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The article presents a study to determine Aspergillus niger #93;-glucosidase could produce 1,5-anhydro-D-fructose (AF) using maltooligosaccharides as substrates. It mentions the production of AF by GH31, Aspergillus niger ɑ-glucosidase from maltooligosaccharides by elimination reaction. It adds that GH31 catalyze a hydrolytic reaction.

In this article, the author discusses a research related to the characterization of a glycoside hydrolase family 31 ɑ-glucosidase involved in utilization of starch in Podospora anserina. Topics discussed include transglycosylation, heterologous expression in Pichia pastoris, and Podospora anserina is a filamentous fungus belonging to the class of ascomycetes. Other topics discussed include organism used in biological investigations including aging, and cell differentiation.

The article presents a research on variation in substrate specificity of the enzyme family glycoside hydrolase 31-alpha- glucosidases. It states that the difference in enzyme specificity can be attributed to the difference in an aromatic residue of beta-alpha loop 1 in the catalytic domain of the enzyme. It suggests that presence of Tryptophan 324 (Trp 324) at the catalytic loop is closely associated with alpha-1,6 specificity of the enzyme.