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Start Over Author "Hailin Cong" Journal biomicrofluidics
4 results on '"Hailin Cong"'

1. Fabrication of anti-protein-fouling poly(ethylene glycol) microfluidic chip electrophoresis by sandwich photolithography

Author

Xiaodan Xu, Hua Yuan, Bing Yu, Hailin Cong, and Huwei Liu

Subjects
Materials science, Microfluidics, Biomedical Engineering, Nanotechnology, 02 engineering and technology, 01 natural sciences, Soft lithography, law.invention, chemistry.chemical_compound, Colloid and Surface Chemistry, law, General Materials Science, Fluid Flow and Transfer Processes, Microchannel, Polydimethylsiloxane, 010401 analytical chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Electrophoresis, chemistry, Photolithography, 0210 nano-technology, Ethylene glycol, Regular Articles, Microfabrication
Abstract

Microfluidic chip electrophoresis (MCE) is a powerful separation tool for biomacromolecule analysis. However, adsorption of biomacromolecules, particularly proteins onto microfluidic channels severely degrades the separation performance of MCE. In this paper, an anti-protein-fouling MCE was fabricated using a novel sandwich photolithography of poly(ethylene glycol) (PEG) prepolymers. Photopatterned microchannel with a minimum resolution of 10 μm was achieved. After equipped with a conventional online electrochemical detector, the device enabled baseline separation of bovine serum albumin, lysozyme (Lys), and cytochrome c (Cyt-c) in 53 s under a voltage of 200 V. Compared with a traditional polydimethylsiloxane MCE made by soft lithography, the PEG MCE made by the sandwich photolithography not only eliminated the need of a master mold and the additional modification process of the microchannel but also showed excellent anti-protein-fouling properties for protein separation.

Published
2016

2. Fabrication of universal serial bus flash disk type microfluidic chip electrophoresis and application for protein analysis under ultra low voltage

Author

Hua Yuan, Bing Yu, Xiaodan Xu, Hailin Cong, and Huwei Liu

Subjects
Materials science, Serial communication, Biomedical Engineering, 02 engineering and technology, USB, 01 natural sciences, Soft lithography, law.invention, Flash (photography), Printed circuit board, Colloid and Surface Chemistry, Capillary electrophoresis, law, Hardware_INTEGRATEDCIRCUITS, General Materials Science, Fluid Flow and Transfer Processes, Microchannel, business.industry, 010401 analytical chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Optoelectronics, 0210 nano-technology, business, Low voltage, Computer hardware, Regular Articles
Abstract

A simple and effective universal serial bus (USB) flash disk type microfluidic chip electrophoresis (MCE) was developed by using poly(dimethylsiloxane) based soft lithography and dry film based printed circuit board etching techniques in this paper. The MCE had a microchannel diameter of 375 μm and an effective length of 25 mm. Equipped with a conventional online electrochemical detector, the device enabled effectively separation of bovine serum albumin, lysozyme, and cytochrome c in 80 s under the ultra low voltage from a computer USB interface. Compared with traditional capillary electrophoresis, the USB flash disk type MCE is not only portable and inexpensive but also fast with high separation efficiency.

Published
2016

3. Fabrication of anti-protein-fouling poly(ethylene glycol) microfluidic chip electrophoresis by sandwich photolithography.

Author

Hailin Cong, Xiaodan Xu, Bing Yu, Huwei Liu, and Hua Yuan

Subjects
*FABRICATION (Manufacturing), *FOULING, *MICROFLUIDICS, *PHOTOLITHOGRAPHY, *ELECTROPHORESIS
Abstract

Microfluidic chip electrophoresis (MCE) is a powerful separation tool for biomacromolecule analysis. However, adsorption of biomacromolecules, particularly proteins onto microfluidic channels severely degrades the separation performance of MCE. In this paper, an anti-protein-fouling MCE was fabricated using a novel sandwich photolithography of poly(ethylene glycol) (PEG) prepolymers. Photopatterned microchannel with a minimum resolution of 10 μm was achieved. After equipped with a conventional online electrochemical detector, the device enabled baseline separation of bovine serum albumin, lysozyme (Lys), and cytochrome c (Cyt-c) in 53 s under a voltage of 200 V. Compared with a traditional polydimethylsiloxane MCE made by soft lithography, the PEG MCE made by the sandwich photolithography not only eliminated the need of a master mold and the additional modification process of the microchannel but also showed excellent anti-protein-fouling properties for protein separation. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
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4. Fabrication of universal serial bus flash disk type microfluidic chip electrophoresis and application for protein analysis under ultra low voltage.

Author

Hailin Cong, Xiaodan Xu, Bing Yu, Huwei Liu, and Hua Yuan

Subjects
*USB technology, *MICROFLUIDIC devices, *ELECTROPHORESIS, *PROTEIN analysis, *ELECTRIC potential, *POLYDIMETHYLSILOXANE, *SOFT lithography, *FABRICATION (Manufacturing)
Abstract

A simple and effective universal serial bus (USB) flash disk type microfluidic chip electrophoresis (MCE) was developed by using poly(dimethylsiloxane) based soft lithography and dry film based printed circuit board etching techniques in this paper. The MCE had a microchannel diameter of 375 μm and an effective length of 25 mm. Equipped with a conventional online electrochemical detector, the device enabled effectively separation of bovine serum albumin, lysozyme, and cytochrome c in 80 s under the ultra low voltage from a computer USB interface. Compared with traditional capillary electrophoresis, the USB flash disk type MCE is not only portable and inexpensive but also fast with high separation efficiency. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

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