1. Effect of low-frequency ultrasound on double-strand breaks in giant DNA molecules
- Author
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Yukihiro Kagawa, Kenichi Yoshikawa, Hiraku Tabata, Takahiro Kenmotsu, Naoki Ogawa, Yuko Yoshikawa, Yoshiaki Watanabe, and Kenji Yoshida
- Subjects
Physics and Astronomy (miscellaneous) ,Hydrophone ,Chemistry ,business.industry ,Ultrasound ,Molecular physics ,DNA Strand Break ,Standing wave ,chemistry.chemical_compound ,Nonlinear acoustics ,Nuclear magnetic resonance ,Cavitation ,otorhinolaryngologic diseases ,Sound pressure ,business ,DNA - Abstract
Double-strand breaks in giant DNA molecules caused by continuous ultrasound at a frequency of 30 kHz were quantified using single-molecule observations. The effect of the sound pressure was investigated by placing a tube containing DNA solution under an anti-node of the acoustic standing wave. Almost no breaks occurred below the threshold sound pressure. Above this threshold, the probability of strand breaks increased linearly with sound pressure. Acoustic cavitation detected with a hydrophone strongly suggests that the main mechanism of the DNA strand break is via cavitation generated by the ultrasound.
- Published
- 2013
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