1. MicroRNA-143 inhibits proliferation and migration of prostate cancer cells.
- Author
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Bajhan, Elshan, Mansoori, Behzad, Mohammadi, Ali, Shanehbandi, Dariush, Khaze Shahgoli, Vahid, Baghbani, Elham, Hajiasgharzadeh, Khalil, and Baradaran, Behzad
- Subjects
CANCER cell migration ,MICRORNA ,STAINS & staining (Microscopy) ,CELL migration ,PROSTATE cancer ,CELL growth - Abstract
Background: Prostate cancer (PC) is one of the most prevalent types of malignancies in males. Here, we replaced the miRNA-143 in PC cells by using a vector-based miRNA-143 transfection approach. Materials and methods: The miRNA-143 vector was transfected into the cells and qRT-PCR was applied to assess the expression of target genes in PC3 cells. Also, the MTT, scratch wound-healing, and DAPI staining assays were done to assess the proliferation, migration, and apoptosis of the cells, respectively. Results: The findings of the qRT-PCR determined the enhanced expression of miRNA-143 and other cancer-associated genes. The MTT and wound-healing assays revealed the proliferation and migration reduction in the transfected cells in comparison to control cells that contain an empty vector. Conclusion: The miRNA-143 has a significant impact on cell growth and migration during PC metastasis, and it may be a promising candidate for molecular therapies of PC. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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