1. α-Fucosidases with different substrate specificities from two species of Fusarium
- Author
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Jonathan D. Walton, Janet M. Paper, John S. Scott-Craig, Mareike Bongers, Richard E. Wiemels, Ahmed Faik, David Cavalier, and Melissa S. Borrusch
- Subjects
alpha-L-Fucosidase ,Genetics ,Subfamily ,Sequence Homology, Amino Acid ,Sequence analysis ,Molecular Sequence Data ,Fungal genetics ,food and beverages ,Sequence alignment ,Sequence Analysis, DNA ,General Medicine ,Biology ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Substrate Specificity ,Pichia pastoris ,Microbiology ,Fusarium ,Fusarium oxysporum ,Glycosides ,DNA, Fungal ,Sequence Alignment ,Gene ,Trichoderma reesei ,Biotechnology - Abstract
Two fungal-secreted α-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of Fusarium oxysporum strain 0685 grown on l-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on p-nitrophenyl-α-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan. A putative α-fucosidase gene (FgFCO1) from Fusarium graminearum was expressed in Pichia pastoris. FgFCO1 was ∼1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share
- Published
- 2012