Search

The article presents a study which investigates the cDNA encoding lanosterol synthase (LS) clone produced from Ganoderma lucidum (G. lucidum) through a homology-based polymerized chain reaction (PCR) method. It discovers that the LS cDNA contains an open-reading frame (ORF) of 2,181 bp while the genomic DNA sequence of LS contains 2,924 bp. It infers that the results of PCR are probably affected by the triterpene biosynthesis regulated network of G. lucidum.

The article presents a study on the characterization and differential expression of a farnesyl-diphosphate synthase gene (GlFPS) from the Basidiomycetous fungus Ganoderma lucidum. It was found that GlFPS cDNA conains an open reading frame of 1083 bp, and its deduced amino acid sequence exhibited a high homology with other fungal FPS genes. GlFPS was also expressed in the mycelium growth stage whereas its transcripts accumulated to high levels rapidly during the process of mushroom primordia.

The article presents a study on cloning and characterization of a gene encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) from Ganoderma lucidum and its functional identification in yeast. It provides a complete nucleotide sequence of the full-length complementary DNA (cDNA) encoding HMGR and its genomic DNA sequence. The study found that the deduced amino acid sequence of G. lucidum HMGR indicated significant homology to the HMGRs from Ustilago maydis and Cryptococcus neoformans.