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Alkynes can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans. In addition to the clickable chemical reactivity, alkynes possess a unique Raman scattering within the Raman-silent region of a cell. Coupling this spectroscopic signature with Raman microscopy yields a new imaging modality beyond fluorescence and label-free microscopies. The bioorthogonal Raman imaging of various biomolecules tagged with an alkyne by a state-of-the-art Raman imaging technique, stimulated Raman scattering (SRS) microscopy, is reported. This imaging method affords non-invasiveness, high sensitivity, and molecular specificity and therefore should find broad applications in live-cell imaging. [ABSTRACT FROM AUTHOR]

Direct detection of glycans on live cells using surface‐enhanced Raman scattering (SERS) has been shown. A bioorthogonal Raman reporter was directly installed onto the monosaccharide analogs. Once metabolically incorporated into cell surface glycans, the Raman reporter was detected using SERS (see picture). [ABSTRACT FROM AUTHOR]

Raman detection of glycans on surfaces of live cells has been achieved, as described in the Communication by Z.‐Q. Tian, X. Chen, and co‐workers on page 7266 ff. A bioorthogonal Raman reporter (for alkyne, azide, and nitrile groups, or carbon–deuterium bonds) was installed onto the monosaccharide analogues, which were metabolically incorporated into cell surface glycans. The Raman reporter is detected using surface‐enhanced Raman scattering with the aid of gold nanoparticles. [ABSTRACT FROM AUTHOR]