Search

3-oxoacid CoA-transferase 1 (OXCT1) is a key enzyme in ketone body metabolism that is expressed in adipose and other tissues. The present study addressed the function of OXCT1 in adipose tissue from Tan sheep. The 1563 bp ovine OXCT1 coding sequence was cloned from ovine adipose tissue. The OXCT1 protein sequence was highly homologous to OXCT1 from other species. OXCT1 was highly expressed in kidney and at lower levels in small intestine, lung, spleen, heart, stomach, liver, tail adipose, and cartilage, but not in longissimus muscle. OXCT1 was expressed at higher levels in perirenal and tail adipose tissues than in subcutaneous adipose tissue. OXCT1 expression levels increased during the in vitro differentiation of adipocytes, but decreased dramatically at day 8. OXCT1 knockdown in ovine adipocytes promoted lipid accumulation, whereas overexpression did the converse. This study demonstrates that OXCT1 may play a role in adipogenesis and provides new insight on adipose deposition in sheep. • OXCT1 increases during differentiation of ovine preadipocytes but declines on day 8. • OXCT1 knockdown promotes lipid accumulation and adipocyte differentiation. • OXCT1 overexpression decreases lipid accumulation and inhibits adipogenesis. [ABSTRACT FROM AUTHOR]

Abstract: Here, two temperature sensitive promoters, P2 and P7, isolated from Bacillus subtilis, were characterized. The production of β-galactosidase driven by these promoters was much higher at 45°C than that at 37°C both in Escherichia coli and B. subtilis and that the P2 promoter showed higher expression strength in B. subtilis at 45°C. Thereby, an efficient temperature-inducible expression system was constructed by using P2 promoter in B. subtilis. Thus, we isolated and characterized a newly temperature inducible promoter and exploited it as a potential expression element in B. subtilis. [Copyright &y& Elsevier]