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4 results on '"Magobo, R."'

1. Etest ECVs/ECOFFs for Detection of Resistance in Prevalent and Three Nonprevalent Candida spp. to Triazoles and Amphotericin B and Aspergillus spp. to Caspofungin: Further Assessment of Modal Variability

Author

Espinel-Ingroff, A., primary, Sasso, M., additional, Turnidge, J., additional, Arendrup, M., additional, Botterel, F., additional, Bourgeois, N., additional, Bouteille, B., additional, Canton, E., additional, Cassaing, S., additional, Dannaoui, E., additional, Dehais, M., additional, Delhaes, L., additional, Dupont, D., additional, Fekkar, A., additional, Fuller, J., additional, Garcia-Effron, G., additional, Garcia, J., additional, Gonzalez, G. M., additional, Govender, N. P., additional, Guegan, H., additional, Guinea, J., additional, Houzé, S., additional, Lass-Flörl, C., additional, Pelaez, T., additional, Forastiero, A., additional, Lackner, M., additional, and Magobo, R., additional

Published
2021
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2. Method-Dependent Epidemiological Cutoff Values for Detection of Triazole Resistance in Candida and Aspergillus Species for the Sensititre YeastOne Colorimetric Broth and Etest Agar Diffusion Methods

Author

Espinel-Ingroff, A., primary, Turnidge, J., additional, Alastruey-Izquierdo, A., additional, Botterel, F., additional, Canton, E., additional, Castro, C., additional, Chen, Y.-C., additional, Chen, Y., additional, Chryssanthou, E., additional, Dannaoui, E., additional, Garcia-Effron, G., additional, Gonzalez, G. M., additional, Govender, N. P., additional, Guinea, J., additional, Kidd, S., additional, Lackner, M., additional, Lass-Flörl, C., additional, Linares-Sicilia, M. J., additional, López-Soria, L., additional, Magobo, R., additional, Pelaez, T., additional, Quindós, G., additional, Rodriguez-Iglesia, M. A., additional, Ruiz, M. A., additional, Sánchez-Reus, F., additional, Sanguinetti, M., additional, Shields, R., additional, Szweda, P., additional, Tortorano, A., additional, Wengenack, N. L., additional, Bramati, S., additional, Cavanna, C., additional, DeLuca, C., additional, Gelmi, M., additional, Grancini, A., additional, Lombardi, G., additional, Meletiadis, J., additional, Negri, C. E., additional, Passera, M., additional, Peman, J., additional, Prigitano, A., additional, Sala, E., additional, and Tejada, M., additional

Published
2019
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3. Method-Dependent Epidemiological Cutoff Values for Detection of Triazole Resistance in Candidaand AspergillusSpecies for the Sensititre YeastOne Colorimetric Broth and Etest Agar Diffusion Methods

Author

Espinel-Ingroff, A., Turnidge, J., Alastruey-Izquierdo, A., Botterel, F., Canton, E., Castro, C., Chen, Y.-C., Chen, Y., Chryssanthou, E., Dannaoui, E., Garcia-Effron, G., Gonzalez, G. M., Govender, N. P., Guinea, J., Kidd, S., Lackner, M., Lass-Flörl, C., Linares-Sicilia, M. J., López-Soria, L., Magobo, R., Pelaez, T., Quindós, G., Rodriguez-Iglesia, M. A., Ruiz, M. A., Sánchez-Reus, F., Sanguinetti, M., Shields, R., Szweda, P., Tortorano, A., Wengenack, N. L., Bramati, S., Cavanna, C., DeLuca, C., Gelmi, M., Grancini, A., Lombardi, G., Meletiadis, J., Negri, C. E., Passera, M., Peman, J., Prigitano, A., Sala, E., and Tejada, M.

Abstract

Although the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candidaor Aspergillusspecies. We collected fluconazole, itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171 Candida albicans, 215 C. dubliniensis, 4,418 C. glabrataspecies complex, 157 C.guilliermondii(Meyerozyma guilliermondii), 676 C. krusei(Pichia kudriavzevii), 298 C.lusitaniae(Clavispora lusitaniae), 911 C.parapsilosissensu stricto, 3,691 C.parapsilosisspecies complex, 36 C.metapsilosis, 110 C.orthopsilosis, 1,854 C.tropicalis, 244 Saccharomyces cerevisiae, 1,409 Aspergillus fumigatus, 389 A.flavus, 130 A.nidulans, 233 A.niger, and 302 A.terreuscomplex isolates.

Published
2018
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4. Method-Dependent Epidemiological Cutoff Values for Detection of Triazole Resistance in Candida and Aspergillus Species for the Sensititre YeastOne Colorimetric Broth and Etest Agar Diffusion Methods.

Author

Espinel-Ingroff A, Turnidge J, Alastruey-Izquierdo A, Botterel F, Canton E, Castro C, Chen YC, Chen Y, Chryssanthou E, Dannaoui E, Garcia-Effron G, Gonzalez GM, Govender NP, Guinea J, Kidd S, Lackner M, Lass-Flörl C, Linares-Sicilia MJ, López-Soria L, Magobo R, Pelaez T, Quindós G, Rodriguez-Iglesia MA, Ruiz MA, Sánchez-Reus F, Sanguinetti M, Shields R, Szweda P, Tortorano A, Wengenack NL, Bramati S, Cavanna C, DeLuca C, Gelmi M, Grancini A, Lombardi G, Meletiadis J, Negri CE, Passera M, Peman J, Prigitano A, Sala E, and Tejada M

Subjects
Aspergillosis drug therapy, Aspergillosis epidemiology, Aspergillosis microbiology, Aspergillus classification, Aspergillus isolation & purification, Candida classification, Candida isolation & purification, Candidiasis drug therapy, Candidiasis epidemiology, Candidiasis microbiology, Disk Diffusion Antimicrobial Tests, Drug Resistance, Fungal, Fluconazole pharmacology, Humans, Immunocompromised Host, Itraconazole pharmacology, Voriconazole pharmacology, Antifungal Agents pharmacology, Aspergillus drug effects, Candida drug effects, Triazoles pharmacology
Abstract

Although the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171 Candida albicans , 215  C. dubliniensis , 4,418  C. glabrata species complex, 157  C. guilliermondii ( Meyerozyma guilliermondii ), 676  C. krusei ( Pichia kudriavzevii ), 298  C. lusitaniae ( Clavispora lusitaniae ), 911  C. parapsilosis sensu stricto , 3,691  C. parapsilosis species complex, 36  C. metapsilosis , 110  C. orthopsilosis , 1,854  C. tropicalis , 244 Saccharomyces cerevisiae , 1,409 Aspergillus fumigatus , 389  A. flavus , 130  A. nidulans , 233  A. niger , and 302  A. terreus complex isolates. SYO/Etest MICs for 282 confirmed non-wild-type (non-WT) isolates were included: ERG11 ( C. albicans ), ERG11 and MRR1 ( C. parapsilosis ), cyp51A ( A. fumigatus ), and CDR2 and CDR1 overexpression ( C. albicans and C. glabrata , respectively). Interlaboratory modal agreement was superior by SYO for yeast species and by the Etest for Aspergillus spp. Distributions fulfilling CLSI criteria for epidemiological cutoff value (ECV) definition were pooled, and we proposed SYO ECVs for S. cerevisiae and 9 yeast and 3 Aspergillus species and Etest ECVs for 5 yeast and 4 Aspergillus species. The posaconazole SYO ECV of 0.06 µg/ml for C. albicans and the Etest itraconazole ECV of 2 µg/ml for A. fumigatus were the best predictors of non-WT isolates. These findings support the need for method-dependent ECVs, as, overall, the SYO appears to perform better for susceptibility testing of yeast species and the Etest appears to perform better for susceptibility testing of Aspergillus spp. Further evaluations should be conducted with more Candida mutants., (Copyright © 2018 American Society for Microbiology.)

Published
2018
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