1. Ultrasonic approach to enhance signal-to-noise in three dimensional microarray
- Author
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Pan, Zhiqiang, Li, Yanqiang, Zhou, Dongrui, Tang, Jing, Zhang, Minghao, Xiao, Pengfeng, and Lu, Zuhong
- Subjects
- *
ULTRASONIC imaging , *POLYACRYLAMIDE , *POLYMERS , *GENETIC polymorphisms - Abstract
Abstract: Three-dimensional polyacrylamide gel microarray (3-D gel microarray) has a strong adsorption for hybridzation probes with fluorescent label, resulting in high fluorescent background noise. The background noise has hampered the broad application of the microarray in scientific research. A low-frequency ultrasound of 40kHz has been successfully employed to efficiently remove the nonspecifically bound fluorescent probes from the 3-D gel microarray. The method can significantly enhance the signal to noise ratio of the 3-D gel microarray and make it work better in single-nucleotide polymorphism genotyping. [Copyright &y& Elsevier]
- Published
- 2008
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