1. Glycolytic enzyme Enolase-1 regulates insulin gene expression in pancreatic β-cell.
- Author
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Luo, Xiumei, Luan, Cheng, Zhou, Jingqi, Ye, Yingying, Zhang, Wei, Jain, Ruchi, Zhang, Enming, and Chen, Ning
- Subjects
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INSULIN , *GENE expression , *PANCREATIC enzymes , *PANCREATIC beta cells , *TYPE 2 diabetes , *ISLANDS of Langerhans , *GLUCOSE metabolism - Abstract
Enolase-1 (Eno1) plays a critical role in regulating glucose metabolism; however, its specific impact on pancreatic islet β-cells remains elusive. This study aimed to provide a preliminary exploration of Eno1 function in pancreatic islet β-cells. The findings revealed that the expression of ENO1 mRNA in type 2 diabetes donors was significantly increased and positively correlated with HbA1C and negatively correlated with insulin gene expression. A high level of Eno1 in human insulin-secreting rat INS-1832/13 cells with co-localization with intracellular insulin proteins was accordingly observed. Silencing of Eno1 using siRNA or inhibiting Eno1 protein activity with an Eno1 antagonist significantly reduced insulin secretion and insulin content in β-cells, while the proinsulin/insulin content ratio remained unchanged. This reduction in β-cells function was accompanied by a notable decrease in intracellular ATP and mitochondrial cytochrome C levels. Overall, our findings confirm that Eno1 regulates the insulin secretion process, particularly glucose metabolism and ATP production in the β-cells. The mechanism primarily involves its influence on insulin production, suggesting that Eno1 represents a potential target for β-cell protection and diabetes treatment. • A significant increase of ENO1 mRNA expression in type-2 diabetes donors. • Eno1 exhibits co-localization with intracellular insulin in beta cells. • Silencing of Eno1 using siRNA reduces insulin secretion and insulin content. • A decrease in intracellular ATP and mitochondrial cytochrome C levels in the Eno1 silencing beta cells. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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