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Your search keyword '"microorganism detection"' showing total 3 results
Start Over Descriptor "microorganism detection" Journal bioorganic medicinal chemistry letters
3 results on '"microorganism detection"'

1. Fluorogenic 7-azidocoumarin and 3/4-azidophthalimide derivatives as indicators of reductase activity in microorganisms.

Author

Chalansonnet V, Lowe J, Orenga S, Perry JD, Robinson SN, Stanforth SP, Sykes HE, and Truong TV

Subjects
Coumarins chemical synthesis, Fluorescent Dyes chemical synthesis, Molecular Structure, Phthalimides chemical synthesis, Substrate Specificity, Coumarins chemistry, Fluorescent Dyes chemistry, Gram-Negative Bacteria enzymology, Gram-Positive Bacteria enzymology, Nitroreductases metabolism, Phthalimides chemistry
Abstract

A series of fluorogenic heterocyclic azides were prepared and assessed as reductase substrates across a selection of Gram-negative and Gram-positive microorganisms. The majority of these azides showed similar activity profiles to nitroreductase substrates. Microorganisms that do not produce hydrogen sulfide reduced the azides, indicating reductase activity was not linked to hydrogen sulfide production., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Published
2019
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2. Fluorogenic l-alanylaminopeptidase substrates derived from 6-amino-2-hetarylquinolines and 7-amino-3-hetarylcoumarins and their potential applications in diagnostic microbiology.

Author

Cellier-Rastit M, James AL, Orenga S, Perry JD, Robinson SN, Turnbull G, and Stanforth SP

Subjects
Enzyme Assays, Gram-Negative Bacteria enzymology, Spectrometry, Fluorescence, Substrate Specificity, CD13 Antigens chemistry, Coumarins chemistry, Fluorescent Dyes chemistry, Quinolines chemistry
Abstract

Six novel fluorogenic enzyme substrates for detecting l-alanylaminopeptidase activity in microorganisms have been prepared and evaluated in Columbia agar media. The substrates are l-alanyl derivatives of 6-amino-2-hetarylquinolines and 7-amino-3-hetarylcoumarins. Both the quinoline and coumarin series of substrates produced fluorescence in the presence of Gram-negative microorganisms. In contrast, fluorescence generation in the presence of the Gram-positive microorganisms and yeasts was limited or absent., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Published
2019
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3. 2-(Nitroaryl)benzothiazole and benzoxazole derivatives as fluorogenic substrates for the detection of nitroreductase activity in clinically important microorganisms.

Author

Cellier M, Gignoux A, James AL, Orenga S, Perry JD, Robinson SN, Stanforth SP, and Turnbull G

Subjects
Bacterial Proteins analysis, Benzothiazoles metabolism, Benzoxazoles metabolism, Gram-Negative Bacteria enzymology, Gram-Positive Bacteria enzymology, Nitroreductases analysis, Spectrometry, Fluorescence, Substrate Specificity, Bacterial Proteins metabolism, Benzothiazoles chemistry, Benzoxazoles chemistry, Fluorescent Dyes chemistry, Nitroreductases metabolism
Abstract

A series of carboxy-substituted 2-(nitroaryl)benzothiazole derivatives and carboxy-substituted 2-(nitroaryl)benzoxazole derivatives were prepared and evaluated as potential nitroreductase substrates for the purpose of detecting clinically important microorganisms. Several of the substrates produced highly fluorescent colonies with the majority of a panel of 10 Gram-negative bacteria and also with two of a panel of 8 Gram-positive bacteria., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

Published
2015
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