1. EFFECT OF HEXANE FRACTION FROM PAPAYA (CARICA PAPAYA L.) MALE FLOWER ON CELL CYCLE OF COLON ADENOCARCINOMA (WIDR) CELL AND ITS COMBINATION INDEX WITH DOXORUBICIN
- Author
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Masfria Masfria, Rosidah R, Iksen Iksen, and Yusnita Y
- Subjects
Pharmacology ,biology ,Chemistry ,Cell ,Pharmaceutical Science ,Combination index ,Fraction (chemistry) ,Cell cycle ,biology.organism_classification ,Molecular biology ,digestive system diseases ,Hexane ,chemistry.chemical_compound ,medicine.anatomical_structure ,mental disorders ,medicine ,Pharmacology (medical) ,Doxorubicin ,Colon adenocarcinoma ,Carica ,medicine.drug - Abstract
Objective: This study aimed to evaluate the effects of papaya (Carica papaya L.) male flower hexane fraction (PHF) on cell cycle of colon adenocarcinoma (WiDr) cell and its combination index (CI) with doxorubicin.Methods: Flow cytometer and the CI were used to show the PHF on cell cycle of colon adenocarcinoma (WiDr) cell and to calculate the synergism potential, respectively.Result: The result showed that the PHF giving inhibition on cell cycle of colon adenocarcinoma (WiDr) cell in G0-G1, S, G2-M phase. Furthermore, the combination of PHF with doxorubicin in colon adenocarcinoma (WiDr) cell gave a strong synergistic effect with optimal concentration of 8 μg/ml–50 nM (PHF-Doxorubicin) with the IC score lower than 1.Conclusion: This study provides evidence that PHF could be a new potential co-chemotherapeutic agent with doxorubicin on colon adenocarcinoma cell.
- Published
- 2018