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AbstractTabernaemontana divaricata(L.) R. Br is a medicinal plant which is blongs to the family of Apocyanaceae, the whole plant extract possess many biological activities such as antioxidant activity, antiinflammatory activity and it has a very good local anesthetic and anti cancer activity. Therefore, the present study is an attempt to evaluate the present of secondary metabolites from ethanolic extract of Tabernaemontana divaricata(L.) using HPTLC analysis which was carried out to find the alkaloids, flavonoids, glycosides, poly phenols, steroids and tannins. In the results, the selected secondary metabolites were present in the ethanolic extract of T. divaricata(L.). Based on the results, this study can be concluded that this plant has variety of biological activities may be presence of alkaloids, flavonoids, glycosides, poly phenols, steroids and tannins in it. In future by isolating and identifying these compounds may be formulated to treat the various diseases.

AbstractFree radical generation is directly associated with oxidation in foods and biological systems. Antioxidants are vital substances which possess the ability to protect the body from damage caused by free radical induced oxidative stress. The aim of the present study is to evaluate and compare the in vitroantioxidant and free radical scavenging capacity of crude and alkaloid extracts of Hybanthus enneaspermus. Antioxidants such as catalase, peroxidase, superoxide dismutase, vitamin C, vitamin E and lipid peroxidation assays were analyzed in both the extracts. The content of total tannins, phenols, flavonoids, carotenoids and alkaloids were estimated and radical scavenging activity was assayed using 1,1-diphenyl-2-picryl hydroxyl (DPPH), 2,2’ azinobis-3 ethylbenzothiozoline-6 sulfonic acid (ABTS+) cation decolourization test, superoxide radical, nitric oxide, hydroxyl radical (OH.), hydrogen peroxide (H2O2), ferric reducing antioxidant power (FRAP) and 2 2 reducing power methods. The results confirm that the crude and alkaloid extracts of H. enneaspermusrevealed high level of secondary metabolites and antioxidant activities. H. enneaspermushas significant antioxidant properties and could serve as a good source of natural antioxidant, which may be helpful in preventing the distructive process of various oxidative stress.