutic intervention ameliorates cognition in the 5xFAD mouse model of Alzheimer’s disease
- Author
-
Henok Kessete Afewerky, Hao Li, Tongmei Zhang, Xinyan Li, Yacoubou Abdoul Razak Mahaman, Limin Duan, Pengwei Qin, Jiequn Zheng, Lei Pei, and Youming Lu
- Subjects
Multidisciplinary ,Medical research ,Science ,Medicine ,Diseases ,Sodium-Calcium Exchanger ,Article ,Neuroscience - Abstract
The third isoform of the Na+–Ca2+ exchanger (NCX3) is crucial for a physiological fine-tuning of the Ca2+ fluxes in excitable tissues. In this view, the NCX3 accounts for the aberrant Ca2+ influx seen during neuronal excitotoxicity, such as in Alzheimer’s disease (AD). However, little is known about NCX3 regulation and functional properties. Withania somnifera (L.) Dunal (W. somnifera), a traditional indigenous plant widely recognized for having numerous medicinal values, was undertaken to determine its potential therapeutic benefit against aggregated Aβ1–42-induced NCX3 dysregulation and the thereof cognition impairment in 5xFAD mice. The undertaken sourced dried roots of authenticated W. somnifera physicochemical compositional tests satisfied standards of pharmacognostic quality, and further phytochemical analysis of the roots methanol extract revealed the roots constitute several antioxidants. Following an intra-gastric gavage administration of synthesized W. somnifera roots methanolic extract from postnatal day 30 (P30) to P75, in vivo cognitional studies and then neurochemical examinations of the NCX3 expression level, Aβ plaque deposition, and antioxidant activities in the AD-associated brain regions of 4-month-old 5xFAD mice suggests that the oxidative stress normalizing effects of W. somnifera constituents, operating on the NCX3, may have a therapeutic role in the improvement of cognition in AD.
- Published
- 2022
14. Au@ZnO/rGO nanocomposite-based ultra-low detection limit highly sensitive and selective NO2 gas sensor
- Author
-
PeiJiang Cao, YongZhi Cai, Dnyandeo Pawar, Shun Han, WangYing Xu, Ming Fang, XinKe Liu, YuXiang Zeng, WenJun Liu, YouMing Lu, and DeLiang Zhu
- Subjects
Materials Chemistry ,General Chemistry - Abstract
An Au@ZnO/rGO nanocomposite demonstrates a sensitive, selective, and ultra-low detection limit for NO2 sensing (5 ppb-1 ppm) at 60 °C.
- Published
- 2022
15. A novel H129-based anterograde monosynaptic tracer exhibits features of strong labeling intensity, high tracing efficiency, and reduced retrograde labeling
- Author
-
Hong Yang, Feng Xiong, Hai-Bin Qin, Qun-Tao Yu, Jin-Yan Sun, Hai-Wen Zhao, Dong Li, Youtong Zhou, Fu-Kun Zhang, Xiao-Wen Zhu, Tong Wu, Man Jiang, Xiangmin Xu, Youming Lu, Hong-Jie Shen, Wen-Bo Zeng, Fei Zhao, and Min-Hua Luo
- Subjects
Clinical Sciences ,Herpesvirus 1, Human ,Tracing efficiency ,Labeling intensity ,Cellular and Molecular Neuroscience ,Mice ,Genetics ,Animals ,Mutant gK pseudotyping ,RC346-429 ,Molecular Biology ,Retrograde labeling ,Neurons ,Neurology & Neurosurgery ,Herpesvirus 1 ,H129-dgK-G4 ,Neurosciences ,RC952-954.6 ,Brain ,Alzheimer's disease ,Anterograde monosynaptic tracer ,Glycoprotein K ,Axons ,Brain Disorders ,Axon terminal invasion ,Connectivity quantitation ,Geriatrics ,Anterograde specificity ,Glycoprotein K (gK) ,Sexually Transmitted Infections ,Neurology (clinical) ,Neurology. Diseases of the nervous system ,Alzheimer’s disease ,Human ,Research Article - Abstract
Background Viral tracers are important tools for mapping brain connectomes. The feature of predominant anterograde transneuronal transmission offers herpes simplex virus-1 (HSV-1) strain H129 (HSV1-H129) as a promising candidate to be developed as anterograde viral tracers. In our earlier studies, we developed H129-derived anterograde polysynaptic tracers and TK deficient (H129-dTK) monosynaptic tracers. However, their broad application is limited by some intrinsic drawbacks of the H129-dTK tracers, such as low labeling intensity due to TK deficiency and potential retrograde labeling caused by axon terminal invasion. The glycoprotein K (gK) of HSV-1 plays important roles in virus entry, egress, and virus-induced cell fusion. Its deficiency severely disables virus egress and spread, while only slightly limits viral genome replication and expression of viral proteins. Therefore, we created a novel H129-derived anterograde monosynaptic tracer (H129-dgK) by targeting gK, which overcomes the limitations of H129-dTK. Methods Using our established platform and pipeline for developing viral tracers, we generated a novel tracer by deleting the gK gene from the H129-G4. The gK-deleted virus (H129-dgK-G4) was reconstituted and propagated in the Vero cell expressing wildtype H129 gK (gKwt) or the mutant gK (gKmut, A40V, C82S, M223I, L224V, V309M), respectively. Then the obtained viral tracers of gKmut pseudotyped and gKwt coated H129-dgK-G4 were tested in vitro and in vivo to characterize their tracing properties. Results H129-dgK-G4 expresses high levels of fluorescent proteins, eliminating the requirement of immunostaining for imaging detection. Compared to the TK deficient monosynaptic tracer H129-dTK-G4, H129-dgK-G4 labeled neurons with 1.76-fold stronger fluorescence intensity, and visualized 2.00-fold more postsynaptic neurons in the downstream brain regions. gKmut pseudotyping leads to a 77% decrease in retrograde labeling by reducing axon terminal invasion, and thus dramatically improves the anterograde-specific tracing of H129-dgK-G4. In addition, assisted by the AAV helper trans-complementarily expressing gKwt, H129-dgK-G4 allows for mapping monosynaptic connections and quantifying the circuit connectivity difference in the Alzheimer’s disease and control mouse brains. Conclusions gKmut pseudotyped H129-dgK-G4, a novel anterograde monosynaptic tracer, overcomes the limitations of H129-dTK tracers, and demonstrates desirable features of strong labeling intensity, high tracing efficiency, and improved anterograde specificity.
- Published
- 2022
16. Recessed Anode AlGaN/GaN Schottky Barrier Diode for Temperature Sensor Application
- Author
-
Jin-Ping Ao, Junye Wu, Xinke Liu, Xiaobo Li, Youming Lu, Taofei Pu, and Jiaying Yang
- Subjects
Materials science ,business.industry ,Schottky barrier ,Wide-bandgap semiconductor ,Linearity ,Schottky diode ,Thermionic emission ,Electronic, Optical and Magnetic Materials ,Anode ,Rectification ,Optoelectronics ,Electrical and Electronic Engineering ,business ,Voltage - Abstract
AlGaN/GaN Schottky barrier diode (SBD) temperature sensor with low turn-on voltage was fabricated by employing the recessed anode structure. This AlGaN/GaN SBD demonstrates good rectification in a broad temperature scope from 298 to 473 K. Compared with common planar diode, the recessed anode SBD shows a relatively lower turn-on voltage and better Schottky contact characteristics. The temperature-dependent forward voltage at a fixed current displays great linearity, contributing to a sensitivity of about 1.0 mV/K. The calculated sensitivities exhibit the downtrend by increasing current level, which is in agreement with the thermionic emission (TE) model. The recessed anode AlGaN/GaN SBDs show good potential in temperature sensor application.
- Published
- 2021
17. An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing
- Author
-
Feng Xiong, Hong Yang, Yi-Ge Song, Hai-Bin Qin, Qing-Yang Zhang, Xian Huang, Wei Jing, Manfei Deng, Yang Liu, Zhixiang Liu, Yin Shen, Yunyun Han, Youming Lu, Xiangmin Xu, Todd C. Holmes, Minmin Luo, Fei Zhao, Min-Hua Luo, and Wen-Bo Zeng
- Subjects
Neurons ,Multidisciplinary ,Herpesvirus 1 ,Connectome ,Genetics ,Neurosciences ,General Physics and Astronomy ,General Chemistry ,Herpesvirus 1, Human ,Nerve Net ,General Biochemistry, Genetics and Molecular Biology ,Human - Abstract
Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monosynaptic H129Amp tracer system based on HSV-1 strain H129. The H129Amp tracer system consists of two components: an H129-dTK-T2-pacFlox helper which assists H129Amp tracer’s propagation and transneuronal monosynaptic transmission. The shared viral features of tracer/helper allow for simultaneous single-injection and subsequent high expression efficiency from multiple-copy of expression cassettes in H129Amp tracer. These improvements of H129Amp tracer system shorten experiment duration from 28-day to 5-day for fast-bright monosynaptic tracing. The lack of toxic viral genes in the H129Amp tracer minimizes toxicity in postsynaptic neurons, thus offering the potential for functional anterograde mapping and long-term tracer delivery of genetic payloads. The H129Amp tracer system is a powerful tracing tool for revealing neuronal connectomes.
- Published
- 2022
18. Aβ 1‐42 ‐related sodium‐calcium exchanger isoform‐3 downregulation in models of Alzheimer’s disease: Therapeutic significance of Withania somnifera roots extract
- Author
-
Henok Kessete Afewerky, Hao Li, Tongmei Zhang, Xinyan Li, Yacoubou Abdoul Razak Mahaman, Limin Duan, Pengwei Qin, Jiequn Zheng, Lei Pei, and Youming Lu
- Subjects
Psychiatry and Mental health ,Cellular and Molecular Neuroscience ,Developmental Neuroscience ,Epidemiology ,Health Policy ,Neurology (clinical) ,Geriatrics and Gerontology - Published
- 2022
19. Ultra-high sensitive and ultra-low NO2 detection at low-temperature based on ultrathin In2O3 nanosheets
- Author
-
Zeng Yuxiang, Deliang Zhu, Xinke Liu, Dnyandeo Pawar, Wenjun Liu, Peijiang Cao, Ch. N. Rao, YongZhi Cai, Ming Fang, Shun Han, RongGuan Chen, Youming Lu, and WangYin Xu
- Subjects
Detection limit ,Materials science ,Oxide ,chemistry.chemical_element ,Repeatability ,Condensed Matter Physics ,Oxygen ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Phase (matter) ,Nitrogen dioxide ,Electrical and Electronic Engineering ,Selectivity ,Indium - Abstract
In this study, the high oxygen vacancy-rich ultrathin indium oxide (In2O3) nanosheets are synthesized by low-cost and environmental-friendly solvothermal method. The gas sensing properties of In2O3 nanosheets to low concentration of nitrogen dioxide (NO2) (concentration in 5 ppb to 5 ppm) at 50 °C are investigated. All In2O3 samples are successfully characterized using various techniques to obtain the information related to phase, morphological, oxygen vacancies, and surface area, etc. It is observed that among all the samples, the In2O3-350 exhibited benchmarked response of around 3223 at 2 ppm NO2 gas, with theoretical detection limit down to ppb level. The sensor has shown superior sensing characteristics, such as wide sensing bandwidth, excellent selectivity, high long-term stability, and good repeatability. It is considered that the high oxygen deficiency present on In2O3 nanosheets acts as favorable reaction sites for such remarkable sensing performance, and therefore, these improved gas-sensing properties make the In2O3 nanosheets suitable to detect NO2 at low-temperature.
- Published
- 2021
20. Gut dysbiosis impairs hippocampal plasticity and behaviors by remodeling serum metabolome
- Author
-
Guoqiang, Liu, Quntao, Yu, Bo, Tan, Xiao, Ke, Chen, Zhang, Hao, Li, Tongmei, Zhang, and Youming, Lu
- Subjects
Adult ,Microbiology (medical) ,Mice ,Infectious Diseases ,Autism Spectrum Disorder ,Metabolome ,Gastroenterology ,Animals ,Dysbiosis ,Humans ,Hippocampus ,Microbiology ,Gastrointestinal Microbiome - Abstract
Accumulating evidence suggests that gut microbiota as a critical mediator of gut-brain axis plays an important role in human health. Altered gut microbial profiles have been implicated in increasing the vulnerability of psychiatric disorders, such as autism, depression, and schizophrenia. However, the cellular and molecular mechanisms underlying the association remain unknown. Here, we modified the gut microbiome with antibiotics in newborn mice, and found that gut microbial alteration induced behavioral impairment by decreasing adult neurogenesis and long-term potentiation of synaptic transmission, and altering the gene expression profile in hippocampus. Reconstitution with normal gut flora produced therapeutic effects against both adult neurogenesis and behavioral deficits in the dysbiosis mice. Furthermore, our results show that circulating metabolites changes mediate the effect of gut dysbiosis on hippocampal plasticity and behavior outcomes. Elevating the serum 4-methylphenol, a small aromatic metabolite produced by gut bacteria, was found to induce autism spectrum disorder (ASD)-like behavior impairment and hippocampal dysfunction. Together our finding demonstrates that early-life gut dysbiosis and its correlated metabolites change contribute to hippocampal dysfunction and behavior impairment, hence highlight the potential microbiome-mediated therapies for treating psychiatric disorders.
- Published
- 2022
21. Influence of exposed facets, morphology and hetero-interfaces of BiVO4 on photocatalytic water oxidation: A review
- Author
-
Rayees Ahmad Rather, Ming Fang, Youming Lu, Akansha Mehta, Matjaz Valant, and Wenjun Liu
- Subjects
Materials science ,Hydrogen ,Renewable Energy, Sustainability and the Environment ,business.industry ,Band gap ,Energy Engineering and Power Technology ,chemistry.chemical_element ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,Fuel Technology ,Semiconductor ,chemistry ,Bismuth vanadate ,Photocatalysis ,Water splitting ,Facet ,0210 nano-technology ,business ,Photocatalytic water splitting - Abstract
The photocatalytic water splitting has a stupendous role to generate renewable hydrogen. However, the overall water splitting reaction is limited by the sluggish oxidation step. Bismuth vanadate (BiVO4) has been identified as one of the most promising n-type semiconductors for photocatalytic water oxidation due to its small energy gap and favorable band alignment. Thus, it is necessary to summarize the recent progress done on BiVO4 for the guidance of future research. In this review, we have discussed recent strategies that have been adopted to boost its photoconversion efficiency at three different levels: (1) facet control, (2) morphological control and, (3) interfacial control. The roles of high indexed facets, anisotropic morphologies, and mediator-based Z schemes are comprehensively discussed and the emphasis is given to find the suitable structural, morphological, and interfacial combination of BiVO4 for efficient water oxidation.
- Published
- 2021
22. Identification of an endogenous glutamatergic transmitter system controlling excitability and conductivity of atrial cardiomyocytes
- Author
-
Li Li, Hongyu Liu, Yi-Han Chen, Qicheng Zou, Jian Yang, Youming Lu, Xingdong Gu, Ke Xiong, Qiang Ji, Luying Peng, Dandan Liang, Jinfeng Xue, Lingling Wang, Xiaoyu He, Duanyang Xie, Luxin Wang, Xuling Su, Yi Liu, Guanghua Wang, and Xueting Gao
- Subjects
Induced Pluripotent Stem Cells ,Action Potentials ,Endogeny ,030204 cardiovascular system & hematology ,Article ,03 medical and health sciences ,Glutamatergic ,0302 clinical medicine ,Atrial Fibrillation ,Animals ,Humans ,Myocytes, Cardiac ,Heart Atria ,GRIA3 ,Induced pluripotent stem cell ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,biology ,Glutamate receptor ,Cell Biology ,Rats ,Cell biology ,Electrophysiology ,cardiovascular system ,biology.protein ,Excitatory postsynaptic potential ,Ionotropic effect - Abstract
As an excitatory transmitter system, the glutamatergic transmitter system controls excitability and conductivity of neurons. Since both cardiomyocytes and neurons are excitable cells, we hypothesized that cardiomyocytes may also be regulated by a similar system. Here, we have demonstrated that atrial cardiomyocytes have an intrinsic glutamatergic transmitter system, which regulates the generation and propagation of action potentials. First, there are abundant vesicles containing glutamate beneath the plasma membrane of rat atrial cardiomyocytes. Second, rat atrial cardiomyocytes express key elements of the glutamatergic transmitter system, such as the glutamate metabolic enzyme, ionotropic glutamate receptors (iGluRs), and glutamate transporters. Third, iGluR agonists evoke iGluR-gated currents and decrease the threshold of electrical excitability in rat atrial cardiomyocytes. Fourth, iGluR antagonists strikingly attenuate the conduction velocity of electrical impulses in rat atrial myocardium both in vitro and in vivo. Knockdown of GRIA3 or GRIN1, two highly expressed iGluR subtypes in atria, drastically decreased the excitatory firing rate and slowed down the electrical conduction velocity in cultured human induced pluripotent stem cell (iPSC)-derived atrial cardiomyocyte monolayers. Finally, iGluR antagonists effectively prevent and terminate atrial fibrillation in a rat isolated heart model. In addition, the key elements of the glutamatergic transmitter system are also present and show electrophysiological functions in human atrial cardiomyocytes. In conclusion, our data reveal an intrinsic glutamatergic transmitter system directly modulating excitability and conductivity of atrial cardiomyocytes through controlling iGluR-gated currents. Manipulation of this system may open potential new avenues for therapeutic intervention of cardiac arrhythmias.
- Published
- 2021
23. Endoscopic Surgery Versus Drainage of Hematoma under Robot CAS-R-2 for Treatment of Basal Ganglia Hemorrhage
- Author
-
Dongdong Zou, Xin Chen, Pengqi Zhang, Youming Lu, Aijun Zhang, Lei Yuan, and Fance Wei
- Abstract
Aims: To compare Endoscopic Surgery and Robot CAS-R-2 drainage for surgical treatment of basal ganglia hypertensive intracerebral hemorrhage. Methods: Fifty consecutive basal ganglia hemorrhage patients were reviewed retrospectively. The primary and secondary outcomes were 6-months mortality and 6-months modified Rankin Scale score, which were assessed by a multivariate logistic regression mode.Results: Compared with the Robot CAS-R-2 group, the 6-month prognosis was significantly higher in the endoscopic evacuation group (OR 5.912, 95% CI 1.014-34.459). Preoperative hematoma volume (OR = 0.931, 95% CI 0.875-0.990) and IVH (OR = 0.578, 95% CI0.396-0.844) were independent predictors for 6-month prognosis. No significant difference in the mortality was found in the two groups (OR 0.501, 95% CI 0.192-1.308).Conclusion: Endoscopic hematoma removal significantly improved the 6-month prognosis compared with the drainage of hematoma under Robot CAS-R-2.
- Published
- 2022
24. PINK1-mediated Drp1S616 phosphorylation modulates synaptic development and plasticity via promoting mitochondrial fission
- Author
-
Qingtao Gao, Runyi Tian, Hailong Han, Jesse Slone, Caifang Wang, Xiao Ke, Tongmei Zhang, Xiangyu Li, Yuhong He, Panlin Liao, Fang Wang, Ye Chen, Shiqing Fu, Kexuan Zhang, Fangfang Zeng, Yingxuan Yang, Zhuo Li, Jieqiong Tan, Jiada Li, Youming Lu, Taosheng Huang, Zhonghua Hu, and Zhuohua Zhang
- Subjects
Cancer Research ,Genetics - Abstract
Dynamic change of mitochondrial morphology and distribution along neuronal branches are essential for neural circuitry formation and synaptic efficacy. However, the underlying mechanism remains elusive. We show here that Pink1 knockout (KO) mice display defective dendritic spine maturation, reduced axonal synaptic vesicles, abnormal synaptic connection, and attenuated long-term synaptic potentiation (LTP). Drp1 activation via S616 phosphorylation rescues deficits of spine maturation in Pink1 KO neurons. Notably, mice harboring a knockin (KI) phosphor-null Drp1S616A recapitulate spine immaturity and synaptic abnormality identified in Pink1 KO mice. Chemical LTP (cLTP) induces Drp1S616 phosphorylation in a PINK1-dependent manner. Moreover, phosphor-mimetic Drp1S616D restores reduced dendritic spine localization of mitochondria in Pink1 KO neurons. Together, this study provides the first in vivo evidence of functional regulation of Drp1 by phosphorylation and suggests that PINK1-Drp1S616 phosphorylation coupling is essential for convergence between mitochondrial dynamics and neural circuitry formation and refinement.
- Published
- 2022
25. Great Enhancement Effect of 20–40 nm Ag NPs on Solar-Blind UV Response of the Mixed-Phase MgZnO Detector
- Author
-
Shun Han, Deliang Zhu, Wangying Xu, Youming Lu, Sirong Hu, Hao Xia, Wenjun Liu, Dao Hua Zhang, Peijiang Cao, Ming Fang, School of Electrical and Electronic Engineering, and LUMINOUS! Centre of Excellence for Semiconductor Lighting & Displays
- Subjects
Materials science ,Uv detector ,business.industry ,Fast speed ,Electromagnetic Radiation ,General Chemical Engineering ,Detector ,Metal Nanoparticles ,General Chemistry ,Article ,Chemistry ,Simple (abstract algebra) ,Electrical and electronic engineering [Engineering] ,Optoelectronics ,Mixed phase ,business ,QD1-999 - Abstract
High-performance solar-blind UV detector with high response and fast speed is needed in multiple types of areas, which is hard to achieve in one device with a simple structure and device fabrication process. Here, the effects of Ag nanoparticles (NPs) with different sizes on UV response characteristics of the device are studied, the Ag NPs with different sizes that are made from a simple vacuum anneal method. Ag NPs with different sizes could modulate the peak response position of the mixed-phase MgZnO detector from near UV range (350 nm) to deep UV range (235 nm), and the enhancement effect of the Ag NPs on the UV response differs much with the crystal structure and the basic UV response of the MgZnO thin film. When high density 20-40 nm Ag NPs is induced, the deep UV (235 nm) response of the mixed-phase MgZnO detector is increased by 226 times, the I uv/I dark ratio of the modified device is increased by 17.5 times. The slight enhancement in UV light intensity from 20 to 40 nm Ag NPs induces multiple tunnel breakdown phenomena within the mixed-phase MgZnO thin film, which is the main reason for the abnormal great enhancement effect on deep UV response of the device, so the recovery speed of the modified device is not influenced. Therefore, Ag NPs with different sizes could effectively modulate the UV response peak position of mixed-phase MgZnO thin films, and the introduction of Ag NPs with high density and small size is a simple way to greatly increase the sensitivity of the mixed-phase MgZnO detector at deep UV light without decreasing the device speed. Published version This work was supported by the National Natural Science Foundation of China under grant nos. (51872187, 11774241, 51371120, 51302174, 61704111, 61504083, and 61574051), the Natural Science Foundation of Guangdong Province (2016A030313060 and 2017A030310524), the Public Welfare Capacity Building in Guangdong Province (2015A010103016), the Project of Department of Education of Guangdong Province (2014KTSCX110), the Science and Technology Research Items of Shenzhen (JCYJ20170818144255777, JCYJ20170818144212483, JCYJ20180507182248925, JCYJ201602261920, JCYJ20170818143417082, and JCYJ20160226192033020), the Science and Technology Foundation of Shenzhen, and the National Key Research and Development Program of China (2017YFB0404100 and 2017YFB0403000).
- Published
- 2021
26. miR-135a-5p mediates memory and synaptic impairments via the Rock2/Adducin1 signaling pathway in a mouse model of Alzheimer’s disease
- Author
-
Jie Zheng, Ke Cui, Chuan Lai, Zhi-Tao Han, Youming Lu, Hong-hong Zhang, Yazhuo Hu, Kai Zheng, Dan Liu, Wan Xiong, Fan Hu, Heng-Ye Man, Jian-Guo Chen, Ling-Qiang Zhu, Yang Zhou, and Juan Zhang
- Subjects
0301 basic medicine ,Male ,Transgene ,Science ,General Physics and Astronomy ,Mice, Transgenic ,tau Proteins ,Hippocampal formation ,Biology ,Hippocampus ,General Biochemistry, Genetics and Molecular Biology ,Article ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Alzheimer Disease ,microRNA ,Animals ,ROCK2 ,Phosphorylation ,Maze Learning ,Cells, Cultured ,Mice, Knockout ,Neurons ,Memory Disorders ,rho-Associated Kinases ,Multidisciplinary ,Cognitive neuroscience ,General Chemistry ,Mice, Inbred C57BL ,Cytoskeletal Proteins ,Disease Models, Animal ,MicroRNAs ,030104 developmental biology ,Synapses ,Excitatory postsynaptic potential ,Diseases of the nervous system ,Signal transduction ,Neuroscience ,030217 neurology & neurosurgery ,Signal Transduction - Abstract
Aberrant regulation of microRNAs (miRNAs) has been implicated in the pathogenesis of Alzheimer’s disease (AD), but most abnormally expressed miRNAs found in AD are not regulated by synaptic activity. Here we report that dysfunction of miR-135a-5p/Rock2/Add1 results in memory/synaptic disorder in a mouse model of AD. miR-135a-5p levels are significantly reduced in excitatory hippocampal neurons of AD model mice. This decrease is tau dependent and mediated by Foxd3. Inhibition of miR-135a-5p leads to synaptic disorder and memory impairments. Furthermore, excess Rock2 levels caused by loss of miR-135a-5p plays an important role in the synaptic disorder of AD via phosphorylation of Ser726 on adducin 1 (Add1). Blocking the phosphorylation of Ser726 on Add1 with a membrane-permeable peptide effectively rescues the memory impairments in AD mice. Taken together, these findings demonstrate that synaptic-related miR-135a-5p mediates synaptic/memory deficits in AD via the Rock2/Add1 signaling pathway, illuminating a potential therapeutic strategy for AD., Several micro RNAs have been shown to be deregulated in brain tissue or sera from individuals with Alzheimer’s disease and in AD mouse models. The authors show that miR-135a-5p is downregulated in excitatory pyramidal neurons from AD mice and that dysfunction of miR-135a-5p/Rock2/Add1 results in memory/synaptic disorder in AD.
- Published
- 2021
27. Enhanced urea oxidization electrocatalysis on spinel cobalt oxide nanowires via on-site electrochemical defect engineering
- Author
-
Deliang Zhu, Shun Han, Ming Fang, Youming Lu, Wangying Xu, Peijiang Cao, Wen-Bo Xu, and Wenjun Liu
- Subjects
Electrolysis ,Materials science ,Electrolyte ,Electrochemistry ,Electrocatalyst ,Catalysis ,law.invention ,Reaction rate ,Chemical engineering ,law ,Materials Chemistry ,General Materials Science ,Cobalt oxide ,Hydrogen production - Abstract
Urea electrolysis provides a promising approach for simultaneous energy-saving hydrogen production and wastewater utilization; however, the total reaction rate is severely restricted by the sluggish kinetics of the anodic urea oxidization reaction (UOR). Herein, we report a universal and highly controllable electrochemical defect-engineering approach to enrich the active sites on transition metal oxides toward promoted UOR catalysis. A large number of oxygen vacancies (Vo) were introduced on the surface of a Co3O4 nanowire model-material through negative potential tuning, leading to significantly increased UOR activity of the material. At a potential of 1.34 V vs. RHE, the defect-engineered catalyst delivers an optimal UOR current density of 100 mA cm−2, which is much higher than that (42 mA cm−2) offered by the original Co3O4 catalyst, and outperforms the best reported noble-metal-free UOR catalysts. Experimental results further reveal that the formation of Vo can effectively reduce the charge transport/transfer resistances both inside the nanowires and at the catalyst/electrolyte interface, giving rise to improved UOR activity. These findings suggest the great potential of this electrochemical defect-engineering strategy in the design of defect-enriched catalysts for advanced electrocatalysis.
- Published
- 2021
28. Loss of ferroportin induces memory impairment by promoting ferroptosis in Alzheimer’s disease
- Author
-
Dong Xie, Kai Chen, Ling-Qiang Zhu, Wan Xiong, Youming Lu, Jian-Guo Chen, Peter T. Nelson, Zhi Tao Han, Wen Dai Bao, Wang-Xia Wang, Heng-Ye Man, Jing Wang, Fudi Wang, Xiao Ting Zhou, Dan Liu, Fan Hu, Ya Zhuo Hu, Pei Pang, and Hong Hong Zhang
- Subjects
Programmed cell death ,Memory Disorders ,Neocortex ,Ferroportin ,Hippocampus ,Cell Biology ,Biology ,Neural ageing ,Article ,Pathogenesis ,Ageing ,Disease Models, Animal ,Mice ,medicine.anatomical_structure ,In vivo ,Alzheimer Disease ,biology.protein ,medicine ,Cancer research ,Memory impairment ,Animals ,Ferroptosis ,Humans ,Molecular Biology ,Intracellular - Abstract
Iron homeostasis disturbance has been implicated in Alzheimer’s disease (AD), and excess iron exacerbates oxidative damage and cognitive defects. Ferroptosis is a nonapoptotic form of cell death dependent upon intracellular iron. However, the involvement of ferroptosis in the pathogenesis of AD remains elusive. Here, we report that ferroportin1 (Fpn), the only identified mammalian nonheme iron exporter, was downregulated in the brains of APPswe/PS1dE9 mice as an Alzheimer’s mouse model and Alzheimer’s patients. Genetic deletion of Fpn in principal neurons of the neocortex and hippocampus by breeding Fpnfl/fl mice with NEX-Cre mice led to AD-like hippocampal atrophy and memory deficits. Interestingly, the canonical morphological and molecular characteristics of ferroptosis were observed in both Fpnfl/fl/NEXcre and AD mice. Gene set enrichment analysis (GSEA) of ferroptosis-related RNA-seq data showed that the differentially expressed genes were highly enriched in gene sets associated with AD. Furthermore, administration of specific inhibitors of ferroptosis effectively reduced the neuronal death and memory impairments induced by Aβ aggregation in vitro and in vivo. In addition, restoring Fpn ameliorated ferroptosis and memory impairment in APPswe/PS1dE9 mice. Our study demonstrates the critical role of Fpn and ferroptosis in the progression of AD, thus provides promising therapeutic approaches for this disease.
- Published
- 2021
29. Preparation of high performance Ga2O3 based ultraviolet photodetector by CVD
- Author
-
Xiao Wang, Qiao Cheng, Ximing Rong, Shun Han, Yuxiang Zeng, Peijiang Cao, Ming Fang, Wenjun Liu, Deliang Zhu, and Youming Lu
- Subjects
Inorganic Chemistry ,Materials Chemistry ,Ceramics and Composites ,Physical and Theoretical Chemistry ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials - Published
- 2023
30. Aqueous Solution-Processed Nanometer-Thin Crystalline Indium Ytterbium Oxide Thin-Film Transistors
- Author
-
Wangying Xu, Chuyu Xu, Liping Hong, Fang Xu, Chun Zhao, Yu Zhang, Ming Fang, Shun Han, Peijiang Cao, Youming Lu, Wenjun Liu, and Deliang Zhu
- Subjects
General Chemical Engineering ,General Materials Science ,aqueous solution-processed ,indium ytterbium oxide ,ultra-thin ,thin-film transistors ,bias stress stability - Abstract
We demonstrate the growth of ultra-thin (~5 nm) indium ytterbium oxide (In-Yb-O) thin film using a simple vacuum-free aqueous solution approach for the first time. The influences of Yb addition on the microstructural, chemical, optical, and electrical properties of In2O3 are well investigated. The analyses indicate that Yb dopant could suppress oxygen vacancy defects effectively owing to the lower standard electrode potential, lower electronegativity, and stronger metal-oxide bond strength than that of In. The optimized In-Yb-O thin-film transistors (TFTs) exhibit excellent electrical performance (mobility of 8 cm2/Vs and on/off ratio of ~108) and enhanced stability. The triumph of In-Yb-O TFTs is owing to the high quality In2O3 matrix, the remarkable suppressor of Yb, and the nanometer-thin and atomically smooth nature (RMS: ~0.26 nm) of channel layer. Therefore, the eco-friendly water-induced ultra-thin In-Yb-O channel provides an excellent opportunity for future large-scale and cost-effective electronic applications.
- Published
- 2022
31. Water-Processed Ultrathin Crystalline Indium-Boron-Oxide Channel for High-Performance Thin-Film Transistor Applications
- Author
-
Wangying Xu, Tao Peng, Yujia Li, Fang Xu, Yu Zhang, Chun Zhao, Ming Fang, Shun Han, Deliang Zhu, Peijiang Cao, Wenjun Liu, and Youming Lu
- Subjects
In-B-O ,thin-film transistors ,crystalline ,water processed ,ultrathin ,atomically smooth ,ZrO2 dielectric ,General Chemical Engineering ,General Materials Science - Abstract
Thin-film transistors (TFTs) made of solution-processable transparent metal oxide semiconductors show great potential for use in emerging large-scale optoelectronics. However, current solution-processed metal oxide TFTs still suffer from relatively poor device performance, hindering their further advancement. In this work, we create a novel ultrathin crystalline indium–boron–oxide (In-B-O) channel layer for high-performance TFTs. We show that high-quality ultrathin (~10 nm) crystalline In-B-O with an atomically smooth nature (RMS: ~0.15 nm) could be grown from an aqueous solution via facile one-step spin-coating. The impacts of B doping on the physical, chemical and electrical properties of the In2O3 film are systematically investigated. The results show that B has large metal–oxide bond dissociation energy and high Lewis acid strength, which can suppress oxygen vacancy-/hydroxyl-related defects and alleviate dopant-induced carrier scattering, resulting in electrical performance improvement. The optimized In-B-O (10% B) TFTs based on SiO2/Si substrate demonstrate a mobility of ~8 cm2/(V s), an on/off current ratio of ~106 and a subthreshold swing of 0.86 V/dec. Furthermore, by introducing the water-processed high-K ZrO2 dielectric, the fully aqueous solution-grown In-B-O/ZrO2 TFTs exhibit excellent device performance, with a mobility of ~11 cm2/(V s), an on/off current of ~105, a subthreshold swing of 0.19 V/dec, a low operating voltage of 5 V and superior bias stress stability. Our research opens up new avenues for low-cost, large-area green oxide electronic devices with superior performance.
- Published
- 2022
32. miR-34b-3p Inhibition of eIF4E Causes Post-stroke Depression in Adult Mice
- Author
-
Xiao Ke, Manfei Deng, Zhuoze Wu, Hongyan Yu, Dian Yu, Hao Li, Youming Lu, Kai Shu, and Lei Pei
- Subjects
Physiology ,General Neuroscience ,General Medicine - Abstract
Post-stroke depression (PSD) is a serious and common complication of stroke, which seriously affects the rehabilitation of stroke patients. To date, the pathogenesis of PSD is unclear and effective treatments remain unavailable. Here, we established a mouse model of PSD through photothrombosis-induced focal ischemia. By using a combination of brain imaging, transcriptome sequencing, and bioinformatics analysis, we found that the hippocampus of PSD mice had a significantly lower metabolic level than other brain regions. RNA sequencing revealed a significant reduction of miR34b-3p, which was expressed in hippocampal neurons and inhibited the translation of eukaryotic translation initiation factor 4E (eIF4E). Furthermore, silencing eIF4E inactivated microglia, inhibited neuroinflammation, and abolished the depression-like behaviors in PSD mice. Together, our data demonstrated that insufficient miR34b-3p after stroke cannot inhibit eIF4E translation, which causes PSD by the activation of microglia in the hippocampus. Therefore, miR34b-3p and eIF4E may serve as potential therapeutic targets for the treatment of PSD.
- Published
- 2022
33. A congenital CMV infection model for follow-up studies of neurodevelopmental disorders, neuroimaging abnormalities, and treatment
- Author
-
Yue-Peng Zhou, Meng-Jie Mei, Xian-Zhang Wang, Sheng-Nan Huang, Lin Chen, Ming Zhang, Xin-Yan Li, Hai-Bin Qin, Xiao Dong, Shuang Cheng, Le Wen, Bo Yang, Xue-Fang An, Ao-Di He, Bing Zhang, Wen-Bo Zeng, Xiao-Jun Li, Youming Lu, Hong-Chuang Li, Haidong Li, Wei-Guo Zou, Alec J. Redwood, Simon Rayner, Han Cheng, Michael A. McVoy, Qiyi Tang, William J. Britt, Xin Zhou, Xuan Jiang, and Min-Hua Luo
- Subjects
Infectious disease ,Mice, Inbred ICR ,Neurodevelopment ,Neuroimaging ,General Medicine ,Mouse models ,Disease Models, Animal ,Mice ,Pregnancy ,Virology ,Cytomegalovirus Infections ,Animals ,Female ,Research Article ,Follow-Up Studies - Abstract
Congenital cytomegalovirus (cCMV) infection is the leading infectious cause of neurodevelopmental disorders. However, the neuropathogenesis remains largely elusive due to a lack of informative animal models. In this study, we developed a congenital murine CMV (cMCMV) infection mouse model with high survival rate and long survival period that allowed long-term follow-up study of neurodevelopmental disorders. This model involves in utero intracranial injection and mimics many reported clinical manifestations of cCMV infection in infants, including growth restriction, hearing loss, and impaired cognitive and learning-memory abilities. We observed that abnormalities in MRI/CT neuroimaging were consistent with brain hemorrhage and loss of brain parenchyma, which was confirmed by pathological analysis. Neuropathological findings included ventriculomegaly and cortical atrophy associated with impaired proliferation and migration of neural progenitor cells in the developing brain at both embryonic and postnatal stages. Robust inflammatory responses during infection were shown by elevated inflammatory cytokine levels, leukocyte infiltration, and activation of microglia and astrocytes in the brain. Pathological analyses and CT neuroimaging revealed brain calcifications induced by cMCMV infection and cell death via pyroptosis. Furthermore, antiviral treatment with ganciclovir significantly improved neurological functions and mitigated brain damage as shown by CT neuroimaging. These results demonstrate that this model is suitable for investigation of mechanisms of infection-induced brain damage and long-term studies of neurodevelopmental disorders, including the development of interventions to limit CNS damage associated with cCMV infection.
- Published
- 2022
34. Aqueous solution deposition of amorphous gallium tin oxide for thin-film transistors applications
- Author
-
Shun Han, Peijiang Cao, Wangying Xu, Deliang Zhu, Youming Lu, Wenjun Liu, Lingjiao Zhang, and Ming Fang
- Subjects
010302 applied physics ,Aqueous solution ,Materials science ,Process Chemistry and Technology ,chemistry.chemical_element ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Tin oxide ,01 natural sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,law.invention ,Amorphous solid ,chemistry ,Chemical engineering ,Thin-film transistor ,law ,0103 physical sciences ,Materials Chemistry ,Ceramics and Composites ,Thin film ,Crystallization ,Gallium ,0210 nano-technology ,Tin - Abstract
Amorphous gallium tin oxide (GTO) thin films were prepared by a facile, and eco-friendly, aqueous solution process and their applications in thin-film transistors (TFTs) were investigated. The success of the aqueous route was realized by introducing tin fluoride (SnF2) instead of the traditional tin chloride (SnCl2) solute. The roles of adding Ga on the GTO’s microstructural, optical, chemical, and electrical performance were comprehensively studied. It was found that Ga can frustrate the crystallization while it augments the optical bandgap (Eg) value of pristine SnO2. More importantly, using Ga could effectively inhibit the formation of oxygen vacancies, thus greatly improving the electrical performance of the GTO TFTs. The amorphous GTO TFTs with a Ga content of 45% exhibited optimum performance, with a saturation mobility (μsat) of 4.26 cm2/V s, an on/off current ratio (Ion/Ioff) >107, and a decent bias stress durability.
- Published
- 2020
35. Solar-Blind Photodetector with Lower Dark Current and Higher Ilight/Idark Ratio Based on Mg0.38Zn0.62O Film Deposited by Pulsed Laser Deposition Method
- Author
-
Xinke Liu, Wangying Xu, Qing Wang, Shun Han, Peijiang Cao, Zeng Yuxiang, Deliang Zhu, Youming Lu, Ming Fang, Wenjun Liu, and Ch. N. Rao
- Subjects
Materials science ,Band gap ,business.industry ,Biomedical Engineering ,Photodetector ,Bioengineering ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Cutoff frequency ,Pulsed laser deposition ,Responsivity ,Phase (matter) ,Surface roughness ,Optoelectronics ,General Materials Science ,0210 nano-technology ,business ,Dark current - Abstract
In this study, pulsed laser deposition method (PLD) was employed to grow MgxZn1-xO films on quartz substrates. The optimal deposition temperature of 300 °C for MgxZn1-xO film was decided and Mg0.38Zn0.62O, Mg0.56Zn0.44O and Mg0.69Zn0.31O films were grown respectively using MgxZn1-xO targets with different Mg contents (x = 0.3, 0.5 and 0.7). As-deposited Mg0.38Zn0.62O film possessed the mixed-phase (hexagonal and cubic phase) structure, appropriate band gap of 4.68 eV and smaller surface roughness of 1.72 nm, and the solar-blind photodetector (PD) based on it was fabricated. The key features of our PD are the cutoff wavelength of 265 nm lying in solar-blind band, lower dark current (Idark) of 88 pA, higher peak responsivity of 0.10 A/W and bigger Ilight/Idark ratio of 1688, which provide the new idea for the application of solar-blind PDs based on MgxZn1-xO films.
- Published
- 2020
36. Aqueous Solution-Processed Boron-Doped Gallium Oxide Dielectrics for High-Performance Thin-Film Transistors
- Author
-
Shun Han, Youming Lu, Deliang Zhu, Peijiang Cao, Lin Chen, Wen-Jun Liu, Ming Fang, and Wangying Xu
- Subjects
inorganic chemicals ,Chemical substance ,Materials science ,genetic structures ,Oxide ,02 engineering and technology ,Dielectric ,010402 general chemistry ,01 natural sciences ,law.invention ,chemistry.chemical_compound ,Magazine ,law ,Physical and Theoretical Chemistry ,Aqueous solution ,business.industry ,Transistor ,equipment and supplies ,021001 nanoscience & nanotechnology ,eye diseases ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,General Energy ,chemistry ,Thin-film transistor ,Optoelectronics ,sense organs ,0210 nano-technology ,business ,Science, technology and society - Abstract
In this paper, we report aqueous solution-processed boron-doped gallium oxide dielectric thin films for high-performance oxide thin-film transistors. The role of B incorporation on the microstructu...
- Published
- 2020
37. Ag Localized Surface Plasma-Modulated NBE Emissions from ZnCdO Thin Films
- Author
-
Peijiang Cao, Wenjun Liu, Youming Lu, Shaobing Wu, Shun Han, Zeng Yuxiang, Deliang Zhu, Wangying Xu, Xi Cheng, and Ming Fang
- Subjects
010302 applied physics ,Photoluminescence ,Materials science ,business.industry ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Evaporation (deposition) ,Electronic, Optical and Magnetic Materials ,law.invention ,Pulsed laser deposition ,Semiconductor ,law ,0103 physical sciences ,Materials Chemistry ,Optoelectronics ,Quantum efficiency ,Electrical and Electronic Engineering ,Thin film ,0210 nano-technology ,business ,Localized surface plasmon ,Light-emitting diode - Abstract
Recently, metal surface plasma technology has been widely used to improve the quantum efficiency of optoelectronic devices, such as ZnO light-emitting diodes (LEDs). In this work, Ag films with the appropriate thickness were prepared by evaporation and annealed, and a layer of ZnCdO films with different Cd concentrations was grown on the Ag films by the pulsed laser deposition method. During this process, the changes in the Cd concentrations caused the near-band edge (NBE) emission of the ZnCdO films to be continuously adjusted in the ultraviolet to green wavelength range by controlling the oxygen pressure. The prepared Ag nanoparticles and ZnCdO composite films were used to study the metal localized surface plasmon (LSP) effect. The results show that the enhanced or reduced photoluminescence intensity of ZnCdO NBE depends on the probability of electrons transport to either the LSP level or the Fermi level of the metal. This discovery provides an important theoretical basis for the preparation of ZnCdO semiconductor LEDs in the future.
- Published
- 2020
38. High Sensitivity NO2 Gas Sensor Based on 3D WO3 Microflowers Assembled by Numerous Nanoplates
- Author
-
Ch. N. Rao, Shun Han, Xinke Liu, Zeng Yuxiang, Deliang Zhu, Meng Li, Peijiang Cao, Ming Fang, Wangying Xu, Wenjun Liu, and Youming Lu
- Subjects
Pore size ,Detection limit ,Reproducibility ,Materials science ,Biomedical Engineering ,Bioengineering ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Hydrothermal circulation ,Chemical engineering ,Operating temperature ,Oxidizing agent ,General Materials Science ,0210 nano-technology ,Selectivity ,Sensitivity (electronics) - Abstract
Tungsten oxide microflowers (WO3 MFs) were fabricated by a simple hydrothermal process through adjusting the pH of the solution by HCl. These MFs possess the outer diameters of about 2 μm and are composed of numerous nanoplates with the average pore size of 10.9 nm. Chemiresistive activity of as-fabricated WO3 MFs sensor was attempted towards oxidizing and reducing target gases, revealing a superior selectivity to NO2 with a maximum response of 22.95 (2 ppm NO2) @105 °C compared to other target gases. One of the key features of as-fabricatedWO3 MFs sensor is the lower detection limit of 125 ppb and operating temperature of 105 °C to NO2 with better reproducibility, signifying commercial prospective of the developed sensor materials. Finally, the gas sensing mechanism of WO3 MFs sensor has been proposed.
- Published
- 2020
39. High-performance monolayer MoS2 photodetector enabled by oxide stress liner using scalable chemical vapor growth method
- Author
-
Youming Lu, Xinke Liu, Cong Wang, Han Zhang, Jing Wu, Wenjie Yu, and Zhiwen Li
- Subjects
Materials science ,QC1-999 ,Oxide ,Photodetector ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Nanomaterials ,Stress (mechanics) ,chemistry.chemical_compound ,al2o3 ,Monolayer ,Electrical and Electronic Engineering ,photodetector ,stress liner ,Physics ,Vapor growth ,monolayer mos2 ,021001 nanoscience & nanotechnology ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,chemistry ,0210 nano-technology ,Biotechnology - Abstract
MoS2, as a typical representative of two-dimensional semiconductors, has been explored extensively in applications of optoelectronic devices because of its adjustable bandgap. However, to date, the performance of the fabricated photodetectors has been very sensitive to the surrounding environment owing to the large surface-to-volume ratio. In this work, we report on large-scale, high-performance monolayer MoS2 photodetectors covered with a 3-nm Al2O3 layer grown by atomic layer deposition. In comparison with the device without the Al2O3 stress liner, both the photocurrent and responsivity are improved by over 10 times under 460-nm light illumination, which is due to the tensile strain induced by the Al2O3 layer. Further characterization demonstrated state-of-the-art performance of the device with a responsivity of 16.103 A W−1, gain of 191.80, NEP of 7.96 × 10−15 W Hz−1/2, and detectivity of 2.73 × 1010 Jones. Meanwhile, the response rise time of the photodetector also reduced greatly because of the increased electron mobility and reduced surface defects due to the Al2O3 stress liner. Our results demonstrate the potential application of large-scale strained monolayer MoS2 photodetectors in next-generation imaging systems.
- Published
- 2020
40. Electroluminescence Properties of a Zinc Oxide Nanorod Array Heterojunction Light-Emitting Diode
- Author
-
Shun Han, Zeng Yuxiang, Deliang Zhu, Wangying Xu, Hua Jiang, Youming Lu, Ming Fang, Peijiang Cao, Wenjun Liu, and Ximing Rong
- Subjects
010302 applied physics ,Materials science ,Valence (chemistry) ,business.industry ,Heterojunction ,02 engineering and technology ,Electroluminescence ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Electronic, Optical and Magnetic Materials ,Pulsed laser deposition ,law.invention ,law ,0103 physical sciences ,Materials Chemistry ,Optoelectronics ,Nanorod ,Electrical and Electronic Engineering ,0210 nano-technology ,business ,Excitation ,Diode ,Light-emitting diode - Abstract
A p-GaN/i-MgO/n-ZnO nanorod array (NRA) heterojunction light-emitting diode has been fabricated. Its room-temperature electroluminescence spectra under different forward biases revealed a strong emission band across the whole visible region, which was blue-shifted when increasing the forward bias. The origin of this visible emission is considered to be related to oxygen vacancy (VO) defects in different valence states, where the blue emission (∼ 460 nm) comes from neutral oxygen vacancy (VOX ) defects and the green emission (∼ 520 nm) from singly charged oxygen vacancy (VO+ ) defects, while the yellow (∼ 580 nm) and red emission (∼ 670 nm) are attributed to doubly charge oxygen vacancy (VO++ ) defects. These VO defects in different states can convert into one another under different excitation conditions, resulting in the blue-shift of the emission peak as the forward voltage is increased.
- Published
- 2020
41. Temperature-Dependent Crystallization of Ga2O3 for Ultraviolet Photodetectors
- Author
-
Zeng Yuxiang, Deliang Zhu, Ximing Rong, Jinjie Wu, Youming Lu, Peijiang Cao, Wenjun Liu, Shun Han, and Chao Li
- Subjects
010302 applied physics ,Materials science ,business.industry ,Scanning electron microscope ,Band gap ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Electronic, Optical and Magnetic Materials ,law.invention ,Pulsed laser deposition ,Amorphous solid ,Absorption edge ,law ,0103 physical sciences ,Materials Chemistry ,Optoelectronics ,Electrical and Electronic Engineering ,Crystallization ,Thin film ,0210 nano-technology ,business ,Dark current - Abstract
As an n-type oxide semiconductor, Ga2O3 shows great promise in solar-blind ultraviolet (UV) photodetectors due to its adequate band gap, high absorption coefficient, high thermal and chemical stability, high breakdown voltage, and high sensitivity to UV light. In order to investigate the photoresponse of the Ga2O3 photodetector with different crystallization status, we fabricated a series of β-Ga2O3 thin films on a sapphire substrate via pulsed laser deposition with different deposition temperatures from 250°C to 650°C. X-ray diffraction and scanning electron microscopy result showed crystallization enhancement due to the increasing of deposition temperature, and the crystallization procedure started at ∼ 450°C. Optical analysis indicated a blue shift present at the absorption edge (from 4.6 eV to 5.1 eV) with temperature increasing from 450°C to 650°C, showing modulation feasibility in the band gap due to temperature dependence. In addition, all samples showed high transmittance (over 90%) over the entire visible spectrum. Film detectors fabricated by these samples showed high photoresponse and high light/dark current ratio (ILight/IDark) on Ga2O3 deposited at 450°C. Transmission electron microscopy result for film deposited at 450°C showed that the top and bottom regions of films contained both amorphous and crystalline Ga2O3, while there was no crystallization in the middle area of the film, indicating that a combined amorphous-crystalline Ga2O3 film with adequate ratio of crystalline state can significantly enhance the photoresponse while restraining the dark current.
- Published
- 2020
42. High-mobility nanometer-thick crystalline In–Sm–O thin-film transistors via aqueous solution processing
- Author
-
Wenjun Liu, Youming Lu, Peijiang Cao, Ming Fang, Wangying Xu, Deliang Zhu, Shun Han, and Yanwei Li
- Subjects
010302 applied physics ,Materials science ,Dopant ,business.industry ,Doping ,Transistor ,02 engineering and technology ,General Chemistry ,Dielectric ,021001 nanoscience & nanotechnology ,01 natural sciences ,law.invention ,Threshold voltage ,law ,Thin-film transistor ,Printed electronics ,0103 physical sciences ,Materials Chemistry ,Optoelectronics ,Nanometre ,0210 nano-technology ,business - Abstract
Thin-film transistors (TFTs) based on solution-derived metal oxides hold great potential in emerging low-cost large-area printed electronics. Despite recent impressive progress, these device performances are far behind those of their corresponding vacuum-based counterparts, impeding their future commercialization. In this work, we designed and created high-performance TFTs based on a nanometer-thick (down to 5 nm) crystalline In–Sm–O channel via aqueous solution processing, with a performance comparable to those of existing vacuum-processed metal oxides. The microstructural, chemical, optical, and electrical properties of the ultra-thin In–Sm–O samples as a function of Sm doping content (0–10%) were comprehensively investigated. The In–Sm–O TFTs (5% Sm) on SiO2/Si dielectrics demonstrated state-of-the-art performance, including a high mobility of 21.51 ± 1.33 cm2 V−1 s−1, subthreshold swing of 0.66 ± 0.06 V per decade, threshold voltage of 2.14 ± 0.44 V, on/off current ratio >108, and remarkable bias stress stability. The success of In–Sm–O was attributed to the high quality of the crystalline In2O3 matrix, the ideal nature of Sm dopant in suppressing oxygen vacancies, as well as the ultrathin and atomically smooth nature of the channel layer. Therefore, the aqueous solution-processed ultra-thin In–Sm–O channel is expected to enable the realization of future low-cost, large-area, and high-performance green electronics.
- Published
- 2020
43. Self-Powered Au/MgZnO/Nanolayered Ga-Doped ZnO/In Metal–Insulator–Semiconductor UV Detector with High Internal Gain at Deep UV Light under Low Voltage
- Author
-
Youming Lu, Ming Fang, Deliang Zhu, Wangying Xu, Shun Han, Peijiang Cao, Haojie Zhang, and Wenjun Liu
- Subjects
Semiconductor ,Materials science ,Uv detector ,business.industry ,Doping ,Detector ,Optoelectronics ,General Materials Science ,Metal insulator ,business ,Low voltage ,Pulsed laser deposition - Abstract
Au/MgZnO/nanolayered ZnO (Ga-doped)/In MIS (metal–insulator–semiconductor) structure UV detectors are fabricated by the PLD (pulsed laser deposition) method, and the effect of different thickness G...
- Published
- 2019
44. p-Type transparent amorphous oxide thin-film transistors using low-temperature solution-processed nickel oxide
- Author
-
Shun Han, Youming Lu, Xinke Liu, Yujia Li, Junpeng Zhang, Peijiang Cao, Lin Chen, Wangying Xu, Deliang Zhu, Lingjiao Zhang, and Wenjun Liu
- Subjects
Electron mobility ,Materials science ,Annealing (metallurgy) ,business.industry ,Mechanical Engineering ,Nickel oxide ,Non-blocking I/O ,Metals and Alloys ,Oxide ,02 engineering and technology ,Dielectric ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Amorphous solid ,chemistry.chemical_compound ,chemistry ,Mechanics of Materials ,Thin-film transistor ,Materials Chemistry ,Optoelectronics ,0210 nano-technology ,business - Abstract
Transparent amorphous oxide semiconductor (TAOS) represented by a-InGaZnO (IGZO) has achieved their commercial success in the display industry due to its superior material properties (e.g. high mobility (>10 cm 2 V −1 s −1 ), good transparency and excellent uniformity). However, TAOSs with excellent electrical properties are all n-type due to the particular electronic structures of oxide materials. The absence of high performance p-type TAOS limits the device application such as complimentary circuit and is now the largest drawback for oxide electronics. Here, we propose a low-temperature solution method to fabricate p-type transparent amorphous nickel oxide (NiO). The influence of processing parameters such as annealing temperature, precursor concentration, source/drain electrode, and dielectric layer is systematically investigated to maximum the NiO device performance. The optimized NiO TFT exhibits outstanding p-channel behavior, including a high hole mobility of 6.0 cm 2 V −1 s −1 , remarkable on/off current modulation ratio of ∼10 7 , and good subthreshold swing of 0.13 V/decade. The high performance NiO device is attributed to the synergistic optimization of annealing temperature, channel thickness, source/drain electrodes, and dielectric materials. The properties of our p-type TAOS are comparable with that of traditional n-type TAOS.
- Published
- 2019
45. Principal Phosphor Materials and Their Optical Properties
- Author
-
Youming Lu, Weizhen Liu, Cen Zhang, Haiyang Xu, Yichun Liu, Dan Lu, Song Liang, Ming Li, Lingjuan Zhao, Yugang Zeng, Yongqiang Ning, Lijun Wang, Zhijue Quan, Jianli Zhang, Fengyi Jiang, Hao Long, Baoping Zhang, Xinqiang Wang, Yan Peng, Xiangang Xu, Wenna Du, and Xinfeng Liu
- Published
- 2021
46. Synaptic dysfunction of Aldh1a1 neurons in the ventral tegmental area causes impulsive behaviors
- Author
-
Xian Huang, Youming Lu, Yumei Ding, Tongmei Zhang, Hao Li, Quntao Yu, Qing Tian, Wei Jing, Xinyan Li, Wenting Chen, and Hongyan Yu
- Subjects
Male ,Glutamate decarboxylase ,Delay of gratification ,Neurotransmission ,Biology ,Inhibitory postsynaptic potential ,Synaptic Transmission ,Mice ,Cellular and Molecular Neuroscience ,Postsynaptic potential ,medicine ,Animals ,RC346-429 ,Molecular Biology ,Aldh1a1 neurons ,Neurons ,Ventral Tegmental Area ,Synaptic circuits ,RC952-954.6 ,Glutamate receptor ,Long-term potentiation ,Ventral tegmental area ,Impulsive behaviors ,medicine.anatomical_structure ,Geriatrics ,Impulsive Behavior ,Synapses ,Excitatory postsynaptic potential ,Neurology. Diseases of the nervous system ,Neurology (clinical) ,Alzheimer’s disease ,Neuroscience ,Research Article - Abstract
Background Aldh1a1 neurons are a subtype of gamma-aminobutyric acid (GABA) inhibitory neurons that use Aldh1a1 rather than glutamate decarboxylase (GAD) as an enzyme for synthesizing GABA transmitters. However, the behaviors and circuits of this newly identified subtype of inhibitory interneurons remain unknown. Methods We generated a mutant mouse line in which cyclization recombination enzyme (CRE) was expressed under the control of the Aldh1a1 promotor (Aldh1a1-CRE mice). Using this mutant strain of mice together with the heterozygous male Alzheimer’s disease (AD) related model mice (APPswe/PSEN1dE9, or AD mice) and a genetically modified retrograde and anterograde synaptic tracing strategy, we have studied a specific synaptic circuit of Aldh1a1 neurons with system-level function and disease progression in AD mice. Results We demonstrate that Aldh1a1 neurons encode delay of gratification that measures self-control skills in decision making by projecting inhibitory synapses directly onto excitatory glutamate neurons in the intermediate lateral septum (EGNIS) and receiving synaptic inputs from layer 5b pyramidal neurons in the medial prefrontal cortex (L5PN). L5PN → Aldh1a1 synaptic transmission undergoes long-term potentiation (LTP). Pathway specific inhibition by either genetic silencing presynaptic terminals or antagonizing postsynaptic receptors impairs delay of gratification, resulting in the impulsive behaviors. Further studies show that reconstitution of Aldh1a1-deficient neurons with the expression of exogenous Aldh1a1 (eAldh1a1) restores Aldh1a1 → EGNIS synaptic transmission and rescues the impulsive behaviors in AD mice. Conclusions These results not only identify a specific function and circuit of Aldh1a1 neurons but also provide a cellular point of entry to an important but understudied synaptic mechanism for the induction of impulsive behaviors at an early stage of AD.
- Published
- 2021
47. A selective degeneration of cholinergic neurons mediated by NRADD in an Alzheimer's disease mouse model
- Author
-
Lanfang Li, Bing Zhang, Xiaomei Tang, Quntao Yu, Aodi He, Youming Lu, and Xinyan Li
- Published
- 2022
48. Molecularly defined and functionally distinct cholinergic subnetworks
- Author
-
Xinyan, Li, Hongyan, Yu, Bing, Zhang, Lanfang, Li, Wenting, Chen, Quntao, Yu, Xian, Huang, Xiao, Ke, Yunyun, Wang, Wei, Jing, Huiyun, Du, Hao, Li, Tongmei, Zhang, Liang, Liu, Ling-Qiang, Zhu, and Youming, Lu
- Subjects
Mice ,Prosencephalon ,General Neuroscience ,Cholinergic Agents ,Humans ,Animals ,Hippocampus ,Cholinergic Neurons - Abstract
Cholinergic neurons in the medial septum (MS) constitute a major source of cholinergic input to the forebrain and modulate diverse functions, including sensory processing, memory, and attention. Most studies to date have treated cholinergic neurons as a single population; as such, the organizational principles underling their functional diversity remain unknown. Here, we identified two subsets (D28K
- Published
- 2022
49. PINK1-mediated Drp1
- Author
-
Qingtao, Gao, Runyi, Tian, Hailong, Han, Jesse, Slone, Caifang, Wang, Xiao, Ke, Tongmei, Zhang, Xiangyu, Li, Yuhong, He, Panlin, Liao, Fang, Wang, Ye, Chen, Shiqing, Fu, Kexuan, Zhang, Fangfang, Zeng, Yingxuan, Yang, Zhuo, Li, Jieqiong, Tan, Jiada, Li, Youming, Lu, Taosheng, Huang, Zhonghua, Hu, and Zhuohua, Zhang
- Subjects
Dynamins ,Mice, Knockout ,Mice ,Animals ,Phosphorylation ,Mitochondrial Dynamics ,Protein Kinases ,Mitochondria - Abstract
Dynamic change of mitochondrial morphology and distribution along neuronal branches are essential for neural circuitry formation and synaptic efficacy. However, the underlying mechanism remains elusive. We show here that Pink1 knockout (KO) mice display defective dendritic spine maturation, reduced axonal synaptic vesicles, abnormal synaptic connection, and attenuated long-term synaptic potentiation (LTP). Drp1 activation via S616 phosphorylation rescues deficits of spine maturation in Pink1 KO neurons. Notably, mice harboring a knockin (KI) phosphor-null Drp1
- Published
- 2021
50. Proteomics and functional study reveal kallikrein-6 enhances communicating hydrocephalus
- Author
-
Lei Yuan, Dongdong Zou, Xia Yang, Xin Chen, Youming Lu, Aijun Zhang, Pengqi Zhang, and Fance Wei
- Subjects
Clinical Biochemistry ,Molecular Medicine ,General Medicine ,Molecular Biology - Abstract
Background Communicating hydrocephalus (CH) is a common neurological disorder caused by a blockage of cerebrospinal fluid. In this study, we aimed to explore the potential molecular mechanism underlying CH development. Methods Quantitative proteomic analysis was performed to screen the differentially expressed proteins (DEPs) between patients with and without CH. A CH rat model was verified by Hoechst staining, and the co-localization of the target protein and neuron was detected using immunofluorescence staining. Loss-of-function experiments were performed to examine the effect of KLK6 on the synapse structure. Results A total of 11 DEPs were identified, and kallikrein 6 (KLK6) expression was found to be significantly upregulated in patients with CH compared with that in patients without CH. The CH rat model was successfully constructed, and KLK6 was found to be co-localized with neuronal nuclei in brain tissue. The expression level of IL-1β, TNF-α, and KLK6 in the CH group was higher than that in the control group. After knockdown of KLK6 expression using small-interfering RNA (siRNA), the expression levels of synapsin-1 and PSD95 in neuronal cells were increased, and the length, number, and structure of synapses were significantly improved. Following siRNA interference KLK6 expression, 5681 differentially expressed genes (DEGs) were identified in transcriptome profile. The upregulated DEGs of Appl2, Nav2, and Nrn1 may be involved in the recovery of synaptic structures after the interference of KLK6 expression. Conclusions Collectively, KLK6 participates in the development of CH and might provide a new target for CH treatment.
- Published
- 2021
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.