1. Temporal dynamics and genomic programming of plasma cell fates
- Author
-
Manakkat Vijay, Godhev Kumar, Zhou, Ming, Thakkar, Kairavee, Rothrauff, Abigail, Chawla, Amanpreet Singh, Chen, Dianyu, Lau, Louis Chi-Wai, Gerges, Peter Habib, Chetal, Kashish, Chhibbar, Prabal, Fan, Jingyu, Das, Jishnu, Joglekar, Alok, Borghesi, Lisa, Salomonis, Nathan, Xu, Heping, and Singh, Harinder
- Abstract
Affinity-matured plasma cells (PCs) of varying lifespans are generated through a germinal center (GC) response. The developmental dynamics and genomic programs of antigen-specific PC precursors remain to be elucidated. Here, using a model antigen in mice, we demonstrate biphasic generation of PC precursors, with those generating long-lived bone marrow PCs preferentially produced in the late phase of GC response. Clonal tracing using single-cell RNA sequencing and B cell antigen receptor sequencing in spleen and bone marrow compartments, coupled with adoptive transfer experiments, reveals a new PC transition state that gives rise to functionally competent PC precursors. The latter undergo clonal expansion, dependent on inducible expression of TIGIT. We propose a model for the proliferation and programming of precursors of long-lived PCs, based on extended antigen encounters in the GC.
- Published
- 2024
- Full Text
- View/download PDF