1. IFN-γ down-regulates Secretoglobin 3A1 gene expression
- Author
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Agasa Miyazono, Shioko Kimura, Baohong Zhao, Dai Suzuki, Raymond P. Donnelly, Matsuo Yamamoto, Masamichi Takami, Hiroyuki Itabe, Ryutaro Kamijo, Kumiko Oshima, Atsushi Yamada, and Akihide Kamiya
- Subjects
Biophysics ,Gene Expression ,Mice, Inbred Strains ,Inflammation ,Biochemistry ,Article ,Interferon-gamma ,Mice ,Interferon ,Cell Line, Tumor ,Gene expression ,medicine ,Animals ,STAT1 ,Promoter Regions, Genetic ,Molecular Biology ,Gene ,Receptors, Interferon ,Epithelial cell differentiation ,biology ,Proteins ,Cell Biology ,Recombinant Proteins ,Cell biology ,STAT1 Transcription Factor ,Cell culture ,Cancer research ,biology.protein ,Phosphorylation ,medicine.symptom ,medicine.drug - Abstract
STAT1 mediates Interferon (IFN)-dependent positive and negative regulation of inflammatory gene expression in lung. In this study, we examined the effect of IFN-gamma on the expression of SCGB3A1 which is thought to play crucial roles in inflammation and epithelial cell differentiation in lung. We found that expression of SCGB3A1 was down-regulated by IFN-gamma in a time- and dose-dependent manner in the murine transformed Clara Cells (mtCC) line. IFN-gamma induced the phosphorylation of STAT1, which binds to a STAT-binding element (SBE) in the SCGB3A1 gene promoter, leading to decreased transcriptional activation of this gene.
- Published
- 2009