1. Fabrication of anti-protein-fouling poly(ethylene glycol) microfluidic chip electrophoresis by sandwich photolithography
- Author
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Xiaodan Xu, Hua Yuan, Bing Yu, Hailin Cong, and Huwei Liu
- Subjects
Materials science ,Microfluidics ,Biomedical Engineering ,Nanotechnology ,02 engineering and technology ,01 natural sciences ,Soft lithography ,law.invention ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,law ,General Materials Science ,Fluid Flow and Transfer Processes ,Microchannel ,Polydimethylsiloxane ,010401 analytical chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,0104 chemical sciences ,Electrophoresis ,chemistry ,Photolithography ,0210 nano-technology ,Ethylene glycol ,Regular Articles ,Microfabrication - Abstract
Microfluidic chip electrophoresis (MCE) is a powerful separation tool for biomacromolecule analysis. However, adsorption of biomacromolecules, particularly proteins onto microfluidic channels severely degrades the separation performance of MCE. In this paper, an anti-protein-fouling MCE was fabricated using a novel sandwich photolithography of poly(ethylene glycol) (PEG) prepolymers. Photopatterned microchannel with a minimum resolution of 10 μm was achieved. After equipped with a conventional online electrochemical detector, the device enabled baseline separation of bovine serum albumin, lysozyme (Lys), and cytochrome c (Cyt-c) in 53 s under a voltage of 200 V. Compared with a traditional polydimethylsiloxane MCE made by soft lithography, the PEG MCE made by the sandwich photolithography not only eliminated the need of a master mold and the additional modification process of the microchannel but also showed excellent anti-protein-fouling properties for protein separation.
- Published
- 2016